ILI9341 backlight flickers when using USB power from Laptop
That happens because not all USB ports can supply more than 500mA of current.
When you use a charger's USB port, it is designed to provide more than 500mA of current(ex. 1-2amps in most chargers). Try using a USB port that can provide more than 500mA of current. Most PCs and Laptops will only supply 500mA at maximum unless specified so. 

More information regarding USB power can be read here on Wikipedia.

Cheers!