▁easier ▁for ▁the ▁en zym e ▁to ▁lose ▁more . ▁ ▁In ▁the ▁sequ ential ▁model ▁there ▁are ▁many ▁different ▁global ▁conform ational / energy ▁states . ▁Binding ▁of ▁one ▁lig and ▁changes ▁the ▁en zym e ▁so ▁it ▁can ▁bind ▁more ▁lig ands ▁more ▁easily , ▁i . e . ▁every ▁time ▁it ▁bind s ▁a ▁lig and ▁it ▁wants ▁to ▁bind ▁another ▁one . ▁ ▁Ne ither ▁model ▁fully ▁explains ▁al loster ic ▁binding , ▁however . ▁ ▁The ▁recent ▁combined ▁use ▁of ▁physical ▁techniques ▁( for ▁example , ▁x - ray ▁cry stal log raph y ▁and ▁solution ▁small ▁angle ▁x - ray ▁scatter ing ▁or ▁S AX S ) ▁and ▁gen etic ▁techniques ▁( site - direct ed ▁mut agen esis ▁or ▁SD M ) ▁may ▁improve ▁our ▁understanding ▁of ▁al lost ery . ▁ ▁References ▁ ▁External ▁links ▁ ▁Bio chem istry ▁ 6 th ▁Ed , ▁S try er ▁Berg ▁and ▁T ym ocz ko ▁ ▁Category : En zym es <0x0A> </s> ▁Gian fran co ▁Gan au ▁( born ▁ 3 ▁March ▁ 1 9 5 5 ) ▁is ▁an ▁Italian ▁medic ▁and ▁politician , ▁Mayor ▁of ▁S ass ari ▁from ▁ 2 0 0 5 ▁to ▁ 2 0 1 4 . ▁ ▁Biography ▁Gr adu ated ▁in ▁Medicine ▁at ▁the ▁University ▁of ▁S ass ari , ▁Gan au ▁began ▁his ▁career ▁as ▁a ▁card i ologist ▁and ▁became ▁responsible ▁of ▁the ▁emer gency ▁medical ▁services ▁in ▁the ▁province ▁of ▁S ass ari ▁and ▁the