Average Cost of Pet Vaccinations. The cost for vaccinating puppies or pets in the first year ranges from $20 to $200. Cost of immunization of adult dogs ranges from $60 to $100 annually.Canine vaccination costs differ due to the dog's age and varying vet charges.Since puppies are at a high risk of exposure to disease, puppy vaccinations are recommended as early as 5 weeks of age.long with the cost of immunizations, pet owners are required to pay additional costs toward laboratory tests, physical examinations and routine parasite treatment or control. The cost of veterinary care for puppies ranges from $100 to $200. Veterinary care for older dogs costs nearly $150 to $250 annually.