Kinesin-5 / cytoplasmic dynein spatial density distribution in neurons
It is possible to tag molecular motors with fluorescent proteins. It may impede with its movement but as this paper describes, a variant can be created that doesn't have cargo binding ability. 


  Constitutively active kinesin motors can be generated by truncations
  that remove autoinhibitory and cargo-binding regions of the
  polypeptide. For this work, we generated KHC(1-560) (Figure 1A), a
  dimeric motor that has been well characterized in vitro and in vivo
  [16],[18],[19]. KHC(1-560) motors were tagged with three tandem copies
  of monomeric Citrine (mCit), a variant of enhanced yellow fluorescent
  protein (FP) (Figure 1A), and expressed in COS cells (Figure 1B).
  Single Kinesin-1 motors were tracked in live cells using a modified
  TIRF microscope (Figure 1C) in which the angle of illumination was
  varied to enable deeper imaging as described [17]


However, if your objective is just to determine the density distribution of Kinesin and Dynein in a Neuron, you can simply fix the cells and do IHC staining for the motor proteins followed by high resolution imaging.