til is , ▁there ▁can ▁be ▁over ▁a ▁hundred ▁in ▁Stre pt omy ces ▁co el icol or . ▁Sign al ▁pe pt ides ▁that ▁can ▁recogn ise ▁the ▁Tat ▁prote ins ▁are ▁character ised ▁by ▁a ▁cons ensus ▁mot if ▁Ser / T hr - Arg - Arg - X - P he - Le u - L ys ▁( where ▁X ▁can ▁be ▁any ▁polar ▁am ino ▁acid ). ▁It ▁is ▁the ▁two ▁success ive ▁arg in ines ▁from ▁which ▁the ▁name ▁tw in ▁arg in ine ▁trans location ▁came ▁from . ▁Rep lacement ▁of ▁any ▁of ▁the ▁arg in ine ▁leads ▁to ▁slow ▁down ▁or ▁failure ▁of ▁secret ion . ▁ ▁Type ▁I ▁▁ ▁Type ▁I ▁secret ion ▁system ▁( T 1 SS ▁or ▁TO SS ) ▁is ▁found ▁in ▁Gram - negative ▁b acter ia . ▁It ▁depends ▁on ▁ch aper one ▁activity ▁using ▁H ly ▁and ▁Tol ▁prote ins . ▁The ▁system ▁activ ates ▁as ▁a ▁signal ▁sequence ▁H ly A ▁bind s ▁H ly B ▁on ▁the ▁cell ▁memb rane . ▁This ▁signal ▁sequence ▁is ▁an ▁ABC ▁trans porter . ▁The ▁H ly AB ▁complex ▁activ ates ▁H ly D ▁which ▁un co ils ▁and ▁moves ▁to ▁the ▁outer ▁cell ▁memb rane . ▁The ▁terminal ▁signal ▁is ▁recogn ised ▁by ▁Tol C ▁in ▁the ▁inner ▁memb rane . ▁The ▁H ly A ▁is ▁secret ed ▁out ▁of ▁the ▁outer ▁memb rane ▁through ▁a ▁tunnel - like ▁protein ▁channel . ▁ ▁T 1 SS ▁trans ports ▁various ▁mole