Low Fraction of usable antibody reads in CiteSeq
We have also seen a very similar issue and are also investigating why. Possibly of note, I tried forcing the cellranger program to assign a very large number of cells (10x the actual number of cells we had) using the --force cells option. This appeared to increase the % of usable Ab reads and Ab reads per cell metrics, suggesting that these reads may be associated with barcodes that have relatively few UMIs?