tropicale , strain v5sourcecabo rojo , puerto ricogenome size , mb3.57gc content , % 67.6trna52rrna4protein coding sequences3246 the genus halorubrum belongs to the halophilic archaea and it currently comprises 31 species . members of this genus have been isolated from different hypersaline environments around the world including salterns , soda lakes , saline soil , and fermented foods , , , . the solar salterns of cabo rojo in puerto rico has been subjected to microbial studies over the years where several new species had been isolated and described , . a recent microbial survey of this environment isolated a novel strain ( v5 ) using pyruvate as the sole carbon and energy source . analysis using the 16s rrna gene revealed that this strain belongs to the genus halorubrum . phylogenetic analyses using the rpob , ppsa and atpb genes by multilocus sequence analysis and other physiological properties revealed that strain v5 represents a new species within this genus . the name h. tropicale was selected for this strain and a formal taxonomic description is in progress . the draft genome was 3,572,834  bp in length with g  +  c content of 67.6% . the genome was predicted to include 3405 open reading frames ( orfs ) , 3246 of which are protein - coding sequences , 4 rrna ( 5s ( 2 ) , 16s ( 1 ) , 23s ( 1 ) ) , and 52 trna genes . 1 ) ( http://rast.nmpdr.org/ ) , 1666 predicted genes were classified into subsystems , with 229 genes classified into the carbohydrate transport and metabolism subsystem . carbohydrate - active enzyme analysis performed by dbcan ( http://csbl.bmb.uga.edu/dbcan/ ) showed that most of these enzymes belonged to the glycoside hydrolase group , with 19 total enzymes in 10 different families . strain v5 was grown in solid media containing pond water from the sampling site which was diluted to 20% nacl ( w / v ) . the media also contained ( in g / l ) , yeast extract 5 , sodium pyruvate 5 , and agar 20 . the dna sample was sequenced at the molecular research lab ( mr dna ) facility in shallowater , tx , usa . the nextera dna sample preparation kit ( illumina ) was used following the manufacturer 's instructions to prepare the genomic library . the initial dna concentration was determined using the qubit dsdna hs assay kit ( life technologies ) . to achieve the recommended dna input of 50  ng , samples were diluted accordingly to a concentration of 2.5  ng/l . then samples underwent fragmentation and the addition of adapter sequences . these adapters are utilized during a limited - cycle ( 5  cycles ) pcr in which unique index was added to the sample . after the library was prepared , the final concentration was measured with the qubit dsdna hs assay kit ( life technologies ) , and the agilent 2100 bioanalyzer ( agilent technologies ) was utilized to determine the average library size . the library was then pooled in equimolar ratios of 2  nm , and 12  pm of the library pool was sequenced paired end for 600  cycles using the miseq system ( illumina ) . assembly was performed using the ngen assembler and resulted in 17 contigs ( n50  =  515,834  bp ) , the largest of which included 1,031,026  bp . protein coding sequences were predicted using rast ( rapid annotation subsystem technology ) , ribosomal rna genes were detected using rnammer 1.2 server   and trna genes were detected using aragorn . the authors declare that no conflict of interest exists about the work published in this paper .
the genus halorubrum is a member of the family halobacteriaceae which currently has the highest number of described species ( 31 ) of all the haloarchaea . here 
 we report the draft genome sequence of strain v5 , a new species within this genus that was isolated from the solar salterns of cabo rojo , puerto rico . 
 assembly was performed and rendered the genome into 17 contigs ( n50  =  515,834  bp ) , the largest of which contains 1,031,026  bp . 
 the genome consists of 3.57  mb in length with g  +  c content of 67.6% . in general , the genome includes 4 rrnas , 52 trnas , and 3246 protein - coding sequences . the ncbi accession number for this genome is list00000000 and the strain deposit number is cect9000 .