Machine learning for light microscopy -- problems to solve?
I know this question is going to close. But, if you want to work something you can work on: 

Cryo super-resolution fluorescence imaging


  Highlights
  
  
  CryoFM allows imaging of vitrified biological samples with fluorescence microscopy.
  There are significant challenges to achieve high-resolution cryoFM imaging.
  Fluorophore characteristics at low temperature offer additional advantages.
  Cryo super-resolution fluorescence imaging will give dramatic resolution improvement.
  


Source: Fluorescence cryo-microscopy: current challenges and prospects.