How to set up Drupal 7 on Apache/2 without .htaccess files ?
You should be able to take the contents of the .htaccess file, and place them in your httpd.conf.  Exactly where depends on how you have your site running in Apache.

If you are using a virtual host, you would place everything inside a &lt;Directory&gt; section that matches your DocumentRoot, inside your &lt;VirtualHost&gt; section for the site.

If you aren't using a virtual host, then everything would (probably) still go in a &lt;Directory&gt; section that matches your DocumentRoot, but I can't remember the last time I didn't use a virtual host, even for servers with a single website on them.

You may get better answers over on ServerFault, as this isn't totally Drupal specific.