lication ▁to ▁ampl ify ▁small ▁fragments ▁of ▁genom ic ▁DNA ▁into ▁DNA ▁nan ob alls . ▁ ▁Fl u ores cent ▁nucle ot ides ▁bind ▁to ▁complement ary ▁nucle ot ides ▁and ▁are ▁then ▁polym er ized ▁to ▁anchor ▁sequences ▁bound ▁to ▁known ▁sequences ▁on ▁the ▁DNA ▁template . ▁ ▁The ▁base ▁order ▁is ▁determined ▁via ▁the ▁flu ores c ence ▁of ▁the ▁bound ▁nucle ot ides ▁This ▁DNA ▁sequ encing ▁method ▁allows ▁large ▁numbers ▁of ▁DNA ▁nan ob alls ▁to ▁be ▁sequ enced ▁per ▁run ▁at ▁lower ▁re agent ▁costs ▁compared ▁to ▁other ▁next ▁generation ▁sequ encing ▁platforms . ▁ ▁However , ▁a ▁limitation ▁of ▁this ▁method ▁is ▁that ▁it ▁generates ▁only ▁short ▁sequences ▁of ▁DNA , ▁which ▁presents ▁challeng es ▁to ▁mapping ▁its ▁reads ▁to ▁a ▁reference ▁gen ome . ▁After ▁purch asing ▁Complete ▁Gen om ics , ▁the ▁Be ij ing ▁Gen om ics ▁Institute ▁( B G I ) ▁ref ined ▁DNA ▁nan ob all ▁sequ encing ▁to ▁sequence ▁nucle ot ide ▁samples ▁on ▁their ▁own ▁platform . ▁ ▁Pro cedure ▁ ▁DNA ▁Nan ob all ▁Se qu encing ▁involves ▁isol ating ▁DNA ▁that ▁is ▁to ▁be ▁sequ enced , ▁she aring ▁it ▁into ▁small ▁ 1 0 0 ▁– ▁ 3 5 0 ▁base ▁pair ▁( bp ) ▁fragments , ▁lig ating ▁adapter ▁sequences ▁to ▁the ▁fragments , ▁and ▁circular izing ▁the ▁fragments . ▁ ▁The ▁circular ▁fragments ▁are ▁copied ▁by ▁rolling ▁circle ▁rep lication ▁resulting ▁in ▁many ▁single - str and ed ▁copies ▁of ▁each ▁fragment .