Furhuccd Aanceltunil Rtrrami endo Drrarimen chm For Oliciul Smok8u6} Volatile Acids of Cigar 39022 Irwln Schmeltz' and william Schlothauer' Eastern Regional Research Laboratory; ? Philadelphia /8, Pennsyivania; U.SA: 1959 , bilicic ncid In 2 ml (Scheparlz; mplc described entlier latoduclion 1960) . The emokc glrcine wolulon collected qinUtalvely AMholeo cizretle smokc canled acelone-dn chlorolom Kran ; Lrip? investigaled eIlrrively Ior the DTrs- the cojumn; and (he columd cifar #ert ernel Uvo Foculr orndb rolatile acids TaVc WFha panalell #lth all-Havana ruccexsitely eluled *in emokcd: eimilr sudiea deecribing npnrirrd 10 and 25 [er Irouty Iller _0 dmztn LlcX; 4id nemecin The lower eliphallc cirar BMokL Finall chlorolarm- cut fllct bicndr come lic cana ALids cirarch Danuke additlcral 100 the 26 per cent Lotacro9 Ujuwlly cifar? Eaerl inolated deniinnn lhrourh the aelulion #u: Lhcn Pueed vir-Idcd nultiri-nl Rinbke conucnaule Worxets Rcccnil; 121 n Froun: Aai ulumn Trn millr Iraclons Wen (1954 Kobishi eohuy urated ornenctin Loxat6 Ncle 4ii> ircm Fau Hnti The Fmickt "Lhnliuic Ahiurn nvdrozidc uginx [rups conainio addilion mrthtltaleric bumnthymd hlue_ Wuskeed Fcrrteircl De tdicator cenAle' Hute Knrir identifctian naaee the elution dala indicated tnolul nehalol ethr; cttsnilnc mndi Wt"iTlIOI sodium hydrxide aulu- ruo Minnr ntlks (Ficure Wnjur #lU JnolhereudrPuzke derivatirc * The elutlon the two The   elhcr  exlract #08 Tatn FUNe fionz; remnried and hic co-Tork"ts cirars wer bimilor each #ith circcn aikoli Tcnotc bmnd: the Fresence in cigarelle Fn ncidic matcrial; Thc methinolic eolu- other Galnie thtIAls  acid< irom al. (1957) for the volallle alalint unake mnda sddition ocid Linzolc using clkrtle emoke. Acording hvdmride golulon IeIda enonm chromalozraplie Gnmn The thost #orkerk, Lhe frat peak con- 3oltuti etmnan Wncio; idcntincilion Tac atter Tneenede hicher of  Propi- homolozuea Te meencmu Tatt Han on ntludco numnansc acid: thc decond Oeacideand othcr ertrcicd rLhrr Iehnou underro 4 Anfe cifar Hnunaa All the mhlnd Fllaln trala and Dlscs anim- Iik ppionc diderni fmni mcinc d nrocca ina lour were (hen pooled neuk Hons ICetc riertrinc Anharcn ARGC alkaline aolulion Thc combined fnnir iormintior rnilanle Acids: Thr pnks conccntraled Folume Idcutnallon Vollill comnosition Gifir pH 20 #il pkos- Imlncidifen Mtn chrunntognphed rant umakr Nuu cuch cullatijucmis distilled, One inf trer dnertnt s/vent stulcm:- Mhoric Fcam ~eppenred Wulenai halt lilers dialln Unamaliconcen Fited BinM nresenis quilitalire tezeni TETTI (IQH) (Kennedy aud Fcrc coliccico lr POLenITO Wmmionm Ihr nuijor qunntiatiro 1951) nictricat autter Folallc neida Jmoko Cear alcanolunterin ctiub hedroxide chimalc Au ccnsil (Bur Holh Ruem-Volatile aciditr nter nmntuint (uu:5i ol , 1957} Hrclton Teauitine neutrai Eporimontal Hlnol " izr*'| distillale wilh eher,' thc Jiatil ~butyl ckohol / Colirction Cigare 'ere Iate"e dnerx Velc cucFurald ammonin Oa: Smak: eunmore brann Teridue andium 9ly (SciWrUmxn_ 196u } cauumnnicd cn *CC; SlccA-id nccionciyal conc#nirat rclative humidiv and CntunaturEW (96:6;1 - (Kcneed Rur Thy amoking amokcd chremtogmphic ntarcqun alnmonm Utm (19671 #08 opolicd Barker, 1961} _ Spray the   auicmatic emtokint Dcina prtrinicnini conniliona mxlu Idium Falta, Houtrcr necni' contireu lTum aane bluc; 200 mg cltric Ihc sllicic acid W1s unWahcd bromcnc7ol 20 =} cthanol ar; Uhavolumn Kunrer Lbsolutc preTred by mkxlnp The colunin #a5 VGrni- Keot Int Eclvcnt suitcm coniaining DetaIcCneem Etiirs+i ncid #ith 36 0.1 per ailicic nrontlimunc ninny M -krin adjusted pH 20 #ith chlorolform, 7*J Icle concrhiralnl hydruchluric acid Folloixing columo cnrc( - 5 FALTT Itijua rring (he resulting pondut ~'mratien cuer". clma Krk Finehe Iats cnlarelorm nno peuring (ne Jurri tariisnmane mohd trapralid 6t of(he columa Butr OC
Furhuccd Aanceltunil Rtrrami endo Drrarimen chm For Oliciul Volatile Acids of Cigar Smok8u6} 39022 Irwln Schmeltz' and william Schlothauer' Eastern Regional Research Laboratory; ? Philadelphia /8, Pennsyivania; U.SA: latoduclion described entlier (Scheparlz; 1959 , mplc bilicic ncid In 2 ml 1960) . The emokc collected glrcine wolulon AMholeo cizretle smokc canled acelone-dn chlorolom qinUtalvely Lrip? Kran ; investigaled eIlrrively Ior the DTrs- Uvo Foculr orndb cifar #ert ernel the cojumn; and (he columd rolatile acids WFha TaVc emokcd: panalell #lth all-Havana ruccexsitely eluled *in npnrirrd deecribing eimilr sudiea Iller _0 dmztn LlcX; 4id nemecin 10 and 25 [er Irouty cirar BMokL The lower eliphallc cut fllct bicndr come lic cana chlorolarm- Finall ALids cirarch Danuke Lotacro9 Ujuwlly cifar? additlcral 100 the 26 per cent inolated deniinnn Eaerl vir-Idcd nultiri-nl Rinbke conucnaule aelulion #u: Lhcn Pueed lhrourh the Froun: Worxets Rcccnil; 121 n Aai ulumn Trn millr Iraclons Wen Kobishi (1954 eohuy ornenctin Loxat6 Ncle urated Hnti Fau 4ii> ircm The [rups conainio Fmickt "Lhnliuic Ahiurn nvdrozidc uginx addilion mrthtltaleric cenAle' Hute Wuskeed Fcrrteircl bumnthymd hlue_ De tdicator Knrir naaee identifctian ethr; tnolul nehalol the elution dala indicated mndi Wt"iTlIOI cttsnilnc sodium hydrxide aulu- Wnjur #lU ruo Minnr ntlks (Ficure derivatirc * JnolhereudrPuzke The   elhcr  exlract #08 Tatn The elutlon FUNe the two and hic co-Tork"ts fionz; remnried circcn #ith aikoli Tcnotc bmnd: cirars wer bimilor each the Fresence in cigarelle Fn ncidic matcrial; Thc methinolic eolu- other Galnie thtIAls  acid< irom mnda alalint unake al. (1957) for the volallle sddition Linzolc ocid using enonm hvdmride golulon IeIda clkrtle emoke. Acording Gnmn chromalozraplie 3oltuti etmnan Wncio; The thost #orkerk, Lhe frat peak con- Tneenede idcntincilion Tac atter Te meencmu Tatt Han hicher homolozuea of  Propi- on ntludco numnansc ertrcicd rLhrr Iehnou Oeacideand othcr acid: thc decond Anfe cifar Hnunaa underro 4 trala and Dlscs All the Fllaln anim- Iik ppionc mhlnd mcinc d nrocca ina diderni fmni Hons were (hen pooled neuk ICetc lour riertrinc Anharcn ARGC Thc combined alkaline aolulion fnnir iormintior rnilanle conccntraled Folume Idcutnallon Acids: Thr pnks Vollill comnosition Gifir Imlncidifen pH 20 #il pkos- Mtn chrunntognphed rant umakr Nuu  cuch cullatijucmis Mhoric Fcam distilled, One inf trer dnertnt s/vent stulcm:- ~eppenred Wulenai halt lilers BinM dialln Unamaliconcen Fited tezeni TETTI nresenis quilitalire Fcrc coliccico lr POLenITO Wmmionm (IQH) (Kennedy aud qunntiatiro Ihr nuijor nictricat autter 1951) Folallc neida Cear Jmoko ctiub hedroxide chimalc Au alcanolunterin ccnsil Holh Ruem-Volatile aciditr nter nmntuint (uu:5i (Bur Hrclton Teauitine neutrai ol , 1957} Eporimontal izr*'| distillale wilh eher,' thc Jiatil ~butyl ckohol / Hlnol " Colirction Smak: Cigare 'ere Iate"e eunmore dnerx Velc cucFurald ammonin Oa: cauumnnicd cn *CC; brann Teridue andium 9ly (SciWrUmxn_ 196u } rclative humidiv and SlccA-id CntunaturEW nccionciyal conc#nirat amoking Thy Utm amokcd chremtogmphic ntarcqun Rur alnmonm (96:6;1 - (Kcneed Dcina the   auicmatic emtokint (19671 #08 opolicd Barker, 1961} _ Spray aane prtrinicnini conniliona mxlu Idium Falta, Houtrcr necni' contireu lTum Ihc sllicic acid W1s unWahcd bromcnc7ol bluc; 200 mg cltric Uhavolumn Kunrer 20 =} Lbsolutc cthanol ar; VGrni- Keot The colunin #a5 preTred by mkxlnp Int Eclvcnt suitcm coniaining DetaIcCneem Etiirs+i ailicic ncid #ith 36 nrontlimunc 0.1 per ninny Icle M -krin adjusted pH 20 #ith chlorolform, 7*J concrhiralnl hydruchluric acid FALTT Folloixing columo cnrc( - Itijua 5 rring (he resulting pondut clma Krk ~'mratien cuer". cnlarelorm nno peuring (ne Jurri tariisnmane Finehe Iats 6t columa Butr of(he mohd trapralid OC