Finding the cause of a tubless loss of sealant
If the tape has a hole in it, that's definitely the problem. I'm not sure why the tape would suddenly fail as there is nothing rubbing in it, although the spoke hole in the rim looks strange to me. Is there a sharp edge there that punctured the tape?

If the tire had a large leak you be able to see sealant coming out of it, same if there were a problem with the bead seating on the rim. If you have not seen this your tire is probably OK.

You'll need to replace the tape, perhaps with a heavier duty type. If you can't seat the tire bead yourself enlist the help of a bike repair shop - that's what they're for: backup when you don't have the tools or knowledge to do it yourself.

Methylated spirits or isopropyl alcohol works for removing dried sealant.