Charlotte was born in Nicosia on 28 June the eldest child of's paternal grandparents were King Janus of Cyprus and Charlotte de Bourbon-La Marche.
Charlotte was born in Nicosia on 28 June 1444, the eldest and only surviving daughter of King John II of Cyprus and Helena Palaiologina. Her paternal grandparents were King Janus of Cyprus and Charlotte de Bourbon-La Marche.