Practical use of RNA structure
Please look up flavivirus 'double loops' as you described them previously (post for "Coronavirus RNA') and associated RNA secondary structure anomalies for dengue virus and associated vaccine (Butantan) and the yellow fever virus and its vaccine (17D). If you are aware of "double loops", we must be aware of the association of RNA secondardy structure and attenuated strains, i.e. live vaccines. The point is that modification of the RNA secondary structure is a key strategy in flavivirus attenuation.