▁M ole cular ▁analysis ▁is ▁usually ▁required ▁for ▁an ▁accurate ▁diagn osis . ▁ ▁Di agnostics ▁The ▁prefer able ▁method ▁for ▁diagn osis ▁of ▁P . ▁mal aria e ▁is ▁through ▁the ▁exam ination ▁of ▁peri pher al ▁blood ▁films ▁st ained ▁with ▁Gi ems a ▁st ain . ▁PC R ▁techniques ▁are ▁also ▁commonly ▁used ▁for ▁diagn oses ▁confirm ation ▁as ▁well ▁as ▁to ▁separate ▁mixed ▁Pl as mod ium ▁in fe ctions . ▁Even ▁with ▁these ▁techniques , ▁however , ▁it ▁may ▁still ▁be ▁impossible ▁to ▁differenti ate ▁in fe ctions , ▁as ▁is ▁the ▁case ▁in ▁areas ▁of ▁South ▁America ▁where ▁humans ▁and ▁mon keys ▁co exist ▁and ▁P . ▁mal aria e ▁and ▁P . ▁bras ilian um ▁are ▁not ▁easily ▁distinguish able . ▁ ▁Bi ology ▁ ▁Life ▁cycle ▁ ▁P . ▁mal aria e ▁is ▁the ▁only ▁human ▁mal aria ▁par as ite ▁that ▁causes ▁fe vers ▁that ▁rec ur ▁at ▁approximately ▁three - day ▁intervals ▁( there fore ▁occurr ing ▁every ▁fourth ▁day , ▁a ▁quart an ▁fe ver ), ▁longer ▁than ▁the ▁two - day ▁( ter t ian ) ▁intervals ▁of ▁the ▁other ▁mal arial ▁par as ites . ▁ ▁Human ▁in fection ▁ ▁Liver ▁stage ▁In ▁this ▁stage , ▁many ▁thousands ▁of ▁m ero zo ites ▁are ▁produced ▁in ▁each ▁sch izont . ▁As ▁the ▁m ero zo ites ▁are ▁released , ▁they ▁in va de ▁er yth ro cy tes ▁and ▁initi ate ▁the ▁er yth ro cy t ic ▁cycle ,