t6 > , FILE COPY A*0 0i: 07 1untuer-e Ii- ~Eft:II Uarinkt ( #DEh 4Aiti AJii' Inhibition of Simian Virus 40 T-Antigen Expression by Cellular Differentiation ROBERT sco1T" Hinoo.CHINYUANTZEN AHD DAVID ESTERTIC. PARYz Aar Culorouuatia Vruan Lec tr HARI . Mau HntMml Pn Ceetatr Mucilu Aio Fal JartIcI Rccedicd Mateh 1X Eecld Aaemho amplosed Judt the edecis E somallc cli Murinc }TSt ~Ifmt GIk tram[ertod " il pSIntU Met 3 Thl: entruicntal epproech Wa; uiad betuuy Amtun `Irus J0 45140, T-anilarn eeprealon: dcmnibulian diferenilatlon >>atrin and Whe pSTJnao PLamid Nn odldcir JTJT &u lint ucll-chmatnitd LAI Gont nlalning Klrtne TEian Tethant naLrr cunlains Ihc cor} Tl-Iion Inr SWAcunntc Ana L mrdium- Rosl ol dhesr Gincnntatnlo slably Inizralcd p51 Jnco #hlch repraeerd onlitn Krrtcolnied Ahlllat cbiclcntic ulngrutt cunidiucrenthicd Poorl Hovrirt imn Fale Krtett markedly mprerstd adln ? dunrrcullallon Ihcx clones, LAXmnleen cacenctn T-antlecn rnraatun didnu Mestai Unom Tanleen rprcSslvn_ diacrnhialinn- PAt Tennthlon ediporytes that ! IcrmInol dlernlzllon cgGquU AdlMtreEni pruliltrathto pulrnlin] Au-ultd t grorih Induceu reaclitallor 08 crlls . nrollcrnlc- mstimulnllun 4nuu TeclmuAet Taanllgch eaproaslon W4;abo demonstruted durinx Jillenentlatlon ot SEw Tanlineneoniihn_ Rcnrctton Threr malatanblizh Thut IIce utort Mcshulur dnlereulAtlon T-antlucn-Immnrtalled humnam Rtrellnreter I4ndhinthlolocirul Hcmi Tauultxru turrelon rennu nSiInen-runelccled clon Anc Tmneen tlptetuur DutintIne Cnlcri~t] UudercouJitn numcrvichuneet 47 IunJ ftr murkedl; represed Junna udipre} tc Jullct . ceilultr Cent & Nai~iun (rV Snir Ween < WeM - anlcen *t ntencic Wncn Jinetcnal rrnc-~ion Ucn un crimin Iicht? Uaer I AenAtn Ae-th CSw} an 4mulnicd unu *7< Ihete udinutciIc " KgOU Aitt Jec Fmlricain AlM ElnE - ulIUI [erininal dullcrentiuliem Hdelin_Iac mndnn-Tcniiuleu emnbria, -cinjol Gclk44e Wnncnnl ctamplt Jurntlhc dhricnlijlicn crudenI cmrntoni Fcnc Kenerit aukcd uruuieni nnlec T-Jnufen-unmorialzed human Kcrlinorylc . Svjn cidc-cunij ricnlnncOLGnIC `utc poufciani conin Acanicrinl Aainn - AUATERIALS AD RIETHOnS Th Luleenrlken ininruca act ee Aler JDrrU Ihijcni channs IiAnalul ) cnc-i mescnchtMbi -cmcil LCI rulr munne utushl rern'-to nwle-Ul Wwnatn (7eand BAL Autial clona cel Hcn WAn Mte~eri Nte"t Mnisle Jukatentatitn Jerrhrd (16 %. 4 Leean Leir Wnn - Hrient JTAT c WUt1 Gurcu Tetenied Ceht WLe UcaetraeHecl ftr ~nccunt ccll Lunc- Unsortccico Uenvita hceue 4ela Is Jutu lerle mouilcu Faelc misdrm nct Equl wmcccn 176An citr !en_| :40 um'LJ( unenercnitnic4 [DVeteainIo fclal horinc tcrum (FASI AeuuaE J5i(tIe Fu nr nrintTC CnAML TCEI'Iu cuncuniluent DEntg Mcn Llant mxintinel noitomjtirui mbaan n Heneua SU40 Tannticen micunlamit- lee HLSY Gci pait Eenti~ prnait InaerIjlizad human ccll ine kinul Aeelunatatat Aatu Ihe Plcli; 4 HE-SV cell- Dutat Ie aanlani Ac[ culuned (cu alnl 4Cn 4het rmhiton MCDB 15} meuium Jng t#Tious colaclun AeuI nipITCMI Aum-t difletenlijlc Jnc thovche rnn"eenilt Iwuna. @at In WaGinI + rce-t Ant9t Ceenad ~ue_ITe a4 Vunieteni cinrcn? IraniIeculob kolutlon Eauusann Alaln ClUIATTI Ynamaca Yhi -ltn` Aa" #ctl heroncnuji Erttlini CLAI EniiFCn. at]L JCLu I .ml PB # DNA Pt Lnctr Ctz Antenninn Ihn-ecIcd #uhnstancuun) 4her unjulrienijkrv wQaIu Tanlme traL cenr nhophult Jcehniouc dell~, u-inf 4na Gicim In Tjcl rtnian 4nc Ybi @ene AUJit cahlc chra TUEU Mal unicprtuin Tatnill IiMa neJini failitlIne MmiuI anhinut @Ialadnanideu Faeu tkc4 h culdtumc Mer Fe Wuletenluutn -n M ull celk #cte utlaintna ttniu (41x Nti Rra Teytan Mun-Icelrd "cnircen #l] t Ueecnteu la Aenntenin-ncinc Cunalicnt Vatd Iha ,nubuasth Jlt ch  ed Jlnocict Jnu ~incig Wntncn analnt [ae 4n"i Watne Facni L4 nluecil- Flc Alued Inaun Iht zunie OlSnetanu cioncu WencieneNncFadl tcnLT I JAt micritthir mcinad de-cnu Llebuel A~inr cIl-nce"ce eieniticu Fter Winyt TnIr analtiduni ccller (6n, Wins - ucte@reu  Inm Uact DMEM conijining %05 FBS Icl eKhch Jullc(cnucdc (nt' eIant' ine] ul uunlcInC E enniect 4hol Ia ` J[ uuleeLHL Iu-mi Wqurn! <4u. 0I -at Fane Ee als Funci dcmtea Prctiutt nneauna un-Irclon #uh pltennd DNA Ae aeratctIc1 AIut; 5 Cleein Schlcuel Kanlt 4nleuIct(2i WnEIne&Juetit reften CMIuc .4ily" PUBL IcaTIONS 034366 0353
t6 > , FILE COPY A*0 0i: 1untuer-e Ii- 07 ~Eft:II ( #DEh 4Aiti AJii' Uarinkt Inhibition of Simian Virus 40 T-Antigen Expression by Cellular Differentiation DAVID ESTERTIC. PARYz Hinoo.CHINYUANTZEN AHD ROBERT sco1T" HntMml Pn Ceetatr Vruan Mau Lec tr HARI . Aar Culorouuatia Fal JartIcI Mucilu Aio Rccedicd Mateh 1X Eecld Aaemho Murinc }TSt ~Ifmt GIk tram[ertod " il pSIntU Met amplosed Judt the edecis E somallc cli dcmnibulian Thl: entruicntal epproech Wa; uiad betuuy Amtun `Irus J0 45140, T-anilarn eeprealon: JTJT &u lint ucll-chmatnitd Nn odldcir diferenilatlon >>atrin and Whe pSTJnao PLamid Klrtne naLrr TEian Tethant LAI Gont nlalning cunlains Ihc cor} Tl-Iion Inr SWAcunntc Ana slably Inizralcd p51 Jnco #hlch repraeerd onlitn Krrtcolnied Gincnntatnlo mrdium- Rosl ol dhesr cunidiucrenthicd Poorl Hovrirt Krtett imn Fale Ahlllat cbiclcntic ulngrutt Ihcx clones, LAXmnleen adln ? dunrrcullallon markedly mprerstd cacenctn Tanleen rprcSslvn_ diacrnhialinn- PAt Tennthlon T-antlecn rnraatun didnu Mestai Unom pruliltrathto pulrnlin] Au-ultd IcrmInol dlernlzllon cgGquU AdlMtreEni ediporytes that 4nuu TeclmuAet nrollcrnlc- crlls . mstimulnllun t grorih Induceu reaclitallor 08 Tanlineneoniihn_ Rcnrctton Taanllgch eaproaslon W4;abo demonstruted durinx Jillenentlatlon ot SEw T-antlucn-Immnrtalled humnam Rtrellnreter Threr malatanblizh Thut IIce utort Mcshulur dnlereulAtlon rennu Tauultxru turrelon I4ndhinthlolocirul Hcmi 3 L ! nSiInen-runelccled clon Anc Tmneen tlptetuur 47 IunJ ftr murkedl; represed Junna udipre} tc Jullct . Ae-th rrnc-~ion anlcen *t ntencic Wncn CSw} udinutciIc " an 4mulnicd KgOU unu *7< Ihete Wnncnnl [erininal dullcrentiuliem Hdelin_Iac Kenerit aukcd crudenI Jurntlhc dhricnlijlicn Svjn T-Jnufen-unmorialzed human Kcrlinorylc . DutintIne Cnlcri~t] UudercouJitn numcrvichuneet ceilultr Cent & Nai~iun (rV Snir Ween < WeM - Ucn un crimin Iicht? Uaer I AenAtn Jinetcnal ulIUI Aitt Jec Fmlricain AlM ElnE - ctamplt mndnn-Tcniiuleu emnbria, -cinjol Gclk44e uruuieni nnlec cmrntoni Fcnc ricnlnncOLGnIC `utc cidc-cunij conin poufciani Acanicrinl Aainn - ininruca act ee Aler Luleenrlken Th channs IiAnalul ) cnc-i JDrrU Ihijcni Autial utushl rern'-to nwle-Ul  Wwnatn Jukatentatitn Leean Leir Mte~eri Nte"t Mnisle Wnn - Tetenied Ceht WLe UcaetraeHecl ftr ~nccunt hceue 4ela Is Jutu lerle  citr !en_| :40 um'LJ( 176An unenercnitnic4 Fu nr J5i(tIe nrintTC CnAML TCEI'Iu noitomjtirui mbaan n Heneua Eenti~ prnait pait Dutat Ie  aanlani Ac[ Aatu Ihe Plcli; AeuI 4het nipITCMI rmhiton difletenlijlc Jnc thovche rnn"eenilt Iwuna. rce-t Ant9t Ceenad ~ue_ITe a4 Vunieteni Alaln ClUIATTI Ynamaca Yhi -ltn` Lnctr Ctz Antenninn at]L JCLu I .ml 4her traL cenr  unjulrienijkrv wQaIu Tanlme In Tjcl rtnian 4nc Ybi @ene AUJit anhinut @Ialadnanideu Faeu utlaintna ttniu Fe Wuletenluutn -n M ull "cnircen #l] t Mun-Icelrd Jlt ch  ed Vatd Iha ,nubuasth [ae 4n"i Watne Facni L4 tcnLT I JAt WencieneNncFadl eieniticu Fter Winyt TnIr cIl-nce"ce Icl eKhch Jullc(cnucdc (nt' eIant' ine] ul J[ uuleeLHL Iu-mi Ia ` Wqurn! <4u. Fane Ee als Funci AUATERIALS AD RIETHOnS rulr munne mescnchtMbi -cmcil LCI Hcn WAn BAL clona cel (7eand Gurcu c WUt1 Jerrhrd (16 %. 4 Hrient JTAT Unsortccico Uenvita ccll Lunc- nct Equl wmcccn mouilcu Faelc misdrm [DVeteainIo fclal horinc tcrum (FASI AeuuaE Llant mxintinel cuncuniluent DEntg Mcn micunlamit- lee HLSY Gci SU40 Tannticen InaerIjlizad human Aeelunatatat ccll ine kinul (cu alnl 4 HE-SV cell- 4Cn culuned Aum-t MCDB 15} meuium Jng t#Tious colaclun @at In WaGinI + IraniIeculob kolutlon Eauusann cinrcn? EniiFCn. heroncnuji Erttlini Aa" CLAI #ctl Ihn-ecIcd #uhnstancuun) PB # DNA Pt dell~, u-inf 4na Gicim nhophult Jcehniouc IiMa cahlc chra TUEU Mal unicprtuin Tatnill Mer tkc4 h culdtumc neJini failitlIne MmiuI (41x Nti Rra Teytan celk #cte la Aenntenin-ncinc Cunalicnt Ueecnteu analnt Jlnocict Jnu ~incig Wntncn nluecil- Flc Alued Inaun Iht zunie OlSnetanu cioncu A~inr micritthir mcinad de-cnu Llebuel (6n, Wins - ucte@reu  Inm Uact analtiduni ccller uunlcInC E enniect 4hol DMEM conijining %05 FBS 0I -at dcmtea Prctiutt nneauna Ae aeratctIc1 AIut; 5 un-Irclon #uh pltennd DNA Cleein WnEIne&Juetit reften Kanlt Schlcuel 4nleuIct(2i CMIuc .4ily" PUBL IcaTIONS 034366 0353