Hydration definition, any of a class of compounds containing chemically combined water. In the case of some hydrates, as washing soda, Na 2 CO 3 ⋅10H 2 O, the water is loosely held and is easily lost on heating; in others, as sulfuric acid, SO 3 ⋅H 2 O, or H 2 SO 4 , it is strongly held as water of constitution. to combine chemically with water.