4479 Characterization of the Inilammatory Reaction in the Peripheral Airways of Cigarette Smokers Using Immunocytochemistry' -, FLE Licy ctr. Gaes CAROL BOSKEN Aizn~[ JENNIFER HARDS, Kevin GATTER and James HoCAc : Introduction Igarcitc smoking causcs Summart Lundliasue tom ?0*hents Unosnjoing totaction JUI Inllamma tor #penphorlcaiinomtrs studiod tory mcacon ving monoclnoi ~Ll anllboolen and Jirwals Idonlln tnaony Geli Non produccs airwzy obstruclion detnnico Dationer Alrer Eeentlonn approxi oloncemncay iate Anclatoolmphe (BLLT mately IS t0 20g0 0f acrording Une pallenba amupad paneoniptoictodFev, (#LFFV ) Into hcav1 smokers (2). obatnuclednFFy B01d1 Gnd Contro  (#Fv , noerl Tno Dlocled Iu7ge This obs[ruciion ett locatcd Wulled Juuie cnembada the petiph- mtolulnuerTel Ilourd nitroarnn A_tror allc #0 Inlc airways Tcm lugutt] thoughi IO be c80 Lulno B4no L0t Ton 407101 hlatologk #uGIlo.na mplad ubIno com Dom Irom (heto semolou Iuno blaintd wiln Monocionas innammatory (4-6)- pIOCES EpecIIc inIlde antlboclax mloncelityperehlch Morphomctric studies ofthechronic Ji- rounicd O*pogiledpot BQu*7} Munimeto( amm tall ar Ten anonted pntienla Wun way inflammation in smokers Aniat obalncuon nsd moro @-lymphonttt nindy Inthe bcen RovenIllla thon Jldth have contrclJublacin based on classic histologic atalhenurt polmoraonuciear Jaupmuconal cxaminauon IoukooNar (nIed lo tho amouniumorid hax 0 0z1 They #irothord [he BALT providcd quantirativc informa hata drhterontdlbtnoution humanthan rdent Iunot In tnal tho Inphold colec llons Iion about thc airway Crten Mllg Iouna and Jumcn ratncr than Jubmucolr information about the qualanive (seu RESR-A Suf N oltnc 2srd 31" narute infammalory responsc CaLk 6). Howruer, [nEsC mcthods do distincuan cciuccnsmJM monocytcs Unich WeIc lymphocytes frozcn Talh than apidI thcy Incd DrOVde coroina Colebach J OIdet Jomeli make Incni information about lymphocyie subrypes morc quuablc Diffusing cpaciy Jorim (Dico) performed by 49? mnenoqlochemicl Ahthough 4udy Ihesc pa. the sinelc-brcath human UECGA sludice Ogilvic and co- MEnIS Cno ineinoo nad au*4i obyruction ways (7) uscd LoirCI' PK Morgan antibodics [0 mnonoclona s00'n] Kcic compired wuth 4utomaled dilfusing #nout obiain information ("FEV Morean; rJdicil infammalo: 8090) analyzcr (P aoout palicnis Challam UK cell (ypes Rent the cpithelium smokcd cigafetics the prcdicted uine L044ne Sub- ome qunng (ntlf valugs Irom but fivc 0f thc Crapo and Morris (I5). Mucosa Utvc t Iarger airways they did not conitolsubicei$ Jmj IQuI sample thc small bronchi Onne Obsinucico palicnts had soppcd smok. bronchi- Pathology thar arc responsiblc Ucis tesecion . Bctuusc COTOI Jiruay AIl sperimens wcn? obtaincd directly obstruction (3). PIeviMS (8) has shown Woik Iolal Irominc ccll counts do nol change alicr smoking e5" orraling room ad inflated with opiimal Cut- The present studics desiened [0 ieon- Leng cryocmbrdding alempi Tcunperalure number and Iypc cumnpaic Inc sedMJIC malcnat Inmlam (OC IMilcs Inc TcTl andee-smoker WeSME Elkhart . INI). marory cells Al polienis had Bl . exprcssion of histocompati: #hich was Council dilutcd s0o wth Medica bility quesuionnaire normal saling The infaled lob Wis anugens ministetsd locauon oblain occupalional TTadice aluminumt bronchial-associaled Toil and susnrnded abolt liquid lymphoid exposurc historics.and pulmonafy USSuC Tuncuan niiokEn (BALT) in Ihc Jungs of human smokers covcrcd styrofoam box for pcnormcd Ietahan a (Ict nttor #uth ainway obstruction with sutgety Ihc medid poftion Iuna Inose Latn - Spuometry obstruclion_ Itozen Ine lung 47 Thcy [urned 18^ dcgrees petlormed Dueeto curricd Collint on lung tissue resecied from patients (hilus downl andallowed computcnzed $PIrOme[er ( Warten Collins, sabove Inc I- with Bruntmc MA) The Percet ( prcdicicd periphcral lunR nlrogcn Ior another Inc Irozc tumors. which was loiccd Iad- (OFVC} lobc was sliced inio idly frozen shortly after Mlcapjol" YFE slces Using spailta andmidmalt resectco ripirilon (DMMFR} Mio" using and sudicd antibod- Fct[ monocionai calculaled accordine Ihe Tormula CTJ: qufnaie snecuc inflammaiory Poand collcagucs (91. Lung volumcsand ccll subtypes i- and (hc expressionofHLA (Rceroo Lolum runYGS Kinpiint ratmbc Class Kcie Class rE ICd Torn loimcd /Por> consiant-Dressur atecns; Jahlc Folume body plcthysmograph The rercent Pulmonary Niom Methoda nlrdicted rerIdua Reiraich volumie (# RV) was calcu- Laborutory; Unuetrsulv Britn Columonj Ialed according Goldman Tiuly Hotni conduciing hbonton iormulj Vancouygt. Dntih Columbis; Becklakc (10)_ Ongoing (inad of luna structure uo Ihc "TL( WJs 4i: Suncoueo and Tuncifonin#Ich Guani No according 4t7246 Itom Ihe GMacd palkenis rgquiting BJtes and 11t4 Retcjrh Councu cororkeis lung rescclion hatr tbcit Lunaorano lung (unction mcasured rsurg-volum Couna curiesofhe lung Hobncrn Ratramh-US; ptcopchalvciy wteconsirucicd lune 'perimcn Wicd ohe mcthod uccoloin ple tnondence Inniton uh TcduE4 Mrad and WultenbcrBer (121, andthc Picural cduni should bcaddicxrd formalit: Or glutaraldchvde Moua Jame} The Prescnt -Pul- Tonjty Rcaeatch Labogaiors St Paut;' pielsute InMztion IPmax) Mudi MaxImj baseo Junarom Hoia Hurrard Sirgel . ICncs nGcorded prrcctt predicicd ("oPmaw)ac: Vancouttt_R{ Canada V6z PUBL ICATIONS 016975
4479 Characterization of the Inilammatory Reaction in the Peripheral Airways of Cigarette Smokers Using Immunocytochemistry' -, FLE Licy ctr. Gaes CAROL BOSKEN JENNIFER HARDS, Kevin GATTER and Aizn~[ James HoCAc : Introduction Igarcitc smoking causcs JUI Inllamma Summart Lundliasue tom ?0*hents Unosnjoing totaction tory mcacon ving monoclnoi anllboolen tor #penphorlcaiinomtrs studiod Jirwals and Idonlln ~Ll detnnico tnaony Geli Non produccs airwzy obstruclion Eeentlonn oloncemncay Dationer Alrer  approxi acrording Anclatoolmphe iate (BLLT mately IS t0 20g0 0f Une pallenba hcav1 paneoniptoictodFev, (#LFFV ) Into amupad smokers (2). noerl obatnuclednFFy This obs[ruciion Tno Dlocled Iu7ge Wulled ett B01d1 Gnd Contro  (#Fv , locatcd the petiph- Ilourd nitroarnn Juuie cnembada allc #0 Inlc mtolulnuerTel Tcm lugutt] A_tror airways thoughi IO be c80 Lulno Ton 407101 hlatologk #uGIlo.na B4no L0t mplad ubIno com Dom innammatory pIOCES (4-6)- EpecIIc inIlde Irom (heto semolou Iuno blaintd wiln Monocionas mloncelityperehlch antlboclax Morphomctric studies ofthechronic Ji- tall ar rounicd Ten anonted O*pogiledpot BQu*7} Munimeto( amm way inflammation in smokers Inthe pntienla Wun nindy Aniat have bcen RovenIllla thon Jldth obalncuon nsd moro @-lymphonttt contrclJublacin based on classic histologic cxaminauon polmoraonuciear IoukooNar atalhenurt (nIed lo tho amouniumorid Jaupmuconal hax providcd quantirativc informa hata drhterontdlbtnoution humanthan 0 0z1 They #irothord [he BALT Iion about thc airway Mllg rdent Iunot In tnal tho Inphold colec llons and Jumcn Crten ratncr than Iouna Jubmucolr qualanive information about the (seu narute oltnc Suf N RESR-A infammalory responsc 2srd CaLk 31" 6). Howruer, [nEsC mcthods do distincuan cciuccnsmJM monocytcs lymphocytes Unich WeIc apidI frozcn Talh than thcy DrOVde OIdet Incd make Incni coroina morc quuablc Jomeli Colebach J information about lymphocyie subrypes Jorim mnenoqlochemicl Diffusing cpaciy 4udy (Dico) 49? Ihesc pa. performed by Ahthough UECGA sludice human MEnIS Cno the sinelc-brcath nad au*4i obyruction ineinoo Ogilvic and co- ways (7) uscd mnonoclona antibodics [0 s00'n] Kcic LoirCI' compired wuth PK Morgan #nout dilfusing 4utomaled obiain information aoout infammalo: ("FEV 8090) rJdicil palicnis analyzcr (P Morean; cell (ypes Challam the cpithelium smokcd cigafetics Rent UK Sub- ome L044ne qunng (ntlf uine the prcdicted Mucosa Utvc t but fivc 0f thc valugs Irom Iarger airways they did not conitolsubicei$ Jmj IQuI Crapo and Morris (I5). sample thc small bronchi Onne Obsinucico bronchi- palicnts had soppcd smok. thar arc responsiblc Ucis COTOI Pathology Jiruay tesecion . Bctuusc obstruction (3). PIeviMS Woik (8) has shown Iolal AIl sperimens wcn? obtaincd directly Irominc The present ccll counts do nol change alicr smoking e5" studics orraling room ad inflated with opiimal Cut- Leng desiened [0 ieon- cumnpaic Inc alempi sedMJIC Tcunperalure cryocmbrdding number and Iypc Inmlam (OC malcnat TcTl andee-smoker Al polienis had WeSME marory cells Bl . IMilcs Inc Elkhart . INI). exprcssion of histocompati: Medica Council #hich was quesuionnaire dilutcd s0o wth bility anugens normal saling locauon ministetsd oblain occupalional The infaled lob Wis TTadice aluminumt bronchial-associaled lymphoid USSuC exposurc historics.and pulmonafy Tuncuan Toil and susnrnded abolt liquid (BALT) in Ihc niiokEn Jungs of human smokers pcnormcd Ietahan nttor covcrcd styrofoam box for #uth ainway a (Ict obstruction with Inose sutgety Ihc medid Latn - poftion Iuna obstruclion_ Spuometry petlormed Itozen Thcy Dueeto curricd Collint Ine lung 47 [urned 18^ dcgrees on lung tissue resecied from patients computcnzed $PIrOme[er ( Warten (hilus downl andallowed with Collins, sabove Inc I- periphcral lunR Bruntmc MA) The Percet ( prcdicicd nlrogcn Ior another tumors. which was Iad- loiccd Inc Irozc idly frozen shortly after Mlcapjol" (OFVC} YFE lobc was sliced inio andmidmalt spailta slces Using resectco and sudicd ripirilon using Mio" (DMMFR} monocionai antibod- Fct[ calculaled accordine qufnaie snecuc Ihe Tormula CTJ: inflammaiory ccll subtypes Poand collcagucs (91. Lung volumcsand i- and (hc expressionofHLA Lolum runYGS (Rceroo Kcie Kinpiint Class ratmbc Class atecns; loimcd rE ICd Torn consiant-Dressur /Por> Folume Jahlc body plcthysmograph The rercent Methoda nlrdicted rerIdua Niom volumie (# RV) was calcu- Pulmonary Reiraich Unuetrsulv Laborutory; Ialed according Britn Columonj hbonton conduciing iormulj Goldman Tiuly Hotni Ongoing Vancouygt. uo Becklakc (10)_ Dntih Columbis; Ihc "TL( (inad of luna structure and Tuncifonin#Ich WJs 4i: Suncoueo GMacd according Guani No palkenis rgquiting BJtes and 11t4  4t7246 Itom Ihe lung rescclion hatr tbcit cororkeis Retcjrh Councu rsurg-volum Couna Lunaorano lung (unction mcasured ptcopchalvciy curiesofhe lung Hobncrn Ratramh-US; lune 'perimcn Wicd wteconsirucicd Inniton uh uccoloin ohe mcthod ple tnondence TcduE4 Mrad and WultenbcrBer (121, andthc Picural cduni formalit: Or glutaraldchvde should bcaddicxrd The Prescnt Jame} Moua pielsute -Pul- Mudi baseo Junarom MaxImj InMztion IPmax) Tonjty Rcaeatch Labogaiors St Paut;' ICncs nGcorded Hurrard Sirgel . Hoia prrcctt predicicd ("oPmaw)ac: Vancouttt_R{ Canada V6z PUBL ICATIONS 016975