▁construct , ▁mut agen esis , ▁in ▁a ▁s ill ico ▁analysis ▁of ▁A CS ▁mot ifs , ▁and ▁pl as mid ▁loss ▁rate ▁ass ay ▁found ▁that ▁mig rat ory ▁mit och ond rial ▁D N As ▁can ▁impact ▁the ▁rep lication ▁of ▁the ▁nuclear ▁region ▁in ▁which ▁they ▁are ▁inserted . ▁Through ▁their ▁functional ▁evidence , ▁they ▁showed ▁that ▁sequences ▁of ▁mit och ond rial ▁origin ▁promote ▁n D NA ▁rep lication ▁in ▁Sac char omy ces ▁c ere vis iae ▁. ▁NUM T s ▁are ▁rich ▁ 1 1 - bp ▁AR S ▁core - A ▁cons ensus ▁sequence ▁( AC S ), ▁which ▁its ▁presence ▁in ▁the ▁matches ▁to ▁these ▁cons ensus ▁mot ifs , ▁in ▁the ▁Sac char omy ces ▁c ere vis iae ▁origin ▁of ▁rep lication , ▁is ▁necessary ▁but ▁not ▁sufficient ▁for ▁the ▁function ▁of ▁rep lication ▁origin ▁and ▁any ▁mut ation ▁in ▁this ▁cons ensus ▁causes ▁the ▁reduction ▁or ▁loss ▁of ▁DNA ▁rep lication ▁activity . ▁Given ▁the ▁high ▁density ▁of ▁A CS ▁mot ifs , ▁some ▁NUM T s ▁appear ▁essentially ▁as ▁A CS ▁carri ers . ▁In ▁contrast , ▁rep lication ▁efficiency ▁is ▁higher ▁in ▁those ▁ye ast ▁stra ins ▁that ▁have ▁pl asm ids ▁containing ▁both ▁NUM T ▁and ▁AR S . ▁They ▁also ▁found ▁that ▁some ▁NUM T s ▁can ▁work ▁as ▁an ▁independent ▁rep lication ▁fork ▁and ▁late ▁chrom os om al ▁orig ins ▁and ▁NUM T s ▁located ▁close ▁to ▁or ▁within ▁AR S ▁provide ▁key ▁sequence ▁elements