vibrio phage valkk3 was isolated from marine sediment of kota kinabalu , malaysia that was capable to lyse fish and human pathogen , vibrio alginolyticus atcc 17749 . the genome was extracted and purified using dneasy blood and tissue kit ( qiagen ) . the paired of 250  bp reads ( 2    250,000 samples ) were generated and demultiplexed corresponding to the barcodes . the sequence assembly was performed using de novo assembly using velvet assembly software version 1.1 ( zerbino , european bioinformatics institute , uk ) . the assembly producing the lowest number of contigs with the largest n50 value was chosen for further assembly with a  >  30-fold sequence coverage into single contig . the complete contig sequence was subjected to blastn   and the sequence was annotated using blast2go . 1 ) showed that it belongs to the family of myoviridae . to the best of our knowledge , among the giant t4-like phages reported so far ( table 1 ) , the vibrio phage valkk3 is the only strain that can infect v. alginolyticus . the genome annotation analysis predicted 390 open reading frames which are numbered consecutively from orf1 to orf390 which represented 92.4% of the total genome . the putative genes varied from 111  bp ( orf268 ) to 4200  bp ( orf349 ) . the protein function analysis showed that some cdss involve in repression of host gene synthesis ( orf197 , orf202 ) , defense system of own gene synthesis ( orf170 , orf176 , orf207 ) and quality control ( orf255 ) , dna synthesis ( orf61 , orf199 , orf367 , orf370 , orf241 , orf256 , orf381 ) , dna replication and recombination ( orf371 , orf372 , orf320 , orf178 , orf204 , orf106 , orf242 , orf257 ) , structural protein : the head ( orf281286 ) , tail ( orf287319 ) and tail fiber ( orf346349 ) , dna packaging protein ( orf289 , orf290 ) and host lysis protein ( orf304 ) . in this study , the putative functions of valkk3 genome only represent 15.6% of the genes in the valkk3 . the other 84.4% in the valkk3 are consisted of the genes which encoded proteins with unknown function or hypothetical proteins . the valkk3 is similar to the vibrio phage vh7d , bacteriophage kvp40 , vibrio phage phi - pp2 and vibrio phage nt-1 ( table 1 ) . this assigns the valkk3 to the t4-like phage genus of the myoviridae family which belongs to the giant vibriophage group ,   with even larger genome size . the blast search revealed that valkk3 was homologous to vibriophage that is known to infect some members of harveyi clade bacteria . it is also revealed that t4-like phage is widely spread in the marine environment infecting different vibrio species . finally , an additional genome information of valkk3 is hoped to provide positive contributions to the study of vibrio bacteriophage . this complete genome sequence has been deposited at ddbj / embl / genbank under accession no . kp671755 .
this paper describes the complete sequence of a giant lytic marine myophage , vibrio phage valkk3 that is specific to vibrio alginolyticus atcc 17749. vibrio phage valkk3 was subjected to whole genome sequencing on miseq sequencing platform and annotated using blast2go . 
 the complete sequence of valkk3 genome was deposited in dbbj / embl / genbank under accession number kp671755 .