Finding orthologues using BLAST on the NCBI database I'm an informatics student who has essentially zero knowledge of biology. I BLASTed my gene and have 1000s of results with very low E values. Where do I go from here if I want to find orthologues?
BLAST is often a sensible way to find gene/protein homologs, but resolving those as orthologs vs paralogs etc. is a non-trivial task, especially when considering large numbers of species.

Since you are new to bioinformatics I recommend you search an ortholog database that already exists.

Here are some databases:


orthoDB  
OMA  
EggNog


All of these should allow you to search either with text or a sequence to identify the orthologous groups that your input belongs to at different taxonomic levels. Sequence searches either follow pairwise alignment (e.g. BLAST, DIAMOND, RAPSEARCH) or profile HMM type (e.g. HMMER3) approaches.

A more complete list of ortholog databases is available from "Quest for orthologs".