Proving that the set of deciders is not Turing-recognizable
The basic idea of the proof is to come up with a Turing machine which doesn't belong to the enumeration, hence contradicting the claim that deciders can be enumerated. To this end, we use diagonalization – for each potential machine in the enumerate, we ensure that there is at least one input on which the diagonalizing machine has a different behavior.

If the diagonalizing machine $D$ has alphabet $\Sigma$, then we already know that any machine in the enumeration with a different alphabet cannot be $D$. Therefore $D$ doesn't care much about these machines. What $D$ has to ensure is that for every machine $M_i$ with alphabet $\Sigma$ there is an input $x_i$ such that $D(x_i) \neq M_i(x_i)$.