Do mitochondria use ATP in order make another ATP from glucose?
During ATP production through the citric acid cycle, some ATP is used to transport necessary molecules for the reactions.


piruvate molecules are actively transported into the mitochondrial matrix [2]
two NADH equivalents from glycolisis are transported into the mitochondrion and consume two molar equivalents ATP [1]
protons leakage across the mitochondrial membrane leads to reduced ATP from NADH and FADH2 [1]


In theory, one molar equivalent of glucose can generate 38 equivalents of ATP. The actual number is only 25 [1].



References: 


Wikipedia contributors, "Citric acid cycle," Wikipedia, The Free Encyclopedia, http://en.wikipedia.org/w/index.php?title=Citric_acid_cycle&amp;oldid=610117265 (accessed June 26, 2014).
Wikipedia contributors, "Mitochondrion," Wikipedia, The Free Encyclopedia, http://en.wikipedia.org/w/index.php?title=Mitochondrion&amp;oldid=614223751 (accessed June 26, 2014).