Writing methods section on PCR amplication in a paper
Here's my take, just a few minor changes which are shown in bold (I have also changed ,to . for decimal numbers and added a few spaces where needed):


  PCR Amplification and sequencing
  
  A PCR reaction for sequencing was performed using universal primers ITS5: F:
  (5’TCCTCCGCTTATTGATATGC3’) and ITS4:R: (5'TCCGTAGGTGAACCTGCG C3'),
  including 5.8 S rRNA region (White et al. 1990). Amplification condition
  was performed in a 25 ml reaction volume and consisting of 10 µL
  nuclease free water, 12.5 µL Taq green master mix, 0.5 µL forward
  primer, 0.5 µL reverse primer, 0.5 µL DMSO, 1µL template. PCR was performed using a Biorad PCR machine. The thermal cycle was
  programmed for 90 s at 95 °C for initial denaturation, followed by
  35 cycles of 30 s at 95 °C for denaturation, 30 s at 55 °C for
  annealing, 90 s at 72 °C for extension, and 5 min at 72 °C for the final extension.
  PCR products were examined by electrophoresis at 100 V for
  30 min in a 1% (w/v) agarose gel in 1 x TAE buffer. The marker
  used a 1 kb DNA ladder. The electrophoresis gel was soaked in ethidium
  bromide for 30 min then examined in UV light.