How to quantify amount of pigments in plant leaves?
Once you've found a suitable mobile phase in analytical TLC, you could upscale the separation and transfer the results to preparative TLC or column chromatography.

In the case of column chromatography, collect the eluted material in test tubes, check the content by analytical TLC and/or UV-VIS spectroscopy, unite identical fractions, evaporate the solvent and determine the weight of the respective fractions.

In the case of preparative TLC, scratch the separated bands off the plate, elute with a solvent of your choice and proceed as above.

If necessary, repeat the chromatographic separations for the different compound classes with other solvent mixtures.