; and (bb) of $10,000 from each such regional center with 20 or fewer total investors in the preceding fiscal year in its new commercial enterprises. (II) Petition fee Beginning on October 1, 2020, the Secretary shall collect a fee of $1,000 for the Fund with each petition filed under section 204(a)(1)(H) for classification under subparagraph (E). The fee under this subclause is in addition to the fee that the Secretary is authorized to establish and collect for each petition to recover the costs of adjudication and naturalization services under section 286(m). (III) Increases The Secretary may increase the amounts under this clause by prescribing such regulations as may be necessary to ensure that amounts in the Fund are sufficient to carry out the purposes set forth in clause (iii). (iii) Permissible uses of fund The Secretary shall— (I) use not less than 1/3 of the amounts deposited into the Fund for investigations based outside of the United States, including— (aa) monitoring and investigating program-related events and promotional activities; and (bb) ensuring an alien investor’s compliance with subparagraph (L); and (II) use amounts deposited into the Fund— (aa) to detect and investigate fraud or other crimes; (bb) to determine whether regional centers, new commercial enterprises, job-creating entities, and alien investors (and their alien spouses and alien children) comply with the immigration laws; (cc) to conduct audits and site visits; and (dd) as the Secretary determines to be necessary, including monitoring compliance with the requirements under section 7 of the EB–5 Reform and Integrity Act of 2019 . (iv) Failure to pay fee The Secretary of Homeland Security shall— (I) impose a reasonable penalty, which shall be deposited into the Fund, if any regional center does not pay the fee required under clause (ii) within 30 days after the date on which such fee is due