How to test image segmentation algorithms? What would be an ideal environment/manner to judge the effectiveness of multiple segmentation algorithms? The goal is to determine which algorithms provide the best results for pre-clinical imaging. Our group has considered a GUI in MATLAB, and have different modules for different algorithms. Various papers have conducted such algorithm analyses in clinical scenarios, but none give an indication to their environment.
Google "image segmentation benchmark".  You will find various data sets with ground truth, such as this one.