▁CO 2 ▁atmosphere ▁or ▁in ▁ambient ▁air . ▁Is ol ates ▁were ▁then ▁grown ▁at ▁seven ▁different ▁temper atures ▁in ▁an ▁an a er ob ic ▁environment , ▁reaching ▁optim um ▁growth ▁at ▁ 3 0 ▁to ▁ 3 4   ° C . ▁Cult ures ▁were ▁dil uted ▁in ▁bro th ▁medium ▁then ▁passed ▁through ▁memb rane ▁filters ▁of ▁various ▁p ore ▁diam eters , ▁yield ing ▁similar ▁results ▁for ▁all ▁nine ▁isol ates . ▁D il uted ▁isol ates ▁were ▁then ▁tested ▁for ▁re version , ▁a ▁method ▁of ▁gen etic ▁stabil ization ▁unique ▁to ▁some ▁b acter ia , ▁in ▁both ▁bro th ▁and ▁ag ar ▁medium . ▁No ▁re version ▁was ▁observed . ▁Is ol ates ▁were ▁grown ▁in ▁bro th ▁medium ▁with ▁large ▁( 2 0 %) ▁and ▁minute ▁( 0 . 2 %) ▁amounts ▁of ▁f etal ▁b ov ine ▁ser um ▁as ▁a ▁ster ol ▁source . ▁G row th ▁was ▁in hib ited ▁in ▁the ▁latter , ▁indicating ▁dependence ▁on ▁an ▁outside ▁ster ol ▁source ▁for ▁growth . ▁The ▁ 1 6 S ▁r R NA ▁gene ▁was ▁sequ enced , ▁yield ing ▁a ▁unique ▁sequence , ▁suggesting ▁the ▁discovery ▁of ▁a ▁previously ▁un ident ified ▁organ ism . ▁The ▁Brown ▁study ▁in ▁ 2 0 0 1 ▁further ▁investig ated ▁the ▁path ogen icity ▁of ▁the ▁newly ▁discovered ▁M . ▁all ig ator is ▁by ▁in oc ulation ▁of ▁four ▁health y ▁all ig ators ▁with ▁the ▁b acter ia ▁and ▁one ▁control ▁all