Driving distance from Pensacola, FL to Destin, FL. The total driving distance from Pensacola, FL to Destin, FL is 48 miles or 77 kilometers. Your trip begins in Pensacola, Florida. It ends in Destin, Florida. If you are planning a road trip, you might also want to calculate the total driving time from Pensacola, FL to Destin, FL so you can see when you'll arrive at your destination.