And without ovulation, you can’t have ovulation pain. Mid-cycle abdominal pain that is extreme or lasts longer than a day should be evaluated by a doctor. Appendicitis, ovarian cysts, and ectopic (tubal) pregnancy can sometimes mimic ovulation pain, although pain from these conditions is typically much more severe.he six signs of ovulation pain are: 1  It’s one-sided. 2  It comes on suddenly and without warning. 3  It’s a sharp pain, twinge, or cramping rather than a dull ache. 4  It often lasts only minutes, but may last a few hours or even up 24 hours.