Launching a python script from an insert trigger
Don't make your user transaction wait for the (hopefully!) successful completion of the Python script. Your entire transaction sits there and waits for this external process to run, try to send mail, etc. I doubt the e-mail really has to go out that instant - especially given you can't control any delays it has as it gets routed to the recipient's inbox anyway. Why not just run the process more frequently, if timing is so important?

Please give this tip a look-through.

If you really, really, really want to do this the wrong way, you can just enable xp_cmdshell and fire away.

EXEC sp_configure 'show advanced options', 1;
GO
RECONFIGURE WITH OVERRIDE;
GO
EXEC sp_configure 'xp_cmdshell', 1;
GO
RECONFIGURE WITH OVERRIDE;
GO


Now, assuming the user has access to xp_cmdshell and/or the SQL Server service account can see the folder where the python script is stored, you should be able to do this from within your trigger:

EXEC master..xp_cmdshell N'C:\Python27\python.exe C:\source\NotifyAgents.py';


As an aside, you should state in your question that you aware that this is a very bad thingTM, but you are not concerned with that, for whatever reason. I still don't think you're going to get as real time as you expect, even if you do fire this from the trigger. Have you considered database mail instead of python?