; and (B) by adding at the end the following: (C) a statement of assurance that a scientifically satisfactory nonanimal method of obtaining the result sought is not available; and . 4. National Center for Alternatives to Animals in Research and Testing (a) Addition to list of institutes and centers Section 401 of the Public Health Service Act ( 42 U.S.C. 281 ) is amended— (1) by redesignating paragraph (25) as paragraph (26); and (2) by inserting after paragraph (24) the following new paragraph: (25) The National Center for Alternatives to Animals in Research and Testing. . (b) Conforming change to number of institutes and centers Section 401(d)(1) of the Public Health Service Act ( 42 U.S.C. 281(d)(1) ) is amended by striking 27 and inserting 28 . (c) Establishment; duties Part E of title IV of the Public Health Service Act ( 42 U.S.C. 287 et seq. ) is amended by inserting after subpart 5 of such part E ( 42 U.S.C. 287c–21 ) the following new subpart: 6 National Center for Alternatives to Animals in Research and Testing 485E. Establishment; duties (a) Establishment Not later than one year after the date of enactment of the Humane and Existing Alternatives in Research and Testing Sciences Act of 2022, the Secretary shall establish a National Center for Alternatives to Animals in Research and Testing (in this subpart referred to as the National Center ) within the National Institutes of Health. The Center shall be headed by a director, who shall be appointed by the Secretary. (b) Purpose The general purpose of the National Center shall be— (1) developing, promoting, and funding alternatives to animal research and testing