▁ant ib od ies ▁from ▁character ised ▁al log ene ic ▁ant is era ▁or ▁mon oc l onal ▁ant ib od ies . ▁These ▁ant ib od ies ▁are ▁inc ub ated ▁one ▁by ▁one ▁with ▁patient ‘ s ▁or ▁don or ‘ s ▁l ym ph ocy tes ▁and ▁source ▁of ▁complement . ▁Am ount ▁of ▁dead ▁cells ▁( and ▁thus ▁positive ▁result ) ▁is ▁measured ▁by ▁dead ▁or ▁live ▁cells ▁st aining . ▁Now ad ays ▁CD C ▁typing ▁is ▁being ▁replaced ▁by ▁mole cular ▁typing , ▁which ▁can ▁identify ▁nucle ot ide ▁sequences ▁of ▁H LA ▁mole cules ▁via ▁PC R . ▁ ▁CD C ▁ass ay ▁is ▁usually ▁used ▁for ▁performing ▁cross match ▁test . ▁The ▁basic ▁version ▁involves ▁inc ub ation ▁of ▁patient ’ s ▁ser um ▁with ▁don or ’ s ▁l ym ph ocy tes ▁and ▁second ▁inc ub ation ▁after ▁adding ▁rabb it ▁complement . ▁Pres ence ▁of ▁dead ▁cell ▁( pos itive ▁test ) ▁means ▁that ▁don or ▁isn ‘ t ▁suitable ▁for ▁this ▁particular ▁patient . ▁There ▁are ▁modifications ▁available ▁to ▁increase ▁test ▁sens itivity ▁including ▁extension ▁of ▁minimal ▁inc ub ation ▁time , ▁adding ▁anti human ▁glob ul in ▁( A H G ), ▁removing ▁un bound ▁ant ib od ies ▁before ▁adding ▁complement , ▁separation ▁of ▁T ▁cell ▁and ▁B ▁cell ▁subset . ▁Besides ▁CD C ▁cross match ▁there ▁is ▁  flow - cy t ometric ▁cross match ▁available , ▁that ▁is ▁more ▁sensitive ▁and ▁can ▁detect ▁even ▁complement ▁non