How to calculate extent of Sequence similarity
Well. You perhaps need not use the bioinformatic algorithms of string matching. These algorithms were primarily developed because of the extensive search space. 

In your problem you just need to compare two recording experiments and you should use mathematical metrics for it. You have a 60x4 i.e 240 dimensional random variable. Moreover this variable is not discrete, it is continuous. For similarity measurements you have to use statistical tests or otherwise define a cutoff either on each element of the vector or the total magnitude defined by any norm.