on ist ▁discovered ▁to ▁date ▁( EC 5 0 ▁= ▁ 5 0 ▁p M ). ▁The ▁p yr im id ine ▁P F - 4 8 4 0 1 5 4 ▁is ▁a ▁pot ent , ▁non - c oval ent ▁activ ator ▁of ▁both ▁the ▁human ▁( EC 5 0 ▁= ▁ 2 3 ▁n M ) ▁and ▁rat ▁( EC 5 0 ▁= ▁ 9 7 ▁n M ) ▁Tr p A 1 ▁channels . ▁This ▁comp ound ▁el ic its ▁n oc ice ption ▁in ▁a ▁mouse ▁model ▁through ▁Tr p A 1 ▁activation . ▁Furthermore , ▁P F - 4 8 4 0 1 5 4 ▁is ▁superior ▁to ▁al ly l ▁is oth i oc yan ate , ▁the ▁p ung ent ▁component ▁of ▁must ard ▁oil , ▁for ▁screen ing ▁purposes . ▁ ▁The ▁e icos ano ids ▁formed ▁in ▁the ▁A LO X 1 2 ▁( i . e . ▁a rach id on ate - 1 2 - li po xy g ne ase ) ▁path way ▁of ▁a rach id onic ▁acid ▁met abol ism , ▁ 1 2 S - h ydro per ox y - 5 Z , 8 Z , 1 0 E , 1 4 Z - e icos at etra eno ic ▁acid ▁( i . e . ▁ 1 2 S - H p ET E ; ▁see ▁ 1 2 - H ydro xy e icos at etra eno ic ▁acid ) ▁and ▁the ▁he po