- specific ▁chemical ▁c ue ▁that ▁initi ates ▁recognition ▁and ▁inc ub ation ▁of ▁a ▁bro od ▁cl ump , ▁as ▁opposed ▁to ▁individual ▁body ▁od or ▁or ▁ph er om one ▁signals . ▁ <0x09> ▁These ▁c ues , ▁however , ▁do ▁not ▁appear ▁to ▁be ▁derived ▁from ▁the ▁actual ▁bro od ▁cl ump . ▁Instead , ▁it ▁appears ▁that ▁during ▁egg - lay ing , ▁the ▁que ens ▁depos it ▁some ▁sort ▁of ▁signal ▁at ▁the ▁site ▁of ▁the ▁nest . ▁It ▁is ▁possible ▁that ▁they ▁continu ously ▁depos it ▁or ▁" ren ew " ▁the ▁signal ▁over ▁time . ▁The ▁signal ▁must ▁be ▁recogn izable ▁by ▁the ▁queen ▁and ▁by ▁the ▁workers ▁be es ▁who ▁will ▁eventually ▁assist ▁the ▁queen ▁in ▁inc ub ation ▁and ▁bro od - re aring ▁after ▁the ▁first ▁generation ▁is ▁m ature d . ▁ ▁Queen ▁contribution ▁ ▁The ▁queen ▁of ▁this ▁be e ▁species ▁has ▁been ▁known ▁to ▁contribute ▁to ▁for aging ▁efforts . ▁During ▁the ▁early ▁season , ▁when ▁the ▁queen ▁is ▁young , ▁she ▁primarily ▁focus es ▁on ▁bro od ▁inc ub ation . ▁However , ▁towards ▁the ▁end ▁of ▁the ▁season ▁as ▁worker ▁numbers ▁decl ine , ▁the ▁queen ▁becomes ▁a ▁significant ▁contrib utor ▁to ▁for aging ▁efforts . ▁They ▁go ▁so ▁far ▁as ▁to ▁become ▁the ▁primary ▁pol len ▁and ▁ne ct ar ▁prov iders ▁for ▁the ▁col ony . ▁The ▁mechan isms ▁behind ▁this ▁behavior al ▁change ▁are ▁not ▁well ▁understood , ▁but ▁significance ▁has ▁been ▁attributed ▁to ▁careful