the development of sulfonamides is a fascinating and informative area in medicinal chemistry [ 13 ] . its functional group has a long and rich history in organic chemistry and drug discovery [ 4 , 5 ] . the p - toluenesulfonamide and benzenesulfonamides have been widely explored in synthetic chemistry [ 4 , 5 ] ; however , few work has been done on the -tolylsulfonamide . for instance , synthesis of benzenesulfonamide derivative of pipecolic acid   and that of glycine   had been reported . in addition , benzenesulfonamide of alanine was achieved as a result of synthetic usage of such amino acid as a linker to 6h-1,3,4-thiadiazine scaffold   while naphthylsulfonamide was prepared as antagonist of chemokine receptor . domagk 's discovery of antibacterial activity for the azo dye prontosil led to the first effective chemotherapeutic agent , sulfanilamide . a retrospective look at sulfonamides leaves no doubt that besides providing the first effective treatment of bacterial infections [ 10 , 11 ] , they also unleashed an antibiotic revolution in medicine [ 1215 ] to rationally design new therapeutic agents [ 16 , 17 ] . these compounds provided an excellent lead for structural modification and ushered in the modern era of chemotherapy and drug design . sulfonamides inhibit the multiplication of bacteria by acting as competitive inhibitors of p - aminobenzoic acid ( paba ) in the folic acid metabolism cycle [ 18 , 19 ] . in fact , the discovery that sulfonamides act through folate inhibition resulted in the development of dihydrofolate reductase inhibitors such as trimethoprim [ 20 , 21 ] . furthermore , sulfonamide moiety has a crucial functionality because of its wide variety of reported biological [ 2225 ] and pharmacological activities such as anticancer [ 2628 ] , carbonic anhydrase inhibitory [ 2931 ] , antibacterial [ 3234 ] , antimalarial [ 35 , 36 ] , antitumor [ 37 , 38 ] , antihypertensive , anti - inflammatory [ 4042 ] , and antiprotozoal activities . some sulfonamides have been established as potent drugs in treatment of insomnia and other sleepless challenges in man by antagonizing orexin neural activity [ 4446 ] . sulfonamide has also been reported to possess good herbicidal   and corrosion inhibitory properties [ 49 , 50 ] . for instance , methicillin is a good antibacterial agent , yet , methicillin resistance among staphylococcus aureus   and staphylococcus epidermidis [ 53 , 54 ] as well as other drug resistance in escherichia coli   had been identified to be of great concern in public health . epidemiological studies have also revealed that the emergent of new diseases is on the increase and quite alarming [ 56 , 57 ] . in addition , the choice of s. aureus and e. coli as the targeted organisms in this study was due to wide range of infectious diseases and life threatening conditions associated with such isolates . s. aureus , which produces heat stable toxin , is among the invasive gram positive known as pyogenic cocci implicated in several diseases of human [ 58 , 59 ] . from the literatures , infections caused by methicillin - resistant s. aureus ( mrsa ) and vancomycin - resistant s. aureus are associated with high morbidity and mortality , high treatment cost , and long stays in hospitals . e. coli , a facultative anaerobe of wide distribution in the environment , has been implicated in the cause of urinary tract infections , meningitis , sepsis , wound infections , nosocomial pneumonia , and arthritis . a subgroup enterohemorrhagic e. coli ( ehec ) can cause severe potentially fatal illness known as hemorrhagic colitis with symptoms of blood diarrhea and severe abdominal pain . based on the numerous applications of sulfonamides and challenges associated with drug usage and multidrug resistance microorganisms aforementioned among others , there is a continuous need for the synthesis of new organic compounds as potential antimicrobial agents for the replacement of the old existing ones currently available in the market or to enhance the potency of the former ones . thus , it is conceivable to develop a series of functionalized sulfonamides in order to investigate the in vitro antibacterial activities of such scaffolds on the targeted organisms , namely , s. aureus and e. coli . in the continuation of our effort on the discovery of rationally designed therapeutic agents [ 62 , 63 ] , we have herein reported the synthesis of a series of new alkylarylsulfonamide with potent antibacterial activity . we started with the reaction of equimolar proportion of readily available amino acid , l - pipecolic acid with -toluene sulfonylchloride ( -tscl ) via a continuous magnetic stirring at room temperature in the presence of aqueous sodium carbonate for 48  h according to a known procedure . after the completion of the reaction which was tlc monitored , the excess unreacted -tscl was recovered with dichloromethane ( dcm ) using separatory funnel while the aqueous layer was worked up by acidifying to a ph of 2.2 to get clear solution which upon freeze drying and column purification afforded 1-(benzylsulfonyl)piperidine-2-carboxylic acid 1 in excellent yield ( 98% ) . being motivated by this encouraging discovery , we then proceeded on the coupling of nine other amino acids with -tscl under similar condition to obtain -tolylsulfonamides 210 in good to excellent yields ( scheme 1 ) . in contrast to monosulfonylation that occurred in formation of 110 , the synthesis of 11 was achieved in higher yield only when disulfonylation of the required amino acid ( tyrosine ) was utilized . this was noticed when monosulfonylation of tyrosine afforded 11 in lower yield ( 45% ) when compared with other sulfonamide products 110 with their yields ranging from 87.5% to 98.8% . this was because under the monosulfonylation of tyrosine , the reaction terminated when the -tscl , being the limiting reagent , has been exhausted living excess of the tyrosine in the aqueous medium . this was as a result of two nucleophilic attacking sites available on the l - tyrosine moiety . this means that the phenolate anion was competing with the amino group in terms of the nucleophilic potential on the -tscl precursor , thereby resulting in an unusually lower yield ( 45% ) . based on this development , the stoichiometric ratio was revisited in the production of 11 to give allowance for selective disulfonylation of tyrosine . hence , the reaction for the production of 11 was reconducted in such a way that the molar equivalent of -tscl was doubled that of tyrosine in order to accommodate nucleophilicity from both nh2 and phoof the latter , then the yield for 11 increased drastically to 89.6% ( scheme 2 ) . structures of all eleven -tolylsulfonamides were confirmed by spectroscopic means which include ir , mass spectra , h , and c nmr as well as the elemental analytical data . thus , the detail structural elucidation was here - in confirmed using compound 1 as the representative of the -tolylsulfonamide templates . the infrared spectrum of compound 1 gave rise to the absorption bands of oh and carbonyl of carboxylic acid at 3422 and 1736  cm , respectively . also , the ch stretching of both aliphatic and aromatic occurred at the vibrational frequency of 2974 and 2822  cm , respectively . the two ir bands for so2 unit were observed at 1159 and 1238  cm as expected , whereas the band accountable for c = c of aromatic was observed at 1603  cm . the only easily accountable band resulting from a bending vibrational mode at the finger print region was ar - h which appeared at 700  cm . in the h - nmr of compound 1 , all the five aromatic protons resonated downfield as a singlet at  7.52  ppm while the ch2 of benzylic group appeared as a two - proton singlet at  4.28  ppm . considering the upfield region , it was discovered that ch proton adjacent to cooh resonated as a doublet of doublet at  4.084.02  ppm with coupling constants of 3.44  hz and 15.12  hz . this was as a result of the fact that its ch2 neighbouring protons were not totally chemically equivalent even though they were attached to the same carbon atom . all other remaining pipecolic protons were well accounted for upfield between  3.573.53 and 1.811.68  ppm . the justification for their appearance near tms scale lies in the fact that they were attached to sp hybridized centre . although , the molecular ion peak was not observed , the base peak , however , occurred at m / z 178.1 which was as a result of the loss of benzylic radical . other prominent peaks which were formed as a result of some fragmentation patterns appeared at m / z 180.1 , 179.1 , 165.1 , 121.0 , 77.0 , and 64.0 with the intensities of 55% , 65% , 30% , 42% , 13% , and 31.7% , respectively . the second stage of this experiment involved the chemical transformation of the carboxylic acid side chain moieties of the sulfonamides 111 into n , n - diethyl alkanamides 1222 using highly efficient nonconventional method in its slightly modified version . the reaction optimization for this aspect was carried out using amination of 1 with diethyl amine under triethyl amine basified condition as a representative . thus , having established the c - amidoalkylation of the sulfonamide 1 to achieve the alkanamide 12 in higher yield as a bountiful success , we were motivated to extend our investigation to the reaction of other -tolylsulfonamides 211 with diethylamine under the same condition ( scheme 3 ) . these attempts also afforded the n , n - diethyl - substituted products 1322 in good to excellent yields as envisaged . the spectroscopic assignment was consistent with that of proposed structures for the obtained n , n - diethyl alkanamides 1222 . so , it is necessary to consider the spectral data of 12 as a typical representative of the n , n - diethyl alkanamides in order to authenticate such compounds . the ir spectrum of 12 had no absorption band above 3028  cm indicating the absence of oh bond . the bands at 3028 and 2951  cm depicted ch of aromatic and aliphatic , respectively . also , the c = o and c = c frequency appeared at 1720 and 1593  cm , respectively , while the two bands of so2 were observed at 1188 and 1148  cm . furthermore , the chemical shifts and multiplicity patterns of h- and c - nmr correlated well with that of the proposed alkylated sulfonamides 1222 . the h - nmr spectrum of 12 in d2o showed five aromatic protons as singlet at  7.54 and ch2 of benzylic group as singlet at  4.30 . one triplet at  1.401.37 ( 6h ) was assigned to two methyl of diethyl group that were chemically equivalent while one quartet at  3.213.16 ( 4h ) was assigned to two methylene protons adjacent to the methyl protons with a coupling constant of 7.20  hz . the c - nmr of 12 in d2o in the presence of a drop of dioxane revealed the presence of seventeen different carbon atoms with c = o having the highest signal at  173.5  ppm while the ch3 carbon atoms appeared to have the least signals at  11.4  ppm .   the general antibacterial sensitivity testing ( inhibition zone , mm ) of all the series of twenty - two synthesized sulfonamides alongside with that of streptomycin clinical standard was assayed on test organisms ( e. coli and s. aureus ) using agar diffusion technique . the choice of e. coli as the gram ve organism is because it is easily transmissible through food , water , soil , animal , and man . e. coli is a normal flora of human body which causes a lot of vancomycin - resistant enterococci and methicillin - resistant staphylococcus aureus ( mrsa ) . based on our previous report , the choice of streptomycin as clinical standards is due to the fact that , at low concentrations , streptomycin only inhibits the growth of the bacteria through induction of prokaryotic ribosomes to misread mrna   and it also possesses broad spectrum of antibacterial activity . there were reported cases of e. coli and staphylococcus aureus being susceptible to streptomycin [ 4 , 68 ] . the biological relevance of the synthesized sulfonamides here - in was authenticated by screening them in vitro against staphylococcus aureus atcc 6538 ( s. aureus ) and escherichia coli atcc 25922 ( e. coli ) with the reported selectivity index ( si ) duly calculated from zones of inhibition ( zoi ) . hence , it should be noted afterwards that the abbreviated forms were given in the bracket for the sake of brevity and conciseness .   from the result of sensitivity testing , probable activities of -tolylsulfonamide family on the test organisms were categorized based on the size of zone of inhibition ( table 1 ) . interestingly , it was observed that some of the compounds exhibited probable significant activities based on the large zone of inhibition reported . for instance , compounds 6 , 7 , 12 , 14 , and 22 were highly active on e. coli while compounds 1 , 2 , 4 , 5 , 10 , 11 , 17 , and 20 exhibited moderate activities on the same organism . all other compounds showed low activities on e. coli except 3 and 16 which showed no activity at all on the e. coli even at 1000  g / ml . the scenario of comparative study of effect of the sulfonamides and streptomycin on e. coli could be vividly understood by observing the selectivity index ( si ) . all the sulfonamides have selectivity indices ranging from 0.29 for compound 18 to 0.96 for compound 22 ( i.e. , less than 1 ) . this implies that streptomycin ( si = 1 ) was probably more active than any of the sulfonamide scaffolds as regarding the inhibition of e. coli growth . in the same vein , looking through the effect on s. aureus , compounds 2 , 5 , 17 , 21 , and 22 were highly active ; 4 , 6 , 8 , 10 , 11 , 12 , 14 , and 19 were moderately active ; 1 , 3 , 7 , 9 , 13 , 15 , 18 , and 20 exhibited low activity while 16 showed no activity on s. aureus ( table 1 ) . the comparative study of -toluenesulfonamides to streptomycin on s. aureus growth inhibition is worthy , of commendation . from the si values , compounds 9 , 12 , 18 , and 20 competed favourably with streptomycin while 2 , 4 , 5 , 6 , 8 , 13 , 14 , 17 , 19 , 21 , and 22 ( si = 1.082.31 ) showed even a better activity than streptomycin on s. aureus . all other compounds exhibited lesser activity than streptomycin ( si = 0.460.92 ) on s. aureus except 16 which showed no activity .   due to high zones of inhibition obtained during general sensitivity testing , the minimum inhibitory concentration ( mic ) was conducted , first at 100  g / ml using russell and furr method . however , those compounds that could not affect the inhibition of microbial growth at this concentration were further repeated for mic test at 1000  g / ml . the result of the mic of this class of compounds on e. coli and s. aureus was as shown in table 2 . interestingly , all the sulfonamides tested showed a concentration - dependent inhibitory effect on the in vitro microbial growth assays . considering the mic testing on the gram negative organism ( e. coli ) , it was observed that compounds 1 , 6 , 7 , 11 , 12 , 14 , and 22 inhibited the microbial growth at varying values less than or equal to 100  g / ml , whereas , all other compounds were active on e. coli at higher concentration ( between 125 and 1000  g / ml ) except 3 and 16 which had no activity even at 1000  g / ml . specifically , mic values of the synthesized compounds on e. coli were reported to be 50  g / ml for 12 and 14 ; 100  g / ml for 1 and 11 ; 125  g / ml for 2 , 4 , 5 , 10 , 17 , and 20 ; 250  g / ml for 8 , 9 , 13 , 15 , 19 , and 21 . although , the most active sulfonamides on e. coli were 6 , 7 , and 22 with mic values of 25 , 25 , and 12.5  g / ml , respectively , none of them could compete with streptomycin ( mic value of 6.25  g / ml ) in terms of activity . the two rings presence in 22 and their  character might be responsible for it being the most active as deduced from the finding of aissaoui and coworkers . in addition , eight sulfonamides ( 2 , 5 , 6 , 8 , 14 , 17 , 21 , and 22 ) inhibited the s. aureus growth at concentration ranging from 1.8 to 100  g / ml . all other compounds were able to affect the expected inhibition from 125  g / ml to 1000  g / ml except 16 which had no activity even at 1000  g / ml . the significant antibacterial activity of the synthesized compounds may be explained by the ability of its sulfonamide binding site to mimic p - aminobenzoic acid ( paba ) which is an essential growth factor in the targeted organisms as earlier documented in the literatures [ 1416 ] . the explanation for this encouraging activity of the synthesized sulfonamides could be traceable to the mode of action of sulfonamide drugs . this is based on the inhibition of dna synthesis   by interfering with para - aminobenzoic acid ( paba ) in biosynthesis of folic acid . furthermore , from the structure activity relationship ( sar ) study , it was observed that the nature of side chains ( r1 and r2-ch - r3 ) of the -tolylsulfonamides and the presence of n , n - diethylated amido moieties { ( ch3ch2)2n  c = o } of the amide contributed immensely toward synergistic or antagonistic effect on the reported in vitro antibacterial activity . for instance , the antibacterial activity of structurally related -tolylsulfonamides containing r1 = ( ch2)2sch3 , 6 ; r2 = r3 = ch3 , 7 , on e. coli were very high with mic value of 25  ppm whereas the activity reduced to moderate when r1 = ch3 , 4 ; r1 = ch2sh , 5 with mic value of 125  ppm . the synergistic effect due to the presence of n , n - diethylamido moieties was noticed in some synthetic conversions such as 1  12 , 3  14 and 11  22 . thus , -tolylsulfonamides 1 and 11 had moderate activity on e. coli while their corresponding diethylamido 12 and 22 had better and higher activity on e. coli . in fact , -tolylsulfonamide 3 had no activity on e. coli whereas its n , n - diethyl amidated counterpart , 14 had very high activity with mic value of 50  ppm . the synergistic effect noticed herein might be as a result of electron donating nature of the r1 side chain . on the contrary , antagonistic effect was observed in the synthetic modification of 5  16 and 6  17 as far as presence of n , n - diethyl - substituted amido moieties was concerned . this reverse order of activity on e. coli might be as a result of the presence of sulfur atom in 5 and 6 which might have altered the electron donating prowess . considering the structural relationship effect on the activity of the synthesized compounds against s. aureus , it was discovered that n , n - diethyl - substituted amides 14 , 17 , 21 , and 22 had better activity than their -tolylsulfonamide precursors 3 , 6 , 10 , and 11 . it was discovered that aqueous medium approach at ambient temperature used here - in was a highly efficient procedure for the preparation of various -tolylsulfonamide derivatives 111 in good to excellent yield . the synthetic modification of these derivatives as precursors to furnish disubstituted amide bearing sulfonamides 1222 was also successfully achieved . the antibacterial evaluation study showed 1-(benzylsulfonyl ) pyrrolidine-2-carboxylic acid , 2 to be the most active sulfonamide on s. aureus while 4-(3-(diethylamino)-3-oxo-2-(phenylmethylsulfonamido)propyl)phenylphenylmethane sulfonate , 22 emerged as the most active on the growth inhibition of e. coli . thus , this work will be very useful for further studies in terms of toxicity effect and structural activity relationship ( sar ) study by using various types of dialkylated substituents to monitor the trend of improvement on their biological and pharmacological properties . the h - nmr spectra were recorded in d2o on nmr bruker dpx 400 spectrometer operating at 400  mhz . tms was used as an internal standard with the deuterium signal of the solvent as the lock and chemical shifts  recorded in ppm . the c - nmr spectra were run in addition of a few drops of dioxane but at 100  mhz frequency . the melting points were determined on xt-4 digital binocular microscope melting point apparatus manufactured by beijing technical instrument co. ltd . and were uncorrected . ir spectra were run on varian excalibur he 3100 ft - ir spectrometer while the mass spectra were obtained using waters gct premier spectrometer . the elemental analyses ( c , h , and n ) of the compounds were performed using flash ea 1112 elemental analyzer . lyophilization was carried out where necessary by using fd-1 freeze drier while concentration and removal of solvents were achieved with re-2000b rotary evaporator . in addition , the ph was monitored and confirmed during acidification by using portable ph meter model phb4 . the reaction progress was monitored with tlc using chcl3/ch3oh solvent system and the developed plates were visualized under uv lamp and/or in iodine tank where necessary . column chromatographic purifications were carried out on merck silica gel f ( mesh 200300 ) . organic solutions were dried over anhydrous na2so4 and concentrated with a buchi rotary evaporator at reduced pressure . at all stages of the experiments , the synthetic protocols were effected in bone dried solvents under nitrogen atmosphere in dried glassware which were wiped with stream flow of nitrogen gas prior to use and socl2 was freshly distilled prior to use . other reagents were used directly after ascertaining the purity condition . to a solution of l - amino acid ( 5  mmol ) in h2o ( 6  ml ) was added na2co3 ( 1.113  g , 10.5  mmol ) with a continuous stirring until all the solutes had dissolved . the clear solution was cooled to 10c and -toluenesulfonyl chloride , -tscl ( 1.144  g , 6  mmol ) was added in three batches over a period of 1  h. it was warmed up to 0c and stirred there for 1  h. finally , the reacting mixture was then warmed up to room temperature and allowed to stir there for 48  h. the reaction was quenched by the addition of dcm ( 10  ml ) and transferred into separatory funnel where the excess of -tscl was removed by extraction . the aqueous layer was then worked up to give a clear solution by the addition of 2n hcl until the ph 2.2 was attained . the clear liquid was then lyophilized at 52c under reduced pressure for 12  h to obtain the crude solid product which was purified by column chromatography ( chcl3/ch3oh , 3  :  1 ) to afford -toluene sulfonamides 111 in excellent yields . ( 1 )  1-(benzylsulfonyl)piperidine-2-carboxylic acid , 1reagents : l - pipecolic acid , yield 98% , mp 248c ( dec ) , rf = 0.87 . h - nmr ( d2o , 400  mhz )  : 7.52 ( s , 5h , ar - h ) , 4.28 ( s , 2h , ch2-so2 ) , 4.084.02 ( dd , j1 = 3.44  hz , j2 = 15.12  hz , 1h , hooc - ch - ch2 ) , 3.573.53 ( m , 1h , cha of ch2-n ) , 3.163.10 ( m , 1h , chb of ch2-n ) , 2.402.36 ( m , 1h , ch ) , 2.011.92 ( m , 2h , 2  ch ) , 1.811.68 ( m , 3h , ch & ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.5 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 57.7 , 57.6 , 44.8 , 26.5 , 22.1 ( 2ch2 ) ppm . ir ( kbr ) cm : 3422 ( oh of acid ) , 2974 ( ch aromatic ) , 2822 ( ch 1603 ( c = c ) , 1238 , 1159 ( so2 two bands ) , 700 ( ar - h ) . % ] : 269.1 [ m - ch2 , 3.2% ] , 180.1 [ 55% ] , 179.1 [ 65% ] , 178.1 [ m - phch2 , 100% ] , 165.1 [ 30% ] , 121.0 [ 42% ] , 77.0 [ ph , 13% ] , 64.0 [ so2 , 31.7% ] . anal . calcd . for 283.35 c13h17no4s : c , 55.11 ; h , 6.05 ; n , 4.94 . found : c , 55.29 ; h , 5.94 ; n , 4.86 . reagents : l - pipecolic acid , yield 98% , mp 248c ( dec ) , rf = 0.87 . h - nmr ( d2o , 400  mhz )  : 7.52 ( s , 5h , ar - h ) , 4.28 ( s , 2h , ch2-so2 ) , 4.084.02 ( dd , j1 = 3.44  hz , j2 = 15.12  hz , 1h , hooc - ch - ch2 ) , 3.573.53 ( m , 1h , cha of ch2-n ) , 3.163.10 ( m , 1h , chb of ch2-n ) , 2.402.36 ( m , 1h , ch ) , 2.011.92 ( m , 2h , 2  ch ) , 1.811.68 ( m , 3h , ch & ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.5 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 57.7 , 57.6 , 44.8 , 26.5 , 22.1 ( 2ch2 ) ppm . ir ( kbr ) cm : 3422 ( oh of acid ) , 2974 ( ch aromatic ) , 2822 ( ch aliphatic ) , 1736 ( c = o of cooh ) , 1603 ( c = c ) , 1238 , 1159 ( so2 two bands ) , 700 ( ar - h ) . % ] : 269.1 [ m - ch2 , 3.2% ] , 180.1 [ 55% ] , 179.1 [ 65% ] , 178.1 [ m - phch2 , 100% ] , 165.1 [ 30% ] , 121.0 [ 42% ] , 77.0 [ ph , 13% ] , 64.0 [ so2 , 31.7% ] : c , 55.11 ; h , 6.05 ; n , 4.94 . found : c , 55.29 ; h , 5.94 ; n , 4.86 . ( 2 )  1-(benzylsulfonyl)pyrrolidine-2-carboxylic acid , 2 l - amino acid is l - proline ; yield 1.24  g ( 92% ) ; mp 108110c ; rf = 0.84 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.464.43 ( dd , j1 = 7.2  hz , j2 = 15.76  hz , 1h , hooc - ch - ch2(a , b ) ) , 4.24 ( s , 2h , ch2-so2 ) , 3.483.45 ( t , j = 7.28  hz , 2h , n - ch2-ch2 ) , 2.46 - 2.45 ( m , 1h , cha of ch2 ) , 2.22 - 2.21 ( m , 1h , chb of ch2 ) , 2.152.09 ( quintet , j = 6.8  hz , 2h , ch2-ch2-ch2(a , b ) ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.1 ( co ) , 132.6 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 60.6 , 57.7 , 47.1 , 29.2 , 24.3  ppm . ir ( kbr ) cm : 3441 ( oh of acid ) , 2980 ( ch aromatic ) , 2828 ( ch aliphatic ) , 1728 ( c = o of cooh ) , 1620 ( c = c ) , 1219 , 1151 ( so2 two bands ) , 700 ( ar - h ) . % ] : 270.1 [ mh , 6.5% ] , 269.1 [ m , 9% ] , 179.1 [ 18.4% ] , 178.1 [ m - phch2 , 100% ] , 176.1 [ 32.4% ] , 122.0 [ 49% ] , 105.0 [ 32% ] . c12h15no4s : c , 53.52 ; h , 5.61 ; n , 5.20 . found : c , 53.77 ; h , 5.49 ; n , 5.34 . l - amino acid is l - proline ; yield 1.24  g ( 92% ) ; mp 108110c ; rf = 0.84 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.464.43 ( dd , j1 = 7.2  hz , j2 = 15.76  hz , 1h , hooc - ch - ch2(a , b ) ) , 4.24 ( s , 2h , ch2-so2 ) , 3.483.45 ( t , j = 7.28  hz , 2h , n - ch2-ch2 ) , 2.46 - 2.45 ( m , 1h , cha of ch2 ) , 2.22 - 2.21 ( m , 1h , chb of ch2 ) , 2.152.09 ( quintet , j = 6.8  hz , 2h , ch2-ch2-ch2(a , b ) ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.1 ( co ) , 132.6 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 60.6 , 57.7 , 47.1 , 29.2 , 24.3  ppm . ir ( kbr ) cm : 3441 ( oh of acid ) , 2980 ( ch aromatic ) , 2828 ( ch aliphatic ) , 1728 ( c = o of cooh ) , 1620 ( c = c ) , 1219 , 1151 ( so2 two bands ) , 700 ( ar - h ) . ms : in m / z [ rel . % ] : 270.1 [ mh , 6.5% ] , 269.1 [ m , 9% ] , 179.1 [ 18.4% ] , 178.1 [ m - phch2 , 100% ] , 176.1 [ 32.4% ] , 122.0 [ 49% ] , 105.0 [ 32% ] . : c , 53.52 ; h , 5.61 ; n , 5.20 . found : c , 53.77 ; h , 5.49 ; n , 5.34 . ( 3 )  2-(phenylmethylsulfonamido)acetic acid , 3amino acid is glycine ; yield 1.08  g ( 94% ) ; mp 150 - 151c ; rf = 0.51 . h - nmr ( d2o , 400  mhz )  : 7.45 ( s , 5h , ar - h ) , 4.19 ( s , 2h , ch2-so2 ) , 3.76 ( s , 2h , ch2-cooh ) ppm . c - nmr ( dioxane , 100  mhz )  : 170.6 ( co ) , 132.7 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 57.7 , 40.8  ppm . ir ( kbr ) cm : 3433 ( oh of acid ) , 3030 ( n  h ) , 2990 ( ch aromatic ) , 2832 ( ch aliphatic ) , 1736 ( c = o of cooh ) , 1616 ( c = c ) , 1215 , 1171 ( so2 two bands ) , 702 ( ar - h ) . % ] : 212.1 [ m - oh , 7.9% ] , 180.1 [ 73% ] , 179.1 [ 88% ] , 178.1 [ m - phch2 , 100% ] , 91.1 [ phch2 , 48% ] , 64 [ 26% ] 45 [ cooh , 2.4% ] . calcd . for 229.26 c9h11no4s : c , 47.15 ; h , 4.84 ; n , 6.11 . amino acid is glycine ; yield 1.08  g ( 94% ) ; mp 150 - 151c ; rf = 0.51 . h - nmr ( d2o , 400  mhz )  : 7.45 ( s , 5h , ar - h ) , 4.19 ( s , 2h , ch2-so2 ) , 3.76 ( s , 2h , ch2-cooh ) ppm . c - nmr ( dioxane , 100  mhz )  : 170.6 ( co ) , 132.7 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 57.7 , 40.8  ppm . ir ( kbr ) cm : 3433 ( oh of acid ) , 3030 ( n  h ) , 2990 ( ch aromatic ) , 2832 ( ch aliphatic ) , 1736 ( c = o of cooh ) , 1616 ( c = c ) , 1215 , 1171 ( so2 two bands ) , 702 ( ar - h ) . % ] : 212.1 [ m - oh , 7.9% ] , 180.1 [ 73% ] , 179.1 [ 88% ] , 178.1 [ m - phch2 , 100% ] , 91.1 [ phch2 , 48% ] , 64 [ 26% ] 45 [ cooh , 2.4% ] . anal . : c , 47.15 ; h , 4.84 ; n , 6.11 . found : c , 46.90 ; h , 5.01 ; n , 5.97 . ( 4 )  2-(phenylmethylsulfonamido)propanoic acid , 4 l - amino acid is l - alanine ; yield 1.18  g ( 97% ) ; mp 126128c ; rf = 0.81 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.22 ( s , 2h , ch2-so2 ) , 4.144.08 ( q , j = 7.28  hz , 1h , ch - ch3 ) , 1.60 - 1.58 ( d , j = 7.28  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 176.8 ( co ) , 131.0 , 130.6 ( 2ch aromatic ) , 129.3 ( 2ch aromatic ) , 129.1 , 60.4 , 52.1 , 19.6  ppm . ir ( kbr ) cm : 3424 ( oh of acid ) , 2974 ( ch aromatic ) , 2822 ( ch aliphatic ) , 1751 ( c = o of cooh ) , 1599 ( c = c ) , 1213 , 1169 ( so2 two bands ) , 698 ( ar - h ) . % ] : 212.1 [ 22% ] , 180.1 [ 81.5% ] , 179.1 [ 91% ] , 178.1 [ 85% ] , 165.1 [ m - phch3 , 55% ] , 122.0 [ 80% ] , 121.0 [ 100% ] , 77.0 [ ph , 71.4% ] , 64.0 [ so2 , 54.6% ] , 51.0 [ 28% ] . : c , 49.37 ; h , 5.39 ; n , 5.76 . found : c , 49.29 ; h , 5.28 ; n , 5.94 . l - amino acid is l - alanine ; yield 1.18  g ( 97% ) ; mp 126128c ; rf = 0.81 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.22 ( s , 2h , ch2-so2 ) , 4.144.08 ( q , j = 7.28  hz , 1h , ch - ch3 ) , 1.60 - 1.58 ( d , j = 7.28  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 176.8 ( co ) , 131.0 , 130.6 ( 2ch aromatic ) , 129.3 ( 2ch aromatic ) , 129.1 , 60.4 , 52.1 , 19.6  ppm . ir ( kbr ) cm : 3424 ( oh of acid ) , 2974 ( ch aromatic ) , 2822 ( ch aliphatic ) , 1751 ( c = o of cooh ) , 1599 ( c = c ) , 1213 , 1169 ( so2 two bands ) , 698 ( ar - h ) . ms : in m / z [ rel . % ] : 212.1 [ 22% ] , 180.1 [ 81.5% ] , 179.1 [ 91% ] , 178.1 [ 85% ] , 165.1 [ m - phch3 , 55% ] , 122.0 [ 80% ] , 121.0 [ 100% ] , 77.0 [ ph , 71.4% ] , 64.0 [ so2 , 54.6% ] , 51.0 [ 28% ] . : c , 49.37 ; h , 5.39 ; n , 5.76 . found : c , 49.29 ; h , 5.28 ; n , 5.94 . ( 5 )  3-mercapto-2-(phenylmethylsulfonamido)propanoic acid , 5l - amino acid is cysteine ; yield 1.22  g ( 89% ) ; mp 171173c ; rf = 0.49 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.50 - 4.47 ( dd , j1 = 4.24  hz , j2 = 7.92  hz , 1h , ch2-ch - cooh ) , 4.24 ( s , 2h , ch2-so2 ) , 3.553.50 ( dd , j1 = 4.24  hz , j2 = 20  hz , 1h , cha of ch2-ch ) , 3.393.33 ( dd , j1 = 7.92  hz , j2 = 20  hz , 1h , chb of ch2-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 171.5 ( co ) , 132.6 , 131.3 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.7 , 57.8 , 52.7 , 37.2  ppm . ir ( kbr ) cm : 3439 ( oh of acid ) , 2978 ( ch aromatic ) , 2832 ( ch aliphatic ) , 1728 ( c = o of cooh ) , 1618 ( c = c ) , 1207 , 1159 ( so2 two bands ) , 810 ( ar - h ) . % ] : 214.1 [ 31.7% ] , 123.0 [ 100% ] , 122.0 [ 90% ] , 92.1 [ phch3 , 33% ] , 91.0 [ phch2 , 88% ] , 77.0 [ ph , 8% ] , 65.0 [ hso2 , 34% ] , 45.0 [ cooh , 28% ] , 36.0 [ 34% ] . : c , 43.62 ; h , 4.76 ; n , 5.09 . found : c , 43.45 ; h , 4.94 ; n , 5.07 . l - amino acid is cysteine ; yield 1.22  g ( 89% ) ; mp 171173c ; rf = 0.49 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.50 - 4.47 ( dd , j1 = 4.24  hz , j2 = 7.92  hz , 1h , ch2-ch - cooh ) , 4.24 ( s , 2h , ch2-so2 ) , 3.553.50 ( dd , j1 = 4.24  hz , j2 = 20  hz , 1h , cha of ch2-ch ) , 3.393.33 ( dd , j1 = 7.92  hz , j2 = 20  hz , 1h , chb of ch2-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 171.5 ( co ) , 132.6 , 131.3 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.7 , 57.8 , 52.7 , 37.2  ppm . ir ( kbr ) cm : 3439 ( oh of acid ) , 2978 ( ch aromatic ) , 2832 ( ch aliphatic ) , 1728 ( c = o of cooh ) , 1618 ( c = c ) , 1207 , 1159 ( so2 two bands ) , 810 ( ar - h ) . % ] : 214.1 [ 31.7% ] , 123.0 [ 100% ] , 122.0 [ 90% ] , 92.1 [ phch3 , 33% ] , 91.0 [ phch2 , 88% ] , 77.0 [ ph , 8% ] , 65.0 [ hso2 , 34% ] , 45.0 [ cooh , 28% ] , : c , 43.62 ; h , 4.76 ; n , 5.09 . found : c , 43.45 ; h , 4.94 ; n , 5.07 . 
 ( 6 )  4-(methylthio)-2-(phenylmethylsulfonamido)butanoic acid , 6l - amino acid is l - methionine ; yield 1.33  g ( 87.7% ) ; mp 89 - 90c ; rf = 0.68 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.23 ( s , 2h , ch2-so2 ) , 4.013.97 ( t , j = 8.84  hz , 1h , ch - ch2 ) , 2.762.72 ( t , j = 7.40  hz , 2h , ch2-ch2-s ) , 2.332.29 ( m , 1h , ch ) , 2.282.21 ( m , 1h , ch ) , 2.19 ( s , 3h , ch3-s ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.9 ( co ) , 132.6 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.7 , 57.9 , 52.8 , 29.8 , 29.4 , 14.7  ppm . ir ( kbr ) cm : 3442 ( oh of acid ) , 2974 ( ch aromatic ) , 2833 ( ch aliphatic ) , 2774 ( ch aliphatic ) , 1742 ( c = o of cooh ) , : c , 47.51 ; h , 5.65 ; n , 4.62 . found : c , 47.49 ; h , 5.64 ; n , 4.66 . 
  l - amino acid is l - methionine ; yield 1.33  g ( 87.7% ) ; mp 89 - 90c ; rf = 0.68 . h - nmr ( d2o , 400  mhz )  : 7.47 ( s , 5h , ar - h ) , 4.23 ( s , 2h , ch2-so2 ) , 4.013.97 ( t , j = 8.84  hz , 1h , ch - ch2 ) , 2.762.72 ( t , j = 7.40  hz , 2h , ch2-ch2-s ) , 2.332.29 ( m , 1h , ch ) , 2.282.21 ( m , 1h , ch ) , 2.19 ( s , 3h , ch3-s ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.9 ( co ) , 132.6 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.7 , 57.9 , 52.8 , 29.8 , 29.4 , 14.7  ppm . ir ( kbr ) cm : 3442 ( oh of acid ) , 2974 ( ch aromatic ) , 2833 ( ch aliphatic ) , 2774 ( ch aliphatic ) , 1742 ( c = o of cooh ) , 1590 ( c = c ) , 1211 , 1161 ( so2 two bands ) , 698 ( ar - h ) . : c , 47.51 ; h , 5.65 ; n , 4.62 . found : c , 47.49 ; h , 5.64 ; n , 4.66 . ( 7 )  3-methyl-2-(phenylmethylsulfonamido)butanoic acid , 7 l - amino acid is l - valine ; yield 1.34  g ( 98.7% ) ; mp 137 - 138c ; rf = 0.83 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.25 ( s , 2h , ch2-so2 ) , 4.04 - 4.03 ( d , j = 4.4  hz , 1h , ch - ch - cooh ) , 2.472.38 ( m , 1h , ch - ch-(ch3)2 ) , 1.151.13 ( d , j = 7.04  hz , 3h , ch3-ch ) , 1.131.11 ( d , j = 7.08  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 59.2 , 57.7 , 29.8 , 18.1 , 17.8  ppm . ir ( kbr ) cm : 3447 ( oh of acid ) , 2974 ( ch aromatic ) , 2833 ( ch aliphatic ) , 2783 ( ch aliphatic ) , 1730 ( c = o of cooh ) , 1618 ( c = c ) , 1225 , 1165 ( so2 two bands ) , 700 ( ar - h ) . % ] : 271.1 [ m , 14% ] , 91.0 [ phch2 , 30% ] , 75.0 [ 65% ] , 72.1 [ 100% ] , 55.0 [ 79% ] , 29.0 [ 50% ] . : c , 53.12 ; h , 6.32 ; n , 5.16 . found : c , 53.31 ; h , 6.50 ; n , 5.20 . 
   l - amino acid is l - valine ; yield 1.34  g ( 98.7% ) ; mp 137 - 138c ; rf = 0.83 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.25 ( s , 2h , ch2-so2 ) , 4.04 - 4.03 ( d , j = 4.4  hz , 1h , ch - ch - cooh ) , 2.472.38 ( m , 1h , ch - ch-(ch3)2 ) , 1.151.13 ( d , j = 7.04  hz , 3h , ch3-ch ) , 1.131.11 ( d , j = 7.08  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 59.2 , 57.7 , 29.8 , 18.1 , 17.8  ppm . ir ( kbr ) cm : 3447 ( oh of acid ) , 2974 ( ch aromatic ) , 2833 ( ch aliphatic ) , 2783 ( ch aliphatic ) , 1730 ( c = o of cooh ) , 1618 ( c = c ) , 1225 , 1165 ( so2 two bands ) , 700 ( ar - h ) . % ] : 271.1 [ m , 14% ] , 91.0 [ phch2 , 30% ] , 75.0 [ 65% ] , 72.1 [ 100% ] , 55.0 [ 79% ] , 29.0 [ 50% ] . anal . : c , 53.12 ; h , 6.32 ; n , 5.16 . found : c , 53.31 ; h , 6.50 ; n , 5.20 . ( 8)  3-hydroxy-2-(phenylmethylsulfonamido)butanoic acid , 8l - amino acid is l - threonine ; yield 1.25  g ( 91.5% ) ; mp 194 - 195c ; rf = 0.48 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.484.46 ( m , 1h , ch ) , 4.24 ( s , 2h , ch2-so2 ) , 4.05 - 4.04 ( d , j = 4.4  hz , 1h , ch - ch - cooh ) , 1.421.41 ( d , j = 6.64  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 171.5 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 66.1 , 59.4 , 57.7 , 19.8  ppm . ir ( kbr ) cm : 3404 ( oh of acid ) , 2976 ( ch aromatic ) , 2824 ( ch aliphatic ) , 1740 ( c = o of cooh ) , 1601 ( c = c ) , 1219 , 1157 ( so2 two bands ) , 700 ( ar - h ) . : c , 48.34 ; h , 5.53 ; n , 5.12 . found : c , 48.29 ; h , 5.61 ; n , 4.98 . l - amino acid is l - threonine ; yield 1.25  g ( 91.5% ) ; mp 194 - 195c ; rf = 0.48 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.484.46 ( m , 1h , ch ) , 4.24 ( s , 2h , ch2-so2 ) , 4.05 - 4.04 ( d , j = 4.4  hz , 1h , ch - ch - cooh ) , 1.421.41 ( d , j = 6.64  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 171.5 ( co ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 66.1 , 59.4 , 57.7 , 19.8  ppm . ir ( kbr ) cm : 3404 ( oh of acid ) , 2976 ( ch aromatic ) , 2824 ( ch aliphatic ) , 1740 ( c = o of cooh ) , 1601 ( c = c ) , 1219 , 1157 ( so2 two bands ) , 700 ( ar - h ) . : c , 48.34 ; h , 5.53 ; n , 5.12 . found : c , 48.29 ; h , 5.61 ; n , 4.98 . ( 9 )  5-amino-5-oxo-2-(phenylmethylsulfonamido)pentanoic acid , 9l - amino acid is l - glutamine ; yield 1.41  g ( 94.1% ) ; mp 211214c ; rf = 0.38 . h - nmr ( d2o , 400  mhz )  : 7.48 ( s , 5h , ar - h ) , 4.474.43 ( dd , j1 = 5.04  hz , j2 = 14.32  hz , 1h , hooc - ch - ch2 ) , 4.23 ( s , 2h , ch2-so2 ) , 2.622.55 ( m , 1h , ch ) , 2.492.44 ( dd , j1 = 9.2  hz , j2 = 18.72  hz , 2h , ch2-ch2-con ) , 2.272.20 ( m , 1h , ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 181.1 ( co of acid ) , 163.2 ( co of amide ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 59.2 , 57.7 , 32.3 , 26.2  ppm . ir ( kbr ) cm : 3246 ( oh of acid ) , 3075 , 3053 ( nh two bands ) , 2983 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1703 ( c = o of cooh ) , 1659 ( c = o amide ) , 1412 ( oh bending in - plane ) , 1221 , 1193 ( so2 two bands ) , 696 ( ar - h ) , 631 ( n - h bending with wagging ) . : c , 47.99 ; h , 5.37 ; n , 9.33 . found : c , 48.03 ; h , 5.56 ; n , 9.41 . l - amino acid is l - glutamine ; yield 1.41  g ( 94.1% ) ; mp 211214c ; rf = 0.38 . h - nmr ( d2o , 400  mhz )  : 7.48 ( s , 5h , ar - h ) , 4.474.43 ( dd , j1 = 5.04  hz , j2 = 14.32  hz , 1h , hooc - ch - ch2 ) , 4.23 ( s , 2h , ch2-so2 ) , 2.622.55 ( m , 1h , ch ) , 2.492.44 ( dd , j1 = 9.2  hz , j2 = 18.72  hz , 2h , ch2-ch2-con ) , 2.272.20 ( m , 1h , ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 181.1 ( co of acid ) , 163.2 ( co of amide ) , 132.5 , 131.2 ( 2ch aromatic ) , 129.5 ( 2ch aromatic ) , 128.9 , 59.2 , 57.7 , 32.3 , 26.2  ppm . ir ( kbr ) cm : 3246 ( oh of acid ) , 3075 , 3053 ( nh two bands ) , 2983 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1703 ( c = o of cooh ) , 1659 ( c = o amide ) , 1412 ( oh bending in - plane ) , 1221 , 1193 ( so2 two bands ) , 696 ( ar - h ) , 631 ( n - h bending with wagging ) . : c , 47.99 ; h , 5.37 ; n , 9.33 . found : c , 48.03 ; h , 5.56 ; n , 9.41 . ( 10 )  3-phenyl-2-(phenylmethylsulfonamido)propanoic acid , 10 l - amino acid is l - phenyl alanine ; yield = 1.58  g ( 98.8% ) ; mp 158 - 159c ( dec ) ; rf = 0.84 . h - nmr ( d2o , 400  mhz )  : 7.46 ( s , 10h , 2  ar - h ) , 4.344.31 ( dd , j1 = 5.60  hz , j2 = 13.28  hz , 1h , phch2-ch - cooh ) , 4.22 ( s , 2h , ch2-so2 ) , 3.413.36 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , cha of ch2-ph ) , 3.283.22 ( dd , j1 = 7.60  hz , j2 = 20  hz , 1h , chb of ch2-ph ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.3 ( co of acid ) , 134.8 , 132.6 , 131.2 ( 2  ch aromatic ) , 130.3 ( 2  ch aromatic ) , 130.1 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 ( 2  ch aromatic ) , 57.7 , 55.0 , 36.4  ppm . ir ( kbr ) cm : 3441 ( oh of acid ) , 2974 ( nh ) , 2822 ( ch aromatic ) , 2776 ( ch aliphatic ) , 1740 ( c = o of cooh ) , 1609 ( c = c ) , 1221 , 1171 ( so2 two bands ) , 702 ( ar - h ) , 623 ( n - h bending ) . % ] : 270.1 [ 4% ] , 212.1 [ 12% ] , 180.1 [ 90% ] , 179.1 [ 95% ] , 178.1 [ 100% ] , 165.1 [ 60% ] , 122.0 [ 34% ] , 121.0 [ 67% ] , 64 [ so2 , 70% ] . anal . calcd . for 319.38 c16h17no4s : c , 60.17 ; h , 5.37 ; n , 4.39 . found : c , 59.98 ; h , 5.21 ; n , 4.42 . l - amino acid is l - phenyl alanine ; yield = 1.58  g ( 98.8% ) ; mp 158 - 159c ( dec ) ; rf = 0.84 . h - nmr ( d2o , 400  mhz )  : 7.46 ( s , 10h , 2  ar - h ) , 4.344.31 ( dd , j1 = 5.60  hz , j2 = 13.28  hz , 1h , phch2-ch - cooh ) , 4.22 ( s , 2h , ch2-so2 ) , 3.413.36 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , cha of ch2-ph ) , 3.283.22 ( dd , j1 = 7.60  hz , j2 = 20  hz , 1h , chb of ch2-ph ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.3 ( co of acid ) , 134.8 , 132.6 , 131.2 ( 2  ch aromatic ) , 130.3 ( 2  ch aromatic ) , 130.1 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 ( 2  ch aromatic ) , 57.7 , 55.0 , 36.4  ppm . ir ( kbr ) cm : 3441 ( oh of acid ) , 2974 ( nh ) , 2822 ( ch aromatic ) , 2776 ( ch aliphatic ) , 1740 ( c = o of cooh ) , 1609 ( c = c ) , 1221 , 1171 ( so2 two bands ) , 702 ( ar - h ) , 623 ( n - h bending ) . % ] : 270.1 [ 4% ] , 212.1 [ 12% ] , 180.1 [ 90% ] , 179.1 [ 95% ] , 178.1 [ 100% ] , 165.1 [ 60% ] , 122.0 [ 34% ] , 121.0 [ 67% ] , 64 [ so2 , 70% ] . : c , 60.17 ; h , 5.37 ; n , 4.39 . found : c , 59.98 ; h , 5.21 ; n , 4.42 . ( 11 )  3-(4-(benzylsulfonyloxy)phenyl)-2-(phenylmethylsulfonamido)propanoic acid , 11l - amino acid is tyrosine ; yield = 2.19  g ( 89.6% ) ; mp 195 - 196c ; rf = 0.82 . h - nmr ( d2o , 400  mhz )  : 7.43 ( s , 10h , 2  ar - h ) , 7.227.20 ( d , j = 8.4  hz , 2h , ots - h ) , 6.936.91 ( d , j = 8.4  hz , 2h , ots - h ) , 4.314.28 ( dd , j1 = 5.60  hz , j2 = 13.08  hz , 1h , phch2-ch - cooh ) , 4.19 ( s , 4h , 2  ch2-so2 ) , 3.31 - 3.26 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , cha of ch2-ph ) , 3.193.14 ( dd , j1 = 7.52  hz , j2 = 20  hz , 1h , chb of ch2-ph ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.8 ( co of acid ) , 156.5 , 140.9 , 132.0 , 131.8 ( 2ch aromatic ) , 131.3 ( 2  ch aromatic ) , 129.6 ( 2  ch aromatic ) , 129.0 ( 2  ch aromatic ) , 126.6 , 123.6 ( 2  ch aromatic ) , 117.1 ( 2  ch aromatic ) , 111.8 ( 2  ch aromatic ) , 58.4 , 55.4 , 36.1  ppm . ir ( kbr ) cm : 3435 ( oh of acid ) , 3167 ( nh ) , 3028 ( ch aromatic ) , 2949 ( ch aliphatic ) , 1724 ( c = o of cooh ) , 1597 ( c = c ) , 1194 , 1148 ( so2 two bands ) , 789 ( s - or ester ) , 694 ( ar - h ) , 631 ( n - h bending ) . ms : in m / z [ rel . % ] : 180.1 [ 74% ] , 179.1 [ 90% ] , 178.1 [ 100% ] , 165.1 [ 30% ] , 122.0 [ 10% ] , 91.1 [ phch2 , 47% ] , 64 [ so2 , 24% ] . : c , 56.43 ; h , 4.76 ; n , 2.86 . found : c , 56.38 ; h , 4.79 ; n , 2.69 . l - amino acid is tyrosine ; yield = 2.19  g ( 89.6% ) ; mp 195 - 196c ; rf = 0.82 . h - nmr ( d2o , 400  mhz )  : 7.43 ( s , 10h , 2  ar - h ) , 7.227.20 ( d , j = 8.4  hz , 2h , ots - h ) , 6.936.91 ( d , j = 8.4  hz , 2h , ots - h ) , 4.314.28 ( dd , j1 = 5.60  hz , j2 = 13.08  hz , 1h , phch2-ch - cooh ) , 4.19 ( s , 4h , 2  ch2-so2 ) , 3.31 - 3.26 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , cha of ch2-ph ) , 3.193.14 ( dd , j1 = 7.52  hz , j2 = 20  hz , 1h , chb of ch2-ph ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.8 ( co of acid ) , 156.5 , 140.9 , 132.0 , 131.8 ( 2ch aromatic ) , 131.3 ( 2  ch aromatic ) , 129.6 ( 2  ch aromatic ) , 129.0 ( 2  ch aromatic ) , 126.6 , 123.6 ( 2  ch aromatic ) , 117.1 ( 2  ch aromatic ) , 111.8 ( 2  ch aromatic ) , 58.4 , 55.4 , 36.1  ppm . ir ( kbr ) cm : 3435 ( oh of acid ) , 3167 ( nh ) , 3028 ( ch aromatic ) , 2949 ( ch aliphatic ) , 1724 ( c = o of cooh ) , 1597 ( c = c ) , 1194 , 1148 ( so2 two bands ) , 789 ( s - or ester ) , 694 ( ar - h ) , 631 ( n - h bending ) . ms : in m / z [ rel . % ] : 180.1 [ 74% ] , 179.1 [ 90% ] , 178.1 [ 100% ] , 165.1 [ 30% ] , 122.0 [ 10% ] , 91.1 [ phch2 , 47% ] , 64 [ so2 , 24% ] . : c , 56.43 ; h , 4.76 ; n , 2.86 . found : c , 56.38 ; h , 4.79 ; n , 2.69 . to a solution of -tolylsulfonamide derivatives 111 ( 2.96  mmol ) in h2o ( 10  ml ) in a streaming flow of nitrogen gas was added oxalyl chloride ( 0.34  ml , 3.85  mmol , 1.30 eq . ) via dropping pipette followed by carefully controlled addition of 1 drop of dmf . the resulting mixture was stirred at room temperature for 2  h to get crude acid chloride which was kept air - tighted prior to use . in a separate 250  ml three - necked round bottom flask , equipped with a magnetic stirring bar , was added na2co3 ( 0.628  g , 5.92  mmol , 2 equiv . ) to h2o ( 10  ml ) followed by diethyl amine dea ( 0.4  ml , 3.85  mmol , 1.3 equiv . ) in a continuous stirring and cooled to 15c . then , earlier kept acid chloride was added in such a way to maintain the internal temperature of the reaction mixture at around 10c . the reacting mixture was then stirred at 10c for 1  h ; at 0c for 1  h ; finally at room temperature for 1  h. the reaction was terminated , worked up by acidifying with 2  n hcl , and concentrated in a rotary evaporator . the clear solution obtained was freeze - dried to get crude solid product which was purified by column chromatography ( chcl3/ch3oh , 3  :  1 ) to afford n , n - dimethylacetamide of -tolylsulfonamide derivatives 1222 . ( 1 )  1-(benzylsulfonyl)-n , n - diethylpiperidine-2-carboxamide , 12yield 0.99  g ( 99.0% ) ; mp 210 - 211c ; rf = 0.72 . h - nmr ( d2o , 400  mhz )  : 7.54 ( s , 5h , ar - h ) , 4.30 ( s , 2h , ch2-so2 ) , 4.084.04 ( dd , j1 = 3.52  hz , j2 = 20.00  hz , 1h , cha of ch2-n ) , 3.603.57 ( m , 1h , ch - con ) , 3.213.16 ( q , j = 7.20  hz , 4h , 2  ch2-ch3 ) , 2.422.38 ( dd , j1 = 3.32  hz , j2 = 20.00  hz , 1h , chb of ch2-n ) , 2.031.99 ( m , 2h , ch2 ) , 1.861.73 ( m , 3h , ch & ch2 ) , 1.401.37 ( t , j = 7.20  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.9 , 131.1 ( 2  ch aromatic ) , 129.8 ( 2  ch aromatic ) , 128.6 , 60.6 , 57.8 , 47.4 , 43.4 ( ch2 ) , 29.4 , 24.2 , 18.1 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3028 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1720 ( c = o ) , 1593 ( c = c ) , 1188 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 60.33 ; h , 7.74 ; n , 8.28 . found : c , 60.29 ; h , 6.94 ; n , 7.98 . yield 0.99  g ( 99.0% ) ; mp 210 - 211c ; rf = 0.72 . h - nmr ( d2o , 400  mhz )  : 7.54 ( s , 5h , ar - h ) , 4.30 ( s , 2h , ch2-so2 ) , 4.084.04 ( dd , j1 = 3.52  hz , j2 = 20.00  hz , 1h , cha of ch2-n ) , 3.603.57 ( m , 1h , ch - con ) , 3.213.16 ( q , j = 7.20  hz , 4h , 2  ch2-ch3 ) , 2.422.38 ( dd , j1 = 3.32  hz , j2 = 20.00  hz , 1h , chb of ch2-n ) , 2.031.99 ( m , 2h , ch2 ) , 1.861.73 ( m , 3h , ch & ch2 ) , 1.401.37 ( t , j = 7.20  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.9 , 131.1 ( 2  ch aromatic ) , 129.8 ( 2  ch aromatic ) , 128.6 , 60.6 , 57.8 , 47.4 , 43.4 ( ch2 ) , 29.4 , 24.2 , 18.1 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3028 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1720 ( c = o ) , 1593 ( c = c ) , 1188 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 60.33 ; h , 7.74 ; n , 8.28 . found : c , 60.29 ; h , 6.94 ; n , 7.98 . ( 2 )  1-(benzylsulfonyl)-n , n - diethylpyrrolidine-2-carboxamide , 13yield 0.94  g ( 97.9% ) ; mp 185187c ; rf = 0.71 . h - nmr ( d2o , 400  mhz )  : 7.53 ( s , 5h , ar - h ) , 4.544.50 ( m , 1h , ch - con ) , 4.28 ( s , 2h , ch2-so2 ) , 3.563.54 ( m , 2h , ch2-n ) , 3.193.14 ( q , j = 7.28  hz , 4h , 2  ch2-ch3 ) , 2.542.51 ( m , 1h , ch ) , 2.282.24 ( m , 1h , ch ) , 2.162.14 ( m , 2h , ch2 ) , 1.391.35 ( t , j = 7.28  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.2 ( c = o ) , 132.6 , 131.1 ( 2  ch aromatic ) , 129.4 ( 2  ch aromatic ) , 128.8 , 60.8 , 57.7 , 47.1 , 43.3 ( 2  ch2 ) , 29.2 , 24.2 , 11.4 ( 2  ch3 ) ppm : c , 59.23 ; h , 7.46 ; n , 8.63 . found : c , 59.09 ; h , 7.46 ; n , 8.48 . h - nmr ( d2o , 400  mhz )  : 7.53 ( s , 5h , ar - h ) , 4.544.50 ( m , 1h , ch - con ) , 4.28 ( s , 2h , ch2-so2 ) , 3.563.54 ( m , 2h , ch2-n ) , 3.193.14 ( q , j = 7.28  hz , 4h , 2  ch2-ch3 ) , 2.542.51 ( m , 1h , ch ) , 2.282.24 ( m , 1h , ch ) , 2.162.14 ( m , 2h , ch2 ) , 1.391.35 ( t , j = 7.28  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.2 ( c = o ) , 132.6 , 131.1 ( 2  ch aromatic ) , 129.4 ( 2  ch aromatic ) , 128.8 , 60.8 , 57.7 , 47.1 , 43.3 ( 2  ch2 ) , 29.2 , 24.2 , 11.4 ( 2  ch3 ) ppm : c , 59.23 ; h , 7.46 ; n , 8.63 . found : c , 59.09 ; h , 7.46 ; n , 8.48 . ( 3 )  n , n - diethyl-2-(phenylmethylsulfonamido)acetamide , 14yield 0.78  g ( 92.6% ) ; mp 213215c ; rf = 0.51 . h - nmr ( d2o , 400  mhz )  : 7.53 ( s , 5h , ar - h ) , 4.29 ( s , 2h , ch2-so2 ) , 4.01 ( s , 2h , ch2-con ) , 3.20 - 3.14 ( q , j = 7.30  hz , 4h , 2  ch2-ch3 ) , 1.391.36 ( t , j = 7.30  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 174.1 ( c = o ) , 133.4 , 131.6 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.6 , 57.7 , 49.8 , 43.1 ( ch2 ) , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3217 ( n - h ) , 3036 ( ch aromatic ) , 2947 ( ch aliphatic ) , 1712 ( c = o ) , 1601 ( c = c ) , 1219 , 1194 , ( so2 two bands ) , 694 ( ar - h ) . : c , 54.91 ; h , 7.09 ; n , 9.85 . found : c , 55.13 ; h , 6.94 ; n , 10.08 . h - nmr ( d2o , 400  mhz )  : 7.53 ( s , 5h , ar - h ) , 4.29 ( s , 2h , ch2-so2 ) , 4.01 ( s , 2h , ch2-con ) , 3.20 - 3.14 ( q , j = 7.30  hz , 4h , 2  ch2-ch3 ) , 1.391.36 ( t , j = 7.30  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 174.1 ( c = o ) , 133.4 , 131.6 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.6 , 57.7 , 49.8 , 43.1 ( ch2 ) , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3217 ( n - h ) , 3036 ( ch aromatic ) , 2947 ( ch aliphatic ) , 1712 ( c = o ) , 1601 ( c = c ) , 1219 , 1194 , ( so2 two bands ) , 694 ( ar - h ) . anal . calcd . for c13h20n2o3s ( 284.38 ) : c , 54.91 ; h , 7.09 ; n , 9.85 . found : c , 55.13 ; h , 6.94 ; n , 10.08 . ( 4 )  n , n - diethyl-2-(phenylmethylsulfonamido)propanamide , 15yield 0.87  g ( 98.5% ) ; mp 238240c ; rf = 0.56 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 5h , ar - h ) , 4.26 ( s , 2h , ch2-so2 ) , 4.224.16 ( q , j = 7.28  hz , 1h , ch - ch3 ) , 3.183.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 1.651.63 ( d , j = 7.28  hz , 3h , ch3-ch ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.7 , 49.8 , 43.1 ( ch2 ) , 16.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3058 ( n - h ) , 3036 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1719 ( c = o ) , 1601 ( c = c ) , 1219 , 1196 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 56.35 ; h , 7.43 ; n , 9.39 . found : c , 56.11 ; h , 7.33 ; n , 9.28 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 5h , ar - h ) , 4.26 ( s , 2h , ch2-so2 ) , 4.224.16 ( q , j = 7.28  hz , 1h , ch - ch3 ) , 3.183.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 1.651.63 ( d , j = 7.28  hz , 3h , ch3-ch ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.7 , 49.8 , 43.1 ( ch2 ) , 16.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3058 ( n - h ) , 3036 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1719 ( c = o ) , 1601 ( c = c ) , 1219 , 1196 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 56.35 ; h , 7.43 ; n , 9.39 . found : c , 56.11 ; h , 7.33 ; n , 9.28 . ( 5 )  n , n - diethyl-3-mercapto-2-(phenylmethylsulfonamido)propanamide , 16yield 0.87  g ( 89.0% ) ; mp 198200c ; rf = 0.71 . h - nmr ( d2o , 400  mhz )  : 7.46 ( s , 5h , ar - h ) , 4.544.51 ( dd , j1 = 4.24  hz , j2 = 7.92  hz , 1h , ch2-ch - cooh ) , 4.21 ( s , 2h , ch2-so2 ) , 3.573.52 ( dd , j1 = 4.24  hz , j2 = 20.00  hz , 1h , cha of ch2-ch ) , 3.413.36 ( dd , j1 = 7.92  hz , j2 = 20.00  hz , 1h , chb of ch2-ch ) , 3.183.12 ( q , j = 7.35  hz , 4h , 2  ch2-ch3 ) , 1.371.33 ( t , j = 7.35  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.4 , 49.5 , 43.1 ( ch2 ) , 35.5 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 2997 ( ch aromatic ) , 2911 ( ch aliphatic ) , 1719 ( c = o ) , 1591 ( c = c ) , 1194 , 1144 ( so2 two bands ) , 696 ( ar - h ) . : c , 50.88 ; h , 6.71 ; n , 8.48 . found : c , 50.71 ; h , 6.99 ; n , 7.97 . 
  yield 0.87  g ( 89.0% ) ; mp 198200c ; rf = 0.71 . h - nmr ( d2o , 400  mhz )  : 7.46 ( s , 5h , ar - h ) , 4.544.51 ( dd , j1 = 4.24  hz , j2 = 7.92  hz , 1h , ch2-ch - cooh ) , 4.21 ( s , 2h , ch2-so2 ) , 3.573.52 ( dd , j1 = 4.24  hz , j2 = 20.00  hz , 1h , cha of ch2-ch ) , 3.413.36 ( dd , j1 = 7.92  hz , j2 = 20.00  hz , 1h , chb of ch2-ch ) , 3.183.12 ( q , j = 7.35  hz , 4h , 2  ch2-ch3 ) , 1.371.33 ( t , j = 7.35  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.4 , 49.5 , 43.1 ( ch2 ) , 35.5 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 2997 ( ch aromatic ) , 2911 ( ch aliphatic ) , 1719 ( c = o ) , 1591 ( c = c ) , 1194 , 1144 ( so2 two bands ) , 696 ( ar - h ) . : c , 50.88 ; h , 6.71 ; n , 8.48 . found : c , 50.71 ; h , 6.99 ; n , 7.97 . ( 6 )  n , n - diethyl-4-(methylthio)-2-(phenylmethylsulfonamido)butanamide , 17 yield 0.96  g ( 90.6% ) ; mp 170172c ; rf = 0.65 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.24 ( s , 2h , ch2-so2 ) , 4.234.20 ( t , j = 6.72  hz , 1h , ch - ch2 ) , 3.163.10 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.772.73 ( t , j = 7.40  hz , 2h , s - ch2-ch2 ) , 2.342.24 ( m , 2h , ch - ch2-ch2-s ) , 2.19 ( s , 3h , ch3-s ) , 1.351.31 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 , ( 2  ch aromatic ) , 57.7 , 49.8 , 43.1 ( ch2 ) , 30.8 , 29.5 , 16.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3028 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1722 ( c = o ) , 1620 ( c = c ) , 1200 , 1126 ( so2 two bands ) 698 ( ar - h ) . : c , 53.60 ; h , 7.31 ; n , 7.81 . found : c , 53.55 ; h , 7.22 ; n , 7.69 . 
   yield 0.96  g ( 90.6% ) ; mp 170172c ; rf = 0.65 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 5h , ar - h ) , 4.24 ( s , 2h , ch2-so2 ) , 4.234.20 ( t , j = 6.72  hz , 1h , ch - ch2 ) , 3.163.10 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.772.73 ( t , j = 7.40  hz , 2h , s - ch2-ch2 ) , 2.342.24 ( m , 2h , ch - ch2-ch2-s ) , 2.19 ( s , 3h , ch3-s ) , 1.351.31 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 , ( 2  ch aromatic ) , 57.7 , 49.8 , 43.1 ( ch2 ) , 30.8 , 29.5 , 16.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3028 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1722 ( c = o ) , 1620 ( c = c ) , 1200 , 1126 ( so2 two bands ) 698 ( ar - h ) . : c , 53.60 ; h , 7.31 ; n , 7.81 . found : c , 53.55 ; h , 7.22 ; n , 7.69 . ( 7 )  n , n - diethyl-3-methyl-2-(phenylmethylsulfonamido)butanamide , 18yield 0.94  g ( 97.3% ) ; mp 226230c ; rf = 0.69 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 5h , ar - h ) , 4.25 ( s , 2h , ch2-so2 ) , 4.00 - 3.97 ( d , j = 2.84  hz , 1h , ch - ch - con ) , 3.173.11 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.452.38 ( m , 1h , ch ) , 1.361.32 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) , 1.151.13 ( d , j = 7.00  hz , 3h , ch3-ch ) , 1.121.10 ( d , j = 7.00  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 60.8 , 57.7 , 49.8 , 43.1 ( ch2 ) , 16.2 , 15.5 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3053 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1718 ( c = o ) , 1611 ( c = c ) , 1219 , 1194 ( so2 two bands ) , 696 ( ar - h ) . : c , 58.87 ; h , 8.03 ; n , 8.58 . found : c , 59.01 ; h , 7.96 ; n , 8.61 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 5h , ar - h ) , 4.25 ( s , 2h , ch2-so2 ) , 4.00 - 3.97 ( d , j = 2.84  hz , 1h , ch - ch - con ) , 3.173.11 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.452.38 ( m , 1h , ch ) , 1.361.32 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) , 1.151.13 ( d , j = 7.00  hz , 3h , ch3-ch ) , 1.121.10 ( d , j = 7.00  hz , 3h , ch3-ch ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.8 ( c = o ) , 132.7 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 60.8 , 57.7 , 49.8 , 43.1 ( ch2 ) , 16.2 , 15.5 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3053 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1718 ( c = o ) , 1611 ( c = c ) , 1219 , 1194 ( so2 two bands ) , 696 ( ar - h ) . : c , 58.87 ; h , 8.03 ; n , 8.58 . found : c , 59.01 ; h , 7.96 ; n , 8.61 . ( 8)  n , n - diethyl-3-hydroxy-2-(phenylmethylsulfonamido)butanamide , 19 yield 0.78  g ( 80.2% ) ; mp 245c ( dec ) ; rf = 0.53 . h - nmr ( d2o , 400  mhz )  : 7.55 ( s , 5h , ar - h ) , 4.544.52 ( m , 1h , ch - ch - ch3 ) , 4.31 ( s , 2h , ch2-so2 ) , 4.094.08 ( d , j = 3.96  hz , 1h , ch - ch - con ) , 3.223.17 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.85 ( s , 1h , oh ) , 1.491.47 ( d , j = 6.60  hz , 3h , ch3-ch ) , 1.411.37 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.9 , 131.3 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 61.2 , 58.1 , 49.5 , 42.5 ( ch2 ) , 16.6 , 11.1 ( ch3 ) ppm . ir ( kbr ) cm : 3396 ( oh ) , 3030 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1720 ( c = o ) , 1601 ( c = c ) , 1221 , 1194 ( so2 two bands ) , 694 ( ar - h ) . : c , 54.86 ; h , 7.37 ; n , 8.53 . found : c , 54.71 ; h , 7.26 ; n , 8.65 . yield 0.78  g ( 80.2% ) ; mp 245c ( dec ) ; rf = 0.53 . h - nmr ( d2o , 400  mhz )  : 7.55 ( s , 5h , ar - h ) , 4.544.52 ( m , 1h , ch - ch - ch3 ) , 4.31 ( s , 2h , ch2-so2 ) , 4.094.08 ( d , j = 3.96  hz , 1h , ch - ch - con ) , 3.223.17 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.85 ( s , 1h , oh ) , 1.491.47 ( d , j = 6.60  hz , 3h , ch3-ch ) , 1.411.37 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 173.5 ( c = o ) , 132.9 , 131.3 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 61.2 , 58.1 , 49.5 , 42.5 ( ch2 ) , 16.6 , 11.1 ( ch3 ) ppm . ir ( kbr ) cm : 3396 ( oh ) , 3030 ( ch aromatic ) , 2945 ( ch aliphatic ) , 1720 ( c = o ) , 1601 ( c = c ) , 1221 , 1194 ( so2 two bands ) , 694 ( ar - h ) . : c , 54.86 ; h , 7.37 ; n , 8.53 . found : c , 54.71 ; h , 7.26 ; n , 8.65 . ( 9 )  n , n - diethyl-2-(phenylmethylsulfonamido)pentanedi - amide , 20 yield 0.98  g ( 93.2% ) ; mp 251253c ; rf = 0.58 . h - nmr ( d2o , 400  mhz )  : 7.51 ( s , 5h , ar - h ) , 4.524.48 ( dd , j1 = 5  hz , j2 = 14.32  hz , 1h , noc - ch - ch2a , b ) , 4.26 ( s , 2h , ch2-so2 ) , 3.173.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.672.60 ( m , 1h , cha of ch2 ) , 2.532.48 ( t , j = 8  hz , 2h , co - ch2-ch2 ) , 2.302.24 ( m , 1h , chb of ch2 ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 182.7 ( c = o ) , 177.1 ( c = o ) , 132.6 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.7 , 53.3 , 43.1 ( ch2 ) , 30.1 , 25.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3075 ( nh ) , 3053 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1703 ( c = o ) , 1659 ( c = o of con ) , 1601 ( c = c ) , 1221 , 1193 ( so2 two bands ) , 696 ( ar - h ) . : c , 54.06 ; h , 7.09 ; n , 11.82 . found : c , 53.95 ; h , 6.88 ; n , 12.01 . h - nmr ( d2o , 400  mhz )  : 7.51 ( s , 5h , ar - h ) , 4.524.48 ( dd , j1 = 5  hz , j2 = 14.32  hz , 1h , noc - ch - ch2a , b ) , 4.26 ( s , 2h , ch2-so2 ) , 3.173.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.672.60 ( m , 1h , cha of ch2 ) , 2.532.48 ( t , j = 8  hz , 2h , co - ch2-ch2 ) , 2.302.24 ( m , 1h , chb of ch2 ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 182.7 ( c = o ) , 177.1 ( c = o ) , 132.6 , 131.2 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 , 57.7 , 53.3 , 43.1 ( ch2 ) , 30.1 , 25.2 , 11.4 ( ch3 ) ppm . ir ( kbr ) cm : 3075 ( nh ) , 3053 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1703 ( c = o ) , 1659 ( c = o of con ) , 1601 ( c = c ) , 1221 , 1193 ( so2 two bands ) , 696 ( ar - h ) . : c , 54.06 ; h , 7.09 ; n , 11.82 . found : c , 53.95 ; h , 6.88 ; n , 12.01 . ( 10 )  n , n - diethyl-3-phenyl-2-(phenylmethylsulfonamido)propanamide , 21yield 1.00  g ( 90.2% ) ; mp 227229c ; rf = 0.70 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 10h , 2  ar - h ) , 4.394.36 ( dd , j1 = 5.60  hz , j2 = 7.60  hz , 1h , noc - ch - ch2a , b ) , 4.24 ( s , 2h , ch2-so2 ) , 3.443.39 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , 1h , cha of ch2a , b ) , 3.313.26 ( dd , j1 = 7.60  hz , j2 = 20.00  hz , 1h , chb of ch2a , b ) , 3.163.10 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.78 ( s , 2h , ch2 ) , 1.351.31 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( c = o ) , 135.1 , 132.7 , 131.2 ( 2  ch aromatic ) , 130.3 ( 2  ch aromatic ) , 130.1 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 ( 2  ch aromatic ) , 57.8 , 55.2 , 43.1 ( ch2 ) , 36.5 , 11.5 ( ch3 ) ppm . ir ( kbr ) cm : 2976 ( nh ) , 2828 ( ch aromatic ) , 2774 ( ch aliphatic ) , 1736 ( c = o ) , 1620 ( c = c ) , 1206 , 1153 , 1148 ( so2 two bands ) , 698 ( ar - h ) . : c , 64.14 ; h , 7.00 ; n , 7.48 . found : c , 64.00 ; h , 6.84 ; n , 7.29 . h - nmr ( d2o , 400  mhz )  : 7.49 ( s , 10h , 2  ar - h ) , 4.394.36 ( dd , j1 = 5.60  hz , j2 = 7.60  hz , 1h , noc - ch - ch2a , b ) , 4.24 ( s , 2h , ch2-so2 ) , 3.443.39 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , 1h , cha of ch2a , b ) , 3.313.26 ( dd , j1 = 7.60  hz , j2 = 20.00  hz , 1h , chb of ch2a , b ) , 3.163.10 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 2.78 ( s , 2h , ch2 ) , 1.351.31 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( c = o ) , 135.1 , 132.7 , 131.2 ( 2  ch aromatic ) , 130.3 ( 2  ch aromatic ) , 130.1 ( 2  ch aromatic ) , 129.5 ( 2  ch aromatic ) , 128.9 ( 2  ch aromatic ) , 57.8 , 55.2 , 43.1 ( ch2 ) , 36.5 , 11.5 ( ch3 ) ppm . ir ( kbr ) cm : 2976 ( nh ) , 2828 ( ch aromatic ) , 2774 ( ch aliphatic ) , 1736 ( c = o ) , 1620 ( c = c ) , 1206 , 1153 , 1148 ( so2 two bands ) , 698 ( ar - h ) . : c , 64.14 ; h , 7.00 ; n , 7.48 . found : c , 64.00 ; h , 6.84 ; n , 7.29 . 
 ( 11 )  4-(3-(diethylamino)-3-oxo-2-(phenylmethylsulfonamido)propyl)phenyl phenyl methanesulfonate , 22yield 1.44  g ( 89.3% ) ; mp 265c ( dec ) ; rf = 0.69 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 10h , 2  ar - h ) , 7.297.27 ( d , j = 8.00  hz , 2h , ar - h ) , 6.996.97 ( d , j = 8.00  hz , 2h , ar - h ) , 4.374.33 ( dd , j1 = 5.60  hz , j2 = 7.60  hz , 1h , noc - ch - ch2a , b ) , 4.26 ( s , 2h , ch2-so2 ) , 3.373.32 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , 1h , cha of ch2a , b ) , 3.263.21 ( dd , j1 = 7.60  hz , j2 = 20.00  hz , 1h , chb of ch2a , b ) , 3.173.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( c = o ) , 155.9 , 141.3 , 131.8 ( 2  ch aromatic ) , 131.3 ( 2  ch aromatic ) , 129.6 ( 2  ch aromatic ) , 129.0 ( 2  ch aromatic ) , 126.6 , 123.0 ( 2  ch aromatic ) , 116.9 ( 2  ch aromatic ) , 111.6 ( 2  ch aromatic ) , 57.8 , 55.2 , 43.2 ( ch2 ) , 35.6 , 11.6 ( ch3 ) ppm . ir ( kbr ) cm : 3058 ( nh ) , 3036 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1719 ( c = o ) , 1601 ( c = c ) , 1219 , 1196 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 59.54 ; h , 5.92 ; n , 5.14 . found : c , 59.43 ; h , 5.99 ; n , 4.98 . yield 1.44  g ( 89.3% ) ; mp 265c ( dec ) ; rf = 0.69 . h - nmr ( d2o , 400  mhz )  : 7.50 ( s , 10h , 2  ar - h ) , 7.297.27 ( d , j = 8.00  hz , 2h , ar - h ) , 6.996.97 ( d , j = 8.00  hz , 2h , ar - h ) , 4.374.33 ( dd , j1 = 5.60  hz , j2 = 7.60  hz , 1h , noc - ch - ch2a , b ) , 4.26 ( s , 2h , ch2-so2 ) , 3.373.32 ( dd , j1 = 5.60  hz , j2 = 20.00  hz , 1h , cha of ch2a , b ) , 3.263.21 ( dd , j1 = 7.60  hz , j2 = 20.00  hz , 1h , chb of ch2a , b ) , 3.173.12 ( q , j = 7.32  hz , 4h , 2  ch2-ch3 ) , 1.371.33 ( t , j = 7.32  hz , 6h , 2  ch3-ch2 ) ppm . c - nmr ( dioxane , 100  mhz )  : 172.4 ( c = o ) , 155.9 , 141.3 , 131.8 ( 2  ch aromatic ) , 131.3 ( 2  ch aromatic ) , 129.6 ( 2  ch aromatic ) , 129.0 ( 2  ch aromatic ) , 126.6 , 123.0 ( 2  ch aromatic ) , 116.9 ( 2  ch aromatic ) , 111.6 ( 2  ch aromatic ) , 57.8 , 55.2 , 43.2 ( ch2 ) , 35.6 , 11.6 ( ch3 ) ppm . ir ( kbr ) cm : 3058 ( nh ) , 3036 ( ch aromatic ) , 2951 ( ch aliphatic ) , 1719 ( c = o ) , 1601 ( c = c ) , 1219 , 1196 , 1148 ( so2 two bands ) , 696 ( ar - h ) . : c , 59.43 ; h , 5.99 ; n , 4.98 . the antimicrobial properties of the sulfonamides were investigated in the form of the general sensitivity testing and minimum inhibitory concentration ( mic ) with respect to freshly cultured targeted organisms . the two organisms of interest in this present study are one gram positive ( staphylococcus aureus atcc 6538 ) and one gram negative ( escherichia coli atcc 25922 ) organisms which are associated with the gastrointestinal tract damage in man and animal . the standard strains of s. aureus and e. coli used were obtained from test center of antimicrobial materials , tipc , beijing . no clinically isolated organism was used based on inavailability of such as at the time of this study . the strains were propagated on nutrient agar plates and maintained on the plate at 4c . the isolates were subcultured in nutrient broth at 37c for 8  h prior to antibacterial testing . agar well diffusion technique as described by adeniyi and coworkers was used to determine the antibacterial activity of the synthesized compounds . sensitivity test agar plates were seeded with 0.1  ml of an overnight culture of each bacterial strain ( equivalent to 1010 cfu  ml ) . the seeded plates were allowed to set and a standard cork borer of 8  mm diameter was used to cut uniform wells on the surface of the agar . the wells were then filled with 0.3  ml of each sulfonamide solution in an appropriate solvent at a concentration of 1000  g / ml ( 0.02  g of sulfonamide dissolved in 20  ml distilled water ) . all the plates were incubated at 37c for 24  h. the assay was conducted at regular intervals of 24  h until a marked decline in the potency of the sulfonamide solution to inhibit the growth of the test organisms was noticed . zones of clearance round each well means inhibition and the diameter of such zones were measured . agar well dilution method as described by russell and furr was used to determine the minimum inhibitory concentration ( mic ) of the sulfonamides and streptomycin . different dilutions of the sulfonamides were prepared first at 100  g / ml to give final concentrations in the range of 100 , 50 , 25 , 12.5 , 6.25 , and 1.8  g / ml . the different dilutions of sulfonamide derivatives that could not inhibit the microbial growth at 100  g / ml were later prepared at 1000  g / ml to give final concentrations in the range of 1000 , 500 , 250 , 125 , and 62.5  g / ml . two milliliter ( 2  ml ) of each dilution was mixed with 18  ml of mueller hinton agar ( mha , difco , france ) and poured into petri dishes and allowed to set . the agar was streaked with an overnight broth culture of the bacterial strains and incubated overnight . the minimum concentration that completely inhibited macroscopic growth was regarded as the minimum inhibitory concentration of the respective sulfonamide . selectivity index ( si ) is the ratio of the zone of the inhibition of compound to that of the streptomycin .
sulfonamide drugs which have brought about an antibiotic revolution in medicine are associated with a wide range of biological activities . 
 we have synthesized a series of -tolylsulfonamide , 111 and their substituted n , 
 n - diethyl-2-(phenylmethylsulfonamido ) alkanamide derivatives , 1222 in improved and excellent yields in aqueous medium at room temperature through highly economical synthetic routes . 
 the chemical structures of the synthesized compounds 122 were confirmed by analytical and spectral data such as ir , 1h- and 13c - nmr , and mass spectra . 
 the in vitro antibacterial activity of these compounds along with standard clinical reference , streptomycin , was investigated on two key targeted organisms . 
 it was observed that 1-(benzylsulfonyl)pyrrolidine-2-carboxylic acid , 2 emerged as the most active compound against staphylococcus aureus at mic value of 1.8  g / ml while 4-(3-(diethylamino)-3-oxo-2-(phenylmethylsulfonamido ) propyl)phenyl phenylmethanesulfonate , 22 was the most active sulfonamide scaffold on escherichia coli at mic value of 12.5  g / ml .