a 57-year - old male presented with a 3-day history of worsening floaters and reduced visual acuity . fundoscopy and optical coherence tomography showed presence of fluffy white preretinal and intraretinal infiltrates . with no past medical history or evidence of immunosuppression but having travelled abroad and suffered from diarrhoea , fungal aetiology was thought to be unlikely and as a result , treatment was commenced for toxoplasma . despite treatment , initial investigations including inflammatory markers , serology for toxoplasmosis , blood culture , chest radiograph and aqueous sampling could not identify a source of infection . however , polymerase chain reaction results from vitreous sampling revealed c. albicans . as a result , the patient was treated with intravenous voriconazole and intravitreal amphotericin b. as initial clinical improvement was limited , a vitrectomy was performed with further intravitreal amphotericin b. clinical improvement was rapid following vitrectomy . after repeated gram staining and culture of infected toenails , gram - positive yeast cells were isolated . although c. albicans is a frequent cause of endogenous endophthalmitis , patients often have one or more predisposing systemic condition assisting the diagnosis . the present case illustrates that ( 1 ) even in the absence of any predisposing risk factors , c. albicans should be considered as a possible differential diagnosis in recalcitrant uveitis , and ( 2 ) endogenous candida endophthalmitis can be a result of fungal infections from distant sites such as the toenails . it presents as a complication in 945% of all cases of candidaemia ( commonly with candida albicans ) , associated with well - known predisposing risk factors such as intravenous drug abuse , immunodeficiency , prolonged systemic corticosteroid or antibiotic therapy , cytotoxic chemotherapy and long - term parenteral feeds [ 1 , 2 ] . in rare cases of endogenous infection with no presdisposing risk factors , diagnosis and justification for use of intravitreal antifungal agents this is a case report describing the management of a patient with endogenous endophalmitis due to c. albicans which was not associated with any of the known predisposing factors of candidaemia . the report demonstrates the development of a differential diagnosis in an atypical scenario , the eventual confirmation of the infective agent from vitreous sampling and also the identification of the possible source of the candida organism leading to the justification for an aggressive antifungal regimen which resulted in a good outcome . a 57-year - old male presented with a 3-day history of blurred vision , floaters and reduced visual acuity to 6/9 in his left eye . his right eye was asymptomatic with a visual acuity of 6/5 . ocular examination of the left eye showed inflammatory activity in the anterior and posterior segments with mild anterior uveitis and vitritis along with several fluffy white retinal infiltrates near the foveal centre ( fig . an optical coherence tomography ( oct ) scan confirmed the presence of vitreous and intraretinal infiltrates ( fig . further clinical evaluation revealed that the patient had been fit and well but had a brief episode of diarrhoea several weeks earlier whilst visiting the canary islands , spain , and he also had developed signs of toenail infection affecting several toes over the previous 6 months . he was not on any medication and there was no history of other illness , intravenous drug abuse or any opportunistic infections to suggest immunosuppression . clinical suspicion at this stage was of infective retinitis due to fungal infection or toxoplasmosis . because the patient was not immunocompromised , a fungal aetiology was felt to be less likely and he was commenced on oral azithromicin 500 mg once a day and hourly topical dexamethasone 0.1% . as this was an empirical treatment , an extensive uveitis screen was performed , but all of these initial tests were normal or negative , including chest x - ray , haematological profile , esr , crp , serum ace level , serology for toxoplasma and vdrl as well as hiv . polymerase chain reaction ( pcr ) analysis of aqueous was also negative for toxoplasma , cmv , vzv , hsv , mycobacterium , candida and fungal rna . after 1 week of follow - up , the patient had worsened considerably with increasing vitritis and vitreous infiltrates at the vitreoretinal interface causing a reduction in vision to 6/60 ( fig . an automated vitrector was used to obtain a vitreous biopsy and samples were sent for pcr analysis and microbiological testing . from this sample , pcr analysis was positive for c. albicans dna , which was confirmed several weeks later by a positive culture . following confirmation of candida dna on pcr analysis of the vitreous sample , intravenous voriconazole 400 mg b.d . and after 7 days of systemic antifungal therapy with the above regimen , there was very little treatment response and hence a 3-port pars plana vitrectomy was performed and intravitreal administration of amphotericin b ( 5 g/0.1 ml ) was repeated . diagnostic sampling was repeated on this occasion and although pcr analysis was negative for candida dna from the second vitrectomy sample , culture yielded positive growth and histopathological analysis showed typical yeast - like organisms with the classical budding pattern appearance ( fig . the further confirmation of candida on a second vitreous biopsy justified continuing antifungal therapy with oral voriconazole ( 200 mg b.d . ) . the confirmation of the source of the endogenous candida endophthalmitis came much later when repeated samples of nail clippings eventually detected gram - positive yeast cells 30 days after the initial presentation . the patient improved following the vitrectomy and there were no signs of vitreous infiltrates ( fig . after further 2 months of follow - up , his vision had improved to 6/9 with complete resolution of retinal oedema . the diagnostic approach in this case was influenced by a strong , pretest clinical suspicion of an underlying infective aetiology due to the absence of systemic inflammatory signs , cellular infiltration in both anterior and posterior segments , unilateral involvement and the presence of focal infiltration in the posterior vitreous and anterior retinal layers . the organisms involved in cases of infective uveitis depend largely on whether the patient is immunocompromised or immunocompetent . fungal and infections by other opportunistic pathogens are much more likely to occur in immunocompromised individuals . c. albicans is the most common cause of endogenous endophthalmitis and frequently presents as a unilateral posterior uveitis associated with fluffy white vitreous and retinal infiltrates which have often been described as a  string of pearls appearance . it almost always occurs in patients who are immunocompromised or have other predisposing risk factors such as intravenous drug abuse [ 2 , 3 ] . although our patient had a very typical fundus appearance suggestive of fungal endophthalmitis , it was very unusual in that he was immunocompetent and had no apparent risk factors . in the world literature , there are only two case reports of endogenous candida endophthalmitis with no previous predisposing risk factors [ 5 , 6 ] . the culture for the same strain of candida was positive for the nail and the eye . the second case had used inhaled steroids for asthma and could have developed asymptomatic oropharyngeal candidiasis . from these reported cases and the experience in our case , it appears that endogenous candida endophthalmitis could occur from mild fungal infections from a distant site such as the toenail . another interesting and unexpected finding in this case was the negative pcr result from the vitreous sample taken at the second vitrectomy when this specimen eventually grew candida on culture . this paradoxical disconnect between pcr and culture results has been reported before , especially in association with repeated sampling . analysed all pcr results of aqueous and vitreous samples in diagnosis of infectious uveitis and concluded that specimens from the first week of presentation ( acute stage ) were more likely to have positive pcr results than those sampled later . postulate polymorphism and specimen degradation as possible causes of false - negative results . in our case , the first yielded positive pcr , but a positive growth on culture took over 3 weeks by which time the second vitrectomy had been performed . the second vitreous sample yielded a negative pcr , but yeast - like organisms were identifiable on histopathology and growth was also positive on culture . this illustrates that confirmation of candida as the causative agent can be difficult as pcr and gram stain can be falsely negative and culture can take several weeks to yield any diagnostic growth . reported cases of endogenous endophthalmitis due to c. albicans tend to have a poor visual outcome . our case had a good visual outcome despite the infiltrate being located very close to the fovea . the factors contributing to the good outcome in this case were probably the early intervention with a second vitrectomy and intravitreal amphotercin b , the continuation of prolonged course of oral voriconazole and also the immunocompetent status of our patient . systemic antifungal drugs such as fluconazole , voriconazole and amphotericin b given intravenously or orally are indicated in disseminated candidaemia . we chose to use voriconazole as a first - line antifungal for its broad spectrum activity as we did not have confirmation of candida on the vitreous culture initially and blood cultures were negative . several reports have suggested early vitrectomy , especially in lesions affecting the macula or significant vitritis . advantages are thought to be reduction of infectious load , increase in intraocular diffusion of systemically administered medication and reduction of the risk of retinal detachment by limiting vitreoretinal traction [ 9 , 10 ] . for these multiple potential benefits , we felt it was justified to perform a vitrectomy and allowed the intravitreal administration of amphotericin b ( 5 g/0.1 ml ) which tends to have poor ocular penetration when given systemically [ 11 , 12 ] . in summary , this interesting case illustrates that endogenous endophthalmitis due to c. albicans is rare but can occur in patients with no predisposing risk factors or evidence of candidaemia . in these situations , when the clinical findings suggest a fungal aetiology , repeated diagnostic testing with a combination of pcr , gram staining and culture of vitreous and suspected primary sites of infection can be helpful in confirming the definitive diagnosis and provide confidence for adherence to a prolonged course of systemic antifungal therapy to achieve eradication of the organism and a good visual outcome .
purposeto report a case of candida albicans endophthalmitis with no identifiable predisposing risk factors.case reporta 57-year - old male presented with a 3-day history of worsening floaters and reduced visual acuity . 
 fundoscopy and optical coherence tomography showed presence of fluffy white preretinal and intraretinal infiltrates . with no past medical history or evidence of immunosuppression but having travelled abroad and suffered from diarrhoea , fungal aetiology was thought to be unlikely and as a result , treatment was commenced for toxoplasma . despite treatment , 
 his vision did not improve . 
 initial investigations including inflammatory markers , serology for toxoplasmosis , blood culture , chest radiograph and aqueous sampling could not identify a source of infection . however , polymerase chain reaction results from vitreous sampling revealed c. albicans . as a result , 
 the patient was treated with intravenous voriconazole and intravitreal amphotericin b. as initial clinical improvement was limited , a vitrectomy was performed with further intravitreal amphotericin b. clinical improvement was rapid following vitrectomy . 
 after repeated gram staining and culture of infected toenails , gram - positive yeast cells were isolated.conclusionalthough c. albicans is a frequent cause of endogenous endophthalmitis , patients often have one or more predisposing systemic condition assisting the diagnosis . 
 the present case illustrates that ( 1 ) even in the absence of any predisposing risk factors , c. albicans should be considered as a possible differential diagnosis in recalcitrant uveitis , and ( 2 ) endogenous candida endophthalmitis can be a result of fungal infections from distant sites such as the toenails .