▁same ▁sequence ▁led ▁to ▁a ▁conclusion ▁that ▁s perm ▁m t D NA ▁has ▁mut ations ▁that ▁cause ▁low ▁s perm ▁mob ility . ▁Another ▁example ▁would ▁be ▁the ▁recent ▁report ▁describing ▁a ▁heter op las mic ▁m t D NA ▁mole c ule ▁containing ▁five ▁linked ▁miss ense ▁mut ations ▁dispers ed ▁over ▁the ▁cont igu ous ▁m t D NA ▁CO 1 ▁and ▁CO 2 ▁gen es ▁in ▁Al z heimer ’ s ▁disease ▁patients , ▁however , ▁the ▁more ▁recent ▁studies ▁using ▁PC R , ▁restriction ▁end on uc lease ▁site ▁variant ▁ass ays , ▁and ▁ph y log en ic ▁analysis ▁proposed ▁that ▁the ▁nuclear ▁CO 1 ▁and ▁CO 2 ▁sequences ▁revealed ▁that ▁they ▁diver ged ▁from ▁modern ▁human ▁m t DN As ▁early ▁in ▁hom in id ▁evolution ▁about ▁ 7 7 0 , 0 0 0 ▁years ▁before ▁and ▁these ▁preserved ▁NUM T s ▁could ▁cause ▁Al z heimer ' s ▁disease . ▁ ▁One ▁of ▁the ▁possible ▁ways ▁of ▁prevent ing ▁from ▁such ▁err one ous ▁result ▁is ▁an ▁ampl ification ▁and ▁comparison ▁of ▁heter ogeneous ▁sequence , ▁compr ises ▁both ▁m t D NA ▁and ▁n D NA , ▁with ▁the ▁obtained ▁results ▁from ▁S anger ▁sequ encing ▁of ▁pur ified ▁and ▁en rich ed ▁m t D NA ▁as ▁shown ▁in ▁figure ▁ 4 . ▁Although ▁this ▁method ▁is ▁easy ▁and ▁only ▁a ▁few ▁prim ers ▁are ▁required , ▁it ▁will ▁prevent ▁from ▁a ▁substantial ▁error ▁in ▁ph y log en etic ▁studies ▁of