all cases of cryptosporidiosis in england and wales reported to national surveillance from 1989 through 2005 were analyzed ; those associated with recent foreign travel were excluded . the average weekly number of cryptosporidiosis cases preregulation ( 19891999 ) were plotted against the same data postregulation ( 20002005 ) ( figure ) . since the regulations were implemented , fewer cryptosporidiosis cases have occurred in the first half of the year but more in the second . however , as the standard deviation bars on the figure indicate , the number of cases fluctuated from year to year both before and after the regulations . this trend makes it difficult to ascertain whether the changes after regulation are part of the natural interannual variability or represent real changes in incidence . sd , standard deviation climatic variability and community spread from imported travel cases are suggested as the main sources of this interannual variability ( 4,5 ) . precipitation may wash cryptosporidium organisms from land into public water supplies , and warm , dry weather may increase the number of countryside visits . both of these could result in exposure to cryptosporidium organisms . consequently , we developed a predictive model of weekly cryptosporidiosis cases using weekly incidence data ( 19891999 ) and national data on temperature , rainfall , river discharges , and reported number of travel - associated cases . the results indicated that between mid - march and the end of june cryptosporidiosis cases were positively associated with river discharges that occurred 2 weeks previously . from july through early september , cryptosporidiosis was positively associated with warm , dry weather in the previous 2 months . comparable data on temperature , rainfall , and river discharges were obtained for the postregulation period ( 20002005 ) and entered into the predictive model . this estimated the number of cases that would have been expected , for each week , from 2000 through 2005 . to provide an overview of these predictions , the estimates were summed to produce totals for each half of the year , for every year after regulation . the results are presented in the table alongside the 95% confidence interval of the prediction , the actual numbers of cases reported , and the difference between the actual and predicted cases . in the first half of the year , cryptosporidiosis was significantly reduced ( p<0.05 ) every year since 2000 . for this finding to be attributable to the regulations , cryptosporidiosis has been associated with recreational swimming and person - to - person contact and , to our knowledge , the levels of these have remained unchanged . the greatest reduction in cases occurred in the first half of 2001 , a period that coincides with the foot - and - mouth disease epidemic . this epidemic led to the slaughter of > 6 million livestock and restricted public access to agricultural land ( 6 ) . the large reduction in cases in 2001 has been attributed to this epidemic ( 7,8 ) , but our results indicate that cases were already depressed in the first half of 2000 , and these reductions continued into 2002 . therefore , the large reduction observed in the first half of 2001 is also likely to be due to the new drinking water regulations . another reason for lower cryptosporidiosis incidence since 2000 could be lower levels of cryptosporidium spp . in livestock after the foot - and - mouth epidemic ( 9 ) . however , a recent study has discounted this ( 3 ) , and factors associated with the 2001 epidemic can not explain the reductions in cases observed in 2000 . we conclude , therefore , that improved water treatment associated with the new drinking water regulations has led to cryptosporidiosis reductions during the first half of the year . in the second half of the year , the numbers of cases are significantly ( p<0.05 ) lower than predicted in 2001 , 2002 , and 2004 , but significantly higher ( p<0.05 ) in 2000 and 2003 . one explanation for the excess cases in the second half of 2000 and 2003 is that they may represent unreported travel - associated cases or community transmission from these cases . the table demonstrates that many foreign travel  associated cases occurred in both these periods ( > 300 in 2000 and 2003 compared with < 200 for other years ) , and these are poorly recorded in national surveillance ( 10 ) . this inconsistency in the pattern between years , combined with the potential link between excess cases and travel - associated cases , led us to conclude that the overall increase in incidence in the second half of the year is not likely to be related to the regulations . by averaging the differences between the observed and predicted cryptosporidiosis cases across the years , we can estimate the public health benefits of the regulations . the average excludes 2001 because of the confounding effect of the foot - and - mouth epidemic . since 2000 , an annual average reduction of 615 reported cases has occurred . this reduction comprises a large decrease in the first half of the year and a small increase in the second half . if we assume that the increase in cases in the second half of the year is not associated with drinking water , the benefit of the intervention is 905 reported cases per year ( the average reduction in the first half of the year ) . not all cases of cryptosporidiosis in the community are reported to national surveillance , and the ratio of reported to community cases is estimated to be 7.4 ( 11 ) . if this multiplier is applied to our estimate of 905 cases , it implies 6,770 fewer cases of cryptosporidiosis in the community each year . two recent reports have suggested that even this multiplier may be an underestimate ( 12,13 ) . we have presented evidence that new drinking water regulations implemented in england and wales during 2000 led to significantly fewer cryptosporidiosis cases in the first half of the year with no significant change in the second half of the year . we estimate a reduction in reported cases of 905 per year or 6,770 cases in the community each year . these findings indicate that regulations such as those implemented in england and wales can have a significant public health benefit in reducing cases of cryptosporidiosis .
since new drinking water regulations were implemented in england and wales in 2000 , cryptosporidiosis has been significantly reduced in the first half of the year but not in the second . 
 we estimate an annual reduction in disease of 905 reported cases and 6,700 total cases .