Wawan Setiawan
Graduate Study Program of Phytopathology, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Babakan Raya 3, Dramaga, Bogor, West Java 16680
Suryo Wiyono
Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java 16680
Efi Toding Tondok
Atit Kanti
Research Center for Biology, Indonesian Institute of Sciences Cibinong Jln. Raya Jakarta-Bogor KM 46, Cibinong, West Java
I Made Sudiana
Home > Vol 24, No 1 (2020) >	Setiawan
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani
https://doi.org/10.22146/jpti.43344
Wawan Setiawan(1), Suryo Wiyono(2*), Efi Toding Tondok(3), Atit Kanti(4), I Made Sudiana(5)
(1)&nbspGraduate Study Program of Phytopathology, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Babakan Raya 3, Dramaga, Bogor, West Java 16680
(2)&nbspDepartment of Plant Protection, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java 16680
(4)&nbspResearch Center for Biology, Indonesian Institute of Sciences Cibinong Jln. Raya Jakarta-Bogor KM 46, Cibinong, West Java
(*) Corresponding Author
Rhodotorula sp. is widely known as a biocontrol agent and is reported effective in controlling several diseases on crops. Rhodotorula minuta Dmg 16 BEP is an antagonist yeast from Indonesia which was effective in controlling various plant diseases. The objective of this research was to study the in vitro mode of action of R. minuta Dmg 16 BEP against Alternaria solani. The antibiosis ability of R. minuta Dmg 16 BEP to A. solani might be based on the activity of volatile compounds and not from its metabolites products. The growth of A. solani in paper disk assay and dual culture tests were not inhibited nor inhibitory zones developed, whereas the inverse petridish test showed the growth inhibition of A. solani. The results of the chitinolytic activity test on chitin agar and proteolytic on skim milk agar showed that no clear zone was formed. R. minuta has a strong hyperparasitic ability according to the slide culture test as the yeast cells surrounded A. solani hyphae and caused damage to some parts of hyphae.
Alternaria solani; antibiosis; hyperparasitism; R. minuta
Andrews, J.H., F. Harris, N. Spear, & E.V. Nordheim. 1993. Morphogenesis and Adhesion of Aureobasidium pullulans. Canadian Journal of Microbiology 40: 6–17.
Buzzini, P., C. Gasparetti, B. Turchetti, M. Cramarossa, A. Vaughan-Martini, A. Martini, U.M. Pagnoni, & L. Feroti. 2005. Production of Volatile Organic Compounds (VOC) by Yeasts Isolated from the Ascocarps of Black (Tuber melanosporum Vitt.) and White (Tuber magnatum Pico.) Truffles. Archives of Microbiology 184: 187–193.
Castoria, R., F. De Curtis, G. Lima, & V. De Cicco. 1997. Beta-(1,3)- glucanase Activity of Two Saprophytic Yeast and Possible Mode of Action as Biocontrol Agents against Postharvest Diseases. Postharvest Biology and Technology 12: 293–300.
Chan, Z., & S. Tian. 2005. Interaction of Antagonistic Yeasts against Postharvest Pathogens of Apple Fruit and Possible Mode of Action. Postharvest Biology and Technolology 36: 215–223.
Chen, P.H.,& J.Y. Chou. 2017. Screening and Identification of Yeasts Antagonistic to Pathogenic Fungi Show a Narrow Optimal pH Range for Antagonistic Activity. Polish Journal of Microbiology 66: 101-106.
De Beer, E.J., & M.B. Sherwood. 1945. The Paper-Disc Agar-Plate Method For The Assay Of Antibiotic Substances. Journal of Bacteriology 50: 459-467.
Di Francesco, A., L. Ugolini, L. Lazzeri, & M. Mari. 2015. Production of Volatile Organic Compounds by Aureobasidium pullulans as a Potential Mechanism of Action against Postharvest Fruit Pathogens. Biological Control 81: 58-64.
El-Ghaouth, A., C.L. Wilson, & M. Wisniewski. 2003. Control of Postharvest Decay of Apple Fruit with Candida saitoana and Induction of Defense Responses. Phytopathology 93: 344–348.
Gholamnejad, J., H.R. Etebarian & N. Sahebani. 2010. Biological Control of Apple Blue Mold with Candida membranifaciens and Rhodotorula mucilaginosa. African Journal of Food Science 4: 1-7.
Hartati, S., S. Wiyono, S.H. Hidayat, & M.S. Sinaga. 2014. Seleksi Khamir Epifit sebagai Agens Antagonis Penyakit Antraknosa pada Cabai. Jurnal Hortikultura 24: 258-265.
Hartati, S., S. Wiyono, S.H. Hidayat, & M.S. Sinaga. 2015. Mode of Action of Yeast-like Fungus Aureobasidium pullulans in Controlling Anthracnose of Postharvest Chili. International Journal of Innovative and Applied Research 4531: 253–263.
Jones, B.V., F. Sun & J.R. Marchesi. 2007. Using Skimmed Milk Agar To Functionally Screen A Gutmetagenomic Library For Proteases May Lead To False Positives. Letters in Applied Microbiology 45: 418–420
Kalay, A.M. & J.P.M. Sinay. 2015. Perkembangan Alternaria solani pada Tiga Varietas Tanaman Tomat. Jurnal Agrikultur 26: 1–6.
Li, R., H. Zhang, W. Liu, & X. Zheng. 2011. Biocontrol of Postharvest Gray and Blue Mold Decay of Apples with Rhodotorula mucilaginosa and Possible Mechanisms of Action. International Journal of Food Microbiology 146: 151–156.
Li, B., H. Peng, & S. Tian. 2016. Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy. Frontiers Microbiology 7: 601-609.
Liu, G.L., Z. Chi, G.Y. Wang, Z.P. Wang, Y. Li, & Z.M. Chi. 2013. Yeast Killer Toxins, Molecular Mechanisms of their Action and their Applications. Critical Reviews in Biotechnology.1-13.
Murthy, N., & B. Bleakley. 2012. Simplified Method of Preparing Colloidal Chitin Used for Screening of Chitinase- Producing Microorganisms. The Internet Journal of Microbiology 10: 1-5.
Nasreen N.E., M.B. Meah, F.H. Tumpa & M.A. Hossai. 2017. Effect of Media Composition on Vegetative and Reproductive Growth of Alternaria brassicicola and Bipolaris sorokiniana. Current Agriculture Research Journal 5 : 266-278.
Raharjo, K., C. Sumardiyono, N. Poesposendjojo, & Sismindari. 2006. Eksplorasi, Pengujian, dan Identifikasi Khamir Antagonis terhadap Patogen Antraknosa (Colletotrichum lagenarium) pada Semangka. Jurnal Perlindungan Tanaman Indonesia 12: 33-43.
Shahin, E., A. & J.F. Shepard. 1979. An Efficient Technique for Inducing Profuse Sporulation of Alternaria species. Phytopathology 69: 618-620.
Sjamsuridzal, W., A. Oetari, A. Kanti, R. Saraswati, C. Nakashima, Y Widyastuti, & A Katsuhiko. 2010. Ecological and Taxonomical Perspective of Yeast in Indonesia. Microbiology Indonesia 4: 60-68.
Spotts, R.A. & L.A. Cervantes. 2002. Involvement of Aureobasidium pullulans and Rhodotorula glutinis in Russet of D'anjou Pear Fruit. Plant Disease 86: 625-628.
Wabster, J., & R.W.S. Waber. 2007. Introduction to Fungi. Cambridge University Press, New York (USA). 841 p.
Wijedasa, M.H., & L V C Liyanapathirana. 2012. Evaluation of an Alternative Slide Culture Technique for the Morphological Identification of Fungal Spesies. Sri Lanka Journal of Infectious Diseases 2: 47-52.
Wisniewski, M., C. Biles, S. Droby, R. McLaughlin, C. Wilson, & E. Chalutz. 1991. Mode of Action of the Postharvest Biocontrol Yeast Pichia guilliermondii: Characterization of Attachment to Botrytis cinerea. Physiological and Molecular Plant Pathology 39: 245–258.
Zhang, H., Q. Yang, L. Ge , G. Zhang, X. Zhang, & X. Zhang. 2016. Chitin Enhances Biocontrol of Rhodotorula mucilaginosa to Postharvest Decay of Peaches. International Journal of Biological Macromolecules 88: 465-475.
DOI: https://doi.org/10.22146/jpti.43344
Abstract views : 224 | views : 114