oral submucous fibrosis ( osmf ) is a chronic , premalignant condition of the oral mucosa , which was first described by schwartz in 1952 . pindborg ( 1966 ) defined osmf as ,  an insidious , chronic disease affecting any part of the oral cavity and sometimes the pharynx . although occasionally preceded by and/or associated with vesicle formation , it is always associated with juxta - epithelial inflammatory reaction followed by a fibroelastic change of the lamina propria , with epithelial atrophy leading to stiffness of the oral mucosa and causing trismus and inability to eat . it is predominantly seen in southeast asia and indian subcontinent with few cases reported from south africa , greece and united kingdom . the importance of this disease lies in its inability to open the mouth and the highest malignant transformation rate . although , the etiological factor in the causation of osmf is believed to be multifactorial , areca nut plays an important role in the disease manifestation . the chronic irritation caused by consumption of areca nut in the form of pan masala , gutkha causes injury thereby leading to chronic inflammation , cytokine production and development of oxidative stress . oxidative stress and subsequent reactive oxygen species ( ros ) generation can induce cell proliferation , cell senescence or apoptosis , depending upon the level of ros production . accumulation of genetic alterations is the basis for the progression of a normal cell to a cancerous cell . the newly forming cells would need many basic components well above the normal limits , used in physiological processes . they are major cell membrane components essential for various biological functions including cell growth and division of normal and malignant tissues . as the neoplastic disease is related to new growth , there is a greater utilization of lipids , including total cholesterol ( tc ) , lipoproteins and triglycerides ( tgs ) for new membrane biogenesis . cells fulfill these requirements either from circulation or from degradation of major lipoprotein fractions such as very low - density lipoprotein ( vldl ) , low - density lipoprotein ( ldl ) or high - density lipoprotein ( hdl ) . the usefulness of variations in plasma or serum lipid levels in the diagnosis and treatment of various diseases such as breast cancer , colorectal cancer , heart diseases has been studied by several workers . however , there have been only few studies that correlate the association of plasma or serum lipids with head and neck lesions . a careful search of the literature did not reveal any research based on comparison of lipid profile with the clinical staging and histopathological grading of osmf . a study was conducted on 50 clinically diagnosed and histopathologically proven patients of osmf attending the outpatient department , after obtaining the institutional ethical committee clearance . case proforma including patient 's consent was made to record signs , symptoms , detailed history , including habits and extended lipid profile estimation . the osmf cases were staged clinically according to lai et al . and were graded histopathologically according to utsunomiya h , et al . the control group consisted of 50 normal subjects without any pernicious oral habits within similar age groups . serum levels of tc , tg , hdl , vldl and ldl were calculated by using the urban kits in erba chem  5 plus analyzer ( transasia bio medicals pvt ltd . ) . a total of 50 osmf cases were included in the study . of 50 osmf patients , according to clinical staging , group a were 16 cases ( 32% ) , group b - 15 cases ( 30% ) , group c - 13 cases ( 26% ) and group d - 6 cases ( 12% ) , respectively [ figure 1 ] . the cases were graded histopathologically as early stage - 15 cases ( 30% ) , intermediate stage - 19 cases ( 38% ) and - advanced stage - 16 cases ( 32% ) [ figure 2 ] . distribution of oral submucous fibrosis samples by clinical staging distribution of oral submucous fibrosis samples by histological grading it was observed that the mean tc levels [ table 1 ] , hdl levels [ table 2 ] and ldl levels [ table 3 ] , of osmf patients were significantly reduced as compared with controls with a statistically significant p value of 0.00001 using student 's t - test . comparison of osmf group and control group with respect to tc comparison of osmf group and control group with respect to hdl comparison of osmf group and control group with respect to ldl it was noted that there was no change in the mean value of tgs [ table 4 ] and vldl [ table 5 ] among osmf patients as compared with controls with an insignificant p value of 1 using student 's t - test . comparison of osmf group and control group with respect to tg comparison of osmf group and control group with respect to vldl the mean tc [ figure 3 ] and hdl [ figure 4 ] levels showed a progressive decrease , as the clinical stage of osmf advances with a statistically significant p value of 0.0163 , 0.0221 by using one - way anova test . comparison of clinical staging with respect to total cholesterol levels comparison of clinical staging with respect to high - density lipoprotein levels the mean tg [ figure 5 ] , vldl [ figure 6 ] and ldl [ figure 7 ] levels did not exhibit any significant variation in different groups of osmf by using one - way anova test . comparison of clinical staging with respect to triglyceride levels comparison of clinical staging with respect to very low - density lipoprotein levels comparison of clinical staging with respect to low - density lipoprotein levels the mean tc [ figure 8 ] , tg [ figure 9 ] , hdl [ figure 10 ] , vldl [ figure 11 ] and ldl [ figure 12 ] levels showed a progressive decrease as the histological grade of osmf advanced with a statistically significant p value of 0.00001 , 0.00001 , 0.00001 , 0.00001 and 0.00001 , respectively by using one - way anova test . comparison of histological grading with respect to total cholesterol levels comparison of histological grading with respect to triglyceride levels comparison of histological grading with respect to high - density lipoprotein levels comparison of histological grading with respect to very low - density lipoprotein levels comparison of histological grading with respect to low - density lipoprotein levels it was observed that the mean tc levels [ table 1 ] , hdl levels [ table 2 ] and ldl levels [ table 3 ] , of osmf patients were significantly reduced as compared with controls with a statistically significant p value of 0.00001 using student 's t - test . comparison of osmf group and control group with respect to tc comparison of osmf group and control group with respect to hdl comparison of osmf group and control group with respect to ldl it was noted that there was no change in the mean value of tgs [ table 4 ] and vldl [ table 5 ] among osmf patients as compared with controls with an insignificant p value of 1 using student 's t - test . comparison of osmf group and control group with respect to tg comparison of osmf group and control group with respect to vldl the mean tc [ figure 3 ] and hdl [ figure 4 ] levels showed a progressive decrease , as the clinical stage of osmf advances with a statistically significant p value of 0.0163 , 0.0221 by using one - way anova test . comparison of clinical staging with respect to total cholesterol levels comparison of clinical staging with respect to high - density lipoprotein levels the mean tg [ figure 5 ] , vldl [ figure 6 ] and ldl [ figure 7 ] levels did not exhibit any significant variation in different groups of osmf by using one - way anova test . comparison of clinical staging with respect to triglyceride levels comparison of clinical staging with respect to very low - density lipoprotein levels comparison of clinical staging with respect to low - density lipoprotein levels the mean tc [ figure 8 ] , tg [ figure 9 ] , hdl [ figure 10 ] , vldl [ figure 11 ] and ldl [ figure 12 ] levels showed a progressive decrease as the histological grade of osmf advanced with a statistically significant p value of 0.00001 , 0.00001 , 0.00001 , 0.00001 and 0.00001 , respectively by using one - way anova test . comparison of histological grading with respect to total cholesterol levels comparison of histological grading with respect to triglyceride levels comparison of histological grading with respect to high - density lipoprotein levels comparison of histological grading with respect to very low - density lipoprotein levels comparison of histological grading with respect to low - density lipoprotein levels osmf has been identified as a precancerous condition with the highest rate of malignant transformation amongst potentially malignant disorders . chewing betel quid carcinogens in these substances generate ros and lipid peroxides thereby leading to tissue injury as a result of elevated lipid peroxidation , further damaging the cellular structural block , namely lipids . hence , the present study was aimed to evaluate the lipid profile in osmf patients . as compared to controls , osmf patients showed a significant reduction in the levels of tc , hdl and ldl whereas no statistically significant difference was found in the values of tg and vldl between osmf and control group . in the present study , serum lipid profile in osmf patients was compared with respect to clinical staging and the results of our study showed a statistically significant reduction in levels of tc and hdl as the clinical stage of the osmf advances . on contrary , no difference was found in the values of tg , vldl and ldl as the staging advances . finally , in the present study , the serum lipid profile in osmf patients was also compared with histological grading . after statistical evaluation , it was found that a significant reduction in the levels of tc , tg , hdl , vldl and ldl was found as the histological grade of the osmf advances . high activity of the ldl receptors attributes for lowering the serum cholesterol levels . in the present study , a significant decrease was observed in serum cholesterol and ldl levels in osmf patients , which might be a consequence of a disease that is mediated by utilization of cholesterol for membrane biogenesis . our study results coincided with the studies done by kumar et al . , in which they showed a significant decrease in tc , hdl and ldl in patients with osmf as compared to the controls . , observed a significant decrease in serum cholesterol and ldl in osmf patients , coinciding with the results of our study . however , in contrast , they also observed a significantly increased tg levels in osmf , which was not observed in our study . , found a significant decrease in plasma tc , hdl with no significant change in the levels of ldl and tgs in osmf patients as compared to controls whereas , ldl levels was found to be decreased in the present study . a study by sharma et al . , observed a significant decrease in serum cholesterol , ldl in osmf patients , which was similar to the present study . in contrast to this study , they also observed increased levels of hdl , which was decreased in our study . gupta and gupta , observed a significant decrease in plasma tc and hdl in patients with the precancerous lesions and conditions as compared to the controls similar to our study . in contrast to the present study , they also found a significant decrease in tgs . in contrast to our study goyal et al . found no statistically significant changes in the lipid profile parameters , namely tc , tgs , hdl , vldl and ldl in cases of oral precancerous lesions and conditions as compared to controls . an extensive search of english literature revealed that the present study was the first study done to evaluate the lipid profile in osmf patients with respect to both clinical staging and histological grading . our study results exhibited a significant decrease in all the lipid parameters , namely tc , tg , hdl , vldl and ldl in osmf patients with respect to histological grading as compared to clinical staging . the results of all parameters with respect to lipid profile tested in our study are not in agreement with few above - mentioned studies done on osmf patients . this may be due to differences between the studies when various parameters are considered individually . this variability in the values of lipid profiles in osmf may be due to multiple reasons such as age , nutritional status , body mass index , alcohol consumption and exercise habits . the tc , hdl and ldl levels in osmf patients were reduced as compared with controls , suggesting that a lower serum lipid profile is a useful indicator for initial changes occurring in the cells of potentially malignant disorders like osmfthe tc and hdl levels were decreased as the clinical stage of osmf advances indicating their role as a reliable biochemical indicatorall the lipid profile parameters such as tc , tg , hdl , vldl and ldl were gradually reduced as the histological grade advanced signifying their role as the best prognostic biochemical indicator . the tc , hdl and ldl levels in osmf patients were reduced as compared with controls , suggesting that a lower serum lipid profile is a useful indicator for initial changes occurring in the cells of potentially malignant disorders like osmf the tc and hdl levels were decreased as the clinical stage of osmf advances indicating their role as a reliable biochemical indicator all the lipid profile parameters such as tc , tg , hdl , vldl and ldl were gradually reduced as the histological grade advanced signifying their role as the best prognostic biochemical indicator .
background : lipids play a key role in the maintenance of cell integrity . 
 these are major cell membrane components essential for various biological functions , including cell growth and division of normal and malignant tissues . 
 abnormal changes have long been associated with alterations in lipid profile.aim:the aim of this study was to evaluate the alterations in lipid profile in oral submucous fibrosis ( osmf ) patients and to compare the levels with respect to the clinical staging and histological grading.materials and methods : fifty patients of osmf , diagnosed clinically and histopathologically , were included as the study subjects . 
 a group of 50 age and sex matched normal subjects without any oral pernicious habits were taken as controls . 
 the serum lipid profile consisting of total cholesterol ( tc ) , triglycerides ( tgs ) , high density lipoprotein ( hdl ) , very low density lipoprotein ( vldl ) and low density lipoprotein ( ldl ) were analyzed using erba chem-5 plus analyzer.results:serum tc , hdl and ldl levels were significantly decreased in osmf patients as compared to controls . 
 as the clinical stage progresses , the tc and hdl levels were gradually reduced . 
 all the lipid profile parameters such as tc , tg , hdl , vldl and ldl progressively reduced as the histological grade advanced.conclusion:the results of the present study showed that there is an inverse relationship between lipid profile and the presence of osmf . 
 the decreased serum lipid profile may be considered as a useful indicator for initial changes occurring in the cells of potentially malignant disorders like osmf .