The group analyzed the genomes of 12 pure gray wolves from areas with no coyotes, three coyotes from areas where there were no gray wolves, as well as six eastern wolves and three red wolves. They were looking for any mysterious genetic material that was not gray wolf or coyote, and could be uniquely considered red wolf or eastern wolf. ||||| Gray wolves are currently protected under the U.S. Endangered Species Act (and are not always gray). 
 
 Research by UCLA biologists published today presents strong evidence that the scientific reason advanced by the U.S. Fish and Wildlife Service to remove the gray wolf from protection under the Endangered Species Act is incorrect. 
 
 A key justification for protection of the gray wolf under the act was that its geographic range included the Great Lakes region and 29 Eastern states, as well as much of North America. The Fish and Wildlife Service published a document in 2014 which asserted that a newly recognized species called the eastern wolf occupied the Great Lakes region and eastern states, not the gray wolf. Therefore, the original listing under the act was invalid, and the service recommended that the species (except for the Mexican gray wolf, which is the most endangered gray wolf in North America) should be removed from protection under the act. 
 
 A decision by the U.S. Fish and Wildlife Service to remove the gray wolf from protection under the Endangered Species Act may be made as early as this fall. 
 
 In the new study published in the journal Science Advances, biologists analyzed the complete genomes of North American wolves — including the gray wolf, eastern wolf and red wolf — and coyotes. The researchers found that both the red wolf and eastern wolf are not distinct species, but instead are mixes of gray wolf and coyote. 
 
 Reed Hutchinson/UCLA Bridgett vonHoldt and Robert Wayne in 2009. 
 
 “The recently defined eastern wolf is just a gray wolf and coyote mix, with about 75 percent of its genome assigned to the gray wolf,” said senior author Robert Wayne, a UCLA professor of ecology and evolutionary biology. “We found no evidence for an eastern wolf that has a separate evolutionary legacy. The gray wolf should keep its endangered species status and be preserved because the reason for removing it is incorrect. The gray wolf did live in the Great Lakes area and in the 29 eastern states.” 
 
 Once common throughout North America and among the world’s most widespread mammals, the gray wolf is now extinct in much of the United States, Mexico and Western Europe, and lives mostly in wilderness and remote areas. Gray wolves still live in the Great lakes area, but not in the eastern states. 
 
 Apparently, the two species first mixed hundreds of years ago in the American South, resulting in a population that has become more coyote-like as gray wolves were slaughtered, Wayne said. The same process occurred more recently in the Great Lakes area, as wolves became rare and coyotes entered the region in the 1920s. 
 
 The researchers analyzed the genomes of 12 pure gray wolves (from areas where there are no coyotes), three coyotes (from areas where there are no gray wolves), six eastern wolves (which the researchers call Great Lakes wolves) and three red wolves. 
 
 There has been a substantial controversy over whether red wolves and eastern wolves are genetically distinct species. In their study, the researchers did not find a unique ancestry in either that could not be explained by inter-breeding between gray wolves and coyotes. 
 
 “If you did this same experiment with humans — human genomes from Eurasia — you would find that one to four percent of the human genome has what looks like strange genomic elements from another species: Neanderthals,” Wayne said. “In red wolves and eastern wolves, we thought it might be at least 10 to 20 percent of the genome that could not be explained by ancestry from gray wolves and coyotes. However, we found just three to four percent, on average — similar to that found in individuals from the same species when compared to our small reference set.” 
 
 Dave Mech Red wolf 
 
 Pure eastern wolves were thought to reside in Ontario’s Algonquin Provincial Park. The researchers studied two samples from Algonquin Provincial Park and found they were about 50 percent gray wolf, 50 percent coyote. 
 
 Biologists mistakenly classified the offspring of gray wolves and coyotes as red wolves or eastern wolves, but the new genomic data suggest they are hybrids. “These gray wolf-coyote hybrids look distinct and were mistaken as a distinct species,” Wayne said. 
 
 Eventually, after the extinction of gray wolves in the American south, the red wolves could mate only with one another and coyotes, and became increasingly coyote-like. 
 
 Red wolves turn out to be about 25 percent gray wolf and 75 percent coyote, while the eastern wolf’s ancestry is approximately 75 percent gray wolf and 25 percent coyote, Wayne said. (Wayne’s research team published findings in the journal Nature in 1991 suggesting red wolves were a mixture of gray wolves and coyotes.) 
 
 Although the red wolf, listed as an endangered species in 1973, is not a distinct species, Wayne believes it is worth conserving; it is the only repository of the gray wolf genes that existed in the American South, he said. 
 
 The researchers analyzed SNPs (single nucleotide polymorphisms) — tiny variations in a genetic sequence, and used sophisticated statistical approaches. In the more than two dozen genomes, they found 5.4 million differences in SNPs, a very large number. 
 
 Carla Schaffer/AAAS 
 
 Genomic sequencing reveals that red wolves and eastern wolves are hybrids of gray wolves. 
 
 Wayne said the Endangered Species Act has been extremely effective. He adds, however, that when it was formulated in the 1970s, biologists thought species tended not to inter-breed with other species, and that if there were hybrids, they were not as fit. The scientific view has changed substantially since then. Inter-breeding in the wild is common and may even be beneficial, he said. The researchers believe the Endangered Species Act should be applied with more flexibility to allow protection of hybrids in some cases (it currently does not), and scientists have made several suggestions about how this might be done without a change in the law, Wayne said. 
 
 Co-authors of the study include lead author Bridgett vonHoldt, an assistant professor at Princeton University and former UCLA graduate student and postdoctoral scholar who worked in Wayne’s laboratory; Beth Shapiro, UC Santa Cruz associate professor of ecology and evolutionary biology; Jacqueline Robinson, a UCLA graduate student in ecology and evolutionary biology in Wayne’s laboratory; and Zhenxin Fan, an assistant professor at China’s Sichuan University, who was a visiting graduate student in Wayne’s laboratory. 
 
 The research was funded by the National Science Foundation, the Turner Endangered Species Fund, the Wilburforce Foundation, and the Morris Animal Foundation. ||||| Mark Conlin/Oxford Scientific/Getty 
 
 The red wolf, a critically endangered species living in the south-eastern US, may be nothing more than a hybrid between coyotes and the grey wolf, a new study suggests. 
 
 If so, it may lose its conservation status and protection, given that US legislation does not protect hybrids. 
 
 This could lead to loss of an important evolutionary lineage, because the red wolf is the only living repository of genes from the grey wolves that were driven near extinction in the south-eastern states by trapping and agricultural development. 
 
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 Hard to count 
 
 Zoologists have struggled for many years to decide how many species of wolves live in North America. Everyone agrees on the coyote and the grey wolf, which is found all round the northern hemisphere. 
 
 The US government also recognises two others, the eastern wolf, now found in Ontario, and the red wolf, which has been bred in captivity from 12 individuals and reintroduced to the eastern US. 
 
 The issue has been difficult to resolve, partly because bounty hunting drove eastern and southern wolf populations close to extinction a century ago, which opened their habitat for coyotes to invade and hybridise with the few wolves. As a result, these wolves carry many coyote genes, making it hard to spot any genetic markers of a separate wolf species. 
 
 Now Robert Wayne, a geneticist at the University of California at Los Angeles, and his colleagues have compared whole genome sequences of 28 wolf and coyote individuals from Eurasia and North America, including eastern and red wolves. 
 
 Mix it up 
 
 The eastern and red wolves showed no evidence of independent ancestry; their genomes could be explained solely by admixture between coyotes and grey wolves. 
 
 Previous, less comprehensive studies have found genetic and morphological reasons to recognise these wolves as separate species, and others have shown that they seem to be hybrids. Wayne’s study is unlikely to settle the matter. However, it is the highest-resolution look at the problem so far, says Roland Kays of the North Carolina Museum of Natural Sciences in Raleigh. 
 
 If Wayne’s team is correct, it could spell trouble for the red wolf. The US Endangered Species Act makes no mention of hybrids, so the red wolf might lose its protected status – and the millions of dollars spent on captive breeding and recovery programmes. 
 
 That would be unfortunate, says Wayne, because the hybrid red wolf’s small size may mean it is better adapted than a pure grey wolf to life in the south-eastern US. 
 
 “Maybe we can’t put a grey wolf in the American south-east,” says Wayne. “Maybe the top predator has to be a smaller wolf.” 
 
 The red wolf’s situation shows that the Endangered Species Act needs to catch up to reality, says Kays. “What we’re finding with today’s high-resolution genetic tools is that hybrids are everywhere,” he says. “To say it’s a hybrid, so it’s not worth protecting, just doesn’t work anymore.” 
 
 Journal reference: Science Advances, DOI: 10.1126/sciadv.1501714 
 
 Read more: American icons aren’t the animals they used to be
– The US government recognizes three wolf species in North America: the red wolf, eastern wolf, and gray wolf. Two, it turns out, aren't true wolf at all. In a new genetic study, scientists at UCLA say the gray wolf is the last wolf species on the continent and all others are simply hybrids. After studying the genomes of eastern wolves, red wolves, gray wolves, and coyotes, they found "no evidence for distinct eastern or red wolf species," study author Robert Wayne tells the Los Angeles Times. In fact, the red wolf found in the southern US has about 25% gray wolf and 75% coyote ancestry, while the eastern wolf of central Ontario has about 75% gray wolf and 25% coyote ancestry. Scientists say the two species began mixing hundreds of years ago in the American South and about a century ago in the Great Lakes area. Previous research has found genetic and morphological evidence that the red and eastern wolves are unique, so this study might not have the final word, reports the New Scientist. But it could have consequences: The gray wolf is protected under the Endangered Species Act based on a traditional geographic range including the Great Lakes region and 29 Eastern states. But in 2014, the US Fish and Wildlife Service proposed removing the animal's protections because it found the eastern wolf occupied that territory instead, making the gray wolf listing invalid. This study shows the gray wolf did live in the range and "should keep its endangered species status," Wayne says in a release. A decision could come this fall. The Endangered Species Act doesn't currently recognize hybrids, though Wayne says that should change. (The "golden wolf" was just discovered.)