Solubility of Omeprazole Sulfide in Different Solvents at the Range of 280.35–319.65 K
Solubility data were measured for omeprazole sulfide in ethanol, 95 mass-% ethanol, ethyl acetate, isopropanol, methanol, acetone, n-butanol and n-propanol in the temperature range from 280.35 to 319.65 K by employing the gravimetric method. The solubilities increase with temperature and they are in good agreement with the calculated solubility of the modified Apelblat equation and the λh equation. The experimental solubility and correlation equation in this work can be used as essential data and model in the purification process of omeprazole sulfide. The thermodynamic properties of the solution process, including the Gibbs energy, enthalpy, and entropy were calculated using the van’t Hoff equation.
<!>Introduction<!>Materials<!>Methods<!><!>Solubility Data and Correlation Models<!><!>Solubility Data and Correlation Models<!><!>Solubility Data and Correlation Models<!>Thermodynamic Properties for the Solution<!><!>Thermodynamic Properties for the Solution<!><!>Thermodynamic Properties for the Solution<!>Conclusions
<p>Chemical structure of omeprazole sulfide</p><!><p>Omeprazole sulfide is synthesized using 2-mercapto-5-methoxybenzene imidazole and 2-chloromethyl-4-methoxy-3,5-lutidine [8] or 4-methoxy-3,5-dimethyl-2-pyridine-methyl bromide [9] as substrate. It should be purified by dissolution, crystallization and separation. Crystallization processes are the critical steps that determine the quality of the product [10] of omeprazole sulfide to provide sufficient purity for the next reaction. So it is very important to know the solubility of omeprazole sulfide as a function of temperature and solvent composition in selected solvents required for the preparation and purification of the products [11]. Moreover, no literature study has reported the correlation between the solubility and temperature or the solvent composition. The most basic information for solving the solvent selection problem is the basic physical properties and solubility data [12]. Therefore we measured and correlated the solubility data of omeprazole sulfide in different solvents (ethanol, 95 mass-% ethanol, ethyl acetate, isopropanol, methanol, acetone, n-butanol and n-propanol) over the temperature range 280.35–319.65 K under atmospheric pressure by the gravimetric method [13, 14]. Thus, systematic and necessary information on the crystallization of omeprazole sulfide was obtained. For predicting the solubility of a solute in different solvents, several methods have been presented [11, 15]. This study used the modified Apelblat equation and the Buchowski–Ksiazaczak λh equation to correlate and predict the solubility of omeprazole sulfide in different solvents.</p><!><p>A white crystalline powder of omeprazole sulfide was supplied by Shanghai Lingfeng Chemical Reagent Co., China. The mass fraction was higher than 0.995, measured by high performance liquid chromatography (HPLC type DIONEX P680 DIONEX Technologies). The melting temperature was 392.15 K determined by differential scanning calorimeter (Netzsch DSC 204). The ethanol, ethyl acetate, isopropanol, methanol, acetone, n-butanol and n-propanol used for experiment were all analytical purity grade with mass fraction purity higher than 0.995 except for 95 % ethanol. They were supplied by Shanghai Shenbo Chemical Co., Ltd. and used without further purification.</p><!><p>The solubility of omeprazole sulfide was determined by a gravimetric method. The balance used in these experiments was an analytical balance with an uncertainty of ±0.0001 g (Sartorius, BS210S). 8 mL of solvent and a rotor were put into a 10 mL glass test tube with stopper, and then excess omeprazole sulfide was added into the glass test tube [16]. The test tubes were partly immersed in a constant-temperature bath. The temperature was controlled by a jacketed vessel with water circulated through the outer jacket from a super thermostatic water-circulator bath (type DC-2006 Ningbo XinYi Biotechnology Co., Ltd.). Meanwhile, the inner chamber of the vessel needs a mercury-in-glass thermometer with an uncertainty of ±0.05 K (calibrated by using a standard thermometer) for measuring the solution equilibrium temperature. Continuous stirring was adopted for fully mixing the suspension with a magnetic stirrer at each temperature [17].</p><p>In order to make sure that the solution system established the solid–liquid equilibrium, a stirring time of 12 h was provided, and then the solution was kept still about 3 h to ensure a dynamic balance was established between the dissolution and the crystallization processes. Then, about 1 mL of supernatant was taken from the test tube and transferred to a previously weighed 5 mL sampling vial using a pre-warmed pipette [17, 18] carefully and quickly. Subsequently, the mass of the sample was determined by weighing the sampling vial again. Then the sampling vial was put into a dryer to dry at room temperature. Afterwards the sampling vial was weighed on a regular basis until it reached a constant weight. Each experiment was repeated at least twice to check the repeatability of the solubility determination, and three samples were taken for each solvent at each temperature [19] and the mean value was considered as the solubility. The saturated mole fraction solubility (x) is obtained from the following equation:1\documentclass[12pt]{minimal}
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				egin{document}$$ x = rac{{{{m_{1} } \mathord{\left/ {phantom {{m_{1} } {M_{1} }}} 
ight. \kern-0pt} {M_{1} }}}}{{{{m_{1} } \mathord{\left/ {phantom {{m_{1} } {M_{1} }}} 
ight. \kern-0pt} {M_{1} }} + {{m_{2} } \mathord{\left/ {phantom {{m_{2} } {M_{2} }}} 
ight. \kern-0pt} {M_{2} }}}} $$\end{document}x=m1m1M1M1m1m1M1M1+m2m2M2M2where m
1 represents the mass of solute and m
2 the mass of solvents. M
1 is the molecular mass of solute and M
2 that of the solvent.</p><!><p>The saturated mole fraction solubility (x) and the calculated solubility values (x
c) by Eqs. 2 and 3 of omeprazole sulfide, in different solvents at the temperature range from 280.35 to 319.65 K</p><!><p>The relationship between temperature and mole fraction solubility in different solvents is described by the modified Apelblat equation, which is a semiempirical equation derived from the Clausius–Clapeyron equation [20, 21], which is as follows:2\documentclass[12pt]{minimal}
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				egin{document}$$ \ln \, x = A + rac{B}{T} + C\ln T $$\end{document}lnx=A+BT+ClnTwhere T represents the absolute temperature, A, B and C are the model parameters, and x is the mole fraction solubility of omeprazole sulfide. The constants A and B represent the variation in the solution activity coefficient and provide an indication of the effect of non-ideal solution behavior on the solute solubility, while the constant C reflects the temperature influence on the enthalpy of fusion [22]. The adjustable parameters A, B and C can be obtained by fitting the experimental solubility data.</p><p>The Buchowski–Ksiazaczak λh equation is an alternate way to describe solid–liquid equilibrium behavior of omeprazole sulfide, as first proposed by Buchowski et al. [23]. The experimental data for many systems can be well represented by the Buchowski–Ksiazaczak λh equation with only two parameters λ and h [24–27]. In this paper, the solubility data were also correlated with the Buchowski–Ksiazaczak λh equation:3\documentclass[12pt]{minimal}
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				egin{document}$$ \ln \left[ {1 + rac{\lambda (1 - x)}{x}} 
ight] = \lambda h\left[ {rac{1}{T} - rac{1}{{T_{m} }}} 
ight] $$\end{document}ln1+Î»(1-x)x=Î»h1T-1Tmwhere T represents the system temperature, T
m is the melting point temperature of omeprazole sulfide in Kelvin, x is the mole fraction solubility of omeprazole sulfide and λ and h are the model parameters determined by the experimental data in the system.</p><!><p>Parameters of Eq. 2 for mole fraction solubility of omeprazole sulfide in various solvents</p><p>Parameters of Eq. 3 for mole fraction solubility of omeprazole sulfide in various solvents</p><!><p>The RMSD is defined as follows:4\documentclass[12pt]{minimal}
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				egin{document}$$ {	ext{RMSD}} = \sqrt {rac{{\sum
olimits_{i = 1}^{N}
{(x^{	ext{e}} - x^{	ext{c}} )^{2} } }}{N}} $$\end{document}RMSD=∑i=1N(xe-xc)2N</p><p>The RAD is defined as follows:5\documentclass[12pt]{minimal}
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				egin{document}$$ {	ext{RAD}} = rac{1}{N}\sum\limits_{i = 1}^{N} {\left|
{rac{{x^{	ext{e}} - x^{	ext{c}} }}{{x^{	ext{e}} }}} 
ight|}
$$\end{document}RAD=1N∑i=1Nxe-xcxewhere N is the number of experimental points obtained in each set, which equals the number of temperatures used, x
c represents the calculated solubility values and x
e the experimental solubilities.</p><p>The relative deviations (RDs) between the experimental values and the calculated values are also presented in Table 1. The RDs are given as:6\documentclass[12pt]{minimal}
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				egin{document}$$ {	ext{RD}} = rac{{x^{	ext{e}} - x^{	ext{c}}
}}{{x^{	ext{e}} }} $$\end{document}RD=xe-xcxewhere x
c represents the calculated solubilities and x
e the experimental values.</p><!><p>Solubilities of omeprazole sulfide in different solvents at atmospheric pressure. Left pointing filled triangle methanol, filled square ethanol, filled triangle 95 % ethanol, open square
n-butanol, inverted triangle
n-propanol, open circle acetone, right pointing filled triangle isopropanol, filled circle ethyl acetate. Solid lines calculated using Eq. 2</p><!><p>As we all know, methanol, ethanol, propanol and butanol are protic solvents that have a hydrogen atom bound to an oxygen (in a hydroxyl group). The molecules of such solvents can readily donate protons and interact with solute molecules by hydrogen bonding. The hydrogen bonds could increase the solubility of the solute. This may be the reason that the solubility is relatively high in methanol, ethanol, 95 % ethanol, n-butanol and n-propanol. The solubility in methanol and ethanol is higher than that in n-propanol and n-butanol. This phenomenon can be explained by the fact that when the alcohol chain length increases, the hydrogen bonds with alcohols are weakened [29]. However, the solubility in isopropanol is relatively low, perhaps because the hydroxy of the isopropanol molecules is located between two CH3 groups, which hinder the interaction of the H and N atoms. The solubility of omeprazole sulfide is lower in 95 % ethanol than in ethanol. The reason may be that the sulfide compounds are practically insoluble in water.</p><p>As can be seen from Tables 1, 2 and 3, the calculated data of omeprazole sulfide in a total of eight solvents show good agreement with the experimental data. For the modified Apelblat equation, as we can see from Tables 1 and 2, the RADs are 0.97, 0.41, 0.95, 0.87, 0.93, 0.49, 0.46 and 0.56 %, respectively and the absolute values of RDs do not exceed 2.6 %, which indicates that all the solubility data can be calculated in the selected solvents when the modified Apelblat equation is used. The same is true for analyzing the solubility data and the parameters that fitted the λh equation. Furthermore, from Tables 2 and 3, all the solubilities are calculated with reasonable RMSD and the average RMSDs are 0.85 and 1.2, for the modified Apelblat and Buchwski–Ksiazaczak λh equations respectively. Therefore the regression result of the modified Apelblat equation is more accurate than the Buchowski–Ksiazaczak λh equation. Compared to the Buchowski–Ksiazaczak λh equation, the modified Apelblat equation is proposed for solid–liquid equilibria, and it is widely accepted as being capable of dealing with solvent systems. Therefore, the measured solubility data and the correlation equation in this work can be applied to the design and optimization for the extraction and purification process of omeprazole sulfide [19].</p><!><p>The temperature dependence of the solubility allows a thermodynamic analysis that permits insight into the molecular mechanisms involved in the solution processes [30]. In this study, the thermodynamic functions in the process of solution of omeprazole sulfide are calculated on the basis of the solubility of omeprazole sulfide in different solvents. The standard molar enthalpy of solution \documentclass[12pt]{minimal}
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				egin{document}$$ \left( {pdelta H_{	ext{soln}}^{	ext{o}} } 
ight) $$\end{document}ΔHsolno is accessible from this equation, which is the van't Hoff analysis and defined as [30–32]:7\documentclass[12pt]{minimal}
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				egin{document}$$ pdelta H_{	ext{soln}}^{	ext{o}} = - R\left( {rac{{\partial \ln x_{1} }}{{\partial \left( {{1 \mathord{\left/ {phantom {1 T}} 
ight. \kern-0pt} T}} 
ight)}}} 
ight) $$\end{document}ΔHsolno=-R∂lnx1∂11TTwhere x
1 is the mole fraction solubility, R represents the universal gas constant (8.314 J·K−1·mol−1) and T is the absolute temperature. The standard molar enthalpy change of solution, \documentclass[12pt]{minimal}
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				egin{document}$$ pdelta H_{	ext{soln}}^{	ext{o}} $$\end{document}ΔHsolno, is generally obtained from the slope of the solubility curve in a so-called van't Hoff plot where ln x is plotted against T
−1. Over a limited temperature interval, the heat capacity change of a solution may be assumed to be constant, hence the derived values of \documentclass[12pt]{minimal}
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				egin{document}$$ pdelta H_{	ext{soln}}^{	ext{o}} $$\end{document}ΔHsolno will also be valid for the mean temperature, T
mean = 300 K [33]. Equation 7 can also be written as:8\documentclass[12pt]{minimal}
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				egin{document}$$ pdelta H_{	ext{soln}}^{	ext{o}} = - R\left( {rac{\partial \ln x}{{\partial \left( {{1 \mathord{\left/ {phantom {1 T}} 
ight. \kern-0pt} T} - {1 \mathord{\left/ {phantom {1 {T_{	ext{mean}} }}} 
ight. \kern-0pt} {T_{	ext{mean}} }}} 
ight)}}} 
ight) $$\end{document}ΔHsolno=-R∂lnx∂11TT-11TmeanTmean</p><!><p>Mole fraction solubility (ln x) of omeprazole sulfide in different solvents against 104 (1/T − 1/T
mean) with a straight line to correlate the data. Left pointing filled triangle methanol, filled square ethanol, filled circle 95 % ethanol, diamond
n-butanol, open square
n-propanol, right pointing filled triangle acetone, inverted triangle isopropanol, filled triangle ethyl acetate</p><!><p>The standard molar Gibbs energy of solution \documentclass[12pt]{minimal}
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				egin{document}$$ pdelta {	ext{G}}_{	ext{soln}}^{0} $$\end{document}ΔGsoln0 can be calculated according to [34]:9\documentclass[12pt]{minimal}
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				egin{document}$$ pdelta G_{	ext{soln}}^{	ext{o}} = - RT_{	ext{mean}} 	imes {	ext{intercept}} $$\end{document}ΔGsolno=-RTmean×interceptwhere the intercept used is that obtained in plots of lnx versus (1/T − 1/T
mean). The standard molar entropy of solution is obtained from [30]:10\documentclass[12pt]{minimal}
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				egin{document}$$ pdelta S_{	ext{soln}}^{	ext{o}} = rac{{pdelta H_{	ext{soln}}^{	ext{o}} - pdelta G_{	ext{soln}}^{	ext{o}} }}{{T_{	ext{mean}} }} $$\end{document}ΔSsolno=ΔHsolno-ΔGsolnoTmean</p><!><p>Thermodynamic functions relative to solution process of omeprazole sulfide in solvents at mean temperature</p><!><p>The conclusion can be drawn from Table 4 that the enthalpy and the standard Gibbs energy of solution of omeprazole sulfide are positive in the eight solvents, indicating the solution process of omeprazole sulfide in a total of eight solvents is endothermic. Moreover, the main contributor to the standard molar Gibbs energy of solution is the enthalpy during the dissolution, because the values of % ξ
H are ≥54 %.</p><!><p>The solubility of omeprazole sulfide in a total of eight solvents has been measured from 280.35 to 319.65 K by a dependable experimental method and simple solubility apparatus. For all solvents, solubility is a function of temperature and increases with increasing temperature, but to each increment of temperature they responded with a definite change of solubility. The experimental data were fitted by using the modified Apelblat equation and λh equations and the Apelblat equation is more accurate than the λh equation for this system. The calculated solubility of omeprazole sulfide shows good agreement with the experimental values, and experimental solubility data from this work can be used for designing a purification process of omeprazole sulfide. The thermodynamic properties for the solution process including Gibbs energy, enthalpy, and entropy were obtained by the van't Hoff analysis. The thermodynamic parameters values show that the solution process of omeprazole sulfide in a total of eight solvents is endothermic and the larger contributor to the standard molar Gibbs energy of solution is the enthalpy change during the dissolution.</p>