paclitaxel is a very potent anticancer drug that has been used since 1967 to treat breast , ovarian , and lung cancer , as well as advanced forms of kaposi 's sarcoma . paclitaxel is a hydrophobic drug ( solubility ~0.3  g / ml )   that was previously administered via dissolution in cremophor el ( polyoxyethylated castor oil ) and dehydrated alcohol . however , the castor oil proved to be toxic [ 3 , 4 ] , so other alternatives for paclitaxel delivery were investigated , such as the use of nanoscale carrier vectors [ 2 , 5 , 6 ] . among the different types of nanocarriers , liposomes have received a lot of attention and have proven to be very good carriers of drugs and/or contrast agents . paclitaxel - embedded buckysomes have the potential for delivering the hydrophobic drug directly to tumor sites . the buckysomes are self - assembled structures of the monomer af-1 , a modified water soluble fullerene that contains 18 carboxylic acid groups and 5 didodecyl malonate chains around the c60 fullerene ( figure 1 ) . this unique functionalization permits the creation of buckysome structures that might offer advantages over traditional phospholipid liposomes as nanovectors . the buckysomes are formulated so they have a dense hydrophobic region , making them ideal for embedding hydrophobic molecules , such as paclitaxel . liposomes are mostly suitable for carrying a hydrophilic payload in their hydrophilic compartment and less suitable for hydrophobic materials . another advantage of using buckysomes is the lack of organic solvents during their formation steps as well as their ease of preparation . previous studies in our group report the synthesis and characterization of buckysome nanovectors for hydrophobic molecule delivery . the therapeutic efficacy of paclitaxel - embedded buckysomes was tested in vitro and compared to that of abraxane , a commercially available albumin - bound formulation of paclitaxel . the paclitaxel - embedded buckysomes demonstrated a similar efficacy to that of abraxane when tested on mcf-7 breast cancer cells , where we observed mitigated cell division at 48 and 72 hours . these results opened the door to study the clearance and biodistribution of paclitaxel - embedded buckysomes in an animal model and study them as possible drug carriers in vivo . thus , in this study we evaluated the clearance and biodistribution of paclitaxel - embedded buckysomes in icr mice and compared the results to those of abraxane . , citrate buffer was purchased from sigma - aldrich , paclitaxel was purchased from vwr , and abraxane was obtained from abraxis oncology . empty buckysomes preparation was described in a previous paper published by our group . briefly , to create buckysomes with a dense , hydrophobic interior , af-1 ( 1.5  mg / ml ) was hydrated in 10  mm citrate buffer ( ph 7.4 ) for 10  min at 70c . during this process , the mixture was subjected to four vortex cycles for 30  s. the four vortex cycles were separated by equal time intervals . to prepare paclitaxel - embedded buckysomes ( pebs ) , 0.6  mg / ml paclitaxel was sonicated ( cole - parmer sonicator ( model no . 08849 - 00 ) , vernon hills , il ) for 1  min in 10  mm citrate buffer ( ph 7.4 ) . af-1 was quickly added to this solution at a concentration of 1.5  mg / ml , and pebs were prepared from the resulting solution using the method described previously . the animal studies were performed on female icr mice ( 2530  g ) ordered from harlan , and they were housed at the university of texas health science center at houston ( uthsch ) . the animal protocol was approved by the uthsch center for laboratory animal medicine and care ( protocol no . : hsc - awc-08 - 137 ,  preclinical development studies on paclitaxel - embedded fullerene nanoparticles  ) ; experiments were performed in accordance with institutional guidelines , and all efforts were made to minimize suffering . the mice were divided into 4 groups : ( 1 ) mice injected with saline ( 250  l ) ( control ) , ( 2 ) mice injected with abraxane ( 250  l , 0.2  mg / ml ) ( positive control ) , ( 3 ) mice injected with empty buckysomes ( 250  l , 2  mg / ml af-1 ) , ( 4 ) mice injected with paclitaxel - embedded buckysomes ( 250  l , 0.2  mg / ml paclitaxel , and 2  mg / ml af-1 ) . mice were injected through the tail vain with the various materials and sacrificed at 15 , 30 , 45 , 60 , and 180 minutes . immediately after sacrifice , at every time point , blood along with kidney , liver , spleen , lungs , heart , and brain were collected and stored at 80c before analysis . the amount of af-1 and/or paclitaxel present in each organ was quantified by hplc after extraction as presented in the supplementary material section ( see supplementary material available online at http://dx.doi.org/10.1155/2013/390425 ) . the unpaired t - test was performed to establish comparison between the control and the paclitaxel groups in peb . the biodistribution of abraxane , paclitaxel in peb , af-1 in empty buckysomes , and af-1 in peb in major tissues was monitored for 180 minutes after iv injection in icr mice . the extraction efficiencies for abraxane were 52 , 37 , 26 , 18 , 24 , 35 , and 37 percent for blood , liver , spleen , heart , kidney , lung , and brain , respectively . as for the paclitaxel in peb , the extraction efficiencies were 48 , 18 , 36 , 54 , 35 , 40 , and 24 percent for blood , liver , spleen , heart , kidney , lung , and brain , respectively . the extraction efficiencies were used for data corrections . the distribution profile for plasma after tail vein injection of a single dose of abraxane , paclitaxel in peb ( 0.2  mg / ml paclitaxel + 2  mg / ml af-1 ) , or af-1 in eb ( 2  mg / ml af-1 ) ( n = 3 for abraxane , n = 5 for pebs , n = 5 for ebs ) . however , there is no measurable amount of paclitaxel in circulation after 180 minutes . in order to study if paclitaxel was still associated with the buckysomes after tail vein injection , the distribution profile for plasma was compared between paclitaxel in peb ( 0.2  mg / ml paclitaxel + 2  mg / ml af-1 ) and af-1 in eb ( 2  mg / ml af-1 ) . the results are presented in figure 2 and clearly demonstrate that paclitaxel and af-1 have the same clearance profile , indicating that paclitaxel was encapsulated in buckysomes . the biodistribution profiles for liver , kidney , spleen , heart , lung , and brain at 15 , 30 , 45 , 60 , and 180 minutes after tail vein injection in icr mice are shown in figure 3 . ( n = 3 for abraxane , n = 5 for peb , p < 0.0001 versus control ; p < 0.05 versus control ) . our results indicated that the buckysomes are cleared rapidly via hepatic and renal pathways ( figures 3(a ) and 3(b ) ) . paclitaxel reaches high concentrations at forty - five minutes and sixty minutes after iv injections of paclitaxel - embedded buckysomes in liver and kidneys . also , the buckysomes exhibit a high peak of paclitaxel in the lungs ( figure 3(e ) ) at 30 minutes after injection . the biodistribution profiles for the other organs and for the other time points indicate that abraxane was a better long - term therapy drug . the concentration of paclitaxel in peb was lower in plasma ( at all - time points ) , kidney ( at 30  min , and 180  min ) , spleen ( at 15 , 30 , 60 , and 180  min ) , heart ( at all - time points ) , lung ( at 60 and 180  min ) , and brain ( at 15 , 45 , 60 , and 180  min ) compared to that in the control . we also get very similar distribution profiles in the previously mentioned organs for both af-1 in eb and paclitaxel in peb ( results presented in supplementary material ) , indicating that paclitaxel is strongly associated with the buckysomes and conforming that the buckysomes are mostly suitable as a short - term delivery carrier . paclitaxel - embedded buckysomes were previously synthesized and characterized in vitro in our lab and seemed to be very promising novel delivery vehicles . in the present study , paclitaxel - embedded buckysomes were characterized in terms of their clearance and biodistribution profile , and the results were compared to those for abraxane . pebs did not exhibit a high plasma concentration , and they were cleared in about 60 minutes by liver and kidneys . the administration of abraxane was associated with higher blood concentrations as compared to that of paclitaxel - embedded buckysomes with measurable amounts of paclitaxel in abraxane at 180 minutes . the biodistribution of paclitaxel in pebs for other organs also demonstrated that , overall , abraxane proved to be a better long - circulating drug . however , pebs showed higher tissue accumulation in the liver and kidney at 45 and 60 minutes and in the lungs at 30 minutes , making them suitable drug - delivery carriers for short - term therapy to the mentioned organs . our results also clearly indicate that paclitaxel was successfully encapsulated in the hydrophobic interior of buckysomes as the blood clearance profile as well as the organ biodistribution for paclitaxel in paclitaxel - embedded buckysomes is similar to that of af-1 in empty buckysomes . future studies will focus on targeting the paclitaxel - embedded buckysomes to subcutaneously implanted tumors and study their efficacy as compared to the commercially available abraxane .
buckysomes , liposome - like vesicles comprised of dendritic c60 subunits that self - assemble into unilamellar vesicles , are unique nanovectors that have utility in drug delivery . 
 we have prepared paclitaxel - embedded buckysomes ( pebs ) and examined biodistriubition profiles with commercially available formulations of the drug . as compared to abraxane , an albumin - bound formulation of paclitaxel , pebs showed higher tissue accumulation in the liver and the kidney at 45 and 60 minutes and in the lungs at 30 minutes , making them suitable drug - delivery carriers for short - term therapy to the mentioned organs . 
 these buckysomes can be further functionalized to specifically deliver paclitaxel to the tumor site .