why the 3 stage differential opamp not worked
The LM741 is a very old OP-Amp. It has no rail-to-rail feature.
So you can easily subtract (add) 2 volts from (to) every rail to get the remaining usable voltage range. If every stage can only use 1 V for amplification, the whole circuit is rather unlikely to work as you may guess.

If you look into the datasheet of the LM741, you can clearly see, that the minimum recommended voltage is +-10 V. Next time, read the datasheet before designing a circuit and check the relevant figures.

Now you may ask, why your simulation does not reflect the OP-amps behaviour? Simply spoken, the OPs behaviour in operating conditions which are outside the recommended values is highly nonlinear and unpredictable. Implementing this behaviour in a simulation model is a very high effort with little benefit.