for differentiation , caco-2 cells were plated on polycarbonate transwell filter inserts of 0.4  m pore size ( costar , madrid , spain ) in 6-well plates . approximately 14  days after confluence cells develop morphologic and physiologic characteristics of normal enterocytes . cholesterol ( chol ) , cla and dha ( sigma , madrid , spain ) were delivered to cells as micelles with lyso - phosphatidylcholine ( lyso - pc ; sigma , madrid , spain ) and sodium taurocholate ( tau ; sigma , madrid , spain ) . briefly , chol ( 250  m ) , cla ( 200  m ) or dha ( 200  m ) were diluted in n - hexane ( scharlab ; barcelona , spain ) and dried under a nitrogen stream . lyso - pc ( 200  m ) was also diluted in n - hexane and mixed with the dried chol , cla or dha , respectively . tau ( 5  mm ) was diluted in ethanol 95% and added to the lyso - pc / lipid mixture and then evaporated under a nitrogen stream . the resulted micelles were diluted in a serum - free medium and mixed vigorously until clear . as control , we used empty micelles made with lyso - pc and tau . four control and cholesterol samples , three dha samples and two cla samples were analyzed . microrna - enriched total rna was isolated with mirneasy mini kit ( qiagen , madrid , spain ) following manufacturer 's instructions . total rna concentration was quantified with a nanodrop and the integrity was confirmed in a 1% agarose gel . the expression of micrornas contained in the mirbase version 17 was analyzed by microarray using the paraflomicrofluidic biochip technology of lcsciences ( huston , tx , usa ) . a total of 1  g of mirna - enriched rna was used for mirna microarray . at least three samples of each condition were analyzed ( except for cla , for which we processed two biological replicates ) . raw data corresponding to signal intensity were corrected for background subtraction and normalized using the lowess ( locally weighted regression ) method . data were processed to merge replicates and discard discordant replicates ( 1-fold standard deviation ) . the resulting fold change ( fc ) was log2 transformed and analyzed for statistical significance using t - test and applying multiple testing corrections . microarray data has been submitted to the geo database complying with the minimal information about a microarray experiment ( miame ) guidelines ( gse59153 ) . significant micrornas were clustered using average linkage algorithm and euclidean distance correlation with tigermev software ( boston , ma , usa ) ( fig .
we performed a screening of mirnas regulated by dietary lipids in a cellular model of enterocytes , caco-2 cells . 
 our aim was to describe new lipid - modified mirnas with an implication in lipid homeostasis and cardiovascular disease [ 1 ] , [ 2 ] . 
 for that purpose , we treated differentiated caco-2 cells with micelles containing the assayed lipids ( cholesterol , conjugated linoleic acid and docosahexaenoic acid ) and the screening of mirnas was carried out by microarray using the paraflomicrofluidic biochip technology of lc sciences ( huston , tx , usa ) . 
 experimental design , microarray description and raw data have been made available in the geo database with the reference number of gse59153 . 
 here we described in detail the experimental design and methods used to obtain the relative expression data .