periodontitis , an oral infectious disease , is characterized by clinical attachment loss , alveolar bone resorption , periodontal pocket formation , and gingival inflammation.1,2 one of the main objectives of periodontal therapy is regeneration of the periodontium , including restoration of the alveolar bone , cementum , and periodontal ligament lost because of periodontitis.3,4 periodontal regeneration can be achieved by guided tissue regeneration ( gtr),4 which delays the apical migration of the gingival epithelium by excluding the gingival connective tissue and allows granulation tissue derived from the periodontal ligament and osseous tissues to repopulate the space adjacent to the denuded root surface through the use of barrier membranes.5 new connective tissue attachment and bone fill , and improved clinical parameters have been documented in human biopsy6,7 and clinical gtr studies,8,9 respectively . bone grafting procedures with autogenous bone grafts , allografts , xenografts , and alloplasts are also used to promote periodontal regeneration.4 among the different available graft materials , autogenous bone remains the gold standard for osseous regeneration.4,10,11 autogenous bone has osteogenic potential , as it contains cells that participate in osteogenesis.4,12 moreover , autografts are bioabsorbable ( they are eventually replaced by the patient s own bone),10 nonallergenic ( they cause minimal tissue reaction without an immunological reaction),4,10 easy to handle , and not costly.13 rapid revascularization occurs around autogenous bone graft particles , and the graft can release growth and differentiation factors.4,14 although autogenous bone grafts present some disadvantages , such as the need for secondary surgical sites and resulting additional surgical morbidity,10,15 they can be minimized by using intraoral harvested bone.15 the use of the latter graft material is however limited by the restricted donor sites in the oral cavity for extensive grafting.4,15 in order to support barrier membranes , prevent collapse , and promote bone formation , gtr has often been combined with the placement of bone grafts or bone graft substitutes . the effectiveness of the combined procedure for treating periodontal intraosseous defects has been evaluated in comparison with the use of gtr alone in many studies , which have shown contradictory results.1619 some clinical studies have demonstrated better clinical results and bone fill with the combined procedure,16,19 whereas no significant difference was found between the treatments in other studies.17,18 moreover , few experimental studies have reported successful alveolar ridge augmentation by combining autogenous mandibular bone grafts with nonresorbable and resorbable gtr membranes.20,21 one clinical study has shown that the combination of an autogenous bone graft and a bioabsorbable gtr membrane is effective for treating three - wall periodontal defects.22 data from both clinical and histological studies suggest that periodontal regeneration occurs following treatment with autogenous bone grafts.2325 however , a 12-month clinical study has shown that autogenous cancellous bone from the jaw compared with open flap debridement is not suitable for treating intrabony periodontal defects.26 note - worthily , an autogenous cortical bone ( acb ) graft , sourced from the surgical site adjacent to the intraosseous defect , is advantageous as it prevents the need for a second surgical site while treating intraosseous periodontal defects . further , the use of a physical barrier in addition to an acb graft may enhance the regenerative outcome . the aim of this clinical trial was to evaluate the additional benefit of using gtr in conjunction with acb grafting versus acb grafting alone for the regenerative treatment of intraosseous periodontal defects . two different approaches to treat intraosseous periodontal defects were compared by using a split - mouth , randomized , controlled design . the same surgical and grafting procedures were performed in both groups of patients ; the application of a bioabsorbable gtr membrane was the only difference between the groups . the clinical and radiographic outcomes were measured on the day of surgery and at 6 months postoperatively . patients with chronic periodontitis exhibiting radiographic evidence of bone loss were recruited for the study . for inclusion , the subjects had to have similar interproximal osseous defects without furcation involvement in each of the contralateral quadrants including the premolars and molars . third molars with grade iii mobility , teeth with inadequate endodontic treatment , and/or restored teeth were excluded from the study . the exclusion criteria were systemic disease ( i.e. , diabetes mellitus , cancer , hiv , bone metabolic diseases , or disorders that compromise wound healing ) , chronic high - dose steroid therapy , radiation or immunosuppressive therapy , pregnancy , lactation , allergy or sensitivity to any drug , and smoking . the subjects had no history of drug therapy for the last 6 months before recruitment to the study . the study protocol and consent forms were approved by the university institutional review board ( assignment protocol 06.12.2005/327 ) . the initial periodontal therapy in all the patients consisted of oral hygiene instruction , full - mouth scaling and root planing , and occlusal adjustment when indicated . four to six weeks following the completion of this therapy , a periodontal reevaluation was performed to determine the patient s response to the therapy and confirm the need for periodontal surgery . furthermore , the following selection criteria had to be met : 1 ) probing pocket depth ( ppd )  6 mm ; 2 ) radiographic and intrasurgical osseous defect depth  4 mm ; 3 ) two or three osseous walls . the ppd and clinical attachment level ( cal ) were measured and plaque index ( pi)27 and gingival index ( gi)28 scores were recorded immediately before the surgery and at 6 months postoperatively by using a florida probe ( florida probe corp . , gainesville , fl , usa ) . the measurements were recorded by the same examiner ( intra - examiner calibration ) , who was blinded to the treatments , in six areas per tooth : mesiobuccal , distobuccal , midbuccal , mesiolingual , distolingual , and midlingual . standardized radiographs were obtained by using the parallel technique with a customized film holder prior to the surgery and at 6 months postoperatively.29 the radiographic defect angle was measured between the two lines representing the root surface of the involved tooth and the bone defect surface.30,31 the linear alveolar bone level , between the radiographic cementoenamel junction ( cej ) and the most apical alveolar bone , was determined by using millimeter - scale paper.29 all the operative procedures were performed on an outpatient basis under aseptic conditions by two experienced periodontal clinicians under local anesthesia ; one of them performed the surgical procedure and the other assisted during the procedure . following buccal and lingual intracrevicular incisions , full - thickness mucoperiosteal flaps were raised to allow access to the intrabony defect and facilitate primary closure where possible . vertical releasing incisions were made only if necessary for better access or to achieve better closure of the surgical site . all the granulation tissues were removed from the defects , and the roots were thoroughly scaled and planed by using hand and ultrasonic instruments . the depth of the intrabony defect was determined as the distance from the alveolar bone crest to the bottom of the defect , which is calculated as the distance between the cej and the bottom of the osseous defect minus the distance between the cej and the most coronal extension of the alveolar bone crest.32 an adequate amount of particulate cortical bone was harvested from the buccal cortical plate adjacent to the intraosseous defect by using a bone scraper and implanted into the intrabony defect . for the treatment with acb grafting and gtr , atrisorb ( atrix laboratories , inc . , fort collins , co , usa ) , an absorbable polylactide membrane , the flaps were repositioned and secured with 4 - 0 silk suture material by using the interrupted and vertical mattress suturing technique . the patients were prescribed amoxicillin + clavulanic acid ( 2 g / day for 7 days ) , flurbiprofen ( 200 mg / day for 3 days ) , and 0.2% chlorhexidine gluconate mouth rinse ( twice a day for 6 weeks ) . mechanical tooth cleaning was not allowed in the surgical area for the first 6 postoperative weeks . recall appointments for supragingival professional tooth cleaning and oral hygiene reinforcement were scheduled every other week during the first two months after surgery and once a month for the rest of the study period . a commercially available software program ( spss version 13.0 ; spss , inc . , chicago , il , usa ) was used for statistical analysis . the shapiro wilk test was used to investigate whether the data were normally distributed or not and the wilcoxon signed - rank test was used for intragroup and intergroup comparisons . the power analysis indicated that 12 defects in each treatment modality would be sufficient to demonstrate statistical significance at the p<.05 level with a power of ( at least ) 80% . two different approaches to treat intraosseous periodontal defects were compared by using a split - mouth , randomized , controlled design . the same surgical and grafting procedures were performed in both groups of patients ; the application of a bioabsorbable gtr membrane was the only difference between the groups . the clinical and radiographic outcomes were measured on the day of surgery and at 6 months postoperatively . patients with chronic periodontitis exhibiting radiographic evidence of bone loss were recruited for the study . for inclusion , the subjects had to have similar interproximal osseous defects without furcation involvement in each of the contralateral quadrants including the premolars and molars . third molars with grade iii mobility , teeth with inadequate endodontic treatment , and/or restored teeth were excluded from the study . the exclusion criteria were systemic disease ( i.e. , diabetes mellitus , cancer , hiv , bone metabolic diseases , or disorders that compromise wound healing ) , chronic high - dose steroid therapy , radiation or immunosuppressive therapy , pregnancy , lactation , allergy or sensitivity to any drug , and smoking . the subjects had no history of drug therapy for the last 6 months before recruitment to the study . the study protocol and consent forms were approved by the university institutional review board ( assignment protocol 06.12.2005/327 ) . the initial periodontal therapy in all the patients consisted of oral hygiene instruction , full - mouth scaling and root planing , and occlusal adjustment when indicated . four to six weeks following the completion of this therapy , a periodontal reevaluation was performed to determine the patient s response to the therapy and confirm the need for periodontal surgery . furthermore , the following selection criteria had to be met : 1 ) probing pocket depth ( ppd )  6 mm ; 2 ) radiographic and intrasurgical osseous defect depth  4 mm ; 3 ) two or three osseous walls . the ppd and clinical attachment level ( cal ) were measured and plaque index ( pi)27 and gingival index ( gi)28 scores were recorded immediately before the surgery and at 6 months postoperatively by using a florida probe ( florida probe corp . , gainesville , fl , usa ) . the measurements were recorded by the same examiner ( intra - examiner calibration ) , who was blinded to the treatments , in six areas per tooth : mesiobuccal , distobuccal , midbuccal , mesiolingual , distolingual , and midlingual . standardized radiographs were obtained by using the parallel technique with a customized film holder prior to the surgery and at 6 months postoperatively.29 the radiographic defect angle was measured between the two lines representing the root surface of the involved tooth and the bone defect surface.30,31 the linear alveolar bone level , between the radiographic cementoenamel junction ( cej ) and the most apical alveolar bone , was determined by using millimeter - scale paper.29 all the operative procedures were performed on an outpatient basis under aseptic conditions by two experienced periodontal clinicians under local anesthesia ; one of them performed the surgical procedure and the other assisted during the procedure . following buccal and lingual intracrevicular incisions , full - thickness mucoperiosteal flaps were raised to allow access to the intrabony defect and facilitate primary closure where possible . vertical releasing incisions were made only if necessary for better access or to achieve better closure of the surgical site . all the granulation tissues were removed from the defects , and the roots were thoroughly scaled and planed by using hand and ultrasonic instruments . the surgical sites were then rinsed with sterile saline . during surgery , the depth of the intrabony defect was determined as the distance from the alveolar bone crest to the bottom of the defect , which is calculated as the distance between the cej and the bottom of the osseous defect minus the distance between the cej and the most coronal extension of the alveolar bone crest.32 an adequate amount of particulate cortical bone was harvested from the buccal cortical plate adjacent to the intraosseous defect by using a bone scraper and implanted into the intrabony defect . for the treatment with acb grafting and gtr , atrisorb ( atrix laboratories , inc . , fort collins , co , usa ) , an absorbable polylactide membrane , the flaps were repositioned and secured with 4 - 0 silk suture material by using the interrupted and vertical mattress suturing technique . the patients were prescribed amoxicillin + clavulanic acid ( 2 g / day for 7 days ) , flurbiprofen ( 200 mg / day for 3 days ) , and 0.2% chlorhexidine gluconate mouth rinse ( twice a day for 6 weeks ) . mechanical tooth cleaning was not allowed in the surgical area for the first 6 postoperative weeks . recall appointments for supragingival professional tooth cleaning and oral hygiene reinforcement were scheduled every other week during the first two months after surgery and once a month for the rest of the study period . wilk test was used to investigate whether the data were normally distributed or not and the wilcoxon signed - rank test was used for intragroup and intergroup comparisons . the power analysis indicated that 12 defects in each treatment modality would be sufficient to demonstrate statistical significance at the p<.05 level with a power of ( at least ) 80% . twelve patients ( five men and seven women ) aged 45.34.6 years ( 3852 years ) with 24 intraosseous defects were treated . the radiographic defect angles were intermediate in both the acb graft - treated group ( 30.252.14 degrees ) and acb graft with gtr - treated group ( 29.671.83 degrees ) . an analysis of the defect characteristics at the baseline revealed no significant differences between the treatment modalities ( p>.05 ) , as summarized in table 1 . the gi scores decreased at 6 months postoperatively compared with the preoperative data ( p<.01 ) , but the pi scores were not different from the preoperative values ( p>.05 ) in both groups . intergroup comparisons of the preoperative and postoperative data showed no significant differences between the groups ( p>.05 ) . intragroup comparisons showed that both treatment modalities resulted in significant changes in the postoperative measurements compared with the preoperative values ( p<.01 ) . the ppd reduced by 4.581.08 mm in the acb graft with gtr - treated group and 4.921.00 mm in the acb graft - treated group . the preoperative cal improved by 4.251.06 and 4.500.80 mm in the acb graft with gtr - treated and acb graft - treated groups , respectively . further , the gain in radiographic alveolar bone height was 5.502.24 mm in the acb graft with gtr - treated group and 5.921.83 mm in the acb graft - treated group ( figures 1 and 2 ) . no statistically significant difference in any clinical parameters was observed between the groups ( p>.05 ) , as shown in table 3 . the results of the present study showed clinical improvement in the clinical and radiographic parameters after both regenerative treatments without significant differences between the treatment modalities . this result means that the gtr protocol did not provide an additional benefit to acb grafting . reduction in the ppd and gain in the cal are the most important clinical outcomes of regenerative therapy.33 it is well documented that a gain in the cal after any type of regenerative and conventional periodontal treatment is dependent on the initial ppd ; that is , the deeper the initial ppd , the greater is the ppd reduction and clinical attachment gain.8 in addition , the depth of the intrabony defect is the determining factor for the maximal possible attachment gain.34 in this way , a deeper intrabony defect may be related to a greater gain in the cal.8 there were no differences between the treatment groups in terms of the initial ppd and intrabony defect depth . the gain in the cal might have resulted from periodontal regeneration via new attachment or healing characterized by the formation of long junctional epithelium between the new regenerated tissues and the root surface.35 histological analysis of regenerative periodontal therapy is important in addition to observing clinical and radiographic results . as has been reported,36 both radiographic interpretations and changes in cal measurements over time are reliable for assessing the outcome of intrabony defect treatments . in other words , the use of the cal and radiographic evaluation are equally indicative of the outcome of periodontal therapies . when interpreting the findings of the present study , it should also be noted that the changes in the cal are in agreement with the gain in the radiographic alveolar bone height . in addition , postsurgical healing indicated an excellent soft tissue response to both treatments with no adverse complications . radiographic changes in the alveolar bone height may also be used when a reentry procedure is not performed . in the present study , reentry surgery was not performed for ethical reasons and the probability of further alveolar bone loss.37 instead , the split - mouth design was used in the same patient to ensure that the defects were comparable and had the same healing potential . the polylactic acid barrier membrane was selected for gtr because this type of barrier is successful in regenerative periodontal therapy3840 and shows effective clinical results similar to nonabsorbable barriers.41,42 such a barrier is easily adaptable to the defect and absorbed by rate - controlled hydrolysis.40 root resorption and ankylosis have been observed in animal and human studies after the use of autogenous bone grafts.43,44 however , via an experimental study , it was suggested that autogenous bone grafts from intraoral sources do not induce ankylosis.4 in the present study , there was no evidence of acb graft - induced root resorption and ankylosis , which appears to occur at sites where bone formation takes place without regeneration of periodontal ligament.45 the acb graft used in the present study was obtained from the buccal cortical plate adjacent to the intraosseous defect . the use of an acb graft avoids the need for a second surgical region , compared with an autogenous bone graft harvested from an extraoral or intraoral ( e.g. , mandibular symphysis , maxillary tuberosity ) region . despite the increased number of clinical and experimental studies using acb grafts for periodontal regenerative therapy in the recent years,11,13,46 an acb graft is reportedly osteoconductive but not osteogenic , because only few cells can survive.11,47 in an experimental study using a dog model with surgically created class ii furcation defects , periodontal healing was similar irrespective of the use of surgical debridement alone , acb grafting , or acb grafting with a calcium sulfate barrier.11 moreover , there is evidence that the combination of an acb graft and an enamel matrix derivative in the treatment of deep periodontal intraosseous defects leads to a significant improvement in clinical parameters.13,46 the results of the present study confirm the findings of a recent randomized controlled clinical trial that evaluated the adjunctive effect of gtr using a polylactic acid barrier combined with autogenous bone grafting in the treatment of deep intrabony periodontal defects.48 autogenous bone was harvested from the chin area in this study.48 from the clinician s perspective , it is noteworthy that the ppd reduction and cal gain in both the treatment modalities are somewhat greater than those in the previous study.48 the results obtained from this clinical study might have been influenced by the defect characteristics and center and/or operator effect , which may depend on differences among the enrolled patients , technical ability , clinical organization , and experience of the clinicians or a combination of these factors.49 within the limitations of the study , both acb grafting with gtr and acb grafting alone led to similar improvements in the clinical and radiographic parameters at 6 months postoperatively , indicating that the combined approach does not provide an additional benefit over acb grafting alone in the treatment of intraosseous periodontal defects . an acb graft , which is particulate , easy to manipulate , and highly resorbable as well as does not need a second surgical site , can be selected alone for periodontal regenerative therapy . moreover , an acb graft is considered to be a useful regenerative material for patients as it is cheaper than the other bone graft materials and completely safe , and eliminates concerns about disease transmission and immunogenic reactions associated with allogeneic or xenogeneic preparations .
objectives : the aim of this clinical trial was to evaluate the additional benefit of using guided tissue regeneration ( gtr ) with autogenous cortical bone ( acb ) grafting versus acb grafting alone for the regenerative treatment of intraosseous periodontal defects.methods:via a split - mouth design , 12 patients with chronic periodontitis ( five men , seven women ; mean age , 45.34.6 years ) who had probing pocket depths ( ppds ) of 6 mm following initial periodontal therapy were randomly assigned to two treatments in contralateral areas of the dentition : a combination of acb grafting and gtr ( with a absorbable membrane of polylactic acid ) or acb grafting alone . 
 the compared parameters were preoperative and 6-month postoperative ppds , clinical attachment levels ( cals ) , and radiographic alveolar bone heights.results:both treatment modalities resulted in significant changes in the postoperative measurements from the preoperative values ( p<.01 ) . 
 the reduction in the ppds , gain in the cals , and gain in the radiographic alveolar bone heights were 4.581.08 , 4.251.06 , and 5.502.24 mm in the patients treated with acb grafting and gtr and 4.921.00 , 4.500.80 , and 5.921.83 mm in those treated with acb grafting alone , respectively . 
 the differences between the treatments were not statistically significant ( p>.05).conclusions : within the study limitations , both acb grafting with gtr and acb grafting alone lead to significant improvements in clinical and radiographic parameters at 6 months postoperatively 
 . the combined approach does not provide any additional benefit for treating intraosseous periodontal defects .