the indigenous microbiota plays an important role in health and diseases of the humans and animals . it contributes to the development of the immune system and provides resistance to colonization by allochthonous or pathogenic microorganisms . oral diseases seem to appear after an imbalance among the indigenous microbiota , leading to the emergence of potentially pathogenic bacteria . immunoglobulins are proteins of the animal origin endowed with known antibody activity and for certain other proteins related to them by chemical structure . five distinct classes of immunoglobulin molecules are recognized in higher mammals , namely igg , iga , igm , igd , and ige . secretory iga ( siga ) constitutes the predominant immunoglobulin isotype in secretions , including saliva . it is considered to be the first line of defense of the host against pathogens , which colonize or invade surfaces bathed by external secretions . secretory iga can traverse mucosal membranes ; in this way , it helps to prevent the entry of infectious microorganisms . an increase in the secretion rate is accompanied by a decrease in concentration of siga in saliva . smoking has been shown to affect t - cell subsets , natural killer cells , and serum immunoglobulin concentrations . decrease of s - iga may be due to an influence on the salivary gland cells responsible for the completion of the s - iga or on the cells of immunologic system involved in the production of the iga molecules . many studies were performed relating the physiology of iga secretion occurrence of recurrent aphthous stomatitis ( ras ) . ras affects approximately 20% of general population , but when specific ethnic or socioeconomic groups are studied , the incidence ranges from 5% to 50 % . increased circulating levels of antibody against oral mucous membrane may be found in affected individuals . these increased antibody levels may be due to an immunologic cross - reaction between oral epithelium and indigenous microorganisms or the exposure , by ulceration , of previously sequestrated , hidden antigens setting up cycles of recurrent disease . this study was carried out to find if patients with recurrent aphthous ulcers show any abnormality in the immunoglobulin levels and also evaluation of immunoglobulin levels in tobacco smokers . the study and control population of approximately 80 subjects were considered for this study which were drawn from the outpatient department . twenty subjects with a provisional diagnosis of ras was made on the basis of clinical examination described by stanley and 40 subjects with chronic smoking habits were included in the study group . age and sex matched apparently healthy 20 subjects who had no recent history of systemic conditions were included in the control group . the patient 's personal history was recorded with tobacco smoking habits , frequency , and duration of smoking . in each case approximately 1.0 ml of unstimulated saliva was collected from each subject by drawing the saliva with a disposable syringe and later passing it into a sterile glass centrifuge tube . the samples were immediately frozen at 20 c , and maintained at that temperature until shortly before assay . assay samples were thawed at 37 c , and then immediately centrifuged at 12,000 rpm for 10 min and the supernatant fluid was used for assay of iga using quantia - iga turbidimetric immunoassay . the iga levels are expressed as mean  sd and range values with coefficient of variation ( cv % ) . since normality was not presumed in siga levels , a nonparametric method , the mann for all the tests , a p - value of < 0.05 was considered statistically significant . the iga levels are expressed as mean  sd and range values with coefficient of variation ( cv % ) . since normality was not presumed in siga levels , a nonparametric method , the mann for all the tests , a p - value of < 0.05 was considered statistically significant . this study comprised of 80 subjects which include 20 controls in group i , 40 smokers in group ii , and 20 cases of recurrent aphthous ulcers in group iii . table 1 shows age and sex distribution in groups i , ii , and iii . age and sex distribution in groups i , ii , and iii the value of siga in controls ranged from 0.07 to 0.31 comparison of siga levels between the control group and study group the siga levels in patients with recurrent aphthous ulcers ranged from 0.03 to 1.02 siga levels in the control and study group among the 40 patients with chronic smoking habits , 19 individuals had the habit of smoking 19 cigarettes per day and had a mean siga level of 0.11 g / l , 12 individuals had the habit of smoking 1019 cigarettes per day and had a mean siga level of 0.20 g / l , and 9 individuals had the habit of smoking 20 or more cigarettes per day and had a mean siga level of 0.09 siga levels in relation to smoking frequency the anova test for the smoking frequency and siga levels was of a p - value of 0.23 . however , correlation between siga levels and smoking frequency was not statistically significant . among the 40 patients with chronic smoking habits , 12 individuals had 510 years , 13 individuals with 1115 years , and 15 individuals with 1620 years of duration of smoking had mean siga levels of 0.14 siga levels in relation to duration of smoking the mean siga level in groups i and ii was 0.07 g / l , and there was a significant difference of ( p < 0.01 ) between groups i and ii . however , there was no significant difference between groups i and iii , and ii and iii [ table 3 ] . comparison of siga levels between the control group and study group the siga levels in patients with recurrent aphthous ulcers ranged from 0.03 to 1.02 among the 40 patients with chronic smoking habits , 19 individuals had the habit of smoking 19 cigarettes per day and had a mean siga level of 0.11 g / l , 12 individuals had the habit of smoking 1019 cigarettes per day and had a mean siga level of 0.20 g / l , and 9 individuals had the habit of smoking 20 or more cigarettes per day and had a mean siga level of 0.09 siga levels in relation to smoking frequency the anova test for the smoking frequency and siga levels was of a p - value of 0.23 among the 40 patients with chronic smoking habits , 12 individuals had 510 years , 13 individuals with 1115 years , and 15 individuals with 1620 years of duration of smoking had mean siga levels of 0.14 g / l , and there was a significant difference of ( p < 0.01 ) between groups i and ii . however , there was no significant difference between groups i and iii , and ii and iii [ table 3 ] . salivary immunoglobulin a ( siga ) is produced by local plasma cells situated in the mucosa and salivary glands and transport protein by ductal epithelial cells . two or three iga molecules are united with transport protein to form the composite molecule which is secreted as salivary iga . tobacco use is the single biggest contributor to ill health , and is the most important preventable cause of death . the ill effects of tobacco include cardiovascular disorders , respiratory disorders , and lung cancer . in addition , the risk of oral cancer and potentially malignant lesions is higher among smokers compared with those who have ever smoked . much work has been conducted on systemic immune status but , surprisingly , the influence of smoking on mucosal immunity has been relatively neglected . smoking impairs t - cell immunoregulation of b - cell differentiation and maturation thus leading to a decrease in siga levels . smokers have increased polymorphonuclear neutrophil counts , decreased natural killer cell activity , an increased total t - cell numbers with a decrease in the t helper / suppressor cell ratio in heavy smokers leading to decreased immunoglobulin a levels . low immunoglobulin levels are important predisposing factor in the development of infections associated with smoking , such as chronic bronchitis . however , some authors say that salivary iga has no definitive role in the pathogenesis of aphthous ulceration . these values were higher compared to that of lehner ( 13 mg/100 ml ) , olson et al . ( 6.2  0.4 mg/100 ml ) , baron et al . in edinburg subjects ( 92145 g / ml ) and in cairo workers ( 120200 g / ml ) , norhagen engstrrom ( 151.8 mg / l ) , and griesel and germishuys ( 11.0  3.1 mg / dl ) . the exact reason for the variations in the levels in controls is not clear , and it can be due to variation in the methodology and sampling . these values were close to the values established by bennet and reade ( 0.050.18 g / l ) . ( 6.7  0.3 mg/100 ml ) , barton et al . in edinburg subjects ( 4144 g / ml ) , in cairo workers ( 80180 g / ml ) , and griesel and germishuys ( 10.4  2.9 mg / dl ) . variation in the level of immunoglobulin may be due to smoking , which has several toxic effects , so it is possible that the immune system is also effected in some way , leading to decreased antibody production . smoking also impairs t - cell immunoregulation of b - cell differentiation and maturation leading to decreased immunoglobulin a levels . g / l . these values were higher compared to that of lehner ( 15 mg/100 ml ) , ben - aryeh et al . in the dormant phase 316 mg/100 ml and in the acute phase 1.515 mg /100 ml , g / l , and there was a significant difference ( p < 0.01 ) between groups i and ii . these findings are similar to bennet and reade hersey et al . , and barton et al . who demonstrated decreased immunoglobulin a levels . smoking impairs t - cell immunoregulation of b - cell differentiation and maturation leading to decreased siga levels . the mean siga levels in groups i and iii was 0.11 these findings are similar to lehner , ben - aryeh et al . , and bennet and reade . although being non - specific , estimation of siga levels can contribute to diagnosis of ras and can be used as a diagnostic marker in ras and can also be used to analyse the influence of smoking on immunoglobulins . however , the findings of this study needs to be carefully interpreted because of the small sample size and to the best of our knowledge , lack of studies involving the estimation of siga levels in patients with ras and smokers . further research involving larger samples and estimation of siga levels during the course of disease and treatment is suggested along with extensive work involving specificity of estimation techniques before a definite statement on decreased siga levels and their clinical applications can be made .
objectives : the aim of the present study was to analyze the influence of smoking on the salivary immunoglobulin response in smokers and to evaluate the salivary immunoglobulin a in patients with recurrent aphthous ulcers.materials and methods : the study included total of 80 subjects , of whom 40 were having history of chronic smoking habit , 20 were clinically diagnosed cases of recurrent aphthous ulcer and 20 were in the control group . 
 sample of unstimulated saliva was collected , centrifuged and analyzed for the level of salivary immunoglobulin a with turbidimetric immunoassay . 
 for all the tests , a p- value of < 0.05 was considered for statistical significance.results:the mean salivary immunoglobulin a level in control group was 0.20 grams / litre and in smokers the mean salivary immunoglobulin a level was 0.13 grams / litre . in patients with recurrent aphthous ulcers 
 mean salivary immunoglobulin a level was 0.31 grams / litre . 
 the mean salivary immunoglobulin a levels showed a decreasing trend from controls to smokers . 
 these results were highly significant for values between control groups to smokers.conclusion:the mean salivary immunoglobulin a levels demonstrated a progressive decrease from controls to smokers . 
 this investigative procedure although non - specific , can be used as a diagnostic marker in smokers and patients with recurrent aphthous ulcers .