Autoclicker Tkinter Program
You are using global variables and connecting them with the functions StateFalse and StateTrue. It would be better to read and write the configuration options to a file.

def set_option(value):
    with open(CONFIG_FILE,"w+") as f:
        f.write(value)

def get_option():
    with open(CONFIG_FILE) as f:
        return f.read() == "True"


Where CONFIG_FILE is a filename of your choice that you choose to store the "state" (maybe you should find a better name).