Live Oak, Florida. Live Oak is a city in Suwannee County, Florida, United States. The city is the county seat of Suwannee County and is located east of Tallahassee. As of 2010, the population recorded by the U.S. Census Bureau was 6,850. U.S. Highway 90, U.S. Highway 129 and Interstate 10 are major highways running through Live Oak.