What encryption to choose from the SSL library in python 3?
You may need a 1.2 and 1.3 scheme, so I'd include at least one of both, giving priority to 1.3 as it is the latest / greatest protocol.

Then there is ECDSA and RSA, indicating the certificate based authentication used. ECDSA is more performant for similar security (and you may not even get any certificates above RSA-4096). That may be important if you can choose the certificate type. However, once you've chosen your TLS certificate type, you don't get to choose between the two cipher-suites, and you just include the one that is compatible with your certificate.

If you do Business-to-Business (B2B) then I'd choose TLS 1.3 due to the fast session resumption may achieve.