▁Single - Cell ▁CR IS PR ▁Screen ing ▁Platform ▁En ables ▁System atic ▁Dis section ▁of ▁the ▁Un fold ed ▁Prote in ▁Response ” ▁the ▁research ers ▁suppress ed ▁multiple ▁gen es ▁in ▁each ▁cell ▁to ▁study ▁the ▁unfold ed ▁protein ▁response ▁( UP R ) ▁path way . ▁With ▁a ▁similar ▁method ology , ▁but ▁using ▁the ▁term ▁CR IS P - seq ▁instead ▁of ▁P ert urb - seq , ▁the ▁paper ▁" Dis sect ing ▁Im m une ▁Circ uits ▁by ▁Link ing ▁CR IS PR - P oo led ▁S creens ▁with ▁Single - Cell ▁R NA - Seq " ▁performed ▁a ▁proof ▁of ▁concept ▁experiment ▁by ▁using ▁the ▁technique ▁to ▁pro be ▁regul atory ▁path ways ▁related ▁to ▁inn ate ▁imm unity ▁in ▁m ice . ▁L eth ality ▁of ▁each ▁perturb ation ▁and ▁ep istas is ▁anal ys es ▁in ▁cells ▁with ▁multiple ▁perturb ations ▁was ▁also ▁investig ated ▁in ▁these ▁papers . ▁P ert urb - seq ▁has ▁so ▁far ▁been ▁used ▁with ▁very ▁few ▁perturb ations ▁per ▁experiment , ▁but ▁it ▁can ▁theoret ically ▁be ▁scale d ▁up ▁to ▁address ▁the ▁whole ▁gen ome . ▁Finally , ▁the ▁October ▁ 2 0 1 6 ▁pre print ▁and ▁subsequent ▁paper ▁demonstrate ▁the ▁bio in form atic ▁reconst ruction ▁of ▁the ▁T ▁cell ▁re ceptor ▁signal ing ▁path way ▁in ▁Jur k at ▁cells ▁based ▁on ▁C ROP - seq ▁data . ▁ ▁While ▁these ▁publications ▁used ▁these ▁protocol s ▁for ▁answering ▁complex ▁bi ological ▁questions ,