▁and ▁certain ▁alg ae ▁( rh od oph y tes , ▁crypt omon ads , ▁gla uc oc yst oph y tes ) ▁which ▁capture ▁light ▁energy , ▁which ▁is ▁then ▁passed ▁on ▁to ▁ch lor oph yll s ▁during ▁photos yn thesis . ▁Ph yc obil ip rote ins ▁are ▁formed ▁of ▁a ▁complex ▁between ▁prote ins ▁and ▁ ▁c oval ently ▁bound ▁ph yc obil ins ▁that ▁act ▁as ▁chrom op hor es ▁( the ▁light - capt uring ▁part ). ▁ ▁They ▁are ▁most ▁important ▁constitu ents ▁of ▁the ▁ph yc obil is om es . ▁ ▁Major ▁ph yc obil ip rote ins ▁ ▁Character istics ▁and ▁applications ▁in ▁bi ote chn ology ▁ ▁Ph yc obil ip rote ins ▁el ic it ▁great ▁flu ores cent ▁properties ▁compared ▁to ▁small ▁organ ic ▁flu or op hor es , ▁especially ▁when ▁high ▁sens itivity ▁or ▁mult icol or ▁detection ▁is ▁required ▁: ▁ ▁Broad ▁and ▁high ▁absor ption ▁of ▁light ▁su its ▁many ▁light ▁sources ▁ ▁Very ▁inten se ▁emission ▁of ▁light : ▁ 1 0 - 2 0 ▁times ▁br ighter ▁than ▁small ▁organ ic ▁flu or op hor es ▁ ▁Rel ative ▁large ▁Sto kes ▁shift ▁gives ▁low ▁background , ▁and ▁allows ▁mult icol or ▁det e ctions . ▁ ▁Ex c itation ▁and ▁emission ▁spect ra ▁do ▁not ▁overlap ▁compared ▁to ▁conventional ▁organ ic ▁d yes . ▁ ▁Can ▁be ▁used ▁in ▁tan dem ▁( sim ult aneous ▁use ▁by ▁F RE T ) ▁with ▁conventional