• CENTRIFUGATION (noun). The noun CENTRIFUGATION has 1 sense: 1. the process of separating substances by the use of a centrifuge. Familiarity information: CENTRIFUGATION used as a noun is very rare. CENTRIFUGATION (noun). The noun CENTRIFUGATION has 1 sense: 1. the process of separating substances by the use of a centrifuge. Familiarity information: CENTRIFUGATION used as a noun is very rare.