, ▁combined ▁with ▁the ▁presence ▁of ▁therm oph iles ▁in ▁the ▁genus , ▁has ▁led ▁to ▁the ▁development ▁of ▁a ▁variety ▁of ▁new ▁commercial ▁en zym e ▁products ▁with ▁the ▁desired ▁temperature , ▁p H ▁activity , ▁and ▁stability ▁properties ▁to ▁address ▁a ▁variety ▁of ▁specific ▁applications . ▁Class ical ▁mut ation ▁and ▁( or ) ▁selection ▁techniques , ▁together ▁with ▁advanced ▁cl oning ▁and ▁protein ▁engineering ▁strateg ies , ▁have ▁been ▁explo ited ▁to ▁develop ▁these ▁products . ▁E ff ort s ▁to ▁produce ▁and ▁se crete ▁high ▁yields ▁of ▁foreign ▁recom bin ant ▁prote ins ▁in ▁Bac ill us ▁hosts ▁initially ▁appeared ▁to ▁be ▁ham per ed ▁by ▁the ▁de grad ation ▁of ▁the ▁products ▁by ▁the ▁host ▁prote ases . ▁Rec ent ▁studies ▁have ▁revealed ▁that ▁the ▁slow ▁fol ding ▁of ▁heter olog ous ▁prote ins ▁at ▁the ▁memb rane - cell ▁wall ▁interface ▁of ▁Gram - pos itive ▁b acter ia ▁rend ers ▁them ▁vulner able ▁to ▁attack ▁by ▁wall - associ ated ▁prote ases . ▁In ▁addition , ▁the ▁presence ▁of ▁th i ol - dis ul ph ide ▁ox id ored uct ases ▁in ▁B . ▁sub til is ▁may ▁be ▁benef icial ▁in ▁the ▁secret ion ▁of ▁dis ul ph ide - b ond - cont aining ▁prote ins . ▁Such ▁develop ments ▁from ▁our ▁understanding ▁of ▁the ▁complex ▁protein ▁trans location ▁mach in ery ▁of ▁Gram - pos itive ▁b acter ia ▁should ▁allow ▁the ▁resolution ▁of ▁current ▁secret ion ▁challeng es ▁and ▁make ▁Bac