after obtaining ethical approval from jimma university ethics review board , we collected head and body lice from persons at locations at different altitudes in ethiopia . each louse was rinsed twice in sterile water for 15 minutes , and then total genomic dna was extracted from each louse by using a qiaamp tissue kit ( qiagen , hilden , germany ) as described by the manufacturer . samples were screened by using a quantitative real - time pcr that targeted a portion of the bartonella 16s-23s intergenic spacer region ( 10 ) and a specific b. quintana gene , fabf3 , encoding 3-oxoacyl-(acyl - carrier - protein ) ( 9 ) . negative controls ( dna from noninfected lice and sterile water ) and positive controls ( dna from b. elizabethae ) were included in each assay . from 50 randomly selected persons , each of whom had at least 3 body lice and 3 head lice ; we selected 1 head louse and 1 body louse from each person for amplification and sequencing of the mitochondrial gene cytochrome b as described ( 11 ) . for phylogenetic analysis , we used mega3.1 software ( www.megasoftware.net ) . for data comparison , we used epi info version 6.0 software ( centers for disease control and prevention , atlanta , ga , usa ) . overall , we tested 271 head and 424 body adult lice collected from 134 persons ( 109 women ) . head and body lice co - infection existed on nearly all persons with a range of louse infestation of 5 to 20 lice per person . on 1 person , all but 2 persons had only black head lice ; those 2 persons each had 1 gray louse among their populations of black head lice . all black head lice belonged to genotype c , and all gray lice belonged to genotype a ( genbank accession nos . jf694384jf694442 ) . head and body lice grouped in 2 different clusters ( figure a1 ) . b. quintana was identified in 19 ( 7% ) head lice collected from 9 ( 6.7% ) patients and in 76 ( 18% ) body lice collected from 17 ( 12.7% ) patients ( table ) . no difference between male and female patients was observed in infestation with b. quintana ( p = 0.36 ) . significantly more body lice than head lice were infected with b. quintana ( p<0.001 ) . significantly more body lice were infected with b. quintana in dembi and in tum than in the other locations ( p = 0.001 and p = 0.02 , respectively ) . b. quintana in body lice was not significantly linked to altitude ( p = 0.3 with the kruskal - wallis test ) . only in the locations with the highest altitudes ( gibarku , altitude 2,395 m , and tikemit eshet , altitude 2,121 m ) did we find b. quintana in head lice ( figure ) . at these higher altitudes , we did not find b. quintana in body lice . as a result , in gibarku and in tikemit eshet , we found significantly more head lice that were infected with b. quintana than in the other locations ( p = 0.0003 and p = 0.002 , respectively ) . the presence of b. quintana in head lice was positively and significantly linked to altitude ( p = 0.014 with the kruskal - wallis test ) . our results confirm previous studies that show that genotype c head lice are prevalent in ethiopia . genotype c head lice cohabited with genotype a body lice , but we did not find an infestation of genotype a head lice in the persons studied . only 2 persons each had 1 gray louse ( genotype a ) among many black head lice ( genotype c ) . as a result , dual transmission cycles of lice appear to be occurring , and genotype c head lice may be inhibiting outbreaks of genotype a head lice . we found that 7% of the head lice and 18% of the body lice from persons from ethiopia were infected with b. quintana . for a long period , b. quintana was found only in body lice ( 12 ) . it was first identified in head lice from homeless children in nepal , where genotype c head lice exist ( 4,7 ) . additionally , b. quintana was recently found in lice from 138 homeless persons in the san francisco area ; 25% of head lice infested persons had lice pools infected with b. quintana ( 8) . however , we were unable to determine whether the study in nepal reported b. quintana infection of genotype c or genotype a head lice ( 7 ) or whether the study in san francisco reported infection of genotype a or genotype b head lice ( 8) . all persons with head lice infected with b. quintana were from locations with higher altitudes ( > 2,121 m ) , whereas at these altitudes , no body lice were infected with b. quintana . the permanent foci of body lice occur in regions subject to cold weather and in poverty - stricken areas such as ethiopia . body lice were found in 39% of ethiopian migrants , and head lice were found in 65% ( 13 ) , whereas 67% of schoolchildren from 3 different cities in ethiopia harbored body lice ( 14 ) . we do not have current hypotheses to explain the links from b. quintana to head lice at high altitudes or the mutual exclusion of b. quintana in head or body lice in the same person . in conclusion , we identified b. quintana in genotype c head lice from ethiopia only at altitudes > 2,121 m and only in patients without infected body lice . however , further epidemiologic studies with head lice collected from more patients and from different countries should be performed to determine whether this altitude dependence of b. quintana in head lice exists only in ethiopia or is a general phenomenon .
to determine the presence of bartonella quintana in head and body lice from persons in different locations in ethiopia , we used molecular methods 
 . b. quintana was found in 19 ( 7% ) genotype c head lice and in 76 ( 18% ) genotype a body lice . 
 b. quintana in head lice was positively linked to altitude ( p = 0.014 ) .