nanotechnology and science are directed toward processes , materials , and products in the 1 to 100  nm scale , and they have favored certain industrial segments , including foodstuffs , electronics , textiles , pharmaceuticals , biotechnological , cosmetics , medicine , agriculture , and national security [ 15 ] . one of the most well - known nanoproducts is the silver nanoparticle , which is widely used in the textile industry in brazil . water- or oil - resistant and antibacterial clothes are some examples of the application of nanotechnology in this industry . however , the attractive properties of the nanomaterials , such as small particle size , varied shape and high superficial area , may lead to their causing damage to living organisms [ 711 ] . some studies have reported that prolonged ingestion of nanosilver can induce a rare disease which causes irreversible pigmentation of the skin .   in this context the development of analytical techniques for the determination of silver in several kinds of samples has been attracting the attention of many researcher groups [ 1222 ] . solid phase extraction ( spe ) , liquid - liquid extraction , and liquid membrane extraction   are widely applied for the separation and preconcentration of metals , among a variety of other methods . in particular , the procedures based on spe offer some advantages over the others , such as less waste generation , milder matrix effects , availability and easy recovery of the solid phase , achievement of higher preconcentration factors , easy adaptation of the solid phase in a minicolumn coupled to a continuous flow preconcentration system , and , in general , no requirement for the use of toxic solvents . another important advantage of this technique is the possibility to use a relatively simple detection system , such as faas , rather than techniques without flame , which require more sophisticated equipment . flow injection ( fi ) online separation and preconcentration with minicolumns containing an appropriate adsorbent is an area that has shown great promise and , in fact , has become one of the most active research fields in automated solution analysis in recent years . this method offers some very favorable features in relation to batch systems , such as higher sample throughput , better efficiency and enrichment reproducibility , low reagent and sample consumption , lower risk of contamination , and simple automated operation . currently , many fi online preconcentration systems have been coupled with the faas technique , due to the low cost of this equipment and its high analytical velocity . there are many materials found in the environment formed by macromolecules containing several functional groups which are able to interact with metals through different phenomena such as adsorption , chemisorption , complexation , and ion exchange . the materials with these characteristics are known as biosorbents , and they can be applied in spe procedures in an fi system .   our research group has been exploring the use of new biosorbent materials for metal determination in biological and environmental matrices . among the biosorbents explored are vermicompost , moringa oleifera seeds , and , currently , the termite digestion product . termite digestion product is produced through the biochemical transformation of cellulose that occurs inside the termite intestine with the help of protozoa . this product is released in small cylindrical - shaped particles with active sites on their surface that can be deprotonated according to the ph , leaving their surface negatively charged , thus facilitating the interaction with metallic ions . due to its appropriate and monodispersive particle size distribution , the termite digestion product can be easily accommodated in an spe cartridge .   in this study , the use of termite digestion product for the determination of silver in the wash water of clothes impregnated with silver nanoparticle using an fi online preconcentration system coupled with faas is proposed . in addition , the optimization of this system was carried out using a multivariate methodology . a varian spectraa 50 ( victoria , australia ) flame atomic absorption spectrometer ( faas ) , equipped with a silver hollow cathode lamp and a deuterium lamp for background correction , was used for the detection of silver . a mettler toledo 320 ph meter was used to adjust the ph of the samples and working solutions . an ismatec - ipc ( zurich , switzerland ) peristaltic pump equipped with eight channels and tygon and polyethylene tubes was used to pump the solutions through the minicolumn ( 60  mm  3  mm ) in the elution and preconcentration steps . infrared ( ir ) spectra were recorded on an abb - bomem fourier transform - ir spectrometer , model ftla 2000 using the kbr disc method . the chn analysis was carried out on a ce instruments , model ea 1110 chns elemental analyzer . scanning electronic microscopy ( philips xl-30 microscope ) and semiquantitative microanalysis using energy dispersion spectroscopy ( eds ) were carried out to characterize the termite digestion product used as the biosorbent . ultrapure water from a milli - q ( millipore , bedford , ma , usa ) water purification system was used to prepare all solutions . the laboratory glassware was washed with a 2% ( v / v ) extran ( merck , darmstadt , germany ) solution , kept overnight in 10% ( v / v ) nitric acid solution , and then washed with deionized water . working standard solutions of silver in appropriate concentrations were prepared daily by dilution of a 1000  mg nitric acid solutions ( merck ) used as the eluent in the flow system were prepared by appropriate dilution of the concentrated acid with water . buffer solutions were prepared with appropriate amounts of ammonia ( merck ) , sodium citrate , sodium hydrogen phosphate , or tris(hydroxymethyl)aminomethane ,   the final ph being adjusted to the required value with hcl 6  mol  l or naoh 6  mol  l. the wash water samples of clothes impregnated with silver nanoparticles were submitted to the following procedure : addition of 2  ml of concentrated nitric acid to 100  ml sample , magnetically stirring the solution for 24 hours . at this stage the silver nanoparticles were oxidized to ag , and the sample was submitted to the preconcentration system . the online flow system used for the development of the proposed method is shown in figure 1 . the flow system consists of a peristaltic pump equipped with tygon tubes , four three - way solenoid valves , and a minicolumn filled with termite digestion product . the system was coupled to the faas . during the preconcentration step ( figure 1(a ) ) valve 1 is open and the other valves remain closed , the sample or working solutions are pumped through the minicolumn , and the effluent is discharged . in the elution step ( figure 1(b ) ) , valve 1 is closed and valves 2 , 3 , and 4 are open . thus , the eluent percolates through the minicolumn in the opposite direction to that of the preconcentration step . the point of zero charge was obtained preparing several 0.01  kcl  mol  l solutions ( 50  ml ) with ph between 2 to 12 with and without 10  g of biosorbent . these solutions were periodically agitated , and , after 48  h , their ph values were determined . a plot of ph versus phkcl was obtained to calculate the point of zero charge . the ph was calculated as the difference between the ph of the solutions with and without the biosorbent . the point of zero charge is the ph at which phkcl and ph are zero . in this study , ag is used to represent all of the chemical species containing silver ions . this study consisted of three steps : the first focused in the characterization of the biosorbent . in the second step , the optimum experimental conditions for the extraction of ag from aqueous solutions using the new biosorbent in an online spe system were determined through a multivariate optimization technique . in the last step , the analytical features of merit were obtained for the optimized online system using the termite digestion product as the biosorbent , and the proposed method was applied to the determination of silver from wash water samples of clothes impregnated with silver nanoparticles .   among the dry wood termites , the species that cause most damage in the world belong to the cryptotermes genus , cryptotermes brevis being the most commonly cited in brazil . the wood used in this study was pine because of its high availability , and it is the kind of wood most frequently attacked by termites . samples of the termite digestion product were collected from different sources , and their extraction capacity was tested using the analytical preconcentration methods proposed in this study . all biosorbents studied showed similar analytical response , indicating good tolerance limits in relation to the location and environment of production . the ft - ir technique was used to study the main functional groups present in the termite digestion product , and the results are given in figure 2 . the expected presence of amine groups was not observed due to the low nitrogen concentration in the sample , approximately 1% of the total mass of sorbent . on the other hand , the elemental analysis results indicated 51.79% c , 6.28% h , and 0.97% n.   considering that termites live on wood , which basically consists of cellulose , and observing the strong band assigned to o h stretching , it is suggested that these groups are responsible for electrostatic interactions between the sorbent and metals . this is verified by the existence of a point of zero charge for the termite digestion product . the surface of the proposed biosorbent has a compact morphology , without apparent porosity and with particles of regular size and a cylindrical shape . a semiquantitative analysis of the biosorbent surface was performed through eds ( figure 4 ) and the spectrum showed peaks attributed to carbon , oxygen , and calcium , the latter being the counterion of the biosorbent active sites . through the results obtained by elemental analysis and eds , it can be estimated that the termite digestion product is composed of carbon ( 52% ) , hydrogen ( 6% ) , oxygen ( 39% ) , nitrogen ( 1% ) , and calcium ( 2% ) . the result obtained in the determination of the ph value of the point of zero charge for the proposed biosorbent is shown in figure 5 . as can be observed , the same number of positive and negative charges on the surface of the termite digestion product is observed at ph 5.8 . thus , to guarantee a negatively charged surface for the proposed biosorbent , a value of ph > 5.8 was used throughout the study .   the effect of buffer type ( tris / hcl , hpo4/h2po4 , nh3/nh4 , and citrate ) on the extraction efficiency of the proposed online preconcentration system was studied using the univariate method . in this study the sample ph was changed for each buffer in order to investigate the interaction between them . according to the results shown in figure 6 , the maximum retention for silver occurred with nh3/nh4 buffer and a sample ph of 12 . in this case , there is the possibility for the formation of a positively charged silver amino complex , which can be retained by the biosorbent and would lead to higher extraction efficiency . in addition , the formation of a complex between silver ion and nh3 prevents the precipitation of ag as ag2o(s ) , explaining the better performance of ammonium buffer compared to the other buffers . a pareto chart was plotted to check the influence of the factors and their interactions in the system using the proposed biosorbent . the factors chosen considering the online preconcentration system were biosorbent mass ( 30300  mg ) , sample ph ( 812 ) , nh3/nh4 buffer concentration ( 0.010.1  mol  l ) , and sample flow rate ( 48  ml  min ) . the silver concentration used in the optimization was 50  g  l. the eluent flow rate was previously studied in the range of 48  ml  min and was not found to be a significant parameter in this system , thus being fixed at 8  ml  min . the main effects and their interactions can be observed in the pareto chart shown in figure 7 . according to this chart , the factors of sample ph and buffer concentration , and their interactions , are highly significant . the positive values obtained for buffer concentration and sample ph in this study ( + 28.06 and + 11.74 , resp . ) indicate that by increasing these factors the analytical signal will also increase . on the other hand , the negative value for sample flow rate indicates an improvement in the analytical response when the level changes from maximum to minimum . a low sample flow rate allows a better interaction between the analyte and the biosorbent surface increasing the extraction efficiency . however , the use of a low sample flow rate leads to a poor analytical frequency . thus , an intermediate flow rate ( 6  ml  min ) was considered to be most appropriate and was used without lost of analytical signal . the factorial study also demonstrated that the biosorbent mass was not a significant factor and this parameter was fixed at 300  mg . the results obtained for the full factorial study indicated that the variables buffer concentration and sample ph required a final optimization . the response surface was obtained , varying the sample ph ( 1012 ) and buffer concentration ( 0.10.5  mol  l ) , through application of the doehlert matrix and is shown in figure 8 . these results indicate that when a high buffer concentration is used the sample ph can be within the range of 10 and 12 without lost of analytical signal , showing a robust characteristic for the proposed method . a high nh3/nh4 buffer concentration leads to the formation of a positively charged silver amino complex which could favor the interaction of the silver with the sample and biosorbent surface within the optimized sample ph range . higher buffer concentration was not studied because , under these conditions , the analyte was quantitatively retained . in short , the optimized conditions for the adsorption of ag onto the termite digestion product in the proposed online system obtained using full factorial and doehlert designs are nh3/nh4 buffer at 0.50  mol  l , sample ph of 1012 , and sample flow rate of 6.0  ml  min . the performance of the preconcentration system using the termite digestion product as a biosorbent is shown in table 1 . calibration curves for ag were obtained using solutions in the concentration range of 050  g  l. the precision of the proposed method is evaluated as the relative standard deviation for a sample solution containing 15 and 35  g  l of silver , when 10.0  ml of sample was preconcentrated . the limit of detection ( lod ) is defined as the concentration of analyte that gives a response equivalent to three times the standard deviation ( sd ) of the blank ( n = 10 ) . the enrichment factor ( ef ) was calculated as the ratio of the slopes of the calibration curves obtained with and without preconcentration . the intercept and the slope of the curve with and without preconcentration , respectively , are shown below : abs =   0.0069 + 0.0069 cag and abs = 0.0079 + 0.0003 + cag . the proposed method was applied to the analysis of water used to wash pillowcases impregnated with silver nanoparticles which were obtained from a textile industry located in santa catarina , brazil . the concentration of the analyte in the wash water samples was below the detection limit of the method , as there was no spiking of the samples , and thus the metallic ions could not be detected . in order to evaluate the accuracy of the preconcentration procedure , recovery tests were carried out by adding 15  g  l of ag to all samples studied . the recovery of ag obtained ( table 2 ) from this study was between 96107% . the proposed online preconcentration system using a minicolumn filled with termite digestion product biosorbent represents an attractive alternative method to determine silver in aqueous samples . the online preconcentration procedure is simple and presented good sensitivity and precision in the determination of trace concentrations of ag in the samples analyzed by faas . the use of full factorial and doehlert designs allowed the optimization of the proposed system , while taking the interactions among the variables into consideration . this procedure was found to be more efficient than the univariate method , and its development required a low number of experiments .
a new method for ag determination in water samples using solid phase extraction ( spe ) coupled to a flow injection system and flame atomic absorption spectrometry was developed . 
 the sorbent used for ag preconcentration and extraction was the termite digestion product . 
 flow and chemical variables of the system were optimized through a multivariate procedure . 
 the factors selected were adsorbent mass , buffer type and concentration , sample ph , and sample flow rate . the detection limit and precision were 3.4  g  l1 and 3.8% ( n = 6 , 15  g  l1 ) , respectively . 
 the enrichment factor and the linear working range were , respectively , 21 and 1050  g  l1 . 
 results for recovery tests using different water samples were between 96 and 107% . 
 the proposed methodology was applied with success for the determination of ag in water used to wash clothes impregnated with silver nanoparticles , supplied by a factory located in santa catarina , brazil .