Big O relation between $2^n$ and $2^{2n}$
The more strong estimate is correct:

$2^n = o(2^{2n})$

Just look at definition of the little-o. For any constant $c \gt 0$ you need to find a constant $n_0$, such that for all $n \ge n_0$ you get $2^n \le c \cdot 2^{2n}$. Apparently, the following inequality will give you this $n_0$:

$n_0 \ge ln(1 / c)$