ite ▁out g row th ▁in hib itor ▁( N ogo ), ▁in hib its ▁cell ▁growth ▁through ▁the ▁activation ▁of ▁r ho ▁kin ase ▁( RO CK ). ▁ ▁Ng R ▁activation ▁of ▁p 7 5 ▁ ▁It ▁was ▁known ▁that ▁Ng R , ▁N ogo , ▁and ▁another ▁memb rane ▁re ceptor ▁called ▁p 7 5 ▁were ▁involved ▁in ▁in hib iting ▁neur ite ▁out g row th . ▁ ▁Through ▁a ▁variety ▁of ▁experimental ▁procedures ▁Wang ▁et ▁al . ▁were ▁able ▁to ▁identify ▁the ▁bio chem ical ▁relationship ▁between ▁Ng R ▁and ▁p 7 5 . ▁ ▁First , ▁it ▁was ▁observed ▁that ▁when ▁p 7 5 ▁was ▁knock ed ▁out ▁in ▁m ice , ▁out g row th ▁in hib ition ▁was ▁no ▁longer ▁seen . ▁ ▁Comple ting ▁binding ▁ass ays ▁and ▁co - imm un op reci pit ations ▁revealed ▁that ▁p 7 5 ▁and ▁Ng R ▁were ▁not ▁bound ▁to ▁each ▁other ▁through ▁the ▁cell ular ▁memb rane . ▁ ▁Mut ating ▁either ▁p 7 5 ▁or ▁Ng R , ▁however , ▁resulted ▁in ▁trunc ated ▁protein ▁that ▁would ▁help ▁reve al ▁the ▁binding ▁interactions . ▁ ▁When ▁the ▁ext rac ell ular ▁domains ▁of ▁the ▁re cept ors ▁were ▁removed ▁no ▁out g row th ▁in hib ition ▁was ▁seen . ▁ ▁This ▁would ▁suggest ▁that ▁the ▁re cept ors ▁interact ▁ext rac ell ular ly . ▁ ▁Furthermore , ▁it ▁was ▁re aff ir med ▁that ▁N ogo ▁and ▁my elin - associ ated