Reference an UTXO of a transaction that has not been yet mined How to reference an UTXO that has not been yet mined? I want to prepare a transaction and, before spreading it, use its UTXO as input for another relative timelocked transaction. Then, spread the first prepared transaction.
How to do it?
The UTXO is referenced as an outpoint(TXID, Index) in the input of your time-locked spending transaction.

So, when creating your time-locked transaction, you need to know the TXID of the (unconfirmed) transaction you are spending. 

Simply double+sha256 and swap endianness of the over-the-wire transaction serialisation to get the TXID you wish to reference. 

This TXID is a unique identifier (assuming no TX malleability). The outpoint you are referencing in your spending transaction is not affected by the confirmation of said UTXO.