▁figure ▁on ▁right , ▁which ▁is ▁the ▁secondary ▁structure ▁of ▁lead zym e ▁generated ▁using ▁m fold . ▁The ▁structures ▁of ▁lead zym e ▁have ▁also ▁been ▁solved ▁using ▁X - ray ▁cry stal log raph y ▁and ▁N MR . ▁The ▁cry stal ▁structures ▁of ▁the ▁two ▁conform ations ▁of ▁lead zym e ▁are ▁shown ▁in ▁the ▁lower ▁figure ▁on ▁right . ▁ ▁Catal yt ic ▁mechanism ▁of ▁lead zym e ▁ ▁Le ad zym e ▁is ▁thought ▁to ▁perform ▁catal ysis ▁using ▁a ▁two - step ▁mechanism . ▁In ▁the ▁first ▁step ▁of ▁the ▁reaction , ▁the ▁ph osph odi ester ▁bond ▁is ▁cle aved ▁into ▁two ▁products : ▁ 5 ’ ▁product ▁termin ating ▁in ▁ 2 ’ 3 ’ ▁cy clic ▁ph osph ate ▁and ▁the ▁ 3 ’ ▁product ▁in ▁ 5 ’ ▁hydro x yl . ▁This ▁step ▁is ▁similar ▁to ▁other ▁small ▁self - cle aving ▁rib oz ym es ▁such ▁as ▁the ▁Ham mer head ▁rib oz yme ▁and ▁HD V ▁rib oz yme . ▁Both ▁of ▁those ▁rib oz ym es ▁generate ▁a ▁product , ▁which ▁contain ▁a ▁ 2 ’ , ▁ 3 ’ ▁- cyc lic ▁ph osph ate . ▁However , ▁in ▁lead zym e ▁this ▁product ▁is ▁just ▁an ▁intermediate . ▁In ▁the ▁second ▁step ▁of ▁this ▁reaction ▁path way , ▁the ▁ 2 ’ ▁ 3 ’ ▁- cyc lic ▁ph osph ate ▁under go es ▁hydro ly s is ▁to ▁form ▁ 3 ’ ▁mon oph osph ate