there are many composites available on the market , and they are continually developed to meet the needs of professionals , who require esthetic restoration materials with simple application techniques and good mechanical and physical properties . nevertheless , these materials are subject to frequent failures , such as marginal and/or body discoloration , in addition to micro - infiltration and porosity,1,2 which are determinant in the success of the treatment.3 approximately 30 to 40% of the restorations made with composite resin in anterior teeth are replaced within 5 years.4 color stability is an important property of composites used in esthetic restorations and several factors are associated with alterations of this property , such as the composition of the resin matrix and type of light curing unit used.510 another important component that interferes in this property is the photo - initiator system , commonly composed of camphorquinone and aromatic or aliphatic amines that also act as accelerators.11 all amines are known to form by - products during the light - cure process , leading to discoloration of the material when under the influence of light or heat.12 furthermore , exposure to uv radiation may cause oxidation of double carbon links to produce color compounds that contain peroxide.13 discoloration may also be of exogenous origin , mainly caused by the absorption of coloring solutions such as tea , coffee and mouth rinses.14 pulp - protection materials are extremely important for pulp health , affecting protection against thermal , electric and mechanical stimuli.15 nonetheless , depending on the cavity depth and the transparency of the restoration material , the color of the pulp - protection material may affect the end result of the esthetic restoration . at present , with the development of whitening techniques , teeth assume a lighter color and their restorations demand more translucent materials . thus , protection materials can interfere in the final esthetic result of the restoration.16 the aim of this study was to asses , in vitro , the color stability of composites placed over calcium hydroxide and glass ionomer cement pulp protectors when submitted to an accelerated artificial aging process . the hypothesis tested was that composites placed over protection materials present the greatest variations in color stability . for the development of this study , composites ( table 1 ) associated with two pulp - protection materials were used : hydro c ( dentsply , petroplis , rj , brazil ) , a calcium hydroxide - based ( ch ) , and vivaglass liner ( ivoclar vivadent , schaan , liechtenstein ) , a glass ionomer cement - based ( gic ) material . for each composite , 30 specimens were made in two layers , one with 0.5 mm of protection material and another with 2.5 mm of restoration material , with a total thickness of 3.0 mm , placed on a 12 mm - diameter teflon matrix . to standardize the thickness of each layer , spacers were adapted over the central axis of the matrix within the thicknesses determined for each layer . after adapting the 0.5 mm spacer , the protection materials were manipulated according to the manufacturers recommendations and inserted into the matrix until it was completely filled . after pressing , the 2.5 mm spacer was adapted over the central axis of the matrix and 1.0 mm and 1.5 mm increments of composite were applied and light cured with halogen light ( ultralux electronic , dabi atlante , ribeiro preto , sp , brazil , 400/500 nm , 350/500mw / cm ) for the time recommended by the manufacturer . the intensity of the light source ( mw / cm ) was measured periodically by a radiometer ( curing radiometer model 100 , demetron research corp . , a glass cover slip was placed over the last resin layer , to enable a flat surface to be obtained and to avoid oxygen inhibition . at the end of the light curing process , the samples were removed and stored in isolated receptacles in the absence of light at 37c for 24 hours . specimens were polished with abrasive paper discs in a descending order of abrasiveness ( soflex , 3 m espe , sumar , sp , brazil ) , with alternating intermittent movements , and wetting the surface to avoid overheating . after this they were separated into 3 groups ( n=10 ) , according to the type of protection used ( table 2 ) . the samples were submitted to the initial color reading against a white background block ( standard for 45 /0 reflectance and color gardner laboratory inc . bethesda , maryland 20014 ) using a spectrophotometer ( pcb 6807 byk gardner , geretsried , germany ) . the observation standard simulated by the equipment follows the cie l*a*b * system , which consists of two axes , a * and b * , which are at right angles and represent the dimension or color . the third axis , perpendicular to the a*b * plane , is the lightness l*.17 when it is activated , the spectrophotometer composed of 30 led lamps of 10 different colors , disposed in a circular arrangement light up , with the light beam incident on the material surface at an angle of 45 using the primary standard illuminant d65 , which simulates the spectrum of natural daylight . this beam is reflected back to the appliance at 0 , and thus , it captures and records the values of l * , a * and b * of each sample . the samples were submitted to accelerated artificial aging ( c - uv , comexim materias primas ltda , so paulo , sp , brazil ) under the action of uv light and condensed air - saturated water . the fixed working program consisted of 4 hours exposure to uv - b light at 50c and 4 hours condensation at 50c for a period of 384 hours,18 equivalent to 1 year of clinical service.19 according to ruyter et al20 color alteration occur in the first 300 hours of the aging process . after this procedure , the specimens were submitted to second color reading , and the color variation ( e ) was calculated by the following formula : e = [ ( l*)+ ( a*)+(b * ) ] , where l = final l *  initial l * ; a = final a *  initial a * and b = final b *  initial b*. the kolgomorov - smirnov test was applied to test the normal distribution of the data and the values were submitted to statistical analysis ( 2-way anova - bonferroni , significance level at 95% , factors of variation composite x protector material ) . figure 1 shows a comparison of the l * , a * and b * values obtained before aaa for each group tested . through the data analysis , it was detected that the samples placed on ch presented lower l * values than those placed on gic ( figure 1 ) , and were statistically significant ( p<.05 ) in comparison with the other groups for the quixfil composite . composites placed on gic presented slightly lower l * values than those obtained for the control group , without statistically significant differences ( p>.05 ) . furthermore , the protector exerted great influence on the b * coordinate ( which varies from blue to yellow ) , especially for the quixfil composite . nevertheless , when the two studied composites were placed on gic , both presented lower b * values than those of the ch group , which were statistically significant in comparison with the values of the other groups ( p<.05 ) . the e measurements obtained for each studied group and their respective standard deviations are shown in table 3 . the data show that concept was the material that presented the greatest e variation , in all groups , presenting statistically significant values when compared with quixfil , control and ch groups . when the behavior of concept was compared with regard to the type of protection material used , high e values were found for control samples ( without protection ) , with statistically significant results ( p<.05 ) in comparison with the protected groups that presented no differences between each other ( p>.05 ) . the quixfil composite did not present statistically significant differences ( p>.05 ) with regard to the protection material for any of the studied groups . when analyzing the results in table 3 , it is found that the results of e for the control group were higher than those for the groups with pulp - protection material , with statistically significant values for concept ( p<.05 ) . the increasing esthetic requirements of the patients , allied to the technological development of esthetic restoration materials , have led to an increase in their use . nonetheless , although they present good mechanical properties , professionals are concerned about the poor color stability of these materials ; especially in the long term.2022 instrumental analyses with the aid of a spectrophotometer have allowed objective results to be obtained . this method combined with accelerated artificial aging has routinely been used to study the esthetic properties of restoration materials.2330 studies conducted under accelerated aging conditions allow one to predict the physical behavior of dental composites . most polymers have functional groups in their molecular chains , which absorb ultraviolet light ; among them are the carbonyl group ( c = o ) , aromatic rings and double carbon links ( c = c).21 when a polymer molecule absorbs ultraviolet light through the functional groups , the energy leads to the structure being in a more unstable state . these groups can return to their original state in phases emitting the excess energy in a longer wavelength . if the excited molecule can not disperse the excess energy by some means , there will be a rupture ; that is , photochemical degradation . degradation that occurs due to aging , such as the loss of color or shine , loss of opacity , loss of adherence and the appearance of cracks is observed , and results from the action on the composite matrix in combination with the light - cure quality , method and light - cure time.12,21 moreover , aaa leads to the degradation of the matrix / silane / filler , since the composite absorbs the water that penetrates through the filler / resin interface , which leads to hydrolysis of the silane , reducing the retention of the filler particles.31 reference values established whether or not there was difference in color before aging , and demonstrated that the presence and type of protection material would alter the appearance of the restoration material . the l * axis reveals the amount of white there is in the color , so , the more white there is in the color , the greater is the l * value . thus , it was seen that the test specimens placed on glass ionomer cement were more luminous than those placed on calcium hydroxide , since glass ionomer is a white material when compared with calcium hydroxide , which has a dark - yellow color ( figure 1 ) and the final restoration would reflect less light , which gives a lower mean l * value . as regards the b * coordinate , the greater its value is , the closer the color of the material is to yellow . all the alterations in the coordinates of the cie lab scale will reflect on the e ( delta e ) values . consequently , the data confirm the hypothesis that pulp - protection materials directly interfere in the restoration color even before they are submitted to accelerated artificial aging . the size and volume percentage of inorganic particles could be factors that influence e after aaa;31 a microhybrid composite could be more transparent than hybrid composites after the surface degradation , so that the pulp protector was more visible . with a high percentage of inorganic filler in quixfil there would be more light reflected inside the material , resulting in lower transparency.32 the same phenomenon occurred in the samples without protection . both composites presented higher e values in comparison with the samples with pulp - protection material . the difference in light transmittance of the dental resin composites affected their clinical appearance.33 when light is illuminated on a translucent specimen backed by a glossy / white background , many fractions of light reaching the background may be reflected . however , when backed by a matt / black background or a light trap , the degree of reflection may be significantly reduced.34,35 with the absence of the protective material , the samples could present greater translucence , without the opacity of the protective material , providing greater light reflection through the material . there was no significant difference between the composites placed on gic ( table 3 ) , despite the high e values for concept . according ruyter et al20 concept and quixfil presented clinically unacceptable variations ( e3.3 ) in the control group ( 14.1 and 4.5 , respectively ) . the results proved the hypothesis that the pulp - protection materials interfere directly in the color of the restoration material and that the behavior of the composites differs over the course of time , according to the type of composite studied . according to the methodology used and the results obtained in this study , it could be concluded that : 
 pulp - protection material affected the original color of the composite before and after accelerated artificial aging;the effect of accelerated artificial aging differs , depending on the type of composite used ( hybrid or microhybrid);only quixfil placed on ch was within acceptable clinical parameters ( e<3.3 ) . pulp - protection material affected the original color of the composite before and after accelerated artificial aging ; the effect of accelerated artificial aging differs , depending on the type of composite used ( hybrid or microhybrid ) ; only quixfil placed on ch was within acceptable clinical parameters ( e<3.3 ) .
objectives : this study assessed the color stability of two composites associated with two pulp protectors submitted to accelerated artificial aging ( aaa).methods:60 test specimens were made with 0.5 mm of protection material ( calcium hydroxide - ch or glass ionomer cement - gic ) and 2.5 mm of restoration material ( concept or quixfil ) and divided into 3 groups ( n=10 ) according to the type of protection material / composite , and the control group ( no protection ) . after polishing , 
 color readings were obtained with a spectrophotometer ( pcb 6807 byk gardner ) before and after aaa for 384 hours , and l * , a * , and b * coordinates and total color variation ( e ) were analyzed ( 2-way anova , bonferroni , =05).results : composites placed on ch presented lower l * levels than those on gic , which presented higher l * values than the control group and lower b * values than those of the ch group . the concept composite presented higher e levels for all groups , differing statistically from quixfil , except when placed on gic.conclusions:it was concluded that the protection material could affect the color stability and aaa is a factor that enhances this effect , depending on the type of composite used .