## Protocol Section
### Identification Module
**NCT ID:** NCT06417593

**Brief Title:** Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals

**Official Title:** Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals

#### Organization Study ID Info
**ID:** Soh-Med-24-04-016MS
#### Organization
**Class:** OTHER
**Full Name:** Sohag University
### Status Module
#### Completion Date
**Date:** 2025-03-01

**Type:** ESTIMATED

#### Expanded Access Info
#### Last Update Post Date
**Date:** 2024-05-20

**Type:** ACTUAL

**Last Update Submit Date:** 2024-05-16

**Overall Status:** RECRUITING

#### Primary Completion Date
**Date:** 2024-11-01

**Type:** ESTIMATED

#### Start Date
**Date:** 2024-04-01

**Type:** ACTUAL

**Status Verified Date:** 2024-05

#### Study First Post Date
**Date:** 2024-05-16
**Type:** ACTUAL
**Study First Submit Date:** 2024-05-11
**Study First Submit QC Date:** 2024-05-11
### Sponsor Collaborators Module

#### Lead Sponsor

**Class:** OTHER

**Name:** Sohag University

#### Responsible Party

**Investigator Affiliation:** Sohag University

**Investigator Full Name:** Martina Gamil Fayez

**Investigator Title:** principle investigator

**Type:** PRINCIPAL_INVESTIGATOR

### Oversight Module

**Is FDA Regulated Device:** False

**Is FDA Regulated Drug:** False

### Description Module

**Brief Summary:** Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.

### Conditions Module

**Conditions:**

- Pseudomonas Aeruginosa Infection

### Design Module

#### Design Info

**Observational Model:** COHORT

**Time Perspective:** CROSS_SECTIONAL

#### Enrollment Info

**Count:** 100

**Type:** ESTIMATED

**Study Type:** OBSERVATIONAL

### Arms Interventions Module

#### Arm Group 1

**Description:** Pseudomonas Aeruginosa Isolates

**Label:** group1

### Outcomes Module

#### Primary Outcomes

**Description:** Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions

**Measure:** Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections.

**Time Frame:** 6 months

**Description:** Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines

**Measure:** Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas

**Time Frame:** 6 months

**Description:** Detection of biofilm forming isolates by tissue culture plate method.

**Measure:** Detection of biofilm forming isolates

**Time Frame:** 6 months

**Description:** Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction

**Measure:** Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.

**Time Frame:** 6 months

### Eligibility Module

**Eligibility Criteria:** Inclusion Criteria:

* Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

Exclusion Criteria:

* Any infection before 48 hours of patient admission at Hospital

**Maximum Age:** 80 Years

**Minimum Age:** 1 Year

**Sampling Method:** NON_PROBABILITY_SAMPLE

**Sex:** ALL

**Standard Ages:**

- CHILD

- ADULT

- OLDER_ADULT

**Study Population:** Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

### Contacts Locations Module

#### Central Contacts

**Contact 1:**

**Email:** martina.gameel@med.sohag.edu.eg

**Name:** Martina Gamil Fayez, Demonstrator at Microbiology

**Phone:** 01272602453

**Role:** CONTACT

#### Locations

**Location 1:**

**City:** Sohag

**Contacts:**

***Contact 1:***

- **Name:** martina

- **Role:** CONTACT

**Country:** Egypt

**Facility:** Faculty of medicine Sohag university hospital

**Status:** RECRUITING

### IPD Sharing Statement Module

**Info Types:**

- STUDY_PROTOCOL

**IPD Sharing:** YES

## Derived Section

### Condition Browse Module - Ancestors

- ID: D000016905

- Term: Gram-Negative Bacterial Infections

- ID: D000001424

- Term: Bacterial Infections

- ID: D000001423

- Term: Bacterial Infections and Mycoses

- ID: D000007239

- Term: Infections

### Condition Browse Module - Browse Branches

- Abbrev: BC01

- Name: Infections

- Abbrev: All

- Name: All Conditions

- Abbrev: BC23

- Name: Symptoms and General Pathology

### Condition Browse Module - Browse Leaves

- ID: M10283

- Name: Infections

- Relevance: LOW

- As Found: Unknown

- ID: M6368

- Name: Communicable Diseases

- Relevance: LOW

- As Found: Unknown

- ID: M14409

- Name: Pseudomonas Infections

- Relevance: HIGH

- As Found: Pseudomonas

- ID: M4722

- Name: Bacterial Infections

- Relevance: LOW

- As Found: Unknown

- ID: M19249

- Name: Gram-Negative Bacterial Infections

- Relevance: LOW

- As Found: Unknown

- ID: M12136

- Name: Mycoses

- Relevance: LOW

- As Found: Unknown

- ID: M4721

- Name: Bacterial Infections and Mycoses

- Relevance: LOW

- As Found: Unknown

### Condition Browse Module - Meshes

- ID: D000011552

- Term: Pseudomonas Infections

### Misc Info Module

- Version Holder: 2024-05-31
