${\bf NP} \not = {\bf E}$ and ${\bf PSPACE} \not = {\bf E}$
${\bf E} \not = {\bf NP}$ does not imply ${\bf E} \subset {\bf NP}$ nor ${\bf NP} \subset {\bf E}$. Similarly, ${\bf E} \not = {\bf PSPACE}$ does not imply ${\bf E} \subset {\bf PSPACE}$ nor ${\bf PSPACE} \subset {\bf E}$. You would need to show one of these containment's to be able to get a proper separation result out of those inequalities.