when humans are exposed to cold , information from skin temperature sensors and blood temperature sensors in the hypothalamus activates the sympathetic nervous system , leading to skin vasoconstriction and reduced heat loss . the downside of this mechanism is that the blood flow in the fingers and toes may become so low that tissue integrity is at stake ( wilson and goldman 1970 ) . fortunately , at extremity skin temperatures below about 15c a sudden periodic vasodilatation is often observed , first described by lewis ( 1930 ) . the most likely mechanism for this vasodilatation is the muscular wall of the arterio - venous anastomoses in the fingers and toes which can not maintain vascular tone when cooled ( daanen 2003 ) . the opening of these vessels lead to increased blood flow and , indirectly , increased local skin temperatures . regardless of the underlying mechanisms , this cold induced vasodilatation ( civd ) response is often considered as a protective mechanism against local cold injuries ( daanen 2003 ; wilson and goldman 1970 ) . indeed , it has been shown that subjects with a reduced civd response have a higher risk of frostbite ( daanen and van der struijs 2005 ) . therefore , it may be desirable for subjects working in the cold to have a fast civd response of high magnitude , leading to research into how to manipulate and possibly enhance civd . the idea of using repeated local cold exposure as a training stimulus for increased civd comes from population cross - sectional studies . these studies showed that natives of circumpolar environments ( e.g. , inuits and sami ) exhibit a higher mean finger temperature compared to people who live in a more temperate climate when exposed to cold ( krog et al . an enhanced civd reaction was also observed in fishermen compared to a control group less regularly exposed to cold ( krog et al . nelms and soper ( 1962 ) found that fish filleters , who are exposed to local cold only , also showed a significant earlier civd reaction compared to the control group . however , the possibility of self - selection for these particular occupations should not be excluded . some studies found a lowered mean finger temperature after a 2-week arctic expedition , which may indicate a higher risk for cold injuries ( livingstone 1976 ) . on the other hand , purkayastha et al . ( 1992 ) observed higher finger temperatures in indian soldiers after a 7-week expedition to the arctic environment . in all reported lab studies on trainability , only either the hand or the foot was exposed to cold . three studies were identified in which the foot was trained ( reynolds et al . 2007 ; savourey 1996 ; yoshimura 1960 ) . ( 2007 ) observed no differences , the other two studies observed higher mean foot temperatures after repeated immersion . for hand immersion , most studies showed no training effect ( eagen 1966 ; geurts et al . 2006a ; glaser and whittow 1957 ; obrien 2005 ) , three studies showed a negative training effect ( daanen et al . 2007 ; geurts et al . 2005a ; mekjavic et al . 2008 ) and only two studies showed positive effects ( adams and smith 1962 ; geurts et al . , it seems that training of the foot is more successful than training of the hands or fingers . civd of the fingers is generally much more pronounced than civd of the toes ( cheung and mekjavic 2007 ; van der struijs et al . 2008 ) and there may be more scope for trainability in the toes than in the fingers . therefore , a study was performed in which the civd trainability was investigated for fingers and toes . this was done by tracking thermal responses of the fingers and toes over 15  days of repeated local immersion in 8c water . in line with the general picture from the existing literature , we hypothesized that toe civd would show more improvement than finger civd following repeated immersions . eighteen students volunteered for participation in this study . during the first day , two subjects indicated that they could not tolerate the cold water during immersion of the hands and feet and were withdrawn . the remaining 16 subjects , nine males and seven females , participated until the end . the subjects had a mean ( sd ) age of 20.4 ( 2.2 ) years , mean ( sd ) height of 177.7 ( 1.1 )  cm , and mean ( sd ) body mass of 71.5 ( 12.4 )  kg . one subject was left - handed , 15 were right - handed and all were non - smokers . all subjects were informed about the potential risks and discomforts of the study and gave their written informed consent to participate . all subjects were medically screened for cardiovascular diseases and raynauds syndrome . after entering the room ( ambient temperature 2024c , relative humidity 3070% ) , the subjects wore shoes , socks , trousers or shorts and a shirt ( one single layer of clothing ) . for the first 10  min , the subjects rested while the measurement equipment was attached to the body by the researcher . thermistors at the fingers were taped just lateral of the nail bed , since the reproducibility of skin temperature is better in the nail bed ( obrien 2005 ) . ( 2007 ) found a highly variable civd response at the nailbed of the toes . therefore we chose for the toe pad , which is the common position in most previous research , so that comparison to previous studies is enabled ( e.g. van der struijs et al . five minutes prior to immersion of the hand and foot in cold water , the subjects immersed their hand and foot in water of 3436c to create similar starting conditions for every subject . the hand was positioned at the level of the heart , while the foot was placed in a water tank located on the floor . the hand as well as the foot made no contact with the bottom of the water tanks , because of a water - permeable mesh ( 3mesh , muller textil gmbh , wiehl , germany ) that was lying at the bottom of the water tanks . after 5  min in warm water the subject put the right hand ( up to the ulnar and radial styloids ) and foot ( up to the malleolus ) for 30  min into cold stirred tap water of 8c . every 5  min , starting at minute five , tactile sensitivity of the tip of the index finger was tested with semmes  weinstein monofilaments . directly after the semmes  weinstein test , the subjects used the non - immersed hand to fill in a pain score for their hand as well as foot . after 30  min of cold immersion , the hand and foot were taken out of the water tanks and patted dry with a towel . day 1 was the pre - test and day 15 the post - test session . there were 2  days between the pre - test and the second immersion . between day 14 and the post - test , the left , non - acclimatized hand and foot were immersed at day 1 ( pre - test ) and 15 ( post - test ) as well , before or after ( counterbalanced ) the immersion of the right hand and foot . the hand immersion bath had a dimension of 49.5 ( l )   29  ( w )    13 ( h )  cm , and the foot water bath had a dimension of 46 ( l )    36  ( w )    22.5 ( h )  cm . to minimize the heat loss or heat gain of the water in the baths , they were insulated with polystyrene . the temperature was controlled within 1c using a thermostat bath ( tlc 15 , pm tamson instruments , bleiswijk , the netherlands ) . finger and toe temperatures were continuously monitored using thermistors ( type p-8432 , icbt , tokyo , japan ) attached to the skin by one layer of leukoplast tape ( bsn medical & gmbh & co.kg , d-22771 , hamburg , germany ) and connected to a mobi8 data acquisition system ( tms international bv , oldenzaal , the netherlands ) . the lowest value over the 30  min immersion interval was defined as the minimum temperature ( tmin ) . the mean ( tmean ) and maximum ( tmax ) temperatures were calculated over the 5- to 30-min interval . civd reactions were defined as a continuous rise of at least 1c . to exclude minor fluctuations , we averaged the values over a period 20  s before and 20  s after the measurement for all temperatures . when the rise was < 1c , the response was counted as  no civd , when it was 1c or more it was counted as a civd response . the onset time is the time in seconds from start of the immersion until the start of a continuous increase of temperature of at least 1c . pain was assessed every 5  min using a 010 visual analog scale ( vas ) numeric pain distress scale . tactile sensitivity at the tip of the index finger was assessed using semmes  weinstein monofilaments ( bell - krotoski and tomancik 1987 ) . the subjects turned the hand under water every 5  min for about 10  s to enable determination of tactile sensitivity . since most studies have limited statistical power , we included 16 subjects in the study . based on finger pad temperatures in the study of obrien ( 2005 ) , differences in onset time of 25  s and differences in peak temperature of 1.5c can be detected . we measured all fingers and toes because prevalence of a civd in a single finger or toe shows considerable variability ( cheung and mekjavic 2007 ; mekjavic et al . data for skin temperature , pain and tactile sensitivity were compared using a two - way ( condition    time ) factorial analysis of variance ( anova ) , with finger and toe temperatures statistically treated as independent . civd parameters between pre- and post - test were identified using a paired t test . differences in the number of civd reactions between pre- and post - test were analyzed non - parametrically . the results are expressed as means ( sd ) , and significance was accepted as p  <  0.05 . eighteen students volunteered for participation in this study . during the first day , two subjects indicated that they could not tolerate the cold water during immersion of the hands and feet and were withdrawn . the remaining 16 subjects , nine males and seven females , participated until the end . the subjects had a mean ( sd ) age of 20.4 ( 2.2 ) years , mean ( sd ) height of 177.7 ( 1.1 )  cm , and mean ( sd ) body mass of 71.5 ( 12.4 )  kg . one subject was left - handed , 15 were right - handed and all were non - smokers . all subjects were informed about the potential risks and discomforts of the study and gave their written informed consent to participate . after entering the room ( ambient temperature 2024c , relative humidity 3070% ) , the subject sat down on a chair . the subjects wore shoes , socks , trousers or shorts and a shirt ( one single layer of clothing ) . for the first 10  min , the subjects rested while the measurement equipment was attached to the body by the researcher . thermistors at the fingers were taped just lateral of the nail bed , since the reproducibility of skin temperature is better in the nail bed ( obrien 2005 ) . ( 2007 ) found a highly variable civd response at the nailbed of the toes . therefore we chose for the toe pad , which is the common position in most previous research , so that comparison to previous studies is enabled ( e.g. van der struijs et al . five minutes prior to immersion of the hand and foot in cold water , the subjects immersed their hand and foot in water of 3436c to create similar starting conditions for every subject . the hand was positioned at the level of the heart , while the foot was placed in a water tank located on the floor . the hand as well as the foot made no contact with the bottom of the water tanks , because of a water - permeable mesh ( 3mesh , muller textil gmbh , wiehl , germany ) that was lying at the bottom of the water tanks . after 5  min in warm water the subject put the right hand ( up to the ulnar and radial styloids ) and foot ( up to the malleolus ) for 30  min into cold stirred tap water of 8c . every 5  min , starting at minute five , tactile sensitivity of the tip of the index finger was tested with semmes  weinstein monofilaments . directly after the semmes  weinstein test , the subjects used the non - immersed hand to fill in a pain score for their hand as well as foot . after 30  min of cold immersion , the hand and foot were taken out of the water tanks and patted dry with a towel . day 1 was the pre - test and day 15 the post - test session . there were 2  days between the pre - test and the second immersion . between day 14 and the post - test , the left , non - acclimatized hand and foot were immersed at day 1 ( pre - test ) and 15 ( post - test ) as well , before or after ( counterbalanced ) the immersion of the right hand and foot . the hand immersion bath had a dimension of 49.5 ( l )   29  ( w )    13 ( h )  cm , and the foot water bath had a dimension of 46 ( l )    36  ( w )    22.5 ( h )  cm . to minimize the heat loss or heat gain of the water in the baths , they were insulated with polystyrene . the temperature was controlled within 1c using a thermostat bath ( tlc 15 , pm tamson instruments , bleiswijk , the netherlands ) . finger and toe temperatures were continuously monitored using thermistors ( type p-8432 , icbt , tokyo , japan ) attached to the skin by one layer of leukoplast tape ( bsn medical & gmbh & co.kg , d-22771 , hamburg , germany ) and connected to a mobi8 data acquisition system ( tms international bv , oldenzaal , the netherlands ) . the lowest value over the 30  min immersion interval was defined as the minimum temperature ( tmin ) . the mean ( tmean ) and maximum ( tmax ) temperatures were calculated over the 5- to 30-min interval . civd reactions were defined as a continuous rise of at least 1c . to exclude minor fluctuations , we averaged the values over a period 20  s before and 20  s after the measurement for all temperatures . when the rise was < 1c , the response was counted as  no civd , when it was 1c or more it was counted as a civd response . the onset time is the time in seconds from start of the immersion until the start of a continuous increase of temperature of at least 1c . pain was assessed every 5  min using a 010 visual analog scale ( vas ) numeric pain distress scale . tactile sensitivity at the tip of the index finger was assessed using semmes  weinstein monofilaments ( bell - krotoski and tomancik 1987 ) . the subjects turned the hand under water every 5  min for about 10  s to enable determination of tactile sensitivity . since most studies have limited statistical power , we included 16 subjects in the study . based on finger pad temperatures in the study of obrien ( 2005 ) , differences in onset time of 25  s and differences in peak temperature of 1.5c can be detected . we measured all fingers and toes because prevalence of a civd in a single finger or toe shows considerable variability ( cheung and mekjavic 2007 ; mekjavic et al . data for skin temperature , pain and tactile sensitivity were compared using a two - way ( condition    time ) factorial analysis of variance ( anova ) , with finger and toe temperatures statistically treated as independent . civd parameters between pre- and post - test were identified using a paired t test . differences in the number of civd reactions between pre- and post - test were analyzed non - parametrically . the results are expressed as means ( sd ) , and significance was accepted as p  <  0.05 . the finger and toe skin temperatures averaged over all fingers and toes during the days of immersion are shown in fig .  1 . finger skin temperature essentially remains constant ; toe skin temperature shows an increase . during the last 2  days a drop in toe and finger skin temperature was noticed.fig . 1finger and toe skin temperatures ( c ) averaged over minutes 530 and over all digits during immersion in 8c water for 15 consecutive days . all values are in c finger and toe skin temperatures ( c ) averaged over minutes 530 and over all digits during immersion in 8c water for 15 consecutive days . all values are in c cold induced vasodilation parameters of the pre- and post - test are shown in table  1 . due to technical problems with the attachment of the sensors to the skin , there were several drop - outs randomly divided over the subjects during the first day . while the untrained extremities did not change in temperature after 15  days of immersion , the trained extremities did change . the number of civd reactions with at least one wave dropped significantly in trained fingers after 15  days of cold water immersion from 52 to 24% ( p <  0.01 ) while it remained unchanged in the untrained fingers ( 39 vs. 46% ) . the onset times of civd were widely spread and did not display significant differences ; neither did the peak of the civd wave . while the trained fingers showed a significant decrease in temperature ( for tmean , tmin and tmax , p  <  0.001 ) during the post - test , the trained toes showed an increase in temperature during the post - test ( tmean and tmin , p  <  0.05 ; tmax , p  <  0.01 , ) . finger skin temperatures were significantly lower for the thumb ( p  <  0.05 ) , index finger ( p  <  0.05 ) , middle finger ( p  <  0.05 ) , ring finger ( p  <  0.01 ) and little finger ( p  <  0.001 ) during day 15 as compared to day 1 . toe temperatures were higher for the middle ( p  <  0.05 ) , fourth ( p  <  0.01 ) and little toe ( p  <  0.05 ) and not significantly different between day 1 and day 15 for the big toe and the index toe.table  1comparison between pre- and post - test for civd parameterspre - testpost - testfingers toesfingers toestraineduntrainedtraineduntrainedtraineduntrainedtraineduntrainedn7364636879807979#civd38  =  52%25  =  39%28  =  44%30 =  44%19  =  24%37  =  46%23  =  29%30  =  38%onset time ( s)676 ( 462)629 ( 456)766 ( 292)763 ( 349)716 ( 407)629 ( 471)735 ( 338)741 ( 419)tpeak ( c)10.54 ( 0.69)11.88 ( 2.07)11.17 ( 0.99)12.64 ( 1.81)10.27 ( 0.79)11.48 ( 1.90)11.85 ( 2.05)12.60 ( 2.35)tmean ( c)9.28 ( 0.54)9.41 ( 1.03)9.49 ( 0.89)9.73 ( 1.04)8.91 ( 0.44)*9.55 ( 1.10)10.03 ( 1.38)9.96 ( 1.23)tmin ( c)8.69 ( 0.32)8.69 ( 0.41)8.81 ( 0.72)8.88 ( 0.70)8.48 ( 0.24)*8.72 ( 0.45)9.15 ( 0.89)8.97 ( 0.78)tmax ( c)10.21 ( 0.91)10.49 ( 1.89)10.85 ( 1.23)11.54 ( 1.76)9.63 ( 0.86)*10.72 ( 2.13)11.68 ( 2.16)11.92 ( 2.23)n is the number of experimental datasets . the maximum is 16 subjects    5  toes / fingers  =  80 . mainly in the pre - test , several measurements had to be disregarded since the sensors became detached from the skin . # civd reactions stand for the number of experimental datasets with at least one civd reaction . values are mean ( sd ) *  significantly different from pre - test values ( p  <  0.001)significantly different from pre - test values ( p  <  0.01)significantly different from pre - test values ( p  <  0.05 ) comparison between pre- and post - test for civd parameters the maximum is 16 subjects    5  toes / fingers  =  80 . mainly in the pre - test , several measurements had to be disregarded since the sensors became detached from the skin . # civd reactions stand for the number of experimental datasets with at least one civd reaction . values are mean ( sd ) *  significantly different from pre - test values ( p  <  0.001 ) significantly different from pre - test values ( p  <  0.01 ) significantly different from pre - test values ( p  <  0.05 ) pain in the trained hand and foot decreased over time , as can be clearly seen in fig . this graph displays the average pain experienced during cold water immersion each of the 15  days . pain in the hand dropped from 4.5 ( 1.8 ) at day 1 to 2.9 ( 1.9 ) at day 15 ( p  <  0.001 ) . pain in the foot dropped from 4.6 ( 1.7 ) to 2.3 ( 1.8 ) ( p  <  0.001 ) . 2average pain score and standard error of the mean of the trained hand and foot during immersion over 15  days . note that at day 1 and 15 the immersion sequence of the extremities was counterbalanced average pain score and standard error of the mean of the trained hand and foot during immersion over 15  days . note that at day 1 and 15 the immersion sequence of the extremities was counterbalanced pain was significantly less during post - test compared to the pre - test ( table  2 ) . this is true for hand as well as foot and trained side as well as the untrained side . because the scores of the trained and untrained hand at day 1 were equal ( no significant change between these scores ) , the comparison between the scores at day 15 show significantly less pain in the trained hand , compared to the untrained hand at day 15 . the same is true for foot , but a note has to be made here , because pain in the trained foot at day 1 was already significantly lower compared to the pain in the untrained foot ( p  <  0.05).table  2pre- ( day 1 ) and post - test ( day 15 ) pain scores ( sd)painday 1day 15trained hand4.5 ( 1.8)2.9 ( 1.9)*untrained hand4.8 ( 1.8)3.9 ( 2.2)*trained foot4.6 ( 1.7)2.3 ( 1.8)*untrained foot5.1 ( 1.7)4.2 ( 2.1 ) * *  significantly different from day 1 values ( p  <  0.001 ) pre- ( day 1 ) and post - test ( day 15 ) pain scores ( sd ) *  significantly different from day 1 values ( p  <  0.001 ) tactile sensitivity of the index finger did not change significantly over 15  days , but was related to the skin temperature of the index finger . figure  3 shows the relationship between index finger skin temperature and tactile sensitivity . for a mean ( sd ) skin temperature of 8.78c ( 0.35 ) only a thick filament ( 4.08  g ) could be detected , whereas for an index finger skin temperature of 10.35c ( 1.83 ) even thin filaments of 2.44  g could be detected.fig . data for this figure included all measurements over the 15  days of temperature and sensitivity ( trained as well as untrained index finger ) . vertical bars denote the standard error of the mean relation between index finger skin temperature and tactile sensitivity . data for this figure included all measurements over the 15  days of temperature and sensitivity ( trained as well as untrained index finger ) . the main objective of this study was to investigate the effect of repeated local cold exposure on thermal responses in both the fingers and toes . furthermore , we looked at the influence of local cold acclimation to pain and tactile sensitivity . fifteen days of immersing the right hand and foot simultaneously in cold water for 30  min produced somewhat contradictory responses in the fingers versus the toes . namely , the incidences of civd in toes pre- and post - acclimation were not different , from 44% of the possible events pre - acclimation to 29% post - acclimation . however , the tmean , tmin , and tmax increased post - acclimation , especially in the third , fourth , and fifth toes ; although significant , the magnitude of the changes in our study is small , at < 1c on average . our data , combined with the positive training effect for toes observed by yoshimura ( 1960 ) and savourey ( 1996 ) , seem to suggest that toe blood flow is trainable , albeit modestly . to date , only reynolds et al . in contrast , the fingers proved unresponsive to repeated cold exposures , and indeed the thermal response may have degraded . the number of civd reactions in the fingers dropped significantly from 54 to 24% , post - acclimation , as did the finger skin temperature parameters . less subjects showed civd post - acclimation and the subjects that still had civd showed it to a lesser extent . results in the literature are conflicting , but the majority of studies observe no effect or a detrimental effect of repeated cold exposure to finger skin temperature . the decrease of civd reactions and temperature in fingers are in line with previous findings by mekjavic et al . ( 2008 ) , who found similar results of these parameters after 13  days of right hand immersion in water of 8c for 30  min and geurts et al . ( 2005b ) , who reported a drop of mean finger skin temperature after investigating the civd response with ten subjects in similar conditions as used in our study . 2006a ; glaser and whittow 1957 ; obrien 2005 ) , while only two studies observed positive effects ( adams and smith 1962 ; geurts et al . therefore , the risk for cold injuries in the fingers may be elevated in individuals acclimated to cold . this is further supported by a decrease in pain sensations with both hand and feet cooling after acclimation . the decrease in finger skin temperature due to training this drop in finger and toe temperature during the last 2  days ( fig . 1 ) may be related to the ambient temperature outside of the building that was about 16c during days 14 and 15 and 1822c in the preceding days ( meteorological data from schiphol airport that is in close vicinity to the measurement location ) . the lower ambient temperature may have caused a reduction in the thermal state of the body prior to the experiment . controlling the temperature of the experimental room and immersing the extremities in warm water ( 35c ) for 5  min prior to the cold water immersion with the intention to create a stable thermal condition for the subjects may have been insufficient to eliminate the effect of the external ambient temperature on the thermal status of the body . on the other hand , it may simply be that the changes during the last 2  days reflect real changes due to cold training that may only take effect following prolonged and repeated exposures . the ambient temperature was identical at day 1 and day 15 ( 16c ) ; therefore , a fair comparison can be made between the pre- and post - test . the decrease in finger skin temperature and increase in toe skin temperature during the civd training period of 15  days may be partly attributed to anatomical differences . the feet have a smaller surface area to volume ratio than the hands ( 68 vs. 104  m for men ( tikuisis et al . 2001 ) , which leads to slower cooling of the feet as compared to the hands . this may be the reason that civd is less pronounced in the feet than in the hands ( van der struijs et al . therefore , it may be more effective to enhance vasoconstrictory tone and the sensitivity of local blood vessels for cold in the fingers as opposed to the toes to maintain the overall thermal status of the body . another explanation may be that since the toes are more prone to cold injuries than fingers ( daanen and van der struijs 2005 ) , it is more important to maintain tissue integrity in the toes and thus enhance local blood flow there . the observed changes in finger and toe temperatures after 15  days of cold water immersion in our study are not likely to be of central origin . when sympathetic outflow would have increased , both fingers and toes would have shown vasoconstriction . therefore , a peripheral mechanism should be held responsible for the observed changes , such as an increased sensitivity to cold at the fingers leading to vasoconstriction and a decreased sensitivity to cold at the toes leading to a reduction in vasoconstrictory tone . this is true for both feet and hands and for the trained and untrained side . figure  2 clearly shows that the greatest decline in pain occurs in the first few days , independently of possible adaptations in skin temperature . the relative increase in pain during days 8 and 12 may be explained by the fact that these measurement days were relatively often on mondays , and the 2-day weekend break from cold exposure may have reduced pain resistance . because pain can be seen as a warning signal of the body for extreme cold , some researchers claim that a decrease in finger temperature , in combination with a decrease in pain due to training , lead to a habituation effect ( geurts et al . when people experience less pain after a few days of repeated cold exposure it is not expected that they adjust their behavior to their real thermal status , which increases the risk of cold injuries . previous studies reported that tactile sensitivity was unaffected when measured before and after repeated cold exposure ( geurts et al . was measured while the hand remained immersed in cold water , such that no rewarming due to removal from the cold exposure occurred . our results clearly show that a lower skin temperature leads to a decreased tactile sensitivity . this is in line with a deterioration of tactile sensitivity as a result of decreased skin temperature found by gescheider et al . for the untrained extremities no significant differences were detected in our study , so it can be concluded that the observed differences in finger and toe skin temperature must reflect local effects of repeated immersion . if there would have been a change in central drive due to repeated immersion , changes in both the trained and untrained extremities should have been observed . sympathetic activity is the central drive that is generally considered to play a major role in the control of civd ( daanen 2003 ) . the sympathetic nerves innervate the strong muscular wall of the arterio - venous anastomoses that are abundantly present at civd locations . sympathetic activation thus leads to vasoconstriction and a reduced civd response ( daanen 2003 ) . the absence of any change in the non - trained fingers and toes indicate that the sympathetic activity is probably unaltered due to training . in summary , sixteen subjects simultaneously exposed a hand and foot to 8c water for 30  min daily for a period of 15 consecutive days excluding weekends . the toe temperatures showed a small increase after 15  days , while the finger temperatures dropped , as well as the number of civd responses in the finger . therefore , the risk for cold injuries may increase in trained fingers , also because pain sensation gradually decreased so that the warning system becomes less alert . we , therefore , do not recommend repeated cold water immersion of the hands as a method for preventing cold injuries .
subjects that repeatedly have to expose the extremities to cold may benefit from a high peripheral temperature to maintain dexterity and tissue integrity . 
 therefore , we investigated if repeated immersions of a hand and a foot in cold water resulted in increased skin temperatures . 
 nine male and seven female subjects ( mean 20.4 ; sd 2.2  years ) immersed their right ( trained ) hand and foot simultaneously in 8c water , 30  min daily for 15  days . during the pre and post - test ( days 1 and 15 , respectively ) 
 the left ( untrained ) hand and foot were immersed as well . 
 pain , tactile sensitivity and skin temperatures were measured every day . 
 mean ( sd ) toe temperature of the trained foot increased from 9.49c ( 0.89 ) to 10.03c ( 1.38 ) ( p  <  0.05 ) . 
 the trained hand , however , showed a drop in mean finger temperature from 9.28c ( 0.54 ) to 8.91c ( 0.44 ) ( p  <  0.001 ) and the number of cold induced vasodilation ( civd ) reactions decreased from 52% during the first test to 24% during the last test . 
 no significant differences occurred in the untrained extremities . 
 pain diminished over time and tactile sensitivity decreased with skin temperature . 
 the combination of less civd responses in the fingers after training , reduced finger skin temperatures in subjects that did show civd and the reduced pain and tactile sensitivity over time may lead to an increased risk for finger cold injuries . 
 it is concluded that repeated cold exposure of the fingers does not lead to favorable adaptations , but may instead increase the injury risk .