0 1 3 ▁- ▁December ▁ 3 1 , ▁ 2 0 1 7 ▁ ▁Initial ▁en roll ment ▁into ▁the ▁W I HS ▁occurred ▁between ▁October ▁ 1 9 9 4 ▁and ▁November ▁ 1 9 9 5 . ▁The ▁total ▁initial ▁en roll ment ▁for ▁the ▁W I HS ▁was ▁ 2 , 0 5 6 ▁H IV - pos itive ▁women ▁and ▁ 5 6 9 ▁H IV - negative ▁women . ▁ ▁Since ▁W I HS ▁rec ru ited ▁its ▁initial ▁population , ▁several ▁new ▁tr ends ▁developed ▁as ▁the ▁H IV ▁ep ide mic ▁evol ved , ▁all ▁of ▁which ▁argued ▁for ▁an ▁expansion ▁of ▁the ▁W I HS ▁coh ort . ▁First , ▁despite ▁careful ▁follow - up ▁of ▁the ▁coh ort , ▁the ▁in ev itable ▁course ▁of ▁ill ness ▁and ▁time ▁had ▁led ▁to ▁attr ition ▁and ▁death . ▁ ▁Additionally , ▁increased ▁sample ▁sizes ▁were ▁needed ▁because ▁the ▁effect iveness ▁and ▁consequences ▁of ▁High ly ▁Active ▁Ant ire t ro vir al ▁Ther apy ▁( HA ART ) ▁added ▁new ▁str ata ▁to ▁many ▁key ▁anal ys es , ▁and ▁decre ased ▁the ▁inc idence ▁of ▁clin ical ▁out comes . ▁And ▁finally , ▁as ▁the ▁original ▁coh ort ▁continued ▁to ▁age , ▁it ▁became ▁less ▁able ▁to ▁support ▁studies ▁of ▁risk ▁behav iors , ▁sex ually ▁transm itted ▁dise ases , ▁and ▁re product ive ▁function . ▁Thus , ▁W I HS ▁fund ing ▁was ▁augment ed ▁in ▁ 2 0 0 1