CrkL pY207 Antibody, anti-human, REAfinity™
CrkL pY207 Antibody, anti-human, REAfinity™Recombinant
Crk-like
Intracellular flow cytometry applications forCrkL pY207 Antibody, anti-human, REAfinity™
Intracellular flow cytometry staining protocol (Cell Signaling Buffer Set A 1:11)
Human K-562 cells were either left untreated (right peak) or treated with 25 µM Imatinib for 2 hours at 37 °C. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-CrkL pY207 antibodies. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Specifications for CrkL pY207 Antibody, anti-human, REAfinity™
Clone REA500 recognizes the human CrkL antigen phosphorylated at tyrosine 207 (pY207). CRKL is a member of adaptor proteins that participate in signal transduction in response to both extracellular and intracellular stimuli, such as growth factors, cytokines, and the oncogenic BCR-ABL fusion protein. CRKL consists of an N-terminal Src homology 2 (SH2) domain followed by two SH3 domains (SH2-SH3N-SH3C). The SH2 domain of CRKL binds to phosphorylated Y-x-x-P motif present in many docking proteins, such as BCAR1, Paxillin, and GAB, whereas the SH3N domain binds to proline-rich P-x-x-P-x-K motif-containing proteins, such as Son of Sevenless (SOS), RAPGEF1, p85, ABL1, and BCR-ABL. Through these interactions, CRKL facilitates the timely and localized formation of protein complexes required for signal transduction in many biological processes including cell proliferation, survival, adhesion, and migration. CRKL is preferentially expressed in hematopoietic cells. Y207 in CRKL represents the major
phosphorylation site. It is phosphorylated in hematopoietic cells that express the BCR-ABL fusion protein. Phosphorylation of Y207 provides a binding site for the CRKL SH2 domain and potentially for other SH2-containing proteins.
CrkL pY207
Resources for CrkL pY207 Antibody, anti-human, REAfinity™
References for CrkL pY207 Antibody, anti-human, REAfinity™
ten Hoeve, J. et al. (2002) Identification of a nuclear Stat1 protein tyrosine phosphatase. Mol. Cell. Biol. 16: 5662-5668
de Jong, R. et al. (1997) Tyrosine 207 in CRKL is the BCR/ABL phosphorylation site. Oncogene 14(5): 507-513
Park, T. J. et al. (2014) Essential roles of Crk and CrkL in fibroblast structure and motility. Oncogene 33(43): 5121-5132
Cell Signaling Buffer Set A
The Cell Signaling Buffer Set A has been developed for use with MACS® Cell Signaling Antibodies. It ...