environmental pollution and occupational exposure to heavy metals are considered possible cofactors in the occurrence of both chronic and malignant diseases . the bulk of epidemiologic and in vitro studies , as well as studies in experimental animals , support the hypothesis that a cause and effect relationship exists between chronic irritation ( oxidative stress ) by heavy metals and carcinogenesis . unfortunately , there is a complete lack of relevant human studies , in particular concerning pathologic examination of serial samples collected at various time intervals and showing host hodgkin s disease is a malignant systemic lymphoma which affects a large number of young people . its occurrence has been occasionally related to viral pathogenesis because of the prevalence of epstein  barr virus infection in different types and locations of lymphoma.14 sometimes the clinical history starts with remitting episodes of fever followed by lymph node swelling , biopsy and pathologic examinations , which detects typical malignant degeneration , with reed the progression from nonspecific lymphadenitis to lymphoma is seldom histologically depicted because of the speed of transformation . our case report describes a 25-year - old man who , at the beginning of his disease , had lymph node enlargement for at least one year and underwent repeated biopsy of the nodes and bone marrow until a diagnosis of hodgkin s lymphoma was finally made . we have investigated the possibility of a link between exposure to environmental particulate matter and development of lymphoma5,6 using a new investigational tool , ie , field emission gun - environmental scanning electron microscopy ( feg - esem ) coupled with energy dispersive spectroscopy ( eds ) to determine the chemical composition of the particles identified . this research has highlighted the presence of heavy metal nanoparticles in cells and opens the debate on their pathogenetic role in the onset of hodgkin s disease . he was a racing car driver who was regularly exposed to toxic air pollution . in 1985 , the patient underwent appendectomy for acute appendicitis with moderate leukocytosis ( white blood cells).4,10 two years later , he suffered from head and spine trauma , followed by a motorbike accident the following year . the latter led to a fracture of the left tibia which was surgically treated with a titanium pin . in may 1993 , the patient was hospitalized due to an unremitting fever ( 37.5c ) which lasted for one month . a laboratory analysis was performed , and the results were negative for cytomegalovirus , pathogenic fecal bacteria , hic , human immunodeficiency virus , epstein barr virus , brucella , shigella , and salmonella typhimurium , but a toxoplasmosis test was positive , and a computed tomography scan showed enlarged paraortic lymph nodes and an enlarged left inguinal lymph node . a needle biopsy of the periaortic lymph nodes and a bone marrow biopsy showed an inflammatory mixed cell response . in july 1993 , the surgical biopsy of a left inguinal node led to the diagnosis of reactive lymphadenitis with widespread immature sinus histiocytosis , but without paracortical tissue enlargement and a normal immunophenotype . in february 1994 , five months later , the radiogallium lymph node scintigraphy suggested a possible diagnosis of sarcoidosis . two months later , the patient suffered a sports - related thoracic trauma which resulted in an emergency hospital admission . a plain chest x - ray showed bilateral hilar polycyclic lymphadenopathy with right upper and middle lobe infiltration , raising the suspicion of lymphoma . a bone marrow biopsy showed a pattern related to chronic infections and atypical lymphocytes ( less than 5% ) , whereas a right inguinal lymph node biopsy showed weakly positive leu m1 reed lymphatic cells were uchl1- and cd3-positive ( t lymphocytes ) , and a minority were l26-positive ( b lymphocytes ) . finally , hodgkin s lymphoma stage ivb ( scleronodular mixed cell subtype ) was diagnosed . the patient underwent radiochemotherapy with partial volumetric regression of the right thoracic paraortic lymph nodes . in 1997 , further lesions were found in the spleen . the total body positron emission tomography revealed the involvement of the liver at the vii segment level , confirmed by an ultrasound scan showing additional lesions at the v , vii , and viii segments . a further lymph node biopsy confirmed the presence of hodgkin s disease ( scleronodular mixed cell subtype ) . tissue samples analyzed included a left inguinal lymph node in 1993 , a right lymph node in the groin area in 1994 , a bone marrow biopsy in 1994 , and another lymph node in 1998 . biopsy samples of benign ( on the right ) and malignant ( on the left ) lymph nodes and bone marrow were fixed in 10% formalin , dehydrated in ascending concentration alcohols , and embedded in paraffin . a leica microtome ( rm 2125rt ; lecia nussloch , germany ) after that , with a few drops of xylol and 98% alcohol , they were mounted on an aluminum stub and inserted inside the chamber of a feg - esem quanta ( fei company , eindhoven , the netherlands ) coupled with an eds ( edax , mahwah , nj ) . the use of the feg - esem was recommended because it does not need any further process to make the sample electron - conductive ( gold or carbon coating of the biologic sections ) . the eds microanalysis system was used to identify the elemental composition of inorganic materials ( foreign bodies ) identified in the biopsy samples . the working conditions of the feg - esem were low - vacuum , from 20 to 30 kv , in secondary and backscattered electron modality , with spots 35 . for every sample , a list of the particles found in the sample was given , along with their size , morphology , and chemical composition . table 1 shows a summary of the most representative particles identified in the lymph node and bone marrow biopsies , taken at different stages of the disease , described according to morphology , size and chemical composition . particle accumulation is not uniform , and this might have been due to asymmetry of the lymphatic circulation . these are not biocompatible and , over time , release highly toxic ions , including iron , chromium , and nickel . our investigation shows an increased amount of metallic foreign bodies , with progression of the disease and progressive amounts of submicroscopic calcium storage.7 chromium compounds produce a variety of types of dna damage , including single - strand breaks , alkali - labile sites , and dna protein crosslinks . these compounds bind isolated dna and proteins , readily enter cells via anion channels , but do not cross cell membranes . chromium inhibits the transcriptional activity of nuclear factor - kappa b by decreasing the interaction of p65 with camp - responsive element - binding protein.8,9 figure 1 shows submicronic particles ( white spots ) in the left lymph node biopsy sample . these particles appear whiter than the biologic substrate because they are atomically denser . the biologic tissue is only composed of carbon , oxygen , phosphorus , sulphur , and chlorine , whereas the particles contain chromium with traces of calcium . chromium is chemically toxic in biologic tissue and can also release chromium ions which worsen the toxicity.1013 raised concentrations of circulating metal degradation products , derived from orthopedic implants , may have deleterious biologic effects over time . accurate monitoring of metal concentrations in serum and urine , after total hip replacement , can provide insights into the mechanisms of metal release . corrosion significantly raises metal levels , including nickel and chromium in serum and urine , when compared with patients with no radiologic evidence of corrosion and volunteers without metallic implants ( p < 0.001).1418 samples of the progression of the disease are shown in figures 2 and 3 . our case report describes for the first time a pathophysiologic and ultrastructural five - year follow - up of lymph nodes obtained from the same patient . it shows the progressive storage of heavy metal nanoparticles in lymphatic tissue , which was in parallel with progression of a cancerous process from early chronic inflammation to hodgkin s disease . this is a unique case report relating to one - year progression from lymphadenitis to lymphoma in the inguinal region of a patient , where the increasing presence of foreign inorganic microsized and nanosized bodies has been demonstrated . although the patient underwent appendectomy and tested positive for toxoplasmosis which may be associated with malignancies , this relationship was unlikely in our patient . his appendectomy dated back to childhood , and toxoplasmosis did not lead to infection with encephalic damage . heavy metals , such as iron , chromium and nickel , are the main constituents of foreign bodies found in cells.9,11,1921 occupational or incidental heavy exposure to many chemicals , including benzene , pesticides , ethylene oxide and metals ( mercury , cadmium , chrome , cobalt , lead , aluminum ) is very dangerous for the hematopoietic system . mercury and chromium cause immunosuppression and evoke autoimmune reactions.1012,2225 an epidemiologic study assessing cancer risk in a small population exposed to excessive amounts of chromium9 reported two cases of hodgkin s disease after protracted exposure and a long latency period . the calculated and observed risk in this study is 6592-fold higher than in nonexposed populations . clinicians should be aware of the potential association between hexavalent chromium exposure and hodgkin s lymphoma . their identification in anatomic areas , far from the putative route of entry , raises the issues of whether the mechanism of entrance , internal distribution ( streaming ) , and uptake ( selective or not ) in the human body involves a potential respiratory or digestive source , and the potential pathogenic mechanism by which nonbiocompatible nanosized foreign bodies cause a chronic inflammatory reaction in lymphatic tissue progressing to hodgkin s lymphoma.11,19,21,26 the possibility of environmental pollutants spreading inside the body after inhalation has been shown by pry et al27 in a study involving five volunteers after inhalation of technetium - radiolabeled 100 nm carbon particles . they demonstrated that these radiolabeled nanoparticles overwhelm the lung barrier within 60 seconds and are stored in the liver within 60 minutes . after this time , the radioactive signal decreases , but there can be a wider dispersion through the blood circulation . evidence of submicronic particles spreading in different organs or regions , including sperm and brain tissue , has been shown previously . the transport of nanosized particles , due to their consistently small size , may be mediated by blood cells or other blood or lymph components . in particular , metal nanoparticles may produce toxic ions locally by delayed corrosion mechanisms.1418 trapping of these nanoparticles within lymphatic cells , with mutagenic and carcinogenic potential via an inflammatory reaction , could elicit a cytokine cascade and lymph node engulfment , with further critical concentration of nanomaterials up to the level inducing carcinogenic mutations . with regard to the present case report , it would have been helpful if the patient had been followed up during the five years preceding 1993 , ie , when the progression from chronic inflammation to cancerous disease actually occurred . however , it is important to bear in mind the difference between experimental research using animal models and that using human subjects , whereby the latter must conform to more stringent ethical rules . in the absence of more robust data on humans demonstrating a clear - cut cause and effect relationship between chronic exposure to heavy metals and malignancies ( not only on the basis of epidemiologic correlation studies , but on the basis of pathologic evidence , such as in the present report ) , however , caution is recommended when interpreting the results of a single case report , as well as those of small studies such as that one by bick et al,11 in which small numbers could be responsible for bias in risk calculation . in addition , as our extended studies have already suggested,5,6,2833 environmental pollutants are usually present in such a small concentration in the atmosphere that they are unlikely to be solely responsible for the occurrence of malignant disease in the absence of a congenital or inherited predisposition in the host . on the contrary , in the presence of frail predisposed subjects , even small amounts of pollutants could be cofactors for the occurrence of chronic or even malignant diseases.1 our anecdotal report highlights the need for ultrastructural investigation in a larger sample of patients with hodgkin s lymphoma . it suggests the possibility that , at least in some subtypes , or in a proportion of subjects with hodgkin s disease , the progression from chronic lymphadenitis to overt malignancy can be facilitated or codetermined in addition to genetic or congenital factors . moreover , it is likely to happen by chronic exposure or accumulation of heavy metal nanoparticles within lymph nodes , which could determine additional carcinogenetic mutations .
a 25-year - old man had complained of sudden fever spikes for two years and his blood tests were within the normal range . in 1993 , 
 a surgical biopsy of swollen left inguinal lymph nodes was negative for malignancy , but showed reactive lymphadenitis and widespread sinus histiocytosis . 
 a concomitant needle biopsy of the periaortic lymph nodes and a bone marrow aspirate were also negative . in 1994 , after an emergency hospital admission because of a sport - related thoracic trauma , a right inguinal lymph node biopsy demonstrated hodgkin s lymphoma stage ivb ( scleronodular mixed cell subtype ) . 
 although it was improved by chemotherapy , the disease suddenly relapsed , and a further lymph node biopsy was performed in 1998 confirming the same diagnosis . despite further treatment , the patient died of septic shock in 2004 , at the age of 38 years . 
 retrospective analysis of the various specimens showed intracellular heavy metal nanoparticles within lymph node , bone marrow , and liver samples by field emission gun environmental scanning electron microscopy and energy dispersive spectroscopy . 
 heavy metals from environmental pollution may accumulate in sites far from the entry route and , in genetically conditioned individuals with tissue specificity , may act as cofactors for chronic inflammation or even malignant transformation . 
 the present anecdotal report highlights the need for further pathologic ultrastructural investigations using serial samples and the possible role of intracellular nanoparticles in human disease .