▁▁▁ ▁M N ase - seq ▁( M icro c oc cal ▁N uc lease ▁sequ encing ) ▁uses ▁the ▁micro c oc cal ▁nuc lease ▁en zym e ▁to ▁identify ▁nucle os ome ▁position ing ▁throughout ▁the ▁gen ome . ▁ ▁Ch rom os ome ▁con formation ▁capture ▁determ ines ▁the ▁spatial ▁organization ▁of ▁chrom atin ▁in ▁the ▁nucle us , ▁by ▁infer ring ▁genom ic ▁locations ▁that ▁phys ically ▁interact . ▁ ▁MAC C ▁profil ing ▁( M icro c oc cal ▁nuc lease ▁A CC ess ibility ▁profil ing ) ▁uses ▁tit r ation ▁series ▁of ▁chrom atin ▁dig ests ▁with ▁micro c oc cal ▁nuc lease ▁to ▁identify ▁chrom atin ▁access ibility ▁as ▁well ▁as ▁to ▁map ▁nucle os om es ▁and ▁non - h ist one ▁DNA - binding ▁prote ins ▁in ▁both ▁open ▁and ▁closed ▁regions ▁of ▁the ▁gen ome . ▁ ▁Ch rom atin ▁and ▁kn ots ▁ ▁It ▁has ▁been ▁a ▁puzz le ▁how ▁de cond ensed ▁inter phase ▁chrom os om es ▁remain ▁essentially ▁un k not ted . ▁The ▁natural ▁expectation ▁is ▁that ▁in ▁the ▁presence ▁of ▁type ▁II ▁DNA ▁to pois omer ases ▁that ▁permit ▁pass ages ▁of ▁double - str and ed ▁DNA ▁regions ▁through ▁each ▁other , ▁all ▁chrom os om es ▁should ▁reach ▁the ▁state ▁of ▁topological ▁equilibrium . ▁The ▁topological ▁equilibrium ▁in ▁highly ▁crow ded ▁inter phase ▁chrom os om es ▁forming ▁chrom os ome ▁territ ories ▁would ▁result ▁in ▁formation ▁of ▁highly ▁kn otted ▁chrom atin ▁fib