briefly , all latino women referred to los angeles county women 's hospital for management of gestational diabetes mellitus between august 1993 and march 1995 were asked to participate if they met all of the following criteria : 1 ) gestational age between 28 and 34 weeks , 2 ) no current or prior insulin therapy , 3 ) all fasting serum glucose concentrations < 130 mg / dl ( 7.2 mmol / l ) during pregnancy , 4 ) otherwise uncomplicated singleton pregnancy , and 5 ) both parents and at least three of four grandparents from mexico , guatemala , or el salvador . they were asked to return for a 75-g oral glucose tolerance test ( ogtt ) 6 months postpartum and then for an ogtt and an intravenous glucose tolerance test ( ivgtt ) at 15 months postpartum and every 15 months thereafter . height , weight , and information on contraceptive use and pregnancies were collected at each visit . bioelectrical impedance ( bia ) was measured at each ogtt visit to assess body composition . at the time of diagnosis of impaired glucose tolerance , our diabetic subjects met with a dietitian and received advice on nutrition and daily walking . subjects remained in follow - up until they withdrew consent , were lost to follow - up , or developed a fasting plasma glucose concentration > 140 mg / dl ( > 7.8 mmol / l ) , at which time they were referred for pharmacological treatment . women who were pregnant at the time of a scheduled battery of tests were studied at least 4 months after pregnancy and at least 1 month after completion of breastfeeding . family history of diabetes was not assessed for this cohort . for the present report , which is focused on nonpregnant women from 15 to 75 months postpartum ( occurred between october 1994 and november 2001 ) , we analyzed data from all subjects who 1 ) had a baseline ogtt and ivgtt without diabetes in the 15- or 30-month postpartum testing window and 2 ) returned for at least one additional set of ogtt and ivgtt by the 75-month postpartum test window . all subjects gave written informed consent for participation in the study , which was approved by the institutional review board of the university of southern california and the los angeles county plus university of southern california medical center . for a battery of ogtt , ivgtt , and bia , subjects came to the general clinical research center on 2 separate days , at least 48 h apart , after an 8- to 12-h overnight fast and at least 3 days on an unrestricted diet . on day 1 , bia was measured immediately before an ogtt between 0700 and 1000 . for bia , subjects lay supine while plastic electrodes were placed on their right hand and foot and a trained technician took dual resistance and reactance readings with a quantom impedance meter ( rjl systems , michigan ) . for ogtts , blood was obtained from an antecubital venous catheter before and 15 , 30 , 60 , 90 , and 120 min after the glucose ingestion and placed on ice , and plasma was separated within 20 min and stored at 80c . fasting plasma samples from the ogtts were used later to measure free fatty acids ( ffas ) , adiponectin , leptin , c - reactive protein ( crp ) , and interleukin ( il)-6 . on a separate day , an ivgtt was performed starting between 0700 and 1000 . dextrose ( 300 mg / kg ) was injected over 1 min , followed in 20 min by a 5-min infusion of crystalline human insulin ( 0.03 units / kg ) . arterialized venous blood was drawn into iced tubes before ( n = 2 ) and for 240 min after ( n = 32 ) the dextrose injection . glucose was measured by a glucose oxidase ( beckman glucose analyzer ii ; beckman coulter , brea , ca ) . insulin was measured by a radioimmunoassay ( ria ) ( novo pharmaceuticals , danbury , ct ) that measured insulin and proinsulin with intra- and inter - observer coefficient of variations ( cv ) of < 2.3 and 4.4% . plasma ffas were measured by an enzymatic colorimetric method using a kit from wako chemicals ( intra- and inter - observer cvs of < 0.75 and 0.37% ) . plasma adiponectin and leptin levels were measured using ria kits from linco research ( intra- and inter - observer cvs of < 8.3 and 9.2% ) . plasma crp and il-6 were measured using crp elisa ( intra- and inter - observer cvs of < 6.0 and 13.8% ) and ultrasensitive il-6 elisa kits ( intra- and inter - observer cvs of < 8.3 and 10.0% ) from alpco diagnostics . bmi was calculated as weight in kilograms divided by the square of height in meters . ivgtt results were analyzed using the minmod program ( 11 ) to obtain measures of fractional glucose disappearance due to an increase in insulin above basal ( insulin sensitivity [ si ] ) . the acute insulin response to intravenous glucose ( airg ) was calculated by the trapezoid rule as the incremental area under the insulin curve during the first 10 min after the glucose injection . the product of si and airg ( the disposition index [ di ] ) was calculated as a measure of acute pancreatic -cell compensation for insulin resistance ( 11 ) . body fat and fat - free mass were calculated by the formula of kotler et al . baseline characteristics are presented as mean ( sd ) and ranges ( table 1 ) . for data analysis , log - transformation was applied for crp , fasting and 2-h insulin , 30-min incremental insulin , si , airg , and di . rates of change for variables that were measured at each follow - up visit ( weight , body composition , airg , si , and di ) were estimated by regressing follow - up data in each subject against follow - up time for that subject . fasting ffa , adiponectin , crp , leptin , and il-6 were measured only at baseline and last follow - up visit , so their rates of change were estimated as [ last baseline]/[follow - up time ] for each subject . fasting total , hdl , and ldl cholesterol and triglycerides were not collected during follow - up . relationship between follow - up changes in glucose and di decline has been evaluated previously ( 4 ) and was not the focus in this report ; thus , follow - up changes on ogtt glucose and insulin were not included in this report . random coefficient mixed - effect models were used to test for significant changes over time for di , si , airg , weight , and body composition . mixed - effect models were also used to test for significant associations between changes in the primary outcome variable , di , and 1 ) baseline variables ( table 1 ) and 2 ) changes during follow - up in pregnancy status , use of hormonal contraception , body weight , fat and fat - free mass , circulating levels of ffa , adiponectin , crp , leptin and il-6 . in evaluating the prediction of baseline si , airg , and di on the primary outcome rate of change in di , we applied a bias correction to adjust for possible artificial negative correlation due to measurement error , sometimes referred to as  regression to the mean . measurement error accounted for 15 , 12 , and 15% of the total variation for si , airg , and di , respectively ( estimated from 109 hispanic women with similar characteristics who participated in a separate study where ivgtts were repeated after 3 months without any treatment ) . the bias corrected point estimates were obtained using an empirical formula developed through simulation for longitudinal repeated data ( 14 ) . standard errors of the estimates were obtained by the bootstrap method with 100 replications . in each bootstrap sample , 60 subjects were randomly selected with replacement from the original sample ; mixed - effect models were then fitted and bias corrected estimates were obtained . baseline and follow - up characteristics * geometric means  sds were presented for baseline crp , fasting and 2-h insulin , insulin at 30 min , insulin sensitivity , acute insulin response , and di . incremental insulin area during first 10 min of ivgtt . # a measure of -cell compensation for insulin resistance . significant changes : p < 0.001 . to convert the glucose in units of mg / dl to the si unit of mmol / l , multiply the number in the table by 0.0555 . all statistical tests were two - sided , and statistical significance was defined as p  0.05 . for a battery of ogtt , ivgtt , and bia , subjects came to the general clinical research center on 2 separate days , at least 48 h apart , after an 8- to 12-h overnight fast and at least 3 days on an unrestricted diet . on day 1 , bia was measured immediately before an ogtt between 0700 and 1000 . for bia , subjects lay supine while plastic electrodes were placed on their right hand and foot and a trained technician took dual resistance and reactance readings with a quantom impedance meter ( rjl systems , michigan ) . for ogtts , subjects drank 75 g dextrose . blood was obtained from an antecubital venous catheter before and 15 , 30 , 60 , 90 , and 120 min after the glucose ingestion and placed on ice , and plasma was separated within 20 min and stored at 80c . fasting plasma samples from the ogtts were used later to measure free fatty acids ( ffas ) , adiponectin , leptin , c - reactive protein ( crp ) , and interleukin ( il)-6 . on a separate day , an ivgtt was performed starting between 0700 and 1000 . dextrose ( 300 mg / kg ) was injected over 1 min , followed in 20 min by a 5-min infusion of crystalline human insulin ( 0.03 units / kg ) . arterialized venous blood was drawn into iced tubes before ( n = 2 ) and for 240 min after ( n = 32 ) the dextrose injection . glucose was measured by a glucose oxidase ( beckman glucose analyzer ii ; beckman coulter , brea , ca ) . insulin was measured by a radioimmunoassay ( ria ) ( novo pharmaceuticals , danbury , ct ) that measured insulin and proinsulin with intra- and inter - observer coefficient of variations ( cv ) of < 2.3 and 4.4% . plasma ffas were measured by an enzymatic colorimetric method using a kit from wako chemicals ( intra- and inter - observer cvs of < 0.75 and 0.37% ) . plasma adiponectin and leptin levels were measured using ria kits from linco research ( intra- and inter - observer cvs of < 8.3 and 9.2% ) . plasma crp and il-6 were measured using crp elisa ( intra- and inter - observer cvs of < 6.0 and 13.8% ) and ultrasensitive il-6 elisa kits ( intra- and inter - observer cvs of < 8.3 and 10.0% ) from alpco diagnostics . bmi was calculated as weight in kilograms divided by the square of height in meters . ivgtt results were analyzed using the minmod program ( 11 ) to obtain measures of fractional glucose disappearance due to an increase in insulin above basal ( insulin sensitivity [ si ] ) . the acute insulin response to intravenous glucose ( airg ) was calculated by the trapezoid rule as the incremental area under the insulin curve during the first 10 min after the glucose injection . the product of si and airg ( the disposition index [ di ] ) was calculated as a measure of acute pancreatic -cell compensation for insulin resistance ( 11 ) . body fat and fat - free mass were calculated by the formula of kotler et al . baseline characteristics are presented as mean ( sd ) and ranges ( table 1 ) . for data analysis , log - transformation was applied for crp , fasting and 2-h insulin , 30-min incremental insulin , si , airg , and di . thus , geometric means are presented for these variables where appropriate . rates of change for variables that were measured at each follow - up visit ( weight , body composition , airg , si , and di ) were estimated by regressing follow - up data in each subject against follow - up time for that subject . fasting ffa , adiponectin , crp , leptin , and il-6 were measured only at baseline and last follow - up visit , so their rates of change were estimated as [ last baseline]/[follow - up time ] for each subject . fasting total , hdl , and ldl cholesterol and triglycerides were not collected during follow - up . relationship between follow - up changes in glucose and di decline has been evaluated previously ( 4 ) and was not the focus in this report ; thus , follow - up changes on ogtt glucose and insulin were not included in this report . random coefficient mixed - effect models were used to test for significant changes over time for di , si , airg , weight , and body composition . mixed - effect models were also used to test for significant associations between changes in the primary outcome variable , di , and 1 ) baseline variables ( table 1 ) and 2 ) changes during follow - up in pregnancy status , use of hormonal contraception , body weight , fat and fat - free mass , circulating levels of ffa , adiponectin , crp , leptin and il-6 . in evaluating the prediction of baseline si , airg , and di on the primary outcome rate of change in di , we applied a bias correction to adjust for possible artificial negative correlation due to measurement error , sometimes referred to as  regression to the mean . measurement error accounted for 15 , 12 , and 15% of the total variation for si , airg , and di , respectively ( estimated from 109 hispanic women with similar characteristics who participated in a separate study where ivgtts were repeated after 3 months without any treatment ) . the bias corrected point estimates were obtained using an empirical formula developed through simulation for longitudinal repeated data ( 14 ) . standard errors of the estimates were obtained by the bootstrap method with 100 replications . in each bootstrap sample , 60 subjects were randomly selected with replacement from the original sample ; mixed - effect models were then fitted and bias corrected estimates were obtained . baseline and follow - up characteristics * geometric means  sds were presented for baseline crp , fasting and 2-h insulin , insulin at 30 min , insulin sensitivity , acute insulin response , and di . incremental insulin area during first 10 min of ivgtt . # a measure of -cell compensation for insulin resistance . significant changes : p < 0.001 . to convert the glucose in units of mg / dl to the si unit of mmol / l , all statistical tests were two - sided , and statistical significance was defined as p  0.05 . sixty women met the inclusion criteria for this report ; 28 had normal glucose tolerance and 32 had impaired glucose tolerance . at baseline ( table 1 ) , the cohort had an average age of 32 years and an average bmi and body fat consistent with obesity . mean glucose levels were consistent with impaired glucose tolerance , and mean insulin sensitivity was very low . there were 25 women who had all five possible visits , 19 had four , 12 had three , and 4 had two . during follow - up , 14 of the women developed diabetes , 15% of the women used estrogen - progestin combination oral contraceptives , and 15% used progestin - only contraceptives ; two women ( 3% ) used both at different times . a total of 25% of the women experienced one or more additional pregnancies during the follow - up period . during follow - up ( table 1 ) , mean weight increased significantly ( 0.75  1.18 kg / year ; p < 0.0001 ) , although some women gained and some lost weight . body fat also increased significantly ( 0.56  1.01 kg / year ; p < 0.0001 ) , and changes in body fat accounted for 75% of the changes in body weight . fasting ffa and leptin increased significantly ( p < 0.001 ) and adiponectin decreased significantly ( p < 0.01 ) . there were no significant changes across the cohort in crp , il-6 , or si . airg and di both fell significantly ( p < 0.001 ) . figure 1 depicts mean values for weight , di , si , and airg at each follow - up time . plot of mean weight , di , si , and first - phase insulin secretion ( airg ) by visit month . geometric means are shown for di , si , and airg ; arithmetic mean is shown for weight . univariate analysis with random coefficients mixed - effect modeling revealed that none of the baseline characteristics listed in table 1 except baseline airg and di were significantly associated with rate of change in di during follow - up ( p > 0.15 for each ) . baseline airg and di were significantly negatively associated with rate of change in di ( regression coefficient  se = 0.031  0.0107 log di unit per year / log airg unit , p = 0.004 , for baseline airg ; 0.0337  0.0103 log di unit per year / log di unit for baseline di , p = 0.0014 , respectively ) . after correcting for potential bias due to regression to the mean , baseline airg and di were no longer significantly associated with rate of change in di ( regression coefficients were reduced to 0.0056  0.0123 log di unit per year / log airg unit , p = 0.65 , for baseline airg ; 0.0009  0.0096 log di unit per year / log di unit for baseline di , p = 0.92 , respectively ) . for follow - up characteristics , additional pregnancies , use of hormonal contraception , changes in ffas , leptin , and il-6 were not significantly associated with rate of change in di ( p > 0.30 ) . rate of change in di was significantly associated with rates of change in weight ( 0.026  0.007 log di unit / kg , p = 0.0003 ) , adiponectin ( 0.055 + 0.018 log di unit per ng / ml  10 , p = 0.002 ) , and crp ( 0.110  0.035 log di unit per log ng / ml , p = 0.002 ) univariately . the association with crp was noted despite the fact that there was on average no significant change in crp in this cohort ( table 1 ) . results of the multivariate analysis with which we assessed the potential contributions of changing adiponectin and crp to the association between change in di and weight gain appear in table 2 . the univariate association between weight gain and declining di was reduced by 19% after adjustment for changes in either adiponectin or crp and by 31% after adjustment for both ( table 2 ) . nonetheless , change in weight remained significantly associated with change in di after these adjustments ( table 2 , adjusted p  0.02 ) . thus , weight gain was the strongest factor that associated with declining di , and that association was reduced but remained significant after adjustment for changes in adiponectin and crp . impact of changes in adiponectin and crp on the relationship between changes in weight and di during follow - up * estimated from random - coefficient mixed - effect model regression analysis . calculated as 100  ( unadjusted regression coefficient  adjusted regression coefficient)/unadjusted regression coefficient . we have previously shown that insulin resistance can contribute to declining -cell function in hispanic women ( 13,15 ) . to determine whether the pattern of association we observed between di , weight gain , and changing crp and adiponectin was simply due to insulin resistance , we performed analysis with follow - up si as a covariate . adjustment for changes in si alone reduced the association between weight gain and declining di by 40% , but the association remained statistically significant ( p = 0.037 ) . adding adjustment for changes in si to the previous 31% reduction caused by adjustment for adiponectin and crp resulted in a total of 70% reduction of the relationship between weight gain and declining di . indeed , the association between weight gain and declining di was no longer statistically significant ( p = 0.29 ) . the adjustment for changes in si had little impact on the relationship between failing di and changes in adiponectin and crp after weight gain was included . thus , changes in insulin resistance , adiponectin , and crp all appeared to contribute to the relationship between weight gain and declining di . multivariate analysis including the two major components of weight ( body fat and fat - free mass ) indicated that the decline in di was significantly associated with the gain in fat mass ( p = 0.01 ) , but not the fat - free mass ( p = 0.16 ) . sixty women met the inclusion criteria for this report ; 28 had normal glucose tolerance and 32 had impaired glucose tolerance . at baseline ( table 1 ) , the cohort had an average age of 32 years and an average bmi and body fat consistent with obesity . mean glucose levels were consistent with impaired glucose tolerance , and mean insulin sensitivity was very low . there were 25 women who had all five possible visits , 19 had four , 12 had three , and 4 had two . during follow - up , 14 of the women developed diabetes , 15% of the women used estrogen - progestin combination oral contraceptives , and 15% used progestin - only contraceptives ; two women ( 3% ) used both at different times . a total of 25% of the women experienced one or more additional pregnancies during the follow - up period . during follow - up ( table 1 ) , mean weight increased significantly ( 0.75  1.18 kg / year ; p < 0.0001 ) , although some women gained and some lost weight . body fat also increased significantly ( 0.56  1.01 kg / year ; p < 0.0001 ) , and changes in body fat accounted for 75% of the changes in body weight . fasting ffa and leptin increased significantly ( p < 0.001 ) and adiponectin decreased significantly ( p < 0.01 ) . there were no significant changes across the cohort in crp , il-6 , or si . airg and di both fell significantly ( p < 0.001 ) . figure 1 depicts mean values for weight , di , si , and airg at each follow - up time . plot of mean weight , di , si , and first - phase insulin secretion ( airg ) by visit month . geometric means are shown for di , si , and airg ; arithmetic mean is shown for weight . univariate analysis with random coefficients mixed - effect modeling revealed that none of the baseline characteristics listed in table 1 except baseline airg and di were significantly associated with rate of change in di during follow - up ( p > 0.15 for each ) . baseline airg and di were significantly negatively associated with rate of change in di ( regression coefficient  se = 0.031  0.0107 log di unit per year / log airg unit , p = 0.004 , for baseline airg ; 0.0337  0.0103 log di unit per year / log di unit for baseline di , p = 0.0014 , respectively ) . after correcting for potential bias due to regression to the mean , baseline airg and di were no longer significantly associated with rate of change in di ( regression coefficients were reduced to 0.0056  0.0123 log di unit per year / log airg unit , p = 0.65 , for baseline airg ; 0.0009  0.0096 log di unit per year / log di unit for baseline di , p = 0.92 , respectively ) . for follow - up characteristics , additional pregnancies , use of hormonal contraception , changes in ffas , leptin , and il-6 were not significantly associated with rate of change in di ( p > 0.30 ) . rate of change in di was significantly associated with rates of change in weight ( 0.026  0.007 log di unit / kg , p = 0.0003 ) , adiponectin ( 0.055 + 0.018 log di unit per ng / ml  10 , p = 0.002 ) , and crp ( 0.110  0.035 log di unit per log ng / ml , p = 0.002 ) univariately . the association with crp was noted despite the fact that there was on average no significant change in crp in this cohort ( table 1 ) . results of the multivariate analysis with which we assessed the potential contributions of changing adiponectin and crp to the association between change in di and weight gain appear in table 2 . the univariate association between weight gain and declining di was reduced by 19% after adjustment for changes in either adiponectin or crp and by 31% after adjustment for both ( table 2 ) . nonetheless , change in weight remained significantly associated with change in di after these adjustments ( table 2 , adjusted p  0.02 ) . thus , weight gain was the strongest factor that associated with declining di , and that association was reduced but remained significant after adjustment for changes in adiponectin and crp . impact of changes in adiponectin and crp on the relationship between changes in weight and di during follow - up * estimated from random - coefficient mixed - effect model regression analysis . we have previously shown that insulin resistance can contribute to declining -cell function in hispanic women ( 13,15 ) . to determine whether the pattern of association we observed between di , weight gain , and changing crp and adiponectin was simply due to insulin resistance , we performed analysis with follow - up si as a covariate . adjustment for changes in si alone reduced the association between weight gain and declining di by 40% , but the association remained statistically significant ( p = 0.037 ) . adding adjustment for changes in si to the previous 31% reduction caused by adjustment for adiponectin and crp resulted in a total of 70% reduction of the relationship between weight gain and declining di indeed , the association between weight gain and declining di was no longer statistically significant ( p = 0.29 ) . the adjustment for changes in si had little impact on the relationship between failing di and changes in adiponectin and crp after weight gain was included . thus , changes in insulin resistance , adiponectin , and crp all appeared to contribute to the relationship between weight gain and declining di . multivariate analysis including the two major components of weight ( body fat and fat - free mass ) indicated that the decline in di was significantly associated with the gain in fat mass ( p = 0.01 ) , but not the fat - free mass ( p = 0.16 ) . in relatively young and nondiabetic hispanic women with prior gestational diabetes mellitus , we were unable to identify baseline characteristics that were predictive of declining -cell compensation for insulin resistance over the next 5 years . approximately one - third of the association between weight gain and declining -cell compensation was accounted for statistically by these two variables . these associations were not simply due to increasing insulin resistance ; adjustment for change in insulin sensitivity accounted for an additional 40% of the association between weight gain and declining -cell function . on average , 75% of weight gain was body fat , and it was gain in fat , not lean mass , that was associated with declining di . taken together , our findings indicate that weight gain was the strongest factor that contributed to the decline in -cell compensation for insulin resistance in hispanic women at high risk for type 2 diabetes . such an effect was mediated through at least two effects : increasing insulin resistance and alterations in adipokine levels . the significant association between declining -cell compensation and weight gain confirmed observations made previously by weyer et al . in pima indians ( 5 ) and by kriketos et al . ( 6 ) in women with a family history of type 2 diabetes . in weyer 's study , 209 nondiabetic pima indians ( 151 with normal glucose tolerance and 58 with impaired glucose tolerance ) were evaluated an average of 2.6 years after baseline . weight gain was significantly correlated with declining di in both normal glucose tolerance and impaired glucose tolerance ; the correlation was stronger in the subgroup with impaired glucose tolerance ( 5 ) . in kriketos 's study , 20 women with a family history of type 2 diabetes ( risk group ) and 15 women without a family history of type 2 diabetes ( control group ) were tested at baseline and 6 years later . insulin resistance and secretion were assessed by homeostasis model assessment ( homa)-r and homa-. baseline levels and changes over time in total and central abdominal fat mass were inversely related to homa- ( 6 ) . our study provides novel information about the potential mediation of declining -cell function associated with weight gain . some may be due to increasing insulin resistance , which is not surprising given the beneficial effects of chronic use of insulin - sensitizing medications on -cell function in this population ( 13,15 ) . additional contributors may be endocrine and/or inflammatory products of adipose tissue , a novel finding to our knowledge . epidemiological studies have revealed direct associations between diabetes risk and markers of inflammation ( 16 ) and inverse associations between diabetes risk and adiponectin levels ( 17,18 ) . in vitro studies reveal evidence for detrimental effects of cytokines ( 19 ) and beneficial effects of adiponectin ( 20 ) on -cells in the short term . the present study demonstrated longitudinal association between adipocyte - derived hormones and declining -cell compensation for insulin resistance in humans . the results raise the possibility that circulating products of adipose tissue such as adiponectin and inflammatory cytokines are harmful to -cells . we did not find evidence for an effect of leptin , il-6 , ffa , baseline level of glucose , cholesterol , triglyceride , insulin resistance , insulin secretion , and -cell compensation on changes in -cell compensation in this cohort . the lack of impact of initial glucose levels is consistent with other studies we have conducted in this high - risk group ( 13 ) and speaks against glucotoxicity as a major cause of declining -cell function in pre - diabetic individuals . the lack of an effect of lipid levels , including ffas , speaks against lipotoxicity , although circulating lipid levels obtained in the fasting state may not be a good indicator of lipid delivery to or metabolism by -cells . the lack of an effect of initial insulin resistance , secretion , and -cell compensation after correcting for the bias due to measurement error did not support the hypothesis that high insulin resistance , high insulin secretion , and -cell compensation lead to faster declining of -cell compensation in this high - risk , very insulin - resistant group . we recognized that the relative small sample size in this report limited our ability to identify small effects of any of these factors on -cell compensation . our observation is consistent with results of several type 2 diabetes prevention trials in which the best evidence for slowing or arrest of declining -cell function comes from interventions that either reduce body weight ( 21 ) or change the biology of fat ( 13,15,22 ) . the role of inflammation and adipokines in diabetes prevention in these studies remains to be determined . however , studies using aspirin and salcylate ( 23,24 ) and il-1 receptor antagonist anakinra ( 25 ) indicate that treatment of inflammation per se may have beneficial effects on glucose regulation in pre - diabetic and diabetic humans . in summary , we found a significant association between weight gain and declining -cell compensation for insulin resistance in hispanic women at high risk for type 2 diabetes . the effect was explained in part by changes in insulin resistance and in part by changes in circulating adiponectin and crp . the results raise the possibility that increased body fat contributes to declining -cell function not only through insulin resistance and increased demands on -cells ( 13,15 ) , but also through direct effects of circulating adipose tissue peptides on -cell replication and/or survival . our findings highlight the importance of reducing body fat and/or its detrimental metabolic effects to preserve pancreatic -cell function and prevent or arrest the progression to / of diabetes in high - risk individuals .
objectiveto identify factors associated with declining -cell compensation for insulin resistance.research design and methodsin a cohort of hispanic women with recent gestational diabetes mellitus , oral glucose tolerance tests ( ogtts ) , intravenous glucose tolerance tests ( ivgtts ) , and bioelectrical impedance measurements were performed at 15-month intervals for up to 5 years , or until fasting plasma glucose exceeded 140 mg / dl ( 7.8 mmol / l ) . 
 data were analyzed to identify predictors of declining -cell compensation for insulin resistance ( the disposition index [ di ] ) and to examine the mechanism of weight gain and changes in circulating levels of selected adipokines and inflammatory markers on -cell compensation decline.resultsa total of 60 nondiabetic women had a median of four sets of ogtt + ivgtt during a median follow - up of 52 months . 
 fourteen of the women developed diabetes . 
 none of the baseline characteristics were significantly predictive of a decline in di . 
 there were significant univariate associations between declining di and weight gain ( specifically fat gain ) , declining adiponectin and rising c - reactive protein . 
 multivariate analysis showed that the weight gain was the most significant factor associated with declining di . 
 the amount of association between weight gain and declining di was explained 31% by changes in adiponectin and c - reactive protein and 40% by changes in insulin resistance.conclusionsthese results identify weight gain as the strongest factor associated with declining -cell compensation for insulin resistance in hispanic women at high risk for type 2 diabetes . 
 such effect may be mediated through at least two effects : alterations in adipokine levels and increasing insulin resistance .