the approach we took was to investigate how sphingomyelinase d ( smased ) , an enzyme that converts sphingomyelin to ceramide-1-phosphate , influences the gating and pharmacological sensitivities of the kv2.1 channel from rat brain 12 . lu and colleagues reported that this kv channel is particularly sensitive to the lipase 6 , with removal of the choline moiety of sphingomyelin causing the channel to open at voltages where it would otherwise be closed 6 . in our experiments , application of smased to the extracellular side of cells expressing kv2.1 causes a 30 mv shift of the conductance - voltage ( g  v ) relation ( fig 1a ) that develops over 1015 min and is not reversible over the time frame of our recordings ( > 1 hr ) , consistent with previous results 6 . deactivation of the channel following membrane repolarization is greatly slowed after smased treatment ( fig 1a ) , indicating that the lipid modification causes a pronounced stabilization of the open state of the channel . to explore whether the effects of smased result from interactions between lipids and the s1s4 voltage - sensing domain of the channel , we asked whether the lipase has similar effects on another protein containing an s1s4 voltage - sensing domain but lacking a pore domain . for these experiments we used the ciona intestinalis voltage sensitive phosphatase ( ci - vsp ) , which contains a cytoplasmic phosphatase domain rather than a transmembrane pore 15 , and monitored activation of its s1s4 voltage - sensing domain by measuring charge movement in response to membrane depolarization 1517 . extracellular application of smased to cells expressing ci - vsp produces an approximately 20 mv shift of the charge - voltage ( q - v ) relationship to more negative voltages ( fig 1b ) . as in the case of kv2.1 these results demonstrate that modification of sphingomyelin can influence the activity of s1s4 voltage - sensing domains in the absence of a pore domain . one interesting candidate for a region within s1s4 voltage - sensing domains that interacts with lipids is the s3b s4 paddle motif , a helix - turn - helix motif that moves in contact with the lipid membrane to sense changes in voltage 3,4,8,1822 . to explore the possibility that sphingomyelin interacts with the paddle motif , we measured the effects of smased on kv2.1 chimeras containing paddle motifs from a variety of different voltage - activated ion channels ( fig 1c , d ) . of the constructs we studied , those containing paddle motifs from the four different voltage - sensing domains of the rat skeletal muscle nav1.4 sodium channel23 were the most informative . these nav channel paddle motifs are capable of gating the kv2.1 channel and rendering the kv channel sensitive to nav channel toxins 21 , however , smased alters the g - v relations of the chimeras to different extents ( fig 1d ) . in particular , kv channels containing the paddle motif from domain i of nav1.4 are effectively insensitive to smased , suggesting that the paddle motifs determine the sensitivity of the channel to lipid modification . if the membrane lipids studied here actually bind to paddle motifs in kv channels , it is possible that the binding of other ligands at the protein - lipid interface would be influenced by lipid modification . because tarantula toxins partition into membranes 9,2426 ( supplementary fig 1 ) and bind to regions of the paddle motif facing the surrounding membrane 1,8,10,21,2729 , we investigated whether their interaction with the paddle motif is altered by lipid modification . we studied the effects of smased on the interaction of gxtx-1e 30 with the wt kv2.1 channel , vstx1 25,31 with a chimera produced by transplanting the s4 helix from kvap in kv2.1 ( kvap[s4 ] ) 8 , and protx - i 32 with the two nav paddle chimeras that are sensitive to the toxin 21 ( fig 2 ) . the lipid modification produces a 3.9-fold increase in the apparent affinity of gxtx-1e for kv2.1 ( fig 2a ) , and a 6.2-fold increase in that of vstx1 for the kvap chimera ( fig 2b ) . the effects of protx - i on the two nav paddle chimeras are particularly interesting , as the apparent affinity of the toxin is increased by only 50% for the domain ii chimera ( nav dii [ s3s4 ) ) , but by over 6-fold for the domain iv chimera ( nav div [ s3s4 ] ) ( fig 2c , d ) . these effects of smased on the interactions of tarantula toxins with paddle motifs demonstrate that the toxins can sense the lipid modification . the distinct effects of the lipase on protx - i interaction with the two nav paddle chimeras suggest that the modification does not simply alter the way these toxins interact with lipids , for example by altering the toxin concentration in the membrane , but that the toxins can actually detect difference in the way lipids interact with distinct paddle motifs . to confirm that partitioning of tarantula toxins is not altered by smased , we compared partitioning of i - gxtx-1e into intact oocyte membranes 9 before and after lipid modification . at a concentration of gxtx-1e ( 200 nm ) that completely inhibits opening of kv2.1 ( fig 2a ) , partitioning of the toxin previous studies have shown that tarantula toxins interact with the paddle motif in a specific fashion , with mutations in the paddle having pronounced effects on the apparent affinity of these toxins 8,10,21,26,27,29,33 . the idea that both lipids and toxins interact with the paddle motif raises the intriguing possibility that mutations in the paddle might alter toxin affinity by disrupting lipid - paddle interactions . in search of such mutations , we substituted each residue within the paddle motif of kv2.1 with ala and measured the apparent affinity of gxtx-1e for these channels in control membranes and after treatment with smased . mutations such as v282a and e277a decrease the apparent affinity of the toxin when studied in untreated membranes , but to a lesser extent after lipid modification ( fig 3a ) . in the case of v282a , the mutant weakens toxin affinity by over 18-fold under control conditions , but by only about 2-fold after treatment with the lipase . in this instance , the extent to which the mutation weakens toxin affinity is strikingly dependent on the lipids around the channel , suggesting that the mutation actually influences how lipids interact with the paddle motif . another way of looking at this result is that for the wt channel , lipid modification increases toxin affinity by only about 4-fold , but after the paddle mutation has weakened toxin binding , lipid modification now increases toxin affinity by over 30-fold ( fig 3a ; supplementary fig 2 ) . it is as if the lipid modification can correct the disruptive effects of the mutation ( see discussion ) . the e277a mutant is an example where the effects of the mutation on toxin affinity is moderately different when comparing control and smased - treated membranes , even though the mutant alters the affinity of the toxin by almost 150-fold when studied in control membranes ( fig 3a ) . in this instance , the mutant seems to weaken toxin affinity largely by altering a protein - protein interaction and the lipid modification can only modestly correct the effects of the mutation . the extent to which the lipid modification and paddle mutations are coupled can be evaluated for all mutations by plotting the mutation - induced perturbation in toxin affinity for control membranes ( kd / kd ) against that for smased - treated membranes ( fig 3b , c ) . f285a , i273a , s278a and l283a are examples where the lipid modification largely corrects the weakening of toxins affinity , as described above for v282 . in other cases , the effects of mutants are unaltered by lipid modification , regardless of whether the effects of the mutation on toxin affinity are small ( e.g. i297a , q286a , r290a ) or large ( e.g. s281a , r296a ) , indicating that there is specificity in the coupling between the lipid modification and the paddle mutations . to understand where on the paddle motif these lipid interactions occur , we calculated the free energy associated with the coupling between channel mutations and the lipid modification ( gcoupling ; supplementary fig 2 ) 34,35 and mapped them onto the x - ray structure of the kv2.1/kv1.2 paddle chimera 1 , a structure where the voltage sensor is activated and the pore is open . using a continuous color gradient between white and red to represent increasing |gcoupling| , we can see that mutations that are coupled with the lipid modification tend to concentrate in two regions of the paddle motif ( fig 4 ) . the first is on the surface of the s3b helix that projects out towards the surrounding bilayer ( fig 4a , b ) , and the second is on the surface of the s4 helix facing the s5 helix from the adjacent subunit ( fig 4c ) . residues at the interface between s3b and s4 helices are notable in that their mutation does not couple to the lipid modification ( fig 4d ) . further experiments using vst1 as a detector to measure coupling between s4 mutations in the kvap[s4 ] chimera and lipid modification identify a similar face of the s4 helix where coupling occurs ( supplementary figs . 3 and 4 and supplementary tables 3 and 4 ) , demonstrating that lipids interact with similar regions of s4 in distantly related kv channels . the goal of the present study was to explore the interaction of sphingomyelin with s1s4 voltage - sensing domains and the role of the lipid in the function and pharmacology of kv channels . our experiments with ci - vsp establish that modification of sphingomyelin can influence s1s4 voltage - sensing domains in the absence of a pore domain ( fig 1b ) , and the results with paddle chimeras point to interactions between the lipid and the paddle motif ( fig 2 ) . in testing the idea that sphingomyelin may act like a non - annular lipid and bind to the paddle motif smased treatment typically increases the apparent affinity of these toxins , but the extent varies greatly for different toxin - paddle combinations ( fig 2 ) , and in particular , for mutations on the paddle motif of kv2.1 ( fig 3 ) . the lipid modification does not influence the interaction of the toxin with the bulk membrane ( fig 2 ) , suggesting that the toxin is detecting a local alteration near where it binds to the paddle motif . these results demonstrate that the pharmacological sensitivity of an ion channel can be profoundly influenced by the identity of lipids around the channel and their interactions with specific structural motifs . it seems likely that the effects of smased are not simply the consequence of removing sphingomyelin because conversion of the lipid to ceramide-1-phosphate with smased or to ceramide with smasec has very different effects on the gating of kv2.1 . the former facilitates opening 6 ( fig 1 ) , whereas the latter creates a barrier to opening of the channel 7 . it is unlikely that the effects of the lipase on toxin affinity are secondary to the dissociation of sphingomyelin - rich lipid microdomains ( rafts ) because mutations in the paddle motif couple with the lipid modification ( fig 3 ) . in addition , dissociation of these microdomains with cholesterol depletion does not influence activation of kv2.1 channels 6,36 or sensitivity of the channel to tarantula toxins ( not shown ) . one way to explain the effect of lipid modification on toxin affinity would be to postulate that tarantula toxins bind to the paddle motif with higher affinity when sphingomyelin is bound ( fig 5 ; kd<kd ) ) . in this way , mutations could weaken toxin binding by disrupting lipid - paddle interactions rather than by altering a protein - protein interaction between toxin and channel ( fig 3a ) . in the context of this model , lipid modification could rescue high affinity toxin binding after mutations weaken lipid - paddle interactions either because the interaction of the modified lipid is stronger ( lower kl ) or because the toxin binds more strongly to paddles interacting with ceramide-1-phosphate compared to sphingomyelin . where on the paddle motif do the lipids bind ? our experiments using tarantula toxins as detectors uncovered surfaces of both the s3b and s4 helices within the paddle motif where mutations couple with the lipid modification ( fig 4 ) . interactions between lipids and the s3b helix are interesting because these might help to anchor the helix near the membrane surface , which would be consistent with studies showing that residues in s3b do not exhibit substantial changes in accessibility to water soluble agents during gating 3739 . interaction of lipids with the surface of s4 that faces s5 is an ideal location to maximize electrostatic interactions between phosphate lipid head groups and the outer s4 arg residues to stabilize the voltage sensor in an activated state 57 . these protein - lipid interactions at the interface between voltage - sensing and pore domains could explain why s5 mutants in this region exhibit hindered voltage sensor activation 40 even though the s4 and s5 helices do not pack together tightly in x - ray structures 1,41 . the presence of bound lipids between s4 and s5 is consistent with the general picture whereby non - annular lipids bind to membrane proteins at interfaces between transmembrane helices 13,14 . although structural information on sphingolipid - binding proteins is limited , helix - turn - helix motifs reminiscent of the paddle motifs found in voltage sensors have been proposed to bind these lipids 42,43 . our observations concerning the sensitivity of kv channel pharmacology to lipids fit nicely with the discovery that kv channel pharmacology can also be modulated by the mechanical properties of the membrane 10 . the important implication is that the pharmacology of the ion channel is not determined by the protein alone , but by the lipids in the surrounding membrane and how they interact with the channel protein . it will be fascinating to explore whether variations in the composition and properties of membranes in different subcellular compartments , cell types , or pathophysiological conditions , influence the gating properties and pharmacological sensitivities of voltage - activated ion channels . methods and any associated references are available in the online version of the paper at http://www.nature.com / nsmb/.
voltage - activated ion channels open and close in response to changes in voltage , a property that is essential for generating nerve impulses . 
 studies on voltage - activated potassium ( kv ) channels show that voltage - sensor activation is sensitive to the composition of lipids in the surrounding membrane . here 
 we explore the interaction of lipids with s1s4 voltage - sensing domains , and find that the conversion of the membrane lipid sphingomyelin to ceramide-1-phosphate alters voltage - sensor activation in an s1s4 voltage - sensing protein lacking an associated pore domain , and that the s3b 
 s4 paddle motif determines the effects of lipid modification on kv channels . using tarantula toxins that bind to paddle motifs within the membrane , we identify mutations in the paddle motif that weaken toxin binding by disrupting lipid - paddle interactions . 
 our results suggest that lipids bind to voltage - sensing domains and demonstrate that the pharmacological sensitivities of voltage - activated ion channels are influenced by the surrounding lipid membrane .