Provide a comprehensive explanation of the basic concepts and practical applications underlying fluorescence resonance energy transfer (FRET) in the context of laboratory research. Make sure to include a discussion of the relevant principles, such as how FRET works, the types of probes used, and the factors that can influence FRET efficiency. Also, describe the different applications of FRET in the laboratory setting, including the measurement of protein-protein interactions, the detection of enzymatic activity, and the monitoring of intracellular signaling pathways. Additionally, discuss the advantages and limitations of FRET as a research tool, and provide examples of recent advances in FRET-based techniques.
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