Resolution of enantiomers? How to determine chiral compounds apart from each other?
If the compounds have never been made before, then the enantiomers have to be separated prior to analysis. Then analytical and spectroscopic techniques must be used. The R and S configurations cannot even be assigned until the relative stereochemistry had been elucidated. This is usually done by specific NMR experiments. Chiral HPLC can be used to give enantiomeric purity.