Reading multiple raw files in Seurat I have multiple single cell samples to analyze and I'm following the instructions in Satija Lab's website. I want to merge all the count files from all the samples at once, and associate the metadata to each sample. 

For the Read10x command, is there a way to read multiple files at once, such as merging all the filter_featire_bc_matrix files?

I have all the samples saved in a directory on my desktop, is it possible to call the directory within the script for it to read all the raw counts?

This is what I have thus far:

# load the required libraries
library(dplyr)
library(Seurat) 
library(ggplot2)

-----------------------------------------------

# set up the working directory

wd = ("~/Seurat") setwd(wd)

# read all metadata

metadata = read.table("metadata_Jan2020.csv", sep=';', header=T, stringsAsFactors=F)
rownames(metadata) = metadata$LibraryID

#metadata
#mf = metadata

mf = metadata %&gt;% filter(Type %in% c("PDAC", "NORMAL"))
mf = mf %&gt;% filter(!(ID %in% c("PDAC-HTB2854")))`
Seurat has a vignette specifically for combining multiple 10x libraries.