▁has ▁ten ▁sites ▁of ▁potential ▁g ly cos yl ation , ▁and ▁many ▁of ▁these ▁sites ▁( including ▁some ▁far ▁from ▁the ▁catal yt ic ▁domain ) ▁affect ▁the ▁ability ▁of ▁GC PI I ▁to ▁hydro ly ze ▁N AA G . ▁ ▁The ▁F OL H 1 ▁gene ▁has ▁multiple ▁potential ▁start ▁sites ▁and ▁sp lice ▁forms , ▁giving ▁rise ▁to ▁differences ▁in ▁memb rane ▁protein ▁structure , ▁local ization , ▁and ▁car box ype pt id ase ▁activity ▁based ▁on ▁the ▁parent ▁t issue . ▁ ▁En zym e ▁k inet ics ▁ ▁The ▁hydro ly s is ▁of ▁N AA G ▁by ▁GC PI I ▁ob e ys ▁Michael is - M enten ▁k inet ics ▁calculated ▁the ▁binding ▁constant ▁( K m ) ▁for ▁N AA G ▁as ▁approximately ▁ 1 3 0 ▁n M ▁and ▁the ▁turn over ▁constant ▁( k cat ) ▁as ▁approximately ▁ 4 ▁s − 1 . ▁The ▁apparent ▁second - order ▁rate ▁constant ▁is ▁approximately ▁ 3 ▁x ▁ 1 0 7 ▁( M · s ) − 1 . ▁ ▁Ro le ▁in ▁cancer ▁ ▁Human ▁PS MA ▁is ▁highly ▁expressed ▁in ▁the ▁pro state , ▁roughly ▁a ▁hundred ▁times ▁greater ▁than ▁in ▁most ▁other ▁t issues . ▁In ▁some ▁pro state ▁can cers , ▁PS MA ▁is ▁the ▁second - most ▁up reg ulated ▁gene ▁product , ▁with ▁an ▁ 8 - ▁to ▁ 1 2 - fold ▁increase ▁over ▁levels ▁in ▁non can cer ous ▁pro state ▁cells . ▁