neurogenic bladder is considered an important risk factor for chronic renal failure.1 it is well established that the best way of preserving renal function is to allow the bladder to fill and be emptied regularly.2 clean intermittent catheterization has a critical role in the prevention of urinary tract infections ( utis ) and in reducing bladder pressure.3,4 anticholinergic agents help reducing bladder pressure and improve bladder capacity.5   the quantification of glomerular filtration rate ( gfr ) and the presence of microalbuminuria and metabolic acidosis are frequently utilized for the assessment of the glomerular and tubular functions in chronic kidney disease . the gfr , calculated from serum creatinine , and the evaluation of microalbuminuria , provide information on glomerular function . microalbuminuria has emerged as a risk marker for chronic renal disease and its use in the diagnosis of incipient chronic renal lesion of several causes has been stimulated.6 metabolic acidosis is a marker of tubular lesion and is commonly associated with renal function loss and also with obstructive uropathies.7   the aim of this study was to evaluate the evolution of glomerular and tubular renal function in patients with congenital neurogenic bladder treated by pediatric nephrologists , identifying factors related to worsening of renal function in order to suggest treatment modifications . we have retrospectively reviewed the clinical records of 58 children with congenital neurogenic bladder treated at  voiding dysfunction clinic  , of  instituto da criana do hospital das clinicas da faculdade de medicina da universidade de so paulo data were collected on : gender , age at presentation to our unit , followup period in our service , etiology of neurogenic bladder , reason of referral , previous and followup medical / surgical management , previous and followup episodes of treated utis , initial urodynamic evaluation and dmsa scintigraphy , initial and followup weight , height and blood pressure , initial and followup levels of serum creatinine , microalbuminuria , venous ph and serum bicarbonate . as this is a retrospective study , we have defined treated uti as every urinary tract infection episode registered as such in the patient 's records , either in the admission history or in the outpatient clinic followup , and for which antibiotic therapy was prescribed by the attending physician . zscores were calculated for weight and height and values between 2 and + 2 standard deviations were considered normal.8,9 gfr was established from serum creatinine , based on schwartz 's formula.10 microalbuminuria analysis was performed based on a single urine sample and was defined as values higher than 30  mg / g of creatinine.11 metabolic acidosis was defined as bicarbonate levels lower than 20  meq / l , in a minimum of 2 consecutive venous blood samples , or when on alkali therapy . a complementary analysis was performed regarding microalbuminuria : group without microalbuminuria : patients in whom microalbuminuria was absent in the initial and final evaluations , or children in whom microalbuminuria was present in the initial assessment but disappeared during the followup study period . the occurrence of metabolic acidosis was evaluated in initially non acidotic patients by kaplan meier curves . this analysis was done to identify factors possibly related to the start of metabolic acidosis . data were compared according to gender ( male / female ) , period of followup ( less than 6 years/ equal to or more than 6 years ) , age at presentation to our service ( less than 3 years of age/ equal to or more than 3 years of age ) , presence of renal parenchymal damage on initial dmsa scintigraphy ( yes / no ) and history of uti ( yes / no ) . another complementary analysis was performed for metabolic acidosis considering two groups of patients : group without metabolic acidosis : patients in whom acidosis was absent in the initial and final evaluations or in whom acidosis was present initially but disappeared during followup . group with metabolic acidosis : patients in whom acidosis existed in the first and final evaluations or in whom acidosis has developed during the followup study period and was present in the final assessment . the obtained results were described as means , standard deviations , medians , minimum and maximum values , or as frequencies and percentages . comparison between initial and final evaluations for continuous quantitative variables was done using the student 's ttest for paired samples . the association between dichotomous variables was performed considering the fisher 's exact test . for evaluation of patient 's outcomes and comparison of subgroups defined by dichotomous variables , comparison between initial and final evaluations for continuous quantitative variables was done using the student 's ttest for paired samples . the association between dichotomous variables was performed considering the fisher 's exact test . for evaluation of patient 's outcomes and comparison of subgroups defined by dichotomous variables , of the 58 children studied , 33 were females ( 56.9% ) . the mean age at presentation to our unit was 4.2  3.5 ( 012.8 ) years . the mean of followup period was 3.8  3.1 ( 0.5  13.5 ) years . the mean age at the final evaluation was 8.0  5.3 ( 0.7  19.2 ) years . the most frequent aetiology of neurogenic bladder was myelomeningocele ( mmc ) in 42 ( 71.4% ) , followed by sacral agenesis in 7 ( 12.1% ) , spina bifida occulta in 6 ( 10.4% ) , teratoma in 2 ( 3.4% ) and flaccid tetraparesis in 1 patient ( 1.7% ) . the main reason that led to referral to our service was recurrent uti in 48 ( 82.8% ) patients , enuresis in 5 ( 8.6% ) patients and urinary retention in 5(8.6% ) patients . none of the 58 children had been evaluated by nephrologists before referral to our service . therapeutic interventions for neurogenic bladder , performed before children arrived in our service and during followup are shown in table 1 . of the 49 patients with history of uti lack of improvement or increase in uti episodes occurred in 12 ( 20.7% ) patients ( table 2 ) . of the 58 children , 41 ( 70.7% ) had their first urodynamic evaluation performed after the arrival at our unit . in these children , bladder capacity was reduced in 23 ( 56.1% ) and detrusorsphincter dyssynergia was found in 6 ( 14.6% ) . of the 58 children , 45 ( 77.6% ) had their dmsa scintigraphy performed after the arrival at our unit and 24 ( 53.3% ) of these patients had renal scars . 1.5  2.1 (  6.96 to + 2.78 ) and  1.5  2.2 (  6.91 to + 2.53 ) respectively . mean zscore for initial and final height were 1.8  1.8 ( 5.27 to + 1.37 ) and  2.1  1.8 ( 6.19 to + 1.08 ) respectively . blood pressure was normal in 39 ( 68.4% ) patients in the initial evaluation and in 38 ( 65.5% ) in the final assessment . prehypertension was diagnosed in 7 ( 12.3% ) patients initially and in 2 ( 3.5% ) in the final assessment . hypertension was diagnosed in 12 ( 20.2% ) children in the initial evaluation and in 18 ( 31% ) in the final assessment . in the period before referral to our service all of the children who were diagnosed as hypertensive in our service were started on antihypertensive agents . when there was coexistence with microalbuminuria , angiotensin converting inhibitors ( ace inhibitors ) or angiotensin ii receptor1 blockers were preferred . mean initial and final gfr were 146.7  70.1  ml/1.73  m / min ( 50.2 to 445.5 ) and 193.6  93.6  ml/1.73  m / min ( 90.2 to 605 ) respectively . a significant difference between mean initial gfr and mean final gfr was found ( p  =  0.0004 ) ( figure 1 ) . microalbuminuria analysis was possible in 37 patients ( 63.8% ) initially and in 42 children ( 72.4% ) in the final assessment . microalbuminuria was found in 20 ( 54.1% ) patients at presentation and in 29 children ( 69.0% ) in the final assessment . none of patients with microalbuminuria at the time of presentation was taking medication on that account . treatment with ace inhibitors or angiotensin ii receptor1 blockers was initiated after identification of microalbuminuria in all of the patients . the comparison between the group without microalbuminuria and the group with microalbuminuria is on the table 3 , 4 and 5 . there was no difference regarding gender , aetiology of neurogenic bladder , history of uti , blood pressure , metabolic acidosis and number of urinary tract infections during follow up . there was also no significant difference with regard to age at presentation , age in the final assessment , period of followup , initial / final weight zscore , difference of weight zscore , initial / final height zscore , difference of height zscore , initial / final gfr , and difference of gfr . metabolic acidosis was found in 11 ( 19% ) patients initially and in 19 children ( 32.8% ) in the final evaluation . only 3 of the 11 patients with acidosis in the initial evaluation were on alkali therapy on referral to our service . all of the patients in whom acidosis was diagnosed in our service were started on sodium bicarbonate to maintain bicarbonate serum level  24  meq / l . the 47 patients who did not present metabolic acidosis in the initial evaluation were analyzed by kaplanmeier curves for duration of disease ( coincident with patient 's age ) . a significant difference was confirmed with regard to age at presentation ( children with less than 3 years of age versus children aged 3 or more ) . initiation of metabolic acidosis during the followup period was more frequent in the children who presented earlier to our service ( figure 2 ) . the development of metabolic acidosis did not present an association with male / female gender , duration of followup ( < 6 years/ >  =   6 years ) , presence / absence of history of uti , presence/ absence of microalbuminuria in the initial evaluation , presence / absence of parenchymal damage on dmsa scintigraphy . the comparison between the group without acidosis and the group with acidosis showed statistically significant lower values of final weight zscore ( p  =  0.048 ) , initial height zscore ( p  =  0.047 ) and final height zscore ( p  =  0.022 ) in the group without acidosis . there was no significant difference between these two groups regarding other studied variables : gender , aetiology of neurogenic bladder , history of uti , blood pressure , metabolic acidosis , number of uti episodes , age at presentation , age in final assessment , duration of followup , initial weight zscore , difference of weight zscore , difference of height / stature zscore , initial / final gfr , and difference of gfr ( tables 6 , 7 and 8) . a comprehensive renal function followup , preferably by a pediatric nephrologist , should be routinely performed in children with congenital neurogenic bladder starting early in the course of the disease , as they are at risk of developing permanent renal damage . unfortunately , in the present study , the first renal evaluation occurred at a mean of 4.2 years of age . at the time of presentation , 7 patients had metabolic acidosis without therapy , 7 patients were hypertensive without antihypertensive treatment and 20 patients demonstrated microalbuminuria without antiproteinuric therapy . ulsenheimer et al retrospectively analyzed 36 children with myelomeningocele in a brazilian university hospital and showed that only 45% had been submitted to an urinary tract evaluation.12 kari reported on 11 children with neurogenic bladder in saudi arabia whose mean age at followup initiation was 6.6 years , all of them had a gfr lower than 50  ml/1.73  m / min1 . dik et al4 presented data on 176 patients with spina bifida who had been referred to an university hospital in the newborn period and showed that an early start to therapy has helped to safeguard renal function . sacral agenesis is less common and is almost always associated with maternal insulindependent diabetes mellitus . we found that 71.4% children had mmc and 12.1% had sacral agenesis , in agreement with data from cass et al , who found 78.2% mmc and 4.4% sacral agenesis in 413 patients with neurogenic bladder.3   in our data , referral of 84.5% of children was motivated by uti . ghoniem et al showed data on 61 patients with mmc in whom history of uti was present in 70.5%.2 we have observed a reduction of the number of uti episodes after initiation of bladder catheterization , in agreement with the experience of cass et al.5   in the present study , the mean zscores for initial and final weight and for initial height were within the normal range , but the mean final height z score was below the expected levels , on account of five children with very low weight and height zscores , which had a negative impact in the overall final weight and height mean zscores . our data is in agreement with hayesallen13 who has studied children with mmc and observed that 88% were below the 10 percentile for height , and with rosenblum et al , who have studied 99 children with mmc and found that 50.5% were below the 3 percentile for height . some children had very high rates of gfr , such as 600  ml/1.73  m / min . and none presented a final gfr below 90  ml/1.73  m / min . in the present study , no association was evident between gfr and microalbuminuria or metabolic acidosis , or between , microalbuminuria and maintenance or increase of the number of uti episodes , in spite of the potential production of renal scarring , loss of renal parenchyma and secondary glomerulosclerosis which is related to repetitive episodes of uti . there was also no association between microalbuminuria and systemic hypertension , although hypertension can be a cause or a consequence of renal injury with evolution to hyperfiltration and microalbuminuria.15 the absence of these associations is probably related to the retrospective design of the study , in which children in different stages of kidney function compromise are compared as a group . it is unfortunate that the only evaluation of glomerular filtration rate that was available for this restrospective study is creatinine / creatinine clearance which may overestimate gfr in situations of low body muscle mass16 which is frequently the case of children with neurogenic bladder . sutherland in 199717 and del gado et al in 200317 demonstrated that children with mmc had smaller kidneys than healthy children , so in spite of the eventual bias of measuring gfr using creatinine as marker , the marked incidence of microalbuminuria in the described patients and its inherent association with hyperfiltration , a possible component of renal hipoplasia , which might explain the glomerular hyperfiltration verified in most of our patients at initiation and during clinical follow up , can not be discarded patients with obstructive uropathies may develop metabolic acidosis which can persist after decompression.7 two of the presently described patients recovered from acidosis after the relief of urinary obstruction . according to mingin et al19 lower values of serum bicarbonate may be associated with augmentation enterocystoplasty . of the 19 patients with acidosis , 4(21.1% ) have manifested a postaugmentation reduction of serum bicarbonate levels . it is interesting that the children who presented earlier to our service ( aged less than 3 years ) developed metabolic acidosis more often than the late referrals . a possible reason for that finding is that , as a reference university service , our unit cares for children with a more severe pattern of disease . in fact , all the presently described patients were clearly symptomatic at arrival , with history of urinary retention or utis , unfortunately we have not received any patient for purely preventive followup . the presence of metabolic acidosis is usually associated with low weight / height gain,20 but in our study the children who developed acidosis presented significantly higher final weight zscore and initial and final height zscores than the children without acidosis . a possible explanation for this finding is that we have used different criteria for diagnosing and treating metabolic acidosis . we have diagnosed metabolic acidosis with two consecutive venous serum bicarbonate below 20  meq / l but our treatment goal for metabolic acidosis was a bicarbonate serum level  24 we have made that option to alleviate the burden of the caretaker , for whom the introduction of an unnecessary medication , four times a day , may change compliance into burnout . our findings suggest that for the benefit of the patients ' growth and development , the bicarbonate level for diagnosis of metabolic acidosis should be increased . children with congenital neurogenic bladder were referred late for renal evaluation , with a mean age of 4.2 years . renal damage was evident in at least 53.3% of the patients on arrival to our service according to available dmsa scans . despite adequate management , a significant number of patients developed microalbuminuria and metabolic acidosis , with gfr above normal range suggesting hyperfiltration . the data of this study strongly indicate that these patients should have initiated treatment much earlier , preferably immediately after birth . close follow up of these patients is desirable in order to treat complications as soon as possible to prevent further renal damage .
aims : preservation of renal function in children with congenital neurogenic bladder is an important goal of treatment for the disease . 
 this study analyzed the evolution of renal function in patients with congenital neurogenic bladder.methods:we reviewed the records of 58 pediatric patients with respect to the following attributes : gender , age , etiology of neurogenic bladder , reason for referral , medical / surgical management , episodes of treated urinary tract infections , urodynamics , dmsa scintigraphy , weight , height , blood pressure , glomerular filtration rate , microalbuminuria and metabolic acidosis . 
 statistical analysis was performed , adopting the 5% significance level.results:the mean age at presentation was 4.2  3.5 years . 
 myelomeningocele was the most frequent etiology ( 71.4% ) . 
 recurrent urinary tract infection was the reason for referral in 82.8% of the patients . 
 recurrent urinary tract infections were diagnosed in 84.5% of the patients initially ; 83.7% of those patients experienced improvement during followup . 
 the initial mean glomerular filtration rate was 146.7  70.1 ml/1.73 m2/min , and the final mean was 193.6  93.6 ml/1.73 m2/min , p  =   0.0004 . 
 microalbuminuria was diagnosed in 54.1% of the patients initially and in 69% in the final evaluation . 
 metabolic acidosis was present in 19% of the patients initially and in 32.8% in the final assessment.conclusions:patient referral to a pediatric nephrologist was late . 
 a reduction in the number of urinary tract infections was observed with adequate treatment , but microalbuminuria and metabolic acidosis occurred frequently despite adequate management .