; and (B) includes— (i) the reason for such separation; and (ii) the stated evidence for such separation. (b) Recommendations for separation by agents or officers (1) In general Not later than 180 days after the date of the enactment of this Act, the Secretary, in consultation with the Secretary of Health and Human Services, shall develop training and guidance, with an emphasis on the best interests of the child, on childhood trauma, attachment, and child development, for use by the agents and officers of U.S. Customs and Border Protection, so as to standardize separations authorized under subsection (a)(1)(C). (2) Annual review Not less frequently than annually, the Secretary of Health and Human Services shall— (A) review the guidance developed under paragraph (1); and (B) make recommendations to the Secretary to ensure that such guidance conforms to current evidence and best practices in child welfare, child development, and childhood trauma. (3) Requirement The guidance developed under paragraph (1) shall incorporate the presumptions described in subsection (c). (4) Additional requirements (A) Evidence-based The guidance and training developed under this subsection shall incorporate evidence-based practices. (B) Training required (i) Initial training All agents and officers of U.S. Customs and Border Protection, on hire, and annually thereafter, shall complete training on adherence to the guidance developed under this subsection. (ii) Annual training All Chief Patrol Agents and Area Port Directors, on hire, and annually thereafter, shall complete— (I) training on adherence to the guidance developed under this subsection