Can anxiety cause testicle pain. Premium Questions. / discomfort and at base of penis and some testicle pain/ discomfort and kind of in behind my testicles most of the pain is dull but very noticeable. I feel it be affecting my bowel movements. But my anxiety and stress ... my left testicle hurts and I haven't hit puberty my right one did this once before please help ... I have been having testicle pain for last 24 hours with a slight itchy urination sensation. what could be the cause.