▁and , ▁in ▁ 1 7 1 8 , ▁had ▁her ▁son ▁successfully ▁vari ol ated ▁in ▁Constantin ople ▁under ▁the ▁super vision ▁of ▁Dr . ▁Charles ▁M ait land . ▁When ▁she ▁returned ▁to ▁England ▁in ▁ 1 7 2 1 , ▁she ▁had ▁her ▁daughter ▁vari ol ated ▁by ▁M ait land . ▁This ▁ar oused ▁considerable ▁interest , ▁and ▁Sir ▁Hans ▁Slo ane ▁organized ▁the ▁vari olation ▁of ▁some ▁in m ates ▁in ▁New gate ▁Pr ison . ▁These ▁were ▁successful , ▁and ▁after ▁a ▁further ▁short ▁trial ▁in ▁ 1 7 2 2 , ▁two ▁daughters ▁of ▁Caroline ▁of ▁Ans bach ▁Princess ▁of ▁Wales ▁were ▁vari ol ated ▁without ▁m ish ap . ▁With ▁this ▁royal ▁appro val , ▁the ▁procedure ▁became ▁common ▁when ▁small po x ▁ep ide m ics ▁threatened . ▁ ▁Relig ious ▁arguments ▁against ▁in oc ulation ▁were ▁soon ▁advanced . ▁For ▁example , ▁in ▁a ▁ 1 7 7 2 ▁ser mon ▁entitled ▁" The ▁D anger ous ▁and ▁Sin ful ▁Pract ice ▁of ▁In oc ulation ", ▁the ▁English ▁the olog ian ▁Re ver end ▁Edmund ▁M asse y ▁argued ▁that ▁dise ases ▁are ▁sent ▁by ▁God ▁to ▁pun ish ▁sin ▁and ▁that ▁any ▁attempt ▁to ▁prevent ▁small po x ▁via ▁in oc ulation ▁is ▁a ▁" di abol ical ▁operation ". ▁It ▁was ▁custom ary ▁at ▁the ▁time ▁for ▁popular ▁pre ach ers ▁to ▁publish ▁ser m ons , ▁which ▁reached ▁a ▁wide ▁audience . ▁This ▁was ▁the ▁case ▁with ▁M asse