diabetes is one of the most common diseases and its incidence is rapidly increasing . according to the 2015 edition of the idf diabetes atlas , yearly published by the international diabetes federation , there are 415 million people worldwide with diabetes and this number is expected to grow up to 642 million in 2040 , with an estimated 55% increase in 25 years.1 the diffusion of diabetes is not uniform in population . in the wealthiest countries , it mostly affects people > 50 years of age , whereas it is more widespread among young people in middle - income countries.2 in 2015 , ~5 million deaths of people could be ascribed to diabetes , mostly related to the comorbidities of the disease and its complications.1 among the chronic complications of diabetes , the diabetic foot ( df ) , a chronic ulcerative condition , is the most prevalent and severe because it affects 25% of patients at least once in their life with a survival expectation less than that of many forms of cancer.3 df ulcers are correlated to several risk factors , such as neuropathy ( 86% of cases ) , peripheral arterial disease ( 49% of cases ) , minor foot trauma , and foot deformity.4 the risk of amputation is not negligible ; df is the major cause of lower extremity amputations ( leas ) with a risk 20-fold higher than that of the general population and a limb amputated every 30 seconds.5 the healing rate of df ulcers , defined as the rate of ulcers completely re - epithelized , stable for at least 2 weeks varies according to their pathogenesis,6 whereas the incidence is , depending on the reference population , 3%5% for major amputations and 5%40% for minor amputations . another study reported that amputations range from 2.1 to 13.7 per 1,000.79 the association of ischemia with infection increases 300% the risk of leas in df ulcer patients , whereas df ulcers precede leas in > 85% of the cases.10 the impact of such ulcers on the patients quality of life is high , as they impair both personal and work relationships , with heavy psychological consequences.11 in the united states , a substantial burden between $ 9 billion and $ 13 billion can be estimated for the health care system , summing the impact on private and public payers.12 infection in df ulcers is a major risk factor leading to amputation,10 and for this reason , the monitoring of the foot ulcers status may be of a great importance to ensure the effectiveness of therapies and the safety of patients . swan - icare ( smart wearable and autonomous negative pressure device for wound monitoring and therapy ) is a large - scale integrating european project seventh framework program that tackles the challenge of developing wearable devices and sensors to monitor and manage foot and venous ulcers.13,14 the objective is to deliver a personalized therapy to patients whose ulcer status is remotely monitored so that a prompt action can be put in place in case of infection . the patient does not need to be hospitalized and can continue his / her normal life while a set of wearable sensors transmits the ulcer condition to a database accessible by clinicians . the sensors will monitor parameters such as transepidermal water loss of the perilesional skin , glucose , body temperature , skin and ulcer temperature , spo2 , levels of matrix metalloproteinases and bacteria , and the ph of the ulcer . there is a general consensus that there is a strong relationship between the ulcer temperature and the degradation of the ulcer status.1519 in fact , an increase in the basal temperature can be a warning of the ulcer onset even 1 week before it starts.20 besides temperature , an increase of ph at the wound can also be a sign of infection as it is related to the presence of bacteria that proliferate in the ulcer.15,2125 high precision handheld infrared thermographic scanners and cameras can be used to measure ulcer temperature . similar consideration can be made for the measurement of ph as the market only offers handheld ph meters . this study describes the technologies and principles of the temperature and ph sensors that are under development in the swan - icare project . we report the sensing materials under investigation and their early performances for a future use to fabricate wearable sensors for monitoring df ulcers . a temperature - sensitive nanocomposite material was prepared consisting of a conducting phase of multiwalled carbon nanotubes ( mwcnts , baytubes c150 p ) dispersed in toluene within an insulating phase of a thermoplastic elastomer , poly(styrene - b-(ethylene - co - butylene)-b - styrene ) ( sebs , europrene sol th 212 ) . in these kinds of materials , temperature variations lead to changes in the electrical resistance , so that they can be used to fabricate temperature sensors.2629 a digital multimeter was used for log resistance values ( model 2700 , keithley ) and a thermostatic bath ( f 32 , julabo ) as a reference temperature . a dispersion of mwcnts  sebs was obtained as reported in a study by matzeu et al.26 a volume equal to 10 l of the dispersion was drop casted onto a pair of gold electrodes ( length of 7 mm ) and let dry at room temperature to obtain a film ( figure 1 ) . the electrodes were fabricated onto a kapton substrate ( thickness of 50 m ; cad line , pisa , italy ) . copper tracks were obtained by photolithography and electroplated with nickel and gold to fabricate the electrodes ( thickness of copper 35 m , nickel 3.0 m , and gold 1.2 m ) . . this treatment decreases sensitivity but improves stability , that has been identified as a major problem in the previous study,30 resulting in more accurate measurements.31 we exploited the potentiality of graphene oxide ( go , graphenea ) to measure ph because this material changes its potential in water solution when the ph changes.32 
 figure 2 shows the substrate used for the tests . a go layer coated the working electrode ( we ) of a screen printed - board ( c220bt , 33100.5 mm , dropsens ) . this board has gold counter electrode ( ce ) and we ( diameter 4 mm ) , whereas the reference electrode ( re ) consists of ag / agcl . the ph was measured as the change of the open circuit potential between the we and the re ( palmsens3 , palmsens ) . five microliters of a go dispersion in water was deposited onto the we and let dry at room temperature . the sensor was compared with a commercial ph meter ( basic 20 by crison with micro p glass electrode by xs sensor ) in hank s balanced salt solutions ( hbss ; sigma - aldrich ) . a temperature - sensitive nanocomposite material was prepared consisting of a conducting phase of multiwalled carbon nanotubes ( mwcnts , baytubes c150 p ) dispersed in toluene within an insulating phase of a thermoplastic elastomer , poly(styrene - b-(ethylene - co - butylene)-b - styrene ) ( sebs , europrene sol th 212 ) . in these kinds of materials , temperature variations lead to changes in the electrical resistance , so that they can be used to fabricate temperature sensors.2629 a digital multimeter was used for log resistance values ( model 2700 , keithley ) and a thermostatic bath ( f 32 , julabo ) as a reference temperature . a dispersion of mwcnts  sebs was obtained as reported in a study by matzeu et al.26 a volume equal to 10 l of the dispersion was drop casted onto a pair of gold electrodes ( length of 7 mm ) and let dry at room temperature to obtain a film ( figure 1 ) . the electrodes were fabricated onto a kapton substrate ( thickness of 50 m ; cad line , pisa , italy ) . copper tracks were obtained by photolithography and electroplated with nickel and gold to fabricate the electrodes ( thickness of copper 35 m , nickel 3.0 m , and gold 1.2 m ) . . this treatment decreases sensitivity but improves stability , that has been identified as a major problem in the previous study,30 resulting in more accurate measurements.31 we exploited the potentiality of graphene oxide ( go , graphenea ) to measure ph because this material changes its potential in water solution when the ph changes.32 
 figure 2 shows the substrate used for the tests . a go layer coated the working electrode ( we ) of a screen printed - board ( c220bt , 33100.5 mm , dropsens ) . this board has gold counter electrode ( ce ) and we ( diameter 4 mm ) , whereas the reference electrode ( re ) consists of ag / agcl . the ph was measured as the change of the open circuit potential between the we and the re ( palmsens3 , palmsens ) . five microliters of a go dispersion in water was deposited onto the we and let dry at room temperature . the sensor was compared with a commercial ph meter ( basic 20 by crison with micro p glass electrode by xs sensor ) in hank s balanced salt solutions ( hbss ; sigma - aldrich ) . as sebs is an insulating thermoplastic elastomer that expands or shrinks with temperature , this effect lead to changes in the topology of the mwcnt network . different behaviors were observed in different kinds of carbon nanotubes ( eg , zigzag and armchair),33 but the mwcnts showed a semiconductor behavior with a decrease of resistance at increasing temperatures . in the second place , the electrical resistance changed due to the different thermal expansion coefficients of the insulating phase ( polymer matrix ) and the conducting phase ( mwcnts ) , that led to a rearrangement of the topology of the network at different temperatures . sebs shows a small but significant percentage of crystalline phase that melts just above room temperature,29 reinforcing the rubbery character of the material . the mobility of the mwcnts is enhanced in a more  fluid  polymer matrix , and this makes it easier for individual nanotubes to connect each other leading to the decrease of the resistance that had been observed within different measurement cycles.26 the relative importance of this contribution has been strongly reduced by the annealing treatment and paying the price of a reduced sensitivity to improve the stability of the films and to obtain more reproducible measurements . figure 3a shows the transmission electron microscopy ( tem ) image ( cm12 , philips ) of mwcnts  sebs after a sonication of 5 minutes . sebs is not visible during tem analysis ; however , figure 3a allows the good dispersion of mwcnts in sebs to be confirmed . figure 4 shows the calibration curve for an annealed nanocomposite mwcnts  sebs ( 20% w / w of mwcnts ) obtained over seven repeated measurement cycles , from minimum to maximum temperature and vice versa . figure 5 shows the calibration curves for different ph values at go concentrations of 0.04 , 0.4 , and 4 mg / ml . figure 3b shows the scanning electron microscope ( sem ) image ( xl30 , philips ) of a go film ( thickness ~90 nm ) for a concentration of 4 mg / ml . in a recent study,34 we reported as the sensor was able to record ph over 3 days in human plasma . the same sensor was used after 20 days from immersion in plasma for another 3-day measurement campaign and observed a difference of only 0.140.09 ph units compared with the commercial ph meter ( figure 6 ) . the temperature sensor showed a high linearity ( r2=0.999 ) and sensitivity ( ~85 /c ) between 25c and 50c . the annealing improved both the linearity and the repeatability , thus indicating a stabilization of the nanocomposite micro - domains . the size and shape of the kapton substrate can be adapted to the dimension of the foot ulcer cavity for a smooth insertion . before testing the sensor on patients the role of the coating is twofold : it serves as a protection layer to avoid the nanocomposite to be damaged and to guarantee the biocompatibility in contact with the ulcer . for this purpose , a layer of parylene c could be used because its biocompatibility is already established in the literature , although its effect on the shrinkage / expansion of the nanocomposite should be investigated.34,35 the ph sensor proved to be suitable for ph measurement during laboratory tests . when the concentration of go was increased up to 4 mg / ml , the sensitivity ( ~43 mv / ph ) , linearity ( r2=0.99 ) , and repeatability ( three measurements per ph value , 0.2 ph units ) improved . these results are sufficient for considering this sensor as a potential candidate for tests on patients . the go biocompatibility of this sensor has not yet been assessed , but a study suggested that at concentration < 20 g / ml , the go should not be cytotoxic.36 in this study , we described two temperature- and ph - sensitive materials that may be used to monitor chronic ulcers status over time . the kapton board is suitable to be used for tests with patients and part of future work will be dedicated to design a kapton board where both sensors can be fabricated . the tests performed in laboratory showed promising results , but we are aware that these sensors still need to be clinically validated ; however , some pivotal experiences with non - wearable temperature sensors on df patients in a real clinical setting have already demonstrated how profitable such an information could be in terms of a better understanding of the clinical course of the disease , that encompass both chronic sub - clinical phases and acute phases that are mostly triggered by super - infection of chronic ulcers and of improving the clinical outcomes because of the timely modifications of local and systemic therapies.1719 therefore , the possibility of introducing wearable sensors , eventually embedded in dressings or bandaging , for a real - time monitoring of variables such as ph and temperature in df - ulcerated patients could help clinicians in improving the quality of treatments for such a complex pathology , specially increasing the timeliness of intervention , that has been demonstrated to be directly correlated to its effectiveness , both in terms of reduction of leas and healing of the ulceration.3739 future work will be to perform a randomized controlled trial , including a sterilization process for the materials ; to confirm this hypothesis ; and to validate this new technology with clinical evidence .
foot ulcers account for 15% of comorbidities associated with diabetes . 
 presently , no device allows the status of foot ulcers to be continuously monitored when patients are not hospitalized . in this study 
 , we describe a temperature and a ph sensor capable of monitoring diabetic foot and venous leg ulcers developed in the frame of the seventh framework program european union project swan - icare ( smart wearable and autonomous negative pressure device for wound monitoring and therapy ) . 
 temperature is measured by exploiting the variations in the electrical resistance of a nanocomposite consisting of multiwalled carbon nanotubes and poly(styrene - b-(ethylene - co - butylene)-b - styrene ) . 
 the ph sensor used a graphene oxide ( go ) layer that changes its electrical potential when ph changes . 
 the temperature sensor has a sensitivity of ~85 /c in the range 25c50c and a high repeatability ( maximum standard deviation of 0.1% over seven repeated measurements ) . for a go concentration of 4 mg / ml 
 , the ph sensor has a sensitivity of ~42 mv / ph and high linearity ( r2=0.99 ) .