f isk ▁of ▁several ▁try ptic ▁pe pt ides ▁of ▁standard ▁prote ins , ▁by ▁the ▁quad ru pole ▁time - of - fl ight ( Q - TO F ) ▁mass ▁spect rom eter . ▁ ▁P ep N ovo ▁is ▁a ▁high ▁through put ▁de ▁nov o ▁pe pt ide ▁sequ encing ▁tool ▁and ▁uses ▁a ▁probabil istic ▁network ▁as ▁scoring ▁method . ▁It ▁usually ▁takes ▁less ▁than ▁ 0 . 2 ▁seconds ▁for ▁interpretation ▁of ▁one ▁spectrum . ▁Descri bed ▁by ▁Frank ▁et ▁al , ▁P ep N ovo ▁works ▁better ▁than ▁several ▁popular ▁algorithms ▁like ▁Sher eng a , ▁P E A KS , ▁L ute f isk . ▁Now ▁a ▁new ▁version ▁P ep N ovo + ▁is ▁available . ▁ ▁Chi ▁et ▁al ▁presented ▁p N ovo + ▁in ▁ 2 0 1 3 ▁as ▁a ▁new ▁de ▁nov o ▁pe pt ide ▁sequ encing ▁tool ▁by ▁using ▁complement ary ▁H CD ▁and ▁E TD ▁tan dem ▁mass ▁spect ra . ▁In ▁this ▁method , ▁a ▁component ▁algorithm , ▁p DA G , ▁largely ▁spe eds ▁up ▁the ▁ac quisition ▁time ▁of ▁pe pt ide ▁sequ encing ▁to ▁ 0 . 0 1 8 s ▁on ▁average , ▁which ▁is ▁three ▁times ▁as ▁fast ▁as ▁the ▁other ▁popular ▁de ▁nov o ▁sequ encing ▁software . ▁ ▁As ▁described ▁by ▁Je ong ▁et ▁al , ▁compared ▁with ▁other ▁do ▁nov o ▁pe pt ide ▁sequ encing ▁tools , ▁which ▁works ▁well ▁on ▁only ▁certain ▁types ▁of ▁spect ra