Comparing Quotes. The total cost of air ambulance flights can range widely, from five digits all the way up to six in some rare cases. In general, a flight will be at least $10,000, but can cost more depending on the distance of the transport as well as any specialized staff and equipment that must be on board.Because air ambulance flights can cost so much, compare quotes from different air ambulances if you can.here exist a lot of variables that can affect the total price of an air ambulance flight. Nevertheless, while it is an important consideration for many people, price alone shouldn’t be the ultimate decision-maker when it comes to which air ambulance service you choose.