nonribosomal peptides ( nrps ) are of great pharmacological importance , but there is currently no technology for high - throughput nrp dereplication and sequencing . 
 we employ multi - stage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic nrps . 
 we also present an algorithm for comparative nrp dereplication that establishes similarities between newly isolated and previously identified similar but non - identical nrps , substantially reducing dereplication efforts ..