it is difficult to adjust the depth of anaesthesia in invasive procedures outside the operating room . although target - controlled infusion ( tci ) or total intravenous anaesthesia ( tiva ) infusion pumps enable rapid adjustments in sedation level , optimal conditions may not be available everywhere or every time . although superficial anaesthesia in children prevents successful performance , very deep anaesthesia or sedation may cause respiratory depression and other related side effects . a large number of sedatives and anaesthetic drugs are used in the uge process in children . the effects of ketamine , one of the most commonly used agents in uge of children , are rapid , but short ; ketamine offers quick recovery and has wide confidence intervals 2,3 . when used for sedation and anaesthesia , ketamine causes dissociative anaesthesia characterized by amnesia and analgesia 4,5 . however , side effects such as aspiration , stridor , laryngospasm and after - sedation nausea have been reported 6,7 . in addition , there may be some effects called emergence reactions such as nightmares , delirium , excitation and physical aggression 8 . ketamine is used in combination with benzodiazepines ( especially midazolam ) to reduce the frequency of these side effects 2,3,911 . it has sedative , hypnotic , anxiolytic and anticonvulsant properties and causes anterograde amnesia 12,13 . it provides rapid and reliable unconsciousness and consciousness with its sedative , hypnotic and amnestic effects . however , its analgesic effect is weak 14 . despite its use by anaesthesiologists in many centres , it can cause hypotension and respiratory depression at high doses 15,16 . the use of propofol in combination with other sedatives provides a synergistic effect and enables the use of lower doses 17,18 . fentanyl is used in combination with propofol as an adjuvant to allow for effective use of propofol at lower doses 14 . the best sedation procedure with minimal side effects providing the best comfort both for the patient and for the endoscopist during the uge of children is still under discussion . the primary aim of this study was to investigate the efficiency and safety of midazolam plus ketamine versus fentanyl plus propofol administered to children undergoing uge and to determine the most appropriate sedation protocol . as a secondary aim this prospective , randomized , single - blind study was carried out in the department of pediatric gastroenterology , hepatology and nutrition at the kanuni training and research hospital . the study was carried out after receiving approval from the local ethics committee ( registry url : 2015/18 identifier : trabzon kanuni training and research hospital clinical research ethics committee ) and informed parental consent in accordance with the declaration of helsinki . all the patients were american society of anesthesiologists ( asa ) physical status i or ii 19 . patients with respiratory tract infections , glaucoma , psychosis , porphyria , hypertension , metabolic or neurologic diseases , increased intracranial pressure and intracranial mass and patients known to be allergic to the drugs used were excluded from the study . the procedures were applied in the endoscopy unit by a paediatric endoscopist and an endoscopy nurse . follow - up was performed by another nurse in the recovery room after the procedure . all procedures were performed using the olympus gif - h180 device ( olympus corporation , tokyo , japan ) in the left lateral position . after 6-h fasting 20 , blinded patients were randomized 1  :  1 to receive midazolam plus ketamine combination in group a or fentanyl plus propofol combination in group b for sedation . the effectiveness of sedation during the procedure was evaluated according to a modified ramsay sedation score ( rss ) ( table 1 ) . a score from 16 rss of 5 or above was aimed at for a comfortable procedure in our study . ramsay scale for the assessment of the level of sedation the patients were administered a spray of lidocaine 10% ( xylocaine spray ; astrazeneca , silk rod , uk ) as a topical pharyngeal anaesthesia before sedation . an intravenous midazolam ( dormicum , 1  mg / ml , 5  ml ; deva holding , istanbul , turkey ) bolus dose 0.1  mg / kg ( maximum 4  mg ) was administered to group a. two minutes later , ketamine ( ketalar , 50  mg / ml , 10  ml ; pfizer , sandwich , uk ) bolus dose 1  mg / kg was administered intravenously . patient responses to verbal and tactile stimuli were evaluated 2  min after the administration of ketamine . ketamine 0.5  mg / kg ( maximum of 2  mg / kg ) was added at 2-min intervals if adequate sedation was not achieved initially . if the patients were agitated after the start of the procedure , a single dose of ketamine 0.5  mg / kg was administered . intravenous fentanyl ( fentanyl , 0.05  mg / ml , 10  ml ; johnson & jonhson , turnhout , belgium ) bolus dose 1  g / kg was administered to group b. two minutes later , propofol ( propofol - lipuro , 10  mg / ml , 20  ml ; b. braun , melsungen , germany ) bolus dose 1  mg / kg was administered intravenously . patient responses to verbal and tactile stimuli were evaluated 2  min after the administration of propofol . propofol 0.5  mg / kg was added at 2-min intervals if adequate sedation was not achieved . if the patients were agitated after the procedure started , a single dose of propofol 0.5  mg / kg was administered . all patients were monitored for peripheral oxygen saturation , heart rate ( hr ) , respiratory rate and rss during the procedure . oxygen ( 2  l / min ) by a nasal cannula was administered to all patients during the procedure . cardiac arrest , apnoea and laryngospasm were assessed as major complications , whereas hypoxia ( peripheral oxygen saturation < 90% during 60  s ) , tachycardia ( defined as 30% more than the average hr by age ) , bradycardia ( 30% less than the average hr by age ) , increase in oral secretions ( copious oral secretions requiring suctioning ) , flushing , coughing and vomiting were assessed as minor complications . the completion of the procedure without any major complications indicated the success rate of sedations . the modified aldrete score was determined by scoring from 0 to 10 according to the patient s activity , oxygen saturation , consciousness , respiration and circulation 22 . patients with an aldrete score of 9 or greater were discharged from the endoscopy unit . the time between the end of the process and departure from the endoscopy unit ( the recovery time ) was recorded . complications during the recovery time such as double vision , dizziness , nausea and vomiting were recorded . hallucinations , agitation and emergence reactions were evaluated and recorded . emergence reactions included severe agitations such as nightmares , hallucinations and delirium observed during the recovery time 11 . meansd were used as descriptive statistics ; an independent two - sample t - test was used for normal distribution in the comparison of two groups and the mann the data were evaluated using the spss , version 13.0 ( spss inc . , chicago , illinois , usa ) statistical package program . five parents did not agree to participate in the study and seven patients were excluded from the study because of missing data . therefore , 238 patients were evaluated ( 119 patients in group a and 119 patients in group b ) . the median age of the patients was 12 years ( range : 417 years ; mean sd : 12.213.38 years ) and the median weight was 40  kg ( range : 1795  kg ; meansd : 41.9215.24  kg ) . other indications were chronic dyspepsia ( 19.3% ) , reflux - related symptoms ( 14.2 ) , suspected coeliac disease ( 2.1% ) and chronic diarrhoea ( 1.4% ) . the endoscopy process started without an additional dose of the drug for 118 patients ( 99.1% ) in group a and for 101 patients ( 84.8% ) in group b ( p=0.001 ) . six patients in group a were agitated during the endoscopic procedure and were administered additional doses of ketamine ( 5.0% ) and 45 patients in group b ( 36.1% ) were administered additional propofol ( p=0.001 ) . the average dose of ketamine administered to the patients in group a was 1.030.15  mg / kg and the average dose of propofol administered to the patients in group b was 1.460.55  mg / kg ( table 2 ) . the endoscopic procedure was not discontinued for any of the patients in group a , but for one patient in group b ( 0.8% ) , the endoscopic procedure had to be discontinued as the patient was agitated during the procedure . evaluation of patients during and after endoscopy the average endoscopic procedure duration was about 5  min ( range : 38  min , meansd : 4.550.75  min ) . no difference was recorded in the endoscopic procedure times between the two groups ( table 2 ) . rss measured during the intervention ( after the endoscope passed the oesophagus and until the end of the procedure ) was significantly higher in group a than in group b ( meansd : 4.941.17 vs. 2.981.88 , p<0.01 ) . there were 41 patients with an rss score of 6 in group a ( 34.5% ) and five patients in group b with an rss score of 6 ( 4.2% ) . there were only six patients with an rss score of one in group a ( 5.0% ) , but 52 patients with an rss score of one in group b ( 43.7% ) ( fig . a post - hoc power analysis showed that from the data obtained from our study , power was calculated to be 99% , indicating a significant difference in rss between the groups . no patients developed major complications such as cardiac arrest , apnoea or laryngospasm during the procedure . a total of 68 patients ( 28.6% ) developed minor complications ; 27 patients ( 22.7% ) in group a and 41 patients ( 34.5% ) in group b developed complications [ p=0.044 , odds ratio ( or ) : 0.55 , 95% confidence interval ( ci ) : 0.310.98 ] ( fig . 2 ) . vomiting , cough , tachycardia and increased oral secretions were observed more often in group a , but flushing , hypoxia and bradycardia were observed more frequently in group b. there was a statistically significant difference only in the occurrence of bradycardia among these complications ( one patient in group a and 11 patients in group b ) ( p=0.003 , or : 0.08 , 95% ci : 0.010.65 ) ( fig . oxygen saturation of six patients developing hypoxia improved in a short time with additional oxygen ( 46  l / min ) and the positioning of the airway . the endoscopic procedures were completed successfully with the improvement in oxygen saturation and the endoscopic procedures were not stopped in any patient . although an increase in secretion is a risk in aspiration and laryngospasm , these complications were observed in none of our patients . symptoms suggestive of aspiration pneumonia were not observed in the recovery room after the procedure or during outpatient follow - up of these patients . bradycardia did not cause any haemodynamic disorders such as hypotension in any of the patients . normal pulse rate was obtained after applying 0.02  mg / kg atropine ( atropine sulfate biofarma , 0.25  mg / ml , 1  ml ; biofarma , istanbul , turkey ) and the endoscopic procedures were completed successfully . all endoscopic procedures were completed successfully in group a. an endoscopic procedure had to be terminated in group b as the patient was agitated during the process . no correlation was discovered between weight and sex of the patients and complications during the endoscopic procedures . the average recovery time was 55  min ( range : 10145  min , meansd : 56.6530.37  min ) . the recovery time of group a was significantly higher than that of group b ( 78.8525.47 vs. 34.4614.50  min , p<0.01 ) . complication rates of group a ( 110 patients , 92.4% ) were significantly higher than those of group b ( 48 patients , 40.3% ) ( p=0.001 , or : 18.07 , 95% ci : 8.3539.12 ) ( table 2 ) . headache , dizziness , hallucinations , double vision , anxiety , nausea and vomiting were significantly more prevalent in group a than group b ( fig . emergence reactions were observed in six patients ( 5.0% ) in group a , but none in group b. no correlation was found between sex and age of the patients and complications during the recovery . in this study , effective sedation was provided with midazolam plus ketamine ( midazolam  ketamine ) and fentanyl plus propofol ( fentanyl  propofol ) when performing an upper gastrointestinal uge on children . however , the recovery time was longer in the midazolam  ketamine group . however , the fentanyl  propofol group was more comfortable in the recovery period in terms of complications . it may not always be suitable for general anaesthesia everywhere because of equipment and physical conditions . mature teenagers can tolerate the uge under mild sedation , but children , especially those younger than 10 years of age , may not be cooperative and may not tolerate this procedure . . however , the need for deeper sedation during procedures and anatomical differences contribute towards the development of complications . higher rates of complications associated with sedation are encountered in children compared with adults 23 . the most appropriate sedative drugs and experienced personnel these studies showed that ketamine plus midazolam is more effective and has fewer side effects than ketamine alone 2,3,11 . in our previous study , we used 0.1  mg / kg midazolam and a 0.5  mg / kg low dose of ketamine in uge for children ; the success rate was 97.42% 24 . 11 reported that the initial dose of ketamine should be 11.5  mg / kg and its efficacy is not sufficient at lower doses . in the present study , we used 1.03  mg / kg ketamine , on average , and achieved 100% success , which supports their view . the ratio of unsuccessful sedation reported in previous studies ranged between 0 and 0.4% 2527 . however , 14.2% of the children undergoing uge experienced extreme discomfort when propofol was used alone 28 . it has been stated that propofol has no analgesic properties and it may be combined with analgesics for all painful procedures 29 . propofol in combination with midazolam or fentanyl was shown to provide greater comfort during the procedure and to lead to fewer side effects 1,16 . a combination of these drugs enables the use of lower doses of propofol 15 . in the study carried out by tosun et al . 
 1 , 1  g / kg fentanyl and 1.2  mg / kg propofol were administered initially , and 50% of the patients had to be administered additional doses of propofol . in that study , the average amount of propofol administered to the patients was 2.48  mg / kg . in our study , 52.9% of the patients who were administered 1  mg / kg propofol at the beginning of sedation had to be administered an additional dose of propofol ( 1.46  mg / kg , on average ) . despite the high success rate of the procedure in these patients , their comfort during the process was significantly lower than that for the patients in the midazolam plus ketamine group ( the rss of 43.7% of the patients was 1 ) . we believed that the dose of propofol and fentanyl was lower because of the absence of tci for tiva in our hospital conditions ; thus , the comfort of this group could not reach the level of the other group . ketamine increases airway secretions and can cause laryngospasm 30 . in a meta - analysis , 3.9% of the serious airway or respiratory side effects were associated with ketamine sedation in children 31 . in particular , administration of high doses of ketamine ( initial dose of > 2.5  mg / kg ) and accompanied use of benzodiazepine increase the risk of complications for children under the age of 2 years . although mild respiratory side effects , such as a temporary decrease in saturation , have been recorded in sedation with propofol , major respiratory complications , such as severe hypoxia , apnoea and total airway obstruction , have rarely been reported 32 . in the study by larsen and colleagues , minor respiratory complications occurred in 35% of the children under the age of 1 year and in 12.5% of the children under the age of 10 years 27 . 16 reported that 14 patients ( 1.7% ) who developed laryngospasm were younger than 4 years of age . in our study , hypoxia was more frequent in the fentanyl  propofol group . we believed that respiratory depression because of propofol caused hypoxia . because positioning the airway led to stimuli of respiration , desaturation of oxygen however , the finding of hypoxia was not statistically significant , and it soon improved in both groups after positioning the airway and providing additional oxygen . in our study , no major side effects such as laryngospasm developed . however , it can cause a moderate sinus bradycardia when used with other sedative drugs . it was reported that the combination of fentanyl with those other drugs may cause bradycardia , especially in children younger than 2 years of age 14 . the reason why bradycardia is associated with the use of fentanyl is not known exactly , but it is considered that the drug suppresses the vagal afferent neurons coming from the nucleus to the heart through the -opioid receptors , thereby causing bradycardia 33 . in our study , significantly higher bradycardia was observed in the fentanyl  propofol group ( 11 patients , 9.2% ) compared with the midazolam  ketamine group ( one patient , 0.8% ) . patients who developed bradycardia had to be administered atropine , but no circulatory complications resulting in the need to stop the procedure occurred . in our study , the rate of complications during the recovery was significantly higher in the midazolam  ketamine group . agitation and emergence reactions are significant side effects observed during recovery in ketamine - based sedation . in the meta - analysis carried out , although side effects occurred in 30% of the adults , they only occurred in 1.4% of the children 31 . the incidence of emergence reactions increases , especially when ketamine is used at high doses , when a fast injection ( < 1 minute ) is administered and when excessive visual or verbal stimuli exist during the recovery 2,4 . in our previous study , we used 0.5  mg / kg ketamine and the emergence reaction was 1.2% . however , in the present study , we used 1.03  mg / kg ketamine and the emergence reaction was 5% 24 . several studies in the literature have reported that fentanyl and propofol reduce the incidence of emergence reactions 3436 . in our study , similarly , none of the patients administered the fentanyl  propofol combination developed emergence reactions . ketamine - associated vomiting differs with the procedure and it occurs in 3.5 to 28.4% of all cases 37 . after endoscopy , nausea and vomiting during the recovery period cause unrest , especially in parents . motamed et al . 
 4 reported that vomiting occurred in 17.6% of the children who underwent uge procedures . some studies have reported that administration of atropine along with ketamine sedation reduces vomiting , but there is no consensus on this issue 3739 . in our study without prophylactic atropine , nausea was found in 62.2% of the patients in the midazolam  ketamine group and vomiting was found in 33.6% of these patients , which is higher than the findings of previous studies . propofol has been shown to have antiemetic effects in different surgical procedures 4043 . in our study , significantly less nausea and vomiting was also observed in the fentanyl in addition , patients younger than 4 years of age were excluded from the study . therefore , probable complications in a younger age group , especially in children under the age of 1 year , could not be considered . the exclusion of younger children and of patients with asa status more than ii are a significant limitation of the study as young and sicker children have a higher need for sedation . as an example , neurological children ( most of whom are asa iii ) represent a significant proportion of patients undergoing digestive endoscopy and we need more data on safe sedation in these diseases . the administration of propofol by a tci device and the evaluation of sedation level by a bispectral index device are more reliable than the conventional method for the regulation of sedation and evaluation of comfort . if we had used tci and bispectral index devices , we would have obtained more objective results . our study showed that midazolam  ketamine and fentanyl  propofol combinations provide effective sedation of children during uge . in children , uge procedures can be quite comfortable when using the midazolam  ketamine combination . the wide margin of safety of the ketamine dose and the absence of cardiopulmonary suppressive property as well as the anxiolytic and analgesic effects in children make this combination ideal . however , adverse effects related to ketamine were observed during the recovery , which causes concern in parents . in our study , with no antidote , more side effects our study was prospective , and all the side effects were recorded ; this might have led to a higher rate of side effects compared with the findings from previous studies . although the uge procedure is less comfortable with the fentanyl  propofol combination in comparison with the midazolam ketamine combination , the recovery time is less with fentanyl  propofol and there are fewer side effects during the recovery period .
purposewe aimed to compare the efficacy and safety of midazolam plus ketamine versus fentanyl plus propofol combination administered to children undergoing upper gastrointestinal endoscopy ( uge ) and to determine the most appropriate sedation protocol.materials and methodsthis prospective , randomized , single - blind study included patients between the ages of 4 and 17 years who underwent uge for diagnostic purposes . 
 patients were divided randomly into groups a ( midazolam  ketamine combination , n=119 ) and b ( fentanyl plus propofol combination , n=119 ) . 
 the effectiveness of the sedation and complications during the procedure and recovery period were recorded.resultsthe processes started without an additional dose of the drug for 118 patients ( 99.1% ) in group a and for 101 patients ( 84.8% ) in group b ( p=0.001 ) . 
 the average dose of ketamine administered to the patients in group a was 1.030.15  mg / kg and the average dose of propofol administered to the patients in group b was 1.460.55  mg / kg . none of the patients stopped the endoscopic procedure in group a , but one patient ( 0.8% ) had to discontinue the endoscopic procedure in group b. 27 patients in group a ( 22.7% ) and 41 patients ( 34.5% ) in group b developed complications during the procedure ( p=0.044 ) . 
 the rate of complications during the recovery of group a ( 110 patients , 92.4% ) was significantly higher than that in group b ( 48 patients , 40.3% ) ( p=0.001).conclusionin children , uge procedures can be quite comfortable when using the midazolam  ketamine combination . 
 however , adverse effects related to ketamine were observed during recovery .