atrial fibrillation ( af ) is the most common arrhythmia in clinical practice , with an estimated prevalence of 0.41% in the general population , increasing with age to 9% in those above 80 years . paroxysmal and permanent af may lead to reduced quality of life and has significant health economic consequences . furthermore , asymptomatic af increases risk for the development of stroke . in spite of this , confirming the aetiology of stroke even after extensive cardiologic assessment is often difficult . for patients with symptomatic drug - refractory af , redo procedures increase success rate , but are costly and include risk of adverse events . increased left atrial ( la ) size is a useful parameter to predict af recurrence and values > 5.05.5 cm indicate limited success . in patients with normal or only mildly increased la size , prediction of arrhythmia recurrence is more difficult . af is most frequently related to coronary artery disease , structural heart disease , hypertension , diabetes mellitus , hyperthyroidism , or simply due to aging . however , in the population with no cardiovascular disease or other known causal factors , the mechanism of af is not fully understood . fibrosis may lead to micro - re - entry circuits that consequently lead to electrical dispersion , which is a known arrhythmogenic factor . importantly , these subtle alterations are undetectable by traditional imaging methods . for these reasons , an echocardiographic marker of paroxysmal af ( paf ) and a predictor of recurrence of af after rfa in patients without apparent cardiac pathology would be of great clinical value . strain echocardiography can accurately assess regional myocardial function and timing , and ventricular strain has been shown to be a marker of cardiac prognosis in several publications . furthermore , recent studies have shown that inhomogeneous timing of contractions , presented as mechanical dispersion ( md ) , is a marker of ventricular arrhythmias . we hypothesized that pronounced la md by myocardial strain is associated with susceptibility to atrial arrhythmias in patients with paf and , therefore , may be an additional tool in arrhythmia risk stratification . furthermore , we aimed to investigate the extent of decrease in la function assessed by strain echocardiography in patients with paf . from january 2012 to march 2014 , 258 patients with paf underwent rfa at our centre . indications for rfa were symptomatic drug - refractory af or intolerance to at least one class i or iii antiarrhythmic medication . of these , 127 ( 45% ) had recurrence of af after first rfa and were accepted for redo rfa , while 158 ( 55% ) did not have recurrence of af . the echocardiographic recordings of these patients were reviewed and included in the study if they had normal systolic ( ef > 50% ) and diastolic lv functions , and normal or only mildly increased la size ( la volume < 42 ml / m ) . exclusion criteria were age < 18 years , pregnancy , structural heart disease , history of previous myocardial infarction , percutaneous coronary intervention and open chest surgery , bundle branch block , moderate to severe valvular dysfunction , moderate to severe left ventricular ( lv ) hypertrophy and ongoing af or other sustained arrhythmias . a subset of 61 patients fulfilled inclusion criteria and were retrospectively included in this study . the medication received at the time of the echocardiographic study was recorded ( table  1 ) . table  1clinical characteristicshealthy individuals ( n = 20)patients without recurrence of af after rfa ( n = 30)patients with recurrence of af after rfa ( n = 31)p - valueage ( years)54  1656  1055  80.8male , n ( % ) 15 ( 75)23 ( 77)24 ( 77)1.0heart rate ( bpm)64  1057  957  11<0.05height ( cm)179  9180  10181  90.8weight ( kg)74  1289  13 * 91  14*<0.001bsa ( m)1.7  0.62.1  0.2 * 2.1  0.2*<0.001bmi ( kg / m)23  228  4 * 28  5*<0.001clinical characteristics in patients with and without recurrent af after rfa  years with paf6.3  6.17.3  6.40.6  hypertension , n ( % ) 10 ( 33)6 ( 19)0.2  sbp ( mmhg)130  16128  150.7  dbp ( mmhg)79  1180  100.8  diabetes mellitus , n ( % ) 1 ( 3)2 ( 7)0.6  coronary artery disease , n ( % ) 0 ( 0)1 ( 3)0.3  hypercholesterolemia , n ( % ) 0 ( 0)2 ( 7)0.2  smoking , n ( % ) 5 ( 17)4 ( 13)0.7cha2ds2-vasc score for af stroke risk  score 0 , n ( % ) 13 ( 43)17 ( 55)0.4  score 1 , n ( % ) 10 ( 33)9 ( 29)0.7  score 2 , n ( % ) 6 ( 20)4 ( 13)0.5  score 3 , n ( % ) 1 ( 3)1 ( 3)1.0medication  beta blockers , n ( % ) 16 ( 53)17 ( 55)0.9  ca antagonist for frequency regulation , n ( % ) 2 ( 7)5 ( 16)0.2  flecainide , n ( % ) 14 ( 47)17 ( 55)0.5  sotalol , n ( % ) 1 ( 3)1 ( 3)1.0  amiodarone , n ( % ) 3 ( 10)5 ( 16)0.5  dronedarone , n ( % ) 4 ( 13)4 ( 13)1.0  warfarin , n ( % ) 25 ( 83)30 ( 97)0.8  dabigatran , n ( % ) 3 ( 10)1 ( 3)0.3  rivaroxaban , n ( % ) 2 ( 7)0 ( 0)0.1  acetylsalicylic acid , n ( % ) 1 ( 3)1 ( 3)1.0  ace inhibitors , n ( % ) 0 ( 0)2 ( 7)0.2  at ii receptor antagonists , n ( % ) 7 ( 23)2 ( 7)0.6  anti - diabetic medication , n ( % ) 1 ( 3)0 ( 0)0.3  statins , n ( % ) 9 ( 30)4 ( 13)0.1  diuretics , n ( % ) 7 ( 23)3 ( 10)0.2  ca antagonist for hypertension , n ( % ) 0 ( 0)1 ( 3)0.3mean  sd , numbers . flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups.anova , analysis of variance ; ace , angiotensin - converting enzyme ; at , angiotensin ; af , atrial fibrillation ; bmi , body mass index ; bsa , body surface area ; dbp , diastolic blood pressure ; rfa , radiofrequency ablation ; sbp , systolic blood pressure.*p < 0.05 compared with healthy individuals . flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups . anova , analysis of variance ; ace , angiotensin - converting enzyme ; at , angiotensin ; af , atrial fibrillation ; bmi , body mass index ; bsa , body surface area ; dbp , diastolic blood pressure ; rfa , radiofrequency ablation ; sbp , systolic blood pressure . * the control group consisted of 20 healthy individuals from the hospital staff , matched for age and sex with the af patients . all individuals in the control group had normal electrocardiogram ( ecg ) , physical examination , echocardiographic study , and were free from disease with potential impact on the cardiovascular system . written informed consent the study complies with the declaration of helsinki and is approved by the regional committee for medical research ethics . patients and control subjects underwent an echocardiographic study ( vivid 7 and e9 , ge , vingmed , horten , norway ) . cineloops from three standard apical views ( four chamber , two chamber , and apical long axis ) were recorded using greyscale harmonic imaging . data were digitally stored for offline analysis ( echopac , ge , vingmed ) . from 2d echocardiography , the following parameters were assessed : lv end - diastolic and end - systolic diameters , volumes and ejection fraction by simpson 's biplane method using manual tracing of digital images , lv diastolic function parameters including mitral e and a velocities , e / a ratio , deceleration time , e , e / e , la parameters including diameter , and area in two- and four - chamber views . the endocardial borders were manually traced using a point - and - click technique in the 2d images from the three apical views for longitudinal la and lv myocardial deformation . speckles were tracked frame by frame throughout the la wall during the atrial systole and the lv wall during ventricular systole . peak negative longitudinal myocardial strain by 2d speckle tracking echocardiography was assessed in 18 la and lv segments and averaged to la ( figure  1 ) and lv global longitudinal strain ( la gls and lv gls , respectively ) . contraction duration ( figure  2 ) was measured as time from peak of the p wave on ecg to maximum la shortening by strain . standard deviation of contraction durations in 18 la segments was defined as la md . figure  1upper and lower panels demonstrating apical long - axis views ( apical long - axis , two - chamber , and four - chamber views ) with the left atrium as region of interest . upper panels show a healthy individual with normal atrial deformation , while lower panels demonstrate reduced atrial deformation during atrial systole in a patient with paf but in sinus rhythm during the study . figure  2strain curves and md from the left atrium during atrial systole in a healthy individual ( left panel ) and a patient with paf ( right panel ) but in sinus rhythm during the study . horizontal white arrow indicates contraction duration defined as the time from peak of the atrial p wave on ecg to maximum myocardial shortening . af , atrial fibrillation ; la , left atrial ; mvc , mitral valve closure . upper and lower panels demonstrating apical long - axis views ( apical long - axis , two - chamber , and four - chamber views ) with the left atrium as region of interest . upper panels show a healthy individual with normal atrial deformation , while lower panels demonstrate reduced atrial deformation during atrial systole in a patient with paf but in sinus rhythm during the study . strain curves and md from the left atrium during atrial systole in a healthy individual ( left panel ) and a patient with paf ( right panel ) but in sinus rhythm during the study . horizontal white arrow indicates contraction duration defined as the time from peak of the atrial p wave on ecg to maximum myocardial shortening . af , atrial fibrillation ; la , left atrial ; mvc , mitral valve closure . a 6-f decapolar catheter ( biosense - webster , inc . , ca , usa ) was positioned via the right femoral vein into the coronary sinus . a double transseptal puncture was performed with the introduction of two sl0 sheaths ( st . , ca , usa ) and a 3.5-mm tip irrigated ablation catheter ( navistar thermocool , biosense - webster , inc . , a 3d electroanatomical map ( fam - map ) was created using the carto-3 system ( biosense - webster , inc . , the ablation strategy was encircling of ipsilateral pairs of pulmonary vein antra using a maximum power of 3035 watts . if needed to complete pulmonary vein isolation , additional lesions were given at the carina . the end point of the procedure was pulmonary vein isolation including exit block verified by the lasso catheter . patients were assessed at 3 , 6 , and 12 months after rfa , including outpatient visits with clinical evaluation , serial ecg , and 24-h holter monitoring to detect asymptomatic af episodes . antiarrhythmic medication was continued for at least 3 months after rfa and then discontinued if no af recurrences were detected and no subjective af episodes occurred . oral anticoagulation was continued for at least 6 months ; further continuation of anticoagulation therapy was based on cha2ds2-vasc cardioembolic risk score . the procedure was considered successful if there was no recurrence of af lasting > 30 s during the follow - up , after a blanking period of 3 months . the  test ( categorical variables ) and unpaired student 's t - test ( continuous variables ) were used to determine differences between two groups . comparisons of means for normally distributed variables were performed by analysis of variance ( anova ) with the bonferroni post hoc correction for multiple comparisons ( spss version 21 , spss , inc . , logistic regression analysis was performed to determine the independent prognostic value of la md for predicting paf in the whole study population ( n = 81 ) . age , la volume , e , la gls , and la md were selected for inclusion in a multivariate logistic regression analysis . the selection of variables was based on statistical significance of p < 0.05 in the univariate logistic regression analysis in addition to age and la volume . the area under the receiver - operating characteristic ( roc ) curve ( auc ) was calculated for la gls and la md . the value closest to the upper left corner of the roc curve determined optimal sensitivity and specificity for the ability of the parameters to discriminate between those with and without arrhythmic events . patients and control subjects underwent an echocardiographic study ( vivid 7 and e9 , ge , vingmed , horten , norway ) . cineloops from three standard apical views ( four chamber , two chamber , and apical long axis ) were recorded using greyscale harmonic imaging . data were digitally stored for offline analysis ( echopac , ge , vingmed ) . from 2d echocardiography , the following parameters were assessed : lv end - diastolic and end - systolic diameters , volumes and ejection fraction by simpson 's biplane method using manual tracing of digital images , lv diastolic function parameters including mitral e and a velocities , e / a ratio , deceleration time , e , e / e , la parameters including diameter , and area in two- and four - chamber views . the endocardial borders were manually traced using a point - and - click technique in the 2d images from the three apical views for longitudinal la and lv myocardial deformation . speckles were tracked frame by frame throughout the la wall during the atrial systole and the lv wall during ventricular systole . peak negative longitudinal myocardial strain by 2d speckle tracking echocardiography was assessed in 18 la and lv segments and averaged to la ( figure  1 ) and lv global longitudinal strain ( la gls and lv gls , respectively ) . contraction duration ( figure  2 ) was measured as time from peak of the p wave on ecg to maximum la shortening by strain . standard deviation of contraction durations in 18 la segments was defined as la md . figure  1upper and lower panels demonstrating apical long - axis views ( apical long - axis , two - chamber , and four - chamber views ) with the left atrium as region of interest . upper panels show a healthy individual with normal atrial deformation , while lower panels demonstrate reduced atrial deformation during atrial systole in a patient with paf but in sinus rhythm during the study . figure  2strain curves and md from the left atrium during atrial systole in a healthy individual ( left panel ) and a patient with paf ( right panel ) but in sinus rhythm during the study . horizontal white arrow indicates contraction duration defined as the time from peak of the atrial p wave on ecg to maximum myocardial shortening . af , atrial fibrillation ; la , left atrial ; mvc , mitral valve closure . upper and lower panels demonstrating apical long - axis views ( apical long - axis , two - chamber , and four - chamber views ) with the left atrium as region of interest . upper panels show a healthy individual with normal atrial deformation , while lower panels demonstrate reduced atrial deformation during atrial systole in a patient with paf but in sinus rhythm during the study . strain curves and md from the left atrium during atrial systole in a healthy individual ( left panel ) and a patient with paf ( right panel ) but in sinus rhythm during the study . horizontal white arrow indicates contraction duration defined as the time from peak of the atrial p wave on ecg to maximum myocardial shortening . af , atrial fibrillation ; la , left atrial ; mvc , mitral valve closure . ca , usa ) was positioned via the right femoral vein into the coronary sinus . a double transseptal puncture was performed with the introduction of two sl0 sheaths ( st . ca , usa ) and a 3.5-mm tip irrigated ablation catheter ( navistar thermocool , biosense - webster , inc . , a 3d electroanatomical map ( fam - map ) was created using the carto-3 system ( biosense - webster , inc . , the ablation strategy was encircling of ipsilateral pairs of pulmonary vein antra using a maximum power of 3035 watts . if needed to complete pulmonary vein isolation , additional lesions were given at the carina . the end point of the procedure was pulmonary vein isolation including exit block verified by the lasso catheter . patients were assessed at 3 , 6 , and 12 months after rfa , including outpatient visits with clinical evaluation , serial ecg , and 24-h holter monitoring to detect asymptomatic af episodes . antiarrhythmic medication was continued for at least 3 months after rfa and then discontinued if no af recurrences were detected and no subjective af episodes occurred . oral anticoagulation was continued for at least 6 months ; further continuation of anticoagulation therapy was based on cha2ds2-vasc cardioembolic risk score . the procedure was considered successful if there was no recurrence of af lasting > 30 s during the follow - up , after a blanking period of 3 months . the  test ( categorical variables ) and unpaired student 's t - test ( continuous variables ) were used to determine differences between two groups . comparisons of means for normally distributed variables were performed by analysis of variance ( anova ) with the bonferroni post hoc correction for multiple comparisons ( spss version 21 , spss , inc . , logistic regression analysis was performed to determine the independent prognostic value of la md for predicting paf in the whole study population ( n = 81 ) . age , la volume , e , la gls , and la md were selected for inclusion in a multivariate logistic regression analysis . the selection of variables was based on statistical significance of p < 0.05 in the univariate logistic regression analysis in addition to age and la volume . the area under the receiver - operating characteristic ( roc ) curve ( auc ) was calculated for la gls and la md . the value closest to the upper left corner of the roc curve determined optimal sensitivity and specificity for the ability of the parameters to discriminate between those with and without arrhythmic events . in total , 61 patients with symptomatic drug - refractory paf were included ( table  1 ) . of these , 30 had no recurrence of af during the follow - up , while 31 had recurrent af after one rfa therapy . there was no difference in stroke risk according to cha2ds2-vasc score ( table  1 ) , procedure time , total rfa time , radiation time , and radiation dose ( table  2 ) between the two groups . the first recurrence of af after rfa occurred at 4  2 months in patients who later on were accepted for a redo rfa . total follow - up for patients without recurrence of af was 16  7 months . table  2rfa characteristicspatients without recurrence of af after rfa ( n = 30)patients with recurrence of af after rfa ( n = 31)p - valueprocedure time ( min)228  61239  530.5total rfa time ( s)2730  10282619  9350.7radiation time ( min)40  2050  260.08radiation dose ( ugym)6831  53106907  47181.0mean  sd . right column shows p - values for student 's t-test.af , atrial fibrillation ; rfa , radiofrequency ablation . patients with paf had higher body weight , greater body mass index and body surface area , and lower heart rate compared with healthy controls ( table  1 ) . no differences in age , sex , weight , height , body mass index , co - morbidity , or medication were observed between those with and without recurrence of af after rfa ( table  1 ) . patients with paf had significantly greater la md and impaired la function by gls compared with healthy controls ( both p < 0.001 ; table  3 ) . importantly , patients with recurrence of af after rfa showed greater la md compared with patients without recurrence ( p < 0.05 ) and controls ( p < 0.001 ; table  3 ) . furthermore , patients without recurrence of af after rfa showed significantly increased la md compared with controls ( p < 0.001 ) . table  3echocardiographic resultshealthy individuals ( n = 20)patients without recurrence of af after rfa ( n = 30)patients with recurrence of af after rfa ( n = 31)p - valueanovaf - testla md ( ms)16  830  12 * 38  14*,**<0.001la gls ( % ) 19  216  3*14  4*<0.001la diameter / bsa ( cm / m)1.7  0.61.8  0.21.8 volume / bsa ( ml / m)29  627  428  30.6lv gls ( % ) 22.5  2.421.7  2.221.8  1.90.4lv ef ( % ) 62  463  461  40.4lv edv / bsa ( ml / m)56  2364  865 100.052lv esv / bsa ( ml / m)21  924  425  50.6ivsd ( mm)9  29  29  20.7lvidd / bsa ( mm / m)24  927  325  30.2lvpwd ( mm)8  29  29  20.8e velocity ( cm / s)70  2060  2070  200.7dt ( ms)206  46196  39206  550.7a velocity ( cm / s)50  2060  1050  100.1e / a ratio1.4  0.61.2  0.51.4  0.50.3e ( cm / s)10  39  29 flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups.af , atrial fibrillation ; anova , analysis of variance ; bsa , body surface area ; dt , deceleration time ; edv , end - diastolic volume ; ef , ejection fraction ; esv , end - systolic volume ; gls , global longitudinal strain ; ivsd , interventricular septum diastole ; md , mechanical dispersion ; la , left atrial ; lv , left ventricle ; lvidd , left ventricular internal diameter diastole ; lvpwd , left ventricular posterior wall diastole ; rfa , radiofrequency ablation.*p < 0.05 compared with healthy individuals.**p < 0.05 compared with patients without recurrence of af after rfa . flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups . af , atrial fibrillation ; anova , analysis of variance ; bsa , body surface area ; dt , deceleration time ; edv , end - diastolic volume ; ef , ejection fraction ; esv , end - systolic volume ; gls , global longitudinal strain ; ivsd , interventricular septum diastole ; md , mechanical dispersion ; la , left atrial ; lv , left ventricle ; lvidd , left ventricular internal diameter diastole ; lvpwd , left ventricular posterior wall diastole ; rfa , radiofrequency ablation . * p < 0.05 compared with healthy individuals . * * p < 0.05 compared with patients without recurrence of af after rfa . patients with paf showed significantly reduced la function assessed by longitudinal myocardial strain compared with controls ( p < 0.01 ) . however , there was no difference in la function by longitudinal strain between patients with and without recurrence of af after rfa ( table  3 ) . la md and la gls were independent predictors of paf in a multivariate logistic regression analysis ( n = 81 ) ( p < 0.05 ; table  4 ) . table  4predictors of arrhythmia in patients with pafunivariate logistic regressionmultivariate logistic regressionor95% cip - valueor95% cip - valueage ( years)1.010.971.060.61.020.891.170.8la volume / bsa ( ml / m)0.950.841.070.40.760.571.030.8e ( cm / s)0.770.600.990.040.760.341.690.5la gls ( % ) 1.401.151.710.0011.621.102.390.02la md ( per 10 ms increase)4.672.1610.1<0.0017.842.1528.70.002bsa , body surface area ; ci , confidence interval ; gls , global longitudinal strain ; md , mechanical dispersion ; la , left atrial ; or , odds ratio . predictors of arrhythmia in patients with paf bsa , body surface area ; ci , confidence interval ; gls , global longitudinal strain ; md , mechanical dispersion ; la , left atrial ; or , odds ratio . the roc analysis demonstrated that la md ( auc = 0.88 ) of 24 ms was the optimal cut - off value to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) with a sensitivity of 77% and a specificity of 85% , while la gls ( auc = 0.79 ) at a cut - off value of 18% had a sensitivity and specificity of 75% , respectively for discriminating patients with paf ( figure  3 ) . 
 figure  3roc curve analyses for the ability of la md and la gls to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) . roc curve analyses for the ability of la md and la gls to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) . there were no differences in lv dimensions or volumes , lv diastolic function parameters including mitral e and a velocities , e / a ratio , deceleration time , e , e / e , or la volumes between the three groups ( table  3 ) . lv ejection fraction and lv gls were within normal range in all groups and with no significant differences ( table  3 ) . assessment of longitudinal strain and md could be performed in 1199 ( 82% ) la segments . intra - observer and inter - observer intra - class correlation was performed in 10 patients and was 0.92 ( p < 0.001 ) and 0.73 ( p < 0.05 ) for la gls measurements and 0.92 ( p < 0.01 ) and 0.84 ( p < 0.01 ) for la md measurements , respectively . patients with paf had significantly greater la md and impaired la function by gls compared with healthy controls ( both p < 0.001 ; table  3 ) . importantly , patients with recurrence of af after rfa showed greater la md compared with patients without recurrence ( p < 0.05 ) and controls ( p < 0.001 ; table  3 ) . furthermore , patients without recurrence of af after rfa showed significantly increased la md compared with controls ( p < 0.001 ) . table  3echocardiographic resultshealthy individuals ( n = 20)patients without recurrence of af after rfa ( n = 30)patients with recurrence of af after rfa ( n = 31)p - valueanovaf - testla md ( ms)16  830  12 * 38  14*,**<0.001la gls ( % ) 19  216  3*14  4*<0.001la diameter / bsa ( cm / m)1.7  0.61.8  0.21.8 0.20.3la area / bsa ( cm / m)8  39  19  10.3la volume / bsa ( ml / m)29  627  428  30.6lv gls ( % ) 22.5  2.421.7  2.221.8  1.90.4lv ef ( % ) 62  463  461  40.4lv edv / bsa ( ml / m)56  2364  865 100.052lv esv / bsa ( ml / m)21  924  425  50.6ivsd ( mm)9  29  29  20.7lvidd / bsa ( mm / m)24  927  325  30.2lvpwd ( mm)8  29  29  20.8e velocity ( cm / s)70  2060  2070  200.7dt ( ms)206  46196  39206  550.7a velocity ( cm / s)50  2060  1050  100.1e / a ratio1.4  0.61.2  0.51.4  0.50.3e ( cm / s)10  39  29 flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups.af , atrial fibrillation ; anova , analysis of variance ; bsa , body surface area ; dt , deceleration time ; edv , end - diastolic volume ; ef , ejection fraction ; esv , end - systolic volume ; gls , global longitudinal strain ; ivsd , interventricular septum diastole ; md , mechanical dispersion ; la , left atrial ; lv , left ventricle ; lvidd , left ventricular internal diameter diastole ; lvpwd , left ventricular posterior wall diastole ; rfa , radiofrequency ablation.*p < 0.05 compared with healthy individuals.**p < 0.05 compared with patients without recurrence of af after rfa . flags for significance are obtained from the post hoc pair - wise comparison using the bonferroni correction when comparing the three groups . af , atrial fibrillation ; anova , analysis of variance ; bsa , body surface area ; dt , deceleration time ; edv , end - diastolic volume ; ef , ejection fraction ; esv , end - systolic volume ; gls , global longitudinal strain ; ivsd , interventricular septum diastole ; md , mechanical dispersion ; la , left atrial ; lv , left ventricle ; lvidd , left ventricular internal diameter diastole ; lvpwd , left ventricular posterior wall diastole ; rfa , radiofrequency ablation . * p < 0.05 compared with healthy individuals . * * p < 0.05 compared with patients without recurrence of af after rfa . patients with paf showed significantly reduced la function assessed by longitudinal myocardial strain compared with controls ( p < 0.01 ) . however , there was no difference in la function by longitudinal strain between patients with and without recurrence of af after rfa ( table  3 ) . la md and la gls were independent predictors of paf in a multivariate logistic regression analysis ( n = 81 ) ( p < 0.05 ; table  4 ) . table  4predictors of arrhythmia in patients with pafunivariate logistic regressionmultivariate logistic regressionor95% cip - valueor95% cip - valueage ( years)1.010.971.060.61.020.891.170.8la volume / bsa ( ml / m)0.950.841.070.40.760.571.030.8e ( cm / s)0.770.600.990.040.760.341.690.5la gls ( % ) 1.401.151.710.0011.621.102.390.02la md ( per 10 ms increase)4.672.1610.1<0.0017.842.1528.70.002bsa , body surface area ; ci , confidence interval ; gls , global longitudinal strain ; md , mechanical dispersion ; la , left atrial ; or , odds ratio . predictors of arrhythmia in patients with paf bsa , body surface area ; ci , confidence interval ; gls , global longitudinal strain ; md , mechanical dispersion ; la , left atrial ; or , odds ratio . the roc analysis demonstrated that la md ( auc = 0.88 ) of 24 ms was the optimal cut - off value to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) with a sensitivity of 77% and a specificity of 85% , while la gls ( auc = 0.79 ) at a cut - off value of 18% had a sensitivity and specificity of 75% , respectively for discriminating patients with paf ( figure  3 ) . figure  3roc curve analyses for the ability of la md and la gls to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) . roc curve analyses for the ability of la md and la gls to discriminate patients with paf ( n = 61 ) from healthy individuals ( n = 20 ) . there were no differences in lv dimensions or volumes , lv diastolic function parameters including mitral e and a velocities , e / a ratio , deceleration time , e , e / e , or la volumes between the three groups ( table  3 ) . lv ejection fraction and lv gls were within normal range in all groups and with no significant differences ( table  3 ) . assessment of longitudinal strain and md could be performed in 1199 ( 82% ) la segments . intra - observer and inter - observer intra - class correlation was performed in 10 patients and was 0.92 ( p < 0.001 ) and 0.73 ( p < 0.05 ) for la gls measurements and 0.92 ( p < 0.01 ) and 0.84 ( p < 0.01 ) for la md measurements , respectively . in this study , we utilized a new method for prediction of recurrence of af by strain echocardiography . la md could discriminate patients with paf with no apparent cardiac dysfunction assessed by traditional imaging methods from healthy individuals . la md was more pronounced in patients with recurrence of af after rfa therapy compared with patients without recurrence . md assessed in the lv has been shown to predict ventricular arrhythmias in patients with cardiomyopathies and after myocardial infarction . the aim of this study was to further develop and apply a similar method to predict the risk of recurrence of af in general and after rfa in particular . we wished to examine if md , a method primarily developed to assess risk of ventricular arrhythmias , also could predict atrial arrhythmias . it is well known that heart failure , structural heart disease , and increased la size are predictors of af recurrence after rfa and are associated with increased la scarring . however , normal cardiac function , a structurally normal heart , and normal la size do not exclude the occurrence of af . therefore , we studied a population practically without cardiovascular disease , and normal or only mildly enlarged la , in order to explore if md could predict af recurrence in these patients where risk stratification tools are lacking . myocardial velocities and myocardial strain based on echocardiography have been introduced as quantitative parameters for regional myocardial motion and deformation . we studied myocardial la deformation to characterize global la function ( figure  1 ) and timing of deformation , which reflects contraction heterogeneity ( figure  2 ) . tsai et al . have shown previously that la function by peak negative strain during atrial systole was impaired in patients with paf and could predict new af episodes . furthermore , a recent study has used the same mathematical formula as our study to assess dyssynchrony in the la . however , providencia et al . focused on dispersion of peak positive la strain and stiffness during the atrial reservoir phase . to quantify la dyssynchrony , standard deviation of time to peak peak positive deformation , the la is stretched mainly due to the venous return from the lungs and functions as a reservoir without active contractions . in our study , similarly to tsai et al . , we examined the la during active atrial systole when depolarization of the la takes place , and therefore electrical dispersion is possible . furthermore , the primary aim of the la conduit phase dyssynchrony study was to predict atrial stasis , whereas our study focuses on risk prediction of af recurrence . even though roc curve analysis for la md and la gls were similar in our study , there was a tendency towards greater sensitivity and specificity of la md in the prediction of af . furthermore , we found difference in la md between patients with and without recurrence of af after rfa , while there was no difference in la gls between these groups . finally , in a multivariate analysis , la md had an almost five times greater odds ratio in the prediction of af compared with la gls . the analyses described above indicate additional information given by la md and supports la md as a more sensitive parameter than la gls . recent results from an animal model subjected to exercise showed an increase in the synthesis of mrna markers of fibrosis , an increased turnover of matrix proteins , and the deposition of collagen in both atria and the right ventricle . likewise , verma et al . demonstrated that la scarring assessed by extensive voltage mapping of the la is a powerful , independent predictor of af recurrence after pulmonary vein antrum isolation finally , an inverse relationship between la fibrosis by late - enhancement magnetic resonance imaging ( mri ) and la strain by echocardiography was related to af burden in studies by kuppahally et al . we suggest that la md may reflect the burden of atrial fibrosis and therefore reflect the mechanism for increased risk of recurrence of af after rfa . in our study population , due to the wide age range ( 3472 years ) with most patients in their 50s , the mechanism of la fibrosis is most likely secondary to a variety of causes including hypertension , diabetes mellitus , coronary artery disease , and probably also endurance training . fibrosis leads to atrial micro - re - entry circuits , which consequently lead to electrical dispersion during depolarization . electrical dispersion also leads to md , which can be detected by strain echocardiography . a simple , bedside , non - invasive method that could predict recurrence of af in general and after rfa in particular would be of great clinical interest . identification of non - invasive markers of la dysfunction with prognostic implications for patients with paf could have major clinical implications with regard to the intensity of medical and invasive treatment in these patients . by early detection of atrial dysfunction in patients with paf , larger clinical trials might clarify whether earlier and more aggressive treatment might slow the progression of atrial dysfunction , reduce af recurrence , and prevent the development of permanent af . however , confirming the aetiology of a stroke is often difficult , even after extensive cardiologic assessment . this could have major clinical implications in the case of asymptomatic paf without electrocardiographic proof , as anticoagulation is often indicated in order to prevent stroke in these patients . prediction of development of paroxysmal and permanent af is pivotal to all cardiologists , due to the inherent risk of embolization and fatal stroke . the echocardiographic methods described in this study can be performed with nearly all last generation echocardiographic scanners and can be learned and utilized easily in a clinical setting . follow - up of patients without recurrence of af was 12 months , which is relatively short as af may reoccur years later . we did not include patients with heart failure , structural heart disease , and increased la size . oslo university hospital is a tertiary hospital for af ablation , and therefore our population did not include patients at first diagnosis , and hence we do not have echocardiographic data from this time point . this measure was chosen because longitudinal strain has been best validated , measurements are reproducible and easily obtained with only a minor increase in procedure duration . in addition , radial strain from the la is difficult to obtain due to the thin atrial wall . the reservoir and conduit phases of the la were not assessed as we focused on the active atrial systole when depolarization and active contraction occur . the magnitude of md in the la is considerably smaller compared with dispersion in the ventricles , potentially reflecting difference in the structures for propagation of electrical impulses . consequently , it is more difficult to differentiate between pathological and normal dispersion in the la . this might be exaggerated in the la segments that are relatively far from the transducer , and consequently the density of echo beams is lower . no late - enhancement mri or voltage mapping was performed in order to quantify atrial fibrosis . myocardial velocities and myocardial strain based on echocardiography have been introduced as quantitative parameters for regional myocardial motion and deformation . we studied myocardial la deformation to characterize global la function ( figure  1 ) and timing of deformation , which reflects contraction heterogeneity ( figure  2 ) . tsai et al . have shown previously that la function by peak negative strain during atrial systole was impaired in patients with paf and could predict new af episodes . furthermore , a recent study has used the same mathematical formula as our study to assess dyssynchrony in the la . however , providencia et al . focused on dispersion of peak positive la strain and stiffness during the atrial reservoir phase . to quantify la dyssynchrony , standard deviation of time to peak peak positive deformation , the la is stretched mainly due to the venous return from the lungs and functions as a reservoir without active contractions . in our study , similarly to tsai et al . , we examined the la during active atrial systole when depolarization of the la takes place , and therefore electrical dispersion is possible . furthermore , the primary aim of the la conduit phase dyssynchrony study was to predict atrial stasis , whereas our study focuses on risk prediction of af recurrence . even though roc curve analysis for la md and la gls were similar in our study , there was a tendency towards greater sensitivity and specificity of la md in the prediction of af . furthermore , we found difference in la md between patients with and without recurrence of af after rfa , while there was no difference in la gls between these groups . finally , in a multivariate analysis , la md had an almost five times greater odds ratio in the prediction of af compared with la gls . the analyses described above indicate additional information given by la md and supports la md as a more sensitive parameter than la gls . recent results from an animal model subjected to exercise showed an increase in the synthesis of mrna markers of fibrosis , an increased turnover of matrix proteins , and the deposition of collagen in both atria and the right ventricle . likewise , verma et al . demonstrated that la scarring assessed by extensive voltage mapping of the la is a powerful , independent predictor of af recurrence after pulmonary vein antrum isolation . finally , an inverse relationship between la fibrosis by late - enhancement magnetic resonance imaging ( mri ) and la strain by echocardiography was related to af burden in studies by kuppahally et al . we suggest that la md may reflect the burden of atrial fibrosis and therefore reflect the mechanism for increased risk of recurrence of af after rfa . in our study population , due to the wide age range ( 3472 years ) with most patients in their 50s , the mechanism of la fibrosis is most likely secondary to a variety of causes including hypertension , diabetes mellitus , coronary artery disease , and probably also endurance training . fibrosis leads to atrial micro - re - entry circuits , which consequently lead to electrical dispersion during depolarization . electrical dispersion also leads to md , which can be detected by strain echocardiography . a simple , bedside , non - invasive method that could predict recurrence of af in general and after rfa in particular would be of great clinical interest . identification of non - invasive markers of la dysfunction with prognostic implications for patients with paf could have major clinical implications with regard to the intensity of medical and invasive treatment in these patients . by early detection of atrial dysfunction in patients with paf , larger clinical trials might clarify whether earlier and more aggressive treatment might slow the progression of atrial dysfunction , reduce af recurrence , and prevent the development of permanent af . however , confirming the aetiology of a stroke is often difficult , even after extensive cardiologic assessment . this could have major clinical implications in the case of asymptomatic paf without electrocardiographic proof , as anticoagulation is often indicated in order to prevent stroke in these patients . prediction of development of paroxysmal and permanent af is pivotal to all cardiologists , due to the inherent risk of embolization and fatal stroke . the echocardiographic methods described in this study can be performed with nearly all last generation echocardiographic scanners and can be learned and utilized easily in a clinical setting . follow - up of patients without recurrence of af was 12 months , which is relatively short as af may reoccur years later . we did not include patients with heart failure , structural heart disease , and increased la size . oslo university hospital is a tertiary hospital for af ablation , and therefore our population did not include patients at first diagnosis , and hence we do not have echocardiographic data from this time point . this measure was chosen because longitudinal strain has been best validated , measurements are reproducible and easily obtained with only a minor increase in procedure duration . in addition , radial strain from the la is difficult to obtain due to the thin atrial wall . the reservoir and conduit phases of the la were not assessed as we focused on the active atrial systole when depolarization and active contraction occur . the magnitude of md in the la is considerably smaller compared with dispersion in the ventricles , potentially reflecting difference in the structures for propagation of electrical impulses . consequently , it is more difficult to differentiate between pathological and normal dispersion in the la . this might be exaggerated in the la segments that are relatively far from the transducer , and consequently the density of echo beams is lower . no late - enhancement mri or voltage mapping was performed in order to quantify atrial fibrosis . we found a dispersed la contraction pattern and reduced la deformation in patients with paf and normal or only mildly enlarged la , and apparently normal lv structure and function when comparing with healthy individuals . la md before rfa treatment was most pronounced in af patients who experienced recurrence of af after rfa . we propose that la md by strain echocardiography may be useful as a marker of paf and as a predictor of af recurrence after rfa . this work was supported by the south - eastern norway regional health authority and by the bergesen foundation . funding to pay the open access publication charges for this article was provided by the research council of norway .
aimswe evaluated if a dispersed left atrial ( la ) contraction pattern was related to atrial fibrillation ( af ) in patients with normal left ventricular ( lv ) function , and normal or mildly enlarged left atrium.methods and resultswe included 61 patients with paroxysmal af ( paf ) . of these 
 , 30 had not while 31 had recurrence of af after radiofrequency ablation ( rfa ) . 
 twenty healthy individuals were included for comparison . 
 echocardiography was performed in patients in sinus rhythm the day before rfa . 
 la volume was calculated . 
 peak negative longitudinal strain was assessed in 18 la segments during atrial systole . 
 contraction duration in 18 la segments was measured as the time from peak of the p wave on electrocardiogram to maximum myocardial shortening in each segment . 
 the standard deviation of contraction durations was defined as la mechanical dispersion ( la md ) . 
 la size was rather preserved in patients with paf ( la volume 25  10 ml / m2 ) . 
 la md was more pronounced in patients with recurrence of af after rfa compared with those without recurrence and controls ( 38  14 ms vs. 30  12 ms vs. 16  8 ms , both p < 0.001 ) . 
 la md was a predictor of paf [ or 7.84 ( 95%ci 2.1528.7 ) , p < 0.01 , per 10 ms increase ] adjusted for age , la volume , e , and la function . 
 la function by strain was reduced in both patients with and without recurrent af after rfa compared with controls ( 14  4% vs. 16  3% vs. 19  2% , both p < 0.05).conclusionla md was pronounced , and la deformation was reduced in patients with paf with apparently normal lv structure and function , and normal or mildly enlarged la . 
 la md may be useful as a predictor of af recurrence after rfa .