▁and ▁S cc 4 ▁are ▁both ▁required ▁for ▁coh es in ▁to ▁load ▁in ▁bud ding ▁ye ast . ▁ ▁Local ization ▁of ▁coh es in ▁rings ▁ ▁C oh es in ▁binding ▁along ▁the ▁chrom os om al ▁DNA ▁is ▁considered ▁to ▁be ▁dynamic ▁and ▁its ▁location ▁changes ▁based ▁on ▁gene ▁trans cription , ▁specific ▁DNA ▁sequence ▁and ▁presence ▁of ▁chrom os ome - associ ated ▁prote ins . ▁There ▁are ▁three ▁possible ▁scenarios : ▁▁ ▁C oh es in ▁location ▁is ▁influenced ▁by ▁the ▁orientation ▁of ▁neighbor ing ▁gen es ▁and ▁it ▁is ▁most ▁frequently ▁located ▁in ▁areas ▁of ▁conver gent ▁trans cription . ▁Gene ▁orientation ▁depends ▁on ▁the ▁direction ▁of ▁trans cription ▁and ▁can ▁be ▁of ▁three ▁types : ▁head - to - head , ▁head - to - tail ▁and ▁tail - to - tail . ▁The ▁tail - to - tail ▁configuration ▁results ▁in ▁the ▁convergence ▁of ▁trans cription ▁mach in ery . ▁One ▁hypothesis ▁states ▁that ▁the ▁R NA ▁polym er ase ▁“ push es ” ▁coh es in ▁along ▁the ▁DNA , ▁causing ▁them ▁to ▁move ▁towards ▁the ▁direction ▁of ▁the ▁R NA ▁polym er ases . ▁Ch anging ▁the ▁trans cription ▁pattern ▁of ▁gen es ▁changes ▁the ▁location ▁of ▁coh es in ▁indicating ▁that ▁local ization ▁of ▁coh es in ▁may ▁depend ▁on ▁trans cription . ▁ ▁In ▁another ▁model , ▁chrom atin ▁loop ▁extr usion ▁is ▁pushed ▁by ▁trans cription ▁generated ▁super co iling ▁ens uring ▁also ▁that ▁coh es in ▁re local izes