Transfer RNA plays a huge role in protein synthesis and translation. Its job is to translate the message within the nucleotide sequence of mRNA to a specific amino acid sequence. peptide bond forms connecting the amino acid of the tRNA in the P site to the amino acid of the tRNA in the A binding site. As the ribosome moves along the mRNA molecule, the tRNA in the P site is released and the tRNA in the A site is translocated to the P site.