How to know which ortholog database is the best for my work? I am working on four data sets of yeast genes and one data set of mouse gene. I need the list of human orthologs of those genes. There are a lot of databases available online which is in use for this purpose
https://questfororthologs.org/orthology_databases
I don't know which one to choose and why. Can anyone tell me on what basis should I choose a database over any other database? Thank you.
To recover orthologous sequence for model species
You should be able to find your orthologs using BioMart and your gene IDs.
There are numerous way to do it, directly on the ensembl website or through API. You can start from these links (quite old though) :

http://www.ensembl.info/2009/01/21/how-to-get-all-the-orthologous-genes-between-two-species/
https://www.youtube.com/watch?v=EwfOQsr2O-M #Get orthologs via BioMart
https://www.biostars.org/p/12245/ #Finding Human/Yeast Orthologs Using Embl?

Which database should I use
It is broad and depends on the nature of your work. In your case, working with &quot;major model species&quot;, DIOPT (the first line of the table in Quest For Orthology) has the very feature you need on its front page for going from mouse to human. More eukaryotic/viral oriented database will be used for taxonomic identification, annotation inference, etc.
I hope it helps.