ated ▁to ▁see ▁band ing ▁pattern ▁and ▁weight ▁changes ▁that ▁may ▁have ▁occurred ▁post ▁translation . ▁C 5 orf 5 0 ▁Poly cl onal ▁Ant ib ody ▁from ▁Th ermo F isher ▁Scient ific ▁has ▁a ▁Western ▁Bl ot ▁band ing ▁pattern ▁at ▁ 4 0 ▁k Da . ▁This ▁predict s ▁that ▁there ▁is ▁a ▁significant ▁amount ▁of ▁post - trans l ational ▁modification ▁by ▁addition ▁of ▁large ▁components . ▁ ▁There ▁are ▁many ▁ph osph ory lation ▁sites ▁along ▁its ▁sequence ▁including ▁two ▁protein ▁kin ase ▁C ▁ph osph ory lation ▁sites , ▁c AMP - ▁and ▁c G MP - dependent ▁protein ▁kin ase ▁ph osph ory lation ▁site , ▁and ▁a ▁ty ros ine ▁kin ase ▁ph osph ory lation ▁site . ▁There ▁is ▁also ▁a ▁confident ▁potential ▁C - term inal ▁G PI - Mod ification ▁Site . ▁ ▁Second ary ▁structure ▁ ▁There ▁are ▁two ▁st ret ches ▁of ▁alpha ▁hel ices ▁from ▁am ino ▁acid ▁ 3 3 ▁to ▁ 4 9 ▁and ▁ 8 9 ▁to ▁ 1 3 6 ▁based ▁on ▁evidence ▁from ▁various ▁programs ▁that ▁predict ▁secondary ▁structure . ▁The ▁most ▁inform ative ▁of ▁all ▁the ▁programs ▁from ▁the ▁ones ▁investig ated ▁is ▁P E LE ▁on ▁Bi ology ▁Work ben ch . ▁ ▁A ▁ 3 D ▁protein ▁structure ▁was ▁predicted ▁to ▁look ▁like ▁a ▁series ▁of ▁hel ices , ▁similar ▁to ▁what ▁was ▁predicted ▁by ▁other ▁programs . ▁ ▁Sub cell ular ▁local ization ▁ ▁This ▁human ▁integral ▁memb rane ▁protein