Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex
Ligang Wang, Hongkuan Yang, Shiguang Zhao, Haruhisa Sato, Yoshihiro Konishi, Thomas G. Beach, Essam Mohamed, Naomi J. Bisem, Ikuo Tooyama
Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H 2O 2 was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.
Ferritins
neuroprotective effect
mitochondrial messenger RNA
Real-Time Polymerase Chain Reaction
Wang, L., Yang, H., Zhao, S., Sato, H., Konishi, Y., Beach, T. G., ... Tooyama, I. (2011). Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex. PLoS One, 6(7), [e22325]. https://doi.org/10.1371/journal.pone.0022325
Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex. / Wang, Ligang; Yang, Hongkuan; Zhao, Shiguang; Sato, Haruhisa; Konishi, Yoshihiro; Beach, Thomas G.; Mohamed, Essam; Bisem, Naomi J.; Tooyama, Ikuo.
In: PLoS One, Vol. 6, No. 7, e22325, 26.07.2011.
Wang, L, Yang, H, Zhao, S, Sato, H, Konishi, Y, Beach, TG, Mohamed, E, Bisem, NJ & Tooyama, I 2011, 'Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex', PLoS One, vol. 6, no. 7, e22325. https://doi.org/10.1371/journal.pone.0022325
Wang L, Yang H, Zhao S, Sato H, Konishi Y, Beach TG et al. Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex. PLoS One. 2011 Jul 26;6(7). e22325. https://doi.org/10.1371/journal.pone.0022325
Wang, Ligang ; Yang, Hongkuan ; Zhao, Shiguang ; Sato, Haruhisa ; Konishi, Yoshihiro ; Beach, Thomas G. ; Mohamed, Essam ; Bisem, Naomi J. ; Tooyama, Ikuo. / Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex. In: PLoS One. 2011 ; Vol. 6, No. 7.
@article{70cac4357bb44e829211faa5fab9bcee,
title = "Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex",
abstract = "Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H 2O 2 was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.",
author = "Ligang Wang and Hongkuan Yang and Shiguang Zhao and Haruhisa Sato and Yoshihiro Konishi and Beach, {Thomas G.} and Essam Mohamed and Bisem, {Naomi J.} and Ikuo Tooyama",
T1 - Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex
AU - Wang, Ligang
AU - Yang, Hongkuan
AU - Zhao, Shiguang
AU - Sato, Haruhisa
AU - Konishi, Yoshihiro
AU - Beach, Thomas G.
AU - Mohamed, Essam
AU - Bisem, Naomi J.
AU - Tooyama, Ikuo
N2 - Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H 2O 2 was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.
AB - Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H 2O 2 was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.