Finding total concentration of enzymes
Hi again TheLast Cipher,
Enzymes in a cell are usually semi-quantified by the: Western Blot technique.
But there are lesser known techniques.
E.g. For a Western Blot the unit of measurement is the color intensity of the enzyme band on the blot membrane.
If I were you I would look for journal articles covering your pathway of interest.
E.g. https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.5520320129
Covers the electron transport chain (Co-enzyme Q10)
If you can get through the pay-wall &amp; read their results and you will find:

&quot;Western blot analysis of membrane protein isolated from keratinocytes derived from one young and old donor. A representative picture out of five experiments is displayed.&quot;

Also they used and I quote:

&quot;Arbitrary Units&quot;

Maybe you could comment on which pathway and I can find some assisting material.