Refrigerator Method. (Recommended). Frozen beef should not be defrosted at room temperature. Harmful bacteria may grow rapidly under such conditions. The best method for thawing beef (or any other type of meat) is to place it in the refrigerator where it will defrost slowly and safely.round Beef. (one pound bulk). Defrost in the refrigerator for: 24 hours. Additional Thawing and Defrosting Tips. 1  Beef that is not fully defrosted should not be cooked because the exterior of the beef may become overdone before the interior has had a chance to cook to the proper temperature.