laparostomy or open abdomen has developed over the last 20  years as a lifesaving intervention for surgical emergencies such as abdominal compartment syndrome , wound dehiscence , trauma , and intra - abdominal sepsis [ 15 ] . primary goals of wound management include avoidance of mechanical contamination of abdominal viscera , active removal of exudates , third space fluid loss estimation , infection control , and prevention of intestinal fistulae . various materials have been used to dress the open abdomen including intravenous fluid bags , goretex , bogota bags , and sandwiched gauze dressings . negative pressure wound therapy ( npwt ) applies the principle of topical negative pressure ( tnp ) through the use of suction tubing and an occlusive dressing ( the vacuum pack method ) to divert contaminated effluent away from the peritoneal cavity . temporary closure of the abdominal cavity with plastic bags , silicone sheets , absorbable , and non - absorbable meshes sutured to the fascial or skin edges do not facilitate definitive closure of the abdominal wall . skin - only closure or split - thickness skin grafting may be used for covering the bowels and omentum [ 1 , 7 , 8 ] . the major drawback with these techniques is the formation of extensive ventral hernias that have to be dealt with later . the use of airtight dressings and vacuum therapy for handling the open abdomen has improved care and increased the possibility for closure of the open abdomen . however , the method has occasionally been associated with increased development of intestinal fistulae and enteroatmospheric fistulae [ 913 ] . the mechanical effects of npwt may result in ischemia , which in turn may promote the development of fistulae . there have been several reports over the years showing excellent clinical results of npwt [ 6 , 9 , 11 , 1418 ] . however , in november 2009 , the food and drug administration ( fda ) , usa , issued a preliminary warning regarding incoming reports of rare but serious complications associated with the use of npwt . the warning was issued after the fda received reports of six deaths and 77 injuries associated with npwt systems over the past 2  years . bleeding was the most serious complication and was reported in 6 death and 17 injury cases . twenty - seven reports indicated infection from original open infected wounds or from retention of dressing pieces in the wound . retention of foam dressing pieces and foam adhering to tissues or imbedded in the wound were noted in 32 injury reports . the majority of these patients required surgical procedures for removal of the retained pieces , wound debridement , and treatment of wound dehiscence , as well as additional hospitalization and antibiotic therapy . in the present study , we examine whether npwt applied in the open abdomen induces any changes in the microvascular blood flow in the intestines or in the omentum . theoretically , ischemia and secondary necrosis in the intestinal wall and the omentum could promote the development of intestinal fistulae and enteroatmospheric fistulae . blood flow changes were studied in the omentum and in the intestinal wall during exposure to tpn between 50 and 170  mmhg . to our knowledge , no such study has previously been conducted . six domestic pigs of both genders with a median weight of 60  kg were used . the investigation complied with the  guide for the care and use of laboratory animals  as recommended by the u.s . all the animals were pre - medicated intramuscularly with ketamine ( 30  mg / kg ) before being brought into the laboratory . before commencing surgery , sodium thiopental ( 5  mg / kg ) , atropine ( 0.02  mg / kg ) , and pancuronium ( 0.5  mg / kg ) were given intravenously . intubation was performed with a portex endotracheal tube ( 7.5  mm internal diameter , medcompare , south san francisco , ca ) . a servo - ventilator ( siemens elema 300a , stockholm , sweden ) was used for mechanical ventilation throughout the experiments . the ventilator settings used were : minute volume  =  100  ml / kg , fio2  =  0.5 , breathing frequency  =  16 breaths / minute , and positive end expiratory pressure  =  5  cm  h2o . mg / kg / hour propofol ( diprivan , astrazeneca , sweden ) , 0.15  mg / kg / hour fentanyl ( leptanal , lilly , france ) , and 0.6  mg / kg / hour pancuronium ( pavulon , organon teknika , boxtel , the netherlands ) . the v.a.c. granu foam abdominal dressing system ( kci , san antonio , tx ) was used . the visceral protective layer was cut to an appropriate size , extending into the paracolic gutters on both sides ( about 35  cm wide and 35  cm long ) . a layer of polyurethane granu foam was placed on top of the visceral protective layer between the edges of the wound . the wound was covered with self - adhesive polyethylene drape , a track pad was inserted through the drape ( all from v.a.c . , kci , san antonio , tx ) , and then connected to a continuous vacuum source . microvascular blood flow was measured by ldv ( transonic laser doppler monitor , blf21 , maastricht , the netherlands ) using a technique that quantifies the sum of the motion of the red blood cells in a specific volume . this method is applied extensively in plastic surgery procedures and employs a fiberoptic probe carrying a beam of light . light impinging on cells in motion undergoes a change in wavelength ( doppler shift ) while light impinging on static objects remains unchanged . the magnitude and frequency distributions of the changes are directly related to the number and velocity of red blood cells . the information is collected by a returning fiber , converted into an electronic signal and analyzed . laser doppler probes were inserted into the omentum and also into the intestinal wall of the ileac loop , which was sutured to the inner surface center of the visceral protective layer . 1the figure shows a schematic drawing of the negative wound pressure therapy of the abdomen ( vacuum pack method ) setup and the location of the laser doppler probes . the part of the intestinal wall and the part of the omentum where the blood flow was measured was sutured to the inner surface of the visceral protective layer . 2photograph of the experimental setup , with the laser doppler machine , and the laser doppler filament probes used to measure blood flow of the small intestinal wall the figure shows a schematic drawing of the negative wound pressure therapy of the abdomen ( vacuum pack method ) setup and the location of the laser doppler probes . the part of the intestinal wall and the part of the omentum where the blood flow was measured was sutured to the inner surface of the visceral protective layer . all probe locations were controlled after every experiment photograph of the experimental setup , with the laser doppler machine , and the laser doppler filament probes used to measure blood flow of the small intestinal wall the microvascular blood flow was measured continuously by the laser doppler filament probes . after the setup , a restoration time of 30  min was allowed , where no negative pressure was applied to the npwt dressing . recordings were made before npwt ( baseline  =  0  mmhg ) and during exposure to npwt at 50 70 , 100 , 120 , 150 , and 170  mmhg . calculations and statistical analysis were performed using graphpad 5.0 software ( san diego , ca ) . the mann wallis test with dunn 's test for multiple comparisons when comparing three groups or more . significance was defined as p  <  0.05 ( * ) , p  <  0.01 ( * * ) , p  <  0.001 ( * * * ) , and p  >  0.05 ( not significant , n.s . ) . the values given are the mean of ten measurements and the standard error on the mean ( sem ) . six domestic pigs of both genders with a median weight of 60  kg were used . the investigation complied with the  guide for the care and use of laboratory animals  as recommended by the u.s . all the animals were pre - medicated intramuscularly with ketamine ( 30  mg / kg ) before being brought into the laboratory . before commencing surgery , sodium thiopental ( 5  mg / kg ) , atropine ( 0.02  mg / kg ) , and pancuronium ( 0.5  mg / kg ) were given intravenously . intubation was performed with a portex endotracheal tube ( 7.5  mm internal diameter , medcompare , south san francisco , ca ) . a servo - ventilator ( siemens elema 300a , stockholm , sweden ) was used for mechanical ventilation throughout the experiments . the ventilator settings used were : minute volume  =  100  ml / kg , fio2 =  0.5 , breathing frequency  =  16 breaths / minute , and positive end expiratory pressure  =  5  cm  h2o . mg / kg / hour propofol ( diprivan , astrazeneca , sweden ) , 0.15  mg / kg / hour fentanyl ( leptanal , lilly , france ) , and 0.6  mg / kg / hour pancuronium ( pavulon , organon teknika , boxtel , the netherlands ) . the v.a.c. granu foam abdominal dressing system ( kci , san antonio , tx ) was used . the visceral protective layer was cut to an appropriate size , extending into the paracolic gutters on both sides ( about 35  cm wide and 35  cm long ) . a layer of polyurethane granu foam was placed on top of the visceral protective layer between the edges of the wound . the wound was covered with self - adhesive polyethylene drape , a track pad was inserted through the drape ( all from v.a.c . , kci , san antonio , tx ) , and then connected to a continuous vacuum source . microvascular blood flow was measured by ldv ( transonic laser doppler monitor , blf21 , maastricht , the netherlands ) using a technique that quantifies the sum of the motion of the red blood cells in a specific volume . this method is applied extensively in plastic surgery procedures and employs a fiberoptic probe carrying a beam of light . light impinging on cells in motion undergoes a change in wavelength ( doppler shift ) while light impinging on static objects remains unchanged . the magnitude and frequency distributions of the changes are directly related to the number and velocity of red blood cells . the information is collected by a returning fiber , converted into an electronic signal and analyzed . laser doppler probes were inserted into the omentum and also into the intestinal wall of the ileac loop , which was sutured to the inner surface center of the visceral protective layer . the probe location was confirmed following completion of the experiments ( fig .  1 ) . 1the figure shows a schematic drawing of the negative wound pressure therapy of the abdomen ( vacuum pack method ) setup and the location of the laser doppler probes . the part of the intestinal wall and the part of the omentum where the blood flow was measured was sutured to the inner surface of the visceral protective layer . 2photograph of the experimental setup , with the laser doppler machine , and the laser doppler filament probes used to measure blood flow of the small intestinal wall the figure shows a schematic drawing of the negative wound pressure therapy of the abdomen ( vacuum pack method ) setup and the location of the laser doppler probes . the part of the intestinal wall and the part of the omentum where the blood flow was measured was sutured to the inner surface of the visceral protective layer . all probe locations were controlled after every experiment photograph of the experimental setup , with the laser doppler machine , and the laser doppler filament probes used to measure blood flow of the small intestinal wall the microvascular blood flow was measured continuously by the laser doppler filament probes . after the setup , a restoration time of 30  min was allowed , where no negative pressure was applied to the npwt dressing . recordings were made before npwt ( baseline  =  0  mmhg ) and during exposure to npwt at 50 70 , 100 , 120 , 150 , and 170  mmhg . laser doppler velocimetry measurements were performed on six pigs . the output was recorded continuously using the transonic laser doppler monitor . calculations and statistical analysis were performed using graphpad 5.0 software ( san diego , ca ) . the mann wallis test with dunn 's test for multiple comparisons when comparing three groups or more . significance was defined as p  <  0.05 ( * ) , p  <  0.01 ( * * ) , p  <  0.001 ( * * * ) , and p  >  0.05 ( not significant , n.s . ) . the values given are the mean of ten measurements and the standard error on the mean ( sem ) . the microvascular blood flow was measured in the small intestinal loop of the abdomen , sutured to the dressing . the blood flow was 2.7 ( 0.2 ) pu before and 2.0 ( 0.2 ) pu ( * p  <  0.05 ) after application of 50  mmhg , and 3.8 ( 0.4 ) pu before and 2,5 ( 0.2 ) pu ( * p  <  0.05 ) after application of 70  mmhg , and 4.0 ( 0.6 ) pu before and 2.7 ( 0.4 ) pu ( * * p  <  0.01 ) , after application of 100  mmhg , and 3.8 pu ( 0.6 ) before and 2.3 ( 0.3 ) pu ( * * p  < 0.01 ) after application of 120  mmhg , and 3.5 ( 0.6 ) pu before and 1.9 ( 0.3 ) pu ( * * p  < 0.01 ) after application of 150  mmhg , and 3.6 ( 0.6 ) pu before and 1.5 ( 0.2 ) pu ( * * p  < 0.01 ) after application of 170  mmhg.fig . 3microvascular blood flow measured using laser doppler velocimetry in intestinal wall exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d 120  mmhg , e 150  mmhg , f 170  mmhg . the measurements were performed in an intestinal loop , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 and p  >  0.05 ( not significant , n.s . ) . values are presented as means    the standard error on the mean ( sem ) of six experiments microvascular blood flow measured using laser doppler velocimetry in intestinal wall exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d 120  mmhg , e 150  mmhg , f 170  mmhg . the measurements were performed in an intestinal loop , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 and p  >  0.05 ( not significant , n.s . ) . values are presented as means    the standard error on the mean ( sem ) of six experiments the microvascular blood flow was measured in the omentum , sutured to the dressing . the blood flow was 1.4 ( 0.1 ) pu before and 1.3 ( 0.1 ) pu ( p  >  0.05 ) after application of 50  mmhg , and 1.6 ( 0.1 ) pu before and 1.5 ( 0.2 ) pu ( p  >  0.05 ) after application of 70  mmhg , and 1.4 ( 0.2 ) pu before and 1.2 ( 0.2 ) pu ( p  >  0.05 ) , after application of 100  mmhg , and 1.4 pu ( 0.1 ) before and 1.2 ( 0.2 ) pu ( p  >  0.05 ) after application of 120  mmhg , and 1.4 ( 0.1 ) pu before and 1.1 ( 0.1 ) pu ( * p  <  0.05 ) after application of 150  mmhg , and 1.4 ( 0.1 ) pu before and 0.9 ( 0.1 ) pu ( * p  <  0.05 ) after application of 170  mmhg.fig . 4microvascular blood flow measured using laser doppler velocimetry in omentum exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d ) 120  mmhg , e ) 150  mmhg , f ) 170  mmhg . the measurements were performed in the omentum , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 , and p  > values are presented as means    the standard error on the mean ( sem ) of six experiments microvascular blood flow measured using laser doppler velocimetry in omentum exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d ) 120  mmhg , e ) 150  mmhg , f ) 170  mmhg . the measurements were performed in the omentum , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 , and p  > values are presented as means    the standard error on the mean ( sem ) of six experiments the microvascular blood flow was measured in the small intestinal loop of the abdomen , sutured to the dressing . the blood flow was 2.7 ( 0.2 ) pu before and 2.0 ( 0.2 ) pu ( * p  <  0.05 ) after application of 50  mmhg , and 3.8 ( 0.4 ) pu before and 2,5 ( 0.2 ) pu ( * p  <  0.05 ) after application of 70  mmhg , and 4.0 ( 0.6 ) pu before and 2.7 ( 0.4 ) pu ( * * p  <  0.01 ) , after application of 100  mmhg , and 3.8 pu ( 0.6 ) before and 2.3 ( 0.3 ) pu ( * * p  < 0.01 ) after application of 120  mmhg , and 3.5 ( 0.6 ) pu before and 1.9 ( 0.3 ) pu ( * * p  < 0.01 ) after application of 150  mmhg , and 3.6 ( 0.6 ) pu before and 1.5 ( 0.2 ) pu ( * * p  < 0.01 ) after application of 170  mmhg.fig . 3microvascular blood flow measured using laser doppler velocimetry in intestinal wall exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d 120  mmhg , e 150  mmhg , f 170  mmhg . the measurements were performed in an intestinal loop , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 and p  >  0.05 ( not significant , n.s . ) . values are presented as means    the standard error on the mean ( sem ) of six experiments microvascular blood flow measured using laser doppler velocimetry in intestinal wall exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d 120  mmhg , e 150  mmhg , f 170  mmhg . the measurements were performed in an intestinal loop , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 and p  >  0.05 ( not significant , n.s . ) . values are presented as means    the standard error on the mean ( sem ) of six experiments the blood flow was 1.4 ( 0.1 ) pu before and 1.3 ( 0.1 ) pu ( p  >  0.05 ) after application of 50  mmhg , and 1.6 ( 0.1 ) pu before and 1.5 ( 0.2 ) pu ( p  >  0.05 ) after application of 70  mmhg , and 1.4 ( 0.2 ) pu before and 1.2 ( 0.2 ) pu ( p  >  0.05 ) , after application of 100  mmhg , and 1.4 pu ( 0.1 ) before and 1.2 ( 0.2 ) pu ( p  >  0.05 ) after application of 120  mmhg , and 1.4 ( 0.1 ) pu before and 1.1 ( 0.1 ) pu ( * p  <  0.05 ) after application of 150  mmhg , and 1.4 ( 0.1 ) pu before and 0.9 ( 0.1 ) pu ( * p  <  0.05 ) after application of 170  mmhg.fig . 4microvascular blood flow measured using laser doppler velocimetry in omentum exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d ) 120  mmhg , e ) 150  mmhg , f ) 170  mmhg . the measurements were performed in the omentum , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 , and p  > values are presented as means    the standard error on the mean ( sem ) of six experiments microvascular blood flow measured using laser doppler velocimetry in omentum exposed to npwt of a 50  mmhg , b 70  mmhg , c 100  mmhg , d ) 120  mmhg , e ) 150  mmhg , f ) 170  mmhg . the measurements were performed in the omentum , which had direct contact with the center of the vac pack bandage . significance was defined as p  <  0.05 , p  <  0.01 , p  <  0.001 , and p  > values are presented as means    the standard error on the mean ( sem ) of six experiments the management of open abdomen in severely injured patients or those with serious intra - abdominal infections represent a significant challenge to the surgeon and may include treatment of abdominal compartment syndrome , effects on respiration , cardiovascular and renal function , and even  damage control  laparatomy . life - sustaining emergency operations in patients with severe abdominal injuries are often accompanied by visceral edema , retroperitoneal hematoma , or packing of the abdominal cavity . the pressure of forced abdominal wall closure or an abdominal infection may lead to ischemia and necrosis of the abdominal fascia . the latter may result in abdominal rupture with subsequent development of an abdominal wall hernia [ 13 , 5 , 14 , 2024 ] . with the development of damage control techniques and the understanding of abdominal compartment syndrome , if the abdomen is not closed in the early postoperative period , the combination of adhesions and fascial retraction frequently make primary fascial closure impossible , and creation of a planned ventral hernia is often required . npwt employs suction applied to a large polyurethane sponge under an occlusive dressing in the wound and allows for constant medial traction of the abdominal fascia . the technique also allows the abdominal wall to move freely toward the midline without the interference of adhesions between the bowel and the abdominal wall . higher closure rates of the abdomen have been reported with npwt compared with other techniques [ 4 , 6 , 14 , 15 , 24 , 25 ] . however , the method has occasionally been associated with increased development of intestinal fistulae and enteroatmospheric fistulae [ 6 , 10 , 12 , 13 , 18 , 26 , 27 ] . the mechanical effects of npwt may result in ischemia , which in turn may promote the development of fistulae . in the present study , we show that the blood flow in the intestinal wall significantly decreases at all levels of npwt between 50 and 170  mmhg . the results also indicate that the decrease in blood flow escalates according to the force used . for example , the decrease in intestinal wall blood flow was greater when using 170 than 150  mmhg , and the decrease in intestinal wall blood flow was greater when using 150 than 120  mmhg and so on . the blood flow in the omentum was unchanged during application of npwt of 50 and 70  mmhg but was significantly decreased during application of npwt between 100 and 170  mmhg . after application of the npwt dressing , the animal was untouched for 30  min ( 0  mmhg was applied ) ; the microvascular blood flow of the small intestinal wall and the omentum were continuously measured . steady state was established after 3060  s. the decrease in blood flow could be witnessed instantly after the negative pressure was applied , steady state was seen after 3060  s , and recordings were made after approximately 15  min . no changes in microvascular blood flow in the intestinal wall and the omentum was seen after steady state was established in this time frame . we have previously shown that npwt induces an increase in the blood flow of the peristernal soft tissue ( i.e. , skeletal muscular and subcutaneous tissue ) and also that the change is related to local effects , since the blood flow at a distance of 4.5  cm from the wound edge was not affected by the negative pressure . the blood flow increased with elevated subatmospheric pressure in both subcutaneous and skeletal muscular tissue . when the area under the flow - distance curve was analyzed , covering 0.5 to 4.5  cm from the wound edge , a maximal net increase in blood flow was observed at 75 and 100  mmhg , in muscular tissue . a difference in the profiles of the blood flow responses the distance from the wound edge to the point at which the blood flow increased was shorter in muscular tissue than in subcutaneous tissue . this may indicate that pressure is transduced differently in soft and dense tissue , and a less dense tissue collapses more easily when affected by pressure . in the immediate proximity of the wound edge , a zone of relative hypoperfusion this zone was larger at high negative pressures and was especially prominent in subcutaneous tissue . the size of the hypoperfused zone depended on the pressure applied and expanded with increasing negative pressure . in summary , the changes in the peristernal wound blood flow caused by tnp vary with the distance from the wound edge . a few centimeters away from the wound edge , conversely , in the immediate proximity of the wound , the negative pressure induced relative hypoperfusion . theoretically , these physiological courses of events may also appear in the intestinal wall and in the omentum during exposure to tnp . subsequently , a large force of npwt could theoretically induce a large zone of ischemia in the intestinal wall and in the omentum that are in close contact with the vac pack dressing and could lead to the development of intestinal fistulae . another explanation of the present study result may be that the npwt of the open abdomen creates a form of hernia between the wound edges , and the hernia may increase with the amount of npwt applied . a serious complication associated with the use of npwt in cardiac surgery is right ventricle rupture , often resulting in death [ 29 , 30 ] . we have previously identified the cause of heart rupture in pigs using magnetic resonance imaging . the heart was shown to be drawn up towards the thoracic wall ; the right ventricle bulged into the space between the sternal edges , and the sharp edges of the sternum protruded into the anterior surface of the heart . placing multiple layers of paraffin gauze over the anterior portion of the heart however , these events could be prevented by inserting a flexible rigid plastic disc between the anterior part of the heart and the inside of the thoracic wall . when using npwt in the treatment of the open abdomen , it is possible that a similar mechanism occurs , i.e. , bulging of the small intestines into the space between the wound edges , which may in part explain the ischemia seen in the underlying intestinal wall during npwt of the open abdomen . in future studies , it would be of interest to study the intestinal microvascular blood flow in intestinal loops close to the anterior abdominal wall that are in contact with the visceral protective layer ; if no or less decrease in blood flow is seen , the hypothesis of a mechanic hernia is strengthened . in the present study , we show that applying negative pressure wound therapy to the open abdomen induces ischemia in the small intestines and the omentum . we have also shown that the decrease in blood flow increased with the amount of negative pressure applied . we speculate that a longstanding decreased blood flow in the intestinal wall may induce ischemia and secondary necrosis in the intestinal wall , which could promote the development of intestinal and enteroatmospheric fistulae . it is , however , uncertain what kind of clinical aspects these findings might have . furthermore , we believe that npwt of the open abdomen is a very effective treatment , mainly by its draining effects , but the treatment could probably be improved . we speculate that a kind of protective disc over the intestines could protect from local ischemia but still provide effective drainage .
purposehigher closure rates of the open abdomen have been reported with negative pressure wound therapy ( npwt ) compared with other wound therapy techniques . 
 however , the method has occasionally been associated with increased development of intestinal fistulae . 
 the present study measures microvascular blood flow in the intestinal wall and the omentum before and during npwt.methodssix pigs underwent midline incision and application of npwt to the open abdomen . 
 the microvascular blood flow in the underlying intestinal loop wall and the omentum was recorded before and after the application of npwt of 50 , 70 , 100 , 120 , 150 , and 170  mmhg respectively , using laser doppler velocimetry.resultsa significant decrease in microvascular blood flow was seen in the intestinal wall during application of all negative pressures levels . 
 the blood flow was 2.7 ( 0.2 ) perfusion units ( pu ) before and 2.0 ( 0.2 ) pu ( * p  <  0.05 ) after application of 50  mmhg , and 3.6 ( 0.6 ) pu before and 1.5 ( 0.2 ) pu ( * * p  <  0.01 ) after application of 170  mmhg.conclusionsin the present study , we show that negative pressures between 50 and 170  mmhg induce a significant decrease in the microvascular blood flow in the intestinal wall . 
 the decrease in blood flow increased with the amount of negative pressure applied . 
 one can only speculate that a longstanding decreased blood flow in the intestinal wall may induce ischemia and secondary necrosis in the intestinal wall , which , theoretically , could promote the development of intestinal fistulae . 
 we believe that npwt of the open abdomen is a very effective treatment but could probably be improved .