▁region ▁that ▁is ▁ampl ified ▁by ▁PC R ▁also ▁has ▁certain ▁base ▁orders ▁that ▁make ▁it ▁sus cept ible ▁to ▁be ▁dig ested ▁by ▁H pa II ▁( or ▁H ha I ) ▁en zym e ▁when ▁it ▁is ▁not ▁m eth yl ated . ▁This ▁detail ▁gives ▁the ▁opportunity ▁to ▁research ers ▁to ▁differenti ate ▁a ▁m eth yl ated ▁alle le ▁from ▁the ▁un m eth yl ated ▁one . ▁ ▁Thanks ▁to ▁these ▁qual ities ▁of ▁H U MA RA ▁gene , ▁cl onal ▁origin ▁of ▁any ▁t issue ▁from ▁a ▁female ▁m amm al ian ▁organ ism ▁can ▁be ▁determined . ▁ ▁The ▁basic ▁process ▁is ▁performed ▁as ▁the ▁following ▁: ▁▁ 1 -) ▁DNA ▁from ▁the ▁t issue ▁is ▁isolated . ▁▁ 2 -) ▁The ▁isolated ▁DNA ▁is ▁treated ▁with ▁the ▁suitable ▁en zym e ▁( such ▁as ▁H pa II ) ▁in ▁optimal ▁conditions ▁for ▁a ▁suggested ▁amount ▁of ▁time ▁( i . e . ▁over night ). ▁▁ 3 -) ▁DNA ▁is ▁clean ed ▁and ▁the ▁certain ▁region ▁of ▁H U MA RA ▁gene ▁is ▁ampl ified ▁by ▁PC R ▁using ▁" su itable " ▁prim ers ▁( as ▁an ▁example , ▁please ▁see : Ref . 2 ) ▁▁ 4 -) ▁After ▁running ▁PC R ▁products ▁through ▁a ▁gel , ▁the ▁gel ▁is ▁visual ized ▁and ▁the ▁results ▁are ▁analyz ed ▁accordingly . ▁If ▁two ▁bands ▁are ▁apparent , ▁the ▁t issue ▁studied ▁is ▁most ▁likely ▁of ▁poly cl onal ▁origin . ▁If ▁a ▁single