we retrospectively reviewed the medical records of patients who underwent pterygium excision and conjunctival autografting between january 2008 and may 2010 . the inclusion criteria were as follows : 1 ) a follow - up period of more than 3 months after surgery and 2 ) no immune - related disease , ocular surface disease , or eyelid disease . patients with recurrent pterygium , which is accompanied by symblepharon or limitation of duction , were excluded from the study . the study protocol was approved by the institutional review board at seoul national university hospital and was in accordance with the helsinki declaration . the study sample finally consisted of 46 patients ( 52 eyes ) : 20 patients ( 22 eyes ) in the fibrin glue group and 26 patients ( 30 eyes ) in the suture group . best - corrected visual acuity ( bcva ) , slit - lamp examination , and determination of intraocular pressure were performed after the operation . the degree of growth of the pterygium was evaluated in terms of the following 2 parameters : the length of the invading head from the limbus and density of the pterygium alone . the length of the invading head was calculated using a slit lamp , and the density of the pterygium was graded according to the system used by tan et al . ( type 1 : atrophic , type 2 : mild inflammation , and type 3 : moderate / severe inflammation or showing active growth ) . the conjunctival autograft was sutured with 10 - 0 nylon for patients who underwent surgery between january 2008 and october 2009 , and was attached using fibrin glue ( greenplast ; greencross corp . , yongin , korea ) for those who underwent surgery between november 2009 and may 2010 . a single surgeon ( kmk ) performed all the surgeries . topical anesthesia using 0.5% proparacaine hcl ( alcaine ; alcon laboratories , fort worth , tx , usa ) was applied and 2% lidocaine was injected into the pterygium body . the pterygium was dissected from the apex using a straight beaver blade . the pterygium body and soaked with 0.02% mitomycin c solution was placed onto the deep fibrovascular bundle in the medial canthus for 2 minutes , and the applied area was irrigated with 40 ml of balanced salt solution . the grafted tissue was continuously sutured with 10 - 0 nylon , including 4 interrupted sutures , or it was sealed with fibrin glue . the sutures with 10 - 0 nylon were buried . in the fibrin glue group , each syringe of fibrin glue , which is filled with clottable plasma protein or thrombin solution respectively , was prepared with a 23-gauge needle . these components were applied simultaneously to the exposed sclera , using as little as possible , by pushing the double syringe device at the same time . triamcinolone was injected into the inferior subconjunctival space for a total of 0.3 ml after the operation to reduce postoperative inflammation and prevent pterygium recurrence . the operation duration was considered as the time from when the lid retractor was placed until its removal at the end of surgery . after surgery , all patients were prescribed topical prednisolone ( pred forte ; allergan corp . , irvine , ca , usa ) and topical levofloxacin ( cravit ; santen , osaka , japan ) 4 times daily for 2 weeks . bcva , slit - lamp examination , and determination of intraocular pressure were performed at 1 , 7 , 14 , and 28 days and at 2 to 3 months after the operation . in the suture group , 10 - 0 nylon was stitched at the edges at 14 days after the operation , and the anchoring suture along with the limbus , which was supposed to reduce recurrence , was removed at 28 days after the operation . the anterior segment was photographed at 7 and 28 days after the operation , and corneal topography was examined at 28 days after the operation . two ophthalmologists , who were blinded to which group the anterior segment photographs were from , evaluated the postoperative inflammation of photographs . the degree of postoperative inflammation was classified into 3 grades : mild , moderate , and severe inflammation ( fig . postoperative complications were examined , including dehiscence , dislodge , ridge , sub - graft hemorrhage , chemosis , necrosis , contracture , and granuloma . an additional stitch was taken in the case of a dislodged graft , and severe granulomas were surgically removed when medication could not reduce the granuloma . recurrence of pterygium was defined as newly developed fibrovascular tissue invading the cornea to cross the limbus . chi - square test , independent t - test , paired t - test , mann - whitney u - test , and wilcoxon signed rank test were used to analyze the data . bcva ( decimal visual acuity ) was transferred to the logarithm of the minimum angle of resolution ( logmar ) to be analyzed . a single surgeon ( kmk ) performed all the surgeries . topical anesthesia using 0.5% proparacaine hcl ( alcaine ; alcon laboratories , fort worth , tx , usa ) was applied and 2% lidocaine was injected into the pterygium body . the pterygium was dissected from the apex using a straight beaver blade . the pterygium body and soaked with 0.02% mitomycin c solution was placed onto the deep fibrovascular bundle in the medial canthus for 2 minutes , and the applied area was irrigated with 40 ml of balanced salt solution . the grafted tissue was continuously sutured with 10 - 0 nylon , including 4 interrupted sutures , or it was sealed with fibrin glue . the sutures with 10 - 0 nylon were buried . in the fibrin glue group , each syringe of fibrin glue , which is filled with clottable plasma protein or thrombin solution respectively , was prepared with a 23-gauge needle . these components were applied simultaneously to the exposed sclera , using as little as possible , by pushing the double syringe device at the same time . triamcinolone was injected into the inferior subconjunctival space for a total of 0.3 ml after the operation to reduce postoperative inflammation and prevent pterygium recurrence . the operation duration was considered as the time from when the lid retractor was placed until its removal at the end of surgery . after surgery , all patients were prescribed topical prednisolone ( pred forte ; allergan corp . , irvine , ca , usa ) and topical levofloxacin ( cravit ; santen , osaka , japan ) 4 times daily for 2 weeks . bcva , slit - lamp examination , and determination of intraocular pressure were performed at 1 , 7 , 14 , and 28 days and at 2 to 3 months after the operation . in the suture group , 10 - 0 nylon was stitched at the edges at 14 days after the operation , and the anchoring suture along with the limbus , which was supposed to reduce recurrence , was removed at 28 days after the operation . the anterior segment was photographed at 7 and 28 days after the operation , and corneal topography was examined at 28 days after the operation . two ophthalmologists , who were blinded to which group the anterior segment photographs were from , evaluated the postoperative inflammation of photographs . the degree of postoperative inflammation was classified into 3 grades : mild , moderate , and severe inflammation ( fig . postoperative complications were examined , including dehiscence , dislodge , ridge , sub - graft hemorrhage , chemosis , necrosis , contracture , and granuloma . an additional stitch was taken in the case of a dislodged graft , and severe granulomas were surgically removed when medication could not reduce the granuloma . recurrence of pterygium was defined as newly developed fibrovascular tissue invading the cornea to cross the limbus . chi - square test , independent t - test , paired t - test , mann - whitney u - test , and wilcoxon signed rank test were used to analyze the data . bcva ( decimal visual acuity ) was transferred to the logarithm of the minimum angle of resolution ( logmar ) to be analyzed . there were 7 men and 13 women in the fibrin glue group and 4 men and 22 women in the suture group . the mean age ( sd ) was 63.0 ( 10.5 ) years ( range , 46 to 83 years ) in the former and 56.9 ( 12.0 ) years ( range , 33 to 81 years ) in the latter . the mean head length of the pterygium from the limbus was 2.9 ( 1.7 ) and 2.9 ( 1.5 ) mm in the fibrin glue group and suture group , respectively ( p = 0.882 ) . in both groups , all the pterygiums corresponded to type 2 or type 3 ( p = 0.239 ) . the mean follow - up period was 4.4 ( 2.5 ) months ( 3 to 8 months ) and 9.1 ( 4.4 ) months ( 3 to 20 months ) in the fibrin glue group and suture group , respectively ( p = 0.000 ) ( table 1 ) . preoperative bcva ( logmar ) was 0.27 ( 0.42 ) , and bcva at 1 month after the operation was 0.25 ( 0.39 ) in the entire group ; the difference between these two parameters was insignificant ( p = 0.561 ) . however , corneal astigmatism was significantly reduced in the total group from 3.94 ( 4.28 ) diopter to 1.05 ( 0.99 ) diopter ( p = 0.000 ) ( fig . the reduction in astigmatism was not significantly different between the two groups ( table 2 ) . the operation duration was 27.71 ( 5.22 ) minutes in the fibrin glue group , which was remarkably shorter than that in the suture group ( 43.30 [ 8.18 ] minutes , p = 0.000 ) ( fig . , 9 out of 22 eyes ( 40.9% ) showed moderate to severe conjunctival inflammation in the fibrin glue group , whereas all 30 eyes ( 100% ) in the suture group showed moderate to severe inflammation ( p = 0.000 ) ( fig . 2c ) . after 21 days , conjunctival inflammation almost subsided in both groups , and the degree of conjunctival inflammation was not significantly different between the two groups . the rate of complication , including dehiscence , ridge , proliferative granuloma , sub - graft hemorrhage , chemosis , dislodgement , and contracture , did not significantly differ between the two groups ( table 3 ) . however , granulomas tended to occur more often in the fibrin glue group . . surgical removal of the granuloma and subsequent amniotic membrane transplantation made the irregular surface flat and did not show recurrence during the four months ' follow up ( fig . dehiscences were mainly observed between the nasal margin of the graft and the adjacent conjunctiva , but the gap was very narrow and linear , and all the surfaces were fully epithelized within one week and did not require intervention ( fig . the recurrence rate of corneal pterygium within three months of the surgery was 4.55% ( 1 of 22 eyes ) in the fibrin glue group and 20.0% ( 6 of 30 eyes ) in the suture group ; however , the difference in the recurrence rate was not statistically significant ( p = 0.067 ) . there are several kinds of surgeries that excise the pterygium and repair the conjunctiva . among these , the excision of the pterygium with conjunctival autografting is considered to be the procedure of choice in terms of efficacy and long - term stability . it was reported that the recurrence rate in the case of conjunctival autografting was much lower than that in the case of primary closure or amniotic membrane grafting . adjunctive therapies such as mitomycin c , -irradiation , and excimer laser have also been used to decrease the recurrence rate of pterygium in spite of potentially sight - threatening side effects [ 13 - 15 ] . this is the basis for selecting conjunctival autografting with the use of mitomycin c as our surgical protocol . fibrin glue is a blood - derived product which consists of a fibrinogen component and a thrombin component . recently , its spectrum of use in medicine is broad , including neurosurgery , plastic surgery , surgery in otolaryngology , and eye surgery [ 16 - 18 ] . in ophthalmology , fibrin glue is being used in strabismus surgery , corneal surgery , amniotic membrane transplantation , and conjunctival closure following pterygium [ 19 - 21 ] . in the early 2000s , fibrin glue was introduced to pterygium excision and conjunctival autografting as a substitute for suture . using a questionnaire , uy et al .   found that fibrin glue , when used for attaching conjunctival autografts , caused less postoperative discomfort than 10 - 0 nylon . several other studies also reported similar results , but most of them used questionnaires to evaluate the degree of postoperative symptoms such as pain , photophobia , foreign body sensation , irritation , epiphora , and itching [ 5,7,8,22 - 24 ] . we supported these subjective findings of previous reports by evaluating an anterior segment photograph using an objective grading system . srinivasan et al .   also used standardized slit lamp photographs and demonstrated that fibrin glue produced less postoperative inflammation than suture . our data showed some discrepancy with that reported by srinivasan et al . ; they reported no difference in inflammation between the fibrin glue or suture group at 1 week , but inflammation was reduced significantly in the fibrin glue group after 1 to 3 months . on the contrary , we reported significantly less inflammation in the fibrin glue group than suture group at 1 week , and no difference in inflammation between these groups at 1 month . this disparity may be explained by the use of different suture material in each study . on the other hand , we used non - absorbable 10 - 0 nylon , which can trigger inflammation in the earlier period before removal of the stitches . after the stitches were removed at 10 to 14 days after the operation , there was no suture - related side effect on inflammation , which explains the absence of clinical inflammation at 1 month in our study . nevertheless , our study corresponded well with srinivasan et al .   who stated that fibrin glue was less likely to induce inflammation than suture . we also found that our preliminary animal study using a mouse model showed significantly less inflammation in glued conjunctiva than in 10 - 0 nylon sutured conjunctiva ( data are shown in the supplementary section , fig . the main constituent of fibrin glue is the same as that found in human beings , and hence it is less immunogenic . however , various pathogens such as parvovirus b19 , human immunodeficiency virus , and creutzfeldt - jakob agent may be theoretically transmitted to human beings during surgery . in thoracic surgery , there is a report stating that parvovirus b19 was clinically expressed after using fibrin glue . however , there have been no reports describing postoperative infection after the use of fibrin glue in pterygium surgery . regarding other complications in glue - assisted pterygium surgeries , previous reports did not present much of a difference between glue - assisted and conventional pterygium surgeries . however , we found that dehiscence was greater in glue - assisted pterygium surgery , albeit not significantly greater . most dehiscent cases were related to an earlier learning curve in the initial periods of glue - assisted surgery . first , we transplanted a graft of the same size on the bearing area , but this often resulted in a linear gap at the lateral border because of wound contraction or ocular horizontal movement . later , we transplanted an oversized graft 1 mm larger at all borders than the size of the bearing area . consequently , the dehiscence rate was greatly reduced from 44.4% ( 4 cases out of 9 total cases ) in the first cases to 23.1% ( 3 cases out of 13 total cases ) in the last cases ( p = 0.276 , fisher 's exact test ) . although a small dehiscence of the lateral border appears clinically negligible , a larger gap or concurrent dehiscences in all 3 borders should be considered a cause for concern . in our study , 3 cases of pyogenic granuloma were observed in fibrin glue group and no cases of it in suturing group . this incomplete adaptation between the glued conjunctiva can activate fibroblasts or cause grafts to necrotize . this raises the possibility of increasing the risk of fibrosis in the surrounding graft or recurrence of the pterygium if the graft does not survive . the exact mechanism involved in the formation of pyogenic granuloma has not been completely elucidated , but associations with abnormal vascular endothelial cell growth , cytokine abnormalities , and fibroblasts activation have been postulated . we believe that pyogenic granulomas in the present study were associated with a hyperreactive inflammatory change in the circumstance of abundant vascular endothelial cell growth factors originated from ischemic tissue damage . therefore , we need to take caution not to make maladapted conjunctival wound margins when we use the glue . it can lead fibrosis or recurrence , causing the harms to outweigh the advantages of less inflammation in the glued conjunctiva . the recurrence rate of pterygium , depending on the surgery type such as glue - assisted or suture - assisted autografting , has been a matter of controversy . some reports   documented that the fibrin glue group showed lower recurrence rates than the suture group , whereas other reports   stated the converse . hall et al .   reported similar recurrence rates for the two groups . in our study , the small number of cohort patients and a short follow - up period could not determine the long - term recurrence rate of glue - assisted pterygium surgery . nevertheless , we believe that less inflammation in the earlier postoperative stage may be related to a small chance of recurrence ; this hypothesis corresponds with the result of a previous report on correlation between inflammation and recurrence . our study had a limitation because of the retrospective approach , short postoperative follow - up period , and small cohort . in addition , the results could be affected by several biases such as the remaining sutures for anchoring graft for a month in the suture group , or differences in mean duration of follow up and age between the two groups . the learning curve of the surgeon is also considered as a significant limiting factor because all the surgeries using fibrin glue have been conducted after those using 10 - 0 nylon . nonetheless , it is still noteworthy to report that glue - assisted pterygium surgery is an efficient procedure for asian patients . a prospective and randomized clinical trial would be needed in the future . in conclusion , glue - assisted pterygium surgery shortened the operation duration and reduced early postoperative inflammation as compared to suture - assisted pterygium surgery . the difference in complication rates between the two groups did not reach statistical significance , although dehiscence and pyogenic granulomas were observed more frequently in fibrin glue group . glue - assisted pterygium surgery seems to be another option for the pterygium surgery considering its comparable clinical outcome to suture - assisted pterygium surgery , and the fact that it is associated with less discomfort due to a sutureless wound .
purposeto compare the effect of using fibrin glue or 10 - 0 nylon sutures on the clinical outcome of patients undergoing pterygium excision and conjunctival autografting.methodswe retrospectively reviewed the medical records of 52 eyes from 46 patients who underwent pterygium excision and conjunctival autografting and were followed up for more than 3 months . 
 the operation duration , postoperative inflammation , complications , and recurrence rates were compared between groups of 20 patients ( 22 eyes ) for whom fibrin glue was used ( fibrin glue group ) and 26 patients ( 30 eyes ) for whom suturing was performed with 10 - 0 nylon ( suture group ) in pterygium excision and conjunctival autografting.resultsthe operation duration was 27.71 ( 5.22 ) minutes in the fibrin glue group and 43.30 ( 8.18 ) minutes in the suture group ( p = 0.000 ) . 
 seven days after the operation , the fibrin glue group showed milder conjunctival inflammation than the suture group ( p = 0.000 ) . 
 postoperative complications and corneal recurrence rates were not statistically different between the two groups.conclusionsthe use of fibrin glue in pterygium excision with conjunctival autografting is likely to be a more effective , safer procedure than suturing .