from may through october 2009 , a total of 10,624 clinical samples from 23 states throughout the united states were submitted to diatherix laboratories ( www.diatherix.com ; huntsville , al , usa ) and screened for multiple respiratory pathogen gene targets . diatherix , a reference laboratory certified by the clinical laboratory improvement amendments , provides molecular differential detection services based on target - enriched multiplex pcr technology . the respiratory infections panel detects bacterial and viral pathogens associated with respiratory infections and includes targets for the following : adenovirus ( types 3 , 4 , 7 , 21 ) , coxsackievirus , echovirus , human metapneumovirus ( types a and b ) , influenza virus ( types a and b ) , parainfluenza virus ( types 14 ) , respiratory syncytial virus ( types a and b ) , rhinovirus , acinetobacter baumannii , chlamydophila pneumoniae , haemophilus influenzae , klebsiella pneumoniae , legionella pneumophila , mycoplasma pneumoniae , neisseria meningitidis , pseudomonas aeruginosa , staphylococcus aureus , streptococcus pneumoniae , and streptococcus pyogenes ( group a ) . additionally , targets specific for pandemic ( h1n1 ) 2009 virus were developed , validated , and approved by the us food and drug administration under emergency use authorization provisions for patient testing . of the respiratory specimens shipped by overnight mail from the 23 states , > 95% were nasopharyngeal swabs in transfer buffer . high - throughput nucleic acid extraction was performed automatically by using kingfisher 96 instrumentation ( thermo scientific , hudson , nh , usa ) and magnetx chemistry ( scigenix , marietta , ga , usa ) according to manufacturers specifications . multiplex pcr amplification and luminex ( austin , tx , usa ) liquid suspension detection methods were based on internally validated protocols . reactions were amplified by using abi 9700 thermocyclers ( applied biosystems , singapore ) , and the resulting pcr products were detected by using the liquichip 200 workstation ( luminex ) according to previously described protocols ( 3,6 ) . of the 10,624 samples studied , 4,690 ( 44.1% ) were negative for all pathogens detectable with the assay . among the 7,514 ( 70.73% ) samples negative for pandemic ( h1n1 ) 2009 virus , 3 bacterial pathogens predominated : s. aureus ( 875 ; 11.65% ) , s. pneumoniae ( 573 ; 7.63% ) , and h. influenzae ( 411 ; 5.47% ) ( table 1 ) . the most common viral pathogens in the pandemic ( h1n1 ) 2009negative samples were from the family picornaviridae : coxsackie / echovirus ( 650 ; 8.65% ) , and rhinovirus ( 449 ; 5.98% ) ( table 2 ) . * screening for legionella pneumophilia detected no bacteria in any samples . s. aureus , staphylococcus aureus ; p. aeruginosa , pseudomonas aeruginosa ; s. pyogenes , streptococcus pyogenes ; n. meningitidis , neisseria meningitidis ; h. influenzae , haemophilus influenzae ; c. pneum , chlamydophila pneumoniae ; k. pneum , klebsiella pneumoniae ; m. pneum , mycoplasma pneumoniae ; s. pneum , streptococcus pneumoniae ; a. 
 baumannii , acinetobacter baumannii . * rs , respiratory syncytial . boldface indicates predominant pathogens . of the 10,624 samples studied , 3,110 ( 29.3% ) were positive for pandemic ( h1n1 ) 2009 virus , representing 52.4% of samples positive for any pathogen . among pandemic ( h1n1 ) positive samples , > 1 other pathogen was co - detected for 28% ( figure ) . the most commonly co - detected pathogens were s. aureus ( 458 ; 14.73% ) , s. pneumoniae ( 316 ; 10.16% ) , and h. influenzae ( 110 ; 3.54% ) ( table 1 ) . respiratory pathogens co - detected with pandemic ( h1n1 ) 2009 virus in clinical samples from 23 us states , may october 2009 . a significant difference ( t  =  25.6 , p = 0.01 ) was found for the age distribution between patients with positive and negative pandemic ( h1n1 ) 2009 virus results . the mean  sd age was 19.64 ( 14.45 ) years for those who were pandemic ( h1n1 ) 2009 positive and 29.67 ( 19.74 ) years for those who were negative . the median age of the 5 patients for whom 3 other pathogens were co - detected with pandemic ( h1n1 ) 2009 virus ( figure ) was 15.5 years . s. pneumoniae was detected in all 5 of these samples . for most , the other 2 pathogens were bacteria ; for only 1 , a virus ( parainfluenza ) was detected . of the 96 samples in which pandemic ( h1n1 ) 2009 virus and 2 other pathogens were co - detected ( figure ) , 30 ( 31.25% ) contained s. pneumoniae and h. influenzae . the median age of these 30 patients was 4.25 years , whereas the median age of all 96 patients was 8.2 years . the main finding of this large - scale clinical study was the co - detection of multiple pathogens with the pandemic influenza virus strain . in 44% of samples , no pathogens were detected , which may represent infection with common pathogens not detected by the assay . for example , bocavirus and all coronavirus groups not detected by the assay account for 12% and 5%10% , respectively ( 8,9 ) , of respiratory infections . an expanded test menu may improve the detection rate for such pathogens . we chose the word co - detection rather than co - infection or co - colonization because co - infection means all identified microorganisms contributed to the pathogenic effect , and co - colonization may not indicate the causative agent . the differences among the definitions have etiologic meaning , but the data presented here can not be used directly to address etiology . most samples in this study were nasal swabs rather than upper or lower respiratory tract samples . nasal swab samples have greater potential for contamination with normal flora , particularly s. aureus . however , these findings raise questions about the effectiveness of the single - agent etiology approach toward infectious diseases . pandemic ( h1n1 ) 2009 virus and multiple other pathogens are often detected during autopsy ( 1,2 ) , indicating that co - infection may play a major role in the disease process . in addition , detection of multiple pathogens is associated with increased critical illness in children ( 7 ) . the centers for disease control and prevention identified  the need for early recognition of bacterial pneumonia in persons with influenza  ( 2 ) . furthermore , the centers  underscore the importance of managing patients with influenza who also might have bacterial pneumonia with both empiric antibacterial therapy and antiviral medications current practices of clinical diagnosis based on signs and symptoms inherently lack this type of information . the true value of a multiplex molecular method of screening for infectious respiratory agents depends on the clinical relevance . among the samples with > 1 positive results , 53% had positive results for viral pathogens without co - detection of bacterial pathogens . for these patients , prescription of antimicrobial drugs on the basis of clinical findings alone could serve to spread drug resistance through selective pressure on normal flora . furthermore , limited secondary treatment resources , such as oseltamivir administration during a pandemic , could be prioritized on the basis of screening results . of the 10,624 samples studied , 70.7% were negative for the pandemic ( h1n1 ) 2009 virus strain . our findings suggest that multiplex screening for respiratory pathogens is useful for providing rapid surveillance information to inform physicians who would otherwise base decisions on clinical signs and symptoms alone . electronic reporting of empirical laboratory respiratory pathogen detection provided by a clinical laboratory improvement amendments  approved laboratory can greatly enhance surveillance data collection ( 10 ) . because most states have the authority to collect data of public health relevance ( 10 ) , the screening service provided by the diatherix laboratories could facilitate reporting of notifiable diseases .
from may through october 2009 , a total of 10,624 clinical samples from 23 us states were screened for multiple respiratory pathogen gene targets . 
 of 3,110 ( 29.3% ) samples positive for pandemic ( h1n1 ) 2009 virus , 28% contained > 1 other pathogen , most commonly staphylococcus aureus ( 14.7% ) , streptococcus pneumoniae ( 10.2% ) , and haemophilus influenzae ( 3.5% ) .