ase ▁has ▁been ▁identified , ▁which ▁trans ports ▁c ob alt ▁across ▁the ▁cell ▁memb rane . ▁The ▁identity ▁of ▁the ▁metal ▁in ▁the ▁active ▁site ▁of ▁a ▁nit rile ▁hyd rat ase ▁can ▁be ▁predicted ▁by ▁analysis ▁of ▁the ▁sequence ▁data ▁of ▁the ▁alpha ▁sub unit ▁in ▁the ▁region ▁where ▁the ▁metal ▁is ▁bound . ▁The ▁presence ▁of ▁the ▁am ino ▁acid ▁sequence ▁V CT LC ▁indicates ▁a ▁Co - cent red ▁N H ase ▁and ▁the ▁presence ▁of ▁V CS LC ▁indicates ▁Fe - cent red ▁N H ase . ▁ ▁Met abol ic ▁path way ▁N it rile ▁hyd rat ase ▁and ▁amid ase ▁are ▁two ▁hyd rating ▁and ▁hydro ly t ic ▁en zym es ▁responsible ▁for ▁the ▁sequ ential ▁met abol ism ▁of ▁nit ril es ▁in ▁b acter ia ▁that ▁are ▁capable ▁of ▁util ising ▁nit ril es ▁as ▁their ▁sole ▁source ▁of ▁nit ro gen ▁and ▁carbon , ▁and ▁in ▁concert ▁act ▁as ▁an ▁alternative ▁to ▁nit ril ase ▁activity , ▁which ▁performs ▁nit rile ▁hydro ly s is ▁without ▁formation ▁of ▁an ▁intermediate ▁primary ▁am ide . ▁A ▁sequence ▁in ▁gen ome ▁of ▁the ▁cho an of lag ell ate ▁Mon os iga ▁bre vic oll is ▁was ▁suggested ▁to ▁encode ▁for ▁a ▁nit rile ▁hyd rat ase . ▁The ▁M . ▁bre vic oll is ▁gene ▁consisted ▁of ▁both ▁the ▁alpha ▁and ▁beta ▁sub un its ▁f used ▁into ▁a ▁single ▁gene . ▁Similar ▁nit rile ▁hyd rat ase ▁gen es ▁consisting ▁of ▁a