(mi CIA (19851 60. 496 Trum Effect of specific anti-human leukaemia immunotoxins On the colony formation by human haematopoietic progenitors and by humnan leukacmia cells D. CHERVINSKY: TFBBI. K. SEON; Depar tetelt Fediatrics , Defaf IWn! Adokcsccuut Uintt aul Mulcular Iewnunologs , Mlenturial Institute , Ros Cark Bwffal. USA Knn (Acccptcd for publix cticm: Deceniher 1934 } SUMMARY The ? cific cyrotoxic aclivity cell leukaemia anli-human im)munotoxis ([T) war Invcstigaled lor (hieir cllccts on colony formation of Ieukaemic cells and nanmal hucmalopoictic projcnilors ic CFU-GM CFU-E und CFU-GEMM, These [T wcrc preparedl conjugating ricin A chain #ith lhe monoclonalantibodiesSNI andSN2 These anlibodics dcfine tw0 Wniyle huran Tcellleukaemia anligcns This study revcalsthar whik only marginall; Ihese normal poielic  progcnilors chiaioxil macmala humat strUnEY supprcss colom lormallan atet Thc Icukacna a supnrctioni auemcnlcd by the 10 mv NH,CI #hich results nrescncc Iolul >uppression_ Thc pfeSert esulis indicalc Iherapcutic uselulness of s IT for cradiculon Icukemix cclls (nc bone DlIfO# palrnls #ith ccll Icukacmija Polentially . such Mug Ze"d Done narowCn in aurologous transplantalion leukacmia patients Acieorde ricin chaim imumoloxns antibudics hunwan leukucmia mainociona CFU Ht INTRODUCTION Tumour cells specifically killed fixing: cytolytic anti-lurnour comorcuuicnl antibodies Complcte spccific eradicalion of Lumcur however beilc "Il he n accomplishcd b} using immunoloxins the covalcnt conjugales of anti-tumnour anibodics #ith wih loxin subunils (reviewed Olsnes _ Pihl, [992: Mcolen, Schreilxr Aat Tajdel. 1982; Youlc, Neville _ 1982; Raso, [982: Thoipe & Rossa 1982; Jansen &tel 1982; Vuteutaetl_19831 (" the {OxIns, diphtheria toxin aud is fragmcnts sudicd mijny Intc such Eor sludics have providcd much inlormation icmprtant underslunding thc mcchanism of ccll killing Uchida; 19824. However. Ulk: prevalence of anti-diphtheria toxin anlibodics Toxins icrchcd population zenders Uhis Ioxin and ils fragment Undesirable for clinical us . Morc nunion rccenlly . (hcrelore. much aItention has hecn given Ficin und &bfu and their planxns The ricin 4hunis molecuic caneshe [40 suhunits. chain. #hich are' linked by and disullide nond (retiewed Olsnes Pihl, 19821. Thc specilic for ctin Lcciin Whicn Eljclocc (e duce nTescni varicty Ofcells  The binding olinc Thc suriace ul#ta ricin Bchain t0 mamnialian e allon s thc cnry Ihe ricin chain into thc cylonlasm whcre chain Acl; on Uhc 60S subunit of tnc rbosone cause irrersible inhibitiun of protein synlhesis and cell dcath, [solatedricin E cell surfaces but % is non-toxic whilc isolaled ricin Chainhind cmtuin (RAnOI (unfesrondelicc jcon_ Demartnten Yolsuk Immunolog; , Roskell Far Ycnoris Elma StrerL; Bullzlo. Ncv Yurk [4261 USA neicnc 496 50567224
(mi CIA Trum (19851 60. 496 Effect of specific anti-human leukaemia immunotoxins On the colony formation by human haematopoietic progenitors and by humnan leukacmia cells TFBBI. D. CHERVINSKY: K. SEON; Depar tetelt Fediatrics , Adokcsccuut Uintt aul Defaf IWn! Mulcular Iewnunologs , Ros Cark Mlenturial Institute , Bwffal. Knn USA (Acccptcd for publix cticm: Deceniher 1934 } SUMMARY The ? cific cyrotoxic aclivity anli-human cell leukaemia im)munotoxis ([T) war Invcstigaled lor (hieir cllccts on colony formation of Ieukaemic cells and nanmal hucmalopoictic projcnilors ic CFU-GM CFU-E und CFU-GEMM, These [T wcrc preparedl conjugating ricin A chain #ith lhe monoclonalantibodiesSNI andSN2 These anlibodics dcfine tw0 Wniyle huran Tcellleukaemia anligcns This study revcalsthar whik Ihese only marginall; chiaioxil normal macmala poielic  progcnilors strUnEY supprcss colom lormallan humat Icukacna a Thc atet supnrctioni auemcnlcd by the nrescncc 10 mv NH,CI #hich results Iolul >uppression_ Thc pfeSert esulis indicalc Iherapcutic uselulness of s IT for cradiculon Icukemix cclls (nc bone DlIfO# palrnls #ith ccll Icukacmija Polentially . such Mug Ze"d Done narowCn in aurologous transplantalion leukacmia patients Acieorde imumoloxns ricin chaim mainociona antibudics hunwan leukucmia CFU Ht INTRODUCTION Tumour cells specifically killed comorcuuicnl fixing: cytolytic anti-lurnour antibodies Complcte spccific eradicalion of Lumcur n however "Il he beilc accomplishcd b} using immunoloxins the covalcnt conjugales of anti-tumnour anibodics #ith Aat wih loxin subunils (reviewed Olsnes _ Pihl, [992: Mcolen, Schreilxr Tajdel. 1982; Neville _ Youlc, 1982; Raso, [982: Thoipe & Rossa 1982; Jansen &tel 1982; Vuteutaetl_19831 (" the {OxIns, diphtheria toxin aud is fragmcnts sudicd mijny Intc Eor such sludics have providcd much inlormation icmprtant underslunding thc mcchanism of ccll killing Toxins icrchcd Uchida; 19824. However. Ulk: prevalence of anti-diphtheria toxin anlibodics nunion population zenders Uhis Ioxin and ils fragment Undesirable for clinical us . Morc rccenlly . (hcrelore. much aItention has hecn given planxns Ficin und &bfu and their 4hunis The ricin molecuic caneshe [40 suhunits. and chain. #hich are' linked by disullide nond (retiewed Olsnes Pihl, 19821. Thc ctin Lcciin Whicn specilic for Eljclocc (e duce nTescni Thc suriace ul#ta varicty Ofcells  The binding olinc ricin Bchain t0 mamnialian e allon s thc cnry Ihe ricin chain into thc cylonlasm whcre chain Acl; on Uhc 60S subunit of tnc rbosone cause irrersible inhibitiun of protein synlhesis and cell dcath, [solatedricin E Chainhind cell surfaces but % is non-toxic whilc isolaled ricin cmtuin (RAnOI (unfesrondelicc jcon_ Demartnten Yolsuk Immunolog; , Roskell Far Ycnoris neicnc Elma StrerL; Bullzlo. Ncv Yurk [4261 USA 496 50567224