▁solution , ▁which ▁would ▁kill ▁most ▁other ▁micro - organ isms . ▁In ▁this ▁case , ▁the ▁expression ▁system ▁is ▁cheap ▁to ▁set ▁up ▁and ▁maintain . P ich ia ▁can ▁grow ▁u o ▁to ▁a ▁very ▁high ▁cell ▁density . ▁Under ▁ideal ▁conditions , ▁it ▁can ▁multiply ▁to ▁the ▁point ▁where ▁the ▁cell ▁susp ension ▁is ▁pract ically ▁a ▁paste . ▁As ▁the ▁protein ▁yield ▁from ▁expression ▁system ▁in ▁a ▁micro be ▁is ▁roughly ▁equal ▁to ▁the ▁product ▁of ▁the ▁prote ins ▁produced ▁per ▁cell , ▁which ▁makes ▁P ich ia ▁of ▁great ▁use ▁when ▁trying ▁to ▁produce ▁large ▁quantities ▁of ▁protein ▁without ▁expensive ▁equipment . ▁ ▁Compar ing ▁to ▁other ▁expression ▁systems , ▁such ▁as ▁S 2 - cell s ▁from ▁D ros oph ila ▁mel an og aster ▁and ▁Chinese ▁ham ster ▁ov ary ▁cells , ▁P ich ia ▁usually ▁gives ▁much ▁better ▁yields . Gener ally , ▁cell ▁lines ▁from ▁mult ic ell ular ▁organ isms ▁require ▁complex ▁and ▁expensive ▁types ▁of ▁media , ▁including ▁am ino ▁ac ids , ▁vit am ins , ▁as ▁well ▁as ▁other ▁growth ▁factors . ▁These ▁types ▁of ▁media ▁significantly ▁increase ▁the ▁cost ▁of ▁producing ▁heter olog ous ▁prote ins . ▁Additionally , ▁since ▁P ich ia ▁can ▁grow ▁in ▁media ▁containing ▁only ▁one ▁carbon ▁source ▁and ▁one ▁nit ro gen ▁source , ▁which ▁is ▁suitable ▁for ▁is ot op ic ▁lab elling ▁applications , ▁like ▁protein ▁N MR . ▁ ▁Indust rial ▁applications P . ▁past oris ▁have ▁been ▁used ▁in ▁several