the model matrix is not full rank : This is a classic question which a biologist face without clear understanding of the model design
&gt; table(doldat$Group, coldat$Mutation)
       
        DNMT3A-R882H FLT3-ITD IDH2-R140Q KRAS-G13D MAU2-Q98H No NPM1-L287ins(TCTG) NRAS-G13D STAG2-R614 TET2-E1357 TET2-R550
  HSC              0        0          0         0         0  7                  0         0          0          0         0
  Blast            2        4          2         1         1  0                  4         1          1          6         1
  CMP              0        0          0         0         0  8                  0         0          0          0         0
  GMP              0        0          0         0         0  7                  0         0          0          0         0
  LSC              2        3          0         0         1  0                  0         1          1          0         0
  Mono             0        0          0         0         0  6                  0         0          0          0         0

Look at this table, you see by eye how some mutations are nested with Group. If you want to include it then you will need to subset the experiment into multiple separate runs, depending on the question you want to answer.