; and (B) a detailed explanation and an analysis of the harms and benefits of such use of animals have been completed. (3) Ensure that— (A) research proposals are reviewed by at least one person who has expertise in non-animal research methods; and (B) reviewers of the research proposals have access to a reference librarian with expertise in evaluating the adequacy of the searches for non-animal methods described in the research proposals. (4) Establish and maintain research proposal guidelines for the following: (A) Conducting thorough searches for non-animal alternatives to the use of animals for biomedical and behavioral research. (B) Carrying out analyses of the harms and benefits of the use of animals in proposed research to assess whether the harms that would be caused to animals in terms of suffering, pain, and distress are justified by the expected outcomes, taking into account ethical considerations and the expected benefits to human beings, animals, or the environment. (5) Establish and maintain animal care guidelines for the following: (A) The proper care of animals to be used in biomedical and behavioral research. (B) The proper treatment of animals while being used in such research. Guidelines under this paragraph shall require— (i) the appropriate use of tranquilizers, analgesics, anesthetics, paralytics, and euthanasia for animals in such research