Adding a DC bias to an AC signal for amplification and measurement
Figure 1. OP's circuit.


You have biased the op-amp input at 2.5 V DC.
You have configured the amplifier as non-inverting with a gain of ten.
Output will try to go to 25 V.
You are powering the amplifier with only 5 V so it has saturated.


I think you are expecting it to amplify the AC but not the DC component.