Margherita 's siblings included Ferdinando II de ' Medici , Grand Duke of Tuscany , Cardinal Gian Carlo de ' Medici , Cardinal Leopoldo de ' Medici and Anna , wife of Ferdinand Charles , Archduke of Further Austria . Margherita 's siblings included : Ferdinando II de ' Medici , Grand Duke of Tuscany , Cardinal Gian Carlo de ' Medici , Cardinal Leopoldo de ' Medici and Anna , wife of Ferdinand Charles , Archduke of Further Austria .