patients with chronic kidney disease ( ckd ) often have decreased physical fitness and activity . the main causes are muscle atrophy , myopathy , inactivity , malnutrition , and lower albumin levels . besides , anemia , inflammation , and uremic acidosis also play a role . it was well demonstrated that increased urinary protein and albumin excretion are well - known risk factors for cardiovascular end - organ damage [ 8 , 9 ] . emerging data suggest that greater physical activity may be associated with less albuminuria and physical activity could protect against albuminuria [ 10 , 11 ] . however , the relationship between physical activity and albuminuria is not uniform . for example , in diabetic patients , physical activity is associated with lower albumin excretion , and physical activity has led to regression of albuminuria in interventional studies [ 12 , 13 ] . however , in nondiabetics these associations were not observed [ 14 , 15 ] . the relationship between physical activity and albumin / protein excretion transient proteinuria , including albuminuria , is common after intense exercise , and the prevalence ranges from 18% to 100% , depending on the type and intensity [ 16 , 17 ] . although all these data exist , there are few studies in the literature which specifically address the level of kidney function , urinary albumin excretion , and daily physical activity . thus the current study was aimed to investigate the relationship between daily physical activity , kidney function , and urinary albumin excretion . the subjects for this cross - sectional investigation were patients attending to the nephrology outpatient clinic in a state hospital for the first time or who are under followup . the study was in accordance with the declaration of helsinki and local approval and written informed consent was obtained from all patients . the demographic characteristics including age , sex , smoking status ( smoker or nonsmoker ) , and clinical characteristics ( presence of diabetes mellitus , presence of coronary artery disease , and the medications used ) were recorded . the exclusion criteria were as follows : type 1 diabetes , cerebrovascular disease , amputation history , presence of rhythm problems , uncontrolled hypertension , hyper- and hypothyroidism , chronic liver disease , regular analgesic use , symptomatic heart failure , fracture history , and a history of physical activity apart from normal daily activities in the last 24 hours . all patients underwent routine physical examination ; blood pressure ( bp ) measurements and 12-lead ecg and laboratory analysis . adequately sized cuffs ( standard cuff of 23  12  cm or a large cuff of 34  15  cm ) according to arm circumference were placed on the nondominant arm . the first and fifth phases of korotkoff sounds were taken as the systolic and diastolic bp , respectively . the measurements were taken after the patients had rested for 10  min in the sitting position with the arm comfortably placed at the heart level . each set of two measurements was averaged to give the office systolic and diastolic bps . routine biochemical analysis and 24-hour urine specimens were collected to determine creatinine clearance as well as protein and albumin excretion . an information leaflet along with a urine container was given to all subjects and they also received a verbal explanation about how to collect a proper 24-hour urine sample . after excluding the first morning urine sample of the collection day , urine was collected over 24  h , which included the first urine sample of the next morning . at the end of the collection period , the urine containers were taken to the laboratory within 24  h. adequacy of urine collection was determined by the predetermined standards . insulin resistance was calculated in diabetic patients by homeostasis model assessment ( homa - index ) by the following formula : 
 ( 1)(homa - index):[fasting  plasma  glucose(in  millimoles  per  liter )  fasting  serum  insulin(in  microunits  per  milliliter ) ]  (22.5)1 . after being given an explanation measurement of daily activities using nottingham extended activities of daily living scale ( neadls ) which was performed for each patient in interview . the neadls was originally designed in the uk , and it is also one of the most popular extended activities of daily living scales in rehabilitation centers . responses to all questions are evaluated as not performed , 0 point ; with help , 1 point ; on my own with difficulty , 2 points ; on my own easily , 3 points . for each subsection total , and with summation of all scores , final total neadls scores are obtained , and they range between 0 and 66 points . analysis of neadls score according to ckd stages was carried out by kruskal - wallis test . for the post hoc analysis of groups bonferroni corrected mann - whitney u test was performed . stepwise multiple linear regression was performed to identify independent variables ( age , gender , body mass index , presence of diabetes , presence of coronary artery disease , hemoglobin , albumin , 24-hour urinary albumin excretion ( uae ) , and 24-hour creatinine clearance ) related to logarithmically converted neadls score ( as a dependent variable ) . two patients with type 1 diabetes , 3 patients with cerebrovascular disease , 1 patient with amputation , 4 patients with rhythm problems , 2 patients with uncontrolled hypertension with 1 patient with hyperthyroidism , 2 patients with hypothyroidism , 2 patients with chronic liver disease , 7 patients with regular analgesic use , 2 patients with symptomatic heart failure , 3 patients with fracture history , and 5 patients with a history of physical activity apart from normal daily activities in the last 24 hours were excluded . twenty - nine patients had normal renal function , 20 patients have stage 1 ckd ( defined as creatinine clearance > 90  ml / min/1.73  m ) but have proteinuria / albuminuria and/or renal structural damage , 29 patients have stage 2 ckd ( defined as creatinine clearance between 60 and 89  min/1.73  m ) , 49 patients have stage 3 ckd ( defined as creatinine clearance between 30 and 59  min/1.73  m ) , 5 patients have stage 4 ckd ( defined as creatinine clearance between 15 and 29  min/1.73  m ) , and 7 patients have stage 5 ckd ( defined as creatinine < 15  min/1.73  m ) . the laboratory parameters of the patients were shown in table 2 . in whole group neadls score was correlated with age ( rho : 0.759 , p < 0.0001 ) , clinical sbp ( rho : 0.212 , p : 0.018 ) , blood urea nitrogen ( rho : 0.516 , p < 0.0001 ) , creatinine ( rho : 0.501 , p < 0.0001 ) , uric acid ( rho : 0.308 , p < 0.0001 ) , albumin ( rho : 0.382 , p < 0.0001 ) , total cholesterol ( rho : 0.194 , p : 0.022 ) , ldl - cholesterol ( rho : 0.230 , p : 0.008 ) , hemoglobin ( rho : 0.256 , p : 0.002 ) , creatinine clearance ( rho : 0.565 , p < 0.0001 ) , 24-hour urinary protein excretion ( rho : 0.324 , p < 0.0001 ) , and 24-hour uae ( rho : 0.483 , p < 0.0001 ) . scatter plot graphic between logarithmically converted neadls score with logarithmically converted 24-hour urinary albumin excretion and 24-hour creatinine clearance was shown in figures 1 and 2 , respectively . comparison of neadls scores among patients with normal renal function and ckd stages were shown in table 3 . subgroup analysis in 57 diabetic patients has shown that neadls score was correlated with age ( rho : 0.601 , p < 0.0001 ) , blood urea nitrogen ( rho : 0.401 , p : 0.002 ) , creatinine ( rho : 0.471 , p < 0.0001 ) , creatinine clearance ( rho : 0.502 , p < 0.0001 ) , albumin ( rho : 0.413 , p : 0.001 ) , 24-hour urinary protein excretion ( rho : 0.438 , p : 0.001 ) , and 24-hour urinary albumin excretion ( rho : 0.466 , p < 0.0001 ) . however , there was no relationship with hba1c and insulin levels and homa - index among diabetic patients . intact parathyroid hormone ( pth ) levels were available for 75 patients . in these group of patients neadls score was negatively associated with intact pth levels ( rho : 0.416 , p < 0.0001 ) . then multivariate linear regression of independent factors including age , gender , body mass index , presence of diabetes , presence of coronary artery disease , hemoglobin , albumin , 24-hour uae , and 24-hour creatinine clearance related to logarithmically converted neadls score ( as a dependent variable ) was carried out . of note 24-hour urinary protein excretion the current study investigated the independent relationship between daily physical activity with creatinine clearance and 24-hour uae . as a result it was demonstrated that 24-hour creatinine clearance was positively and 24-hour uae was negatively corelated with daily activity . these findings are novel since very few studies examined the relationship between 24-hour creatinine clearance and 24-hour uae with daily physical activity in a simultaneous way . recently , it was realized that both albuminuria and low glomerular filtration rate predict accelerated loss of kidney function , end - stage kidney failure , cardiovascular morbidity , and mortality [ 2022 ] . the current study also showed that both increased uae and decreased creatinine clearance are independently corelated with physical activity . thus apart from cardiovascular risk , physical activity was also influenced both by uae and creatinine clearance . previously , it was suggested that greater physical activity may be associated with less urinary albumin excretion . indeed , it was demonstrated that physical activity has protective effects on the vascular endothelium and endothelial dysfunction in the renal vasculature is associated with albuminuria . thus the relationship between increased albumin excretion and decreased physical activity may be explained in the context of endothelial dysfunction . another explanation may be the presence of microangiopathy and atherosclerosis [ 23 , 24 ] . besides , increased uae  as a measure of microangiopathy  has been suggested to influence lower limp function . it has also been suggested that atherosclerosis through chronic ischemia may lead to a disturbance of bone remodeling and to a loss of the mechanical properties of the bone [ 26 , 27 ] which may lead to decreased activity . last but not the least , increased oxidative stress and inflammation may be potential explanations regarding the relationship between increased uae and decreased physical activity . indeed , it was demonstrated that physical exercise reduces both oxidative stress and inflammation [ 2830 ] , which are both related with albuminuria . in the present study indeed , previous studies have shown that habitual physical activity was positively associated with renal function [ 3133 ] . why decrease in creatinine clearance was associated with decreased physical activity ? currently the exact mechanisms are unknown but speculations can be made . it is possible that progression of neuropathy by decreasing kidney function may contribute to a reduction in muscle mass with a consequent impact on mobility and physical activity . although not universal , some studies have shown that subjects with mild to moderate ckd may have increased risk of osteoporosis . also other bone abnormalities seen in ckd such as osteitis fibrosa or adynamic bone disease may result in diminished physical activity . in the current study in a subgroup of patients with intact pth levels being available , thus more studies are needed to highlight specific relationship between pth , bone histology , and physical activity in ckd patients . inflammation , acidosis , protein - energy malnutrition which are common in ckd patients may be other potential explanations given the fact that these conditions are aggravated with sedentary life and active daily life has vasculoprotective effects [ 7 , 35 , 36 ] . as an example it would be interesting to test whether drugs that decreased albumin excretion will also improve physical activity . on the other hand it should be determined whether increasing daily activity will improve kidney function . the present study has some limitations that deserve mention . as the study was cross - sectional temporality can not be suggested . besides the current findings might be interpreted with caution since associations may be 2-sided . on one hand it could be possible that patients who were physically active may have lower albuminuria values and better renal function . on the other hand , that patients with a better renal function ( not severe renal disease ) practice regular physical activity more easily than patients with more severe renal damage . thus cause and effect relationship can not be suggested in such a kind of study . second , despite multivariate adjustment for multiple potential confounders , there remains a possibility of residual confounding . given the fact that change in albumin excretion may occur even in the same individual , only one measurement of uae is another limitation . because physical activity can induce a transient increase in uae , the association between regular physical activity and albuminuria can be difficult to study . however , the patients with history of exercise apart from normal daily activity in the last 24 hours were excluded in the study . the current study also did not evaluate inflammation level and acidosis and alkalosis level . in conclusion , daily physical activity is independently corelated with both uae and kidney function .
the present study was carried out to investigate independent relationship between daily physical activity , microalbuminuria , and kidney function . 
 the demographic characteristics and laboratory parameters were recorded for all patients . 
 the determination of daily activities was carried out by nottingham extended activities of daily living scale ( neadls ) which was performed for each patient in an interview . 
 totally 139 patients were enrolled . in the whole group neadls score 
 was correlated with age ( rho : 0.759 , p < 0.0001 ) , clinical systolic blood pressure ( rho : 0.212 , p : 0.018 ) , blood urea nitrogen ( rho : 0.516 , p < 0.0001 ) , creatinine ( rho : 0.501 , p < 0.0001 ) , uric acid ( rho : 0.308 , p < 0.0001 ) , albumin ( rho : 0.382 , p < 0.0001 ) , total cholesterol ( rho : 0.194 , p : 0.022 ) , ldl - cholesterol ( rho : 0.230 , p : 0.008 ) , hemoglobin ( rho : 0.256 , p : 0.002 ) , creatinine clearance ( rho : 0.565 , p < 0.0001 ) , 24-hour urinary protein excretion ( rho : 0.324 , p < 0.0001 ) , and 24-hour urinary albumin excretion ( uae ) ( rho : 0.483 , p < 0.0001 ) . 
 the multivariate linear regression of independent factors corelated with logarithmically converted neadls score ( as a dependent variable ) has shown that age ( p < 0.0001 ) , presence of coronary artery disease ( p : 0.011 ) , hemoglobin ( p : 0.020 ) , 24-hour creatinine clearance ( p : 0.004 ) , and 24-hour urinary albumin excretion ( p < 0.0001 ) were independently corelated with neadls score . in conclusion , 
 both uae and kidney function were independently associated with daily physical activity .