The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
Makoto Hayashida, Yuko Nishimoto, Toshiharu Matsuura, Yukiko Takahashi, Kenichi Kohashi, Ryota Souzaki, Tomoaki Taguchi
Reproductive and Developmental Medicine
Background: Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA. Methods: The liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin. Results: The frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non-BA group were 3.00 ± 0.75 and 0.99 ± 0.50, respectively (P = .005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells. Conclusion: The presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.
Journal of Pediatric Surgery
https://doi.org/10.1016/j.jpedsurg.2007.08.039
10.1016/j.jpedsurg.2007.08.039
Fingerprint Dive into the research topics of 'The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization'. Together they form a unique fingerprint.
Biliary Atresia Medicine & Life Sciences
Chimerism Medicine & Life Sciences
Fluorescence In Situ Hybridization Medicine & Life Sciences
Mothers Medicine & Life Sciences
Y Chromosome Medicine & Life Sciences
X Chromosome Medicine & Life Sciences
Graft vs Host Disease Medicine & Life Sciences
Hayashida, M., Nishimoto, Y., Matsuura, T., Takahashi, Y., Kohashi, K., Souzaki, R., & Taguchi, T. (2007). The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization. Journal of Pediatric Surgery, 42(12), 2097-2101. https://doi.org/10.1016/j.jpedsurg.2007.08.039
The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization. / Hayashida, Makoto; Nishimoto, Yuko; Matsuura, Toshiharu; Takahashi, Yukiko; Kohashi, Kenichi; Souzaki, Ryota; Taguchi, Tomoaki.
In: Journal of Pediatric Surgery, Vol. 42, No. 12, 12.2007, p. 2097-2101.
Hayashida, M, Nishimoto, Y, Matsuura, T, Takahashi, Y, Kohashi, K, Souzaki, R & Taguchi, T 2007, 'The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization', Journal of Pediatric Surgery, vol. 42, no. 12, pp. 2097-2101. https://doi.org/10.1016/j.jpedsurg.2007.08.039
Hayashida M, Nishimoto Y, Matsuura T, Takahashi Y, Kohashi K, Souzaki R et al. The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization. Journal of Pediatric Surgery. 2007 Dec;42(12):2097-2101. https://doi.org/10.1016/j.jpedsurg.2007.08.039
Hayashida, Makoto ; Nishimoto, Yuko ; Matsuura, Toshiharu ; Takahashi, Yukiko ; Kohashi, Kenichi ; Souzaki, Ryota ; Taguchi, Tomoaki. / The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization. In: Journal of Pediatric Surgery. 2007 ; Vol. 42, No. 12. pp. 2097-2101.
@article{48fb3c8b87a441a1a0838a27238dcd4b,
title = "The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization",
abstract = "Background: Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA. Methods: The liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin. Results: The frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non-BA group were 3.00 ± 0.75 and 0.99 ± 0.50, respectively (P = .005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells. Conclusion: The presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.",
author = "Makoto Hayashida and Yuko Nishimoto and Toshiharu Matsuura and Yukiko Takahashi and Kenichi Kohashi and Ryota Souzaki and Tomoaki Taguchi",
doi = "10.1016/j.jpedsurg.2007.08.039",
journal = "Journal of Pediatric Surgery",
publisher = "W.B. Saunders Ltd",
T1 - The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
AU - Hayashida, Makoto
AU - Nishimoto, Yuko
AU - Matsuura, Toshiharu
AU - Takahashi, Yukiko
AU - Kohashi, Kenichi
AU - Souzaki, Ryota
AU - Taguchi, Tomoaki
N2 - Background: Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA. Methods: The liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin. Results: The frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non-BA group were 3.00 ± 0.75 and 0.99 ± 0.50, respectively (P = .005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells. Conclusion: The presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.
AB - Background: Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that graft-vs-host disease caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of graft-vs-host disease. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it plays a role in the etiology of BA. Methods: The liver biopsy specimens of 6 male patients with BA (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization. The cells with 2 sex chromosomes in the nuclei were counted. Cells with 1 X- and 1 Y-chromosomes were considered to be host cells, and those with 2 X-chromosome were considered to be of maternal origin. Results: The frequency of cells with XX chromosomes per 1000 host cells in the BA group and the non-BA group were 3.00 ± 0.75 and 0.99 ± 0.50, respectively (P = .005). Moreover, the age at the time of biopsy did not affect the number of chimeric cells. Conclusion: The presence of female cells in the liver of male patients with BA was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.
U2 - 10.1016/j.jpedsurg.2007.08.039
DO - 10.1016/j.jpedsurg.2007.08.039
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery