assessment of right ventricular ( rv ) function is a challenge due to its complex and alternated morphology , especially in patients with congenital heart disease ( chd ) . regular and accurate assessment of rv function is an important part of the follow - up in such patients , since deterioration of rv function is closely related to mortality , and therefore , it guides therapy . as a rule of thumb , intervention , if possible , is considered , even in asymptomatic patients , when there are signs of worsening of rv function ( 1 , 2 ) . rv ejection fraction ( ef ) is a crude measurement of global rv systolic function , but as it correlates well with clinical endpoints ( 3 ) , it is very often used in clinical decision - making . cardiac magnetic resonance ( cmr ) imaging is well validated and has excellent correlation with true rv volumes ( 4 ) . it is considered the reference standard for assessment of the rv volumes and efs ( 5 ) but is expensive and not widely available . since echocardiography is more accessible than cmr , more patient - friendly , and less expensive , an echocardiographic alternative for assessment of rvef has been long sought for . the correlation between 2d echocardiographic measurements of rv function , current standard in clinical practice , and cmr - derived values of rvef is poor . real - time 3d echocardiographic assessment of rvef has proven to be reliable and feasible ( 6 ) , but its use in clinical practice is still limited . this is mostly due to the limited image quality , with inferior spatial and temporal resolution compared to 2d echocardiography , and also due to the substantial learning curve . a new alternative technique enabling 3d reconstruction of the rv is  knowledge - based 3d reconstruction ( 3d - kbr ) . kbr uses standardized 2d cross - sections of the rv combined with information about their localization in the 3d space to produced 3d reconstruction of rv volume ( 7 ) . kbr has shown to be accurate compared to cmr in children with various congenital heart anomalies ( 8) and in adults with the rv in systemic position and in pulmonary arterial hypertension ( 9 , 10 ) . the aim of the present study is to assess whether this kbr of rv is accurate and clinically feasible in the everyday practice of an echo - lab for adult chd ( achd ) of a tertiary referral centre . adult patients scheduled for routine cmr including assessment of rv volumes and ef , were asked to undergo an echo for kbr of the rv . cmr and echo were performed within 2  h from each other , to ensure comparable loading conditions . exclusion criteria were presence of cardiac devices , arrhythmia interfering with image acquisition , and inability to cooperate . as kbr uses diagnosis - specific algorithms for volume calculation , all patient 's data , including diagnosis , gender , weight , and height were put into the examination protocol before image storage . images for further kbr were acquired during end - expiratory breath holds in each of the various , predefined 2d cross - sections . the standard views and points of interest in all cross - section planes are shown in table 1 . rv , right ventricle ; rvot , rv outflow tract ; pa , pulmonary artery ; pv , pulmonary valve . echo examination was performed using the toshiba artida ssh-880cv diagnostic ultrasound system with a 5-mhz transducer . the ultrasound scanner was linked to a computer ( ventripoint medical systems ; ventripoint , inc . , seattle , wa , usa ) and a magnetic field receiver was attached to the ultrasound probe . the magnetic field transmitter generating the orthogonal magnetic field was located above the exam bed . the magnetic field data were recorded by the receiver at the time of image acquisition . using this data , the plane of the acquired 2d image was placed in the 3d volume created by the magnetic field transmitter . details of this system , technical specification , data acquisition and data analysis , are described elsewhere ( 7 , 11 ) . the acquired 2d rv - images and their spatial information were stored on the ventripoint medical systems computer attached to the ultrasound machine . to perform the image analysis , first end - diastole and end - systole were determined on each of the acquired clips . the definition of the end diastole was based on the ecg and visual assessment taking into account the largest rv cavity size and the opening and closure of tricuspid and pulmonary valves ( if possible ) . the same procedure was repeated to define the end systole , tracking the smallest rv cavity sizes . in the next step , 1535 points were placed in predefined crucial anatomical structures , as illustrated in table 1 . the procedure was done in both end - diastole and end - systole . combining data from these 2d cross - sections and their localization in the 3d space , the kbr system creates a 3d model of the rv using piecewise smooth subdivision surface reconstruction technology . the software compares acquired echo data with a ( cmr - derived ) reference database of patients with corresponding pathology ( patients with tetralogy of fallot with the cmr - derived data base for patients with tetralogy of fallot , etc . ) , allowing reconstruction and measurements of rv volumes ( fig . ( a ) the mesh with the colored dots represent rv end diastolic volume ( rvedv ) . demonstration of the position of the points on the mesh surface in relation to the calculated volume . red , rv endocardium ; light blue , rv septum ; pink , pulmonary annulus ; dark green , sub pulmonary ; violet , tricuspid annulus ; brown , basal bulge ; yellow , apex ; light green , rv septal edge . ( b ) combined edv and end systolic volume ( esv ) rendering , visualize the inward movement of different segments of the rv . after the first reconstruction , a quality check was performed using the incorporated features for quality assessment ( border delineation , scan plane , intersections , and adjustment of the placement of points ) . the corrected , reconstructed rv shape was further reviewed in each of the 2d images in both end diastole and end systole and optimized by deletion or addition of points . after performed control and carrying out modifications , the final reconstruction with calculated volumes was accepted ( fig . these values , both volumes and ef , calculated with kbr technology were compared with the cmr data of the same patient . subjects were scanned according to a pre - defined imaging protocol without anesthesia or sedation . a 1.5-t system ( ingenia r4.1.2 , philips healthcare , best , the netherlands ) was used with a dedicated chest phased - array parallel - imaging capable surface coil with a maximum of 36 active elements . steady state free precession cine images were acquired in various orientations ( short axis , four - chamber and two - chamber long axis , right and left ventricular outflow tract views in two planes ) during repeated end - expiratory breath holds . multi - slice cine short - axis acquisition was planned from the apex to well above the tricuspid and mitral valve : repetition time ( tr)/echo time ( te ) 3.4/1.69  ms , voxel size 1.251.258  mm , flip angle 90 , matrix 192171  mm , and 30 frames / cycle . rv volumetric analysis was performed by manual tracing of endocardial contours in end diastolic and end systolic phase in all slices , using philips cardiac explorer ( philips ews ( release 2.6 ) , philips medical systems , best , the netherlands ) . the end diastolic and end systolic phase was selected by visual assessment as the phase with the largest and smallest rv cavity sizes respectively , taking into account the longitudinal four - chamber , vertical two - chamber , and rv outflow tract as reference views . if visual assessment was difficult , multiple frames were contoured to determine the correct end diastolic or end systolic phase . the rv epicardial and endocardial contours were manually traced from the most apical to the most basal short - axis slice . only the portion of the outflow tract below the pulmonary valve was included in the blood volume in the slice where the valve was visible . if more than 50% of the tricuspid annulus or atrium was visible in a basal slice , the valve area was excluded from the blood volume . different statistical analyses were used to test the inter - technique correlation between parameters ( end diastolic volume ( edv ) , indexed edv ( edvi ) , end systolic volume ( esv ) , indexed esv ( esvi ) , and ef ) measured using two different methods . altman plot was constructed with the difference between measured values on y - axis and average of measured values on x - axis . ninety - five percent limits of agreements were computed to see how far apart the measurements by the two methods were likely to be . finally , an intraclass correlation ( icc ) coefficient was performed for all parameters . adult patients scheduled for routine cmr including assessment of rv volumes and ef , were asked to undergo an echo for kbr of the rv . cmr and echo were performed within 2  h from each other , to ensure comparable loading conditions . exclusion criteria were presence of cardiac devices , arrhythmia interfering with image acquisition , and inability to cooperate . patients were studied in the left lateral decubitus position . they were asked to lie absolutely still during the entire study . as kbr uses diagnosis - specific algorithms for volume calculation , all patient 's data , including diagnosis , gender , weight , and height were put into the examination protocol before image storage . images for further kbr were acquired during end - expiratory breath holds in each of the various , predefined 2d cross - sections . the standard views and points of interest in all cross - section planes rv , right ventricle ; rvot , rv outflow tract ; pa , pulmonary artery ; pv , pulmonary valve . echo examination was performed using the toshiba artida ssh-880cv diagnostic ultrasound system with a 5-mhz transducer . the ultrasound scanner was linked to a computer ( ventripoint medical systems ; ventripoint , inc . , seattle , wa , usa ) and a magnetic field receiver was attached to the ultrasound probe . the magnetic field transmitter generating the orthogonal magnetic field was located above the exam bed . the magnetic field data were recorded by the receiver at the time of image acquisition . using this data , the plane of the acquired 2d image was placed in the 3d volume created by the magnetic field transmitter . details of this system , technical specification , data acquisition and data analysis , are described elsewhere ( 7 , 11 ) . the acquired 2d rv - images and their spatial information were stored on the ventripoint medical systems computer attached to the ultrasound machine . to perform the image analysis , first end - diastole and end - systole were determined on each of the acquired clips . the definition of the end diastole was based on the ecg and visual assessment taking into account the largest rv cavity size and the opening and closure of tricuspid and pulmonary valves ( if possible ) . the same procedure was repeated to define the end systole , tracking the smallest rv cavity sizes . in the next step , 1535 points were placed in predefined crucial anatomical structures , as illustrated in table 1 . combining data from these 2d cross - sections and their localization in the 3d space , the kbr system creates a 3d model of the rv using piecewise smooth subdivision surface reconstruction technology . the software compares acquired echo data with a ( cmr - derived ) reference database of patients with corresponding pathology ( patients with tetralogy of fallot with the cmr - derived data base for patients with tetralogy of fallot , etc . ) , allowing reconstruction and measurements of rv volumes ( fig . ( a ) the mesh with the colored dots represent rv end diastolic volume ( rvedv ) . demonstration of the position of the points on the mesh surface in relation to the calculated volume . red , rv endocardium ; light blue , rv septum ; pink , pulmonary annulus ; dark green , sub pulmonary ; violet , tricuspid annulus ; brown , basal bulge ; yellow , apex ; light green , rv septal edge . ( b ) combined edv and end systolic volume ( esv ) rendering , visualize the inward movement of different segments of the rv . after the first reconstruction , a quality check was performed using the incorporated features for quality assessment ( border delineation , scan plane , intersections , and adjustment of the placement of points ) . the corrected , reconstructed rv shape was further reviewed in each of the 2d images in both end diastole and end systole and optimized by deletion or addition of points . after performed control and carrying out modifications , the final reconstruction with calculated volumes was accepted ( fig . these values , both volumes and ef , calculated with kbr technology were compared with the cmr data of the same patient . subjects were scanned according to a pre - defined imaging protocol without anesthesia or sedation . a 1.5-t system ( ingenia r4.1.2 , philips healthcare , best , the netherlands ) was used with a dedicated chest phased - array parallel - imaging capable surface coil with a maximum of 36 active elements . steady state free precession cine images were acquired in various orientations ( short axis , four - chamber and two - chamber long axis , right and left ventricular outflow tract views in two planes ) during repeated end - expiratory breath holds . multi - slice cine short - axis acquisition was planned from the apex to well above the tricuspid and mitral valve : repetition time ( tr)/echo time ( te ) 3.4/1.69  ms , voxel size 1.251.258  mm , flip angle 90 , matrix 192171  mm , and 30 frames / cycle . rv volumetric analysis was performed by manual tracing of endocardial contours in end diastolic and end systolic phase in all slices , using philips cardiac explorer ( philips ews ( release 2.6 ) , philips medical systems , best , the netherlands ) . the end diastolic and end systolic phase was selected by visual assessment as the phase with the largest and smallest rv cavity sizes respectively , taking into account the longitudinal four - chamber , vertical two - chamber , and rv outflow tract as reference views . if visual assessment was difficult , multiple frames were contoured to determine the correct end diastolic or end systolic phase . the rv epicardial and endocardial contours were manually traced from the most apical to the most basal short - axis slice . only the portion of the outflow tract below the pulmonary valve was included in the blood volume in the slice where the valve was visible . if more than 50% of the tricuspid annulus or atrium was visible in a basal slice , the valve area was excluded from the blood volume . different statistical analyses were used to test the inter - technique correlation between parameters ( end diastolic volume ( edv ) , indexed edv ( edvi ) , end systolic volume ( esv ) , indexed esv ( esvi ) , and ef ) measured using two different methods . altman plot was constructed with the difference between measured values on y - axis and average of measured values on x - axis . ninety - five percent limits of agreements were computed to see how far apart the measurements by the two methods were likely to be . nineteen consecutive , unselected patients ( 13 men and six women ) with chd were studied . the diagnosis was tetralogy of fallot with stenosis and/or insufficiency in pulmonary homograft ( ten patients ) , transposition of the great arteries in eight patients : three after atrial switch repair , four after arterial switch surgery , and one after rastelli correction . mean body surface area was 1.80.25  m and mean body weight 6814  kg . the 2d - kbr was possible in 16 out of 19 patients ( 85% ) . in two out of 19 patients , reconstruction was not possible due to difficulty in identifying the anatomical landmarks ( pulmonary valve and tricuspid annulus ) . in one patient , the kbr was not possible because the tracing did not lead to a sufficiently accurate reconstruction despite adjustments made during the quality check . results of the cmr and kbr analysis of the 16 individual patients were compared ( fig . 2 ) . when grouped , rv volumes assessed with kbr were smaller than cmr . comparison between knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) for right ventricle end systolic volumes ( rvesv ) and right ventricle end diastolic volumes ( rvedv ) . 11417  ml vs cmr edvi 12119  ml ( p<0.05 ) , esvi 6314  ml vs 6416  ml ( p value 0.5 ) and rvef 458% vs 479% respectively ( p<0.05 ) . the agreement between both methods assessed with icc was of 0.84 for rv edvi , 0.90 for rv esvi and 0.89 for rvef , p value < 0.001 . the difference in indexed rv edvi was 7  ml , with the limits of agreement ( 23.0 ; 8.7 ) , p=0.003 . difference for rvef was 2% with the limits of agreement ( 9.0 ; 5.0 ) , p=0.04 . a paired sample t - test was conducted to evaluate whether a statistically significant difference existed between the mean indexed rv volumes measured with the two modalities . the results of the paired sample t - test indicated no significant difference between indexed edv and ef calculated with both modalities , on the p level 0.05 ( table 2 ) . edv ( ml ) , end diastolic volume ; edvi ( ml / m ) , edv indexed ; esvi ( ml / m ) , end systolic volume indexed ; sv ( ml ) , stroke volume ; ef% , ejection fraction measured with knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) respectively . the linear regression analysis of rvedvi , rvesvi , and ef , the bland  altman analysis and the percentage difference between both techniques ( the difference between paired measurements divided by the average of the two measurements times 100 ) are shown in fig . ( a ) intertechnique correlation between knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) for end diastolic volume indexed ( edvi ) ( 1 ) , end systolic volume indexed ( esvi ) ( 2 ) , and ejection fraction ( ef ) ( 3 ) . altman plots between kbr and cmr for edvi ( 1 ) , esvi ( 2 ) , and ef ( 3 ) . edvi_diff ( kbredvicmredvi ) ; edvi_mean ( kbredvi+cmredvi)/2 . esvi_diff ( kbrsevicmresvi ) ; esvi_mean ( kbresvi+cmresvi)/2 . ef_diff ( kbrefcmref ) ; ef_mean ( kbref+cmref)/2 . altman plots between kbr and cmr for edv ( 1 ) , esv ( 2 ) , and ef ( 3 ) . edvi_diff_proc ( ( kbredvicmredvi)/edv_mean100 ) . the 2d - kbr was possible in 16 out of 19 patients ( 85% ) . in two out of 19 patients , reconstruction was not possible due to difficulty in identifying the anatomical landmarks ( pulmonary valve and tricuspid annulus ) . in one patient , the kbr was not possible because the tracing did not lead to a sufficiently accurate reconstruction despite adjustments made during the quality check . results of the cmr and kbr analysis of the 16 individual patients were compared ( fig . 2 ) . when grouped , rv volumes assessed with kbr were smaller than cmr . comparison between knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) for right ventricle end systolic volumes ( rvesv ) and right ventricle end diastolic volumes ( rvedv ) . 11417  ml vs cmr edvi 12119  ml ( p<0.05 ) , esvi 6314  ml vs 6416  ml ( p value 0.5 ) and rvef 458% vs 479% respectively ( p<0.05 ) . the agreement between both methods assessed with icc was of 0.84 for rv edvi , 0.90 for rv esvi and 0.89 for rvef , p value < 0.001 . the difference in indexed rv edvi was 7  ml , with the limits of agreement ( 23.0 ; 8.7 ) , p=0.003 . difference for rvef was 2% with the limits of agreement ( 9.0 ; 5.0 ) , p=0.04 . a paired sample t - test was conducted to evaluate whether a statistically significant difference existed between the mean indexed rv volumes measured with the two modalities . the results of the paired sample t - test indicated no significant difference between indexed edv and ef calculated with both modalities , on the p level 0.05 ( table 2 ) . edv ( ml ) , end diastolic volume ; edvi ( ml / m ) , edv indexed ; esvi ( ml / m ) , end systolic volume indexed ; sv ( ml ) , stroke volume ; ef% , ejection fraction measured with knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) respectively . altman analysis and the percentage difference between both techniques ( the difference between paired measurements divided by the average of the two measurements times 100 ) are shown in fig . ( a ) intertechnique correlation between knowledge - based reconstruction ( kbr ) and cardiac magnetic resonance ( cmr ) for end diastolic volume indexed ( edvi ) ( 1 ) , end systolic volume indexed ( esvi ) ( 2 ) , and ejection fraction ( ef ) ( 3 ) . altman plots between kbr and cmr for edvi ( 1 ) , esvi ( 2 ) , and ef ( 3 ) . edvi_diff ( kbredvicmredvi ) ; edvi_mean ( kbredvi+cmredvi)/2 . altman plots between kbr and cmr for edv ( 1 ) , esv ( 2 ) , and ef ( 3 ) . edvi_diff_proc ( ( kbredvicmredvi)/edv_mean100 ) . this study shows that also in adult patients with congenital heart defects , kbr produces rv volumes and ef that are sufficiently parallel to those derived from cmr to be clinically useful in a  real - world setting . despite a 2d image quality that is generally inferior to that in children , it is good enough to allow kbr to produce reliable 3d reconstructions of rv volumes . although the bland  altman test showed a systematic underestimation of echo derived rv volumes when compared with cmr , the differences were small . apart from one study in which there is an over - estimation of rv volumes measured with echo , compared with cmr ( 9 ) , our data are consistent with that of virtually all other studies on correlation between 3d echo and cmr for either left ventricular or rvef ( 4 , 12 , 13 ) . the underestimation of volumes with kbr is systematic and quantitatively limited and this should not hamper the use of kbr in clinical practice , provided echo - derived data are only compared to previously obtained echo - derived data . in contrast to conventional 2d echo , which is hardly of use for rv volume assessment in the individual patient in clinical practice ( 13 , 14 ) , the observed icc of around 0.9 between kbr- and cmr - derived rvedvi and rvesvi means that kbr can be used in clinical practice for assessment of rv volumes and function ( table 3 ) . it is a very important aspect in the follow - up of adults with chd , e.g. , in timing of pulmonary valve replacement in patients with tetralogy of fallot ( 15 , 16 ) . the fact that these volumes may now be assessed reliably with kbr with limited investment of time and money ( validated in the study by laser et al . cmr will still provide an additional information , prior to surgery , such as anatomic details , but its exploitation could probably be limited . in that way cmr resources would be more efficiently used in situations when we do not have other diagnostic tools . rv , right ventricle ; edv , end diastolic volume ; edvi , edv indexed ; esvi , end systolic volume indexed ; ef , ejection fraction ; sv , stroke volume ; icc , intraclass correlation . * p value of paired student 's t - test and 
 p value of icc . the limits of agreement for ef calculated with kbr compared with that calculated with cmr are quite wide : 2.411.4% . this should be taken into account when using kbr - derived ef in clinical practice . however , it should be realized that this is also true for cmr : when cmr is compared with itself , in repeated measurements of ef by test retest ( 17 ) or inter- or intra - observer variability ( 18 , 19 , 20 , 21 ) the biass.d . are of a comparable degree . the awareness about those limitations causes the decision about surgery to be based on repeated examinations in combination with patients ' exercise capacities and symptoms . the kbr system has the advantage of using 2d cross - sections as a basis for the 3d reconstruction . 2d echo has substantially higher spatial and temporal resolution than the rt3d echo images , acquired with the currently available 3d echo probes . the higher resolution makes identification of the endocardial border more straightforward and the anatomical structures that have to be defined are easier to recognize in the sharper 2d images than in the fuzzier rt3d images . a disadvantage is that kbr can only be used for volumetry of the rv ( currently ) , not for the lv , and no analysis of anatomic structures like valves or abnormal anatomy is possible . in contrast to cmr , the kbr system is a bedside technique , which can be used in various clinical settings , including icu . it is less expensive than cmr , faster in terms of data acquisition , with that more patient friendly , and data analysis ( 12 ) . the learning curve for both cmr and kbr is comparable and quite long but time consumption per patient is relatively short for kbr . in experienced hands , time required for examination , image analysis , and post processing with kbr is about 20  min  given good internet connection with the central data base . this corresponds to time needed for performing a cmr examination , but then time for data post processing and analysis must be added . this statement is based on the authors ' own experiences with kbr and cmr and on the previously cited paper by laser et al . because time consumption per examination is not systematically evaluated in the current study , the data are not reported in the part concerning results . cutting cost and increasing effectiveness , regarding both staff and equipment , is an important issue in the healthcare all around europe and in the whole world . the studied population is rather small and further investigation in larger and varying population , regarding both pathology and age , is recommended before the method can be the part of routine follow - up in achd population . we excluded one patient with rastelli corrected transposition due to poor image quality and inaccurate reconstructions . very complicated morphologies and rare corrections are difficult to assess , as it is not possible to visualize the standardized cross - sections for detection of points of interest for further reconstruction . however , this not only the kbr problem , assessment of the rv function in those patients is very challenging with all modalities . kbr system requires a specific equipment , such as magnetic field generator and the specialized bed compatible with the magnetic field . modification of the system in order to make it more customer friendly and to decrease time necessary for preparation , will probably be done parallel with the method 's further development . another limitation of presented study is the lack of data about inter and intra observer variability . in that aspect both studies show a good reproducibility of the rv volume calculations with the limits of agreements being marginally wider than for cmr measurements , both for intra- and inter - observer variability . test retest and intra- and inter - observer variability should be tested more profoundly , in large patient populations with various malformations before this technique can really be used with confidence in clinical practice . the experience in kbr is still relatively limited ; future studies in larger patients ' population with a greater variety of rv pathologies and an increased number of experienced investigators will probably give more information and more implications on how this new technology can be used in clinics . in our experience , kbr enables reliable measurement of rv volumes in an achd population with sufficiently high feasibility and accuracy even when used in an unselected patient population . rv volumes and ef are comparable to that of cmr , acknowledging a small systemic underestimation . in contrast to 2d echo , kbr of the rv can be used in daily clinical practice for analysis of rv volumes and ef . the authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported . this research did not receive any specific grant from any funding agency in the public , commercial or not - for - profit sector .
aimassessment of right ventricular ( rv ) function is a challenge , especially in patients with congenital heart disease ( chd ) . the aim of the present study is to assess whether knowledge - based rv reconstruction , used in the everyday practice of an echo - lab for adult chd in a tertiary referral center , is accurate when compared to cardiac magnetic resonance ( cmr ) examination.subjects and methodsadult patients who would undergo cmr for assessment of the rv were asked to undergo an echo of the heart for further knowledge - based reconstruction ( kbr ) . 
 echocardiographic images were acquired in standard views using a predefined imaging protocol . 
 rv volumes and ejection fraction ( ef ) calculated using knowledge - based technology were compared with the cmr data of the same patient.resultsnineteen consecutive patients with congenital right heart disease were studied . 
 median age of the patients was 28 years ( range 46 years ) . 
 reconstruction was possible in 16 out of 19 patients ( 85% ) . 
 rv volumes assessed with this new method were smaller than with cmr . 
 indexed end diastolic volumes were 11417  ml vs 12119  ml , p<0.05 and efs were 458% vs 479% , p<0.05 respectively . 
 the correlation between the methods was good with an intraclass correlation of 0.84 for edv and 0.89 for ef , p value < 0.001 in both cases.conclusionkbr enables reliable measurement of rvs in patients with chds and can be used in clinical practice for analysis of volumes and efs .