Below is an context that describes a sql query, paired with an question that provides further information. Write an answer that appropriately completes the request.  # noqa: E501
### Context:
CREATE TABLE table_name_16 (against INTEGER, opposing_teams VARCHAR, venue VARCHAR, status VARCHAR)
### Question:
What is the lowest against score for Twickenham, London with the status of Five Nations against Ireland?
### Answer:
SELECT MIN(against) FROM table_name_16 WHERE venue = "twickenham, london" AND status = "five nations" AND opposing_teams = "ireland"