▁same ▁scoring ▁outcome , ▁but ▁use ▁differ ing ▁method ologies . ▁B L OS UM ▁directly ▁look ▁at ▁mut ations ▁in ▁mot ifs ▁of ▁related ▁sequences ▁while ▁P AM ' s ▁ext rap olate ▁evolution ary ▁information ▁based ▁on ▁closely ▁related ▁sequences . ▁ ▁Since ▁both ▁P AM ▁and ▁B L OS UM ▁are ▁different ▁methods ▁for ▁showing ▁the ▁same ▁scoring ▁information , ▁the ▁two ▁can ▁be ▁compared ▁but ▁due ▁to ▁the ▁very ▁different ▁method ▁of ▁obtain ing ▁this ▁score , ▁a ▁P AM 1 0 0 ▁does ▁not ▁equal ▁a ▁B L OS UM 1 0 0 . ▁ ▁See ▁also ▁ ▁Point ▁mut ation ▁ ▁Se quence ▁alignment ▁ ▁Margaret ▁Day hoff ▁ ▁M ole cular ▁clock ▁ ▁B L OS UM ▁ ▁B LA ST ▁ ▁References ▁ ▁External ▁links ▁ ▁http :// www . inf . eth z . ch / person al / gon net / D ar win Man ual / node 1 4 8 . html ▁ ▁http :// www . bio in format ics . nl / tools / p am . html ▁For ▁quickly ▁calculating ▁a ▁P AM ▁matrix . ▁ ▁http :// web . exp asy . org / docs / rel notes / rel stat . html ▁The ▁most ▁recent ▁statistics ▁from ▁the ▁Swiss - Pro t ▁protein ▁knowledge base . ▁Section ▁ 6 . 1 ▁contains ▁the ▁most ▁up - to - date ▁am ino ▁acid ▁frequencies ▁ ▁Category : M ut ation ▁Category : B io in format ics <0x0A>