Why does Coronavirus genome contain poly A region at the 3' end in GenBank? If you look at the Coronavirus genome below, it contains poly-A region at the end. I know that the viral RNA contains a poly-A tail. But, the genome in GenBank is stored in cDNA format. That means the end should have poly-T instead of A. Can anybody help me to understand this, please ?
&gt;MN988713.1 Wuhan seafood market pneumonia virus isolate 2019-nCoV/USA-IL1/2020, complete genome
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAA
--------------------------------------------------------------------
GATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAAT
TTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAAAA
I figured out that it has to do with second strand of cDNA. Usually genbank sequences are equivalent to 5’ to 3’ strand of cDNA. This strand is generated using second strand synthesis enzyme.