PROJECT TITLE ANALYTICAL InvESTIGATIUNS PERIUD coveRED APRIL MaY 15 , 1981 WRITTeN Lecoultre-E (ETL) ORGANIC ACIDS BY_GC chromatogfaphic Work Ehe developnent thod for Ehe 4as analysig citric lactic nalic succini fumaric oud artaric oxalic fermented tobacco Pyruvic and acids requested xtracts Biotechno (1) , been ogy has comp leted_ chromatogram typica gas experimental samp ]e shown figure the presently being PME Analytical Method prepared ORGANIC ACIDS AND PHENOLS organic 1on York the determinat_ extension Ma tobacco fermented analytical procedure extracts range the 9] muitaneous analy818 Yide aliphatic C18 and romatic mono carboxylic acids and of) phenols 03 8cc and tobacco smoke condensale presently estigated The thod involves team distillation Iny step (clean-up der vat izat and acids pheno] $ extractive kylation penta-fluorobenzy lbromine using tetrabuty lammoniur pentafluorobenzy_ ion and analysis esters by (GC) and' pent a luorobenzy 1 ethers and detect 0n SERVICE Q[HERGRQUPS (GC)2 ( 2) ( 3) . 51 X acetin samples ysedi for Qc by Knn 4014 Phosphate su lfate 8 5 samples 1 Tor and determined Kere echno B0 ogy ysed 1"5 Amino Pra_ SAvOURY H? FFTe sumo 0Ct 12
PROJECT TITLE ANALYTICAL InvESTIGATIUNS PERIUD coveRED APRIL MaY 15 , 1981 WRITTeN Lecoultre-E (ETL) ORGANIC ACIDS BY_GC Work Ehe developnent 4as chromatogfaphic thod for Ehe oud analysig citric lactic nalic succini fumaric artaric Pyruvic and oxalic acids fermented tobacco xtracts requested Biotechno ogy (1) , has been comp leted_ typica gas chromatogram experimental samp ]e shown the figure presently being prepared PME Analytical Method ORGANIC ACIDS AND PHENOLS extension Ma York the determinat_ 1on organic fermented tobacco extracts analytical procedure the 9] muitaneous analy818 Yide range aliphatic C18 and romatic mono carboxylic acids and 03 of) phenols 8cc and tobacco smoke condensale presently Iny estigated The thod involves team distillation step (clean-up der vat izat acids and pheno] $ extractive kylation using penta-fluorobenzy lbromine tetrabuty lammoniur ion and analysis pentafluorobenzy_ esters and' pent a luorobenzy 1 ethers by (GC) and detect 0n SERVICE Q[HERGRQUPS acetin samples Knn 4014 ysedi for Qc by (GC)2 ( 2) ( 3) . 51 X Phosphate and su lfate Kere determined B0  echno ogy 8 5 samples Tor sumo 1"5 Pra_ 0Ct SAvOURY 1 FFTe H? ysed Amino 12