i otic ▁ass ay , ▁depending ▁on ▁the ▁rep orter ▁gene ▁used ▁in ▁the ▁expression ▁vector . ▁ ▁Single - cell ▁library ▁prepar ation ▁▁ ▁After ▁successfully ▁trans du ced ▁cells ▁have ▁been ▁selected ▁for , ▁isol ation ▁of ▁single ▁cells ▁is ▁needed ▁to ▁conduct ▁sc R NA - seq . ▁P ert urb - seq ▁and ▁C ROP - seq ▁have ▁been ▁performed ▁using ▁dro ple t - based ▁technology ▁for ▁single ▁cell ▁isol ation , ▁while ▁the ▁closely ▁related ▁CR IS P - seq ▁was ▁performed ▁with ▁a ▁mic row ell - based ▁approach . ▁Once ▁cells ▁have ▁been ▁isolated ▁at ▁the ▁single ▁cell ▁level , ▁reverse ▁trans cription , ▁ampl ification ▁and ▁sequ encing ▁takes ▁place ▁to ▁produce ▁gene ▁expression ▁profiles ▁for ▁each ▁cell . ▁Many ▁sc R NA - seq ▁approaches ▁incorpor ate ▁unique ▁mole cular ▁ident ifiers ▁( UM Is ) ▁and ▁cell ▁bar codes ▁during ▁the ▁reverse ▁trans cription ▁step ▁to ▁index ▁individual ▁R NA ▁mole cules ▁and ▁cells , ▁respectively . ▁These ▁additional ▁bar codes ▁serve ▁to ▁help ▁quant ify ▁R NA ▁trans cript s ▁and ▁to ▁associate ▁each ▁of ▁the ▁sequences ▁with ▁their ▁cell ▁of ▁origin . ▁ ▁Bio in format ics ▁analysis ▁▁ ▁Read ▁alignment ▁and ▁processing ▁are ▁performed ▁to ▁map ▁quality ▁reads ▁to ▁a ▁reference ▁gen ome . ▁De con volution ▁of ▁cell ▁bar codes , ▁guide ▁bar codes ▁and ▁U M Is ▁enables ▁the ▁association ▁of ▁guide ▁R N As ▁with ▁the ▁cells ▁that ▁contain ▁them , ▁thus ▁allowing ▁the ▁gene ▁expression