persicaria minor plants were grown on an ms solid medium in a growth chamber at 25    2  c with a photoperiod of 16  h of light and 8  h of darkness . healthy , 45  day plants were selected for mj and aba elicitation , and leaves were sprayed with 100  m of mj and 100  m of aba , while the control plants were sprayed with distilled water . for mj - treatment , leaves sample were harvested after 2  days ( maximum gene expression was previosly reported after 2  days ) , whereas ; samples for aba - treatment were harvested after 3  days . the harvested samples were blotted dry , frozen in liquid nitrogen and stored at   80  c for further total rna extraction . total rna from leaf samples were extracted using plant rna purification reagent ( invitrogen , usa ) based on manufacturer 's protocol . quantitation of extracted total rna was carried out using nanodrop 1000 ( thermo fisher scientific inc . , usa ) whereas , the integrity of was determined by agilent 2100 bioanalyzer ( agilent technology , usa ) , respectively . total rnas with a rin value nebnext multiplex small rna library prep set for illumina ( set 1 ) kit was used for library construction using the manufacturer 's protocol . the small - rna samples were sequenced using the illumina hiseq 2500 platform . reads with 50  bp ( single - end read ) were generated for individual samples . the remaining sequences were filtered and the sequences between 18  bp to 30  bp were obtained ( table 1 ) . firstly , the sequences were mapped against 73 plant species data from mirbase version 21 . the sequences with no hit in the mirbase were used for further annotation against rfam database ( table 2 ) for non - coding rnas ( trna , snorna , snrna , and rrna ) , . the remaining sequences having no hit with the above databases were categorized as unannotated.table 1raw reads pre-analysis.table 1sra idtotal number of readsnumber of reads after trimmingdiscarded readssrx2655642 ( mj - treated)33,496,29219,094,70614,401,586srx2655643 ( mj - treated)47,378,86023,823,12723,555,733srx2655644 ( aba - treated)3,940,9412,459,7791,481,162srx2655645 ( aba - treated)30,566,19120,683,7629,882,429srx2655646 ( control)11,223,1896,609,3154,613,874srx2655647 ( control)26,046,62015,337,04610,709,574table 2raw reads annotation analysis.table 2sra idtotal number of sequence tagsannotation by mirbaseannotation by rfamunannotatedsrx2655642 ( mj - treated)1,923,2352899110,8341,809,502srx2655643 ( mj - treated)2,403,18826461268,6912,271,792srx2655644 ( aba - treated)496,30623136,572459,503srx2655645 ( aba - treated)2,665,163845101,1412,563,177srx2655646 ( control)1,194,072154874,2741,118,250srx2655647 ( control)2,511,22170093,9752,416,546 raw reads pre - analysis . the expression of mirnas in treated and control libraries was compared . to proceed with the differential expression analysis , the first step in the statistical analysis was the normalization of the data to transcripts per million ( tpm ) which was done using criterion : tpm  =  ( actual mirna count / total count of clean reads )    1,000,000 . after the data normalization , the fold change ( fold change  =  log2 ( mirna tpm in the treatment library / mirna tpm in the control library ) and p - value was calculated using baggerly 's test . finally , differentially expressed mirnas were filtered using false discovery rates ( fdr )    0.05 and the absolute value log2 ratio    1 . table 3 showed the number of mirnas that significantly affected by both treatment.table 3differential mirnas expression analysis.table 3.treatmentstotal mirnas significantly affectednumber of mirnas upregulatednumber of mirnas downregulatedmj treatment30127328aba treatment33429 differential mirnas expression analysis . persicaria minor plants were grown on an ms solid medium in a growth chamber at 25    2  c with a photoperiod of 16  h of light and 8  h of darkness . healthy , 45  day plants were selected for mj and aba elicitation , and leaves were sprayed with 100  m of mj and 100  m of aba , while the control plants were sprayed with distilled water . for mj - treatment , leaves sample were harvested after 2  days ( maximum gene expression was previosly reported after 2  days ) , whereas ; samples for aba - treatment were harvested after 3  days . the harvested samples were blotted dry , frozen in liquid nitrogen and stored at   80  c for further total rna extraction . total rna from leaf samples were extracted using plant rna purification reagent ( invitrogen , usa ) based on manufacturer 's protocol . quantitation of extracted total rna was carried out using nanodrop 1000 ( thermo fisher scientific inc . , usa ) whereas , the integrity of was determined by agilent 2100 bioanalyzer ( agilent technology , usa ) , respectively . total rnas with a rin value nebnext multiplex small rna library prep set for illumina ( set 1 ) kit was used for library construction using the manufacturer 's protocol . the small - rna samples were sequenced using the illumina hiseq 2500 platform . reads with 50  bp ( single - end read ) were generated for individual samples . the remaining sequences were filtered and the sequences between 18  bp to 30  bp were obtained ( table 1 ) . firstly , the sequences were mapped against 73 plant species data from mirbase version 21 . the sequences with no hit in the mirbase were used for further annotation against rfam database ( table 2 ) for non - coding rnas ( trna , snorna , snrna , and rrna ) , . the remaining sequences having no hit with the above databases were categorized as unannotated.table 1raw reads pre-analysis.table 1sra idtotal number of readsnumber of reads after trimmingdiscarded readssrx2655642 ( mj - treated)33,496,29219,094,70614,401,586srx2655643 ( mj - treated)47,378,86023,823,12723,555,733srx2655644 ( aba - treated)3,940,9412,459,7791,481,162srx2655645 ( aba - treated)30,566,19120,683,7629,882,429srx2655646 ( control)11,223,1896,609,3154,613,874srx2655647 ( control)26,046,62015,337,04610,709,574table 2raw reads annotation analysis.table 2sra idtotal number of sequence tagsannotation by mirbaseannotation by rfamunannotatedsrx2655642 ( mj - treated)1,923,2352899110,8341,809,502srx2655643 ( mj - treated)2,403,18826461268,6912,271,792srx2655644 ( aba - treated)496,30623136,572459,503srx2655645 ( aba - treated)2,665,163845101,1412,563,177srx2655646 ( control)1,194,072154874,2741,118,250srx2655647 ( control)2,511,22170093,9752,416,546 raw reads pre - analysis . the expression of mirnas in treated and control libraries was compared . to proceed with the differential expression analysis , the first step in the statistical analysis was the normalization of the data to transcripts per million ( tpm ) which was done using criterion : tpm  =  ( actual mirna count / total count of clean reads )    1,000,000 . after the data normalization , the fold change ( fold change  =  log2 ( mirna tpm in the treatment library / mirna tpm in the control library ) and p - value was calculated using baggerly 's test . finally , differentially expressed mirnas were filtered using false discovery rates ( fdr )    0.05 and the absolute value log2 ratio    1 . table 3 showed the number of mirnas that significantly affected by both treatment.table 3differential mirnas expression analysis.table 3.treatmentstotal mirnas significantly affectednumber of mirnas upregulatednumber of mirnas downregulatedmj treatment30127328aba treatment33429 differential mirnas expression analysis .
persicaria minor ( kesum ) is an important medicinal plant with high level of secondary metabolite contents , especially , terpenoids and flavonoids . 
 previous studies have revealed that application of exogenous phytohormone could increase secondary metabolite contents of the plant . 
 micrornas ( mirnas ) are small rnas that play important regulatory roles in various biological processes . in order to explore the possible role of mirna in the regulation of these phytohormones signaling pathway and uncovering their potential correlation , 
 we , for the first time , have generated the smallrna library of kesum plant . 
 the library was developed in response to methyl jasmonate ( mj ) and abscisic acid ( aba ) treatment by using next - generation sequencing technology . 
 raw reads have been deposited to sra database with the accession numbers , srx2655642 and srx2655643 ( mj - treated ) , srxsrx2655644 and srx2655645 ( aba - treated ) and srx2655646and srx2655647 ( control ) .