▁is ▁couple d ▁together ▁before ▁further ▁operations ▁are ▁performed ▁to ▁finally ▁form ▁con i ine . ▁▁ ▁Initial ly , ▁ac et ate ▁is ▁converted ▁to ▁ac et yl - Co A , ▁some ▁of ▁which ▁is ▁also ▁used ▁to ▁form ▁mal ony l ▁Co A . ▁An ▁ac et yl ▁Co A ▁is ▁further ▁el ong ated ▁using ▁mal ony l - Co A ▁by ▁fat ty ▁acid ▁synth ase ▁to ▁form ▁but y ry l - Co A . ▁▁ ▁Further ▁el ong ation ▁of ▁but y ry l - Co A ▁using ▁ 2 ▁mal ony l - Co A ▁forms ▁ 5 - ket oo ct anal . ▁ ▁K et oo ct anal ▁then ▁under go es ▁trans am ination ▁using ▁ ▁al an ine : 5 - ket o - oct anal ▁am in otr ans fer ase . ▁The ▁am ine ▁then ▁sp ont ane ously ▁cycl izes ▁and ▁is ▁de h yd r ated ▁to ▁form ▁the ▁con i ine ▁prec ursor ▁ γ – con ice ine . ▁ ▁This ▁is ▁then ▁reduced ▁using ▁N AD PH ▁dependent ▁y - con ice ine ▁re duct ase ▁to ▁form ▁con i ine . ▁ ▁In ▁popular ▁culture ▁Con i ine ▁is ▁the ▁murder ▁weapon ▁in ▁Ag ath a ▁Christ ie ' s ▁mystery ▁novel ▁Five ▁Little ▁P igs . ▁ ▁References ▁ ▁Further ▁reading ▁ ▁External ▁links ▁▁ ▁Information ▁on ▁hem lock ▁from ▁the ▁University ▁of ▁Br istol ▁▁ ▁Mitch ▁T ucker ▁student ▁work ,