75 DETER"ISINC FITRIC OXIDE I SHOFE A; ALTOYATED KZIHOD OF Jenkins nd Canon of detemining nitric oxide develo? 2ufozated pethod Our goa] Kas tapid Puff smoke_ The techaique rhich ve use Prorides Puff spoked These analysis of nitric oxide fIom 20 cigarettes Smoke conditions_ The accocplished by under standard Puffing analysis is modified srtinge coupling cherflusine ez: nftfic oxicc onalyzer snoking Thc chinc altered [o purge Itself vith machine b0cking Ca $ This cycle dilures the smoke vhich fresh aiv after each Purzing Puff Problens resul [ing NQ decay_ retaids the al50 effectivel} eliminafes the standard Iroz the relatively large Joterral volupe cf design. repotted in the Several mechods of itric oride 37a]5515 have heen Most literarure chronatczaphy chenflurinescence aro 8a5 15 being dont the work cheniluoines used ccne Inore Tecent British-American Tobacco Ccmpan}_ chediludinescent analyzer made by the This enalyzer 15 certertly Darketed by Filtrona (SLIDE 1) analyzer to establish its stability First_ exarined and Analyzer stabflicy i[s afcuracy evaluated using {our detercinc 525 nitric oxide (199.L 388 808 and 1196 Ppo'6) levels in nitrogen ten-day perio] These satples rere analyzed [Ficc day Duet (peak attenuation} versUs nitric analysis of responsc heigh: deielot 8by day variations cxide concenlralion shcxed that significant driftine occurted lum This $5 based cr the Concecitirion ary shorn In Sl1d0 Vaues tigzted variance The calculated under the colunn als0 anc LJ5 shhoct Fron these estirared listed variances chat the higher cee the stancerc level (X) the Teliable the Tesponse 4ecs caeuteent (X) result of this least squares Yeignted approach 52s u520 fit data thc colibration line Y Thc {tted equation 16 shoxn the Elide. next (SLIDE 2) are respectively equal The standard the esticafes and ertote 3065 and 001 reactive compound Nitric oxide The decay rlch tine of nittic 7 result teactions vith oxfde In stokc other smoke oxygen been wel1 docurenteds It has al50 been dewonstrated that nas cotoonents dilucion of swoke vith air etards this decay. This result of dilution vich che third order oxidation reaction of nittic oxide consistent ait. (SLIDE Ve used detector the decay nitric oxide In Yho] smoke our examine dfluted vfth The and sdoke egual volune a1 . systec uSCO Bhoxn cbis slIde.
75 A; ALTOYATED KZIHOD OF DETER"ISINC FITRIC OXIDE I SHOFE Jenkins nd Canon Our goa] Kas develo? 2ufozated pethod of detemining nitric oxide smoke_ The techaique rhich ve use Prorides tapid Puff Puff analysis of nitric oxide Smoke fIom 20 cigarettes These spoked under standard Puffing conditions_ The analysis is accocplished by coupling cherflusine ez: nftfic oxicc onalyzer modified srtinge snoking machine Thc b0cking chinc Ca $ altered [o purge Itself vith fresh aiv after each Puff This Purzing cycle dilures the smoke vhich retaids the NQ decay_ al50 effectivel} eliminafes Problens resul [ing Iroz the relatively large Joterral volupe cf the standard design. Several mechods of itric oride 37a]5515 have heen repotted in the literarure 8a5 chronatczaphy aro chenflurinescence Most the Inore Tecent work 15 being dont cheniluoines  ccne used chediludinescent analyzer made by the British-American Tobacco Ccmpan}_ This enalyzer 15 certertly Darketed by Filtrona (SLIDE 1) First_ exarined analyzer to establish its stability and detercinc i[s afcuracy Analyzer stabflicy 525 evaluated using {our levels nitric oxide in nitrogen (199.L 388 808 and 1196 Ppo'6) These satples rere analyzed [Ficc day Duet ten-day perio] analysis of deielot responsc (peak heigh: attenuation} versUs nitric cxide concenlralion shcxed that significant day 8by variations driftine occurted ary Concecitirion This $5 based cr the lum Vaues shorn In Sl1d0 The tigzted variance LJ5 als0 calculated anc shhoct under the colunn listed Fron these estirared variances cee chat the higher the stancerc level (X) the 4ecs Teliable the caeuteent Tesponse (X) result of this Yeignted least squares approach 52s u520 fit data thc colibration line Y Thc {tted equation 16 shoxn the next Elide. (SLIDE 2) and are respectively equal The standard ertote 3065 and 001 the esticafes Nitric oxide reactive compound The decay rlch tine of nittic oxfde In stokc result teactions vith oxygen other smoke cotoonents nas been wel1 docurenteds It has al50 been dewonstrated that dilucion of swoke vith air etards this decay. This result of dilution consistent vich che third order oxidation reaction of nittic oxide ait. 7 (SLIDE Ve used our detector examine the decay nitric oxide In Yho] smoke and sdoke dfluted vfth egual volune a1 . The systec uSCO Bhoxn cbis slIde.