rol ase ▁() ▁is ▁an ▁en zym e ▁in ▁the ▁family ▁of ▁hyd rol ases ▁that ▁specifically ▁acts ▁on ▁th io ester ▁b onds . ▁It ▁catal y zes ▁the ▁hydro ly s is ▁of ▁long ▁chain ▁fat ty ▁a cy l ▁th io esters ▁of ▁a cy l ▁car rier ▁protein ▁or ▁co enz yme ▁A ▁to ▁form ▁free ▁fat ty ▁acid ▁and ▁the ▁respective ▁th i ol . ▁ ▁Thus , ▁the ▁two ▁substr ates ▁of ▁this ▁en zym e ▁are ▁pal m ito yl - Co A ▁and ▁H 2 O , ▁whereas ▁its ▁two ▁products ▁are ▁Co A ▁and ▁pal mit ate . ▁It ▁has ▁a ▁strict ▁specific ity ▁for ▁th io esters ▁with ▁a ▁chain ▁link ▁greater ▁than ▁C 1 0 . ▁ ▁These ▁en zym es ▁are ▁local ized ▁in ▁almost ▁all ▁cell ular ▁compart ments , ▁such ▁as ▁end op las mic ▁ret icul um , ▁c yt os ol , ▁mit och ond ria , ▁and ▁per ox is om es . ▁They ▁are ▁highly ▁reg ulated ▁by ▁per ox is ome ▁pro l ifer ator ▁activ ated ▁re cept ors , ▁which ▁led ▁to ▁their ▁invol vement ▁in ▁li pid ▁met abol ism . ▁The ▁en zym e ▁is ▁up - reg ulated ▁during ▁times ▁of ▁increased ▁fat ty ▁acid ▁ox id ation , ▁which ▁suggests ▁that ▁this ▁en zym e ▁has ▁a ▁potential ▁role ▁the ▁per ox is om al ▁beta - ox id ation . ▁ ▁The ▁system atic ▁name ▁of ▁this