An appropriate dilution, or several dilutions within the estimated appropriate range, is spread using sterile technique on the agar plate, which is then incubated under the appropriate conditions for growth until individual colonies appear.
An appropriate dilution, or several dilutions within the estimated appropriate range, is spread using sterile technique on the agar plate or plated automatically using a plating device such as a spiral plater. The agar plate is then incubated under the appropriate conditions for growth until individual colonies appear.