How many reads has my sequencing run produced on minion? I am running a 48 h sequencing protocol on a FLO-MIN107, on MinION, with DNA library-prepped with SQK-RAD004. MinKNOW is installed on my Mac and controlling the MinION. I have found the following histogram in MinKNOW. What do the numbers mean? In particular, how many reads have been produced? I understood that the events are consecutive electrical signals and are then transformed into reads? Do I have 4029 reads? The run has started 24 hours ago. 4029 reads surely is really low, isn't it? What does 4,120,722 mean?



The Platform QC of the flow cell was as follows: 


group 1: 480
group 2: 374
group 3: 201
group 4: 59
total = 1114
That's indeed the number of reads and that's quite low. How was your pore occupancy (number of pores sequencing) and your flow cell QC (number of pores good enough for sequencing)? How was your library concentration?

Events is an approximation of nucleotides: it's a guess based on the current signal. As far as I know if you multiply this by 1.8 you get approximately your throughput in nucleotides.

The 4,120,722 is the number of events in the currently selected (mouse hovering?) bin of the histogram.