successful single - cell isolation is a primary step for subsequent 
 chemical and biological analyses of single cells . 
 conventional single - cell 
 isolation methods often encounter operational complexity , limited 
 efficiency , deterioration of cell viability , incompetence in the isolation 
 of a single - cell into nanoliter liquid , and/or inability to select 
 single adherent cells with specific phenotypes . here 
 , we develop a 
 hand - held single - cell pipet ( hscp ) that is rapid , operationally simple , 
 highly efficient , and inexpensive for unbiased isolation of single 
 viable suspended cells directly from submicroliter cell suspensions 
 into nanoliter droplets without the assistance of any additional equipment . 
 an integrated scp ( iscp ) has also been developed for selective isolation 
 of single suspended and adherent cells according to the fluorescence 
 imaging and morphological features . 
 the isolated single cells can 
 be conveniently transferred into standard 96-/384-well plates , petri 
 dishes , or vials for cloning , pcr , and other single - cell biochemical 
 assays .