glioma is the common and fatal primary brain tumor in adults and accounts for 44.69% of intracranial tumors . the current therapies for glioma , especially for high - grade glioma , are unsatisfactory because of its infiltrating growth . the prognosis of glioma is still poor even when glioma is completely resected , or treated in combination with radiation and chemotherapy after surgery . therefore , further understanding of the molecular pathological mechanism of glioma may provide new ideas to improve the prognosis and therapy of glioma . located on the chromosomal region 5pl3.3 , golph3 encodes a protein of 34 kd named gpp34/gmx33/mldas . golph3 is a member of trans - golgi network ( tgn ) protein family , and affects the secretion of golgi apparatus . proteins synthesized in the endoplasmic reticulum enter the cis - golgi network in the form of vesicles . after processing and modification , these proteins get out of trans - golgi network through a budding process as secretory proteins . through participating in protein processing , classification , packaging , and transportation in the golgi apparatus , golph3 may play a part in tumor occurrence and progression . in addition , golph3 is required for contractile ring formation and membrane trafficking during cytokinesis . several studies have confirmed that golph3 is overexpressed in breast cancer , ovarian cancer , renal cancer , non - small cell lung cancer , and gastric cancer . n - myc downstream regulated gene 1 ( ndrg1 ) was first isolated as a gene upregulated in n - myc knockout mouse embryos , and its expression was repressed by n - myc and c - myc . ndrg1 is widely expressed in normal tissues but was downregulated in primary and metastatic cancers , including colon , prostate , breast , esophageal squamous cancer , pancreatic cancer , and glioma . in this context ndrg1 has been implicated in many cellular processes including cell cycle progression , apoptosis , differentiation , and vesicular transport . nevertheless , the relationship between golph3 and ndrg1 in tumor development is unclear . the present study aimed to explore the molecular mechanism by which golph3 promotes glioma . we further analyzed the role of ndrg1 and golph3 in the apoptosis of glioma cells , and examined the expression of golph3 and ndrg1 in glioma samples . the human glioma cell lines u87 and u251 were purchased from shanghai cell bank , type culture collection committee , chinese academy of sciences . all of the cells were cultured in dulbecco s modified eagle s medium supplemented with 10% fetal bovine serum ( hyclone ) . sirna oligos for golph3 ( cat # : sc-91952 , santa cruz biotechnology ) and ndrg1 ( cat # : sc-36021 , santa cruz biotechnology ) or negative control scramble sirnas were transfected into glioma cells using lipofectamine 2000 ( invitrogen ) according to the manufacturer s instructions . one day prior to transfection , 210 cells were plated in 6-well plates and grown to 3050% confluence . 100 pmol of sirna duplex and 5 l of lipofectamine 2000 were mixed and incubated at room temperature for 20 min , and then added into the cells . cells were lysed by lysis buffer , and bca protein assay kit ( thermo fisher scientific ) was used to measure protein concentrations . equal amounts of proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis ( sds  page ) , transferred to polyvinylidene difluoride ( pvdf ) membrane , blocked with 3% bovine serum albumin ( bsa ) , and incubated with specific antibodies against golph3 ( 1 : 1000 , abcam , uk ) , cleaved caspase 3 ( 1 : 200 , santa cruz , usa ) , -actin ( 1 : 3000 , zhongshan , china ) , and ndrg1 ( 1 : 1000 , abcam , uk ) at 4c overnight . the blots were developed after incubation with peroxidase - conjugated secondary antibodies ( 1 : 4000 ) at room temperature for 1 h and then were imaged with a chemiluminescence detection kit ( pierce , usa ) . the densitometry value of blots was normalized by the internal loading control to determine relative protein levels . apoptosis was detected using the fitc annexin v apoptosis detection kit ( bd biosciences ) according to the manufacturer s instructions . briefly , u251 and u87 cells were washed twice with cold phosphate - buffered saline ( pbs ) and then resuspended in 1x binding buffer at a concentration of 110 cells / ml . next , 100 l of solution ( 110 cells ) was incubated with 5 l of fitc annexin v and 5 l of pi for 15 min at 25c in the dark . cells that are considered viable are fitc annexin v and pi negative ; cells that are in early apoptosis are fitc annexin v positive and pi negative ; and cells that are in late apoptosis or already dead are both fitc annexin v and pi positive . paraffin - embedded pathological sections including 6 non - tumor brain tissues from controls , 9 grade ii glioma tissues , 10 grade iii glioma tissues , and 15 grade iv glioma tissues . normal cerebral tissues were acquired from cerebral trauma patients undergoing internal decompression surgery . for the use of these clinical materials for research purposes , prior consent of the patients and approval from the institutional research ethics committee the s - p immunohistochemistry kit ( bioss , china ) was used for immunohistochemical staining . tissue blocks were sectioned at a thickness of 4 m , dewaxed in xylene , and then hydrated in 85% ethanol . next , the tissues were incubated in 3% hydrogen peroxide for 5 minutes to block endogenous peroxidase and then blocked with 10% goat serum . the sections were then incubated with golph3 ( 1 : 200 , abcam , uk ) or ndrg ( 1 : 400 , abcam , uk ) antibody overnight at 4c . the tissue sections were then incubated with biotinylated secondary antibody ( bioss , china ) at room temperature for 1 hour , followed by incubation with horseradish peroxidase ( hrp)-conjugated goat anti - mouse igg ( bioss , china ) for 30 minutes . all slides were interpreted by two experienced and independent pathologists who were unaware of the clinical data . for each slide , 5 visual fields were randomly selected and examined with a microscope to assess the staining intensity . the following scoring system was used for the staining results : negative ( 0 ) ; weak ( 1 ) ; moderate ( 2 ) ; and strong ( 3 ) . the number of stained cells and the total number of cells in each visual field were also counted , and the ratio between the stained and total cells was calculated . the following scoring system was used for the stained cell ratio : < 5% ( 0 ) ; 625% ( 1 ) ; 2650% ( 2 ) ; and > 50% ( 3 ) . we multiplied the scores from the two systems on the same tissue sample , and the following definitions were used : negative (  ) if the resulting score was 0 ; weak positive ( + ) if the resulting score was 13 ; strong positive ( + + ) if the resulting score was 46 ; and very strong positive ( + + + ) if the resulting score was 79 . the numbers of positive cells were counted from 5 randomly selected fields , and the mean number of positive cells in each field was calculated . the study protocol was approved by the ethics committee of liaocheng people s hospital , and written informed consent was obtained from each patient . comparisons between groups for statistical significance were carried out with a 2-tailed paired student s t test . the human glioma cell lines u87 and u251 were purchased from shanghai cell bank , type culture collection committee , chinese academy of sciences . all of the cells were cultured in dulbecco s modified eagle s medium supplemented with 10% fetal bovine serum ( hyclone ) . sirna oligos for golph3 ( cat # : sc-91952 , santa cruz biotechnology ) and ndrg1 ( cat # : sc-36021 , santa cruz biotechnology ) or negative control scramble sirnas were transfected into glioma cells using lipofectamine 2000 ( invitrogen ) according to the manufacturer s instructions . one day prior to transfection , 210 cells were plated in 6-well plates and grown to 3050% confluence . 100 pmol of sirna duplex and 5 l of lipofectamine 2000 were mixed and incubated at room temperature for 20 min , and then added into the cells . cells were lysed by lysis buffer , and bca protein assay kit ( thermo fisher scientific ) was used to measure protein concentrations . equal amounts of proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis ( sds  page ) , transferred to polyvinylidene difluoride ( pvdf ) membrane , blocked with 3% bovine serum albumin ( bsa ) , and incubated with specific antibodies against golph3 ( 1 : 1000 , abcam , uk ) , cleaved caspase 3 ( 1 : 200 , santa cruz , usa ) , -actin ( 1 : 3000 , zhongshan , china ) , and ndrg1 ( 1 : 1000 , abcam , uk ) at 4c overnight . the blots were developed after incubation with peroxidase - conjugated secondary antibodies ( 1 : 4000 ) at room temperature for 1 h and then were imaged with a chemiluminescence detection kit ( pierce , usa ) . the densitometry value of blots was normalized by the internal loading control to determine relative protein levels . apoptosis was detected using the fitc annexin v apoptosis detection kit ( bd biosciences ) according to the manufacturer s instructions . briefly , u251 and u87 cells were washed twice with cold phosphate - buffered saline ( pbs ) and then resuspended in 1x binding buffer at a concentration of 110 cells / ml . next , 100 l of solution ( 110 cells ) was incubated with 5 l of fitc annexin v and 5 l of pi for 15 min at 25c in the dark . cells that are considered viable are fitc annexin v and pi negative ; cells that are in early apoptosis are fitc annexin v positive and pi negative ; and cells that are in late apoptosis or already dead are both fitc annexin v and pi positive . paraffin - embedded pathological sections including 6 non - tumor brain tissues from controls , 9 grade ii glioma tissues , 10 grade iii glioma tissues , and 15 grade iv glioma tissues . normal cerebral tissues were acquired from cerebral trauma patients undergoing internal decompression surgery . for the use of these clinical materials for research purposes , prior consent of the patients and approval from the institutional research ethics committee the s - p immunohistochemistry kit ( bioss , china ) was used for immunohistochemical staining . tissue blocks were sectioned at a thickness of 4 m , dewaxed in xylene , and then hydrated in 85% ethanol . next , the tissues were incubated in 3% hydrogen peroxide for 5 minutes to block endogenous peroxidase and then blocked with 10% goat serum . the sections were then incubated with golph3 ( 1 : 200 , abcam , uk ) or ndrg ( 1 : 400 , abcam , uk ) antibody overnight at 4c . the tissue sections were then incubated with biotinylated secondary antibody ( bioss , china ) at room temperature for 1 hour , followed by incubation with horseradish peroxidase ( hrp)-conjugated goat anti - mouse igg ( bioss , china ) for 30 minutes . all slides were interpreted by two experienced and independent pathologists who were unaware of the clinical data . for each slide , 5 visual fields were randomly selected and examined with a microscope to assess the staining intensity . the following scoring system was used for the staining results : negative ( 0 ) ; weak ( 1 ) ; moderate ( 2 ) ; and strong ( 3 ) . the number of stained cells and the total number of cells in each visual field were also counted , and the ratio between the stained and total cells was calculated . the following scoring system was used for the stained cell ratio : < 5% ( 0 ) ; 625% ( 1 ) ; 2650% ( 2 ) ; and > 50% ( 3 ) . we multiplied the scores from the two systems on the same tissue sample , and the following definitions were used : negative (  ) if the resulting score was 0 ; weak positive ( + ) if the resulting score was 13 ; strong positive ( + + ) if the resulting score was 46 ; and very strong positive ( + + + ) if the resulting score was 79 . the numbers of positive cells were counted from 5 randomly selected fields , and the mean number of positive cells in each field was calculated . the study protocol was approved by the ethics committee of liaocheng people s hospital , and written informed consent was obtained from each patient . comparisons between groups for statistical significance were carried out with a 2-tailed paired student s t test . the relationship between golph3 and ndrg1 expression was analyzed by the  test . to explore the role of golph3 in u251 and u87 cells apoptosis , we use sirna to knock down golph3 expression in glioma cells . forty - eight hours after transfection , the knockdown efficacy of golph3 sirnas was examined by western blot analysis . the results showed that golph3 sirnas effectively suppressed the expression of golph3 by 5060% in u251 cells and 40% in u87 cells ( figure 1a , 1b ) . the results showed that golph3 sirna - transfected u251 cells and u87 cells showed higher rate of apoptosis compared to the cells transfected with scrambled control ( figure 1c , 1d ) . the knockdown efficacy of ndrg1 sirnas was determined by western blot analysis , and we found that ndrg1 sirnas effectively suppressed the expression of ndrg1 by 55% in u251 cells and 45% in u87 cells ( figure 2a , 2b ) . the results showed that ndrg1 sirna - transfected u251 cells and u87 cells showed a lower rate of apoptosis compared to the cells transfected with scrambled control ( figure 2c , 2d ) . these data demonstrated that ndrg1 knockdown inhibited glioma cell apoptosis , contrary to the effect of golph3 knockdown . they all had three replicates in western blot and flow cytometry . to further investigate the relationship between golph3 , ndrg1 , and the apoptosis of glioma cells , we employed golph3 sirna to knock down golph3 in u251 cells . western blot analysis showed that the level of ndrg1 was increased by about 55% and the level of cleaved caspase 3 was increased by about 80% ( figure 3a , 3b ) . the results were consistent with the previous observation that golph3 knockdown promoted glioma cell apoptosis . next we found that ndrg1 knockdown had no significant effect on golph3 expression , but led to a decreased level of cleaved caspase 3 ( figure 3c , 3d ) . the results were consistent with the previous observation that ndrg1 knockdown inhibited the apoptosis of glioma cells . these data indicated that golph3 increased the cleavage of caspase 3 and apoptosis of glioma cells partially via downregulating ndrg1 . to confirm the opposite roles of golph3 and ndrg1 in the progression of glioma , we detected golph3 and ndrg1 expression in clinical glioma samples . western blot analysis showed that the protein level of golph3 increased with the advancement of glioma grade , while the protein level of ndrg1 decreased with the advancement of glioma grade ( figure 4a , 4b ) . furthermore , the distribution and expression of ndrg1 and golph3 in glioma samples were assessed by immunohistochemistry ( figure 4c ) . we found that higher glioma grade was correlated with a higher percentage of golph3-positive cells and a lower percentage of ndrg1-positive cells . the relative staining intensity was as follows : ndrg1 593.8% for grade ii , 332.9% for grade iii , and 112.9% for grade iv ; golph3 324.4% for grade ii , 493.5% for grade iii , and 794.8% for grade iv . notably , golph3 expression and ndrg1 expression in glioma samples were negatively correlated ( r=0.837 ; p<0.01 ) . collectively , these data suggested that golph3 and ndrg1 play opposite roles in the growth of human glioma . to explore the role of golph3 in u251 and u87 cells apoptosis , we use sirna to knock down golph3 expression in glioma cells . forty - eight hours after transfection , the knockdown efficacy of golph3 sirnas was examined by western blot analysis . the results showed that golph3 sirnas effectively suppressed the expression of golph3 by 5060% in u251 cells and 40% in u87 cells ( figure 1a , 1b ) . the results showed that golph3 sirna - transfected u251 cells and u87 cells showed higher rate of apoptosis compared to the cells transfected with scrambled control ( figure 1c , 1d ) . the knockdown efficacy of ndrg1 sirnas was determined by western blot analysis , and we found that ndrg1 sirnas effectively suppressed the expression of ndrg1 by 55% in u251 cells and 45% in u87 cells ( figure 2a , 2b ) . the results showed that ndrg1 sirna - transfected u251 cells and u87 cells showed a lower rate of apoptosis compared to the cells transfected with scrambled control ( figure 2c , 2d ) . these data demonstrated that ndrg1 knockdown inhibited glioma cell apoptosis , contrary to the effect of golph3 knockdown . to further investigate the relationship between golph3 , ndrg1 , and the apoptosis of glioma cells , we employed golph3 sirna to knock down golph3 in u251 cells . western blot analysis showed that the level of ndrg1 was increased by about 55% and the level of cleaved caspase 3 was increased by about 80% ( figure 3a , 3b ) . the results were consistent with the previous observation that golph3 knockdown promoted glioma cell apoptosis . next we found that ndrg1 knockdown had no significant effect on golph3 expression , but led to a decreased level of cleaved caspase 3 ( figure 3c , 3d ) . the results were consistent with the previous observation that ndrg1 knockdown inhibited the apoptosis of glioma cells . these data indicated that golph3 increased the cleavage of caspase 3 and apoptosis of glioma cells partially via downregulating ndrg1 . to confirm the opposite roles of golph3 and ndrg1 in the progression of glioma , we detected golph3 and ndrg1 expression in clinical glioma samples . western blot analysis showed that the protein level of golph3 increased with the advancement of glioma grade , while the protein level of ndrg1 decreased with the advancement of glioma grade ( figure 4a , 4b ) . furthermore , the distribution and expression of ndrg1 and golph3 in glioma samples were assessed by immunohistochemistry ( figure 4c ) . we found that higher glioma grade was correlated with a higher percentage of golph3-positive cells and a lower percentage of ndrg1-positive cells . the relative staining intensity was as follows : ndrg1 593.8% for grade ii , 332.9% for grade iii , and 112.9% for grade iv ; golph3 324.4% for grade ii , 493.5% for grade iii , and 794.8% for grade iv . notably , golph3 expression and ndrg1 expression in glioma samples were negatively correlated ( r=0.837 ; p<0.01 ) . collectively , these data suggested that golph3 and ndrg1 play opposite roles in the growth of human glioma . in this research , we demonstrated that knockdown of golph3 promoted the apoptosis of glioma cells and increased the level of cleaved caspase 3 . in contrast , knockdown of ndrg1 inhibited the apoptosis of glioma cells and reduced the level of cleaved caspase 3 . we further revealed the relationship between ndrg1 and golph3 and found that golph3 knockdown increased the expression of ndrg1 , but knockdown of ndrg1 had no obvious effect on golph3 expression . furthermore , we found a negative correlation of ndrg1 and golph3 expression in human glioma tissues . thus , golph3 may inhibit the apoptosis of glioma cells and promote glioma growth by downregulating ndrg1 . scott et al . first claimed that golph3 was a novel cellular oncogene that was frequently amplified in multiple solid tumors . further studies confirmed that golph3 plays an important role in the progression of various cancers . our previous work confirmed that golph3 expression was high in glioma and that the expression level increased with the increased degree of malignancy of the glioma . an additional study reported that overexpression of golph3 in glioblastoma was associated with worse prognosis . however , the mechanism underlying the potential oncogenic role of golph3 in glioma is not clear . it was reported that golph3 could regulate glioma cell invasion and migration through the golph3-mtor - yb1 or golph3-rhoa signaling pathway [ 2022 ] . in addition , the overexpression of golph3 promoted the proliferation of glioma cells through the pkd2-golph3-akt signaling pathway . thus , golh3 may become a novel therapeutic target of glioma . in this research we provided the first evidence that golph3 inhibited the apoptosis of glioma cells , and further explored the possible mechanism . ndrg1 is a differentiation related gene that has been proposed as a tumor suppressor in several cancers , including pancreatic cancer , prostate cancer , breast cancer , colorectal cancer , and glioma [ 2426 ] . in prostate and breast cancer , loss of ndrg1 expression has been consistently linked to tumor progression and metastasis as well as poor survival of cancer patients . interestingly , it has been reported that ndrg1 is necessary for p53-dependent apoptosis in colorectal adenocarcinoma cells . in addition , indolic diet derivative 3,3-diindolylmethane induced apoptosis in human colon cancer cells through upregulating ndrg1 . these results clearly demonstrate the role of ndrg1 in inducing the apoptosis of tumor cells . consistent with these data , in this study we found that ndrg1 knockdown led to decreased cleavage of caspase 3 and decreased apoptosis of glioma cells . sun et al . found that the mrna and protein expression of ndrg1 was decreased with the rise of tumor grade in 168 cases of glioma . thus , we further investigated the correlation of golph3 and ndrg1 in glioma samples . with the increase of malignancy of glioma tissue , the expression of golph3 in addition , we found that golph3 expression and ndrg1 expression were negatively correlated in clinical human glioma tissues . in u251 cells , we found that golph3 could regulate ndrg1 expression , but ndrg1 could not regulate golph3 expression , indicating that golph3 may act upstream of ndrg1 . the potential role of golph3 in trafficking is further supported by its direct , tight , and specific interaction with phosphatidylinositol 4-phosphate ( pi4p ) , which is a phosphatidylinositol lipid molecule found in most subcellular locations , predominantly at the golgi . ndrg1 also strongly interacts with pi4p and participates in vesicle formation , transportation , and sorting cargo proteins to endosomes . ndrg1 is a rab4a effector protein that is localized at perinuclear recycling / sorting vesicles in the trans - golgi network by binding to pi4p . therefore , it is possible that golph3 might regulate ndrg1 by competitively binding to pi4p , which promotes vesicles transport of the related receptors to the membrane . in summary , we demonstrated that knockdown of golph3 promoted glioma cell apoptosis and increased the cleavage of caspase 3 . in addition , we found negative correlation of golph3 and ndrg1 expression in glioma tissues and provided evidence that golph3 is involved in the downregulation of ndrg1 expression in glioma cells . further exploration the relationship of golph3 and ndrg1 may provide a potential candidate for the therapy of glioma and guide its prognosis .
backgroundgolgi phosphoprotein 3 ( golph3 ) has been reported to be involved in the development of several human cancers . 
 our previous study showed that golph3 expression in glioma tissues was related to the severity of the malignancy of the cancer . 
 however , the mechanism by which golph3 affects cell apoptosis is largely unknown . 
 the present study was designed to explore the possible mechanism of golph3 in cell apoptosis.material/methodsto analyze the biological role of golph3 in glioma cells , we used golph3 small interference rna in apoptosis of glioma cells . 
 the apoptosis of glioma cells was detected by flow cytometry . 
 the expression level of golph3 and ndrg1 protein was determined by western blot analyses and immunohistochemical staining , respectively , to evaluate their association with glioma . 
 tumor tissues were collected from patients with glioma . 
 normal cerebral tissues were acquired from cerebral trauma patients undergoing internal decompression surgery.resultswe confirm that the decrease of golph3 that promotes the apoptosis of glioma cells may be regulated by the activation of ndrg1 and cleaved capcase 3 . there was a inverse association between golph3 and ndrg1 in glioma samples.conclusionsour findings 
 indicate that golph3 and ndrg1 both play an important role in glioma etiology . 
 either golph3 or ndrg1 might be a potential candidate for malignant glioma therapy .