OECD Guidelines for the Testing of Chemicals
Introduction '''OECD Guidelines for the Testing of Chemicals''' ('''OECD TG''') are a set of internationally accepted specifications for the testing of chemicals decided on by the Organisation for Economic Co-operation and Development (OECD). They were first published in 1981.They are split into five sections:* Section 1: Physical Chemical Properties* Section 2: Effects on Biotic Systems* Section 3: Environmental Fate and Behaviour* Section 4: Health Effects* Section 5: Other Test GuidelinesGuidelines are numbered with three digit numbers, the section number being the first number. Sometimes guidelines are suffixed with a letter.Guidelines are under constant review, with guidelines being periodically updated, new guidelines being adopted, and guidelines being withdrawn. Previous guidelines are maintained on the website for reference purposes. Animal welfare concerns are dealt with by ensuring that animal tests are only permitted where necessary.The guidelines are available in both English and French.
List of guidelines ===Section 1: Physical Chemical Properties===NumberTitle101  UV-VIS absorption spectra102  Melting point/Melting range103  Boiling point104  Vapour pressure105  Water solubility106  Adsorption – Desorption Using a Batch Equilibrium Method107  Partition coefficient (''n''-octanol/water): Shake Flask Method108  Complex formation ability in water109  Density of liquids and solids110  Particle size distribution/ fibre length and diameter distributions111  Hydrolysis as a function of pH112  Dissociation constants in water113  Screening test for thermal stability and stability in air114  Viscosity of liquids115  Surface tension of aqueous solutions116  Fat solubility of solid and liquid substances117  Partition coefficient (''n''-octanol/water), HPLC Method118  Determination of the Number-Average molecular weight and the molecular weight distribution of polymers using Gel Permeation Chromatography119  Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography120  Solution/Extraction Behaviour of Polymers in Water121  Estimation of the adsorption coefficient (''K''OC) on Soil and on sewage sludge using High Performance Liquid Chromatography (HPLC)122  Determination of pH, Acidity and Alkalinity123  Partition coefficient (1-Octanol/Water): Slow-Stirring Method===Section 2: Effects on Biotic Systems===NumberTitle201  Freshwater Alga and Cyanobacteria, Growth Inhibition Test202  Daphnia sp. Acute Immobilisation Test203  Fish, Acute Toxicity Test204  Fish, Prolonged Toxicity Test: 14-Day Study205  Avian Dietary Toxicity Test206  Avian Reproduction Test207  Earthworm, Acute Toxicity Tests208  Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test209  Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)210  Fish, Early-life Stage Toxicity Test211  Daphnia magna Reproduction Test212  Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages213  Honeybees, Acute Oral Toxicity Test214  Honeybees, Acute Contact Toxicity Test215  Fish, Juvenile Growth Test216  Soil Microorganisms: Nitrogen Transformation Test217  Soil Microorganisms: Carbon Transformation Test218  Sediment-Water Chironomid Toxicity Using Spiked Sediment219  Sediment-Water Chironomid Toxicity Using Spiked Water220  Enchytraeid Reproduction Test221  Lemna sp. Growth Inhibition Test222  Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)223  Avian Acute Oral Toxicity Test224  Determination of the Inhibition of the Activity of Anaerobic Bacteria225  Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment226  Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil227  Terrestrial Plant Test: Vegetative Vigour Test228  Determination of Developmental Toxicity of a Test Chemical to Dipteran Dung Flies (Scathophaga stercoraria L. (Scathophagidae), Musca autumnalis De Geer (Muscidae))229  Fish Short Term Reproduction Assay230  21-day Fish Assay231  Amphibian Metamorphosis Assay232  Collembolan Reproduction Test in Soil233  Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment234  Fish Sexual Development Test235  Chironomus sp., Acute Immobilisation Test236  Fish Embryo Acute Toxicity (FET) Test237  Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure238  Sediment-Free Myriophyllum Spicatum Toxicity Test239  Water-Sediment Myriophyllum Spicatum Toxicity Test240  Medaka Extended One Generation Reproduction Test (MEOGRT)241  The Larval Amphibian Growth and Development Assay (LAGDA)===Section 3: Environmental Fate and Behaviour===NumberTitle301  Ready Biodegradability302A  Inherent Biodegradability: Modified SCAS Test302B  Inherent Biodegradability: Zahn-Wellens/ EVPA Test302C  Inherent Biodegradability: Modified MITI Test (II)303  Simulation Test – Aerobic Sewage Treatment – A: Activated Sludge Units; B: Biofilms304A  Inherent Biodegradability in Soil305  Bioaccumulation in Fish: Aqueous and Dietary Exposure306  Biodegradability in Seawater307  Aerobic and Anaerobic Transformation in Soil308  Aerobic and Anaerobic Transformation in Aquatic Sediment Systems309  Aerobic Mineralisation in Surface Water – Simulation Biodegradation Test310  Ready Biodegradability – CO2 in sealed vessels (Headspace Test)311  Anaerobic Biodegradability of Organic Compounds in Digested Sludge: by Measurement of Gas Production312  Leaching in Soil Columns313  Estimation of Emissions from Preservative – Treated Wood to the Environment314  Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater315  Bioaccumulation in Sediment-dwelling Benthic Oligochaetes316  Phototransformation of Chemicals in Water – Direct Photolysis317  Bioaccumulation in Terrestrial Oligochaetes===Section 4: Health Effects===NumberTitle401  Acute Oral Toxicity402  Acute Dermal Toxicity403  Acute Inhalation Toxicity404  Acute Dermal Irritation/Corrosion405  Acute Eye Irritation/Corrosion406  Skin Sensitisation407  Repeated Dose 28-day Oral Toxicity Study in Rodents408  Repeated Dose 90-Day Oral Toxicity Study in Rodents409  Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents410  Repeated Dose Dermal Toxicity: 21/28-day Study411  Subchronic Dermal Toxicity: 90-day Study412  Subacute Inhalation Toxicity: 28-Day Study413  Subchronic Inhalation Toxicity: 90-day Study414  Prenatal Development Toxicity Study415  One-Generation Reproduction Toxicity Study416  Two-Generation Reproduction Toxicity417  Toxicokinetics418  Delayed Neurotoxicity of Organophosphorus Substances Following Acute Exposure419  Delayed Neurotoxicity of Organophosphorus Substances: 28-day Repeated Dose Study420  Acute Oral Toxicity – Fixed Dose Procedure421  Reproduction/Developmental Toxicity Screening Test422  Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test423  Acute Oral toxicity – Acute Toxic Class Method424  Neurotoxicity Study in Rodents425  Acute Oral Toxicity: Up-and-Down Procedure426  Developmental Neurotoxicity Study427  Skin Absorption: In Vivo Method428  Skin Absorption: In Vitro Method429  Skin Sensitisation430  In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER)431  In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method432  In Vitro 3T3 NRU Phototoxicity Test435  In Vitro Membrane Barrier Test Method for Skin Corrosion436  Acute Inhalation Toxicity – Acute Toxic Class Method437  Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage438  Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage439  In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method440  Uterotrophic Bioassay in Rodents441  Hershberger Bioassay in Rats442A  Skin Sensitization442B  Skin Sensitization442C  In Chemico Skin Sensitisation442D  In Vitro Skin Sensitisation443  Extended One-Generation Reproductive Toxicity Study451  Carcinogenicity Studies452  Chronic Toxicity Studies453  Combined Chronic Toxicity/Carcinogenicity Studies455  Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists456  H295R Steroidogenesis Assay457  BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists460  Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants471  Bacterial Reverse Mutation Test473  In Vitro Mammalian Chromosomal Aberration Test474  Mammalian Erythrocyte Micronucleus Test475  Mammalian Bone Marrow Chromosomal Aberration Test476  In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes477  Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster478  Rodent Dominant Lethal Test479  Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells480  Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay481  Genetic Toxicology: Saacharomyces cerevisiae, Miotic Recombination Assay482  Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro483  Mammalian Spermatogonial Chromosomal Aberration Test484  Genetic Toxicology: Mouse Spot Test485  Genetic toxicology, Mouse Heritable Translocation Assay486  Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo487  In Vitro Mammalian Cell Micronucleus Test488  Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays489  In Vivo Mammalian Alkaline Comet Assay490  In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene491  Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage492  Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage493  Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity===Section 5: Other Test Guidelines===NumberTitle501  Metabolism in Crops502  Metabolism in Rotational Crops503  Metabolism in Livestock504  Residues in Rotational Crops (Limited Field Studies)505  Residues in Livestock506  Stability of Pesticide Residues in Stored Commodities507  Nature of the Pesticide Residues in Processed Commodities – High Temperature Hydrolysis508  Magnitude of the Pesticide Residues in Processed Commodities509  Crop Field Trial
External links *  OECD Guidelines for the Testing of Chemicals