familial mediterranean fever ( fmf , mim 249100 ) is an autosomal recessive disease characterized by recurrent episodes of fever accompanied with topical signs of inflammation mainly in the peritoneal , pleural , and articular cavities . the disease is caused by mutations in the mefv gene on 16p13 , which is composed of 10 exons and encodes 781-amino - acid protein known as pyrin / marenostrin involved in the regulation of inflammation . to date , more than 150 gene alterations ( mutations / polymorphisms ) located in the mefv gene have been identified . the m694v , v726a , m694i , m680i mutations in exon 10 are the most frequent among all populations , and are associated with the most severe clinical pictures with the risk of aa amyloidosis . in morocco , we report on two patients , who were not on colchicine prophylaxis , and developed chronic renal failure because their fmf statute was not known before they developed renal amyloidosis . a 16-year - old boy was referred for end - stage renal failure caused by renal amyloidosis of the aa type . he had been subject , for 13 years , to recurrent episodes of fever lasting two to three days and accompanied by abdominal and joint pains . he is consanguineous in the first degree , the third of four children , with a positive family history of recurrent episodes of fever , less severe in his older sister of 18 years old [ figure 1 ] . molecular genetic testing for fmf was performed by dna sequencing of exon 10 of the mefv gene , as previously reported . as a result , molecular analysis of his younger sister allowed us to comfort her about her status of normal homozygote after an episode of articular pain that alarm her parents because of the familial history of fmf . she has been having periodic episodes of high fever , usually lasting less than 3 days , at 1-to 3-month intervals since she was 22 years old . the patient had no medical follow - up for several months . during a severe episode , she was admitted to hospital and diagnosed with aa amyloidosis responsible of a chronic renal failure . the symptomatic occurrence of periodic fevers with spontaneous improvement within 3 days suggested a possible diagnosis of fmf . after obtaining informed consent , dna sequencing of exon 10 of the mefv gene was performed . a 16-year - old boy was referred for end - stage renal failure caused by renal amyloidosis of the aa type . he had been subject , for 13 years , to recurrent episodes of fever lasting two to three days and accompanied by abdominal and joint pains . he is consanguineous in the first degree , the third of four children , with a positive family history of recurrent episodes of fever , less severe in his older sister of 18 years old [ figure 1 ] . molecular genetic testing for fmf was performed by dna sequencing of exon 10 of the mefv gene , as previously reported . as a result , molecular analysis of his younger sister allowed us to comfort her about her status of normal homozygote after an episode of articular pain that alarm her parents because of the familial history of fmf . she has been having periodic episodes of high fever , usually lasting less than 3 days , at 1-to 3-month intervals since she was 22 years old . the patient had no medical follow - up for several months . during a severe episode , she was admitted to hospital and diagnosed with aa amyloidosis responsible of a chronic renal failure . the symptomatic occurrence of periodic fevers with spontaneous improvement within 3 days suggested a possible diagnosis of fmf . after obtaining informed consent , dna sequencing of exon 10 of the mefv gene was performed . a 16-year - old boy was referred for end - stage renal failure caused by renal amyloidosis of the aa type . he had been subject , for 13 years , to recurrent episodes of fever lasting two to three days and accompanied by abdominal and joint pains . he is consanguineous in the first degree , the third of four children , with a positive family history of recurrent episodes of fever , less severe in his older sister of 18 years old [ figure 1 ] . molecular genetic testing for fmf was performed by dna sequencing of exon 10 of the mefv gene , as previously reported . as a result , molecular analysis of his younger sister allowed us to comfort her about her status of normal homozygote after an episode of articular pain that alarm her parents because of the familial history of fmf . she has been having periodic episodes of high fever , usually lasting less than 3 days , at 1-to 3-month intervals since she was 22 years old . the patient had no medical follow - up for several months . during a severe episode , she was admitted to hospital and diagnosed with aa amyloidosis responsible of a chronic renal failure . the symptomatic occurrence of periodic fevers with spontaneous improvement within 3 days suggested a possible diagnosis of fmf . after obtaining informed consent , dna sequencing of exon 10 of the mefv gene as a mediterranean population , maghrebians are particularly prone to develop fmf . the department of medical genetics at the national institute of health in rabat , considered a major medical genetics pole in morocco , has taken interest in this pathology for a decade . this interest is motivated by two elements : the relatively high frequency of the disease among north african populations , which is estimated at 1% and the high level of consanguineous marriages in our society ( 15.25% ) that leads to a high incidence of autosomal recessive disorders . we performed molecular diagnosis of fmf by screening for mutations of exons 10 and 2 , as previously described . we also worked on the characterization of the mutational profile of this disease among the moroccan population . as such , we have recently reported data on mefv gene mutations causing fmf among moroccans patients . the most frequent mutation in moroccan patients is m694v ( 47 % ) , followed by m694i ( 32 % ) , a744s ( 6.5 % ) , m680l ( 4 % ) , m694del ( 2% ) , and e148q ( 6.5 % ) . a positive response to colchicine treatment makes a good case for the disease , but only genetic studies are considered a clinical proof of the disease . early diagnosis of fmf is crucial to avoid year - long roundtrip visits to the hospital to get a diagnosis and even sometimes undergo unnecessary abdominal surgery because of the symptom of abdominal pain . the early and accurate diagnosis of fmf is important all the more so as colchicine has proved to be an effective and cheap treatment , helping to reduce significantly the frequency and duration of attacks and to delay renal complication . in spite of all these clinical and epidemiological data , fmf remains a little - known and largely underestimated pathology in morocco 's daily medical practice , which does not allow patients to get a diagnosis in specialized centers . the two clinical cases reported highlight the importance of an early diagnosis of fmf to introduce the appropriate therapeutic to prevent renal amylodoisis and for genetic counseling of families . the first case reported here is homozygous for the m694v , a dominant mutation in our population associated with a severe phenotype . his sister , who has the same genotype , has a less severe clinical picture . this intrafamilial variability in the clinical expression can be explained by the action of modifying genes in the mefv gene . the v726a mutation is the second most frequent mutation in arabs from middle east , in armenians and turks , while it was nonexistent in our population . the data reported by our preliminary study , and by the two cases , are prone to bring moroccan clinicians attention to the relative frequency of this disease in morocco . we encourage them to consider this pathology in their daily practice and to work in concert with geneticians and epidemiologists to run wider studies to characterize the clinical and molecular profile of fmf in our country and contribute to take better care of our patients . moreover , the present study proves the importance of early diagnosis of fmf , both to start rapidly treatment with colchicine and avoid renal amyloidosis , and to provide genetic counseling to families .
familial mediterranean fever ( fmf , mim 249100 ) is an autosomal recessive disease affecting mainly patients of the mediterranean basin . 
 it is an autoinflammatory periodic disorder characterized by recurrent episodes of fever and abdominal pain , synovitis , and pleuritis . 
 the major complication of fmf is the development of renal aa amyloidosis . 
 treatment with colchicine prevents the occurrence of recurrent seizures and renal amyloidosis . 
 the disease is caused by mutations in the mefv gene . 
 we report here the cases of two unrelated patients , who have been late diagnosed with fmf complicated by renal amyloidosis . 
 we focus on the importance of early diagnosis of fmf , both to start rapidly treatment with colchicine and avoid renal amyloidosis , and to provide genetic counseling to families .