acter ial ▁gen ome , ▁the ▁pl as mid ▁stands ▁a ▁high ▁chance ▁of ▁not ▁being ▁passed ▁to ▁both ▁daughter ▁cells . ▁The ▁P 1 ▁pl as mid ▁comb ats ▁this ▁by ▁several ▁methods : ▁The ▁pl as mid ▁rep lication ▁is ▁tight ly ▁reg ulated ▁by ▁a ▁Rep A ▁protein ▁dependent ▁mechanism . ▁This ▁is ▁similar ▁to ▁the ▁mechanism ▁used ▁by ▁several ▁other ▁pl asm ids . ▁It ▁ensure ▁that ▁the ▁pl as mid ▁div ides ▁in ▁step ▁with ▁the ▁host ▁gen ome . ▁Inter locked ▁pl asm ids ▁are ▁quickly ▁un link ed ▁by ▁Cre - lo x ▁recom bin ation ▁The ▁pl as mid ▁enc odes ▁a ▁pl as mid ▁add iction ▁system ▁that ▁k ills ▁daughter ▁cells ▁that ▁lose ▁the ▁pl as mid . ▁It ▁consists ▁of ▁a ▁stable ▁protein ▁to x in ▁and ▁an ▁ant ito x in ▁that ▁revers ibly ▁bind s ▁to ▁and ▁neutral izes ▁it . ▁C ells ▁that ▁lose ▁the ▁pl as mid ▁get ▁killed ▁as ▁the ▁ant ito x in ▁gets ▁de grad ed ▁faster ▁than ▁the ▁to x in . ▁ ▁L ysis ▁The ▁P 1 ▁pl as mid ▁has ▁a ▁separate ▁origin ▁of ▁rep lication ▁( ori L ) ▁that ▁is ▁activ ated ▁during ▁the ▁l yt ic ▁cycle . ▁Rep lication ▁begins ▁by ▁a ▁regular ▁bid irection al ▁the ta ▁rep lication ▁at ▁or i L ▁but ▁later ▁in ▁the ▁l yt ic ▁phase , ▁it ▁switch es ▁to ▁a ▁rolling ▁circle ▁method ▁of ▁rep lication ▁using ▁the ▁host ▁recom