Does this microstepping test result indicate need for TL smoothers?
It's worth remembering that the smaller the fraction of the step, the less torque is going to be available at that step. here is one link but there are many others that explain it as well. it looks to me like you have inconsistent friction in your system, or possibly some flex/warping of the frame. it might also be easier to see with a smaller nozzle

edit because i forgot to actually answer the question: no, by itself it does not indicate that smoothers would help. this does not mean that they wont, just that this does not look definitive to me