Dilution question about RNA
If I understand correctly what you mean by "I would like to dilute my RNA to 100 ug":

Your RNA preparation is at 3.3 &mu;g &mu;l-1.

To add 100 &mu;g to a reaction you need to add 100/3.3 = 30 &mu;l

Then make the total volume up to 1000 &mu;l with enzyme and buffer as appropriate.