image 1organism / cell line / tissuecercospora sojinasex  sequencer or array typeillumina ga iixdata formatprocessedexperimental factorsdna extracted from a field strain , no treatmentexperimental featureswhole genome sequenceconsentn / asample source locationsoybean field from georgia , usa genome of c. sojina strain s9 , from a soybean field in georgia , was sequenced using the illumina ga iix next generation technology by paired - end sequencing method to a depth of 239   at the keck center at the university of illinois urbana - champaign . a total of 29,619,123 reads from each end were produced for a total of 59,238,246 reads from one lane . c. sojina genomes were assembled using velvet algorithm   to obtain optimized results with high quality assembly . the assembly contained 30,797,991 bases with 1804 scaffolds ( n50  =  37.690  bp ) and the g  +  c content was 53.80% . the c. sojina genes were predicted with ab initio gene finders ( fgenesh , fgenesh  + , and genewise ) . we referred to the gene models from zymoseptoria tritici ( mycosphaerella graminicola ) as the most closely related species with c. sojina to train the gene finding programs . blastx against publicly available non - redundant protein and blastn against ests databases are used to validate and curate predicted complete coding regions of the gene models . the entire dna sequence was also compared against the nonredundant protein databases in all six reading frames , using blastx with threshold e  <  1e to identify any possible coding sequences previously missed by using artemis to collate data and facilitate annotation . finally , a non - redundant set of gene models is produced , in which a single best gene model per locus is selected , preferring the candidate annotation with supporting evidence of homolog protein / est sequence in public database and complete coding sequence region . rrna genes were identified with rnammer . and trna and ncrna genes were identified by rfam and trnascan - se ,   in jgi annotation system . the complete genome sequence contained 9099 protein - coding genes , 72rrnas , 63trnas , 9ncrnas . the average size of a gene was 2742  bp and the average cds size was 1764  bp . paired - end reads59,238,246average depth - coverage of mapped reads239average read length ( bp)124assembly size ( bp)30,797,991total contigs ( >  500  bp)1804n5037,690protein - coding genes9099protein coding genes >  100 amino acids8868average cds size ( bp)1764average gene length ( bp)2742% coding64genes with known function7542genes with unknown function1557rrna72ncrna9trna63 c. sojina genome sequencing assembly and annotation statistics . all predicted genes are annotated for gene ontology ( go ) using blast2go function annotation system , , according to sequence comparison with blastp and domains / motif identification with interproscan   and pfam . the major set of genes involved in the c. sojina life cycle suggests the fundamental systems for life sustainment . additionally , the c. sojina genome contains extensive genetic factors involved in auxin biosynthetic process , and ser / thr protein kinases signal transduction and transcription factors regulation . it implies that considerable auxin regulation and signal transduction pathway involved in appropriate physiology processes and pathogenesis . abc transporters ( atp binding cassette protein ) genes were highly redundant and assigned to the largest set of molecular function category in c. sojina genome . these results accord with the report that abc transporters act as an essential virulence factors by mediating secretion of host - specific toxins compounds during pathogenesis . genome of c. sojina strain s9 , from a soybean field in georgia , was sequenced using the illumina ga iix next generation technology by paired - end sequencing method to a depth of 239   at the keck center at the university of illinois urbana - champaign . a total of 29,619,123 reads from each end were produced for a total of 59,238,246 reads from one lane . c. sojina genomes were assembled using velvet algorithm   to obtain optimized results with high quality assembly . the assembly contained 30,797,991 bases with 1804 scaffolds ( n50  =  37.690  bp ) and the g  +  c content was 53.80% . the c. sojina genes were predicted with ab initio gene finders ( fgenesh , fgenesh  + , and genewise ) . we referred to the gene models from zymoseptoria tritici ( mycosphaerella graminicola ) as the most closely related species with c. sojina to train the gene finding programs . blastx against publicly available non - redundant protein and blastn against ests databases are used to validate and curate predicted complete coding regions of the gene models . the entire dna sequence was also compared against the nonredundant protein databases in all six reading frames , using blastx with threshold e  <  1e to identify any possible coding sequences previously missed by using artemis to collate data and facilitate annotation . finally , a non - redundant set of gene models is produced , in which a single best gene model per locus is selected , preferring the candidate annotation with supporting evidence of homolog protein / est sequence in public database and complete coding sequence region . rrna genes were identified with rnammer . and trna and ncrna genes were identified by rfam and trnascan - se ,   in jgi annotation system . the complete genome sequence contained 9099 protein - coding genes , 72rrnas , 63trnas , 9ncrnas . the average size of a gene was 2742  bp and the average cds size was 1764  bp . paired - end reads59,238,246average depth - coverage of mapped reads239average read length ( bp)124assembly size ( bp)30,797,991total contigs ( >  500  bp)1804n5037,690protein - coding genes9099protein coding genes >  100 amino acids8868average cds size ( bp)1764average gene length ( bp)2742% coding64genes with known function7542genes with unknown function1557rrna72ncrna9trna63 c. sojina genome sequencing assembly and annotation statistics . all predicted genes are annotated for gene ontology ( go ) using blast2go function annotation system , , according to sequence comparison with blastp and domains / motif identification with interproscan   and pfam . the major set of genes involved in the c. sojina life cycle suggests the fundamental systems for life sustainment . additionally , the c. sojina genome contains extensive genetic factors involved in auxin biosynthetic process , and ser / thr protein kinases signal transduction and transcription factors regulation . it implies that considerable auxin regulation and signal transduction pathway involved in appropriate physiology processes and pathogenesis . abc transporters ( atp binding cassette protein ) genes were highly redundant and assigned to the largest set of molecular function category in c. sojina genome . these results accord with the report that abc transporters act as an essential virulence factors by mediating secretion of host - specific toxins compounds during pathogenesis .
fungi are the causal agents of many of the world 's most serious plant diseases causing disastrous consequences for large - scale agricultural production . 
 pathogenicity genomic basis is complex in fungi as multicellular eukaryotic pathogens . 
 the fungus cercospora sojina is a plant pathogen that threatens global soybean supplies . here , 
 we report the genome sequence of c. sojina strain s9 and detect genome features and predicted genomic elements . 
 the genome sequence of c. sojina is a valuable resource with potential in studying the fungal pathogenicity and soybean host resistance to frogeye leaf spot ( fls ) , which is caused by c. sojina . 
 the c. sojina genome sequence has been deposited and available at ddbj / embl / genbank under the project accession number ahpq00000000 .