stoke is the second leading cause of death worldwide   as well as in korea , and thus prevention of stroke is a major public health priority . modifiable lifestyle risk factors such as dietary intake could be related to the risk of stroke . these include an inverse relationship between intake of fruits and vegetables and stroke risk [ 4 - 7 ] . meta - analyses of cohort studies show that increased intake of fruits and vegetables is associated with a reduced risk of stroke , and provides support for the recommendation to consume more than five servings of fruits and vegetables per day , which is likely to cause a major reduction in stroke . the potential protective effects of fruits and vegetables may be due to their antioxidant vitamins , folate contents , and metabolically related b vitamins such as vitamin b12 , vitamin b6 , and riboflavin . vitamin c , -carotene , and vitamin e scavenge free radicals , and vitamin c protects membranes from peroxidation by regenerating their -tocopherol components . free radical oxidation of ldl is thought to be an important contributor to the development of atherosclerosis , and thus antioxidants may slow or prevent this process and thereby decrease the risk of stroke . observational studies have shown that decreased risk of stroke is associated with increased antioxidant intake . this decreased risk is associated with some but not all antioxidant vitamins [ 11 - 13 ] , and two randomized trials found no association between supplementation with -carotene   and some other antioxidant vitamins   and a reduced risk of stroke . there has been growing interest in protection against stoke by intake of folate and related b vitamins [ 16 - 17 ] . these effects may be mediated via homocysteine , the metabolism of which requires adequate status of all four relevant b vitamins . higher folate intakes were associated with reduced stroke risk in studies of health professionals , male swedish smokers , and male finnish smokers , but not in the nurses health study . thus , the role of folate in stroke has yet to be established , and data on the intake of other b vitamins and stroke are sparse . some studies have reported an association between food group intake and stroke risk , while others assessed only selected nutrients . the purpose of the present study was to examine the hypothesis that intake of fruits and vegetables , and also their nutrients such as antioxidant vitamins and folate and related b vitamin reduces the risk of stroke after adjusting for traditional risk factors in korean population . we recruited patients admitted to hanyang university seoul hospital for treatment of first event of stroke ( n = 69 ) between may 2007 and january 2009 to participate in the present study . the 69 cases were age- , sex- , and body mass index - matched to healthy controls ( n = 69 ) . this study was approved by the institutional review board of hanyang university seoul hospital and written informed consent was obtained from all participants . anthropometric data , medical history , and socioeconomic status were obtained from both medical chart reviews and interviews . the participants ' usual dietary intakes were assessed using a semi - quantitative food frequency questionnaire ( ffq ) , which includes questions on 117 food items commonly consumed in korean meals , obtained from the korea health and nutrition examination survey ( khanes ) in 1998 . patients or caregivers were asked to indicate how often , on average , they had consumed various foods during the year prior to the interview and asked about their usual consumption patterns , so as to exclude periods of illness or dieting . the portion size was determined depending on the median value of each food determined from the 24-h recall data obtained from the khanes . for easy understanding of portion size , we provided pictures on serving size for food items . three - dimensional food models and full - scale photographs were used to assist subjects in estimating portion size . nutrient and food intake were analyzed by can - pro 3.0 ( the korean nutrition society , seoul , korea ) . blood samples were collected in edta and sst blood tubes on the day of admission , centrifuged , and then divided into aliquots for storage at -80. serum lipid profiles ( tba-30fr ; toshiba , tokyo , japan ) , blood chemicals ( coulter lh 750 , beckman coulter , inc . , fullerton , ca , usa ) , liver function ( variant ii , bio - rad , hercules , ca , usa ) , and c - reactive protein ( crp ) concentrations ( immage immunochemistry system ; beckman coulter , inc . , continuous variables were expressed using the mean and the standard errors of the mean to compare cases and controls using an independent t - test . intake of nutrients and foods was categorized into quartiles based on control values . to prevent confounding , the following covariates were included in the models : age , sex , body mass index ( bmi ; kg / m ) , family history of stroke , and energy intake ; and were selected by use of a backward variable selection method . we recruited patients admitted to hanyang university seoul hospital for treatment of first event of stroke ( n = 69 ) between may 2007 and january 2009 to participate in the present study . the 69 cases were age- , sex- , and body mass index - matched to healthy controls ( n = 69 ) . this study was approved by the institutional review board of hanyang university seoul hospital and written informed consent was obtained from all participants . anthropometric data , medical history , and socioeconomic status were obtained from both medical chart reviews and interviews . the participants ' usual dietary intakes were assessed using a semi - quantitative food frequency questionnaire ( ffq ) , which includes questions on 117 food items commonly consumed in korean meals , obtained from the korea health and nutrition examination survey ( khanes ) in 1998 . patients or caregivers were asked to indicate how often , on average , they had consumed various foods during the year prior to the interview and asked about their usual consumption patterns , so as to exclude periods of illness or dieting . the portion size was determined depending on the median value of each food determined from the 24-h recall data obtained from the khanes . for easy understanding of portion size , we provided pictures on serving size for food items . three - dimensional food models and full - scale photographs were used to assist subjects in estimating portion size . nutrient and food intake were analyzed by can - pro 3.0 ( the korean nutrition society , seoul , korea ) . blood samples were collected in edta and sst blood tubes on the day of admission , centrifuged , and then divided into aliquots for storage at -80. serum lipid profiles ( tba-30fr ; toshiba , tokyo , japan ) , blood chemicals ( coulter lh 750 , beckman coulter , inc . , fullerton , ca , usa ) , liver function ( variant ii , bio - rad , hercules , ca , usa ) , and c - reactive protein ( crp ) concentrations ( immage immunochemistry system ; beckman coulter , inc . , continuous variables were expressed using the mean and the standard errors of the mean to compare cases and controls using an independent t - test . intake of nutrients and foods was categorized into quartiles based on control values . to prevent confounding , the following covariates were included in the models : age , sex , body mass index ( bmi ; kg / m ) , family history of stroke , and energy intake ; and were selected by use of a backward variable selection method . a p value of < 0.05 was considered statistically significant . stroke cases were significantly more likely to have a family history of stroke than controls ( table 1 ) . age , sex , bmi , education level , smoking , drinking , exercise , and family history of diabetes and hypertension were similar between cases and controls . in addition , there were no significant differences in liver function , lipid profile , crp , and hemoglobin a1c . multivariable - adjusted regression analysis showed that intakes of total fat , plant fat , calcium , potassium , vitamin b1 , vitamin b2 , vitamin b6 , niacin , and folate were negatively correlated with the risk of stroke after adjusting for age , sex , bmi , and family history of stroke ( table 2 ) . although the trend was not significant , the risk of stroke was lower in the third quartile of protein intake , and the second and the third quartile of fiber intake . however , intake of antioxidant vitamins , such as carotene , vitamin c , and vitamin e was not associated with the risk of stroke . intake of vegetables was negatively associated with the risk of stroke by multivariable - adjusted regression analysis after adjusting for age , sex , bmi , and family history of stroke ( table 3 ) . the trend was not significant , but stroke risk was reduced in the second quartile of fish intake . there was no association between intake of grain , fruits , seafood , milk and meat , and the risk of stroke . consumption of vegetables and b complex vitamins , such as b1 , b2 , b6 , niacin , and folate was associated with reduced risk of stroke after adjusting for bmi , sex , age , energy intake , and family history of stroke . however , risk of stroke was not related to the intake of fruits and antioxidant vitamins . compared to subjects who ate less than four vegetable servings per day , those who ate four to six servings per day exhibited a 32% reduction in the risk of stroke , and those who ate more than six servings per day exhibited a reduction of 69% . the present study adds to the growing evidence that increased vegetable consumption is protective against stroke [ 5 - 7,18 - 19 ] , and provides support for recommendations encouraging the public to consume more than four servings of vegetables per day . the potential protective effect of vegetables on stroke is thought to be mediated through reductions in homocysteine concentration . plasma homocysteine is very responsive to intervention with b - vitamins required for its metabolism : folate , and to a lesser extent , vitamin b6 , and riboflavin . previous studies showed that intake of folate between 300 g and 821 g reduced the risk of stroke [ 17 - 19,21 - 22 ] , and the present study also observed a 90% stroke risk reduction in subjects with daily folate intake of more than 412 g . in addition , intakes of vitamin b6 , riboflavin and niacin were negatively associated with the risk of stroke in the present study . there is growing evidence that niacin inhibits vascular inflammation by decreasing endothelial reactive oxygen species production and subsequent ldl oxidation and inflammatory cytokine production , key events involved in atherogenesis . another b vitamin , vitamin b12 may also protect against stroke by mediating homocysteine metabolism , but unfortunately our database did not contain vitamin b12 content of foods . vegetables are also rich sources of potassium and calcium , which have been shown to lower blood pressure . since high blood pressure is the major cause of stroke , the effects of potassium and calcium on blood pressure may contribute to the reduced risk of stroke with an increased vegetable intake   higher dietary calcium and potassium intake are inversely correlated with the risk of stroke or stroke mortality [ 25 - 26 ] , and the systolic hypertension in the elderly study indicated that low serum potassium was associated with increased stroke incidence . randomized controlled trials have shown fruit and vegetable consumption to significantly lower systolic and diastolic blood pressure [ 28 - 29 ] , and thus possibly preventing stroke . the present study also showed a negative association between intake of calcium and potassium and stroke risk . other mechanistic effects of vegetable consumption on stroke risk may be due to antioxidant vitamins , such as vitamin c , vitamin e , and -carotene . in the iowa women 's health study   and the rotterdam study , antioxidants were shown to prevent stroke possibly by reducing lipid oxidation of ldl cholesterol [ 9 - 10 ] . however , prospective studies of nurses in the united states   and the netherlands   observed a non - significant association between the risk of stroke and intake of vitamin e , and between risk of stroke and serum concentration of vitamin e . an intervention study on the intake of vitamin c , vitamin e , and carotenoids failed to show any beneficial effect on stroke incidence or mortality . the present study showed that intake of vitamin c , vitamin e , and carotene was not associated with the risk of stroke . although these observational studies produced inconsistent results , it does not mean that there is no association between antioxidants and stroke . the results may reflect the fact that the combination of photochemicals contained in vegetables may have a greater cumulative effect than a single antioxidant . the lack of an association between intake of fruits and the risk of stroke is difficult to explain . in recent analyses from the life span study cohort , nurses ' health study   and the health professionals ' follow - up study , fruit intake was associated with a reduction in the risk of stroke . meta - analyses of nine independent cohorts reported that the average intake of fruits was two to four servings per day , and that the risk of stroke was reduced for those who ate at least three to five servings of fruit per day . however , in our study , fruit intake was low in general , with an average daily intake of one serving . sex , age , geographical origin , collection method for dietary information , subtypes of stroke , and cutoff - points of nutrient intake may influence the conclusions of various studies . the northern manhattan study reported that increased daily total fat intake , especially above 65 g , significantly increased risk of stroke , but a number of prospective cohort studies performed in japanese samples showed that higher intake of fat ( median 47 g daily ) was associated with decreased stroke risk [ 32 - 34 ] , suggesting that the very lowest levels of fat intake are associated with a higher stroke risk . the present study supported that a higher intake of fat reduced the risk of stroke in a cohort with lower levels of fat intake . the average daily intake of total fat in this study was 51 g , lower than 65 - 70 g that the united states department of health guidelines recommend for adults . several previous studies reported a significant inverse association between fish intake and risk of total stroke , particularly ischemic stroke [ 36 - 37 ] . possible mechanisms for protection against stroke by fish intake include inhibition of platelet aggregation , lowered blood viscosity , suppressed formation of leukotrienes , and reduction of plasma fibrinogen and blood pressure . in the present study , the average intake of fish was 3.5 servings per week , and although there was no significant trend , stroke risk was significantly reduced in the second quartile of fish intake ( 1.2 - 2.7 servings per week ) . our previous study showed that the average intake of n-3 fatty acids among korean adults was 1.2 g / d , lower than in eskimos but higher than american and european samples . additionally , we found that erythrocyte n-3 fatty acids could protect against hemorrhagic stroke and ischemic stroke , particularly in the case of small - artery occlusion . the food catalog included in the questionnaire was limited , and may not have captured the total intake of nutrients and food groups among our participants , which may have caused underreporting of certain food categories . our findings do not demonstrate causality since the observed relationships may be due to residual confounding from other factors associated with stroke risk , such as differences in socioeconomic status not accounted for by adjustment for clinical and environmental risk factors . in addition , the numbers of subjects included in this study was small , and the generalizability of the present data to other populations is unknown . in conclusion , our observational study suggests that intake of fat and vegetables , rich sources of vitamin b complex , calcium , and potassium may protect against stroke . larger clinical trials are necessary to confirm the causality between vegetable intake and risk of stroke , and to clarify the mechanisms through which a high intake of vegetables may protect against stroke .
consumption of vegetables and fruits is associated with a reduced risk of stroke , but it is unclear whether their protective effects are due to antioxidant vitamins or folate and metabolically related b vitamins . 
 the purpose of the study was to test the hypothesis that intake of fruits and vegetables , which are major sources of antioxidant and vitamin b complex vitamins , reduces the risk of stroke . 
 cases consisted of patients diagnosed with first event of stroke ( n = 69 ) . 
 controls ( n = 69 ) were age- , sex- , and body mass index - matched to cases . 
 multivariable - adjusted regression analysis showed that subjects who ate four to six servings of vegetable per day had a 32% reduction in the risk of stroke , and those with more than six servings per day had a reduction of 69% after adjusting for age , sex , bmi , and family history of stroke . 
 intakes of total fat , plant fat , calcium , potassium , vitamin b1 , vitamin b2 , vitamin b6 , niacin , and folate were significantly and negatively associated with the risk of stroke . although the trend was not significant , stroke risk was reduced in the second quartile ( 1.21 - 2.66 servings per week ) of fish intake . 
 however , intake of fruits ( average daily intake of 1.0 serving ) and antioxidant vitamins such as carotene , vitamin c , and vitamin e was not associated with the risk of stroke . in conclusion 
 , our observational study suggests that intake of fat and vegetables , rich sources of vitamin b complex , calcium , and potassium may protect against stroke .