N Queens in Python using backtracking
PEP 8

There is a generally accepted style for Python known as PEP 8. Most notably camelCase functions/methods should become snake_case. There are some other issues with stylistic conventions, I would give the document I linked to an overview.

Main Function

I would advise you add a main function to your program. The main reason being: If I want to use the functions from your script, when I import your script I probably don't want to actually run your program, just use generateSolution and isSafe. So:

N = int(input())
startCol = 0
board = [[0 for i in range(N)] for j in range(N)]
# print(board)

if generateSolution(board, startCol) == False:
    print("No Solution Exists")
else:
    print("Solution exists")
    print(board)


Becomes:

def main():
    N = int(input())
    startCol = 0
    board = [[0 for i in range(N)] for j in range(N)]

    if generateSolution(board, startCol) == False:
        print("No Solution Exists")
    else:
        print("Solution exists")
        print(board)

if __name__ == '__main__':
    main()


Avoid global

You should almost always not use the global keyword. In this case: just pass N into your generateSolution function, i.e. generate_solution(board, col, N).

Naming

I am not entirely sure how your code works (I am, ashamedly, unfamiliar with the N-Queen problem). I assume N refers to the number of queens? I would recommend that you call it queen_count or something of the sort. In addition, generate_solution is rather unspecific. I would call it (possibly) n_queen_solvable.

Does generate_solution actually do that?

I mentioned changing it to the name n_queen_solvable because you have return a truth value. I would expect a function like that to actually give me a configuration, not answer whether a solution exists or not. You may want to refactor this into two functions: n_queen_solvable and gen_queen_config or something of the sort.

Default parameters

You seem to want to start at the zeroith column. Makes sense. Instead of explicitly passing the startCol explicitly, I would just make col default to 0. This is done by changing:

def generateSolution(board, col):


to 

def generateSolution(board, col=0):