motor and sensory losses can be seen in affected patients and include dizziness , balance disorders , fainting , loss of consciousness , nonepileptic seizures , dysphagia , aphonia , anosmia , loss of vision , double vision , paresthesia , anesthesia , visible symptoms , and paralysis.2,3 cd is most frequently seen in patients aged between 15 years and 35 years.4 the etiology is not fully understood ; however , it is thought that sex , psychological stress , intrafamilial conflict , childhood trauma , low socioeconomic status , and problems encountered in the social environment play a role in the emergence of the disease . when these systems are unbalanced in favor of oxidants , oxidative stress results.5 it has been shown that disorders involving the release of free radicals or failure of the antioxidant defense system may play a role in the pathophysiology of neuropsychiatric disorders.6 to date , impaired oxidative stability has been associated with depression , anxiety , attention - deficit hyperactivity disorder ( adhd ) , obsessive  compulsive disorder ( ocd ) , and nocturnal enuresis.711 s100b protein is secreted by the glial cells of the brain and acts to modulate the proliferation and alteration of neurons.12 s100b has neurotrophic and neuroprotective effects at physiological nanomolar concentrations , but the higher concentrations that occur following astrocytic death have been shown to be neurotoxic . specifically , there is a relationship between s100b protein levels and neurodegenerative diseases , such as alzheimer s disease , mood disorders ( major depression and bipolar disorder ) , and schizophrenia.13 it has also been shown that the blood levels of s100b protein increase after damage to the cells of the brain . some studies on schizophrenia have demonstrated increased s100b protein levels during the acute phase of the disease.14,15 these data raise the question of whether there is a relationship among cd , oxidative metabolism , and s100b protein levels because this condition is more often seen in people exposed to intensely inadequate psychosocial conditions that increase stress and cause a variety of emotional problems . when we searched the literature , we were unable to find any reports concerning a potential relationship between oxidative metabolism and s100b protein levels . however , the etiology of cd is not fully known ; symptoms such as motor and sensory deficits , nonepileptic seizures , and exposure to increased stress directed us to investigate the potential relationship between oxidative stress and cd . in this study , we evaluated the oxidative  antioxidative status and s100b protein levels in people with cd . thirty - seven patients ( 12 males and 25 females ; age range , 1860 years ) admitted to the emergency department with a diagnosis of cd in accordance with the turkish version of the diagnostic and statistical manual of mental disorders , 4th edition criteria between may and august 2015 were included in this study . all physical examination findings were normal , and there were no evident organic pathologies or history of drug use . patients were diagnosed with cd by a psychiatrist using a structured psychiatric interview based on the diagnostic and statistical manual of mental disorders , 4th edition . forty - two healthy volunteers ( 21 females and 21 males ) , matched for age and sex of the experimental group , were included in this study . exclusion criteria included mental retardation , alcohol use , tobacco use , depression , anxiety , and ecstasy use . patients with epilepsy , migraine , multiple sclerosis , parkinson s disease , schizophrenia and other psychotic disorders , and dementia and other cognitive disorders were also excluded from the study . drug , alcohol , tobacco , and psychoactive substance users were excluded from the control group . approval for the study was granted by the harran university institutional ethics committee , and written informed consent was obtained from all participants . each patient s complete blood count was evaluated using an automatic blood counting device ( abbott cell dyn 3500 ; abbott laboratories , abbott park , il , usa ) . blood samples taken for biochemical analyses were centrifuged at 3,500 rpm for 5 minutes , and the cellular components were discarded . serum samples were stored at 80c until analysis . the serum total antioxidant status ( tas ) and total oxidant status ( tos ) were measured using a colorimetric autoanalyzer ( abbott aeroset ; abbott laboratories ) , and these levels were used to calculate the oxidative stress index ( osi ) . plasma s100b levels were measured using an elisa kit ( biovendor laboratorni medicina , brno , czech republic ) . serum tas was measured using an automated method previously defined by erel.16 in the fenton reaction , a ferrous ion solution ( reagent 1 ) is mixed with a hydrogen peroxide solution ( reagent 2 ) , resulting in the release of potent hydroxyl radicals . brown - colored dianisidinyl radical cations , which are also potent radicals , are subsequently produced by the hydroxyl radicals . this assay determines the antioxidative capacity of the sample against the potent free radical reactions triggered by the hydroxyl radical . measurement of the serum tos level was performed using a new automated method described by erel.17 the ferrous ion  o - dianisidine complex is oxidized by the oxidants present in the sample , yielding ferric ions . the intensity of the color , which can be measured spectrophotometrically , is related to the total quantity of oxidant molecules present in the sample . the osi is defined as the ratio of the tos to tas and is expressed as a percentage . following normalization of the tos and tas units , the osi value was calculated based on the following formula : osi ( arbitrary units ) = tos ( mol h2o2 equiv / l)/tas ( mmol trolox equiv / l ) 10.17 commercially available elisa kits ( biovendor laboratorni medicina ) were used to measure serum s100b levels . briefly , the samples were incubated with a biotin - labeled monoclonal antihuman s100b antibody for 120 minutes and then washed . the samples were then transferred to elisa plate wells and incubated for 60 minutes with captured s100b antibody . the plate was then washed , and streptavidin - horse radish peroxidase enzyme conjugate was added to the wells . after a 30-minute incubation , a final wash was performed . conjugated horse radish peroxidase enzyme remaining in the plate was then allowed to react with the substrate solution ( tmb ) . differences between the two groups were assessed using an unpaired t - test . a p - value of < 0.05 was considered as statistically significant . analyses were performed using spss 11.5 statistical software ( spss inc . , chicago , il , usa ) . each patient s complete blood count was evaluated using an automatic blood counting device ( abbott cell dyn 3500 ; abbott laboratories , abbott park , il , usa ) . blood samples taken for biochemical analyses were centrifuged at 3,500 rpm for 5 minutes , and the cellular components were discarded . serum samples were stored at 80c until analysis . the serum total antioxidant status ( tas ) and total oxidant status ( tos ) were measured using a colorimetric autoanalyzer ( abbott aeroset ; abbott laboratories ) , and these levels were used to calculate the oxidative stress index ( osi ) . plasma s100b levels were measured using an elisa kit ( biovendor laboratorni medicina , brno , czech republic ) . serum tas was measured using an automated method previously defined by erel.16 in the fenton reaction , a ferrous ion solution ( reagent 1 ) is mixed with a hydrogen peroxide solution ( reagent 2 ) , resulting in the release of potent hydroxyl radicals . brown - colored dianisidinyl radical cations , which are also potent radicals , are subsequently produced by the hydroxyl radicals . this assay determines the antioxidative capacity of the sample against the potent free radical reactions triggered by the hydroxyl radical . measurement of the serum tos level was performed using a new automated method described by erel.17 the ferrous ion  o - dianisidine complex is oxidized by the oxidants present in the sample , yielding ferric ions . the intensity of the color , which can be measured spectrophotometrically , is related to the total quantity of oxidant molecules present in the sample . the osi is defined as the ratio of the tos to tas and is expressed as a percentage . following normalization of the tos and tas units , the osi value was calculated based on the following formula : osi ( arbitrary units ) = tos ( mol h2o2 equiv / l)/tas ( mmol trolox commercially available elisa kits ( biovendor laboratorni medicina ) were used to measure serum s100b levels . briefly , the samples were incubated with a biotin - labeled monoclonal antihuman s100b antibody for 120 minutes and then washed . the samples were then transferred to elisa plate wells and incubated for 60 minutes with captured s100b antibody . the plate was then washed , and streptavidin - horse radish peroxidase enzyme conjugate was added to the wells . after a 30-minute incubation , a final wash was performed . conjugated horse radish peroxidase enzyme remaining in the plate was then allowed to react with the substrate solution ( tmb ) . differences between the two groups were assessed using an unpaired t - test . a p - value of < 0.05 was considered as statistically significant . analyses were performed using spss 11.5 statistical software ( spss inc . , chicago , il , usa ) . twenty - five ( 68% ) of the 37 patients were females , with a mean age of 32.4912.59 years ( age range , 1860 years ) . twenty - one ( 50% ) of the 42 healthy volunteers enrolled in the study were females , with a mean age of 28.888.79 years ( age range , 1962 years ) . there were no statistically significant differences in age , sex , education level , or occupation between the patient and control groups ( p>0.05 for all ) ( table 1 ) . the tos , osi , and s100b values were significantly higher in the patient group than in the control group ( p<0.001 , p<0.001 , and p=0.007 , respectively ) . conversely , the tas levels of patients were significantly lower than those of controls ( p=0.014 ) ( table 2 ) . the key finding of our study is that the tos values of the patient group were significantly higher than those of the control group . several similar studies in the literature have reported increased tos values in association with many psychiatric disorders . the tos values were found to be elevated in two studies of schizophrenia and schizoaffective disorder.18,19 similarly , high tos values were reported in studies of major depression , bipolar disorder , adult and pediatric adhd , and childhood ocd.9,10,2022 in contrast , low tos values were reported in a study conducted in adult patients with ocd.23 increased tos levels in patients with cd indicate that oxidative stress is increased in these patients . our second most important finding is that the tas values of the patient group were significantly lower than those of the control group . this is consistent with similar studies that reported lower tas values in patients with generalized anxiety disorder , schizophrenia , major depression , bipolar disorder , childhood adhd and hyperactivity disorder , and ocd than in the general population.810,19,24,25 conversely , increased tas values were reported in association with bipolar disorder , adult adhd , and panic disorder.21,26,27 we believe that the high tos and low tas values observed in our study are related to the inability of patients with cd to activate antioxidant systems sufficiently to deal with conditions of increased stress . another finding of our study is that the osi was significantly higher in patients with cd than in the control group . osi is accepted as an indicator of oxidative stress and is expressed as the ratio of tos to tas.28 the osi was also reported to be significantly elevated in similar studies of pediatric patients with ocd and adhd , and adult patients with generalized anxiety disorder.810 an explanation for the high osi may be that the antioxidant levels could not be elevated sufficiently to fully remove stressors from the organism . finally , the s100b protein levels were significantly higher in the patient group than in the control group . s100b protein is expressed by astrocytes , and although it plays a protective role against neuronal damage under normal conditions , it may exacerbate neuronal damage at higher concentrations.29 high levels of s100b inhibit central nervous system function and may be indicative of central nervous system damage or defects.30 in the literature , s100b is used as an important biochemical marker for brain damage in patients with psychiatric disorders . elevated s100b levels in the cerebrospinal fluid were found in patients with schizophrenia , major depression , neurodegenerative diseases , and the acute phase of bipolar disorder.3133 in vitro , it has been shown that s100b induces oxidative stress . both structural and functional changes in subcortical structures , such as the thalamus and the basal ganglia , were detected in these patients . additionally , functional disorders of the striato - cortico - thalamic circuit , which controls voluntary motor movements and sensorimotor function , were observed in these patients.34,35 we believe that increased levels of s100b may be indicative of neuronal damage in patients with cd . the increased tos and osi levels and the decreased tas levels found in this study show that oxidative stress is one of the factors that contributes to the emergence of cd , and the antioxidant response to oxidative stress in patients with cd is inadequate . our study is the first in the literature to report these findings and should be supported by additional studies . the limitations of our study include its small sample size and its cross - sectional design . the strength of our study is that this is the first study aiming to explain the biological causes of a disease with primarily psychological symptoms . without longitudinal studies and large sample sizes , it will be challenging to interpret the effect of oxidative stress on patients with cd .
introductionvarious psychodynamic , neurobiological , genetic , and sociocultural factors are believed to be involved in the etiology of conversion disorder ( cd ) . 
 oxidative metabolism has been shown to deteriorate in association with many health problems and psychiatric disorders . 
 we evaluated oxidative metabolism and s100b levels in the context of this multifactorial disease.methodsthirty-seven patients with cd ( 25 females and 12 males ) and 42 healthy volunteers ( 21 females and 21 males ) , all matched for age and sex , were included in this study . 
 the total oxidant status , total antioxidant status , oxidative stress index , and s100b levels were compared between the two groups.resultsthe total oxidant status , oxidative stress index , and s100b levels were significantly higher in patients with cd than in the control group , whereas the total antioxidant status was significantly lower.conclusioncd is associated with deterioration of oxidative metabolism and increased neuronal damage .