Denny inherited one set of chromosomes from her Neanderthal ancestors, depicted in this model.Credit: Christopher Rynn/University of Dundee 
 
 A female who died around 90,000 years ago was half Neanderthal and half Denisovan, according to genome analysis of a bone discovered in a Siberian cave. This is the first time scientists have identified an ancient individual whose parents belonged to distinct human groups. The findings were published on 22 August in Nature1. 
 
 “To find a first-generation person of mixed ancestry from these groups is absolutely extraordinary,” says population geneticist Pontus Skoglund at the Francis Crick Institute in London. “It’s really great science coupled with a little bit of luck.” 
 
 The team, led by palaeogeneticists Viviane Slon and Svante Pääbo of the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, conducted the genome analysis on a single bone fragment recovered from Denisova Cave in the Altai Mountains of Russia. This cave lends its name to the ‘Denisovans’, a group of extinct humans first identified on the basis of DNA sequences from the tip of a finger bone discovered2 there in 2008. The Altai region, and the cave specifically, were also home to Neanderthals. 
 
 Divided by DNA: The uneasy relationship between archaeology and ancient genomics 
 
 Given the patterns of genetic variation in ancient and modern humans, scientists already knew that Denisovans and Neanderthals must have bred with each other — and with Homo sapiens (See 'Tangled Tree')﻿. But no one had previously found the first-generation offspring from such pairings, and Pääbo says that he questioned the data when his colleagues first shared them. “I thought they must have screwed up something.” Before the discovery of the Neanderthal–Denisovan individual, whom the team has affectionately named Denny, the best evidence for so close an association was found in the DNA of a Homo sapiens specimen who had a Neanderthal ancestor within the previous 4–6 generations3. 
 
 Ancestry revealed 
 
 Pääbo’s team first uncovered Denny’s remains several years ago, by looking through a collection of more than 2,000 unidentified bone fragments for signs of human proteins. In a 2016 paper4, they used radiocarbon dating to determine that the bone belonged to a hominin who lived more than 50,000 years ago (the upper limit of the dating technique; subsequent genetic analysis has put the specimen at around 90,000 years old, according to Pääbo). They then sequenced the specimen’s mitochondrial DNA — the DNA found inside cells’ energy converters — and compared that data to sequences from other ancient humans. This analysis showed that the specimen’s mitochondrial DNA came from a Neanderthal. 
 
 But this was only half of the picture. Mitochondrial DNA is inherited from the mother and represents just a single line of inheritance, leaving the identity of the father and the individual’s broader ancestry unknown. 
 
 A bone fragment was sequenced for its genome.Credit: Thomas Higham/University of Oxford 
 
 In the latest study, the team sought to get a clearer understanding of the specimen’s ancestry by sequencing its genome and comparing the variation in its DNA to that of three other hominins — a Neanderthal and a Denisovan, both found in Denisova Cave, and a modern-day human from Africa. Around 40% of DNA fragments from the specimen matched Neanderthal DNA — but another 40% matched the Denisovan. By sequencing the sex chromosomes, the researchers also determined that the fragment came from a female, and the thickness of the bone suggested she was at least 13 years old. 
 
 With equal amounts of Denisovan and Neanderthal DNA, the specimen seemed to have one parent from each hominin group. But there was another possibility: Denny's parents could have belonged to a population of Denisovan–Neanderthal hybrids. 
 
 A fascinating genome 
 
 To work out which of these options was more likely, the researchers examined sites in the genome where Neanderthal and Denisovan genetics differ. At each of these locations, they compared fragments of Denny's DNA to the genomes of the two ancient hominins. In more than 40% of cases, one of the DNA fragments matched the Neanderthal genome, whereas the other matched that of a Denisovan, suggesting that she had acquired one set of chromosomes from a Neanderthal and the other from a Denisovan. That made it clear that Denny was the direct offspring of two distinct humans, says Pääbo. “We’d almost caught these people in the act.” 
 
 Evidence mounts for interbreeding bonanza in ancient human species 
 
 The results convincingly demonstrate that the specimen is indeed a first-generation hybrid, says Kelley Harris, a population geneticist at the University of Washington in Seattle who has studied hybridization between early humans and Neanderthals. Skoglund agrees: “It’s a really clear-cut case,” he says. “I think it’s going to go into the textbooks right away.” 
 
 Harris says that sexual encounters between Neanderthals and Denisovans might have been quite common. “The number of pure Denisovan bones that have been found I can count on one hand,” she says — so the fact that a hybrid has already been discovered suggests that such offspring could have been widespread. This raises another interesting question: if Neanderthals and Denisovans mated frequently, why did the two hominin populations remain genetically distinct for several hundred-thousand years? Harris suggests that Neanderthal–Denisovan offspring could have been infertile or otherwise biologically unfit, preventing the two species from merging. 
 
 Neanderthal-Denisovan pairings could also have had some advantages, even if there were other costs, says Chris Stringer, a palaeoanthropologist at the Natural History Museum in London. Neanderthals and Denisovans were less genetically diverse than modern humans, and so interbreeding might have provided a way of “topping up” their genomes with a bit of extra genetic variation, he says. The study also raises questions over how matings between different human groups happened, says Stringer — for example, whether or not they were consensual. A more detailed account of the gene flow between Neanderthals and Denisovans in the future might offer hints into ancient human behaviour. 
 
 Missed connections 
 
 Pääbo agrees that Neanderthals and Denisovans would have readily bred with each other when they met - but he thinks that those encounters were rare. Most Neanderthal remains have been found across western Eurasia, whereas Denisovans have so far been discovered only in their eponymous Siberian cave. Although the two groups’ home turf overlapped in the Altai Mountains and possibly elsewhere, these areas would have been sparsely populated. “I think any Neanderthal that lived west of the Urals would never ever meet a Denisovan in their life,” Pääbo says, referring to the mountain range that slices through western Russia and Kazakhstan. 
 
 Denny's remains were discovered in Denisova Cave in southern Siberia.Credit: Bence Viola/Max Planck Institute for Evolutionary Anthropology 
 
 But sometimes, Neanderthal populations might have travelled from western Eurasia to Siberia, or vice-versa. On the basis of the variation in the specimen’s genome, the team deduced that Denny’s Neanderthal mother was more closely related to a Neanderthal specimen found thousands of kilometres away, in Croatia, than to another found less than 1 metre away in the same cave. 
 
 Neanderthals built cave structures — and no one knows why 
 
 The Croatian Neanderthal died ﻿died much more recently than Denny — about 55,000 years ago — while the Neanderthal from Denisova Cave is around 120,000 years old. That leaves two possibilities to explain the ancestry of Denny's mother: either a population of European Neanderthals came east to the Altai Mountains and partly replaced the region’s Neanderthals before the hybrid was born, or a group of Neanderthals could have left the Altai Mountains for Europe sometime after Denny’s birth. Either way, says Harris, Neanderthals “didn’t just stay in one place for thousands of years”. 
 
 With a Neanderthal mother and a Denisovan father, what should we call the new specimen? “We shy away a little from the word ‘hybrid’,” says Pääbo. The term implies that the two groups are discrete species of human, whereas in reality the boundaries between them are blurry — as the new study shows. Defining a species in the natural world is not always clear-cut, says Harris, and it’s interesting to see long-running debates about how to categorize organisms start to be applied to humans. 
 
 Whatever scientists decide to call Denny, Skoglund says he would have loved to be able to meet her. “It’s probably the most fascinating person who’s ever had their genome sequenced.” ||||| This undated photo provided by Bence Viola of the University of Toronto in August 2018 shows the valley above a cave where Denisovan fossils were found in the Altai Krai area of Russia. On Wednesday,... (Associated Press) 
 
 This undated photo provided by Bence Viola of the University of Toronto in August 2018 shows the valley above a cave where Denisovan fossils were found in the Altai Krai area of Russia. On Wednesday, Aug. 22, 2018, scientists reported in the journal Nature that they have found the remains of an ancient... (Associated Press) 
 
 BERLIN (AP) — Scientists say they've found the remains of a prehistoric female whose mother was a Neanderthal and whose father belonged to another extinct group of human relatives known as Denisovans. 
 
 The 90,000-year-old bone fragment found in southern Siberia marks the first time a direct offspring of these two groups has been discovered, according to a study published Wednesday in the journal Nature. 
 
 Both groups disappeared by about 40,000 years ago. Neanderthals lived in Europe and Asia, while fossils of Denisovans are known only from the cave where the fragment was found. 
 
 Past genetic studies have shown interbreeding between the two groups, as well as with our own species, which left a trace in the DNA of today's people. But the new study is the first to identify a first-generation child with Neanderthal and Denisovan parents. 
 
 "It's fascinating to find direct evidence of this mixing going on," said Svante Paabo, one of the study's lead authors. 
 
 Paabo said he was surprised by the discovery, given how relatively few remains of our evolutionary relatives have been found around the world. 
 
 The cave near Mongolia where the bone was found contains some remains attributed to Neanderthals as well as Denisovans. But finding an actual offspring of the two groups — which are more different from each other than any two present-day human groups — seemed like a rare stroke of luck, Paabo said. 
 
 "The fact that we stumbled across this makes you wonder if the mixing wasn't quite frequent," said Paabo, a geneticist at Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany. 
 
 The finding doesn't reveal how often such mating occurred and where, said Ron Pinhasi, a physical anthropologist at the University of Vienna who wasn't involved in the study. 
 
 "Had it happened frequently, we would not have such divergence between the Denisovans and Neanderthal genomes," he said. 
 
 The newly discovered DNA could be interpreted in different ways, said Anders Eriksson, evolutionary population geneticist at King's College London who wasn't involved in the study. 
 
 "I think they convincingly showed that genetically this individual falls halfway between the Neanderthal and Denisovan fossils found in the same cave," he said. "But I'm less convinced that it is necessarily a first-generation offspring of a union between Neanderthal and Denisovan." 
 
 The fossil could instead have come from a population with roughly an equal mix of Neanderthal and Denisovan ancestry, he said. It will take analysis of more fossils to find out, he said. 
 
 The researchers said the small bone fragment likely came from the arm or leg of a female who was at least 13 years old at the time of death. Comparison with other ancient DNA showed that the genes she inherited from her mother were more closely related to Neanderthals who lived later in Europe than to other Neanderthal remains found in the cave, suggesting a wave of westward migration. 
 
 Together with previously discovered remains of a Homo sapiens that had a Neanderthal ancestor four to six generations earlier, Paabo said the latest find supports the theory that the now-extinct ancient lineages may have been absorbed through interbreeding with modern humans, rather than wiped out through warfare as is widely believed. 
 
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– Scientists say they've found the remains of a prehistoric female whose mother was a Neanderthal and whose father belonged to another extinct group of human relatives known as Denisovans, the AP reports. The 90,000-year-old bone fragment found in southern Siberia marks the first time a direct offspring of these two groups has been discovered, according to a study published Wednesday in the journal Nature. Both groups disappeared by about 40,000 years ago. Neanderthals lived in Europe and Asia, while fossils of Denisovans are known only from the cave where the fragment was found. Past genetic studies have shown interbreeding between the two groups, as well as with our own species, which left a trace in the DNA of today's people. But the new study is the first to identify a first-generation child with Neanderthal and Denisovan parents. "It's fascinating to find direct evidence of this mixing going on," says Svante Paabo, one of the study's lead authors. Paabo says he was surprised by the discovery, given how relatively few remains of our evolutionary relatives have been found around the world. The cave near Mongolia where the bone was found contains some remains attributed to Neanderthals as well as Denisovans. But finding an actual offspring of the two groups—which are more different from each other than any two present-day human groups—seemed like a rare stroke of luck. "The fact that we stumbled across this makes you wonder if the mixing wasn't quite frequent," says Paabo. Click for the full story.