Research Associate job reposted, shiould I reach out to the PI?
A significant proportion of academic jobs are advertised publicly, but in reality they are created for a pre-selected candidate. It is extremely frustrating that many talented candidates routinely waste time end effort applying for jobs where they will not be shortlisted, even if their skills are a close match to published job specification.  I am sorry to hear you find yourself in this situation. The old boys' club is still strong in academia and it hurts normal candidates a lot.
For an individual like you, it is probably advisable to move on and apply elsewhere. Some places have a more fair assessment process for all applications, and the more you apply the higher is the chance you will be invited for an interview.
Good luck.