Can we use yx5300 to play audio from a file in Arduino? I want to play a file that I have in my USB device through a yx5300 mp3 module.

I have a file in my USB device at path Music/hello.mp3. I want to play this file using the yx5300 module or some other module, if available.

I have already read this file using a shield. I want to pass the data to the yx5300.
No. That chip can only play files on an SD card that is directly attached to it.