this study was part of a large multinational trial comparing artesunate versus quinine in the treatment of severe falciparum malaria in african children ( aquamat , registration number isrctn50258054 ) , the results of which have been published elsewhere . this substudy was conducted at hospital central da beira ( hcb ) , beira , mozambique , between october 2005 and july 2010 . hcb is an 800-bed tertiary referral hospital in the port city of beira , which is built along the swampy grounds at the mouth of the pungwe river where malaria transmission is perennial and malaria is mesoendemic . the hiv burden in the city of beira is reflected in the hiv prevalence in pregnant women , reported as approximately 30% in 2004 . ethics approval , including confidential hiv and cd4 testing , was granted by the comit nacional de biotica para a sade in mozambique and the oxford tropical research ethics committee . children ( < 15 years ) and adults ( 15 years ) presenting with suspected severe malaria according to modified world health organization ( who ) clinical criteria were screened using a plasmodium lactate dehydrogenase ( pldh)based and plasmodium falciparum histidine - rich protein-2 ( pfhrp2)based rapid diagnostic test ( rdt ) and a peripheral blood slide [ 22 ,  23 ] . patients with a positive pldh - based rdt were included in the trial , provided that full written informed consent was given by the patient or carer . severity criteria included decreased consciousness ( coma or severe prostration ) , convulsions , respiratory distress or acidotic breathing , shock , severe symptomatic anemia ( < 5  g / dl ) , hypoglycemia ( <3 mmol / l ) , hemoglobinuria , severe jaundice , or a convincing history of anuria or oliguria in adult patients . a venous blood sample was taken for peripheral blood parasite counts , hematocrit ( hct ) , quantitative assessment of plasma pfhrp2 ( a marker of total body parasite burden ) [ 24 ,  25 ] , biochemistry and acid  base parameters ( ec8 cartridge for i - stat handheld analyzer ) , as well as hiv testing and cd4 lymphocyte count . hiv antibody testing was performed according to the sequential test algorithm by the mozambican hiv testing guidelines , comprising of a screening test ( determine hiv-1/2 , abbott laboratories ,  abbott park , il ) followed by a confirmation test ( uni - gold hiv , trinity biotech plc , bray , ireland ) in case of a positive screening result . clinical staff and patients did not have access to the hiv-1 test results and laboratory staff did not have access to patient data . surviving patients or their guardians received voluntary counseling and testing after recovery , and if indicated , were offered further treatment and follow - up according to national guidelines . quality assessment of slide reading was performed at the mahidol - oxford tropical medicine research unit in bangkok on 1348/2017 ( 67% ) of screening slides and 100% of slides of enrolled patients , and was used as reference . quantitative parasite counts were calculated from thin film ( count per l =  count per 1000 red blood cells    125.6    hct ) assuming an mcv of 80 fl   or thick film ( count per l  =  count per 200 white blood cells [ wbc ] x 40 ) assuming a wbc of 8000/l [ 26 ,  27 ] . data were analyzed with stata , version 12 ( statacorp , college station , tx ) . categorical variables were compared between hiv - negative and hiv - positive cases with  or fisher 's exact test . normally distributed or log - normalized variables were compared using a student t test , the remainder using mann weight - for - age z scores as a measure of malnutrition were calculated using stata applications based on the who child growth standards [ 28 ,  29 ] . strictly  defined severe malaria was based on modified who criteria [ 19 ,  22 ] . in hiv - positive patients , cd4 lymphocyte count or percentage - based immunological staging ( not significant , mild , advanced , severe ) was performed according to the who classification . to determine the prognostic significance of hiv coinfection or the who hiv immunological stage classification , a logistic regression model was constructed with death as the dependant variable and hiv infection in addition to established predictors of death as the independent variables , including coma , prostration , convulsions , hypoglycemia , respiratory distress , shock , hemoglobin ( g / dl ) , base excess ( be ; mmol / l ) , log blood urea nitrogen ( mg / dl ) , log parasitemia ( parasites/l ) , plasma pfhrp2 [ as log and ( log ) , ng / ml ] [ 19 ,  24 ,  25 ,  3133 ] and weight - for - age z scores . since the case fatality rates between children and adults differed significantly ( p  <  .0001 ) and the number of adult participants was limited , only children were included in the logistic regression model , which was adjusted for age and antimalarial treatment ( artesunate or quinine ) [ 19 ,  35 ] . using a stepwise approach , only covariates that were significant at p  <  .05 were retained in the final model . data were analyzed with stata , version 12 ( statacorp , college station , tx ) . categorical variables were compared between hiv - negative and hiv - positive cases with  or fisher 's exact test . normally distributed or log - normalized variables were compared using a student t test , the remainder using mann weight - for - age z scores as a measure of malnutrition were calculated using stata applications based on the who child growth standards [ 28 ,  29 ] . strictly  defined severe malaria was based on modified who criteria [ 19 ,  22 ] . in hiv - positive patients , cd4 lymphocyte count or percentage - based immunological staging ( not significant , mild , advanced , severe ) was performed according to the who classification . to determine the prognostic significance of hiv coinfection or the who hiv immunological stage classification , a logistic regression model was constructed with death as the dependant variable and hiv infection in addition to established predictors of death as the independent variables , including coma , prostration , convulsions , hypoglycemia , respiratory distress , shock , hemoglobin ( g / dl ) , base excess ( be ; mmol / l ) , log blood urea nitrogen ( mg / dl ) , log parasitemia ( parasites/l ) , plasma pfhrp2 [ as log and ( log ) , ng / ml ] [ 19 ,  24 ,  25 ,  3133 ] and weight - for - age z scores . since the case fatality rates between children and adults differed significantly ( p  <  .0001 ) and the number of adult participants was limited , only children were included in the logistic regression model , which was adjusted for age and antimalarial treatment ( artesunate or quinine ) [ 19 ,  35 ] . using a stepwise approach , only covariates that were significant at p  <  .05 were retained in the final model . between october 2005 and july 2010 , 896 adults were screened for severe malaria , out of whom 87 ( 9.7% ) had a positive pldh - based rdt , an inclusion criterion for the treatment trial ( figure  1 ) . out of these , 68 adults were enrolled in the treatment trial . in 1272 children with suspected severe malaria , 731 or 57.5% ( 724 positive pldh and pfhrp2 tests , 7 with positive pfhrp2-based test only ) had a positive rdt of which 655 were enrolled . in screened adult patients with quality assessment of the peripheral blood slide ( n  =  513 ) , the sensitivity of the pfhrp2-based rdt compared to expert microscopy was 44.3% and the specificity was 95.2% . for the pldh - based rdt , sensitivity was 38.8% and specificity 98.5% . in children ( n  =  835 ) , the sensitivities of these rdts were 92.6% and 85.3% , and the specificities were 64.5% and 83.3% , respectively . the combination of a negative rdt and a positive malaria slide was associated with low parasitemia . the parasite density ( geometric mean ; 95% confidence interval [ ci ] ) with a negative pfhrp2-based test and positive slide with reported parasite density was 232 ( 141383 ) parasites/l in adults ( n  =  89 ) and 180 ( 90359 ) parasites/l in children ( n  =  40 ; p  =  .56 ) . figure  1.study profile.abbreviations : hiv , human immunodeficiency virus ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 ; pldh , plasmodium lactate dehydrogenase ; rdt , rapid diagnostic test . patients with discordant hiv antibody test results ( n  =  4 ) were excluded from the analysis . four children aged < 18 months were classified as hiv positive on the basis of their serostatus in absence of viral load testing and were included . seroprevalence was 74/655 ( 11% ) in children and 49/68 ( 72% ) in adults . hiv - positive status in coinfected patients was reported on admission in 9/74 ( 12% ) of children and 5/49 ( 10% ) of adults , of which 5 and 4 were on antiretroviral treatment , respectively . by history of the carer , 10/74 ( 14% ) of hiv - coinfected children had a prior chronic illness ( 6 tuberculosis , 2 tuberculosis with chronic otitis media , 2 chronic otitis media ) , versus 20/579 ( 3% ) of hiv - uninfected children ( 8 tuberculosis , 1 sequelae of previous cerebral malaria , 4 congenital heart disease , 1 chronic otitis media , 6 bronchial asthma , 2 no information ; p  < .001 ) . in hiv - coinfected adults , 11/49 ( 22% ) had a history of chronic disease ( 5 tuberculosis , 1 hemiparesis after stroke , 1 epilepsy , 1 hepatitis , 3 herpes zoster ) versus 1/16 ( 6% ) ( hypertension , 3 no information ; p  =  .108 ) . table  1.baseline characteristics of children and adults with severe malaria according to hiv statuscharacteristicchildren < 15  yearsadults 15  yearshiv negative ( n  =  581)hiv positive ( n  =  74)p valuehiv negative ( n  =  19)hiv positive ( n  =  49)p valuefemale279 ( 48%)33 ( 45%).587 ( 37%)22 ( 45%).55age , years ( median , iqr)3.6 ( 2.55.0)5.0 ( 3.08.0).000118 ( 1623)27 2538.0002presenting symptoms  coma441 ( 76%)54 ( 73%).5813 ( 68%)35 ( 71%).81  convulsions506 ( 87%)56 ( 76%).00810 ( 53%)18 ( 37%).23  prostration132 ( 23%)19 ( 26%).575 ( 26%)12 ( 24%)1.0  shock21 ( 4%)3 ( 4%).740 ( 0%)3 ( 6%).55  severe respiratory distress37 ( 6%)14 ( 19%)<.0011 ( 5%)6 ( 12%).66severe acidosis ( be 8  mmol / l)108 ( 22%)26 ( 38%).0041 ( 8%)12 ( 27%).26hypoglycemia32 ( 6%)8 ( 11%).071 ( 5%)2 ( 4%)1.0severe anemia with respiratory distress67 ( 12%)15 ( 21%).0280 ( 0%)3 ( 6%).55black water fever24 ( 4%)5 ( 7%).364 ( 21%)10 ( 20%)1.0severe jaundice15 ( 3%)5 ( 7%).062 ( 11%)4 ( 8%)1.0anuria / oliguria    0 ( 0%)2 ( 4%)1.0hyperparasitemia ( > 10%)106 ( 21%)21 ( 32%).0433 ( 21%)12 ( 29%).74strictly defined  severe malaria533 ( 94%)66 ( 89%).0816 ( 94%)41 ( 89%)1.0abbreviations : be , base excess ; hiv , human immunodeficiency virus ; iqr , interquartile range . for children : compensated and decompensated shock combined . in adults : only decompensated shock . in adults only . based on who criteria . baseline characteristics of children and adults with severe malaria according to hiv status abbreviations : be , base excess ; hiv , human immunodeficiency virus ; iqr , interquartile range .   for children : compensated and decompensated shock combined . expert slide readings with quantitative results were available from 575/655 ( 88% ) of enrolled children with known hiv status . geometric mean ( 95% ci ) parasitemia was 47  140 ( 37  98858  498 ) in hiv - negative ( n  =  510 ) and 67  977 ( 37  143124  408 ) in hiv - positive children ( geometric mean ( 95% ci ) plasma pfhrp2 concentration assessed in 653/655 ( > 99% ) children was 831 ( 707975 )  ng / ml in hiv - negative ( n  =  510 ) and 1395 ( 9112136 )  ng / ml in coinfected children ( n  =  65 ; p  =  .0321 ; figure  2 ) . figure  2.comparison of the circulating peripheral blood parasitemia ( left y - axis ) and plasma pfhrp2 concentration as a measure of the total body parasite burden ( right y - axis ) , between human immunodeficiency virus ( hiv)negative (  , n  =  510 ) and hiv - positive (  , n  =  65 ) children with quantified plasma pfhrp2 and peripheral blood parasitemia . abbreviations : ci , confidence interval ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . comparison of the circulating peripheral blood parasitemia ( left y - axis ) and plasma pfhrp2 concentration as a measure of the total body parasite burden ( right y - axis ) , between human immunodeficiency virus ( hiv)negative (  , n  =  510 ) and hiv - positive (  , n  =  65 ) children with quantified plasma pfhrp2 and peripheral blood parasitemia . abbreviations : ci , confidence interval ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . hiv - coinfected children were older and differed in their clinical presentation from uninfected children with an increased frequency of severe acidosis and severe anemia ( both clinically and laboratory assessed ) and respiratory distress ( tables  1 and 2 ) . blood urea nitrogen ( bun ) concentrations were also higher in hiv - infected children . on physical examination , hiv - positive children had significantly lower weight - for - age z scores and more frequent oral candidiasis and lymphadenopathy . in the adult patients , table  2.clinical examination and laboratory assessments in patients with severe malaria according to hiv statusassessmentchildren < 15  yearsadults 15  yearshiv negative ( n  =  581)hiv positive ( n  =  74)p valuehiv negative ( n  =  19)hiv positive ( n  =  49)p valueclinical examinationweight - for - age z score1.0 ( 1.3)1.6 ( 1.1).0001    temperature ( c)38.2 ( 0.9)38.3 ( 1.0).7638.1 ( 1.2)38.2 ( 1.3).76blood pressure ( mmhg )  systolic103 ( 16)104 ( 16).48112 ( 16)113 ( 22).82  diastolic64 ( 13)65 ( 16).6569 ( 14)69 ( 13).89respiratory rate ( breaths / min)39 ( 10)39 ( 9).9826 ( 5)29 ( 8).14coma depth ( n , median , range )  bcs70 , 2 ( 05)7 , 2 ( 23).29 gcs511 , 8 ( 315)67 , 9 ( 315).28718,10 ( 314)48 , 8 ( 315).54oral candidiasis1 ( < 1%)9 ( 12%)<.00104 ( 9%).57lymphadenopathy16 ( 3%)20 ( 27%)<.0011 ( 6%)5 ( 10%).54laboratory assessments  bun ( mg / dl)12 ( 1112)16 ( 1319)<.000123 ( 1535)32 ( 2540).18  hemoglobin ( g / dl)8.2 ( 2.7)7.5 ( 2.7).03311.3 ( 2.6)10.2 ( 3.2).16  ph7.40 ( 0.11)7.38 ( 0.14).267.39 ( 0.09)7.37 ( 0.13).64  hco3 ( mmol / l)19.9 ( 4.9)17.7 ( 6.6).026721.8 ( 3.1)19.7 ( 5.7).17  base excess ( mmol / l)5 ( 6)7 ( 8).04613 ( 4)6 ( 8).43  slide pf positive547 ( 99%)72 ( 99%)1.018 ( 100%)45 ( 96%)1.0parasitemia ( parasites/l ) geometric mean ( 95% ci)47  141 ( 38  00558  474)68  320 ( 37  680123  874).25133  653 ( 59  082302  343)61  525 ( 24  628153  704).36pfhrp2 ( ng / ml ) geometric mean ( 95% ci)834 ( 712977)1452 ( 9832145).0197457 ( 932236)2471 ( 15094047).0072cd4 percentage  21 ( 1227)  13 ( 626)  cd4 absolute cell count  235 ( 118429)  199 ( 76405)  hiv - associated immunodeficiency  not significant  16 ( 23%)  6 ( 14% )  mild  13 ( 19%)  7 ( 16% )  advanced  12 ( 18%)  8 ( 19% )  severe  27 ( 40%)  22 ( 51%)data are no . ( % ) , mean ( sd ) , or median ( iqr ) , unless otherwise indicated.abbreviations : bcs , blantyre coma score ; bun , blood urea nitrogen ; ci , confidence interval ; gcs , glasgow coma score ; hiv , human immunodeficiency virus ; iqr , interquartile range ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . for children 10 years ( hiv - negative children , n  =  563 ; hiv - positive children , n  =  67 ) . bun was available in n  =  487 and n  =  67 children , n  =  15 and n  =  45 adults , respectively . hemoglobin was available in n  =  551 and n  =  70 children , n  =  19 and n  =  48 adults , respectively . ph was available in n  =  489 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . hco3 was available in n  =  490 and n  =  68 children , n  =  13 and n  =  44 adults , respectively . be was available in n  =  488 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . parasitemia reported from all patients with peripheral blood slide count , n  =  511 and n  =  66 children , n  =  14 and n  =  44 adults , respectively . pfhrp2 reported for all patients with pfhrp2 sample n  =  580 and n  =  73 children , n  =  18 and n  =  48 adults , respectively . cd4 percentage reported for hiv - infected children < 5 years ( n  =  34 ) and adults ( n  =  42 ) . cd4 absolute cell counts reported for hiv - infected children 5 years ( n  =  34 ) and adults ( n  =  43 ) . clinical examination and laboratory assessments in patients with severe malaria according to hiv status data are no . ( % ) , mean ( sd ) , or median ( iqr ) , unless otherwise indicated . abbreviations : bcs , blantyre coma score ; bun , blood urea nitrogen ; ci , confidence interval ; gcs , glasgow coma score ; hiv , human immunodeficiency virus ; iqr , interquartile range ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 .   for children 10 years ( hiv - negative children , n  =  563 ; hiv - positive children , n  =  67 ) . bun was available in n  =  487 and n  =  67 children , n  =  15 and n  =  45 adults , respectively . hemoglobin was available in n  =  551 and n  =  70 children , n  =  19 and n  =  48 adults , respectively . ph was available in n  =  489 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . hco3 was available in n  =  490 and n  =  68 children , n  =  13 and n  =  44 adults , respectively . be was available in n  =  488 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . parasitemia reported from all patients with peripheral blood slide count , n  =  511 and n  =  66 children , n  =  14 and n  =  44 adults , respectively . pfhrp2 reported for all patients with pfhrp2 sample n  =  580 and n  =  73 children , n  =  18 and n  =  48 adults , respectively .   cd4 percentage reported for hiv - infected children < 5 years ( n  =  34 ) and adults ( n  =  42 ) . cd4 absolute cell counts reported for hiv - infected children 5 years ( n  =  34 ) and adults ( n  =  43 ) . cd4 percentages and/or absolute counts were available for 68/74 ( 92% ) of hiv - positive children . increasing hiv - associated immunodeficiency according to the who classification was associated with increasing plasma pfhrp2 concentration ( nonparametric test for trend ; p  =  .022 ) but not with mortality ( p  =  .23 ) . comorbidities during hospitalization were more common in hiv - coinfected children ( 15/74 [ 20% ] ) compared with hiv - negative children ( 51/581 [ 9% ] ; p  =  .002 ) . pneumonia was suspected clinically in 19 ( 3% ) of hiv - negative children versus 9 ( 12% ) in hiv - positive children ( p  <  .001 ) . chest x - rays were sparsely available , and only a minority of cases had a radiologically confirmed diagnosis of pneumonia . although culture facilities were lacking , clinical sepsis was more common in hiv - coinfected children as well as a variety of other comorbidities ( table  3 ) . after admission , hiv - coinfected children developed more severe malaria related complications compared to hiv - negative children ( table  3 ) . hiv - coinfected children received more blood transfusions ( 38/74 [ 51% ] vs 224/581 [ 39% ] ; p =  .034 ) and antibiotics ( 65/74 [ 88% ] vs 447/581 [ 77% ] ; p  =  .033 ) in hiv - negative children . table  3.comorbidity , complications , and outcome according to hiv statuschildren < 15  yearsadults 15  yearshiv negative ( n  =  581)hiv positive ( n  =  74)p valuehiv negative ( n  =  19)hiv positive ( n  =  49)p valuecomorbidity  suspected pneumonia19 ( 3%)9 ( 12%)<.0011 ( 5%)2 ( 4%)1.0  confirmed by cxr5 ( 26%)4 ( 44%).411 ( 100%)0 ( 0%).3  clinical sepsis9 ( 2%)4 ( 5%).0490 ( 0%)1 ( 2%)1.0  suspected meningitis3 ( < 1%)1 ( 1%).381 ( 5%)01.0  gastroenteritis7 ( 1%)2 ( 3%).2700   other significant comorbidities22 ( 4%)7 ( 9%).02500  complications ( not present on admission )  development of coma3 ( < 1%)2 ( 3%).1011 ( 5%)2 ( 4%)1.0  deterioration coma score22 ( 4%)9 ( 12%).0012 ( 11%)6 ( 12%)1.0  convulsions developing or persisting > 6 hours after admission61 ( 11%)18 ( 24%).0012 ( 11%)3 ( 6%).61  respiratory distress6 ( 1%)7 ( 9%)<.0014 ( 21%)5 ( 10%).25  severe anemia ( < 5  g / dl)12 ( 2%)2 ( 3%).6701 ( 2%)1.0  black water fever13 ( 2%)4 ( 5%).1102 ( 4%)1.0  renal failure3 ( < 1%)2 ( 3%).103 ( 16%)8 ( 16%)1.0outcome  mortality53 ( 9%)19 ( 26%)<.0014 ( 21%)17 ( 35%).38  mortality in  strictly  defined severe malaria53/533 ( 10%)19/66 ( 29%)<.0014/16 ( 25%)17/41 ( 41%).36  neurological sequelae at 28  days6 ( 1%)2 ( 3%).2300  abbreviations : cxr , chest x - ray ; hiv , human immunodeficiency virus . no culture facilities available . defined as > 6 loose stools/24 h. other significant comorbidities in hiv - negative children included : suspected intoxication with traditional medicine , suspected hepatitis , burn of hand ( 1% ) , asthma / bronchitis , undefined skin rash , reactive arthritis , parasitosis ( tungiasis , ascaris ) , herpes simplex virus labial ulcera , acute otitis media , tonsillitis , conjunctivitis , fever of unknown origin , suspected encephalitis ; in hiv - positive children : suspected intoxication with traditional medicine , asthma / bronchitis , impetigo , submandibular abscess , keratitis , suspected encephalitis . defined as urine output < 0.5  ml / kg / h for > 24  h  or blood urea nitrogen > 60  mg / dl . comorbidity , complications , and outcome according to hiv status abbreviations : cxr , chest x - ray ; hiv , human immunodeficiency virus . loose stools/24 h.   other significant comorbidities in hiv - negative children included : suspected intoxication with traditional medicine , suspected hepatitis , burn of hand ( 1% ) , asthma / bronchitis , undefined skin rash , reactive arthritis , parasitosis ( tungiasis , ascaris ) , herpes simplex virus labial ulcera , acute otitis media , tonsillitis , conjunctivitis , fever of unknown origin , suspected encephalitis ; in hiv - positive children : suspected intoxication with traditional medicine , asthma / bronchitis , impetigo , submandibular abscess , keratitis , suspected encephalitis . defined as urine output < 0.5  ml / kg / h for > 24  h  or blood urea nitrogen > 60  mg / dl . in surviving children without neurological sequelae , median hospitalization time ( interquartile range [ iqr ] ) was 5 ( 47 ) days in hiv - coinfected versus 4 days ( 35 ) in hiv - negative children ( p  =  .0012 ) . the mortality in hiv - coinfected children was 19/74 ( 26% ) versus 53/581 ( 9% ) in hiv - uninfected children ( p  <  .001 ) . strictly  defined severe malaria ( see the  methods  section ) were included in the analysis . although this substudy was not powered to look at the treatment effect of artesunate versus quinine , the mortality in hiv - positive children treated with artesunate was 22.2% versus 31% with quinine ( odds ratio [ or ] ( 95% ci ) 0.63 [ .221.85 ] ; p  =  .40 ) and in hiv - negative children 8.1% versus 10.1% ( or for artesunate 0.78 [ 0.441.38 ] ; p  =  .39 ) . stratification according to concomitant antiretroviral treatment ( n  =  5 ) or antituberculosis treatment ( n  =  5 ) did not alter any of the results ( data not shown ) . in a logistic regression model adjusted for age and antimalarial treatment , with death as dependent variable and established predictors of severe malaria as independent variables , significant predictors in the final model ( n  =  482 ) were renal impairment ( log bun ) , acidosis ( be ) , parasitemia ( log parasitemia ; but an inverse correlation ) , and plasma pfhrp2 concentration ( table  4 ) . hiv infection was correlated with increased mortality ( unadjusted or 3.44 ; 95% ci 1.886.28 ) , but was not an independent prognosticator when plasma pfhrp2 was introduced into the model , which was related to the correlation between hiv status and plasma pfhrp2 ( test for trend across ordered groups ; p  < the independent predictors were identical in a model including categorical hiv immunological stages rather than presence of hiv infection as a binary variable . also , hiv status did not contribute significantly to the final model when introduced as an interaction term with plasma pfhrp2 concentration . table  4.logistic regression analysis for children , adjusted for age , showing the prognostic value of significant risk factors assessed on admission for in - hospital survival of children with severe falciparum malariavariableodds ratio ( 95% ci)p valueplasma base excess0.89 ( .84.94)<.001[log ] blood urea nitrogen3.81 ( 1.907.65)<.001[log ] parasitemia0.66 ( .47.89).007[log ] plasma pfhrp2 ( squared)1.47 ( 1.101.95).008[log ] plasma pfhrp20.12 ( .03.48).003antimalarial drug ( artesunate vs quinine)0.37 ( .18.78).009the association between death and [ log ] plasma pfhrp2 was u - shaped and best described using a quadratic function . the patients with low pfhrp2 concentrations signify children with a low parasite burden where severe illness is likely caused by an alternative diagnosis than severe malaria ( hendriksen et al . introduction of an interaction term ( hiv-1 status    plasma pfhrp2 ) did not improve the model ( p  =  .88 ) . r=  0.29 for the final model ( n  =  482).abbreviations : ci , confidence interval ; hiv , human immunodeficiency virus ; pfhrp2 , plasmodium falciparumhistidine - rich protein-2 . logistic regression analysis for children , adjusted for age , showing the prognostic value of significant risk factors assessed on admission for in - hospital survival of children with severe falciparum malaria the association between death and [ log ] plasma pfhrp2 was u - shaped and best described using a quadratic function . the patients with low pfhrp2 concentrations signify children with a low parasite burden where severe illness is likely caused by an alternative diagnosis than severe malaria ( hendriksen et al . introduction of an interaction term ( hiv-1 status    plasma pfhrp2 ) did not improve the model ( p  =  .88 ) . abbreviations : ci , confidence interval ; hiv , human immunodeficiency virus ; pfhrp2 , plasmodium falciparumhistidine - rich protein-2 . expert slide readings with quantitative results were available from 575/655 ( 88% ) of enrolled children with known hiv status . geometric mean ( 95% ci ) parasitemia was 47  140 ( 37  98858  498 ) in hiv - negative ( n  =  510 ) and 67  977 ( 37  143124  408 ) in hiv - positive children ( geometric mean ( 95% ci ) plasma pfhrp2 concentration assessed in 653/655 ( > 99% ) children was 831 ( 707975 )  ng / ml in hiv - negative ( n  =  510 ) and 1395 ( 9112136 )  ng / ml in coinfected children ( n  =  65 ; p  =  .0321 ; figure  2 ) . figure  2.comparison of the circulating peripheral blood parasitemia ( left y - axis ) and plasma pfhrp2 concentration as a measure of the total body parasite burden ( right y - axis ) , between human immunodeficiency virus ( hiv)negative (  , n  =  510 ) and hiv - positive (  , n  =  65 ) children with quantified plasma pfhrp2 and peripheral blood parasitemia . abbreviations : ci , confidence interval ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . comparison of the circulating peripheral blood parasitemia ( left y - axis ) and plasma pfhrp2 concentration as a measure of the total body parasite burden ( right y - axis ) , between human immunodeficiency virus ( hiv)negative (  , n  =  510 ) and hiv - positive (  , n  =  65 ) children with quantified plasma pfhrp2 and peripheral blood parasitemia . abbreviations : ci , confidence interval ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . hiv - coinfected children were older and differed in their clinical presentation from uninfected children with an increased frequency of severe acidosis and severe anemia ( both clinically and laboratory assessed ) and respiratory distress ( tables  1 and 2 ) . blood urea nitrogen ( bun ) concentrations were also higher in hiv - infected children . on physical examination , hiv - positive children had significantly lower weight - for - age z scores and more frequent oral candidiasis and lymphadenopathy . in the adult patients , table  2.clinical examination and laboratory assessments in patients with severe malaria according to hiv statusassessmentchildren < 15  yearsadults 15  yearshiv negative ( n  =  581)hiv positive ( n  =  74)p valuehiv negative ( n  =  19)hiv positive ( n  =  49)p valueclinical examinationweight - for - age z score1.0 ( 1.3)1.6 ( 1.1).0001    temperature ( c)38.2 ( 0.9)38.3 ( 1.0).7638.1 ( 1.2)38.2 ( 1.3).76blood pressure ( mmhg )  systolic103 ( 16)104 ( 16).48112 ( 16)113 ( 22).82  diastolic64 ( 13)65 ( 16).6569 ( 14)69 ( 13).89respiratory rate ( breaths / min)39 ( 10)39 ( 9).9826 ( 5)29 ( 8).14coma depth ( n , median , range )  bcs70 , 2 ( 05)7 , 2 ( 23).29     gcs511 , 8 ( 315)67 , 9 ( 315).28718,10 ( 314)48 , 8 ( 315).54oral candidiasis1 ( < 1%)9 ( 12%)<.00104 ( 9%).57lymphadenopathy16 ( 3%)20 ( 27%)<.0011 ( 6%)5 ( 10%).54laboratory assessments  bun ( mg / dl)12 ( 1112)16 ( 1319)<.000123 ( 1535)32 ( 2540).18  hemoglobin ( g / dl)8.2 ( 2.7)7.5 ( 2.7).03311.3 ( 2.6)10.2 ( 3.2).16  ph7.40 ( 0.11)7.38 ( 0.14).267.39 ( 0.09)7.37 ( 0.13).64  hco3 ( mmol / l)19.9 ( 4.9)17.7 ( 6.6).026721.8 ( 3.1)19.7 ( 5.7).17  base excess ( mmol / l)5 ( 6)7 ( 8).04613 ( 4)6 ( 8).43  slide pf positive547 ( 99%)72 ( 99%)1.018 ( 100%)45 ( 96%)1.0parasitemia ( parasites/l ) geometric mean ( 95% ci)47  141 ( 38  00558  474)68  320 ( 37  680123  874).25133  653 ( 59  082302  343)61  525 ( 24  628153  704).36pfhrp2 ( ng / ml ) geometric mean ( 95% ci)834 ( 712977)1452 ( 9832145).0197457 ( 932236)2471 ( 15094047).0072cd4 percentage  21 ( 1227)  13 ( 626)  cd4 absolute cell count  235 ( 118429)  199 ( 76405)  hiv - associated immunodeficiency  not significant  16 ( 23%)  6 ( 14% )  mild  13 ( 19%)  7 ( 16% )  advanced  12 ( 18%)  8 ( 19% )  severe  27 ( 40%)  22 ( 51%)data are no . ( % ) , mean ( sd ) , or median ( iqr ) , unless otherwise indicated.abbreviations : bcs , blantyre coma score ; bun , blood urea nitrogen ; ci , confidence interval ; gcs , glasgow coma score ; hiv , human immunodeficiency virus ; iqr , interquartile range ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 . for children 10 years ( hiv - negative children , n  =  563 ; hiv - positive children , n  =  67 ) . bun was available in n  =  487 and n  =  67 children , n  =  15 and n  =  45 adults , respectively . hemoglobin was available in n  =  551 and n  =  70 children , n  =  19 and n  =  48 adults , respectively . ph was available in n  =  489 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . hco3 was available in n  =  490 and n  =  68 children , be was available in n  =  488 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . parasitemia reported from all patients with peripheral blood slide count , n  =  511 and n  =  66 children , n  =  14 and n  =  44 adults , respectively . pfhrp2 reported for all patients with pfhrp2 sample n  =  580 and n  =  73 children , n  =  18 and n  =  48 adults , respectively . cd4 percentage reported for hiv - infected children < 5 years ( n  =  34 ) and adults ( n  =  42 ) . cd4 absolute cell counts reported for hiv - infected children 5 years ( n  =  34 ) and adults ( n  =  43 ) . clinical examination and laboratory assessments in patients with severe malaria according to hiv status data are no . ( % ) , mean ( sd ) , or median ( iqr ) , unless otherwise indicated . abbreviations : bcs , blantyre coma score ; bun , blood urea nitrogen ; ci , confidence interval ; gcs , glasgow coma score ; hiv , human immunodeficiency virus ; iqr , interquartile range ; pfhrp2 , plasmodium falciparum histidine - rich protein-2 .   for children 10 years ( hiv - negative children , n  =  563 ; hiv - positive children , n  =  67 ) . bun was available in n  =  487 and n  =  67 children , n  =  15 and n  =  45 adults , respectively . hemoglobin was available in n  =  551 and n  =  70 children , n  =  19 and n  =  48 adults , respectively . ph was available in n  =  489 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . hco3 was available in n  =  490 and n  =  68 children , n  =  13 and n  =  44 adults , respectively . be was available in n  =  488 and n  =  68 children , n  =  13 and n  =  45 adults , respectively . parasitemia reported from all patients with peripheral blood slide count , n  =  511 and n  =  66 children , n  =  14 and n  =  44 adults , respectively . pfhrp2 reported for all patients with pfhrp2 sample n  =  580 and n  =  73 children , n  =  18 and n  =  48 adults , respectively . cd4 percentage reported for hiv - infected children < 5 years ( n  =  34 ) and adults ( n  =  42 ) . cd4 absolute cell counts reported for hiv - infected children 5 years ( n  =  34 ) and adults ( n  =  43 ) .   based on who criteria . cd4 percentages and/or absolute counts were available for 68/74 ( 92% ) of hiv - positive children . increasing hiv - associated immunodeficiency according to the who classification was associated with increasing plasma pfhrp2 concentration ( nonparametric test for trend ; p  =  .022 ) but not with mortality ( p  =  .23 ) . comorbidities during hospitalization were more common in hiv - coinfected children ( 15/74 [ 20% ] ) compared with hiv - negative children ( 51/581 [ 9% ] ; p  =  .002 ) . pneumonia was suspected clinically in 19 ( 3% ) of hiv - negative children versus 9 ( 12% ) in hiv - positive children ( p  <  .001 ) . chest x - rays were sparsely available , and only a minority of cases had a radiologically confirmed diagnosis of pneumonia . although culture facilities were lacking , clinical sepsis was more common in hiv - coinfected children as well as a variety of other comorbidities ( table  3 ) . after admission , hiv - coinfected children developed more severe malaria related complications compared to hiv - negative children ( table  3 ) . hiv - coinfected children received more blood transfusions ( 38/74 [ 51% ] vs 224/581 [ 39% ] ; p =  .034 ) and antibiotics ( 65/74 [ 88% ] vs 447/581 [ 77% ] ; p table  3.comorbidity , complications , and outcome according to hiv statuschildren < 15  yearsadults 15  yearshiv negative ( n  =  581)hiv positive ( n  =  74)p valuehiv negative ( n  =  19)hiv positive ( n  =  49)p valuecomorbidity  suspected pneumonia19 ( 3%)9 ( 12%)<.0011 ( 5%)2 ( 4%)1.0  confirmed by cxr5 ( 26%)4 ( 44%).411 ( 100%)0 ( 0%).3  clinical sepsis9 ( 2%)4 ( 5%).0490 ( 0%)1 ( 2%)1.0  suspected meningitis3 ( < 1%)1 ( 1%).381 ( 5%)01.0  gastroenteritis7 ( 1%)2 ( 3%).2700   other significant comorbidities22 ( 4%)7 ( 9%).02500  complications ( not present on admission )  development of coma3 ( < 1%)2 ( 3%).1011 ( 5%)2 ( 4%)1.0  deterioration coma score22 ( 4%)9 ( 12%).0012 ( 11%)6 ( 12%)1.0  convulsions developing or persisting > 6 hours after admission61 ( 11%)18 ( 24%).0012 ( 11%)3 ( 6%).61  respiratory distress6 ( 1%)7 ( 9%)<.0014 ( 21%)5 ( 10%).25  severe anemia ( < 5  g / dl)12 ( 2%)2 ( 3%).6701 ( 2%)1.0  black water fever13 ( 2%)4 ( 5%).1102 ( 4%)1.0  renal failure3 ( < 1%)2 ( 3%).103 ( 16%)8 ( 16%)1.0outcome  mortality53 ( 9%)19 ( 26%)<.0014 ( 21%)17 ( 35%).38  mortality in  strictly  defined severe malaria53/533 ( 10%)19/66 ( 29%)<.0014/16 ( 25%)17/41 ( 41%).36  neurological sequelae at 28  days6 ( 1%)2 ( 3%).2300  abbreviations : cxr , chest x - ray ; hiv , human immunodeficiency virus . no culture facilities available . defined as > 6 loose stools/24 h. other significant comorbidities in hiv - negative children included : suspected intoxication with traditional medicine , suspected hepatitis , burn of hand ( 1% ) , asthma / bronchitis , undefined skin rash , reactive arthritis , parasitosis ( tungiasis , ascaris ) , herpes simplex virus labial ulcera , acute otitis media , tonsillitis , conjunctivitis , fever of unknown origin , suspected encephalitis ; in hiv - positive children : suspected intoxication with traditional medicine , asthma / bronchitis , impetigo , submandibular abscess , keratitis , suspected encephalitis . defined as urine output < 0.5  ml / kg / h for > 24  h  or blood urea nitrogen > 60  mg / dl . comorbidity , complications , and outcome according to hiv status abbreviations : cxr , chest x - ray ; hiv , human immunodeficiency virus . no culture facilities available .   defined as > 6 loose stools/24 h.   other significant comorbidities in hiv - negative children included : suspected intoxication with traditional medicine , suspected hepatitis , burn of hand ( 1% ) , asthma / bronchitis , undefined skin rash , reactive arthritis , parasitosis ( tungiasis , ascaris ) , herpes simplex virus labial ulcera , acute otitis media , tonsillitis , conjunctivitis , fever of unknown origin , suspected encephalitis ; in hiv - positive children : suspected intoxication with traditional medicine , asthma / bronchitis , impetigo , submandibular abscess , keratitis , suspected encephalitis . defined as urine output < 0.5  ml / kg / h for > 24  h  or blood urea nitrogen > 60  mg / dl . in surviving children without neurological sequelae , median hospitalization time ( interquartile range [ iqr ] ) was 5 ( 47 ) days in hiv - coinfected versus 4 days ( 35 ) in hiv - negative children ( p  =  .0012 ) . the mortality in hiv - coinfected children was 19/74 ( 26% ) versus 53/581 ( 9% ) in hiv - uninfected children ( p  <  .001 ) . strictly  defined severe malaria ( see the  methods  section ) were included in the analysis . although this substudy was not powered to look at the treatment effect of artesunate versus quinine , the mortality in hiv - positive children treated with artesunate was 22.2% versus 31% with quinine ( odds ratio [ or ] ( 95% ci ) 0.63 [ .221.85 ] ; p  =  .40 ) and in hiv - negative children 8.1% versus 10.1% ( or for artesunate 0.78 [ 0.441.38 ] ; p  =  .39 ) . stratification according to concomitant antiretroviral treatment ( n  =  5 ) or antituberculosis treatment ( n  =  5 ) did not alter any of the results ( data not shown ) . in a logistic regression model adjusted for age and antimalarial treatment , with death as dependent variable and established predictors of severe malaria as independent variables , significant predictors in the final model ( n  =  482 ) were renal impairment ( log bun ) , acidosis ( be ) , parasitemia ( log parasitemia ; but an inverse correlation ) , and plasma pfhrp2 concentration ( table  4 ) . hiv infection was correlated with increased mortality ( unadjusted or 3.44 ; 95% ci 1.886.28 ) , but was not an independent prognosticator when plasma pfhrp2 was introduced into the model , which was related to the correlation between hiv status and plasma pfhrp2 ( test for trend across ordered groups ; p  < the independent predictors were identical in a model including categorical hiv immunological stages rather than presence of hiv infection as a binary variable . also , hiv status did not contribute significantly to the final model when introduced as an interaction term with plasma pfhrp2 concentration . table  4.logistic regression analysis for children , adjusted for age , showing the prognostic value of significant risk factors assessed on admission for in - hospital survival of children with severe falciparum malariavariableodds ratio ( 95% ci)p valueplasma base excess0.89 ( .84.94)<.001[log ] blood urea nitrogen3.81 ( 1.907.65)<.001[log ] parasitemia0.66 ( .47.89).007[log ] plasma pfhrp2 ( squared)1.47 ( 1.101.95).008[log ] plasma pfhrp20.12 ( .03.48).003antimalarial drug ( artesunate vs quinine)0.37 ( .18.78).009the association between death and [ log ] plasma pfhrp2 was u - shaped and best described using a quadratic function . the patients with low pfhrp2 concentrations signify children with a low parasite burden where severe illness is likely caused by an alternative diagnosis than severe malaria ( hendriksen et al . introduction of an interaction term ( hiv-1 status    plasma pfhrp2 ) did not improve the model ( p  =  .88 ) . =  482).abbreviations : ci , confidence interval ; hiv , human immunodeficiency virus ; pfhrp2 , plasmodium falciparumhistidine - rich protein-2 . logistic regression analysis for children , adjusted for age , showing the prognostic value of significant risk factors assessed on admission for in - hospital survival of children with severe falciparum malaria the association between death and [ log ] plasma pfhrp2 was u - shaped and best described using a quadratic function . the patients with low pfhrp2 concentrations signify children with a low parasite burden where severe illness is likely caused by an alternative diagnosis than severe malaria ( hendriksen et al . introduction of an interaction term ( hiv-1 status    plasma pfhrp2 ) did not improve the model ( p  =  .88 ) . abbreviations : ci , confidence interval ; hiv , human immunodeficiency virus ; pfhrp2 , plasmodium falciparumhistidine - rich protein-2 . this is the first prospective study to report the different clinical presentations of severe malaria , parasite burden , and mortality in hiv - coinfected patients . it was shown that hiv - coinfected children with severe malaria were more undernourished and presented more frequently with severe acidosis , severe anemia , respiratory distress , and elevated bun concentrations , and similar ( albeit nonsignificant ) trends were found in hiv - infected adults . previous studies carried out in areas of high malaria transmission have reported increased prevalence and severity of severe anemia and a higher 7- , 28- , or 90-day post - admission mortality in hiv - coinfected children without differences in admission parasitemia [ 15 ,  16 ,  36 ] . in addition to these findings , we established that a metabolic acidosis is more frequent in hiv - coinfected children . acidosis in severe malaria has been associated with severe anemia and respiratory distress or deep breathing and is an established strong predictor of mortality in adult as well as pediatric severe malaria [ 31 ,  37 ] . total body parasite burden , measured as plasma pfhrp2 concentrations , was higher in hiv - infected children and positively correlated with the severity of immunosuppression according to who immunological classification . this total parasite burden includes the sequestered parasite burden , which causes impaired microcirculatory flow , an important cause of metabolic acidosis in severe malaria [ 24 ,  25 ] . in line with other studies , hiv - coinfected children with severe malaria were older [ 2 ,  14 ,  16 ] and had higher parasite densities [ 14 ,  38 ] . these results suggest that failure of the acquired immunity in hiv infection leads to a decreased ability to control parasitemia , which increases the risk of developing severe malaria with associated high mortality [ 3 ,  9 ,  10 ,  39 ] . the clinical presentation of malaria in hiv - coinfected children depends on malaria specific immunity , which varies according to age and malaria transmission intensity [ 40 ,  41 ] as well as hiv - related immunosuppression [ 9 ,  11 ,  14 ,  39 ] . although our study design did not allow such comparison , our findings support the hypothesis that hiv - related immunosuppression increases the risk of severe malaria in adults , since the hiv-1 seroprevalence in severe malaria was more than twice the reported the hiv-1 seroprevalence in the adult population . elevated bun concentrations have been reported in hiv - coinfected adults with severe malaria [ 11 ,  43 ] , and renal failure due to malaria - induced acute tubular necrosis has been described as a common complication and cause of death in asian adults [ 44 ,  45 ] . it has been hypothesized that hiv - coinfected adults are more likely to have an hiv - mediated impaired renal function , although normalization of renal function following resolution of the malaria episode has also been described . the significance of elevated bun concentrations in hiv - positive patients , particularly in children , and its association with mortality as also observed in other studies [ 32 ,  33 ] requires further investigation . in the adults , sensitivity of the rdts for diagnosing malaria was remarkably low ( < 50% ) . this was explained by low parasite densities on the peripheral blood slide , below the level of detection of the tests . patients with a negative pfhrp2-based rdt and low peripheral blood parasitemia include those with very low total parasite burdens , where an alternative diagnosis other than malaria is more likely . unfortunately , in the setting of the study , possibilities for establishing accurate alternative diagnoses other than malaria were limited . the mozambican national malaria control program recommends parasitological diagnosis of malaria in adults by rapid diagnostic test , although in referral hospitals , severe malaria is commonly diagnosed by peripheral blood slide . slide - positive rdt - negative severe illness represents patients with low parasitemia warrants diagnosis and treatment of other , possibly hiv - related , alternative illnesses [ 14 ,  46 ,  47 ] . further studies to assess the diagnostic work - up and management of this patient group are needed . comorbidities were more frequent in hiv - coinfected children than in hiv - uninfected children ; notably suspected pneumonia and sepsis . in the aquamat trial site in muheza , tanzania , where blood cultures and confidential hiv testing were performed , 8/38 ( 21% ) of hiv - coinfected children had a positive blood culture versus 45/855 ( 5% ) in hiv - negative children ( p  <  .001 ) with an almost 3-fold increased case fatality rate ( 14/38 ( 37% ) in hiv - coinfected children versus 112/855 ( 13% ) in hiv - uninfected children ( p  <  .001 ) [ unpublished data ; personal communication , ilse hendriksen ] . pediatric severe malaria with hiv coinfection has been associated with an increased risk of non  typhi salmonella ( nts )   and/or gram - negative bacteremia , both leading to an increased risk of death . a study in southern mozambique ( an area with similar high hiv prevalence ) reported an incidence of 5.4% of concomitant bacteremia in pediatric severe malaria with streptococcus pneumoniae being the most frequently identified organism , especially in children with respiratory distress , and was associated with a higher case fatality . case fatality with hiv coinfection was 282% higher in children ( p  <  .001 ) and 64% higher in adults with severe malaria ( p  =  .28 ) . however , in a logistic regression model , hiv infection was not an independent predictor of death when plasma pfhrp2 was included in the regression model , whereas there was a clear correlation between hiv status and plasma pfhrp2 concentration , which is a measure of the total body parasite burden , including the sequestered parasites [ 24 ,  25 ] . this again suggests that hiv - induced immune incompetence compromises control of the malaria parasite burden and thus severity of the infection . it also suggests that this mechanism is more important than hiv - related comorbidity , and underscores the importance of potent antimalarial treatment in these children with parenteral artesunate . in accordance with data from asian settings , convulsions , hypoglycemia , and symptomatic severe anemia were more frequent in children , whereas renal impairment and severe jaundice were more common in adult patients in the current african study . the main limitations of this study include the lack of diagnostic information to assess the additional pathology responsible for increased severe malaria mortality in hiv - coinfected patients . chest x - rays were not routinely performed and blood culture facilities were unavailable at the time of this study . in addition , clinical malaria may lower the cd4 lymphocyte count , which may therefore underestimate the patient 's immunological status . in summary , severe malaria in hiv - coinfected children presents with more severe acidosis , anemia and respiratory distress , more complications , and comorbidity , causing higher mortality and prolonged hospitalization in survivors . hiv coinfection is associated with a higher estimated total parasite burden , which is strongly associated with the observed increased severity . higher case fatality and more frequent complications warrant more intense monitoring and a low threshold for additional investigations to diagnose concomitant invasive bacterial infections , including chest x - ray , blood culture , and lumbar puncture with csf examination . since concomitant pneumonia , sepsis , and severe anemia are common , prompt parenteral antimalarial and antibiotic treatment , and availability of supportive treatments ( including oxygen therapy and blood transfusion ) are of extra importance in this group .
background . 
 severe falciparum malaria with human immunodeficiency virus ( hiv ) coinfection is common in settings with a high prevalence of both diseases , but there is little information on whether hiv affects the clinical presentation and outcome of severe malaria.methods . 
 hiv status was assessed prospectively in hospitalized parasitemic adults and children with severe malaria in beira , mozambique , as part of a clinical trial comparing parenteral artesunate versus quinine ( isrctn50258054 ) . 
 clinical signs , comorbidity , complications , and disease outcome were compared according to hiv status.results . 
 hiv-1 seroprevalence was 11% ( 74/655 ) in children under 15 years and 72% ( 49/68 ) in adults with severe malaria . 
 children with hiv coinfection presented with more severe acidosis , anemia , and respiratory distress , and higher peripheral blood parasitemia and plasma plasmodium falciparum histidine - rich protein-2 ( pfhrp2 ) . during hospitalization , deterioration in coma score , convulsions , respiratory distress , and pneumonia were more common in hiv - coinfected children , and mortality was 26% ( 19/74 ) versus 9% ( 53/581 ) in uninfected children ( p  <  .001 ) . in an age- and antimalarial treatment  adjusted logistic regression model , significant , independent predictors for death were renal impairment , acidosis , parasitemia , and plasma pfhrp2 concentration.conclusions . 
 severe malaria in hiv - coinfected patients presents with higher parasite burden , more complications , and comorbidity , and carries a higher case fatality rate . 
 early identification of hiv coinfection is important for the clinical management of severe malaria .