Biology
AgriculturalAndFoodSciences,1970-2015-Agricultural And Food Sciences-2011.jsonl.gz.gz
Agricultural And Food Sciences
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Root Distribution and Tiller Densities of Creeping Bentgrass Cultivars and Greens-type Annual Bluegrass Cultivars in a Putting Green

. Little knowledge exists regarding root distribution of creeping bentgrass ( Agrostis stolonifera ) and annual bluegrass ( Poa annua ) in root zones of golf course putting greens. To compare root distribution between these species, three experimental cultivars of greens-type annual bluegrass and two commercial cultivars of creeping bentgrass (‘Penncross’ and ‘Penn A-4’) were established on an experimental golf green and managed under two nitrogen (N) fertility levels (195 and 65 kg N/ha/year) over a 2-year period. Creeping bentgrass had two and three times the total root mass compared with annual bluegrass during the ﬁrst and second years of the experiment, respectively. At soil depths of 3–12 cm and below 12 cm, creeping bentgrass had three to four times the root mass compared with annual bluegrass at various times during the experiment. During the ﬁrst year of the experiment, both species exhibited greater than 50% decrease in total root mass from June to August. During the second year, creeping bentgrass total root mass decreased 10% to 15% and annual bluegrass total root mass decreased 25% to 30% over the same period. Of the two bentgrasses, ‘Penn A-4’ creeping bentgrass exhibited greater total root mass only in the second year; however, ‘Penn A-4’ exhibited greater root mass than ‘Penncross’ below 12 cm in both years. Creeping bentgrass cultivars showed greater root mass below 12 cm at 65 kg N/ha/year compared with 195 kg N/ha/year on some sampling dates in both years. Annual bluegrass cultivars showed no change in any root mass parameters in response to N rates (data not shown), but speciﬁc root length (SRL) of annual bluegrass increased under the 65 kg N/ha/year rate compared with the 195 kg N/ha/year rate, whereas SRL of creeping bentgrass was similar at both N rates. Tiller densities of both species increased under the 195 kg N/ha/ year rate. ‘Penn A-4’ exhibited higher tiller densities than ‘Penncross’ throughout the experiment and at times was equivalent to the tiller densities of the annual bluegrass cultivars. These results suggest that although creeping bentgrass increases root mass deeper in a putting green root zone mix at lower N rates (65 kg N/ha/year), annual bluegrass exhibits plasticity in speciﬁc root length in response to different N rates.

Creeping bentgrass (Agrostis stolonifera L.) and annual bluegrass (Poa annua L.) are the predominant species of grass found on golf course putting greens in the northern United States. The recent selection of high-quality inbred lines of perennial greens-type annual bluegrass has provided opportunities for comparing the competitive vigor of annual bluegrass with creeping bentgrass on putting greens (Huff, 2003(Huff, , 2004. Understanding growth habits will lead to selection of improved and more competitive cultivars of both species. Annual bluegrass has been described as having a shallow root system, but this is likely because it is often found on sites with com-pacted soils (Beard, 1973;Beard et al., 1978;Sprague and Burton, 1937;Youngner, 1959). In non-compacted soils, the root mass of annual bluegrass was comparable to root masses of colonial bentgrass and kentucky bluegrass in the top 7.5 cm or 12.5 cm of soil (Sprague and Burton, 1937). When grown in sandy loam soils at increasing bulk densities, no differences in root biomass were detected among annual bluegrass, kentucky bluegrass, and creeping bentgrass plants (Wilkinson and Duff, 1972).
The effects of temperature and other seasonal changes on the rooting of creeping bentgrass on golf greens are becoming better understood (Huang and Gao, 2000), whereas the role of fertility, particularly N fertility, has been more difficult to ascertain. Some studies have shown less root growth in bentgrass species in response to higher N rates compared with a lower N rate (Christians et al., 1979;Madison, 1962;Schlossberg and Karnok, 2001;Schmidt and Blazer, 1967). Other studies have shown increased root growth in bentgrass species in response to higher N rates compared with a lower N rate (Bell and Defrance, 1944;Kohlmeier and Eggens, 1983). Kohlmeier and Eggens (1983) found an increase in root growth at higher N rates in a study focusing on recovery from wear. Schlossberg and Karnok (2001) showed that it is important to determine the distribution of root length density with soil depth in addition to measuring total root length density to assess differences in rooting between cultivars and nutrient regimes.
Annual bluegrass growth and competitiveness is also affected by N rates (Kohlmeier and Eggens, 1983). Dest and Guillard (1987) showed that withholding N fertilization from a golf course fairway for up to 3 years reduced the amount of annual bluegrass encroachment into the stand. Although N additions have been implicated in annual bluegrass encroachment into creeping bentgrass stands, the specific effects of different N rates on annual bluegrass root growth and competitiveness is not well documented.
Understanding the distribution of root systems and tiller densities of creeping bentgrass and annual bluegrass under different N levels in sand root zones will aid in our understanding of how these species interact on golf course putting greens. The purpose of our study was to compare root distribution and tiller densities of creeping bentgrass cultivars Penncross (a medium-density, prostrate-growing cultivar released in 1954) and 'Penn A-4' (a highdensity, upright-growing cultivar released in the 1995) to three experimental cultivars of greens-type annual bluegrass throughout two growing seasons at two different N rates.

Materials and Methods
Plant materials and growing conditions. Two creeping bentgrass cultivars (Penncross, Penn A-4) and three experimental cultivars of greens-type annual bluegrass ('PSU-97-1', 'PSU-97-2', 'PSU-97-3') were seeded in monocultures into 2.75 · 4-m plots on an experimental putting green at a rate of 36.5 kgÁha -1 during late Sept. 1999. The cultivars were arranged in a randomized strip-split-plot design with three replicates. The root zone consisted of 30.5 cm of an 85% sand and 15% sphagnum peat mixture (v:v) laid over 10 cm of pea gravel conforming to the USGA specifications (Green Section Staff, 1993). The seedbed was watered to maintain a moist surface for 6 weeks after germination. Mowing height was gradually lowered and an aggressive topdressing program was implemented until a mowing height of 3.2 mm was reached in late July of the grow-in year (2000). In the grow-in year, all plots were fertilized identically using granular starter fertilizer (19N-25P-5K) (Scotts Co., Marysville, OH) followed by a liquid fertilizer containing N, phosphorus (P), and potassium (K) and a micronutrient solution to allow for optimal grow-in and establishment of the turf, resulting in 175 kg N/ha, 33 kg P/ha, and 104 kg K/ha during the grow-in season. The experiment began in Spring 2001. In 2001 (Year 1) and 2002 (Year 2), two different N rates were stripped across the plots. In both years of the experiment, the plots received applications of liquid ammonium nitrate every 10 to 20 d beginning in May at a rate of 6.1 kg N/ha and 18.3 kg N/ha for the low and high N treatments, respectively. This resulted in %65 kg N/ha/year or 195 kg N/ha/ year. Phosphorus and K were supplemented with applications of calcium phosphate and potassium chloride solution in the spring and fall resulting in 11 kg P/ha and 60 kg K/ha.

Measurements.
Tiller density and root mass were measured at three different depths on nine dates from mid-June to early November in Year 1 and on eight dates from late March to late September in Year 2. Four 3.5-cm diameter cores were taken to a depth of 25 cm from each plot (two from each N treatment) using a sharpened polyvinyl chloride (PVC) pipe every 10 to 20 d throughout each growing season. The cores were frozen within the PVC pipe at -20°C before data collection. For each core, the top 3 mm was removed using a saw for tiller density measurements and to eliminate any stem material from the root samples. The remaining core was then separated into three sections: 0-3 cm, 3-12 cm, and below 12 cm. The roots from each section were washed from the soil by hand with a 1-mm soil sieve under running water held at room temperature.
Fresh weights were recorded and subsamples of the roots were weighed and placed in 15% ethanol solution for root length analysis. The remaining roots were dried for 24 to 48 h at 70°C. Root dry weights were recorded and then samples were ashed in a muffle furnace at 605°C until all organic matter was completely combusted, typically 6 h. Ashed samples were weighed and the weight of the ash and any remaining sand in the sample were subtracted from the root dry weight. Root dry weight was then corrected for the fresh weight subsampling.
Root length was determined by staining the ethanol-preserved roots with 0.1 gÁL -1 neutral red dye (Sigma Chemical Co., St. Louis, MO) for 24 h and scanning them on a flatbed scanner using WinRhizo software (Regent Industries, Canada). Specific root length was determined after calculating the dry weight of the scanned sample using the fresh weight to ashfree dry weight ratio of the whole sample. The calculated dry weight of the scanned sample was then divided by the root length of the scanned sample resulting in the specific root length.
Tiller densities were obtained by separating the top 3 mm of the core samples into four equal parts and counting the total number of tillers in one-fourth of each core. All root and tiller measurements were averaged to obtain a plot value on each date data were collected. Table 1. Tiller densities and root mass of creeping bentgrass cultivars ('Penn A-4' and 'Penncross') and annual bluegrass cultivars ('PSU-97-1', 'PSU-97-2', 'PSU-97-3') pooled over two different N rates (65 kg N/ha/year and 195 kg N/ha/year).

Yr Cultivar
Tiller density (tillers/cm 2 ) Root mass z 0-3 cm Root mass 3-12 cm Root mass below 12 cm Total root mass Percent of total root mass in 0-3 cm Penn  Cultivar differences were measured by pooling N treatments. Analyses of variance were performed as repeated measures using the PROC MIXED procedure of the Statistical Analysis System (SAS Inc., Cary, NC). Where appropriate, differences between cultivar means at a given date of harvest or between harvest times for a given cultivar were separated by Fisher's least significance difference test at the 0.05 level. To better understand the effects of the N treatment within a species, the bentgrass and annual bluegrass data were analyzed separately.
In the repeated-measures design, the data from 2001 and 2002 were treated separately (Litell et al., 1996).

Results
Temperature patterns were similar in both years with the highest temperatures occurring during late July through late August (data not shown), and all plots sustained sufficient monocultures for sampling throughout the duration of the experimental period.
The bentgrass cultivars did not differ from each other in total root mass in Year 1. Of the annual bluegrass cultivars, 'PSU-97-3' and 'PSU-97-1' had greater total root mass than 'PSU-97-2', but they were both less than either of the two bentgrasses (Table 1). In Year 2, 'Penn A-4' had %25% more total root mass than 'Penncross', and both creeping bentgrass cultivars had more than twice the total root mass of any of the three annual bluegrass cultivars (Table 1). Annual bluegrass cultivars did not differ from one another in total root mass in Year 2. In Year 1, total root mass for all grasses was greatest on 20 June and 5 Oct. (Fig. 1). Approximately a 50% reduction in total root mass was observed throughout most of the summer compared with spring, and a second reduction was observed after the first freeze in Year 1 (Fig. 1). Approximately a 20% decrease in total root mass was observed throughout the summer of Year 2 from 18 June until 18 Aug. for all cultivars pooled, and a subsequent recovery was not observed in Year 2 (Fig. 1).
Root mass in the 0-to 3-cm depth followed a similar trend as the seasonal changes in total root mass. Among the annual bluegrasses, 'PSU-97-3' and 'PSU-97-1' had more root mass in the 0-to 3-cm depth in Year 1 than 'PSU-97-2' (Table 1). In Year 2, all three annual bluegrass cultivars had less root mass in the 0-to 3-cm depth than the creeping bentgrass cultivars with 'Penn A-4' exhibiting greater root mass than 'Penncross' ( Table  1). The annual bluegrass selections had a higher percentage of their total root mass in the 0-to 3-cm depth compared with the two creeping bentgrass cultivars. In Year 1, 'PSU-97-1' had a lower percentage (75%) of total root mass in the 0-to 3-cm depth than 'PSU-97-2' (79%) and 'PSU-97-3' (82%). The majority of total root mass for all grasses was found in the 0-to 3-cm depth throughout the experiment, because 75% to 85% and 60% to 70% of the total root mass was found in the 0-to 3-cm depth for the annual bluegrass cultivars and the creeping bentgrass cultivars, respectively (Table 1).
In both years of the experiment, bentgrass cultivars had significantly more root mass in the 3-to 12-cm depth than the annual bluegrass cultivars with approximately three times as much root mass in this zone in Year 1 and four times as much root mass in this zone in Year 2. No differences in root mass were found among cultivars within either species at 3 to 12 cm (Table 1). Root mass in the 3-to 12-cm depth was greatest on 20 June, 7 July, and 5 Oct. in Year 1 and exhibited a summer decline from 24 July until 21 Sept. (Fig. 2). Like with total root mass in Year 1, a fall recovery was observed in the 3-to 12-cm depth followed by a decline in the late fall for all cultivars (Fig. 2). In Year 2, the root mass in the 3-to 12-cm depth was relatively constant throughout the growing season and the pattern of growth was similar to that of total root mass.
'Penn A-4' had greater root mass below 12 cm compared with 'Penncross'. The difference in root mass below the 12-cm depth was the only consistent difference in root mass between 'Penn A-4' and 'Penncross' at any depth throughout both years of the experiment (Fig. 3). On three occasions in Year 1, both bentgrass cultivars showed more root mass below 12 cm in the 65 kg N/ha/year compared with the 195 kg N/ha/year treatment. Root mass means across all harvest dates for 'Penn A-4' were 0.025 g and 0.022 g for the 65 kg N/ha/year and 195 kg N/ha/year treatments, respectively, and 0.017 and 0.014 g for the 'Penncross' 65 kg N/ha/year and 195 kg N/ha/year treatments, respectively. The annual bluegrass selections had very little root mass below 12 cm of depth, and in the summer months, the root mass in this zone approached zero (data not shown). In Year 1, a pattern of root mass decline and recovery was observed in this section of the root zone that was similar to that observed in the zones above it (Fig. 3). This pattern was not observed in Year 2 because the 'Penn A-4' root mass declined from 18 June to the end of the sampling period, whereas 'Penncross' exhibited no change across the same sampling dates.
Specific root lengths of cultivars and selections within the two species were pooled because no differences were observed. Creeping bentgrass had similar specific root lengths in both the 195 kg N/ha/year and 65 kg N/ha/ year treatments (Fig. 4). Specific root lengths of creeping bentgrass in the 195 kg N/ha/year and 65 kg N/ha/year treatments were not different from the specific root lengths of annual bluegrass in the 195 kg N/ha/year treatment but were significantly lower than the specific root length of annual bluegrass in the 65 kg N/ha/year treatment.
An increase in tiller density for 'PSU-97-3' was observed from the first harvest on 20 June to 7 Aug. and a second increase was observed on the 1 Nov. harvest date in Year 1 (Fig. 5). During the fall of Year 2, 'PSU-97-3' showed a slight reduction in tiller density compared with previous harvests, whereas 'Penn A-4' had an increase in tiller density throughout the season (Fig. 5). In Year 1, the 195 kg N/ha/year treatment produced higher tiller densities for the creeping bentgrass cultivars (47.7 tillers/cm 2 ) than the 65 kg N/ha/ year treatment (42.6 tillers/cm 2 ) (data not shown). In Year 2, there was no overall N effect on tiller density, although there was an interaction between N rate and day of harvest as tiller density increased with the 65 kg N/ ha/year treatment on the 25 Apr., 18 Aug., and 25 Sept. harvest dates (data not shown). There was no cultivar by N rate interaction for annual bluegrass, and all three selections were pooled. The 195 kg N/ha/year rate had increased tiller densities over the 65 kg N/ha/ year rate throughout the experiment (data not shown).

Discussion
The purpose of this study was to provide a side-by-side comparison of creeping bentgrass and greens-type annual bluegrass rooting distribution and tiller densities at two N rates under a putting green mowing height throughout two growing seasons. Tiller density has been implicated in the competitiveness of grasses on golf greens (Harivandi et al., 2008). In the present study, the greens-type annual bluegrass cultivars had the highest tiller densities, whereas the Penncross cultivar of creeping bentgrass had the lowest. In addition to having higher tiller densities, cultivars  of creeping bentgrass entering the commercial market during the 1990s have been reported to develop a more extensive root system (Beard et al., 2001) as was observed with 'Penn A-4' in this study. The high tiller densities observed in some of the new cultivars of bentgrass may be associated with finer leaf texture and stem textures (Beard et al., 2001) and not necessarily the result of a decrease in belowground carbon allocation. The finer-textured bentgrass cultivars may have a lower aboveground carbon demand per tiller than coarser-textured cultivars, thus allowing for similar belowground carbon allocation. High tiller densities may also allow bentgrass cultivars to expand into disturbed areas such as ball marks or aeration holes through increased vegetative propagation. A grass with higher tiller numbers would have a greater number of potential starting points for stolons and daughter tillers to begin to exploit the disturbed area than a grass with lower tiller densities. Although the 195 kg N/ha/year rate increased tiller densities of both 'Penncross' and 'Penn A-4' creeping bentgrass in the first year of the experiment, it also increased tiller densities of the annual bluegrass selections in both years of the experiment. Thus, higher N rates may not lead to a reduction of annual bluegrass invasion, at least on the basis of tiller density.
In the present study, creeping bentgrass produced greater total root mass and greater root mass deeper in the putting green root zone than annual bluegrass. Root masses peaked in spring and fall of the first year and declined over the summer months. Summer root decline of turfgrasses has previously been observed using both rhizotron and traditional core sampling techniques (Kurtz and Kneebone, 1980;Ralston and Daniel, 1972;Wang et al., 1998;Yelverton, 1999). Huang and Liu (2003) showed that summer root decline in creeping bentgrass was associated with a decrease in the production of new roots and an increase in the death of existing roots. One limitation of the previously cited studies is root distribution at different depths was not examined, and this can be important in determining differences in root growth between cultivars (Schlossberg and Karnok, 2001). Summer root decline adversely affects plants by limiting water and nutrient supply to the shoots (Kramer, 1983). Although all of the cultivars and selections of grasses in our study showed significant summer root decline, 'Penn A-4' was able to maintain the highest total root mass and root mass at deeper soil depths, likely leading to improved stress tolerance during the warmer months of the growing season. This ability may confer a competitive advantage to the 'Penn A-4' on golf greens if water becomes limiting in the late afternoon. If 'Penn A-4' has a greater ability to survive heat stress, and possibly intercept more nutrients throughout the root zone, it would have a competitive advantage over annual bluegrass when water and nutrients are less available or when they are only present deeper in the root zone. The deeper root system of creeping bentgrass, as compared with annual bluegrass, may result in delayed stomatal closure and lead to greater net photosynthesis. Such a response would likely increase summer heat tolerance of creeping bentgrass compared with annual bluegrass.
The root mortality that occurs during summer root decline may be the result of inhibition of carbon supply from shoots to roots at high soil temperatures (Ruter and Ingram, 1990). In creeping bentgrass, Liu and Huang (2002) found that turf plants maintained at a higher cutting height (4 mm) had a decreased rate of root mortality when compared with plants maintained at lower cutting heights (3 mm), indicating the importance of mowing height in maintaining a healthy root system. Closely mowed turfgrasses with limited leaf area may be constrained by their ability to fix a sufficient amount of carbon to support vigorous growth (Huang and Gao, 2000).
The N in this experiment was applied in liquid form on a regular basis and the plots were watered after fertilizers were applied to maximize root uptake of N. The increase in root mass below 12 cm at the 65 kg N/ha/year rate for creeping bentgrass in Year 1 is evidence that this species is maximizing N uptake by exploring a greater portion of the root zone. Bowman et al. (1998) showed that a cultivar of creeping bentgrass with a deeper, more extensive root system was able to capture more N than a shallower rooted cultivar. The more extensive creeping bentgrass root mass observed below 12 cm in the 65 kg N/ ha/year treatments during Year 1 occurred on harvest dates in June and October but not in July and August. The increased resource allocation to root growth at deeper depths in the 65 kg N/ha/year treatment may only occur when carbon is not limiting. In the summer months, carbon is limiting in closely mowed turfgrass as a result of increased respiration rates and limited photosynthesis (Huang and Gao, 2000;Huang and Liu, 2003). Our results indicate that during the summer period, the plants no longer alter their root growth to increase N acquisition under the 65 kg N/ha/ year treatment. Another possible explanation is that as the root mass in the deeper portion of the soil declined in summer, the differences between the 195 kg N/ha/year and 65 kg N/ha/ year treatments were too small to be detected. Annual bluegrass is most competitive during times when temperatures are moderate, like in the spring and fall (Lush, 1988). The limited root system of annual bluegrass may be a primary cause for its lack of competitiveness in the warmer summer months. The ability of plants to spatially and temporally access nutrient supplies is an important factor in determining nutrient uptake and a determining factor in plant competition (Crick and Grime, 1987;Hodge et al., 1999;Jackson and Caldwell, 1996). Plant growth on golf greens is severely limited by low mowing heights (Hull, 1992). It is possible that the limited root system of annual bluegrass is a result of evolution under low mowing heights.
Another explanation for the competitive nature of annual bluegrass on creeping bentgrass golf putting greens is its ability to alter its specific root length in response to nutrient availability. Specific root length often increases in response to low nutrient availability (Fitter, 1985). The increase in specific root length could also have implications in the carbon balance of the plant. Although thicker roots are more costly to produce, they have greater transport capacity (Fitter, 1987), possibly aiding in water acquisition. Because the N in our study was applied uniformly to the soil surface, the increase in specific root length of the annual bluegrass may have been more apparent because a greater percentage of annual bluegrass root mass exists in the upper soil layers. Plants with higher specific root lengths, or finer roots, tend to have more rapid proliferation of roots than plants with thicker roots such as in tussock grasses (Eissenstat and Caldwell, 1987). High specific root length is associated with roots growing into nutrient rich patches (Huang, 1999;Huante et al., 1998;Lariguaderie and Richards, 1994), and carbon allocation needed per unit of root length is lower for plant roots with a higher specific root length (Eissenstat, 1992;Huang and Eissenstat, 2000). Low mowing heights severely limit the energy available for root growth and have been shown to limit the total root mass in creeping bentgrass (Hull, 1992). The ability of annual bluegrass to increase its specific root length in response to the 65 kg N/ha/year rate observed in the present study may allow an increase in total root length without increasing its root mass, thereby increasing nutrient acquisition with less belowground carbon allocation than other grasses. This would allow the annual bluegrass to acquire nutrients more efficiently than creeping bentgrass in a carbon-limited environment.
Findings from the present experiment confirm a long-held belief that annual bluegrass has fewer total roots and fewer roots deeper in the soil than creeping bentgrass when grown under golf green management conditions. Both species experienced a decline in rooting at all depths throughout the summer months as a result of high temperatures, which can be attributed by carbon deficits caused by a reduction in photosynthesis and a corresponding increase in respiration of roots (Huang and Gao, 2000;Huang and Liu, 2003). Finally, creeping bentgrass responded to low N in soil by increasing root mass below 12 cm, whereas annual bluegrass had an increase in specific root length, possibly improving competitiveness under carbon-limited environments like a golf green mowed between 2.5 and 4 mm.