Can Rikudou Madara activate his Limbo Perfect Susanoo in the anime after Mugen Tsukuyomi ends?
He never does, but he theoretically could. He's still an Uchiha, and still has the Rinnegan and also uses Limbo while in Six Paths mode, however there is no reason for him to. The Truth Seeking Balls are a more perfect defense than Susano'o and the new abilities granted to him by the Six Paths' chakra seemed to be at least as powerful, if not moreso, than his Susano'o.