RESULTS AND DISCUSSION The crude enzyme solution was extracted with distilled water from healthy radish roots. The outline and results of the purification of fl-amylase from radish roots are summarized in Table 1.Most of the active enzyme was found in the 100% saturation ammonium sulphate (11-2 U/mg, 89% recovery).he dependence of the activity of r-amylase from radish roots on the incubation time was linear up to 165 min. Also, the rate of the reaction of the enzyme was proportional to the increase in the amount of enzyme protein, up to 280 pg/reaction mixture.