▁target ▁identification . ▁ ▁Re verse ▁chem ogen om ics ▁▁ ▁In ▁reverse ▁chem ogen om ics , ▁small ▁comp ounds ▁that ▁perturb ▁the ▁function ▁of ▁an ▁en zym e ▁in ▁the ▁context ▁of ▁an ▁in ▁vit ro ▁en zym atic ▁test ▁will ▁be ▁identified . ▁ ▁Once ▁the ▁mod ul ators ▁have ▁been ▁identified , ▁the ▁phen ot ype ▁induced ▁by ▁the ▁mole c ule ▁is ▁analyz ed ▁in ▁a ▁test ▁on ▁cells ▁or ▁on ▁whole ▁organ isms . ▁ ▁This ▁method ▁will ▁identify ▁or ▁confirm ▁the ▁role ▁of ▁the ▁en zym e ▁in ▁the ▁bi ological ▁response . ▁Re verse ▁chem ogen om ics ▁used ▁to ▁be ▁virt ually ▁identical ▁to ▁the ▁target - based ▁approaches ▁that ▁have ▁been ▁applied ▁in ▁drug ▁discovery ▁and ▁mole cular ▁ph arm ac ology ▁over ▁the ▁past ▁de cade . ▁ ▁This ▁strategy ▁is ▁now ▁en hanced ▁by ▁parallel ▁screen ing ▁and ▁by ▁the ▁ability ▁to ▁perform ▁lead ▁optimization ▁on ▁many ▁targets ▁that ▁belong ▁to ▁one ▁target ▁family . ▁ ▁App lications ▁ ▁Det erm ining ▁mode ▁of ▁action ▁ ▁Chem ogen om ics ▁has ▁been ▁used ▁to ▁identify ▁mode ▁of ▁action ▁( MO A ) ▁for ▁traditional ▁Chinese ▁medicine ▁( TC M ) ▁and ▁Ay ur ved a . ▁Comp ounds ▁contained ▁in ▁traditional ▁medic ines ▁are ▁usually ▁more ▁sol ub le ▁than ▁synth etic ▁comp ounds , ▁have ▁“ priv ile ged ▁structures ” ▁( chem ical ▁structures ▁that ▁are ▁more ▁frequently ▁found ▁to ▁bind ▁in ▁different ▁living ▁organ isms ), ▁and ▁have