During his undergraduate career at Swarthmore College, Provencio worked as a lab technician in Steven M. Reppert's research laboratory at Massachusetts General Hospital, where he was introduced to the field of circadian biology.
During his undergraduate career at Swarthmore College, Provencio became interested in neuroscience while studying crayfish, cockroaches, and fireflies under Jon Copeland. He also worked as a lab technician in Steven M. Reppert's research laboratory at Massachusetts General Hospital, where he was introduced to the field of circadian biology.