how to culture mc38 cells
Edit: Similar questions have been asked before: Textbook/lab manual for mammalian cell culture
Cell culture reagent/consumables suppliers can have good step by step recommendations for how to use their products. For example Thermo has some guidelines that you may find helpful. Specifically, cell culture maintenance would seem to be helpful.
These folks also have what superficially look like good resources.
Reviewing MC38 culture requirements is also always a good idea.
Good luck!