Find the maximum frequency in the signal section?
Is there someway to tweak the signal to obtain the doppler shift ?


Yes.

Your objective is to retrieve the phase shift of the recorded signal due to the Doppler Phenomenon. This is most commonly done with a Phase Detector which integrates the product of two sinusoids.

So, in order to generate a signal that is proportional to the target movement, you can generate a sinusoid at the same frequency as the signal you are sending out of the speakers, multiply it with the received signal and then integrate it over time with a simple integrator.

This will give you a varying DC signal whose amplitude will be proportional to the relative hand movement. This means that in one instance of the experiment, this signal might increase when the hand is moving away from the detector and decrease when the hand is moving towards the detector and in another instance this relationship might appear reversed.

To avoid this problem you are going to have to synchronise the "generated sinusoid" with the received sinusoid.

Eventually, we get to the concept of a Phase Locked Loop (PLL) which is an Automatic Control System that does exactly this job: It synchronises a local oscillator with a received signal so that they co-vary in phase. Due to its operation, the PLL generates an error signal which represents the difference between the phase of the local oscillator and the received signal. 

It is exactly this signal that you are after, which is valid after the PLL has "locked" on to the incoming signal.

It is likely however, that you will need to do some pre-processing to your received signal, especially if it is in the audible range and it is recorded via a microphone. Various noise sources (e.g. your palm, compressing air towards the microphone which will create a low frequency whooping sound, mobile phone ringtones and other sources) will make the job of the PLL difficult and you will have to apply at least a high pass filter (e.g. after 15kHz) to make sure that the effect of these noise sources on the recording is minimised.

Hope this helps.