▁The ▁recom bin ant ▁en zym e ▁from ▁black ▁pe pp erm int ▁produces ▁a ▁mixture ▁of ▁c is - mu uro la - 3 , 5 - d iene ▁and ▁c is - mu uro la - 4 ( 1 4 ), 5 - d iene . ▁ ▁References ▁ ▁External ▁links ▁▁▁▁ ▁Category : EC ▁ 4 . 2 . 3 <0x0A> </s> ▁P wo ▁is ▁a ▁sacred ▁init iation ▁rit ual , ▁in ▁which ▁students ▁of ▁traditional ▁navigation ▁in ▁the ▁Caroline ▁Islands ▁in ▁Mic ron esia ▁become ▁navig ators ▁( pal u ) ▁and ▁are ▁initi ated ▁in ▁the ▁associated ▁secre ts . ▁Many ▁island ers ▁in ▁the ▁area ▁indicate ▁that ▁this ▁ceremony ▁origin ated ▁on ▁the ▁island ▁of ▁Pol lap , ▁or ▁nearby ▁islands . ▁ ▁The ▁P wo ▁ceremony ▁today ▁is ▁having ▁a ▁com eb ack ▁in ▁importance . ▁ ▁In ▁the ▁days ▁before ▁World ▁War ▁II , ▁it ▁was ▁common ▁to ▁have ▁P wo ▁take ▁place . ▁ ▁After ▁W W II , ▁through ▁western ization ▁and ▁the ▁influence ▁of ▁mission aries ▁traditional ▁practices ▁including ▁P wo ▁started ▁to ▁be ▁abandoned . ▁ ▁Mau ▁Pia il ug , ▁famous ▁for ▁helping ▁Hawai ians ▁reg ain ▁their ▁traditional ▁navigation ▁skills , ▁was ▁the ▁last ▁person ▁to ▁go ▁through ▁the ▁P wo ▁ceremony ▁in ▁ 1 9 5 1 . ▁ ▁For ▁thirty - n ine ▁years , ▁the ▁ceremony ▁lay ▁d orm ant . ▁ ▁Then , ▁in ▁May ▁ 1 9 9 0 , ▁P wo ▁again