evidence: Coronavirus Download PDFInfo - Publication number - US10130701B2 US10130701B2 US15/328,179 US201515328179A US10130701B2 US 10130701 B2 US10130701 B2 US 10130701B2 US 201515328179 A US201515328179 A US 201515328179A US 10130701 B2 US10130701 B2 US 10130701B2 - Authority - US - United States - Prior art keywords - coronavirus - seq - virus - replicase gene - protein - Prior art date - Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) [...] The coronavirus according to the first aspect of the invention has reduced pathogenicity compared to a coronavirus expressing a corresponding wild-type replicase, such that when the virus is administered to an embryonated egg, it is capable of replicating without being pathogenic to the embryo. [...] The coronavirus of the present invention may comprise a variant replicase gene which encodes a protein which comprises a mutation compared to any one of SEQ ID NO: 6, 7, 8 or 9 which, when expressed in a coronavirus, causes the virus to have reduced pathogenicity compared to a coronavirus expressing the corresponding wild-type replicase.