▁band ▁is ▁observed , ▁the ▁t issue ▁is ▁mon oc l onal ▁unless ▁two ▁alle les ▁have ▁exactly ▁the ▁same ▁numbers ▁of ▁C AG ▁repe ats ▁or ▁different ▁cells ▁with ▁the ▁same ▁in activ ated ▁initi ated ▁the ▁tum or ; ▁so , ▁seem ingly ▁mon oc l onal ▁although ▁it ▁is ▁actually ▁poly cl onal . ▁ ▁In ▁order ▁to ▁make ▁a ▁conclusion ▁about ▁the ▁cl on ality ▁of ▁a ▁tum or , ▁it ▁is ▁best ▁to ▁use ▁the ▁DNA ▁from ▁a ▁normal ▁t issue ▁of ▁the ▁same ▁person , ▁and ▁a ▁sample ▁without ▁en zym e ▁treatment ▁must ▁also ▁be ▁ampl ified ▁as ▁a ▁control . ▁If , ▁even ▁in ▁normal ▁t issues ▁without ▁en zym e ▁treatment , ▁a ▁single ▁band ▁is ▁observed , ▁it ▁may ▁be ▁explained ▁as ▁follows ; ▁this ▁person ▁has ▁the ▁gen etic ▁pattern ▁as ▁X O ▁( this ▁possibility ▁can ▁be ▁ex cluded ▁to ▁see ▁a ▁band ▁after ▁en zym e ▁treatment ▁because , ▁if ▁indeed ▁X O ▁is ▁the ▁gen etic ▁pattern ▁of ▁the ▁sample , ▁then , ▁there ▁will ▁be ▁NO ▁m eth yl ation , ▁therefore ▁no ▁band ▁should ▁be ▁visible ▁after ▁dig est ing ▁with ▁the ▁en zym e . ▁In ▁the ▁case ▁of ▁seeing ▁a ▁band ▁after ▁en zym e ▁treatment , ▁the ▁observation ▁is ▁most ▁likely ▁to ▁mean ▁that ▁the ▁person ▁has ▁two ▁X ▁chrom os om es ▁with ▁exact ▁C AG ▁repe ats .) ▁When ▁you ▁see ▁two ▁bands ▁for ▁normal ▁t issue ▁( both ▁en zym e