dexmedetomidine belongs to imidazole subclass of 2 adrenoceptor agonists , now widely used in anesthesia and critical care arena . it has been approved for use as sedative and analgesic in the intensive care unit ( icu ) setting . it activates presynaptic 2 adrenoceptor in the locus coeruleus of pons and inhibits the release of norepinephrine thus leading to sedation and hypnosis . the action of dexmedetomidine is unique as it mimics natural sleep , thus resulting in cooperative form of sedation , in which the patients easily transform from sleep to wakefulness when aroused . this characteristic allows for wake up test where the patients are assessed daily for improvement in neurological status , even without stopping sedation thus preventing sympathetic surges during the test . dexmedetomidine is shown to maintain cognitive and immunologic function in the sleep - deprived states as in the icu . clinical side effects of dexmedetomidine described in literature mainly focus on hemodynamic effects such as hypotension and bradycardia . most of the studies on dexmedetomidine and cerebral hemodynamics were conducted upon either animals or in healthy human volunteers with varying doses . the use of dexmedetomidine is in increasing trend particularly in patients with neurological disorders because of its property of maintaining stable hemodynamics and inducing conscious sedation . in the present study , we tested the cerebral hemodynamic effects of initial loading dose of dexmedetomidine ( 1 mcg / kg over 10 min ) with the help of transcranial color doppler ( tccd ) imaging . thirty american society of anesthesiology ( asa ) class one patients planned for lumbar discectomy procedure aged between 18 and 40 years were included after obtaining informed consent . exclusion criteria were : asa class 2 and above ; comorbidities including systemic hypertension and diabetes mellitus , preoperative heart rate < 50/min , the presence of heart block of any degree , and known allergy to dexmedetomidine or other 2 agonists . on arrival in the operating room , patients were monitored with electrocardiography ( ecg ) and pulse oximetry ( spo2 ) . under local anesthesia , intravenous and intra - arterial cannulations were performed . lactated ringer was given at rate of 4 ml / kg / hr . after application of nose clip , end tidal co2 estimation ( etco2 ) was obtained from mouthpiece and analyzed inside stream monitor ( s/5 monitor , ge healthcare , uk ) . ecg , spo2 , etco2 , respiratory rate ( rr ) , and invasive blood pressure were monitored and recorded continuously . tccd imaging using 2 mhz probe ( vivid - i , ge healthcare , uk ) was used to record cerebral blood flow velocities ( fv ) . through temporal window m1 segment ( horizontal segment ) of middle cerebral artery ( mca ) was identified according to anatomical location , depth of insonation , and direction of flow . after a steady state of fv recording , angle correction was applied so that the angle between linear segment of m1 and angle of insonation was less than 15. bilateral probes were fixed with custom made headband . fv waveforms were recorded from right and left mca for consecutive ten cardiac cycles [ figure 1 ] . middle cerebral artery flow velocity obtained from transcranial color doppler imaging loading dose of dexmedetomidine was given in the dose of 1 mcg / kg over 10 min as infusion . during infusion , blood pressure and heart rate were maintained with infusion of lactated ringer solution , injection ephedrine in 3 mg increments and injection glycopyrrolate 0.2 mg . fv waveforms were recorded from both mca after 10 min . once the study protocol was completed , infusion of dexmedetomidine was stopped and routine anesthesia was used for induction and maintenance . patients were further excluded if etco2 change of more than 2 mmhg from baseline and difficulty in locating mca with tccd . the data collected included demographic data , heart rate , invasive blood pressure , rr , spo2 , etco2 , and bilateral mca fv at baseline and at the end of the dexmedetomidine infusion . mean values of simultaneously measured invasive blood pressure recordings and the outer envelope of time averaged mca fv recordings during ten consecutive cardiac cycles were used to calculate the following cerebral hemodynamic parameters . 
 mean flow velocity ( mfv)pulsatility index ( [ pi ] = ( systolic fv  diastolic fv)/mean fv)cerebral vascular resistant index ( [ cvri ] = mean bp / mean fv)estimated cerebral perfusion pressure ( [ ecpp ] = mean fv  [ mean bp  diastolic bp / mean fv  diastolic fv ] ) andzero flow pressure ( [ zfp ] = mean bp  ecpp ) . mean flow velocity ( mfv ) pulsatility index ( [ pi ] = ( systolic fv  diastolic fv)/mean fv ) cerebral vascular resistant index ( [ cvri ] = mean bp / mean fv ) estimated cerebral perfusion pressure ( [ ecpp ] = mean fv  [ mean bp  diastolic bp / mean fv  diastolic fv ] ) and zero flow pressure ( [ zfp ] = mean bp  ecpp ) . paired t - test was used to assess the statistical difference of cerebral hemodynamic parameters ( mfv , pi , cvri , ecpp , and zfp ) between baseline and after infusion of loading dose of dexmedetomidine . a p < 0.05 was considered as statistically significant . paired t - test was used to assess the statistical difference of cerebral hemodynamic parameters ( mfv , pi , cvri , ecpp , and zfp ) between baseline and after infusion of loading dose of dexmedetomidine . a p < 0.05 was considered as statistically significant . paired t - test was used to assess the statistical difference of cerebral hemodynamic parameters ( mfv , pi , cvri , ecpp , and zfp ) between baseline and after infusion of loading dose of dexmedetomidine . a p < 0.05 was considered as statistically significant . as hemodynamic indices were assessed from both hemispheres , the study groups were subdivided and tabulated as right  and  left  representing data from mca that supplies right and left hemisphere respectively . two patients were excluded due to difficulty in localizing mca , and two patients were excluded because of change in etco2 > 2 mmhg . mean age was 32  2.7 years , mean weight was 67  7.2 kg ; 12 were male and 14 were female . vital parameters that can affect cerebral arterial fv were maintained comparable to baseline values [ table 1 ] . mfv was significantly decreased in both hemispheres after administration of loading dose of dexmedetomidine ( p < 0.001 ) . pi and cvri showed significant increase ( p < 0.05 ) after dexmedetomidine loading dose [ table 2 ] . in both hemispheres , significant decrease in ecpp ( p - 0.005 ; p < 0.001 ) and significant increase in zfp ( p < 0.001 ; p = 0.004 ) were observed [ table 2 ] . the results of our study showed that the loading dose of dexmedetomidine ( 1 mcg / kg over 10 min ) resulted in significant decrease in mfv and ecpp . we also observed an increase in pi , cvri , and zfp after loading dose . cerebral vasculature and thus cerebral blood flow is affected by various physiological factors including paco2 , pao2 , temperature , cerebral metabolic rate , and blood viscosity . these factors are often deranged by disease process or manipulated for therapeutic purposes in anesthesia and critical care environments . apart from these physiological factors , anesthetic drugs and other vasoactive drugs used in critical care environments can also directly or indirectly affect cerebral blood flow . as the use of dexmedetomidine is in increasing trend , knowledge about the cerebral vascular effects will help in the optimal use of this drug with minimal alteration in cerebral hemodynamics . transcranial doppler ( tcd ) is a simple noninvasive bedside tool to assess the cerebral blood flow pattern . the new development of transcranial color doppler with angle correction option helps to improve the sensitivity of test results . the relationship between tcd - derived fv and actual cerebral blood flow is confounded by various factors such as metabolic state , cerebral vascular resistance ( cvr ) , and cerebral perfusion pressure ( cpp ) . in a study by zornow et al . this study showed that hypnotic dose of dexmedetomidine results in a modest decrease in fv corresponding to the increase in dexmedetomidine dosage . similar results were obtained in our study where the loading dose of dexmedetomidine resulted in a decrease in blood fv . although mfv derived from tcd is not synonymous with actual cerebral blood flow , studies have shown that the changes in mfv correlate well with trends of blood flow alteration . as an experimental condition , the blood pressure and paco2 were maintained comparable to baseline during the administration of dexmedetomidine , and we have observed decrease in blood fv . however , in patients admitted in critical care units , the baseline blood pressure may be low because of the primary disease or as a side - effect of dexmedetomidine itself . administration of dexmedetomidine in this scenario can exaggerate the decrease in mfv and thus compromise on cerebral blood flow . studies have shown that gosling pi can be the reliable monitor of cerebral hemodynamic asymmetry and cpp . stimulation with 2 agents such as clonidine and dexmedetomidine has shown to cause vasoconstriction in isolated cerebral vessels and in various animal models . vasoconstrictive property and increase in cvr were also demonstrated in studies where the administration of dexmedetomidine prevented inhalational anesthetics and co2 induced vasodilatation . in a study by schregel et al . the pi showed a shortterm increase after alfentanil , decrease after halothane , and strong increase after infusion of propofol . the changes in pi values were consistent with vasodilatory effect of halothane and vasoconstrictory effect of propofol and alfentanil . increase in pi values obtained from our study suggests the vasoconstrictory effect of dexmedetomidine and possibility of decreased cerebral perfusion . this fact is also supported by the significant decrease in mfv and increase in cvri observed in our study . cpp is the net pressure gradient causing blood flow to the brain , and it is calculated as the difference between effective upstream pressure and downstream pressure . it must be maintained within narrow limits because decrease in cpp could cause brain tissue to become ischemic and an increase in cpp can lead to an increase in intracranial pressure ( icp ) , both detrimental for the survival of neurons . as the cranium is a closed cavity , the effective downstream pressure can be central venous pressure ( cvp ) or icp or cvr . thus , a more general concept of zfp has been introduced , where zfp is defined as the pressure at which flow in a vessel would cease . thus , the cpp can be estimated from the difference between map and zfp [ cpp = map  zfp ] . in our study , it is shown that , in conditions of normal icp and cvp , vascular tone may be the dominant component of zfp . increase in cvri values in our study also supports the fact that dexmedetomidine increases cerebral arteriolar tone . thus , it can be explained that the decrease in ecpp and increase in zfp observed in our study can be due to dexmedetomidine - induced cerebral vasoconstriction and increase in cvr . hypotension  is proven cardiovascular side effect of dexmedetomidine and in our study the  increase in zfp  is also demonstrated . as the cpp = map  zfp , there is possibility that administration of loading dose of dexmedetomidine can lead to reduction in cerebral blood flow . apart from cvr , intracranial pathological conditions like cerebral edema , space occupying lesions , blood clots , and elevated venous pressure have shown to increase distal resistance to blood flow . when dexmedetomidine is used in these conditions because of vasoconstrictory action , there is a possibility of exaggerated reduction in cerebral blood flow and can be detrimental to neurological outcome . results from our study show that after loading dose of dexmedetomidine , there is a possibility of decreased cpp and increased cvr . our study was done in patients without any systemic comorbid illness and in controlled condition such as maintained blood pressure , maintained etco2 , and without any other sedative drugs . in icu setting , these factors may not be under ideal conditions and presence of primary brain pathology may complicate the scenario , further compromising cerebral blood supply . there are few limitations in our study that are needed to be discussed . in this study , the initial bolus dose of dexmedetomidine ( 1 mcg / kg over 10 min ) was used . many studies have suggested that the systemic hemodynamic effects such as hypotension and bradycardia can be minimized by omitting the bolus dose and initiating the maintenance dose as an infusion . like the systemic effects thus , further studies are warranted to assess the effect of dexmedetomidine on cerebral hemodynamics with varying maintenance doses . decrease in fv due to metabolic suppression could not be ruled out in our study . studies have shown altered cerebral blood flow and abnormal vascular reactivity in patients with intracranial pathological conditions like infection , trauma , tumor , and vascular malformations . further adequately powered studies are warranted to address the effect of cerebral hemodynamic effect of dexmedetomidine in pathological conditions involving the brain . , the use of monitors such as laser doppler flowmetry and microdialysis can further help to understand the action of dexmedetomidine at the tissue level . the relationship between tcd - derived fv and actual cerebral blood flow is confounded by various factors such as metabolic state , cerebral vascular resistance ( cvr ) , and cerebral perfusion pressure ( cpp ) . in a study by zornow et al . , six healthy volunteers were infused with four different doses of dexmedetomidine . this study showed that hypnotic dose of dexmedetomidine results in a modest decrease in fv corresponding to the increase in dexmedetomidine dosage . similar results were obtained in our study where the loading dose of dexmedetomidine resulted in a decrease in blood fv . although mfv derived from tcd is not synonymous with actual cerebral blood flow , studies have shown that the changes in mfv correlate well with trends of blood flow alteration . as an experimental condition , the blood pressure and paco2 were maintained comparable to baseline during the administration of dexmedetomidine , and we have observed decrease in blood fv . however , in patients admitted in critical care units , the baseline blood pressure may be low because of the primary disease or as a side - effect of dexmedetomidine itself . administration of dexmedetomidine in this scenario can exaggerate the decrease in mfv and thus compromise on cerebral blood flow . studies have shown that gosling pi can be the reliable monitor of cerebral hemodynamic asymmetry and cpp . stimulation with 2 agents such as clonidine and dexmedetomidine has shown to cause vasoconstriction in isolated cerebral vessels and in various animal models . vasoconstrictive property and increase in cvr were also demonstrated in studies where the administration of dexmedetomidine prevented inhalational anesthetics and co2 induced vasodilatation . in a study by schregel et al . the pi showed a shortterm increase after alfentanil , decrease after halothane , and strong increase after infusion of propofol . the changes in pi values were consistent with vasodilatory effect of halothane and vasoconstrictory effect of propofol and alfentanil . increase in pi values obtained from our study suggests the vasoconstrictory effect of dexmedetomidine and possibility of decreased cerebral perfusion . this fact is also supported by the significant decrease in mfv and increase in cvri observed in our study . cpp is the net pressure gradient causing blood flow to the brain , and it is calculated as the difference between effective upstream pressure and downstream pressure . it must be maintained within narrow limits because decrease in cpp could cause brain tissue to become ischemic and an increase in cpp can lead to an increase in intracranial pressure ( icp ) , both detrimental for the survival of neurons . as the cranium is a closed cavity , the effective downstream pressure can be central venous pressure ( cvp ) or icp or cvr . thus , a more general concept of zfp has been introduced , where zfp is defined as the pressure at which flow in a vessel would cease . thus , the cpp can be estimated from the difference between map and zfp [ cpp = map  zfp ] . in our study , it is shown that , in conditions of normal icp and cvp , vascular tone may be the dominant component of zfp . increase in cvri values in our study also supports the fact that dexmedetomidine increases cerebral arteriolar tone . thus , it can be explained that the decrease in ecpp and increase in zfp observed in our study can be due to dexmedetomidine - induced cerebral vasoconstriction and increase in cvr . hypotension  is proven cardiovascular side effect of dexmedetomidine and in our study the  increase in zfp  is also demonstrated . as the cpp = map  zfp , there is possibility that administration of loading dose of dexmedetomidine can lead to reduction in cerebral blood flow . apart from cvr , intracranial pathological conditions like cerebral edema , space occupying lesions , blood clots , and elevated venous pressure have shown to increase distal resistance to blood flow . when dexmedetomidine is used in these conditions because of vasoconstrictory action , there is a possibility of exaggerated reduction in cerebral blood flow and can be detrimental to neurological outcome . results from our study show that after loading dose of dexmedetomidine , there is a possibility of decreased cpp and increased cvr . our study was done in patients without any systemic comorbid illness and in controlled condition such as maintained blood pressure , maintained etco2 , and without any other sedative drugs . in icu setting , these factors may not be under ideal conditions and presence of primary brain pathology may complicate the scenario , further compromising cerebral blood supply . there are few limitations in our study that are needed to be discussed . in this study , the initial bolus dose of dexmedetomidine ( 1 mcg / kg over 10 min ) was used . many studies have suggested that the systemic hemodynamic effects such as hypotension and bradycardia can be minimized by omitting the bolus dose and initiating the maintenance dose as an infusion . like the systemic effects thus , further studies are warranted to assess the effect of dexmedetomidine on cerebral hemodynamics with varying maintenance doses . decrease in fv due to metabolic suppression could not be ruled out in our study . studies have shown altered cerebral blood flow and abnormal vascular reactivity in patients with intracranial pathological conditions like infection , trauma , tumor , and vascular malformations . further adequately powered studies are warranted to address the effect of cerebral hemodynamic effect of dexmedetomidine in pathological conditions involving the brain . however , the use of monitors such as laser doppler flowmetry and microdialysis can further help to understand the action of dexmedetomidine at the tissue level . the relationship between tcd - derived fv and actual cerebral blood flow is confounded by various factors such as metabolic state , cerebral vascular resistance ( cvr ) , and cerebral perfusion pressure ( cpp ) . in a study by zornow et al . , six healthy volunteers were infused with four different doses of dexmedetomidine . this study showed that hypnotic dose of dexmedetomidine results in a modest decrease in fv corresponding to the increase in dexmedetomidine dosage . similar results were obtained in our study where the loading dose of dexmedetomidine resulted in a decrease in blood fv . although mfv derived from tcd is not synonymous with actual cerebral blood flow , studies have shown that the changes in mfv correlate well with trends of blood flow alteration . as an experimental condition , the blood pressure and paco2 were maintained comparable to baseline during the administration of dexmedetomidine , and we have observed decrease in blood fv . however , in patients admitted in critical care units , the baseline blood pressure may be low because of the primary disease or as a side - effect of dexmedetomidine itself . administration of dexmedetomidine in this scenario can exaggerate the decrease in mfv and thus compromise on cerebral blood flow . studies have shown that gosling pi can be the reliable monitor of cerebral hemodynamic asymmetry and cpp . stimulation with 2 agents such as clonidine and dexmedetomidine has shown to cause vasoconstriction in isolated cerebral vessels and in various animal models . vasoconstrictive property and increase in cvr were also demonstrated in studies where the administration of dexmedetomidine prevented inhalational anesthetics and co2 induced vasodilatation . in a study by schregel et al . the pi showed a shortterm increase after alfentanil , decrease after halothane , and strong increase after infusion of propofol . the changes in pi values were consistent with vasodilatory effect of halothane and vasoconstrictory effect of propofol and alfentanil . increase in pi values obtained from our study suggests the vasoconstrictory effect of dexmedetomidine and possibility of decreased cerebral perfusion . this fact is also supported by the significant decrease in mfv and increase in cvri observed in our study . cpp is the net pressure gradient causing blood flow to the brain , and it is calculated as the difference between effective upstream pressure and downstream pressure . it must be maintained within narrow limits because decrease in cpp could cause brain tissue to become ischemic and an increase in cpp can lead to an increase in intracranial pressure ( icp ) , both detrimental for the survival of neurons . as the cranium is a closed cavity , the effective downstream pressure can be central venous pressure ( cvp ) or icp or cvr . thus , a more general concept of zfp has been introduced , where zfp is defined as the pressure at which flow in a vessel would cease . thus , the cpp can be estimated from the difference between map and zfp [ cpp = map  zfp ] . in our study , it is shown that , in conditions of normal icp and cvp , vascular tone may be the dominant component of zfp . increase in cvri values in our study also supports the fact that dexmedetomidine increases cerebral arteriolar tone . thus , it can be explained that the decrease in ecpp and increase in zfp observed in our study can be due to dexmedetomidine - induced cerebral vasoconstriction and increase in cvr . hypotension  is proven cardiovascular side effect of dexmedetomidine and in our study the  increase in zfp  is also demonstrated . as the cpp = map  zfp , there is possibility that administration of loading dose of dexmedetomidine can lead to reduction in cerebral blood flow . apart from cvr , intracranial pathological conditions like cerebral edema , space occupying lesions , blood clots , and elevated venous pressure have shown to increase distal resistance to blood flow . when dexmedetomidine is used in these conditions because of vasoconstrictory action , there is a possibility of exaggerated reduction in cerebral blood flow and can be detrimental to neurological outcome . results from our study show that after loading dose of dexmedetomidine , there is a possibility of decreased cpp and increased cvr . our study was done in patients without any systemic comorbid illness and in controlled condition such as maintained blood pressure , maintained etco2 , and without any other sedative drugs . in icu setting , these factors may not be under ideal conditions and presence of primary brain pathology may complicate the scenario , further compromising cerebral blood supply . there are few limitations in our study that are needed to be discussed . in this study , the initial bolus dose of dexmedetomidine ( 1 mcg / kg over 10 min ) was used . many studies have suggested that the systemic hemodynamic effects such as hypotension and bradycardia can be minimized by omitting the bolus dose and initiating the maintenance dose as an infusion . like the systemic effects thus , further studies are warranted to assess the effect of dexmedetomidine on cerebral hemodynamics with varying maintenance doses . decrease in fv due to metabolic suppression could not be ruled out in our study . studies have shown altered cerebral blood flow and abnormal vascular reactivity in patients with intracranial pathological conditions like infection , trauma , tumor , and vascular malformations . further adequately powered studies are warranted to address the effect of cerebral hemodynamic effect of dexmedetomidine in pathological conditions involving the brain . , the use of monitors such as laser doppler flowmetry and microdialysis can further help to understand the action of dexmedetomidine at the tissue level . in summary , we observed that loading dose of dexmedetomidine ( 1 mcg / kg over 10 min ) resulted in decrease in fv and estimated cpp even after maintaining blood pressure comparable to baseline values . all these findings collectively suggest the possibility of a decrease in cerebral blood flow after loading dose of dexmedetomidine ( 1 mcg / kg over 10 min ) . further studies are needed to evaluate the effect of dexmedetomidine on various other pathological conditions involving brain like traumatic brain injury and vascular malformations .
background : dexmedetomidine has been widely used in critical care settings because of its property of maintaining stable hemodynamics and inducing conscious sedation . 
 the use of dexmedetomidine is in increasing trend particularly in patients with neurological disorders . 
 very few studies have focused on the cerebral hemodynamic effects of dexmedetomidine . 
 this study is aimed to address this issue.methods:thirty patients without any intracranial pathology were included in this study . middle cerebral artery flow velocity obtained from transcranial color doppler 
 was used to assess the cerebral hemodynamic indices . 
 mean flow velocity ( mfv ) , pulsatility index ( pi ) , cerebral vascular resistant index ( cvri ) , estimated cerebral perfusion pressure ( ecpp ) , and zero flow pressure ( zfp ) were calculated bilaterally at baseline and after infusion of injection dexmedetomidine 1 mcg / kg over 10 min.results:twenty-six patients completed the study protocol . 
 after administration of loading dose of dexmedetomidine , mfv and ecpp values were significantly decreased in both hemispheres ( p < 0.05 ) ; pi , cvri , and zfp values showed significant increase ( p < 0.05 ) after dexmedetomidine infusion.conclusion:increase in pi , cvri , and zfp suggests that there is a possibility of an increase in distal cerebral vascular resistance ( cvr ) with loading dose of dexmedetomidine . 
 decrease in mfv and ecpp along with an increase in cvr may lead to a decrease in cerebral perfusion . 
 this effect can be exaggerated in patients with preexisting neurological illness . 
 further studies are needed to evaluate the effect of dexmedetomidine on various other pathological conditions involving brain like traumatic brain injury and vascular malformations .