Detection limit for log-log calibration curves
I can add some points for the OP which are too long to serve as comments. I have talked to a couple of leading persons who work with a lot a of LODs. The first lesson, which is important, that there is no &quot;the limit of a detection&quot; of an analytical procedure. Second, some analytical chemists also state that it should be abolished because of the same reason- there is no the limit of detection. Limit of detection varies from method to method of calculation. You will find a couple of common things in legally accepted methods for LODs as the German DIN-32645, and ISO-BS 11843-2:2000 (Incorporating corrigendum October 2007). They all deal with linear calibration curves and no one want to mess around with quadratic or log transformed calibration curves. In the literature, in my opinion, LOD is the most incorrectly reported number and the experts agree with this opinion in personal conversations.
Now quadratic calibration curves are completely kosher. They are being used in atomic absorption spectrophotometers for ages (~ 40 years). Similarly, another HPLC detector based on light scattering does a small manipulation that makes the calibration curve non-linear. These detectors are used in pharmaceutical industry all the time. So, if your sole purpose is to do quantification, within 1 to 500 mg/L, this calibration curve should serve you well but do not calculate the LODs using the formula 3.3xsigma/slope. The problem is the determination of sigma, and the multiplying factor. When Kaiser developed these formulae (mostly in German papers), he never bothered to say anything about nonlinearity because nobody wanted to work with non-linear calibration curves before the computers.
The log transformation as suggested in the comments changes the noise behavior. If you really wish to determine a number called LOD for this instrument you can do the following (for the satisfaction of a novice reviewer of your paper or another higher up who wants a LOD number from you).
P.S. Do not round off concentrations and areas, the instrument readings look simulated to me because they are only changing in units of 50 and 100. Round off is a bad idea, it should only be done at the end.
a) Check the linearity range of your method. I plotted the data up to 50 mg/L. It is linear.
b) Obtain the readings in the linear range in triplicate or quadruplicate. You already have this data.
c) Look at the standard deviation of peak area vs. concentration curve. As you can see it is not a flat line (slope of 0). Hence the Kaiser's formula fails right here. You cannot apply 3.3*sigma/slope formula. The noise is heteroscedastic. Kaiser's formula is valid for homoscedastic noise.
Suggestions to obtain an LOD of your method.
d) Make about 7-8 standards in the range 0.1 mg/L to 20 mg/L. Repeat the process a to c. What we need is a standard near the estimated LOD.
e) If you still see a non-zero slope for the peak area standard deviation vs. concentration, apply a weighted least squares method. ISO-BS 11843-2:2000 has all the solved examples.