fasciolosis , caused by the parasites of fasciola spp . , is regarded as one of the important parasitic disease among the neglected tropical diseases . although not long time ago , it was considered typically as a veterinary issue , but at present ranks among the highest significant food borne diseases by who , fao and many researchers ( 1 ) . estimates of human infection in asia and africa vary from 2.4 - 17 million people ( 2 ) . it was estimated in 2005 that about 56.2 million people were infected with food - borne trematodes , 79 million had severe sequelae , and the global burden was 665,352 dalys , of which a most important share was attributed to fasciolosis ( 4 ) . in iran , until 1989 , human fascioliasis was sporadic in iran with several case reports of human infections but after occurring two important outbreaks of fasciolosis in 1989 and 1999 , during which the world largest ever outbreaks occurred in gilan province , northern iran and affected more than 10000 people in each of which ( 5 - 8 ) the feature was changed and its importance became obvious . following the occurrence of the various features of fasciolosis , the need for verifying the prevalence in different areas of iran was palpable . indeed lots of challenges on the different aspects of the disease , including treatment , diagnosis , following up , incidence , prevalence etc now have been arisen which caused the researcher of the country to focus more on this disease . the present study was based on the aforementioned need to verify the prevalence of fasciolosis in one of the most talented areas of iran in the context of having human fasciolosis , i.e. ardabil provinces , northwestern iran . we tried to detect the rate of prevalences and different epidemiological aspects of human fasciolosis in meshkin - shahr in that province because of its nearby locality to most critical provinces of fasciolosis and its ecological situation . it covers an area of approximately 1530 km and its population is estimated to be 237 585 , among whom 29.7% are settled in urban areas and 70.3% live in 323 rural areas ( https://en.wikipedia.org/wiki/meshginshahr ) . the distribution of the population in terms of urban and rural residency is equal ( fig 1 ) . overall , 458 serum samples were collected by randomized cluster sampling method from 153 males and 305 females referred to different health centers of the region after recalling by staff in those centers in 2012 . therefor the sampling was such that randomly a representative sample of the society was surveyed for the disease . sera were collected from 4 rural points of high , koojengh , youssef kahn , and naghdi olia and four regions of urban areas as gharehdarvish , sayyedabbad , farhangian and parasil . sera were stored in refrigerator at -20 c , and then were sent to dept . of medical parasitology , school of public health , tehran university of medical sciences , iran for examination with elisa . liver fluke homogenate ( lfh ) antigen was prepared by homogenizing adult worms in 0.045 m pbs / ph 7.2 using electrical homogenizator ( edmund buhler co. , model homo 4/a mituhr ) followed by sonication ( tommy seiko model up-200p , tokyo ) , and then centrifugation at 15000 g at 4 c for 30 min ( table 1 ) . ten g /ml antigens ( lfh ) , serum dilutions of 1:500 and conjugate anti - human coombs with 1:10000 dilutions were utilized to perform the test . the cut - off value was calculated as means plus 3.0 standard deviation od value of the healthy group sera . it covers an area of approximately 1530 km and its population is estimated to be 237 585 , among whom 29.7% are settled in urban areas and 70.3% live in 323 rural areas ( https://en.wikipedia.org/wiki/meshginshahr ) . the distribution of the population in terms of urban and rural residency is equal ( fig 1 ) . overall , 458 serum samples were collected by randomized cluster sampling method from 153 males and 305 females referred to different health centers of the region after recalling by staff in those centers in 2012 . therefor the sampling was such that randomly a representative sample of the society was surveyed for the disease . sera were collected from 4 rural points of high , koojengh , youssef kahn , and naghdi olia and four regions of urban areas as gharehdarvish , sayyedabbad , farhangian and parasil . sera were stored in refrigerator at -20 c , and then were sent to dept . of medical parasitology , school of public health , tehran university of medical sciences , iran for examination with elisa . liver fluke homogenate ( lfh ) antigen was prepared by homogenizing adult worms in 0.045 m pbs / ph 7.2 using electrical homogenizator ( edmund buhler co. , model homo 4/a mituhr ) followed by sonication ( tommy seiko model up-200p , tokyo ) , and then centrifugation at 15000 g at 4 c for 30 min ( table 1 ) . ten g /ml antigens ( lfh ) , serum dilutions of 1:500 and conjugate anti - human coombs with 1:10000 dilutions were utilized to perform the test . the cut - off value was calculated as means plus 3.0 standard deviation od value of the healthy group sera . there was no significant difference as regards age groups , sex , job , residency , literacy and consuming row vegetable . age wise prevalence showed 3.3% as the highest rate in age group of 40 - 49 yr old ( table 2 ) . according to job , unemployment subjects had the highest rate of infection as 5.88% , followed by 4.08% in students , 3.6% in farmers and 1.18% in housewives . the seroprevalence of infection was 1.52% in illiterate people , 9% in subjects with bsc , 2.6% with diploma , 2% with less than diploma and 0% with doctoral documents but the difference was not significant . as for residency , urban life showed no significant difference with rural life ( 2.4% vs 1.42% ) . in this study we could detect the seroprevalence of human fasciolosis as 1.96% in meshkinshahr , ardabil province . this study follows the continuous studies conducted in iran to determine the situation of human fasciolosis . this province has required criteria as regards involving with parasitic diseases ( 10 - 12 ) . among these criteria ecological situation , adjacent to endemic areas of fasciolosis , culture , food habitation , etc might be named . the rate of 1.96% seropositivity shows that further monitoring is needed to detect the real situation of the disease in the area . similar to other serological studies it is not possible to judge the real rate of infection and further diagnostic methods such as stool exam are necessary . although in iran , two outbreaks of human fasciolosis during the years of 1989 and 1999 have been occurred called the world s largest ever outbreaks ( 5,6 ) , forghan - parast and ashrafi ( 7 ) but recent surveys reveal prevalence of 0.4% and 1.2% using coprological and serological methods , respectively , confirming a hypoendemic situation ( 13 ) . world health organization ( who ) included iran among six countries which are known to have a serious fasciolosis problem ( 14 ) . table 2 shows the rate of human fasciolosis through different studies conducted in iran . in northern endemic areas of iran , numerous irrigation canals , agricultural crop traditions ( mainly rice ) , temperatures higher than 20 c , high rainfall ( 1300 - 1800 mm annually ) , and short dry seasons , favor fasciolosis transmission and lymnaeid populations ( 15 ) . fuentes et al . have demonstrated by mathematical modeling the impact of annual rainfall in determining the level of transmission in endemic areas ( 16 ) . all these data are witness to necessity of monitoring fasciolosis in iran . in our study the highest rate of infection was seen in age group of 40 - 49 yr old as 3.3% . in the outbreaks in gilan and kermanshah the highest number of infected individuals was seen in the 10 - 29 year and in 10 - 19 year age groups , respectively . in comparison , in non - epidemic situations , the numbers of infected cases were higher in older ( > 20 year ) age groups ( 13,17 ) . generally , the pattern of age wise prevalence shows that in iran , adults are infected more than children ( 10,15 ) . in comparison to other countries of the world especially those of nearby region , the population at risk of human fasciolosis in egypt is considered to be 27 million ( 18 ) and the prevalence ranging from 2 - 11% ( 19 ) . the 0.31% prevalence of human fasciolosis has been reported in lahor , based on fecal exams performed in 2003 - 2005 ( 21 ) . in yemen human fasciolosis was indentified in 0.5% of 37,000 people during the period 1980 - 1982 ; fasciola ova were found 185 cases ( 22 ) . it shows that fasciolosis is a disease of immediate topic for consideration not only in iran but in all other countries . obtained seroprevalence of fascioliasis in meshkin - sshahr shows that a global study to detect the rate of fasciolosis is necessary in iran . health authorities should pay attention to prevent of spreading the disease through public education , mass treatment , ecological consideration , etc . the ease of sending local foods from endemic areas and frequency of intermediate hosts has prepared a situation which increases the possibility of involving with the disease .
backgroundthe aim of this study was to conduct a seroprevalence survey in meshkin - shahr , ardabil province , north western iran to detect the rate of human fascioliasis in the city and nearby villages . 
 literature shows that no such study has been conducted so far.methodsoverall , 458 serum samples were collected by randomized cluster sampling method from 153 males and 305 females referred to different health centers of the region after recalling by staff in those centers in 2012 . 
 all cases filled out a questionnaire and an informed consent . 
 sera were analyzed using indirect - elisa test . 
 ten g /ml antigens ( liver fluke homogenate ) , serum dilutions of 1:500 and conjugate anti - human coombs with 1:10000 dilutions were utilized to perform the test . 
 data analysis was conducted using spss software ver . 
 18.resultsnine cases ( 1.96% ) were positive for fascioliasis by elisa test . 
 the seroprevalence of fascioliasis among females was 1.63% and 2.6% in males . 
 there was no significant difference as regards age groups , sex , job , residency , literacy and consuming row vegetable . according to job , 
 unemployment subjects had the highest rate of infection as 5.9% . 
 the seroprevalence of infection was 1.52% in illiterate people . 
 as for residency , urban life showed no significant difference with rural life ( 2.4% vs 1.42 ) . 
 age group of 40 - 49 yr old , with 3.3% seropositivity had the highest rate.conclusionobtained seroprevalence of fascioliasis shows immediate attention of health authorities to the diseases in the area . 
 the adjacent of ardabil province to endemic areas of fasciolosis accentuates this attention .