wistar strain rats , with the weight of 150 - 250 grams , were acclimatized for one week under controlled conditions in a temporary animal house with access to food and water ad libitum . all the experiments performed in this study have been carried out according to the world medical association statement on animal use in biomedical research.25 the animals were then transported to the hbo unit at the time of operation and categorized into four groups : group 1 ( 15 ml saline gavage , n = 10 ) ; group 2 ( 11.5 mg / kg of powdered alp2627 in 15 ml saline gavage , n = 15 ) ; group 3 ( 11.5 mg / kg of powdered alp in 15ml saline gavage , n = 15 , this group was also under hyperbaric condition by compressed air at 1.4 - 2 atmosphere absolute ( ata ) ) ; and group 4 ( 11.5 mg / kg of powdered alp in 15 ml saline gavage , n = 15 , they also underwent hyperbaric condition by oxygen at 1.4 - 2 ata ) . the chamber was pressurized to 1.4 ata in 10 minutes and to 2 ata in 15 minutes . an isobaric stage of 30 minutes was selected for all animals and the chamber was decompressed to surface pressure within 20 - 30 minutes . survived animals were then transferred to another room . they were continuously observed for 5 days by two cameras that recorded all the events in the chamber . ( groups 2 - 4 ) , alp dissolved in normal saline was administered intragastrically by using gavage syringe and oral devices . since powdered phostoxin was used as alp source , we utilized an extra 5 ml of saline for the powder to dissolve better . although the rats were not permitted to be fed in the chamber , the survivors were fed with food and water ad libitum . alp ( phostoxin , 3 mg tablet , alp 56% ) , alcan , romania the chamber used in this study was a type b hyperbaric chamber with the characteristics as follows : a cylindrical body made of stainless steel with polymethyl methacrylic acid ( pmma ) viewports operating by compressed air or oxygen allowed either completely open or semi - closed modes using co2 remover . considering the potential risk of phosphine gas especially in oxygen enriched environments , a second chamber made of polytetrafluoroethylene ( ptfe ) was designed . the exposure of animals to hyperbaric oxygen was done by placing the second chamber in the main chamber . the main chamber was injected by compressed air and the second chamber was ventilated by oxygen at a rate of 50 lpm . the time for changing the profile of hbo treatment occurred when the first rat in group 2 died . at this point , isobaric condition was sustained by ventilation of 100 lpm of compressed air in groups 3 and 100 lpm of oxygen in group 4 . this monoplace hyperbaric chamber was completely designed , fabricated and installed as a laboratory instrument by the researchers at rasul - e - akram medical center to be used in a series of studies . at the time of the study , the chamber was under examination of inspectors from iranian ministry of health in search for its technical capabilities . the survival times of different groups were compared using log rank test of kaplan - meier analysis . survival times of different groups were noted using estimated means ( minutes )  standard error ( sem ) . wistar strain rats , with the weight of 150 - 250 grams , were acclimatized for one week under controlled conditions in a temporary animal house with access to food and water ad libitum . all the experiments performed in this study have been carried out according to the world medical association statement on animal use in biomedical research.25 the animals were then transported to the hbo unit at the time of operation and categorized into four groups : group 1 ( 15 ml saline gavage , n = 10 ) ; group 2 ( 11.5 mg / kg of powdered alp2627 in 15 ml saline gavage , n = 15 ) ; group 3 ( 11.5 mg / kg of powdered alp in 15ml saline gavage , n = 15 , this group was also under hyperbaric condition by compressed air at 1.4 - 2 atmosphere absolute ( ata ) ) ; and group 4 ( 11.5 mg / kg of powdered alp in 15 ml saline gavage , n = 15 , they also underwent hyperbaric condition by oxygen at 1.4 - 2 ata ) . the chamber was pressurized to 1.4 ata in 10 minutes and to 2 ata in 15 minutes . an isobaric stage of 30 minutes was selected for all animals and the chamber was decompressed to surface pressure within 20 - 30 minutes . survived animals were then transferred to another room . they were continuously observed for 5 days by two cameras that recorded all the events in the chamber . ( groups 2 - 4 ) , alp dissolved in normal saline was administered intragastrically by using gavage syringe and oral devices . since powdered phostoxin was used as alp source , we utilized an extra 5 ml of saline for the powder to dissolve better . although the rats were not permitted to be fed in the chamber , the survivors were fed with food and water ad libitum . alp ( phostoxin , 3 mg tablet , alp 56% ) , alcan , romania the chamber used in this study was a type b hyperbaric chamber with the characteristics as follows : a cylindrical body made of stainless steel with polymethyl methacrylic acid ( pmma ) viewports operating by compressed air or oxygen allowed either completely open or semi - closed modes using co2 remover . considering the potential risk of phosphine gas especially in oxygen enriched environments , a second chamber made of polytetrafluoroethylene ( ptfe ) was designed . the exposure of animals to hyperbaric oxygen was done by placing the second chamber in the main chamber . the main chamber was injected by compressed air and the second chamber was ventilated by oxygen at a rate of 50 lpm . the time for changing the profile of hbo treatment occurred when the first rat in group 2 died . at this point , isobaric condition was sustained by ventilation of 100 lpm of compressed air in groups 3 and 100 lpm of oxygen in group 4 . this monoplace hyperbaric chamber was completely designed , fabricated and installed as a laboratory instrument by the researchers at rasul - e - akram medical center to be used in a series of studies . at the time of the study , the chamber was under examination of inspectors from iranian ministry of health in search for its technical capabilities . the survival times of different groups were compared using log rank test of kaplan - meier analysis . survival times of different groups were noted using estimated means ( minutes )  standard error ( sem ) . wistar strain rats , with the weight of 150 - 250 grams , were acclimatized for one week under controlled conditions in a temporary animal house with access to food and water ad libitum . all the experiments performed in this study have been carried out according to the world medical association statement on animal use in biomedical research.25 the animals were then transported to the hbo unit at the time of operation and categorized into four groups : group 1 ( 15 ml saline gavage , n = 10 ) ; group 2 ( 11.5 mg / kg of powdered alp2627 in 15 ml saline gavage , n = 15 ) ; group 3 ( 11.5 mg / kg of powdered alp in 15ml saline gavage , n = 15 , this group was also under hyperbaric condition by compressed air at 1.4 - 2 atmosphere absolute ( ata ) ) ; and group 4 ( 11.5 mg / kg of powdered alp in 15 ml saline gavage , n = 15 , they also underwent hyperbaric condition by oxygen at 1.4 - 2 ata ) . the chamber was pressurized to 1.4 ata in 10 minutes and to 2 ata in 15 minutes . an isobaric stage of 30 minutes was selected for all animals and the chamber was decompressed to surface pressure within 20 - 30 minutes . survived animals were then transferred to another room . they were continuously observed for 5 days by two cameras that recorded all the events in the chamber . ( groups 2 - 4 ) , alp dissolved in normal saline was administered intragastrically by using gavage syringe and oral devices . since powdered phostoxin was used as alp source , we utilized an extra 5 ml of saline for the powder to dissolve better . although the rats were not permitted to be fed in the chamber , the survivors were fed with food and water ad libitum . the chamber used in this study was a type b hyperbaric chamber with the characteristics as follows : a cylindrical body made of stainless steel with polymethyl methacrylic acid ( pmma ) viewports operating by compressed air or oxygen allowed either completely open or semi - closed modes using co2 remover . considering the potential risk of phosphine gas especially in oxygen enriched environments , a second chamber made of polytetrafluoroethylene ( ptfe ) was designed . the exposure of animals to hyperbaric oxygen was done by placing the second chamber in the main chamber . the main chamber was injected by compressed air and the second chamber was ventilated by oxygen at a rate of 50 lpm . the time for changing the profile of hbo treatment occurred when the first rat in group 2 died . at this point , isobaric condition was sustained by ventilation of 100 lpm of compressed air in groups 3 and 100 lpm of oxygen in group 4 . this monoplace hyperbaric chamber was completely designed , fabricated and installed as a laboratory instrument by the researchers at rasul - e - akram medical center to be used in a series of studies . at the time of the study , the chamber was under examination of inspectors from iranian ministry of health in search for its technical capabilities . the survival times of different groups were compared using log rank test of kaplan - meier analysis . the p value of less than 0.05 was considered significant . survival times of different groups were noted using estimated means ( minutes )  standard error ( sem ) . all the animals in group 1 survived and all in the other three groups died within 5 days . the mean survival time of alp poisoned rats without any intervention was 91  1 minutes . among the intoxicated rats which underwent hyperbaric condition with compressed air at 1.4 - 2 ata ( group 3 ) , the maximum survival time was 330 minutes . the mean survival time for rats in group 3 was 262  8 minutes . in group 4 , the group under hyperbaric oxygenation with oxygen , the mean survival time was 276  6 minutes . the maximum survival time in poisoned rats of group 4 was 332 minutes ( figure 1 ) . in survival analysis , there was a significant difference between group 2 which received alp and the groups which underwent intervention ( groups 2 and 3 , p < 0.001 ; groups 2 and 4 , p we also found that the difference between the mean survival time in animals under either hyperbaric oxygen or compressed air was not significant ( p > 0.05 ) . alp poisoning is commonly used for suicidal attempts with a high mortality rate in iran and other countries , especially in developing countries.2832 the results of the present study showed that hyperbaric oxygen therapy may increase survival in alp poisoned rats but there was no significant difference between pure o2 and compressed air . despite over a century of use in medical settings , hyperbaric oxygen remains a controversial therapy . the last 20 years have seen a clarification of the mechanism of action of hyperbaric therapy and a greater understanding of its potential benefit.33 there have been some reports about methemoglobinemia occurrence in acute alp poisoned victims.16212634 methemoglobinemia is a rare clinical presentation in acute alp poisoning that may complicate the course of poisoning.2126 generalized cyanosis in the presence of normal arterial oxygen tension and failure of the cyanosis to resolve with oxygen therapy is an important diagnostic clue which almost always represents methemoglobinemia . ph3 and arsine are chemically very similar and thus the fact that methemoglobinemia may occur by both of them is not surprising.27 the other mechanism of action of ph3-induced methemoglobinemia is induction of free radicals.620 mostafazadeh et al . found a significant association between blood level of methemoglobin and mortality in patients with alp intoxication.26 decreased capacity of methemoglobin to deliver enough oxygen to tissues may be another cause of multiple organ failure following alp intoxication . moreover , intravenous hemolysis has also been reported due to alp intoxication which may have additional effects on impaired oxygen delivery to the target tissues.35 in other word , methemoglobinemia is a condition in which an abnormal proportion of the iron in heme moiety of the hemoglobin is oxidized to the ferric state leading to impaired oxygen transport and anemic hypoxia . in this situation , the oxygen dissociation curve of the unaffected hemoglobin shifts to the left.21 in previous studies , there have been some controversies about the effectiveness of methylene blue in treatment of methemoglobinemia in alp poisoned victims.62126 in one study , it was mentioned that hyperbaric oxygen therapy would be useful in management of alp poisoning.21 phosphine is thought to have the central role in mechanism of alp toxicity as it inhibits cytochrome c oxidase.3 alp toxicity can inhibit cytochrome c oxidase , and there are similarities between alp and some other chemicals such as cyanide that binds to the enzyme cytochrome oxidase aa3 similar to carbon monoxide and blocks the mitochondrial respiration chain which in turn causes depletion of adenosine triphosphate . the mechanism of toxicity is disruption of the electron transport chain in mitochondria , resulting in intracellular hypoxia.36 this disturbance in oxygen delivery affects the human machine functionally and then structurally . loss of consciousness and neurologic damage that occur by inhalation of 100% n2 , high levels of h2s , hcn , or co are due to binding to cytochrome a3 and stopping the oxidative flow of electrons and subsequent cellular hypoxia.22 considering the unique mechanism of toxicity , there have been many studies showing usefulness of hyperbaric treatment in co , hydrogen sulfide , h2s and ccl4 poisoning both in human and animal models . for moderate to severe co poisoning , hyperbaric oxygen is the treatment of choice.37 bitterman et al . found that in rats poisoned with ld75 hydrogen sulfide , pure oxygen at 1ata was effective in preventing death.38 gunn and wong reported five patients with severe h2s poisoning who were treated successfully with hbo.39 goldenberg et al . suggested hbo therapy to reduce mortality in h2s poisoned patients.36 animal experimental studies on the effects of hbo in ccl4 poisoning showed lower mortality rates and less impairment of the liver function.40 mininberg and kvetnoi studied the effects of hbo on rats with hepatotoxicity induced by carbon tetrachloride . they found a reparative process , absence of necrosis , and increased activity of the liver chromaffin cells in the liver tissue of treated animals.23 lawson et al . studied other mechanisms at work and suggested phosphine or its products to be likely to have some role by formation of organophosphines as a precursor for synthesis of organophosphates.41 prolongation of the survival of the animals poisoned with phosphoorganic compounds was reported by savateev et al . when they used hbo at 3 ata for 2 - 4 hours.42 although our findings showed hbo to be able to prolong survival time after intoxication , it will not decrease mortality rate in alp toxicity in rat model . even after hbo treatment process , a higher mortality rate in alp toxicity than that of other intoxications indicates that the mechanism of action of alp may be more complex and not just simply inhibiting cytochrome oxidase . in addition , such inhibition may be much more potent and eventually more lethal . to the best of our knowledge , more studies incorporating gastro - intestinal decontamination methods and other drugs that are known to protect mitochondrial oxidative phosphorylation such as nac19 , hydroxycobalamine and vitamin c should be conducted . lack of special devices such as type c hyperbaric chamber and advanced monitoring equipments , as well as unavailability of bio - enzyme assays ( plasma cholinesterase level , myocardial malonyldialdehyde , catalase , glutathione peroxidase ) , were some of the limitations of this study that could be considered by researchers in future works . this research was financially supported by research deputy of tehran university of medical sciences , tehran , iran ( project no . lack of special devices such as type c hyperbaric chamber and advanced monitoring equipments , as well as unavailability of bio - enzyme assays ( plasma cholinesterase level , myocardial malonyldialdehyde , catalase , glutathione peroxidase ) , were some of the limitations of this study that could be considered by researchers in future works . this research was financially supported by research deputy of tehran university of medical sciences , tehran , iran ( project no . lack of special devices such as type c hyperbaric chamber and advanced monitoring equipments , as well as unavailability of bio - enzyme assays ( plasma cholinesterase level , myocardial malonyldialdehyde , catalase , glutathione peroxidase ) , were some of the limitations of this study that could be considered by researchers in future works . this research was financially supported by research deputy of tehran university of medical sciences , tehran , iran ( project no . all authors contributed in proposal writing , performing the experiments , data analysis , and writing the final manuscript .
background : aluminium phosphide ( alp ) is used as a fumigant . 
 it produces phosphine gas which is a mitochondrial poison . 
 although this poisoning has been repeatedly reported in literature with a high mortality rate , there is no known antidote for alp intoxication . in the present study 
 , we studied the effects of hyperbaric oxygenation ( hbo ) on the survival time of alp intoxicated rats.methods:intoxicated rats with alp ( 11.5 mg / kg , oral gavage ) were placed in hyperbaric oxygenation with different concentrations of compressed air and oxygen.results:all the animals exposed to alp died within 5 days . 
 the mean survival times of rats exposed to alp without any intervention , treated with hyperbaric condition by compressed air , and treated with hyperbaric condition by pure o2 were 91  1 , 262  8 , and 276  6 minutes , respectively . in analysis of survival times , there was a significant difference between group 2 which received alp and the groups which underwent intervention ( groups 2 and 3 , p < 0.001 ; groups 2 and 4 , p < 0.001).conclusions : hyperbaric oxygenation may probably improve the survival time of the intoxicated rats with aluminium phosphide , but it may not decrease the mortality rate .