Interleaving in litz wire transformer - to be or not to be?
It is better to do interleave winding method to reduce the number of consecutive layers with rising MMF proximity losses towards centre of core.

Since your turns ratio is 2:1  this is what your interleave pattern should be 2P1S... repeating.

If Eddy current proximity losses can be minimized, the benefit is lower core temp rise at rated current.

There are many variables with core types , geometry and winding methods and skin depth becomes more important as the number of layers increases.