u ency , ▁the ▁percentage ▁of ▁the ▁growth ▁surface ▁covered ▁by ▁cells . ▁The ▁cells ▁will ▁often ▁have ▁a ▁preferred ▁range ▁of ▁confl uen cies ▁for ▁optimal ▁growth , ▁for ▁example ▁a ▁m amm al ian ▁cell ▁line ▁like ▁He La ▁or ▁Raw ▁ 2 6 4 . 7 ▁generally ▁prefer ▁confl uen cies ▁over ▁ 1 0 % ▁but ▁under ▁ 1 0 0 %, ▁and ▁sub c ulture ▁will ▁normally ▁try ▁to ▁keep ▁the ▁cells ▁in ▁this ▁range . ▁For ▁sub c ulture ▁cells ▁may ▁be ▁det ached ▁by ▁one ▁of ▁several ▁methods ▁including ▁try ps in ▁treatment ▁to ▁break ▁down ▁the ▁prote ins ▁responsible ▁for ▁surface ▁ad her ence , ▁ch el ating ▁calci um ▁ ions ▁with ▁ED TA ▁which ▁dis rupt s ▁some ▁protein ▁ad her ence ▁mechan isms , ▁or ▁mechanical ▁methods ▁like ▁repeated ▁was hing ▁or ▁use ▁of ▁a ▁cell ▁scra per . ▁The ▁det ached ▁cells ▁are ▁then ▁res us p ended ▁in ▁fresh ▁growth ▁medium ▁and ▁allowed ▁to ▁sett le ▁back ▁onto ▁their ▁growth ▁surface . ▁ ▁See ▁also ▁Try ps in ization ▁ ▁References ▁ ▁Category : M icro bi ology ▁terms <0x0A> </s> ▁Lin na em ya ▁is ▁a ▁genus ▁of ▁f lies ▁in ▁the ▁family ▁T ach in idae . ▁ ▁Species ▁ ▁L . ▁alta ica ▁Rich ter , ▁ 1 9 7 9 ▁L . ▁amb igua ▁Sh ima , ▁ 1 9 8 6 ▁L . ▁at ri vent ris ▁( Mal lo ch , ▁ 1 9