▁Run , ▁Wilson ▁Run , ▁and ▁Bull ▁Run . ▁Ludwig ▁Der r ▁built ▁a ▁mill ▁on ▁the ▁stream ▁in ▁the ▁ 1 7 0 0 s . ▁It ▁was ▁still ▁standing ▁in ▁ 1 8 6 0 . ▁ ▁Marcus ▁H uling ▁once ▁gained ▁a ▁tract ▁of ▁land ▁on ▁Lim estone ▁Run . ▁David ▁K ened y ▁purchased ▁ 2 0 0 ▁acres ▁of ▁land ▁on ▁Lim estone ▁Run ▁in ▁ 1 7 8 5 . ▁ ▁In ▁the ▁early ▁ 1 9 0 0 s , ▁some ▁residents ▁of ▁Lewis burg ▁dis charg ed ▁se w age ▁into ▁Lim estone ▁Run . ▁ ▁Lim estone ▁Run ▁flo oded ▁five ▁times ▁between ▁ 1 9 9 7 ▁and ▁ 2 0 1 2 . ▁In ▁ 2 0 1 2 , ▁workers ▁over seen ▁by ▁the ▁Union ▁County ▁Conserv ation ▁District ▁did ▁work ▁to ▁stabil ize ▁the ▁banks ▁of ▁Lim estone ▁Run ▁and ▁alter ed ▁the ▁channel ▁of ▁the ▁stream . ▁ ▁Bi ology ▁The ▁concentration ▁of ▁E . ▁col i ▁in ▁the ▁waters ▁of ▁Lim estone ▁Run ▁and ▁its ▁trib ut aries ▁ranges ▁from ▁ 9 0 ▁to ▁ 1 1 5 0 ▁colon ies ▁per ▁ 1 0 0 ▁mill il it ers . ▁The ▁areas ▁with ▁the ▁highest ▁concentration ▁of ▁E . ▁col i ▁also ▁have ▁high ▁concentr ations ▁of ▁alg ae . ▁ ▁Liv est ock ▁have ▁access ▁to ▁Lim estone ▁Run ▁in ▁its ▁upper ▁and ▁middle ▁reaches . ▁Canada ▁ge ese ▁and ▁wood ▁du cks ▁are ▁found ▁on