Finding differentially expressed genes between two samples Hi, I was wondering whether we can find the differentially expressed genes between the Double-KO and the Shox2-KO. For the FindMarker() function, only see the differential expressed gene between different identities. I don't know whether we can find the differential expressed gene between only these two samples.
If I understand your question correctly, you can simply use SetIdent() to change the "default identity" to your samples and then use FindMarker() with the ident.1 = "Double-KO" and ident.2 = "Shox2-KO".

Alternatively, you can "collapse" or "summarize" your single cell data into a pseudo-bulk and then use one of the bulk RNA-seq packages.