▁the ▁lig ation ▁point ▁results ▁in ▁improved ▁lig ation . ▁ ▁Dis adv antages ▁ ▁The ▁main ▁dis adv antage ▁of ▁DNA ▁nan ob all ▁sequ encing ▁is ▁the ▁short ▁read ▁length ▁of ▁the ▁DNA ▁sequences ▁obtained ▁with ▁this ▁method . ▁Short ▁reads , ▁especially ▁for ▁DNA ▁high ▁in ▁DNA ▁repe ats , ▁may ▁map ▁to ▁two ▁or ▁more ▁regions ▁of ▁the ▁reference ▁gen ome . ▁ ▁A ▁second ▁dis adv antage ▁of ▁this ▁method ▁is ▁that ▁multiple ▁r ounds ▁of ▁PC R ▁have ▁to ▁be ▁used . ▁ ▁This ▁can ▁introduce ▁PC R ▁bias ▁and ▁possibly ▁ampl ify ▁cont amin ants ▁in ▁the ▁template ▁construction ▁phase . ▁However , ▁these ▁dis adv antages ▁are ▁common ▁to ▁all ▁short - read ▁sequ encing ▁platforms ▁are ▁not ▁specific ▁to ▁DNA ▁nan ob alls . ▁ ▁App lications ▁ ▁DNA ▁nan ob all ▁sequ encing ▁has ▁been ▁used ▁in ▁recent ▁studies . ▁ ▁Lee ▁et ▁al . ▁used ▁this ▁technology ▁to ▁find ▁mut ations ▁that ▁were ▁present ▁in ▁a ▁lung ▁cancer ▁and ▁compared ▁them ▁to ▁normal ▁lung ▁t issue . ▁They ▁were ▁able ▁to ▁identify ▁over ▁ 5 0 , 0 0 0 ▁single ▁nucle ot ide ▁variants . ▁Ro ach ▁et ▁al . ▁used ▁DNA ▁nan ob all ▁sequ encing ▁to ▁sequence ▁the ▁genom es ▁of ▁a ▁family ▁of ▁four ▁relatives ▁and ▁were ▁able ▁to ▁identify ▁SN P s ▁that ▁may ▁be ▁responsible ▁for ▁a ▁M end elian ▁dis order , ▁and ▁were ▁able ▁to ▁estimate ▁the ▁inter - gener ation ▁mut ation ▁rate