- 2 3 ▁between ▁Cal la han , ▁and ▁Fol k ston , ▁Georgia . ▁ ▁History ▁ ▁US ▁ 3 0 1 ▁first ▁entered ▁Florida ▁in ▁ 1 9 4 9 , ▁termin ating ▁in ▁T ampa . ▁ ▁It ▁was ▁r er out ed ▁along ▁the ▁' old ▁US ▁ 4 1 ' ▁route ▁to ▁Pal met to ▁in ▁ 1 9 5 2 , ▁by pass ing ▁T ampa , ▁and ▁extended ▁in ▁ 1 9 5 3 ▁to ▁its ▁current ▁southern ▁termin us ▁of ▁Sar as ota . ▁ ▁From ▁ 1 9 5 6 ▁until ▁ 1 9 9 3 , ▁US ▁ 3 0 1 ▁signs ▁in ▁Florida ▁featured ▁black ▁number ing ▁on ▁a ▁yellow ▁shield . ▁The ▁" color - c oding " ▁of ▁U . S . ▁Rout es ▁by ▁the ▁Florida ▁Department ▁of ▁Transport ation ▁was ▁stopped ▁when ▁the ▁state ▁could ▁no ▁longer ▁use ▁Federal ▁funds ▁to ▁replace ▁the ▁signs ▁with ▁anything ▁but ▁the ▁standard ▁black - and - white ▁version . ▁Some ▁yellow ▁US ▁ 3 0 1 ▁signs ▁may ▁still ▁remain . ▁ ▁Ar ound ▁ 1 9 8 5 , ▁U . S . ▁ 3 0 1 ▁was ▁real igned ▁in ▁southern ▁Man ate e ▁County ▁between ▁the ▁Sar as ota ▁County ▁Line ▁and ▁U . S . ▁ 4 1 ▁to ▁its ▁current ▁alignment ▁along ▁a ▁former ▁Atlantic ▁Coast ▁Line ▁Rail road ▁cor rid or . ▁ ▁U . S . ▁ 3 0 1 ▁previously ▁ran ▁to ▁the