What conditions should I use for Gel Red staining?
I have had success with 1.5% agarose gels in TBE pre-stained with gelred.  I use a 10,000x stock solution of gelred sold by http://biotium.com.  I visualize the gel on an old fotodyne UV transilluminator.

After melting agarose in the appropriate volume of TBE in a microwave, I let it cool to about 50 C (I can touch the flask with the back of my hand without snatching it away) and then add the appropriate volume of stock gelred.  

For a 30 mL 1.5% agarose gel:


mix 0.45g agarose + 30 mL 1x TBE
microwave until agarose solution boils and clarifies
let cool to around 50 C
add 3 uL gelred
swirl the mixture
cast the gel (don't forget comb)


This typically works for me.  I run the gel in a 1x TBE buffer at around 120 volts.