OP Amp non-inverter amplifier - Do I have to use power supply to amplify a signal?
First, get rid of those awful DMM blocks, and put in measurement probes. It's a lot cleaner and easier to look at. As for he question itself, yes, power supplies are required. You can't get a higher voltage without deriving it from some higher voltage. (Actually, you can, but that is beyond the scope of this question.) The fact it works without power connected means that component model is garbage, and that kind of surprises me coming from Multisim.