▁of ▁a ▁hom o zy g ous , ▁hy pert ensive ▁trans gen ic ▁rat ▁model ▁of ▁ren in ▁over expression , ▁in ▁which ▁a ▁population ▁of ▁these ▁r ats ▁rapidly ▁develop ▁heart ▁failure ▁and ▁another ▁remains ▁compens ated . ▁Out ▁of ▁ 1 4 3 ▁differential ly - reg ulated ▁gen es , ▁LI MP - 2 ▁was ▁identified ▁to ▁be ▁significantly ▁up reg ulated ▁in ▁heart ▁failure - pr one ▁rat ▁card iac ▁mus cle ▁bi ops ies , ▁which ▁also ▁proved ▁true ▁in ▁human ▁heart ▁failure . ▁Further ▁analysis ▁employ ing ▁a ▁LI MP - 2 ▁knock out ▁mouse ▁demonstrated ▁that ▁animals ▁lack ing ▁LI MP - 2 ▁failed ▁to ▁flight ▁a ▁normal ▁hy pert roph ic ▁response ▁following ▁An gi ot ens in ▁II ▁treatment , ▁however ▁they ▁developed ▁inter st it ial ▁fib ros is ▁and ▁dil ated ▁card iom y op ath y ▁coordinate ▁with ▁dis rupted ▁inter cal ated ▁disc ▁structure . ▁Bio chem ical ▁and ▁imm un oh ist och em ical ▁anal ys es ▁discovered ▁that ▁LI MP - 2 ▁interact s ▁with ▁N - c ad her in ▁at ▁inter cal ated ▁disc s , ▁a ▁function ▁outside ▁of ▁l ys os om al ▁memb ran es . ▁Kno ck down ▁of ▁LI MP - 2 ▁with ▁R NA ▁inter ference ▁decre ased ▁the ▁binding ▁of ▁N - c ad her in ▁to ▁the ▁ph osph ory l ated ▁form ▁of ▁beta - c aten in , ▁and ▁LI MP - 2 ▁over