ph ob ic ▁and ▁electro static ▁properties ▁are ▁highly ▁conser ved ▁in ▁this ▁mot if . ▁For ▁example , ▁a ▁local ▁hydro ph ob icity ▁maximum ▁occurs ▁in ▁the ▁vic inity ▁of ▁the ▁invariant ▁Le u ▁resid ue . ▁ ▁A ▁net ▁negative ▁charge ▁occurs ▁within ▁ 3 ▁resid ues ▁am ino - term inal ▁to ▁the ▁invariant ▁Le u ▁resid ue ; ▁further more , ▁posit ively ▁charged ▁am ino ▁ac ids ▁( L ys ▁or ▁Arg ) ▁are ▁not ▁found ▁within ▁the ▁Le u ▁– ▁x ▁– ▁C ys ▁– ▁x ▁– ▁G lu ▁sequence , ▁nor ▁in ▁the ▁positions ▁immediately ▁fl ank ing ▁this ▁sequence . ▁The ▁p R b - binding ▁mot if ▁and ▁neg atively ▁charged ▁region ▁match ▁to ▁a ▁segment ▁of ▁SV 4 0 ▁T Ag ▁beginning ▁at ▁resid ue ▁ 1 0 2 ▁and ▁ending ▁at ▁resid ue ▁ 1 1 5 ▁as ▁shown ▁below : ▁▁▁ ▁– ▁As n ▁– ▁Le u ▁– ▁P he ▁– ▁C ys ▁– ▁Ser ▁– ▁G lu ▁– ▁G lu ▁– ▁Met ▁– ▁Pro ▁– ▁Ser ▁– ▁Ser ▁– ▁Asp ▁– ▁Asp ▁– ▁G lu ▁– ▁ ▁Function al ▁studies ▁of ▁T Ag ▁prote ins ▁bearing ▁mut ations ▁within ▁this ▁segment ▁( am ino ▁acid ▁positions ▁ 1 0 6 ▁to ▁ 1 1 4 , ▁inclus ive ) ▁demonstrate ▁that ▁certain ▁de le ter ious ▁mut ations ▁abol ish ▁m align ant ▁transform ing ▁activity . ▁ ▁For ▁example , ▁mut ation ▁of ▁the ▁invariant ▁G lu ▁at