However, we recommend a work concentration of 1-10 mg/ml. 1 to 10 column volumes are usually ideal. Up to 50 ml can be applied onto a 1 ml EndoTrap® column without loss of endotoxin removal efficiency (1 column volume “ready-to-use” = 1 ml). For EndoTrap®, HD we recommend a maximum LPS load of 2.5 x 106 EU/ml resin.