ot ation . ▁ ▁Single ▁cell ▁sorting ▁ ▁Single ▁cell ▁sorting ▁provides ▁a ▁method ▁for ▁sorting ▁a ▁heter ogeneous ▁mixture ▁of ▁cells ▁based ▁upon ▁int rac ell ular ▁and ▁ext rac ell ular ▁properties . ▁Single ▁cell ▁methods ▁allow ▁us ▁to ▁understand ▁cell ular ▁properties ▁that ▁can ▁be ▁sometimes ▁obsc ured ▁or ▁non ▁evident ▁with ▁populations ▁of ▁cells . ▁There ▁are ▁several ▁methods ▁for ▁sorting ▁single ▁cells : ▁ ▁The ▁mic ror aft ▁array ▁provides ▁a ▁rapid , ▁cost - effect ive ▁method ▁for ▁isol ating ▁cells , ▁analyz ing ▁cells ▁over ▁time , ▁and ▁generating ▁cl onal ▁populations ▁with ▁the ▁unique ▁ability ▁to ▁monitor ▁all ▁int ra - ▁and ▁ext rac ell ular ▁properties . ▁This ▁system ▁is ▁ideal ▁for ▁both ▁ad her ent ▁and ▁non - ad her ent ▁cell ▁types . ▁ ▁A ▁single ▁cell ▁method ▁to ▁observe ▁the ▁response ▁to ▁an ▁external ▁stim ulus , ▁in ▁this ▁case , ▁cell ular ▁response ▁to ▁a ▁lig and ▁was ▁studied ▁using ▁a ▁micro fl uid ic ▁device ▁with ▁micro ▁channel ▁val ves ▁to ▁trap ▁a ▁single ▁cell ▁into ▁a ▁chamber . ▁A ▁ 2 3 ▁val ve ▁system ▁was ▁used ▁for ▁actu ation ▁and ▁flu ores cent ▁d ye ▁was ▁used ▁stim ulus ▁response ▁imag ing . ▁ ▁Single ▁cell ▁clust ering ▁methods ▁are ▁a ▁series ▁of ▁statistical ▁methods ▁designed ▁by ▁data ▁scient ists ▁based ▁on ▁int rac ell ular ▁properties . ▁The ▁whole ▁process ▁includes ▁Single ▁Cell ▁R NA - Seq ▁data ▁gather ing , ▁Data ▁pre processing ▁for ▁clust