▁that ▁followed , ▁the ▁research ers ▁worked ▁to ▁ref ine ▁the ▁extract s ▁to ▁a ▁degree ▁safe ▁for ▁human ▁injection ▁with ▁the ▁help ▁of ▁bio chem ist ▁James ▁Col lip . ▁T ensions ▁mounted ▁during ▁this ▁time ▁between ▁the ▁four ▁" co - dis co vers " ▁of ▁ins ul in , ▁ex ac erb ated ▁greatly ▁by ▁Col lip ' s ▁suggestion ▁that ▁he ▁could ▁return ▁any time ▁to ▁Al berta ▁with ▁his ▁pur ification ▁work ▁and ▁pat ent ▁it . ▁Fitz G er ald ▁therefore ▁stepped ▁in ▁as ▁pe ac em aker ▁to ▁prepare ▁a ▁sem inal ▁research ▁and ▁development ▁agreement ▁between ▁the ▁Con n aught ▁Labor ator ies ▁and ▁the ▁research ers . ▁It ▁established ▁two ▁key ▁conditions : ▁ 1 ) ▁that ▁the ▁collabor ators ▁would ▁sign ▁a ▁contract ▁agree ing ▁not ▁to ▁take ▁out ▁a ▁pat ent ▁with ▁a ▁commercial ▁ph arma ce ut ical ▁firm ▁during ▁an ▁initial ▁working ▁period ▁with ▁Con n aught ; ▁and ▁ 2 ) ▁that ▁no ▁changes ▁in ▁the ▁research ▁policy ▁would ▁be ▁allowed ▁unless ▁first ▁discussed ▁among ▁Fitz G er ald ▁and ▁the ▁four ▁collabor ators . ▁ ▁As ▁clin ical ▁tri als ▁progress ed ▁through ▁ 1 9 2 2 , ▁a ▁number ▁of ▁early ▁patients ▁benef itted ▁from ▁the ▁develop ments , ▁including ▁Leonard ▁Thompson ▁at ▁the ▁Toronto ▁General ▁Hospital , ▁Elizabeth ▁Hugh es ▁G os sett ▁( d augh ter ▁of ▁U . S . ▁Secretary ▁of ▁State ▁Charles ▁Evans ▁Hugh es ), ▁and ▁future ▁wood cut ▁artist