oph age ▁I 3 ▁and ▁role ▁of ▁DNA ▁g yr ase ▁in ▁my c ob acter ia ▁set ▁the ▁stage ▁for ▁his ▁later ▁work ▁when ▁he ▁joined ▁the ▁inst itute ▁as ▁an ▁assistant ▁professor ▁in ▁ 1 9 8 9 . ▁ ▁Research ▁ ▁Nag ar aja ▁has ▁done ▁research ▁on ▁DNA ▁to pois omer ases , ▁topology ▁mod ulation , ▁reg ulation ▁of ▁gene ▁expression ▁to ▁understand ▁the ▁underlying ▁mole cular ▁mechan isms ▁and ▁their ▁importance ▁in ▁cell ular ▁function . ▁ ▁Under standing ▁the ▁bi ology ▁of ▁the ▁path ogen ▁that ▁causes ▁T B ▁has ▁been ▁a ▁major ▁research ▁effort . ▁As ▁a ▁result , ▁several ▁sem inal ▁contributions ▁have ▁been ▁made ▁leading ▁to ▁potential ▁applications . ▁His ▁interaction ▁with ▁industry ▁includes ▁– ▁development ▁of ▁commercial ▁bi ote ch ▁products ▁such ▁as ▁restriction ▁en zym es ▁and ▁other ▁DNA ▁transaction ▁en zym es , ▁design ▁and ▁development ▁of ▁high ▁f idel ity ▁restriction ▁en zym e , ▁new ▁in hib itors ▁for ▁the ▁generation ▁of ▁lead ▁mole cules ▁as ▁a ▁step ▁for ▁novel ▁th era pe ut ics ▁against ▁t uber cul osis . ▁These ▁have ▁been ▁successfully ▁applied ▁and ▁resulted ▁in ▁international ▁pat ents . ▁Under ▁his ▁guidance , ▁ 2 1 ▁students ▁have ▁completed ▁doctor al ▁degree ▁and ▁ 1 0 ▁students ▁are ▁at ▁various ▁stages ▁of ▁their ▁Ph D . ▁ ▁In ▁addition , ▁a ▁large ▁number ▁of ▁project ▁assist ants , ▁summer ▁fell ows ▁and ▁post do ctor al ▁research ers ▁are ▁trained ▁under ▁him . ▁