we analyzed 107 isolates of t. cruzi from multiple species of free - ranging and captive wildlife , domestic animals , triatomine bug vectors , and humans who were autochthonously infected in the united states . some isolates were obtained as liquid nitrogen  stored parasites from the centers for disease control and prevention ( atlanta , ga , usa ) , the institut pasteur ( paris , france ) , and the southeastern cooperative wildlife disease study ( athens , ga , usa ) and were established in axenic liver infusion tryptose medium as described ( 9 ) . additional isolates were obtained from wild - trapped animals in axenic liver infusion tryptose medium or canine macrophage cell culture as described ( 10 ) . isolated dna was used as template for pcr amplification of 3 gene targets , mini - exon , d7 divergent domain of 24s  rrna , and 18s rrna , according to published methods ( 8) . locality data and results of molecular typing of each isolate are shown in the appendix table . all animals used in this study were cared for in accordance with the guidelines of the institutional animal care and use committee and under animal use protocol approved by the institutional animal care and use committee at the university of georgia . typical amplicon sizes of t. cruzi i and t. cruzi iia isolates from the united states are shown in the table . atypical banding patterns and isolates that differ from the standard genotype from a particular host are also represented . with the exception of human isolates , 1 primate isolate , and a few raccoon isolates , placental mammalian isolates , including those from raccoons , domestic dogs , ring - tailed lemurs , and skunks , were characterized as type iia ( appendix table ) . all remaining isolates , including those from virginia opossums ( didelphis virginiana ) , triatomine vectors , humans , and rhesus macaques from the united states , were identified as type i ( appendix table ) . because of atypical banding patterns , a clear definition of an isolate as type i vs. type iia could not be obtained . in contrast to studies conducted on south american isolates , for which 6 genotypes of t. cruzi have been identified , only 2 genotypes ( i and iia ) were identified in the current study . these data support results of investigations in central america and mexico in which a paucity of genotypes was found ( 11,12 ) . parasite specificity in regard to host species and parasite genotype ( 24 ) , although exceptions have been observed . the presence of only 2 genotypes in the united states could be caused by a lack of introduction of other genotypes or a lower diversity of natural reservoir hosts for t. cruzi than in south america . a recent analysis of t. cruzi hosts in north and south america indicated that > 48 host species representing 17 families were infected with > 1 of the 6 strains ( 4 ) . only 6 of these hosts have established populations in the united states , and us isolates from these species were only characterized as types i or iia ( 4 ) . our data for us isolates correspond with those of previous studies in which didelphis spp . are reservoirs for type i t. cruzi ( 4 ) ; no infections with type ii parasites were observed . the virginia opossum ( and its ancestors ) , which is the only marsupial present in the united states ( it migrated from south america 4.5 million years ago ) , is a possible host for t. cruzi i. this evidence suggests that t. cruzi was not recently introduced into north america or the united states ( 5 ) . additionally , sufficient time may have passed for random and rare genetic exchange events to occur independent of those found in south american isolates ( 13 ) , enabling the lineage to infect atypical reservoirs ( i.e. , raccoons ) in north america . the second major natural reservoir of t. cruzi in the united states is the raccoon . in general , the nonprimate placental mammals in our study were infected with type iia , a strain that is commonly found in sylvatic cycles in the southern cone of south america . our data confirm previous typing of us isolates by multilocus enzyme electrophoresis or random amplified polymorphic dna analysis ( 5 ) , in which 11 raccoons from georgia were characterized as zymodeme 3 ( equivalent to iia ) . although raccoons are predominately infected with t. cruzi iia , 4 known exceptions include 3 isolates from georgia and florida in the current study and 1 raccoon from louisiana from a previous study ( 5).these data are in contrast to typing data for virginia opossum isolates , which have all found t. cruzi i. this finding suggests that opossums primarily maintain persistent infections with t. cruzi i. all characterized human isolates from autochthonous us cases of infection with t. cruzi are t. cruzi i. this genotype is predominantly responsible for chagas disease north of the amazon basin and is part of the domiciliary cycle of the parasite . our findings correspond with data from mexico where t. cruzi i is the predominate strain detected in humans ( 11 ) . it would be useful to differentiate biologic characteristics and polymorphisms by using additional gene targets in human type i isolates and compare them with those in opossum , triatomine vectors , and rhesus macaque isolates from the united states . additionally , comparing these us isolates and mexican reference strains with those from south america may indicate why type i typically infects humans in north america and multiple strains are found in humans in south america . our results provide additional evidence that t. cruzi has distinct genotypes that preferentially infect 1 host species or a group of hosts . humans and marsupials are typically infected with type i t. cruzi , but raccoons , skunks , domestic dogs , and prosimians are typically infected with type iia . although we only detected t. cruzi i in triatomid bugs , other studies have detected t. cruzi iia in triatomids from the united states ( 5 ) . the mechanism is unknown by which persistent infections with a particular genotype of t. cruzi develop in certain hosts . further analysis of isolates from an increased host diversity and geographic range should be pursued . determining basic infection dynamics of reservoir hosts origin and lineage identification of 107 us isolates of trypanosoma cruzi used in 
 the study *
studies have characterized trypanosoma cruzi from parasite - endemic regions . with new human cases , increasing numbers of veterinary cases , and influx of potentially infected immigrants , understanding the ecology of this organism in the united states is imperative . 
 we used a classic typing scheme to determine the lineage of 107 isolates from various hosts .