; and (2) by inserting after paragraph (24) the following new paragraph: (25) National Center for Alternatives to Animals in Research and Testing. . (b) Conforming change to number of institutes and centers Section 401(d)(1) of the Public Health Service Act ( 42 U.S.C. 281(d)(1) ) is amended by striking 27 and inserting 28 . (c) Establishment; duties Part E of title IV of the Public Health Service Act ( 42 U.S.C. 287 et seq.) is amended by inserting after subpart 5 of such part E ( 42 U.S.C. 287c–21 ) the following new subpart: 6 National Center for Alternatives to Animals in Research and Testing 485E. Establishment; duties (a) Establishment Not later than one year after the date of enactment of the Humane Research and Testing Act of 2020 the Secretary shall establish a National Center for Alternatives to Animals in Research and Testing (in this subpart referred to as the National Center ) within the National Institutes of Health. (b) Purposes The sole purposes of the National Center shall be— (1) developing, promoting, and funding alternatives to animal research and testing; and (2) developing a plan for reducing the number of animals used in federally funded research and testing. (c) Duties The Director of the National Center shall— (1) provide assistance (including funding) to federally funded researchers to incentivize research and testing without the use of animals, based on advanced cell cultures or technology such as 3D organoids, microphysiological systems, induced pluripotent adult stem cell models, in silico modeling, advanced imaging systems, artificial intelligence, and other innovative methods; (2) train and inform scientists about these available methods of research and testing without the use of animals