State of the art in predicting Translation Initiation Sites
Ribosome Profiling experiments profile the positions of ribosome-protected mRNA. a cycloheximide treatment fixes the translating ribosomes. A naive approach to define Translation Initiation Sites (TIS) would be to look at positions where you see distinct 'peaks' in the distribution of these fragments since the ribosome density will be higher at the start of the CDS (at the TIS).

TISDB is a database comprised of GTI-Seq global mapping of TIS codons at nearly single-nucleotide resolution. GTI-Seq uses two 'fixers' that fix ribosomes where one of them specifically fixes the initiating ribosomes.