Totarol
Introduction New Zealand scientist Sir Thomas Hill Easterfield was the first person to discover totarol. Photo 1920. '''Totarol''' is a naturally produced diterpene that is bioactive as '''totarol'''. It was first isolated by McDowell and Easterfield from the heartwood of ''Podocarpus totara'', a conifer tree found in New Zealand. Podocarpus totara was investigated for unique molecules due to the tree's increased resistance to rotting. Recent studies have confirmed totarol's unique antimicrobial and therapeutic properties. Consequently, totarol is a candidate for a new source of drugs and has been the goal of numerous syntheses.
Discovery Totarol was discovered in 1910 by New Zealand scientist Sir Thomas Hill Easterfield. While investigating the properties of ''Miro'', ''Kahikatea'', ''Rimu'', ''Matai'' and ''Totara'', Easterfield detected a "crystalline bloom" on totara boards a few hours after leaving the planing machine. After extraction of totarol from ''Podocarpus totara,'' Easterfield observed no other compound had been cited in chemical literature before with this formula. Easterfield and his colleague J.C McDowell proposed the name “totarol” in a follow-up paper in 1915, as the crystalline substance was believed to possess a tertiary alcohol group. In 1937 Short and Stromberg continued investigations, publishing ''Totarol Part 1''. In 1951 Short and Wang became the first to identify the chemical structure of totarol with their paper ''Totarol Part 2''.
Occurrence Although totarol was first isolated in ''Podocarpus totara'', totarol has also been identified in numerous other species of Podocarpaceae and Cupressaceae, with the majority found in the genus ''Podocarpus'' of the family Podocarpaceae and the subfamily Cupressoideae of the family Cupressaceae.Outside Podocarpus and Cupressoideae, totarol is rarely found in the plant kingdom. However, totarol has recently been isolated in ''Rosmarinus officinalis'' (rosemary). The gymnosperms that contain totarol are distributed worldwide but are concentrated in North America, the far-south regions of South America, East Asia and East Africa.
Biological activity === Antimicrobial activity ===Totarol motivates research in drug discovery due to its ability to inhibit numerous microorganisms. Totarol exhibits antimicrobial properties in numerous species including gram-positive bacteria, acid-fast bacteria, nematodes, parasitic protozoans, crustaceous foulers (Table 1). In addition to inhibiting microorganisms by itself, totarol exhibits inhibitory synergy with currently used antimicrobial drugs: totarol potentiates isonicotinic acid hydrazide against various Mycobacteria. ; methicillin against ''Mycobacterium tuberculosis'' and ''Staphylococcus aureus''; and anacardic acid and erythromycin against ''Staphylococcus aureus''. In nature, totarol is a key player in gymnosperm's defense against harmful microbes: gymnosperms that produce totarol are resistant to rotting.Table 1.Antibacterial activity of totarol against microorganisms Microorganism MIC (μg/ml) IC50(μg/ml) ''Artemia salina''   ''Bacterium ammoniagenes''   ''Bacillus subtilis''   ''Caenorhabditis elegans''   ''Enterococcus faecalis''   ''Klebsiella pneumoniae''   ''Mycobacterium aurum''   ''Mycobacterium fortuitum''   ''Mycobacterium phlei''   ''Mycobacterium smegmatis''   ''Mycobacterium tuberculosis H37Rv''   ''Leishmania donovani''   ''Proprionibacterium acnes''   ''Staphylococcus aureus ATCC 12598''   ''Staphylococcus aureus ATCC 33591''   ''Staphylococcus aureus ATCC 11632''   ''Streptococcus mutans''   ''Streptococcus pneumoniae''  === Mechanism of antimicrobial inhibition ===Although totarol exhibits antimicrobial properties, the mode of action is unclear and various methods of inhibitory action have been proposed. In ''Staphylococcus aureus'' strains resistant to penicillin via creation of penicillin binding protein 2’ (PBP2’), totarol may inhibit the synthesis of PBP2’. Totarol may inhibit effluxing ''Staphylococcus aureus'' strains through inhibition of MsrA, although it is unclear if MsrA is an efflux pump. Totarol may also gain its antibacterial properties by inhibiting bacterial respiratory transport but this is very unlikely because totarol is also effective against anaerobic organisms. Recently totarol was also hypothesized to inhibit gram-positive and acid-fast bacteria via inhibition of FtsZ protein, which forms the Z-ring, a polymer necessary for efficient bacterial cell cytokinesis.Totarol may also function by disrupting the structural integrity of the phospholipid bilayer of bacteria by weakening Van der Waals interactions with its phenolic group, which also results in bacterial cells unable to synthesize ATP. Motivation for totarol functioning via disruption of membrane structure is due to its high phospholipid/water partition coefficient. However, totarol's partitioning capability was only observed at concentrations 10 to 100 fold higher than required for antibacterial activity. Thus it is unlikely that totarol is an uncoupler of bacterial respiration at the low levels observed in antimicrobial studies.=== Traditional use ===The use of Podocarpus totara extract in Maori medicines for treatment of fevers, asthma, coughs, cholera, distemper, chest complaints and venereal disease dates back to over 100 years. The timber of Podocarpus totara is renowned for its resilience against rotting, which made it valuable to Maori for housing, waka (canoes), fencing, pa stockades, drinking vessels, shovels and carvings in chiefs houses. Totara bark was used to cover kelp bags used for preserved muttonbirds. European settlers of New Zealand used the wood for wharf piles, bridges, railway sleepers, telegraph poles, lighthouses, mining equipment, fence posts and foundation blocks. The durability stems from the anti-bacterial activity of totarol. Houses, churches, grave markers, and even cobbles and kerbs were made of totara; strips of the bark were used as a roofing material. The presence of totarol means totara wood resists decay and insect attack in the heart timber. Totarol has been found in organic matter in the embankments of a neolithic site in Northern Sweden. Totarol from Cupressaceae resin was possibly used for its antibacterial and antifungal properties to preserve meat, as well as for its ability to repel insects. Despite totarol's antimicrobial potential, its commercial use is currently limited to cosmetic purposes. For totarol to be used clinically, its mode of action needs to be clearly defined.=== Biochemical properties ===Totarol decreases the plasma levels of estrogens and can also effectively reduce pathogenic hepatic cells ''in vitro''. Totarol's anti-cancer activity is hypothesized to be due to the natural product's ability to form an ''o''-quinone methide ''in vivo''. Totarol also prevents cells from undergoing oxidative stress ''in vitro'' by acting as a hydrogen donor to peroxy radicals or reacting with other peroxy radicals to terminate undesirable radical reactions.
Biosynthesis Totarol is a precursor to the formation of nagilactones that possess antifungal properties not possessed by totarol. Consequently, gymnosperms that produce totarol and nagilactones are able to defend themselves against bacteria and fungi.Scheme 1.The initially proposed biosynthetic route of totarol.The biosynthesis of totarol was difficult to determine. The main reason for the challenge in determining how the secondary metabolite is produced is because totarol does not follow the isoprene rule: the isopropyl group of totarol is in the “wrong” place at C14.Initially, it was hypothesized that totarol and the “normal” diterpene ferruginol, also found in Podocarpaceae, were derived by a precursor ''2'' that would be dehydrated and have its isopropyl group migrate to produce totarol 1 and ferruginol  '''3''' (Scheme 1). This hypothesis was motivated by the well known santonin-desmotroposantonin rearrangement of steroid dienones into aromatic compounds. It is now accepted that totarol is synthesized biologically from ferruginol. Geranyl geranyl pyrophosphate '''4''' undergoes typical diterpene cyclization to form (−)-abietadiene '''5''', which is oxidized to form ferruginol '''3''', which proceeds through a spiro intermediate to form totarol (Scheme 2).Scheme 2.The currently accepted biosynthetic route of totarol
Synthesis Scheme 3.The first total synthesis of (±)-totarolTotarol has been the subject of numerous syntheses. The first total synthesis of totarol (Scheme 3) utilized '''6''' and the alkyne '''7''' to yield '''8''' which was converted to the corresponding ketone '''9''' via hydrogenation followed by cyclization with polyphosphoric acid. '''9''' was subsequently converted to '''10''' and another ketone that were inseparable by chromatography. The synthesis was finalized by treatment with N-Bromosuccinimide followed by debromination to yield (±)-totarol. The main downfall of this synthesis was that in multiple steps, complete conversion of reactant to products was not observed and undesirable side products were often not separable by chromatography. However, since this was the first total synthesis of (±)-totarol, it is notable.=== Total enantioselective synthesis ===Scheme 4.The first total enantioselective synthesis of totarolThe first total enantioselective synthesis of totarol was achieved in 1979 (Scheme 4). The key step in the synthesis is the formation of '''13''' via a Wittig reaction between '''11''' and '''12'''. This same cyclization can also be achieved via a Friedel-Crafts alkylation and cyclization. Subsequent hydrogenation of '''13''' followed by intramolecular cyclization with aluminium chloride forms the B ring and totarylmethyl ether which is demethylated by boron tribromide to yield totarol.Scheme 5.The total synthesis of (+) totarol from zamoranic acid.A more recent organic synthesis of totarol was achieved by utilizing '''14''', a lamdane diterpene named zamoranic acid (Scheme 5). The addition of the isopropyl group in the chemical synthesis was achieved with complete stereoselectivity. Acetylation to yield '''15''' required high temperatures due to the steric hindrance of the isopropyl group. Cis-hydroxylation followed by cleavage with H5IO6 yielded a diol that was acylated in pyridine and oxidized to give '''16'''. The key step in the synthesis was the cyclization of ring C: '''16''' was treated with SmI2 to yield totarane diastereomers which were separated by column chromatography. The desired diastereomer was treated with ''p''-TsOH in benzene to yield '''17'''. The synthesis was completed by a halogenation-dehydrogenation sequence and subsequent bromination to yield '''18''' and ring aromatization with elimination via a lithium complex.=== Total chemoenzymatic synthesis ===Scheme 6.The chemoenzymatic synthesis of totarol.Chemoenzymatic synthesis of totarol has also been achieved with high yield (41.8%) (Scheme 6). A racemic beta-keto ester '''19''' undergoes lipase-assisted resolution to yield chiral alcohol '''20'''. Treatment of '''20''' with 10% HCl and p-TsOH gives ''αβ''-unsaturated ketone '''21'''. A Michael addition with the anion obtained from the reaction of methyl 5-methyl-3-oxohexanoate '''13''' with NaOMe gives a 2:1 diastereomeric mixture of '''22''' which is hydrolyzed to yield '''23''' which is brominated and debrominated to yield totarol.
Research +YearSubject1992Bacteria killer and antibiotic enhancer1996Anti methicillin-resistant Staphylococcus aureus1997Antioxidant1998Diterpene1999Antibacterial1999Anti methicillin-resistant Staphylococcus aureus2000 Mosquito insecticide2001Tuberculosis2001Cell change2002Insect repellant2003Antiplasmodial and cytotoxic2004Antimalarial2005Anti inflammatory2006Anti acne2007Antifungal2007Staphyloccocus auereus inhibition2007Tuberculosis2015Neurological disorders2015Bacillus subtilis alteration2017Ultrasound treatment on whey protein-totarol nanoparticles2017Surgical site infection2018Plant defense2018 Food preservative 2019Historical food preservative2019Antibacterial for dental implants2020Whey protein based tissue adhesive2020Mastitis2021Anticancer  2022Drug delivery destroys microbial biofilm2022Anti inflammatory2023Insecticide2023Influenza A
Extraction Totarol is extracted by supercritical extraction. The process uses high pressure carbon dioxide under specific temperature, pressure and gas flow conditions to extract totarol from powdered totara wood. Totarol can be extracted from dead wood, negating the need to cut down live trees. Although totarol can be extracted from other Podocarpus, some trees in the cypress family (cypress, juniper, thuja) and from rosemary, it is most abundant in Podocarpus totara.
Products Products for sale containing totarol include toothpastes, tooth tablets, mouthwash, toners, cleansers, moisturisers, face masks, concealers, blemish control, anti-acne, pimple patches, face cream, eye cream, sun screen, deodorants, face mists, facial wash, anti-wrinkle, restorative serum, renew cream, scar removal serum, dandruff control, scalp treatment, shampoo bar, pet skin cream, hand wash, hand cream, pressed powder makeup, mascara, rescue cream, skin whitener, lip tint, pregnancy body oil, mouth freshener, sore throat relief, cold and flu nasal spray.
Other uses Totarol may also be used as an indicator for the quality of juniper berry based spirits. Juniper berries that contain diterpenoids including totarol are used for the aromatization and production of some gins. Consequently, totarol can aid in the characterization of different types of gin or commercial brands, vouching for the authenticity and quality of the product.Totarol has been found on the posterior tibia of ''Frieseomelitta silvestrii languida'', a species of stingless bees from Brazil. ''Frieseomelitta silvestrii languida'' collect resin to create a protective barrier around the opening of their nest to ward off insects from settling near the nest's entrance. The presence of totarol can aid in the determination of this bee species.
References