▁use ▁of ▁a ▁higher - str ength ▁g elling ▁agent ▁may ▁reduce ▁the ▁risk ▁from ▁hyper h yd ric ity . ▁Hyper h yd ric ity ▁can ▁also ▁be ▁controlled ▁by ▁bottom ▁cool ing , ▁which ▁allows ▁water ▁to ▁con den se ▁on ▁the ▁medium , ▁the ▁use ▁of ▁cy to kin in - meta - top olin ▁( 6 -( 3 - H ydro xy ben zy lam ino ) pur ine ) </ 9 >, ▁the ▁combination ▁of ▁lower ▁cy to kin in ▁and ▁am mon ium ▁nit rate ▁in ▁the ▁medium , ▁use ▁of ▁nit rate ▁or ▁gl ut am ine ▁as ▁the ▁sole ▁nit ro gen ▁source ▁ ▁and ▁decre asing ▁the ▁ratio ▁of ▁N H 4 + : NO 3 - ▁in ▁the ▁medium . ▁In ▁studies ▁on ▁calci um ▁def ic iency ▁in ▁t issue ▁cult ures ▁of ▁Lav and ula ▁ang ust ifolia , ▁it ▁was ▁shown ▁that ▁an ▁increase ▁in ▁calci um ▁in ▁the ▁medium ▁reduced ▁hyper h yd ric ity . ▁ ▁See ▁also ▁ ▁Call us ▁( cell ▁bi ology ) ▁ ▁Ch im era ▁( gen et ics ) ▁ ▁Som atic ▁emb ry ogen esis ▁ ▁Emb ry o ▁rescue ▁ ▁Notes ▁and ▁references ▁ ▁External ▁links ▁ ▁http :// users . ug ent . be /~ p de berg h / t ro / t ro 3 _ d 0 1 . htm ▁ ▁Category : Cell ▁culture ▁Category : Bi ote chn ology ▁Category : Cell ▁bi ology ▁Category