Interpreting this plot from GSEA
In Deseq2, it depends on contrast eg. if your contrast &lt;- c(&quot;Condition&quot;, firstC, SecondC) is then -ve is downregulated in FirstC and same for +ve. It is always better to take look at count data for the confirmation (responders versus non-responders samples).
[for more detail you can refer here][1]
[1]https://github.com/amarinderthind/RNA-seq-tutorial-for-gene-differential-expression-analysis/blob/master/RNA-Seq-DGE.R