in recent years , technological advancement and technical improvement have lowered morbidity and mortality rates of many neurosurgical procedures.13 however , dural repair and reconstruction after major operations remain a challenge for neurosurgeons.4,5 it is well known that intact dura matters cover the brain surface and function as a natural barrier to prevent cerebrospinal fluid ( csf ) leakage . postoperative csf leakage is a life - threatening complication that can lead to various consequences , such as meningeal pseudocyst and arachnoiditis.68 currently , suture remains the most frequently adopted methods in dural repair regardless of the location of defects . however , suture operation , even dural destretching suture , is very difficult to be carried out , when dural defects are friable or located in relatively inaccessible areas.9 in particular , the prevalence of meningeal tear accompanied with spinal operations urges the discovery of more safe and efficient dural repair techniques . cyanoacrylates ( cas),10 fibrin glue,11 and co2 laser12 have been used to achieve dural closure , to prevent infections , and to provide a surface along which the dura can be regenerated . cas are the generic name of the ca - based  fast - acting  adhesives , such as methy-2-cyanoacrylate and ethyl-2-cyanoacrylate ( commonly sold under trade names like super glue ) . n - octyl-2-cyanoacrylate ( noca ) , an easily biocompatible and bioresorbable polymer glue , provides an alternative to conventional rigid fixation techniques.13 ca medical adhesives have been investigated for use as tissue adhesive in wound closure since 1959.14 compared to other materials , cas have several advantages , including convenience of use , relative painlessness , and no need of removal , which provide an acceptable cosmetic effect for external use.15 the ca tissue adhesives are liquid monomers that become self - polymerized in an exothermic reaction upon contact with tissue surfaces and form strong yet flexible film bondings on the apposed wound edges.16 the traditional methods to deliver noca adhesive include injection , daubing , and spraying , all of which are problematic . first , it is difficult to control the homogeneity and thickness of the coating layer with those traditional techniques . second , it is difficult to control the deposition range in adherence to surrounding tissue , which may cause serious tissue adhesion and inflammatory response after dural repair . furthermore , the tissue adhesions are associated with numerous postsurgical complications , including patient s pain , functional obstruction , and sometimes difficult reoperative surgery.17 electrospinning has been recognized as a highly facile and efficient nanotechnology to produce continuous fibers with diameters ranging from a few micrometers to a few nanometers.18 various polymers major in solvent solution and melt form have been successfully electrospun into ultrathin fibers in recent years.19 a thin charged jet is formed when the electrostatic force generated by a high operating voltage overcomes the surface tension of the polymer droplet during conventional eletrospinning . the jet is accelerated toward the grounded collector and produces fibers in the form of a nonwoven mat.20 with large surface - to - volume ratio and high porosity , electrospun nanofibers from natural and synthetic biomedical polymers can be made for applications in biomedical,2123 energy storage,24,25 environment engineering,2628 health care,29,30 biotechnology,31,32 etc . in this study , we attempt to develop noca ultrathin fibers by the solvent - free electrospinning technique , in which the noca monomers undergo self - assembly into micro / nanofibers in the presence of hydroxide ions . this study is expected to introduce for the first time a nonsuture dural repair technique via electrospun noca fibers . a novel gas - assisted electrospinning apparatus was self - designed to in situ electrospin noca fibers on the dura defects . after electrospinning , we characterized the morphology and structure of the noca fibers . in vitro experiment revealed that the self - assembled electrospun noca membrane has properties of high strength , good flexibility , and waterproofness without resulting in leakage even under a pressure of 78 mmhg . furthermore , we showed that the gas - assisted electrospinning apparatus could precisely deposit ultrafine fibers on the desired site to form a continuous and compact protective film and avoid tissue adhesion in the simulation experiment . medical glue ( otologic and craniocerebral glue series ) , mainly composed of noca and a little medical poly(methyl methacrylate ) as an additive ( in order to increase viscosity ) , was purchased from guangzhou baiyun medical adhesive co. , ltd ( guangzhou , people s republic of china ) and used directly without further treatments . a gas - assisted electrospinning apparatus is self - designed to electrospin noca fibers and achieve precise deposition for dural repair ( figure 1 ) . the apparatus consists of the following four parts : high - voltage power supply , syringe pump , air pump , and a three - way handle spinneret.33 the handle spinneret is connected to the positive electrode of the high - voltage power supply ( dw - p303 - 1acfo ; tianjin dongwen , tianjin , people s republic of china ) , which could provide 610 kv voltage to produce fibers . a syringe pump is applied to maintain a stable and precise feeding rate of the electrospun solution . in addition , to facilitate the electrospinning process and control the fiber deposition scope , we connected a gas pump to the auxiliary spinneret , which blows and exerts a stretching force on the electrospinning solution . the morphologies of the noca ultrathin fibers were characterized by optical microscope ( bx51 ; olympus corporation , tokyo , japan ) and scanning electron microscopy ( sem ; tm-1000 ; hitachi ltd . , the intermolecular structure was determined by fourier transform infrared ( ftir ) spectrometer ( nicolet in10 ; thermo fisher scientific , waltham , ma , usa ) . in vitro experiments were carried out on fresh bovine pericardium purchased from the market to study the flexibility and integrity of the noca nanofibrous membranes . in vitro simulation experiments were first carried out on eggs to determine the sealing property of the electrospun noca nanofibrous membranes . after removal of the outer shell , two kinds of common operative incisions were cut on egg membranes . furthermore , we carried out in vivo modeling experiment on goat brain in order to determine the efficacy of this nonsuture technique . all animal - handling procedures were performed according to the guide for the care and use of laboratory animals of the national institutes of health and followed the guidelines of the animal welfare act . all the procedures involving animal use were approved by the chancellor s animal research committee at qingdao university . a gas - assisted electrospinning apparatus is self - designed to electrospin noca fibers and achieve precise deposition for dural repair ( figure 1 ) . the apparatus consists of the following four parts : high - voltage power supply , syringe pump , air pump , and a three - way handle spinneret.33 the handle spinneret is connected to the positive electrode of the high - voltage power supply ( dw - p303 - 1acfo ; tianjin dongwen , tianjin , people s republic of china ) , which could provide 610 kv voltage to produce fibers . a syringe pump is applied to maintain a stable and precise feeding rate of the electrospun solution . in addition , to facilitate the electrospinning process and control the fiber deposition scope , we connected a gas pump to the auxiliary spinneret , which blows and exerts a stretching force on the electrospinning solution . the morphologies of the noca ultrathin fibers were characterized by optical microscope ( bx51 ; olympus corporation , tokyo , japan ) and scanning electron microscopy ( sem ; tm-1000 ; hitachi ltd . , the intermolecular structure was determined by fourier transform infrared ( ftir ) spectrometer ( nicolet in10 ; thermo fisher scientific , waltham , ma , usa ) . in vitro experiments were carried out on fresh bovine pericardium purchased from the market to study the flexibility and integrity of the noca nanofibrous membranes . in vitro simulation experiments were first carried out on eggs to determine the sealing property of the electrospun noca nanofibrous membranes . after removal of the outer shell , two kinds of common operative incisions were cut on egg membranes . furthermore , we carried out in vivo modeling experiment on goat brain in order to determine the efficacy of this nonsuture technique . all animal - handling procedures were performed according to the guide for the care and use of laboratory animals of the national institutes of health and followed the guidelines of the animal welfare act . all the procedures involving animal use were approved by the chancellor s animal research committee at qingdao university . figure 2 shows the morphologies of the noca fibers prepared by the gas - assisted electrospinning apparatus . different from the conventional electrospinning and spraying processes , the electrospun noca fibers were rapidly polymerized into a solid self - assembled membrane on the surface of wound when a high voltage was applied . in our present electrospinning process , the applied high voltage was 10 kv , and the distance from the spinneret to the collector was 68 cm . the solution feeding rate was 15 l min with the airflow velocity of 12 l min . figure 2b shows the sem image of the electrospun noca coating on the goat dural , which was fabricated with a feeding rate of 25 l min in 30 seconds . the self - assembled noca nanofibers formed a very compact film and barrier preventing leakage . the structure of the electrospun noca polymer was characterized with ftir spectrum analysis ( figure 3 ) . the peaks at 2,250 cm and 1,750 cm are characteristic of the cn and c = o groups of the polymer , respectively . the significant decrease in = ch  stretching vibration ( 3,000 cm ) could be ascribed to the opened c = c bond of the vinyl structure in noca monomer unit . the presence of peak corresponding to the oh bond at 3,400 cm proved the hydroxyl group as an anionic initiator inducing polymerization . in addition , the peak corresponding to a great number of ch2 units in the main carbon chain at 724 cm was also found . the presence of cn stretching mode in the spectrum demonstrated the existence of noca in the electrospun fibers ( table 1 ) . due to the fragility and ossification of the dura , first , the bovine pericardium was chosen as a substitute of the dura , and a defect of 2 cm in length was made . then , the defect was covered by an electrospun noca layer using the gas - assisted electrospinning apparatus . one minute later , the membrane was bent to multiangle and recovered back to its original state without any breakage , indicating that the electrospun noca film had good strength , elasticity , and flexibility and thus met the requirements of dural closure and reconstruction . it has been reported that fibrous membrane of noca glue has high strength and strong tissue - bonding ability.31 we first evaluated the bonding strength and watertightness of the electrospun noca nanofibrous membranes in water leakage tests ( figure 5 ) . after mounting the bovine pericardium securely on the bottom of poly(methyl methacrylate ) pipe , we produced a defect with a scalpel . the defect was then covered by a layer of noca nanofiber film in 30 seconds using the gas - assisted electrospinning apparatus . strikingly , we found no water leakage for 12 hours under the pressure of 78 mmhg , indicating that the electrospun noca fiber membrane is compact and strong . further experiment showed that the electrospun noca nanofibrous membrane could stand as high as 125 mmhg pressure without water seeping , while the traditional sprayed noca film could only sustain a relatively low pressure of 38 mmhg . different from the conventional electrospinning and spraying , one remarkable characteristic of our self - designed gas - assisted electrospinning apparatus is precise deposition on a desired site , namely , the deposition range and deposition location can be precisely controlled under the assistance of gas . as shown in figure 6 , the deposition width was investigated with different distances . with an increase in electrospinning distance , the efficacy of electrospun noca membrane was first determined in the modeling experiment on eggs ( figure 7 ) . the outer shell of fresh eggs was removed , and two kinds of common wounds were created on the egg membranes : one wound with a width of 24 cm and the other with an area of 49 cm . the air pump was turned on before electrospinning , which could not only precisely direct electrospinning and deposition but also help to clean the collector surface . when a high voltage was applied , the electrospun noca fibers were deposited and rapidly polymerized on the wound surface to form a solid self - assembled membrane . after electrospun for 20 seconds , the dural wound was rapidly covered by a layer of noca fibrous film . no tissue fluid was observed when eggs were inverted 1 minute after wound closure , demonstrating that the eletrospun noca membrane is leakage proof . if an unintended dural rent or pinhole tear occurs , it is tamponaded to prevent csf loss . figure 8 presents the operation procedure of dural repair in a goat brain using the gas - assisted electrospinning apparatus . gross appearance of every stage in the simulation experiment was observed ( as shown in figure 8 and video s1 ) . figure 8a shows that a defect of 7 cm in length was produced by the scalpel . remarkably , considering the fragility of the large defect , the dura was primarily sutured to avert rupture ( figure 8b ) . then , the noca fibers were precisely electrospun onto the defect by the gas - assisted electrospinning apparatus ( figure 8c ) . consistently , we found that the dural defect was repaired by the noca membrane in 30 seconds . as shown in figure 8d , the in situ electrospun noca fibers can not only produce good sealing but also have precise deposition effectively avoiding tissue adhesion , which makes the procedure more secure and simple . the above operation was repeated in ten goat brains with similar result , indicating that this technique is reliable and replicable . figure 2 shows the morphologies of the noca fibers prepared by the gas - assisted electrospinning apparatus . different from the conventional electrospinning and spraying processes , the electrospun noca fibers were rapidly polymerized into a solid self - assembled membrane on the surface of wound when a high voltage was applied . in our present electrospinning process , the applied high voltage was 10 kv , and the distance from the spinneret to the collector was 68 cm . the solution feeding rate was 15 l min with the airflow velocity of 12 l min . figure 2b shows the sem image of the electrospun noca coating on the goat dural , which was fabricated with a feeding rate of 25 l min in 30 seconds . the self - assembled noca nanofibers formed a very compact film and barrier preventing leakage . the structure of the electrospun noca polymer was characterized with ftir spectrum analysis ( figure 3 ) . the peaks at 2,250 cm and 1,750 cm are characteristic of the cn and c = o groups of the polymer , respectively . the significant decrease in = ch  stretching vibration ( 3,000 cm ) could be ascribed to the opened c = c bond of the vinyl structure in noca monomer unit . the presence of peak corresponding to the oh bond at 3,400 cm proved the hydroxyl group as an anionic initiator inducing polymerization . in addition , the peak corresponding to a great number of ch2 units in the main carbon chain at 724 cm was also found . the presence of cn stretching mode in the spectrum demonstrated the existence of noca in the electrospun fibers ( table 1 ) . due to the fragility and ossification of the dura , first , the bovine pericardium was chosen as a substitute of the dura , and a defect of 2 cm in length was made . then , the defect was covered by an electrospun noca layer using the gas - assisted electrospinning apparatus . one minute later , the membrane was bent to multiangle and recovered back to its original state without any breakage , indicating that the electrospun noca film had good strength , elasticity , and flexibility and thus met the requirements of dural closure and reconstruction . it has been reported that fibrous membrane of noca glue has high strength and strong tissue - bonding ability.31 we first evaluated the bonding strength and watertightness of the electrospun noca nanofibrous membranes in water leakage tests ( figure 5 ) . after mounting the bovine pericardium securely on the bottom of poly(methyl methacrylate ) pipe , we produced a defect with a scalpel . the defect was then covered by a layer of noca nanofiber film in 30 seconds using the gas - assisted electrospinning apparatus . strikingly , we found no water leakage for 12 hours under the pressure of 78 mmhg , indicating that the electrospun noca fiber membrane is compact and strong . further experiment showed that the electrospun noca nanofibrous membrane could stand as high as 125 mmhg pressure without water seeping , while the traditional sprayed noca film could only sustain a relatively low pressure of 38 mmhg . different from the conventional electrospinning and spraying , one remarkable characteristic of our self - designed gas - assisted electrospinning apparatus is precise deposition on a desired site , namely , the deposition range and deposition location can be precisely controlled under the assistance of gas . as shown in figure 6 , the deposition width was investigated with different distances . with an increase in electrospinning distance , the efficacy of electrospun noca membrane was first determined in the modeling experiment on eggs ( figure 7 ) . the outer shell of fresh eggs was removed , and two kinds of common wounds were created on the egg membranes : one wound with a width of 24 cm and the other with an area of 49 cm . the air pump was turned on before electrospinning , which could not only precisely direct electrospinning and deposition but also help to clean the collector surface . when a high voltage was applied , the electrospun noca fibers were deposited and rapidly polymerized on the wound surface to form a solid self - assembled membrane . after electrospun for 20 seconds , the dural wound was rapidly covered by a layer of noca fibrous film . no tissue fluid was observed when eggs were inverted 1 minute after wound closure , demonstrating that the eletrospun noca membrane is leakage proof . if an unintended dural rent or pinhole tear occurs , it is tamponaded to prevent csf loss . figure 8 presents the operation procedure of dural repair in a goat brain using the gas - assisted electrospinning apparatus . gross appearance of every stage in the simulation experiment was observed ( as shown in figure 8 and video s1 ) . figure 8a shows that a defect of 7 cm in length was produced by the scalpel . remarkably , considering the fragility of the large defect , the dura was primarily sutured to avert rupture ( figure 8b ) . then , the noca fibers were precisely electrospun onto the defect by the gas - assisted electrospinning apparatus ( figure 8c ) . consistently , we found that the dural defect was repaired by the noca membrane in 30 seconds . as shown in figure 8d , the in situ electrospun noca fibers can not only produce good sealing but also have precise deposition effectively avoiding tissue adhesion , which makes the procedure more secure and simple . the above operation was repeated in ten goat brains with similar result , indicating that this technique is reliable and replicable . nocas were successfully electrospun into ultrathin fibrous film via the solvent - free electrospinning technique . the morphology and structure of the noca membrane were characterized by the sem and ftir . a home - made gas - assisted electrospinning apparatus was designed to realize precise and homogeneous deposit of noca ultrathin fibers onto the dural defects . the in vitro simulation experiment on egg membranes and in vivo experiment on goat brains showed that this flexible and compact self - assembled membrane could not only rapidly suture dural defects but also avoid tissue adhesion . compared with typical conventional spraying , the dosage of electrospinning method is much less and thus has lower heat dissipation . this technology may be very promising in surgery especially on dural repair with high sealing capacity and low tissue adhesion . however , further studies are needed to test the practicability of this technology and the applicability of other adhesive agents as well .
purposein this work , we propose an in situ precise electrospinning of medical glue fibers onto dural wound for improving sealing capability , avoiding tissue adhesion , and saving time in dural repair.methodsn-octyl-2-cyanoacrylate , a commercial tissue adhesive ( medical glue ) , can be electrospun into ultrathin fibrous film with precise and homogeneous deposition by a gas - assisted electrospinning device.resultsthe self - assembled n - octyl-2-cyanoacrylate film shows high compactness and flexibility owing to its fibrous structure . 
 simulation experiments on egg membranes and goat meninges demonstrated that this technology can repair small membrane defects quickly and efficiently.conclusionthis method may have potential application in dural repair , for example , working as an effective supplementary technique for conventional dura suture .