tool for the prediction of impact of mutations on protein localization I need to know if the mutations present in the "position of interest" can have an impact on the protein localization ability. Is there any tool to make such prediction that can make my life easier? 

I know the position of nuclear localization sequence in a protein and I also know it has a mutation, but I don't know how to predict the impact of this mutation on the localization of this protein ie if this mutation hindering protein's entry to the nucleus or not? Should an analysis of protein stability be useful it this case, as an alternative?

I am not interested in localization itself but the impact of mutations on the localization.
Do you have the protein sequence after the mutation available with you?
If so then maybe you can BLAST them and find out where the best hits are localized using GO annotation (cellular components). I am aware that these won't be your exact protein sequence but they can at least give you a rough idea.