How to calculate RNA copies in qPCR using R?
Now that we've straightened out the data, it seems like the simplest solution is to just linearly interpolate the copy number from your standard curve.
When I read your standard curve (std) and your sample data (ct) into R, I see that the log10(Ct) fits the copy number fairly well, whereas the linear is a bit harder:

So we can fit a model to predict copy number based on Ct on the STD data:
&gt; fit = lm(std$Log10.STD_copies. ~ std$STD_Ct)
&gt; fit

Call:
lm(formula = std$Log10.STD_copies. ~ std$STD_Ct)

Coefficients:
(Intercept)   std$STD_Ct  
    10.6847      -0.2357  

# we plug those numbers into the linear equation,
# taking the mean of the two replicates for a best estimate.

&gt; rowMeans(ct[,2:3]) * -.2357 + 10.6847
[1] 5.130429 5.210567 5.210567 4.595390 5.469837 6.582341
[7] 6.636553 6.391424


So those are log10(copy number) estimates, of a sort. You can get much more elaborate, of course.