▁that ▁can ▁be ▁anal ys ed ▁for ▁C TC s ▁( approx . ▁ 7 5 0 ▁m l ▁in ▁ 3 0 ▁min ▁compared ▁to ▁ 7 . 5 ▁m l ▁of ▁a ▁drawn ▁blood ▁sample ). ▁ ▁Cell Search ▁method ▁Cell Search ▁is ▁the ▁only ▁F DA - appro ved ▁platform ▁for ▁C TC ▁isol ation . ▁This ▁method ▁is ▁based ▁on ▁the ▁use ▁of ▁iron ▁nan op articles ▁co ated ▁with ▁a ▁polym er ▁layer ▁carrying ▁bi ot in ▁analog ues ▁and ▁conjug ated ▁with ▁ant ib od ies ▁against ▁Ep C AM ▁for ▁the ▁capture ▁of ▁C TC s . ▁Is olation ▁is ▁couple d ▁to ▁an ▁analy zer ▁to ▁take ▁images ▁of ▁isolated ▁cells ▁upon ▁their ▁st aining ▁with ▁specific ▁flu ores cent ▁ant ib ody ▁conjug ates . ▁Blood ▁is ▁sample d ▁in ▁an ▁ED TA ▁t ube ▁with ▁an ▁added ▁pres erv ative . ▁Upon ▁arrival ▁in ▁the ▁lab , ▁ 7 . 5 m L ▁of ▁blood ▁is ▁cent rif ug ed ▁and ▁placed ▁in ▁a ▁prepar ation ▁system . ▁This ▁system ▁first ▁en rich es ▁the ▁tum or ▁cells ▁imm un om agnet ically ▁by ▁means ▁of ▁ferro fl uid ▁nan op articles ▁and ▁a ▁magnet . ▁Sub sequently , ▁recovered ▁cells ▁are ▁per me abil ized ▁and ▁st ained ▁with ▁a ▁nuclear ▁st ain , ▁a ▁flu ores cent ▁ant ib ody ▁conjug ate ▁against ▁CD 4 5 ▁( le uk ocy te ▁marker ) ▁and ▁cy to ker at ins ▁ 8