How to prepare for a Pap test. Try to avoid having a Pap test during menstruation, or when you have your period. For best results, the test should be done in the middle of your cycle, 10–20 days after the first day of your menstrual period. Talk to your doctor or nurse if your appointment falls during your period. If your period is light, it may be possible to do the Pap test. Avoid having sexual intercourse for 24 hours before the test. Do not use a vaginal douche, vaginal medicines, tampons or contraceptive (spermicidal) creams, foams or gels (except as directed by your doctor) for 48 hours before the test.