chemical antibacterial plaque inhibitors are successfully used as mechanical oral hygiene procedures for providing supragingival cleaning . however , in long term use of the antibacterial chemicals like chlorhexidine have side effects such as bad taste and tooth discoloration . therefore , there is still a need for an antiplaque agent that can be convenient for per day usage and with minimal side - effects . antibacterial agents and certain natural products containing medical compounds are used in alternative medicine as therapeutic agents . certain substances isolated from these products have many potential effects beyond their antimicrobial activity in the treatment , and prevention of periodontal disease has been shown . among these arab acaci , eucalyptus extract , mastic , miswak it can be also used as an antibacterial , antiviral , antifungal , antioxidant and anti - inflammatory agent in homeopathic and herbal health applications , and does not have any side effects . propolis applications have been shown to be useful in treating oral ulcers and gingivitis in many cases and clinical pilot study . propolis extract has antimicrobial activity against facultative anaerobes , streptococcus mutans , and gram - positive cocci that can be found in the human oral cavity . besides , a study evaluating the activity of propolis against periodontal pathogens have shown that propolis may be of clinical value . to prevent tooth decay and treat gingivitis and stomatitis , it can be used as an active agent in mouthwash and toothpaste . in certain studies , dentifrices with propolis have been shown to have excellent plaque removal , plaque prevention , and anti - inflammatory effects propolis mouthwash can be an alternative to chemical rinse and when used in subgingival irrigation better improvement of microbiological and clinical parameters are obtained when compared with conventional treatment have been shown . in general , chemical agents are used in toothpastes , mouthwashes , and gels . however , in some cases , the use of mouthwashes and toothpastes are difficult . therefore , different tools are required for oral care . chewing gums containing antiplaque agents have been tested as an additional tools for daily oral care . steinberg et al . had been studied the release of the chlorhexidine from chewing - gum and showed that chewing gums may be an appropriate vehicle for the release of antiplaque agents . in many countries , chewing gum regularly practiced by many people as a habit and this application indirect effects of chewing gum on oral health are saliva flow stimulation and mechanical tooth cleaning . in the light of this information , it can be considered that the propolis gum might be a good tool to prevent gingival inflammation and plaque accumulation . so far , no study has used propolis gum and compared the efficacy of mouthwash with chewing - gum that both including propolis . the purpose of this study was to evaluate the effect of propolis gum compared to propolis - containing mouthwash on gingival inflammation and plaque accumulation on patients that refrain from daily oral hygiene procedures for 5 days . this study was planned as a randomized , single - blind , crossover , 5-day with 3-day washout period clinical study . approval for this study was obtained from the ethical committee for clinical research , kirikkale university , school of dentistry . 10 systemically healthy , nonsmoker college students with very good oral hygiene and gum health without any medical history were included in the study . commercially available propolis - containing gum ( bio bee propolis , biohayat , turkey ) provides 0.5% propolis extract , % 10 chestnut honey , % 1 cinnamon . the clinical status of all participants was recorded , and plaque index and gingival index have been taken immediately and after each trial period . at the same time , all participants also had received professional teeth cleaning containing scaling , polishing , and floss applications . participants randomly divided into two groups including propolis chewing - gum and propolis mouthwash group . participants were advised to refrain from any of the oral hygiene procedures ( such as brushing teeth , dental floss ) for 5-day . and then participants in chewing - gum group were instructed to use chewing gums 3 times after every meal for 20 min ( a total of 1 h / day ) , and in mouthwash group participants were told to use propolis mouthwash 2 times a day for 1 min . at the end of the day 5 , participants came to the clinic and plaque index and gingival index were taken . plaque accumulation was assessed on the facial and lingual aspects of all teeth following disclosure with erythrosine dye by a single investigator ( ne ) . after that , 3-day washout period was conducted , in this period participants were applied normal hygiene procedures , following the wash - out period , professional teeth cleaning applications were performed to the participants to obtain zero plaque index scores and the procedure performed in the first period was changed with the other alternative procedure . at day 13 , final examination , similar to that performed at day 5 , took place . before and after each experiment period professional teeth cleaning was performed to participants , and clinical periodontal measurements were recorded . wilcoxon signed rank test was also used in variables found significant differences for determining the groups leading to differences . , chicago , ill , usa ) and microsoft office excel 2007 programs were used for statistical analysis . approval for this study was obtained from the ethical committee for clinical research , kirikkale university , school of dentistry . 10 systemically healthy , nonsmoker college students with very good oral hygiene and gum health without any medical history were included in the study . commercially available propolis - containing gum ( bio bee propolis , biohayat , turkey ) provides 0.5% propolis extract , % 10 chestnut honey , % 1 cinnamon . before starting work , the clinical status of all participants was recorded , and plaque index and gingival index have been taken immediately and after each trial period . at the same time , all participants also had received professional teeth cleaning containing scaling , polishing , and floss applications . participants randomly divided into two groups including propolis chewing - gum and propolis mouthwash group . participants were advised to refrain from any of the oral hygiene procedures ( such as brushing teeth , dental floss ) for 5-day . and then participants in chewing - gum group were instructed to use chewing gums 3 times after every meal for 20 min ( a total of 1 h / day ) , and in mouthwash group participants were told to use propolis mouthwash 2 times a day for 1 min . at the end of the day 5 , participants came to the clinic and plaque index and gingival index were taken . plaque accumulation was assessed on the facial and lingual aspects of all teeth following disclosure with erythrosine dye by a single investigator ( ne ) . after that , 3-day washout period was conducted , in this period participants were applied normal hygiene procedures , following the wash - out period , professional teeth cleaning applications were performed to the participants to obtain zero plaque index scores and the procedure performed in the first period was changed with the other alternative procedure . at day 13 , final examination , similar to that performed at day 5 , took place . before and after each experiment period professional teeth cleaning was performed to participants , and clinical periodontal measurements wilcoxon signed rank test was also used in variables found significant differences for determining the groups leading to differences . , chicago , ill , usa ) and microsoft office excel 2007 programs were used for statistical analysis . of the 10 selected subjects , 5 were males and 5 females , ( age range 1822 years ) all completed the study satisfactorily . all subjects were successfully allocated to the treatments , and there was no drop - out during the study . mean full mouth plaque indexes and gingival indexes were used as the main outcome variables . during the study , no side effects from using the chewing gum or mouthwash were reported by the participants . there was a significant difference in the both plaque and gingival indexes between the groups . the plaque index of the mouthwash group was significantly lower than that of the chewing - gum group ( p = 0.005 ) and also the same results were also detectable for the gingival index in mouthwash group compared with chewing - gum group . summary statistics ( median , interquartile range ) of plaque and gingival indices for the propolis mouthwash and chewing - gum groups at day 5 are presented in table 1 . the primary objective of this study was to evaluate the effects of propolis gum and mouthwash on de novo plaque formation on tooth surfaces and gingival inflammation over a 5-day period without mechanical oral hygiene following random use of either chewing - gum or mouthwash . the degree of plaque accumulation and gingival inflammation was compared between groups ( mouthwash and chewing - gum ) ( n = 10 ) and significantly lower plaque and gingival indices were measured after propolis mouthwash use compared to the values obtained with the propolis gum . a randomized , cross - over , single - blind design was chosen in order to avoid differences in response to the treatment by different individuals and the sample size used in this study was similar to other studies which have similar purposes like our study . evidence shows that the mechanical removal of supragingival plaque is the most effective method for the prevention of the periodontal diseases . it was demonstrated that the majority of patients have not got a sufficient motivation and skills for the usage of the oral hygiene products . because of the difficulty to ensure adequate removal of plaque by mechanical means , a need exists for adjunctive methods of mechanical plaque removal that are simple , inexpensive , and convenient for usage by the patients . the major problem of these substances is the relative short contact time between the active agent and the teeth and the existence of negative side effects . among the various natural products , propolis has received greater attention due to its broad - spectrum antimicrobial activity against a wide range of pathogenic microorganisms . the mechanism of this inhibition is unknown but is thought to be related to the flavonoid content and cinamic acid of the product . propolis not only has a strong antibacterial , antifungal , antiviral , antioxidant action , but also has immunity enhancing , pain and inflammation relieving , and wound repair accelerating effects . propolis plays a role as an anti - inflammatory agent by inhibiting prostaglandin synthesis , activating the thymus gland , supporting the immune system by increasing phagocytic activity , inducing cellular immunity and enhancing healing effects on epithelial tissue . for this reason , it can be considered as a promising agent for the treatment or prevention of periodontal diseases . although there is a great interest in the use of antimicrobial agents to replace or to be adjuncts to the mechanical approaches with which carriers , they will be delivered to the oral environment is not clear . the inability of the active product to be retained locally for a sufficient period is still a challenge . it was stated that the effect of the most commonly used agent in formulations chlorhexidine is related to its release from the formulation . the formulations that release bactericides such as antibacterial varnishes and resins have an intrinsic disadvantage in that the effect is transient and greatly diminishes over time . there have been many studies about the gum use as a tool for the transport of the medical substances . the advantage of the use of chewing gum as a tool for drug delivery is it can be used ever , in any place without water and good stabilization of the product by the protection the content of the therapeutic agent from the oxygen , light and water . gum causes both local ( local delivery ) and systemic effect by swallowing or absorption of the active agent through the oral mucosa . and also the advantage of the gum instead of the mouth rinse is staying in mouth more longer times and thus lower doses of agents in the gum could be a release for extended periods . hitherto , there has not been any work that compares the effect of mouthwash and chewing - gum containing propolis on periodontal parameters . this is the first study that has been compared the two different vehicles containing propolis in preventing plaque formation and gingival inflammation . propolis extract was used in various forms but in which form its mechanism of action will more effective in periodontal treatment is unknown . in the present study , it seems that the mouthwash form is more effective than chewing - gum on the plaque and gingival indexes . however , the other studies that compared the mouthwash and the chewing - gum obtained opposite results with our study . . showed that the use of chlorhexidine in the chewing - gum was as effective in plaque inhibition as in the use of chlorhexidine ( 0.2% ) mouthwash 2 times a day , in another study that obtained the similar result was demonstrated that chlorhexidine chewing gum used with normal tooth cleaning provides similar adjunctive benefits to oral hygiene and gingival health as a 0.2% chlorhexidine rinse . but , it should be emphasized that different agent was used in these studies unlike with our study and also our finding can be explained with the onset release of the propolis from the chewing - gum that may take longer than 20 min ( the time participants chewed the gum ) so that propolis in the gum failed to show its effect . according to the results of this study , it may be speculated that the propolis mouthwash possesses better antiplaque and antigingivitis effect than propolis gum . this result is compatible with previous studies investigated the effect of the propolis mouthwash suggested that it might be used as a therapeutic , and alternative to chemical mouthwashes . our data show that the propolis mouthwash is more effective than propolis gum on plaque accumulation and gingival inflammation . but further studies are needed to evaluate the efficacy of this gum formulation that will be chewed for more prolonged time as an adjunct to mechanical plaque control , both on plaque and gingivitis and propolis may be compared with other agents and also in different vehicles .
objective : in general , chemical plaque agents have been used in mouthwashes , gels , and dentifrices . in some situations , application of mouthwashes and dentifrices can be difficult . 
 therefore , different approaches for oral health - care have been needed . 
 the aim of this study was to evaluate the effect of propolis chewing - gum compared to propolis - containing mouthwash on gingival inflammation and plaque accumulation on patients that refrained from daily oral hygiene procedures for 5 days.materials and methods:10 college students with systemically healthy and very good oral hygiene and gingival health were included in this randomized , single - blind , crossover 5-day plaque regrowth with a 3-day washout period clinical study . 
 after plaque scores were reduced to zero , participants were asked to refrain from oral hygiene procedures and allocated to either propolis mouthwash or chewing - gum group . 
 chewing - gum was performed after meals 3 times a day for 20 min mouthwash group was instructed to rinse mouthwash 2 times a day for 1 min . on day 5 , the clinical periodontal measurements containing plaque and gingival indexes were taken from the participants.results:the both plaque and gingival indexes of propolis mouthwash group were significantly lower than that of the propolis chewing - gum group ( p = 0.005).conclusion : it was demonstrated that the propolis mouthwash was more effective than the propolis chewing gum on the plaque inhibition and the gingival inflammation .