The two most important hormones for maintaining calcium levels in the body are parathyroid hormone (PTH) and 1,25(OH)2D (the active form of vitamin D).The major regulator is PTH, which is part of a negative feedback loop to maintain [Ca ++ ] ECF (see Humoral regulation).he two most important hormones for maintaining calcium levels in the body are parathyroid hormone (PTH) and 1,25(OH)2D (the active form of vitamin D).