tumors that contain this translocation have been associated with an oligodendroglial phenotype , a better prognosis , and a better response to postoperative treatment , though the biological role of this marker remains unclear . in 2012 , follow - up results of more than 1112 years in the radiation therapy oncology group ( rtog ) 9402 and european organization for research and treatment of cancer ( eortc ) 26951 trials demonstrated that an overall survival benefit from the addition of chemotherapy to radiotherapy was confined to patients with anaplastic oligodendroglial tumors with ( versus without ) 1p/19q co - deletion , . the complete 1p/19q codeletion must be distinguished from partial 1p or 19q loss that , so far , lacks prognostic significance . evidence suggests that 1p/19q codeletion is homogeneous within a tumor and does not change during disease evolution . currently , two international randomized trials are investigating sequence and the combination of radiotherapy and chemotherapy in who grade iii tumors stratified according to 1p/19q status , . methylation of the mgmt gene promoter results in epigenetic silencing of the methyltransferase , which loses its gene repair activity . more than 15 years ago , reports indicated that high activity of the mgmt protein in glioma tissue was associated with resistance to alkylating agents , which , at that time , were largely nitrosoureas . in 2000 , methylation of the promoter region of the mgmt gene was linked to improved outcomes . in 2005 , mgmt promoter methylation assessed by a methylation - specific polymerase chain reaction was able to predict benefit from the addition of temozolomide ( tmz ) chemotherapy to radiotherapy in the treatment of newly diagnosed glioblastoma multiforme ( gbm ) . however , standardizing the mgmt assay for widespread clinical use was challenging , and treatment decisions continued to be performed without knowledge of the mgmt status . in 2012 , two randomized trials performed in the growing population of elderly gbm patients demonstrated consistently that a methylated mgmt promoter is a powerful predictive biomarker for benefit from tmz alone . in the german noa-08 trial , patients older than 65 years were treated with either standard 6-week , fractionated ( 1.82.0 gy ) radiotherapy or dose - dense tmz chemotherapy ( week on / week off ) . patients with tumors exhibiting methylated mgmt fared better if they were treated with tmz alone than those treated with radiotherapy alone . similarly , the nordic trial found standard - dose tmz ( 5 out of 28 days ) to be superior to radiotherapy in patients older than 60 years with methylated mgmt promoter . thus , at least in the elderly population , mgmt testing should become a standard procedure for decision making ( chemotherapy vs. radiotherapy ) , though the test is not yet widely available . whether patients with mgmt promoter methylation of other age groups or other who grades should be treated with tmz alone rather than chemoradiotherapy point mutations in the idh1 and idh2 genes , originally discovered in 2008 , occur in the vast majority of low - grade gliomas ( > 80% ) and secondary high - grade gliomas . the frequency of these mutations does not change during the progression from who grade ii to who grades iii or iv ( so - called secondary gbm ) . evidence has accumulated that primary and secondary gbm develop through different genetic pathways , though they remain largely histomorphologically indistinguishable at diagnosis . idh1/2 mutations , which occur early in gliomagenesis , change the function of the enzymes , causing them to produce 2-hydroxyglutarate , a possible oncometabolite , instead of -ketoglutarate . gliomas with a mutated idh1 or , less frequently , mutated idh2 are associated with better prognosis compared to their wild - type counterparts . as with loss of heterozygosity 1p/19q , a given idh status seems to be homogeneous within a tumor and does not change during disease evolution . mutated idh can easily be detected by immunohistochemistry and potentially even non - invasively by magnetic resonance spectroscopy . non - tumoral glial cells ( i.e. those involved in gliosis ) never express mutated idh , a fact that can be used to separate reactive gliosis from gliomas . pilocytic astrocytoma ( who grade i ) , ependymoma , and primary gbm ( but not secondary gbm ) do not harbor idh mutations . of note , furthermore , there is currently no drug that targets mutated idh , although this remains an area of active research . a tumor - specific mutant of the egfr , efgr variant iii ( egfrviii ) , causes constitutive activation of the receptor 's tyrosine kinase activity and is frequently expressed in primary gbm ( 33% ) . because it is localized solely on tumor tissue , egfrviii presents an ideal target for immunotherapy , reducing the risk of autoimmune toxicity . immunohistochemical testing for egfrviii may be implemented if randomized trials demonstrate activity of egfrviii - targeted vaccination , . among low - grade and anaplastic gliomas , nearly all with however , some genetic markers , such as egfr and idh1 , egfr and tp53 , tp53 and 1p/19q , are mutually exclusive . molecularly , idh1 and idh2 mutations are heterozygous , affect only a single codon , and rarely occur together . although tp53 mutations and 1p/19q codeletions are mutually exclusive , idh1 mutations are common in both of these genotypes , . tumors that contain this translocation have been associated with an oligodendroglial phenotype , a better prognosis , and a better response to postoperative treatment , though the biological role of this marker remains unclear . in 2012 , follow - up results of more than 1112 years in the radiation therapy oncology group ( rtog ) 9402 and european organization for research and treatment of cancer ( eortc ) 26951 trials demonstrated that an overall survival benefit from the addition of chemotherapy to radiotherapy was confined to patients with anaplastic oligodendroglial tumors with ( versus without ) 1p/19q co - deletion , . the complete 1p/19q codeletion must be distinguished from partial 1p or 19q loss that , so far , lacks prognostic significance . evidence suggests that 1p/19q codeletion is homogeneous within a tumor and does not change during disease evolution . currently , two international randomized trials are investigating sequence and the combination of radiotherapy and chemotherapy in who grade iii tumors stratified according to 1p/19q status , . methylation of the mgmt gene promoter results in epigenetic silencing of the methyltransferase , which loses its gene repair activity . more than 15 years ago , reports indicated that high activity of the mgmt protein in glioma tissue was associated with resistance to alkylating agents , which , at that time , were largely nitrosoureas . in 2000 , methylation of the promoter region of the mgmt gene was linked to improved outcomes . in 2005 , mgmt promoter methylation assessed by a methylation - specific polymerase chain reaction was able to predict benefit from the addition of temozolomide ( tmz ) chemotherapy to radiotherapy in the treatment of newly diagnosed glioblastoma multiforme ( gbm ) . however , standardizing the mgmt assay for widespread clinical use was challenging , and treatment decisions continued to be performed without knowledge of the mgmt status . in 2012 , two randomized trials performed in the growing population of elderly gbm patients demonstrated consistently that a methylated mgmt promoter is a powerful predictive biomarker for benefit from tmz alone . in the german noa-08 trial , patients older than 65 years were treated with either standard 6-week , fractionated ( 1.82.0 gy ) radiotherapy or dose - dense tmz chemotherapy ( week on / week off ) . patients with tumors exhibiting methylated mgmt fared better if they were treated with tmz alone than those treated with radiotherapy alone . similarly , the nordic trial found standard - dose tmz ( 5 out of 28 days ) to be superior to radiotherapy in patients older than 60 years with methylated mgmt promoter . thus , at least in the elderly population , mgmt testing should become a standard procedure for decision making ( chemotherapy vs. radiotherapy ) , though the test is not yet widely available . whether patients with mgmt promoter methylation of other age groups or other who grades should be treated with tmz alone rather than chemoradiotherapy point mutations in the idh1 and idh2 genes , originally discovered in 2008 , occur in the vast majority of low - grade gliomas ( > 80% ) and secondary high - grade gliomas . the frequency of these mutations does not change during the progression from who grade ii to who grades iii or iv ( so - called secondary gbm ) . evidence has accumulated that primary and secondary gbm develop through different genetic pathways , though they remain largely histomorphologically indistinguishable at diagnosis . idh1/2 mutations , which occur early in gliomagenesis , change the function of the enzymes , causing them to produce 2-hydroxyglutarate , a possible oncometabolite , instead of -ketoglutarate . gliomas with a mutated idh1 or , less frequently , mutated idh2 are associated with better prognosis compared to their wild - type counterparts . as with loss of heterozygosity 1p/19q , a given idh status seems to be homogeneous within a tumor and does not change during disease evolution . mutated idh can easily be detected by immunohistochemistry and potentially even non - invasively by magnetic resonance spectroscopy . non - tumoral glial cells ( i.e. those involved in gliosis ) never express mutated idh , a fact that can be used to separate reactive gliosis from gliomas . pilocytic astrocytoma ( who grade i ) , ependymoma , and primary gbm ( but not secondary gbm ) do not harbor idh mutations . of note , furthermore , there is currently no drug that targets mutated idh , although this remains an area of active research . a tumor - specific mutant of the egfr , efgr variant iii ( egfrviii ) , causes constitutive activation of the receptor 's tyrosine kinase activity and is frequently expressed in primary gbm ( 33% ) . because it is localized solely on tumor tissue , egfrviii presents an ideal target for immunotherapy , reducing the risk of autoimmune toxicity . immunohistochemical testing for egfrviii may be implemented if randomized trials demonstrate activity of egfrviii - targeted vaccination , . among low - grade and anaplastic gliomas , nearly all with 1p/19q codeletion also harbor idh1/2 mutations . however , some genetic markers , such as egfr and idh1 , egfr and tp53 , tp53 and 1p/19q , are mutually exclusive . molecularly , idh1 and idh2 mutations are heterozygous , affect only a single codon , and rarely occur together . although tp53 mutations and 1p/19q codeletions are mutually exclusive , idh1 mutations are common in both of these genotypes , . the most recent clinical data from randomized phase iii trials call for routine testing of 1p/19q for patients with who grade iii gliomas and for assessing the mgmt methylation status , especially in elderly gbm patients too frail to undergo postoperative concomitant radiochemotherapy followed by chemotherapy  the standard treatment for gbm . molecular marker determination , however , is technically demanding and requires reproducible and validated test procedures . this holds especially true for mgmt testing , where results sometimes may fall into a  gray zone . braf mutations have been found in a fraction of high - grade glioma patients ( e.g. epitheloid gbm ) and may present a druggable treatment target for specific inhibitors such as vemurafenib or dabrafenib , . another focus of interest is immunosuppressive molecules ( e.g. , b7h1 and b7h4 ) ; further research is warranted to define the role of immunomodulatory drugs in high - grade glioma . moreover , we still lack biomarkers with predictive properties to select anti - angiogenic agents for treating gliomas . circulating microrna ( mirna ) , small non - coding regulatory rnas that modulate the expression of specific target genes , might be relevant in the future for diagnosis , prognosis , and therapy of gliomas . for example , evaluating the circulating dna of egfrviii in plasma may represent a strategy to screen patients for an anti - egfrviii therapy and monitor response to treatment . the availability of high - throughput methods will most likely enrich the histomorphological who classification with a comprehensive molecular characterization of gliomas .
the current world health organization classification system of primary brain tumors is solely based on morphologic criteria . 
 however , there is accumulating evidence that tumors with similar histology have distinct molecular signatures that significantly impact treatment response and survival . 
 recent practice - changing clinical trials have defined a role for routine assessment of o-6-methylguanine - dna methyltransferase ( mgmt ) promoter methylation in glioblastoma patients , especially in the elderly , and 1p and 19q codeletions in patients with anaplastic glial tumors . 
 recently discovered molecular alterations including mutations in idh-1/2 , epidermal growth factor receptor ( egfr ) , and braf also have the potential to become targets for future drug development . 
 this article aims to summarize current knowledge on the molecular biology of high - grade gliomas relevant to daily practice .