▁of ▁G S ▁requires ▁ 1 2 ▁ATP . ▁De aden y ly lation ▁by ▁AT ▁involves ▁ph osph or ol yt ic ▁removal ▁of ▁the ▁T yr - link ed ▁ad eny ly l ▁groups ▁as ▁AD P . ▁AT ▁activity ▁is ▁influenced ▁by ▁the ▁regul atory ▁protein ▁that ▁is ▁associated ▁with ▁it : ▁P II , ▁a ▁ 4 4 - k D ▁tr imer . ▁P II ▁also ▁under go es ▁post - trans l ational ▁modification ▁by ▁u rid y ly l ▁transfer ase , ▁thus ▁P II ▁has ▁two ▁forms . ▁The ▁state ▁of ▁P II ▁dict ates ▁the ▁activity ▁of ▁ad eny ly l ▁transfer ase . ▁If ▁P II ▁is ▁not ▁u rid y ly l ated , ▁then ▁it ▁will ▁take ▁on ▁the ▁P II A ▁form . ▁The ▁AT : PI IA ▁complex ▁will ▁de activ ate ▁G S ▁by ▁ad eny ly lation . ▁If ▁P II ▁is ▁u rid y ly l ated , ▁then ▁it ▁will ▁take ▁on ▁the ▁P I ID ▁form . ▁The ▁AT : PI ID ▁complex ▁will ▁activ ate ▁G S ▁by ▁de aden y ly lation . ▁The ▁AT : PI IA ▁and ▁AT : PI ID ▁complex es ▁are ▁al loster ically ▁reg ulated ▁in ▁a ▁reci pro cal ▁fashion ▁by ▁α - ket og l ut ar ate ▁( α - K G ) ▁and ▁gl ut am ine ▁( G ln ). ▁G ln ▁will ▁activ ate ▁AT : PI IA ▁activity ▁and