Synchronous culture

A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage.
All cells in the synchronously growing culture divide at a time, they yield a Zig Zag pattern of growth curve.
As numerous factors influence the cell cycle (some of them stochastic) normal cultures have cells in all stages of the cell cycle. Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be easily estimated, and quantitative experimental results can simply be divided in the number of cells to obtain values that apply to a single cell. Synchronous cultures have been extensively used to address questions regarding cell cycle and growth, and the effects of various factors on these.
Methods.
Synchronous cultures can be obtained in several ways: