Jumble solver algorithm
Hum, first of all, there are way too many comments. People simply won't read them, you should only keep the meaningful ones. For example, you could remove:

if sorted_word in dict: #Check if sorted_word is already a key


Anybody who knows how Python dictionaries work will know what this lign is doing, the code is explicit by itself. Instead of always repeating what your dict is doing, you should just explain once what it does when you declare it; the code will tell the rest. You should only explain once at the beginning how your algorithm work and let your code tell the rest. Honestly, you could remove almost all of your comments. Python is designed to be readable and it is.

Second point, avoid to name a variable dict: it is already the name of a built-in type. It's bad pratice and can be confusing for people who will try to read your code.

Also, you open your file once, but never close it. You should close it once you have filled your dict. You could use the with open('...', 'r') as f: construct to have your file closed automatically at the end of the with block, therefore, you won't even have to remember to close it. Also, file, like dict is also the name of a built-in type in Python, find another name :)