Subtle (and not-so-subtle) humor in scientific literature
I see humorous titles in scientific articles now and again, like the "Wizard of Odds" joke in a recent commentary in Epidemiology. One should however be somewhat cautious. The general use of "marketing gimmicks" like questions in the title have been suggested to increase downloads but not actual citations - which flawed or not flawed form the basis for how both the paper and you as the author are evaluated.

Consider this finding:


  The results of the current study indicate that in two prestigious scientific journals in psychology the use of exceptionally amusing titles (2 standard deviations above the average rated amusement) was associated with a substantiate ‘penalty’ of around 33% of the total number of citations. The present results were found in both of the examined journals and cannot be attributed to potential moderating effects of the title length and pleasantness, the number of authors, the year of publica- tion, and the article type (regular article vs comment).


While that might not be perfectly generalizable, I think it's pretty easy to say that being overly clever is hazardous.