celli et al7 have recently introduced the use of the bode index , a multidimensional 10-point scale assessing four prognostic factors in copd patients , namely body mass index ( b ) , degree of airflow obstruction ( o ) , functional dyspnea ( d ) , and exercise capacity ( e ) as assessed by the six - minute walk test ( 6mwt ) . the bode index has been extensively validated and shown to predict mortality7 and future hospitalizations.8 in addition , it has been found to be a good predictor of exacerbations9 and health - related quality of life.10 however , several modifications of the original bode index have been proposed to obtain novel indexes that may be more accurate and easier to use.11,12 in this regard , the 6mwt used in the original bode index is a submaximal test , is dependent on individual motivation , and may not express the real physical capacity of the patient . interestingly , correlations with the maximal oxygen uptake ( vo2max ) are only moderate.13 in contrast , maximal cardiopulmonary exercise testing ( cpet ) is considered the gold standard to assess maximal exercise tolerance . in particular , vo2max is an objective measure of physical fitness and might serve as a physical training target,14 a predictor of survival rates,15 and a disability index for copd patients.16 of note , the original bode index has been recently correlated with two modified bode indexes replacing 6mwt with vo2max expressed either as the percentage of predicted values ( mbode% ) or as absolute values in ml / min / kg ( mbode).17 intriguingly , the modified bode indexes have been recently linked to survival in copd patients.18 in addition , a highly significant correlation between the original bode and the modified indexes has been reported.17 it has been therefore suggested that the original bode index is highly effective in the evaluation of copd patients . although valuable data have been presented  including the use of vo2max in the bode index  an analysis of the agreement between the two modified bode indexes has not yet been performed . therefore , the question as to whether these indexes may have the same properties remains open . the purpose of this study was to analyze the agreement between the two modified bode indexes in a group of patients with stable copd . we also sought to investigate the relation between the modified bode indexes and the original bode in a subsample of patients . all procedures were approved by the local institution review board and written informed consent was obtained from all participants . patients fulfilling the following inclusion criteria were invited to participate in the study : a ) being an adult patient diagnosed as having copd according to international guidelines19 and b ) being clinically stable during the previous three months . exclusion criteria were : a ) recent exacerbations ( in the previous three months ) requiring systemic corticosteroids or antibiotics,20 and b ) any contraindication or inability to perform any of the study tests ( uncontrolled heart disease , acute pulmonary disease , and other conditions).14 medications already prescribed were continued during the study . patients underwent a complete clinical and functional evaluation followed by a maximal cpet on a cycle ergometer . to be able to compare our findings with those achieved using the original bode index , one out of every three patients performed the 6mwt according to international recommendations.21 clinical and functional evaluation consisted of dyspnea scoring , nutritional evaluation , spirometric testing , and arterial blood - gas analysis . basal dyspnea was assessed using the modified medical research council ( mrc ) scale.22 this is a five - point scale based on degrees of various physical activities that precipitate dyspnea . bmi was obtained by dividing body weight in kilograms by height in square meters ( kg / m ) . a bmi of less than 21.0 kg / m was considered to be underweight.7 spirometric investigations were carried out on a pneumotachograph spirograph masterlab ( erich jaeger gmbh , wuerzburg , germany ) , following american thoracic society ( ats ) recommendations.23 for the purpose of this study , values of forced expiratory volume in one second ( fev1 ) were recorded . blood - gas analyses were carried out with an abl-700 analyser ( radiometer , copenhagen , denmark ) using arterial blood samples taken from the radial or humeral artery at rest while the patient was breathing room air . arterial oxygen partial pressure ( pao2 ) carbon dioxide partial pressure ( paco2 ) and ph were recorded following the manufacturer s instructions and in keeping with spanish society of pulmonology and thoracic surgery recommendations.24 maximal cpet was performed on a cycle ergometer ( collins respiratory ; ergomed , seattle , wa , usa ) . maximal exercise tolerance was measured by a symptom - limited graded exercise test performed while the patient was breathing room air . we determined the vo2peak and carbon dioxide production , an indirect measure of anaerobic threshold . vo2peak was expressed as an absolute value or as a percentage of the predicted value ( in ml / min / kg ) . an average of the last 20 seconds of each minute was used for the purpose of analysis . the respiratory pattern ( maximal breathing capacity , tidal volume , respiratory rate ) during exercise was carefully recorded . data for ventilation and gas analysis were instantly obtained and analyzed breath - by - breath by a computer connected with the cycle ergometer . patients were monitored for three minutes at rest and then began to pedal without any resistance for one minute . the level of resistance was then increased by 1015 watts every minute until the maximal exercise level was reached . dyspnea , leg fatigue , and chest pain were assessed after exercise according to a modified borg s scale.25 the 6mwt was performed a few days before cpet following the current recommendations.21 prior to the index test , a trial test was performed to avoid a training effect . the two mbode indexes were calculated using bmi , fev1 , and mrc as in the original index.7 however , the use of 6mwt was replaced with vo2peak values , expressed either in ml / min / kg ( mbode ) or as the percentage of predicted ( mbode% ) . each variable was rated on a four - point ( 03 ) scale , the only exception being bmi , which was coded as a dichotomous variable ( 0 or 1 ) . the scale was then divided into quartiles , with the first quartile corresponding to scores of 02 points , the second quartile to scores of 34 points , the third quartile to scores of 56 points , and the fourth quartile to scores of 710 points.7 although several different stratifications may be theoretically used , vo2peak values were stratified as proposed by cardoso et al,17 to make results comparable . the values of vo2peak in ml / min / kg were therefore stratified according to previous methodology26 as follows : score 0  25 ml / min / kg ; score 1 = 2025 ml / min / kg ; score 2 = 1520 ml / min / kg ; score 3  15 ml / min / kg . similarly , values of vo%2peak predicted were stratified27 as follows : score 0  70%pred ; score 1 = 60%69%pred ; score 2 = 40%59%pred ; score 3  40%pred . statistical analysis was performed using the statistical package for social sciences ( version 15.0 ; spss inc . , chicago , il , usa ) , qualitative variables are expressed as absolute and relative frequencies . for descriptive purposes , variables are presented for the entire study cohort and according to gold stages.19 gold stages i and ii were unified within one group since only eight patients had stage i disease . comparisons between qualitative variables were performed using the  test or the fisher s exact test . agreement between the mbode and mbode% was studied using the kappa coefficient ( k ) according to the quartiles of the two mbode indexes . the mean differences between measures are reported as means  2 standard deviations in parentheses.28 the alpha error was set at 0.05 . patients fulfilling the following inclusion criteria were invited to participate in the study : a ) being an adult patient diagnosed as having copd according to international guidelines19 and b ) being clinically stable during the previous three months . exclusion criteria were : a ) recent exacerbations ( in the previous three months ) requiring systemic corticosteroids or antibiotics,20 and b ) any contraindication or inability to perform any of the study tests ( uncontrolled heart disease , acute pulmonary disease , and other conditions).14 medications already prescribed were continued during the study . patients underwent a complete clinical and functional evaluation followed by a maximal cpet on a cycle ergometer . to be able to compare our findings with those achieved using the original bode index , one out of every three patients performed the 6mwt according to international recommendations.21 clinical and functional evaluation consisted of dyspnea scoring , nutritional evaluation , spirometric testing , and arterial blood - gas analysis . basal dyspnea was assessed using the modified medical research council ( mrc ) scale.22 this is a five - point scale based on degrees of various physical activities that precipitate dyspnea . bmi was obtained by dividing body weight in kilograms by height in square meters ( kg / m ) . a bmi of less than 21.0 kg / m was considered to be underweight.7 spirometric investigations were carried out on a pneumotachograph spirograph masterlab ( erich jaeger gmbh , wuerzburg , germany ) , following american thoracic society ( ats ) recommendations.23 for the purpose of this study , values of forced expiratory volume in one second ( fev1 ) were recorded . blood - gas analyses were carried out with an abl-700 analyser ( radiometer , copenhagen , denmark ) using arterial blood samples taken from the radial or humeral artery at rest while the patient was breathing room air . arterial oxygen partial pressure ( pao2 ) carbon dioxide partial pressure ( paco2 ) and ph were recorded following the manufacturer s instructions and in keeping with spanish society of pulmonology and thoracic surgery recommendations.24 maximal cpet was performed on a cycle ergometer ( collins respiratory ; ergomed , seattle , wa , usa ) . maximal exercise tolerance was measured by a symptom - limited graded exercise test performed while the patient was breathing room air . we determined the vo2peak and carbon dioxide production , an indirect measure of anaerobic threshold . vo2peak was expressed as an absolute value or as a percentage of the predicted value ( in ml / min / kg ) . an average of the last 20 seconds of each minute was used for the purpose of analysis . the respiratory pattern ( maximal breathing capacity , tidal volume , respiratory rate ) during exercise was carefully recorded . data for ventilation and gas analysis were instantly obtained and analyzed breath - by - breath by a computer connected with the cycle ergometer . patients were monitored for three minutes at rest and then began to pedal without any resistance for one minute . the level of resistance was then increased by 1015 watts every minute until the maximal exercise level was reached . dyspnea , leg fatigue , and chest pain were assessed after exercise according to a modified borg s scale.25 the 6mwt was performed a few days before cpet following the current recommendations.21 prior to the index test , a trial test was performed to avoid a training effect . basal dyspnea was assessed using the modified medical research council ( mrc ) scale.22 this is a five - point scale based on degrees of various physical activities that precipitate dyspnea . bmi was obtained by dividing body weight in kilograms by height in square meters ( kg / m ) . spirometric investigations were carried out on a pneumotachograph spirograph masterlab ( erich jaeger gmbh , wuerzburg , germany ) , following american thoracic society ( ats ) recommendations.23 for the purpose of this study , values of forced expiratory volume in one second ( fev1 ) were recorded . blood - gas analyses were carried out with an abl-700 analyser ( radiometer , copenhagen , denmark ) using arterial blood samples taken from the radial or humeral artery at rest while the patient was breathing room air . arterial oxygen partial pressure ( pao2 ) carbon dioxide partial pressure ( paco2 ) and ph were recorded following the manufacturer s instructions and in keeping with spanish society of pulmonology and thoracic surgery recommendations.24 maximal cpet was performed on a cycle ergometer ( collins respiratory ; ergomed , seattle , wa , usa ) . maximal exercise tolerance was measured by a symptom - limited graded exercise test performed while the patient was breathing room air . we determined the vo2peak and carbon dioxide production , an indirect measure of anaerobic threshold . vo2peak was expressed as an absolute value or as a percentage of the predicted value ( in ml / min / kg ) . an average of the last 20 seconds of each minute was used for the purpose of analysis . the respiratory pattern ( maximal breathing capacity , tidal volume , respiratory rate ) during exercise was carefully recorded . data for ventilation and gas analysis were instantly obtained and analyzed breath - by - breath by a computer connected with the cycle ergometer . patients were monitored for three minutes at rest and then began to pedal without any resistance for one minute . the level of resistance was then increased by 1015 watts every minute until the maximal exercise level was reached . dyspnea , leg fatigue , and chest pain were assessed after exercise according to a modified borg s the 6mwt was performed a few days before cpet following the current recommendations.21 prior to the index test , a trial test was performed to avoid a training effect . the two mbode indexes were calculated using bmi , fev1 , and mrc as in the original index.7 however , the use of 6mwt was replaced with vo2peak values , expressed either in ml / min / kg ( mbode ) or as the percentage of predicted ( mbode% ) . each variable was rated on a four - point ( 03 ) scale , the only exception being bmi , which was coded as a dichotomous variable ( 0 or 1 ) . the scale was then divided into quartiles , with the first quartile corresponding to scores of 02 points , the second quartile to scores of 34 points , the third quartile to scores of 56 points , and the fourth quartile to scores of 710 points.7 although several different stratifications may be theoretically used , vo2peak values were stratified as proposed by cardoso et al,17 to make results comparable . the values of vo2peak in ml / min / kg were therefore stratified according to previous methodology26 as follows : score 0  25 ml / min / kg ; score 1 = 2025 ml / min / kg ; score 2 = 1520 ml / min / kg ; score 3  15 ml / min / kg . similarly , values of vo%2peak predicted were stratified27 as follows : score 0  70%pred ; score 1 = 60%69%pred ; score 2 = 40%59%pred ; score 3  40%pred . statistical analysis was performed using the statistical package for social sciences ( version 15.0 ; spss inc . , chicago , il , usa ) , qualitative variables are expressed as absolute and relative frequencies . for descriptive purposes , variables are presented for the entire study cohort and according to gold stages.19 gold stages i and ii were unified within one group since only eight patients had stage i disease . comparisons between qualitative variables were performed using the  test or the fisher s exact test . agreement between the mbode and mbode% was studied using the kappa coefficient ( k ) according to the quartiles of the two mbode indexes . the mean differences between measures are reported as means  2 standard deviations in parentheses.28 the alpha error was set at 0.05 . correlations between the two mbode indexes and the corresponding vo2peak value were good ( mbode index : r = 0.68 , p < 0.001 ; mbode% index : r = 0.75 , p < 0.001 ) . the correlation between the two mbode indexes was excellent ( r = 0.96 , p < 0.001 , figure 1 ) . however , the two mbode indexes were significantly different from each other ( table 1 ) . these differences were evident for the entire cohort ( p < 0.001 ) and throughout all gold stages ( kappa coefficients were significantly low in the entire study cohort ( k = 0.5 , p < 0.001 ) , but improved in patients with advanced gold stages ( gold i  ii : k = 0.49 , p < 0.001 ; gold iii : k = 0.3 , p < 0.001 ; gold iv : k = 0.69 , p < 0.001 ) . the mean differences according to gold stages were as follows : gold i  ii : 1.1 ( 0.42.63 ) ; gold iii : 1 ( 0.352.35 ) , and gold iv : 0.7 ( 0.51.98 ) units . in order to compare the mbode indexes with the original bode index , a subgroup of 54 patients ( table 2 ) performed the 6mwt ( mean distance 517  118 metres ) . there was a high correlation between original bode and both the mbode ( r = 0.88 ; p < 0.001 ) and mbode% ( r = 0.93 ; p < 0.001 ) indexes ( figure 3 ) . kappa coeff icients were low for both mbode - bode ( k = 0.12 ; p < 0.001 ) and mbode%-bode ( k = 0.38 ; p < 0.001 ) . results showed a mean difference in bode points of 1.8 ( 0.033.77 ) for mbode and 0.6 ( 1.12.32 ) for mbode% ( figure 4 ) . correlations between the two mbode indexes and the corresponding vo2peak value were good ( mbode index : r = 0.68 , p < 0.001 ; mbode% index : r = 0.75 , p < 0.001 ) . the correlation between the two mbode indexes was excellent ( r = 0.96 , p < 0.001 , figure 1 ) . however , the two mbode indexes were significantly different from each other ( table 1 ) . these differences were evident for the entire cohort ( p < 0.001 ) and throughout all gold stages ( kappa coefficients were significantly low in the entire study cohort ( k = 0.5 , p < 0.001 ) , but improved in patients with advanced gold stages ( gold i  ii : k = 0.49 , p < 0.001 ; gold iii : k = 0.3 , p < 0.001 ; gold iv : k = 0.69 , p < 0.001 ) . the mean differences according to gold stages were as follows : gold i  ii : 1.1 ( 0.42.63 ) ; gold iii : 1 ( 0.352.35 ) , and gold iv : 0.7 ( 0.51.98 ) units . in order to compare the mbode indexes with the original bode index , a subgroup of 54 patients ( table 2 ) performed the 6mwt ( mean distance 517  118 metres ) . there was a high correlation between original bode and both the mbode ( r = 0.88 ; p < 0.001 ) and mbode% ( r = 0.93 ; p < 0.001 ) indexes ( figure 3 ) . kappa coeff icients were low for both mbode - bode ( k = 0.12 ; p < 0.001 ) and mbode%-bode ( k = 0.38 ; p < 0.001 ) . results showed a mean difference in bode points of 1.8 ( 0.033.77 ) for mbode and 0.6 ( 1.12.32 ) for mbode% ( figure 4 ) . the present study demonstrates that the new modified bode indexes are significantly different from each other with a mean difference of around 1 bode point . these differences were less pronounced in the advanced stages of the disease , in which they were characterized by better agreement . moreover , the agreement with the original bode index was overall weak , and significantly lower for the mbode than for the mbode% . the original bode index is a simple multidimensional grading system which is superior to fev1 alone for prediction of mortality and hospitalization rates among copd patients . since its first publication in 20047 this index has been extensively used in different settings including pulmonary rehabilitation29 and lung volume reduction surgery.30 several attempts have recently been made to improve the original bode index , either by replacing or adding new components.11,12 in our study , two novel mbode indexes were constructed by replacing 6mwt with vo2peak expressed either as the percentage of predicted values ( mbode% ) or as the absolute values in ml / min / kg ( mbode ) . although the novel indexes thus far have not been studied in detail , preliminary evidence has indicated that the mbode% and the original bode index show similar prognostic properties.18 the identification of novel diagnostic tools is an important clinical goal . novel diagnostic tools or multicomponent measures are frequently being developed to improve prognostic models and to identify novel therapeutic targets for patients with copd . in this context , novel assessment tools need to be validated prospectively against pre - existing methodologies . two previous studies have investigated and validated the novel mbode indexes.17,18 to this end , a feasible approach is simple linear regression analysis , as previously done by cardoso et al.17 these authors have previously demonstrated a highly significant correlation between the original bode index and the two mbode indexes . however , it should be noted that the correlation between the original bode index and vo2max was only moderate.17 it is well known that good correlation does not necessarily imply good agreement and vice versa.28 therefore , the agreement between both indexes should be carefully evaluated by means of sound statistical analysis . the second report18 was a validation study of mbode% suggesting that this instrument has a good ability to predict mortality in a similar manner as the original bode . however , the authors found a difference of 1.4 points between the mbode% and the original bode . the study did not focus on the degree of agreement between the two measures . the present report expands previous studies on the mbode indexes by analyzing the degree of agreement between each other using the kappa and bland - altman plots.28 future longitudinal studies are needed to investigate whether the two new mbode indexes differ in their ability to predict mortality or exacerbations in patients with copd . although this issue has been partly addressed in the past,18 our study was not of prospective design and is therefore not suitable to confirm previous findings . in our study , the two mbode indexes showed a low degree of agreement , despite a high degree of correlation . interestingly , kappa coefficients were low for the entire study cohort and in all gold stages , improving in advanced stages . altman plot of difference versus mean values showed a difference of about 1 point among the two mbode indexes , which have proven to be a clinically relevant prognostic difference.31 two findings of our study merit consideration . it is widely recognized that patients with advanced copd suffer from severe impairment of functional capacity.32 in addition , the limitation in exercise capacity shows a lower variability in advanced stages characterized by a progressive reduction in the standard deviations of exercise variables ( see table 1).32 secondly , the degree of agreement with the original bode was different for the two mbode indexes . vo2peak is currently considered to be the best index of aerobic capacity and the gold standard for cardiorespiratory fitness . it represents the maximal achievable level of oxidative metabolism in large muscle groups.14 vo2peak is generally expressed as oxygen uptake in absolute units . however , vo2peak can be normalized for body weight to compare vo2peak among subjects with different anthropometric measures.33,34 of note , vo2peak may be expressed in normalized and absolute units,35 and the two different modalities may express complementary facets of aerobic capacity . it is possible that the use of different thresholds to stratify vo2peak values could yield different results regarding correlation and agreement . this may also explain the differences in agreement observed between the original bode and the mbode% compared with the mbode . however , in this study we used the same thresholds proposed by cardoso et al17 to ensure comparability . one difference between the present study and that of cardoso et al17 relies in the use of a cycle ergometer for vo2peak stratification instead of a treadmill . maximal oxygen uptake is reported to be , on average , 5%10% higher on a treadmill than on a cycle ergometer . since some abnormalities may occur only at the highest metabolic demand , the exact determination of vo2max in clinical practice is critical.14 it should be noted , however , that both methods have been validated for copd patients , with no clear difference between the two forms of exercise.36 in summary , our study suggests that the novel mbode indexes are highly correlated , but significantly different from each other ( mean difference of approximately 1 bode point ) as well as from the original bode index . taken together , our data reinforce the idea that the original bode index is useful in clinical characterization of copd patients .
aimit has been recently shown that the original bode index has a high degree of correlation with two modified bode indexes using maximal oxygen uptake expressed either as ml / min / kg ( mbode ) or as the percentage predicted ( mbode% ) . in this study we investigated the agreement between the two modified bode indexes ( mbode and mbode% ) in patients with stable chronic obstructive pulmonary disease ( copd).methodsa total of 169 patients with stable copd 
 were enrolled in this cross - sectional study . 
 differences between the two mbode indexes were assessed using kappa coefficients and bland - altman plots . 
 one out of every three patients underwent the six - minute walking test to investigate the agreement with the original bode index.resultscorrelations between the two mbode indexes with each other ( r = 0.96 , p < 0.001 ) and with the original bode index ( mbode r = 0.88 , p < 
 0.001 ; mbode% r = 0.93 , p < 
 0.001 ) were excellent . 
 however , the two mbode indexes were significantly different from each other ( mbode 5.27  2.3 versus mbode% 4.31  2.5 ; p < 0.001 ) . 
 the kappa coefficients were significantly lower ( entire study group k = 0.5 , p < 
 0.001 ) for every gold stage . 
 the mean difference between the two mbode indexes was 0.8  0.6 units . 
 differences with the original bode were higher for the mbode ( 1.8  0.9 ) than for the mbode% ( 0.6  0.8).conclusionsthe new mbode indexes are highly correlated but significantly different from each other . the differences between the novel indexes deserve further scrutiny .