How to design a permanent vaccine to cholera using CRISPR?
So, would it be possible to engineer a CRISPR gene editing tool to
  prevent Vibrio Cholerae from getting into our cells?


Vibrio Cholerae doesn't get into our cells.

Did you observe that your paper has no in vitro experiments?

It's going to be tough to get a cell to transcribe and translate something so small, and will the peptide work if it needs a leader sequence to be exported out of the cell?

Its much much easier to break a gene in every cell than to add a new gene and have just a small subset of cell types make large quantities of that gene product.