▁maintain ▁the ▁intern als ▁of ▁an ▁object ▁without ▁changing ▁the ▁interface , ▁one ▁of ▁the ▁core ▁ten ets ▁of ▁Object ▁Ori ented ▁Analysis ▁and ▁Design . ▁ ▁See ▁also ▁Class - Res p ons ibility - Coll abor ation ▁card ▁Un ified ▁Model ing ▁Language ▁Object - orient ed ▁analysis ▁Object - orient ed ▁design ▁ ▁References ▁ ▁Category : Ant i - pattern s <0x0A> </s> ▁Ol ub o ▁is ▁an ▁eth nic ▁group ▁of ▁Equ ator ia ▁in ▁South ▁Sud an . ▁They ▁speak ▁O lu ' bo , ▁a ▁N ilo - S ah aran ▁language . ▁The ▁population ▁of ▁this ▁group ▁may ▁exceed ▁ 1 0 , 0 0 0 . ▁Most ▁persons ▁in ▁this ▁minor ity ▁are ▁not ▁Muslim s , ▁although ▁some ▁are . ▁ ▁References ▁▁▁ ▁Category : E th nic ▁groups ▁in ▁South ▁Sud an <0x0A> </s> ▁GC a MP ▁is ▁a ▁gen et ically ▁encoded ▁calci um ▁indicator , ▁or ▁G E CI ▁initially ▁developed ▁by ▁Jun ichi ▁Nak ai . ▁GC a MP ▁is ▁created ▁from ▁a ▁fusion ▁of ▁ ▁green ▁flu ores cent ▁protein ▁( G FP ), ▁cal mod ul in , ▁and ▁M 1 3 , ▁a ▁pe pt ide ▁sequence ▁from ▁my os in ▁light ▁chain ▁kin ase . ▁ ▁The ▁advantage ▁of ▁G E CI ' s ▁is ▁that ▁they ▁can ▁be ▁gen et ically ▁specified ▁for ▁studies ▁in ▁living ▁organ isms . ▁ ▁The ▁first ▁trans gen ic ▁mouse ▁express ing ▁a ▁GC a MP ▁was ▁reported