Keysight Waveform Building Software
Is there a way to do this with the 33220a, for example telling it to
  not send any signal for 1 second without requiring any samples


Can you use it in some form of single shot mode then use a 1 second pule to repeat the waveform?


  Hi @Andyaka, I looked into that and you can use the burst mode with
  the arbitrary waveform to trigger it only once per second, thanks for
  the advice!