during 2008 , more than 500 bats associated with possible human exposure were submitted to the maryland department of health and mental hygiene state laboratory for postmortem diagnosis of rabies by direct fluorescent antibody assay ( technical appendix ) . for this study , virus particles were purified from the lungs and livers of 120 rabies - negative bats with good carcass condition , and viral nucleic acids were extracted , randomly amplified , and sequenced by using 454 pyrosequencing ( roche , mannheim , germany ) and solexa illumina sequencing ( illumina , san diego , ca , usa ) . a total of 100,000 pyrosequences and 13.5 million solexa illumina sequences were generated and then assembled to form contigs . more than 30 contigs showed low protein identities to known vesiculoviruses ( blastx ; http://blast.ncbi.nlm.nih.gov/blast.cgi ) , indicating a possible novel virus . pcr and rapid amplification of cdna ends were performed to obtain the complete genome of this virus ( primers shown in technical appendix figures 1 , 2 ) . we proposed the name american bat vesiculovirus ( abvv ) for this virus .   the full - length abvv genome ( genbank accession no . jx569193 ) consists of 10,692 nt of negative - sense , single - stranded rna beginning with a 103-nt 5 untranslated region , followed by open reading frames encoding for the nucleoprotein , phosphoprotein , matrix protein , glycoprotein , and polymerase protein genes ( figure , panel a ) . phylogenetic analyses showed that abvv is related to vesiculoviruses including chandipura virus and isfahan virus , both of which are associated with encephalitic illness in humans . abvv is located close to the root of vesiculoviruses in the bayesian analysis of the nucleoprotein gene ( figure , panel b ) and shares 41%49% aa identity with known vesiculoviruses , similar to the vesiculovirus interspecies identities reported ( 47.9%72.5% ) and higher than the intergenera identities between vesiculoviruses , lyssaviruses , and ephemeroviruses ( 17.0%33.1% ) ( 14 ) . analyses of the polymerase gene alone ( technical appendix figure 3 ) and of a concatenation of all 5 genes ( figure , panel c ) suggested that abvv lies within the vesiculovirus clade , more closely related to mammalian than fish vesiculoviruses . combined , these analyses indicate that abvv is likely to belong to the vesiculovirus genus , rather than representing a novel genus . lung and liver tissues from 60 of the bats used for the pooled metagenomic analyses were screened individually for abvv by using reverse transcription pcr targeting the polymerase gene ( technical appendix ) . three ( 5% ) bats tested positive for abvv : 1 adult female , 1 adult male , and 1 juvenile male . viral rna was found in liver tissue from the 2 male bats and in lung and liver tissues from the female adult bat . considering the extensive lyssavirus diversity in bats , we hypothesize that bat vesiculoviruses are similarly diverse . to facilitate characterization of diverse vesiculoviruses in bats , we designed 2 pairs of degenerate pcr primers ( vesiconaf - kcdgayaaragycaytcvatga ; vesiconar - tgngcnacdgtnardgcatt ; vesiconbf - ggnmgrttyttytchytdatgtc ; vesiconbr - tchgcngaytgcatngtytca ) on the basis of a sequence alignment of the polymerase gene of abvv and the formally classified mammalian vesiculoviruses . when the abvv - positive bat liver cdna was used as a control , the nested pcr yielded an amplicon of 704 bases , and its sequence was confirmed by cloning and sanger sequencing . future studies may use these pan - vesiculovirus pcr primers to investigate vesiculovirus diversity in other bat species and in other regions . big brown bats are prevalent in north america , where their geographic range overlaps extensively with that of humans , and considerable interactions occur between big brown bats and humans and their pets . big brown bats from this region are a known reservoir of rabies virus ; our analysis shows that these bats also constitute a sylvatic mammalian reservoir of vesiculoviruses . the other bat rhabdoviruses  mount elgon bat virus , oita rhabdovirus , and kern canyon virus  do not belong to the vesiculovirus clade but cluster together in a separate clade ( 14 ) ( figure , panel b ) . a recent report described several rhabdoviruses in oropharyngeal swab specimens from spanish bats , but the short reads ( 100 bases ) precluded a detailed phylogenetic analysis ( 15 ) . of the bats tested in our study , 5% were infected with abvv , a finding that suggests vesiculoviruses are prevalent in bats . the characterization of a novel rhabdovirus in bats with a history of human contact raises questions for further research , including health effects on the virus hosts , seroprevalence , possible transmission by insect vectors , and the extent of zoonotic exposure in humans . abvv - specific and vesiculovirus - consensus pcrs , as well as future endeavors to culture this virus , will help address these questions . detailed methods for postmortem diagnosis of rabies by direct fluorescent antibody assay in > 500 bats associated with possible human exposure submitted to the maryland department of health and mental hygiene state laboratory ; location of the primers used in this study relative to the american bat vesiculovirus ; sequences of the primers used in this study ; and bayesian inference tree based on the polymerase gene depicting relationships among the members of the family rhabdoviridae .
we identified a novel rhabdovirus , american bat vesiculovirus , from postmortem tissue samples from 120 rabies - negative big brown bats with a history of human contact . 
 five percent of the tested bats were infected with this virus . 
 the extent of zoonotic exposure and possible health effects in humans from this virus are unknown .