nepenthes spp . plants are one of passive carnivorous genus which lack in molecular genetics information.the lack of a complete transcript database from this genus hinders new protein discovery through proteomics approach . hence , these data sets help in the exploration of novel genes / proteins to understand carnivory in pitcher plants , and more generally in carnivorous plants.these data are also important for the identification of unique digestive enzymes and aspartic proteinases from pitcher plant . this will improve our understanding on the evolutionary history of this family of carnivorous plants . the lack of a complete transcript database from this genus hinders new protein discovery through proteomics approach . hence , these data sets help in the exploration of novel genes / proteins to understand carnivory in pitcher plants , and more generally in carnivorous plants . these data are also important for the identification of unique digestive enzymes and aspartic proteinases from pitcher plant . this will improve our understanding on the evolutionary history of this family of carnivorous plants . transcriptome profile of n.    ventrata were generated from the polya - enriched cdna libraries prepared from total rna extracted from its pitcher . the short reads were filtered , processed , assembled and analyzed as describe in the next section . raw data for this project were deposited at sra database with the accession numbers srx1389337 ( http://www.ncbi.nlm.nih.gov/sra/srx1389337 ) for day 0 control , srx1389392 ( http://www.ncbi.nlm.nih.gov/sra/srx1389392 ) for day 3 longevity experiment , and srx1389395 ( http://www.ncbi.nlm.nih.gov/sra/srx1389395 ) for day 3 chitin - treatment experiment . n.    ventrata pitcher plants were grown under shady environment in experimental plot ( 25509.0n 1014704.8e ) at universiti kebangsaan malaysia , bangi . whole pitchers were collected and freeze in liquid nitrogen before stored in   80  c for further use . three different pitcher samples were collected , namely day 0 control , day 3 longevity and day 3 chitin - treated . day 0 control sample was collected with 24  h of pitcher opening . for longevity experiment to understand the effect of time and protein depletion after pitcher opening on gene expression , day 0 pitcher fluids were syringe filtered through 0.22  m pvdf membrane followed by protein concentration at 10,000 molecular weight cutoff ( mwco ) . the pitchers were then replenished by the filtrate with depleted protein bigger than 10  kda , sealed by parafilm , and collected after 3  days . for chitin treatment experiment , 30  mm ( w / v ) of chitin were added into the pitcher fluid upon day 0 pitcher opening , sealed by parafilm , and collected after 3  days . for rna works , quantity and integrity of extracted total rna were determined using nanodrop ( thermo fisher scientific inc . , one pitcher for each treatment of n.    ventrata was sequenced using the illumina hiseq 2500 sequencing platform . paired end reads of 125  bp was generated through the standard polya - enriched library preparation protocol implemented by macrogen , south korea . raw reads from all three data sets were filtered to remove adapter sequences with sequence pre - processing tool , trimmomatic . high quality illumina raw reads with phred score    25 were kept for assembly . protein coding sequences of unique transcripts were analyzed via transdecoder version v2.0.1 as a part of trinity analysis pipeline . standard trinotate ( v2.0.0 ) annotation pipeline ( https://trinotate.github.io/ ) was carried out to annotate the assembled unique transcripts against swissprot , pfam , eggnog , gene ontology , signalp , and rnammer . n.    ventrata pitcher plants were grown under shady environment in experimental plot ( 25509.0n 1014704.8e ) at universiti kebangsaan malaysia , bangi . whole pitchers were collected and freeze in liquid nitrogen before stored in   80  c for further use . three different pitcher samples were collected , namely day 0 control , day 3 longevity and day 3 chitin - treated . day 0 control sample was collected with 24  h of pitcher opening . for longevity experiment to understand the effect of time and protein depletion after pitcher opening on gene expression , day 0 pitcher fluids were syringe filtered through 0.22  m pvdf membrane followed by protein concentration at 10,000 molecular weight cutoff ( mwco ) . the pitchers were then replenished by the filtrate with depleted protein bigger than 10  kda , sealed by parafilm , and collected after 3  days . for chitin treatment experiment , 30  mm ( w / v ) of chitin were added into the pitcher fluid upon day 0 pitcher opening , sealed by parafilm , and collected after 3  days . for rna works , rna from all samples were extracted using modified method of ctab . quantity and integrity of extracted total rna were determined using nanodrop ( thermo fisher scientific inc . , one pitcher for each treatment of n.    ventrata was sequenced using the illumina hiseq 2500 sequencing platform . paired end reads of 125  bp was generated through the standard polya - enriched library preparation protocol implemented by macrogen , south korea . raw reads from all three data sets were filtered to remove adapter sequences with sequence pre - processing tool , trimmomatic . high quality illumina raw reads with phred score    25 were kept for assembly . protein coding sequences of unique transcripts were analyzed via transdecoder version v2.0.1 as a part of trinity analysis pipeline . standard trinotate ( v2.0.0 ) annotation pipeline ( https://trinotate.github.io/ ) was carried out to annotate the assembled unique transcripts against swissprot , pfam , eggnog , gene ontology , signalp , and rnammer .
carnivorous plants have the ability to capture and digest insects for nutrients , which allows them to survive in land deprived of nitrogenous nutrients . 
 nepenthes spp . 
 are one of the carnivorous plants , which uniquely produce pitcher from the tip of an elongated leaf . 
 this study provides the first transcriptome resource from pitcher of a nepenthes ventricosa    nepenthes alata hybrid , nepenthes    ventrata to understand carnivory mechanism in nepenthes spp . 
 , as well as in other carnivorous species . 
 raw reads and the transcriptome assembly project have been deposited to sra database with the accession numbers srx1389337 ( day 0 control ) , srx1389392 ( day 3 longevity ) , and srx1389395 ( day 3 chitin - treated ) .