breast conservation surgery ( bcs ) , followed by radiation therapy ( rt ) to the breast , is the preferred treatment for the majority of patients with early - stage breast cancer [ 1 , 2 ] . the overwhelming majority of local recurrences after bcs occur at or near the tumor bed [ 35 ] and pathologic studies suggest that mammographically detected cancers are rarely associated with microscopic extension of tumor away from the tumor bed [ 6 , 7 ] . these observations have led to the development and application of accelerated partial breast irradiation ( apbi ) in selected patients , where irradiation of the primary tumor bed , rather than the entire breast , may be sufficient to achieve durable local control after bcs . the evidence for apbi using interstitial or balloon brachytherapy has been building with several phase ii studies showing comparable in - breast tumor recurrence ( ibtr ) rates , as compared to ebrt techniques with better cosmetic outcomes [ 814 ] . although diverse techniques to deliver apbi have been described , each approach requires accurate identification of target structures during the planning and prescription of therapy , as all subsequent dose delivery is based on these initial volumes . seroma , an abnormal collection of serous fluid in the dead space of the lumpectomy cavity following breast - conserving surgery , is the most common post surgical complication with reported rates of 15 - 76% [ 1618 ] . the pathophysiology and risk factors for seroma formation are not fully understood , but it generally occurs in the acute postoperative period . seroma , hematoma and/or air may therefore collect in the balloon - tissue interface following placement of a balloon brachytherapy catheter and during a course of fractionated high - dose - rate ( hdr ) therapy . if not addressed , inter - fraction seroma development may ultimately interfere with dose delivery to the lumpectomy target tissue , unless the patient is imaged prior to each treatment and the dosimetry corrected . in this study , we endeavor to quantify the possible impact of seroma accumulation using dosimetric modeling in patients treated with contura ( senorx , inc . , irvine , ca ) multilumen balloon ( mlb ) brachytherapy . this is a dosimetric re - planning study using two volumes ( 30 cc and 45 cc ) in a contura mlb catheter model . the contura balloon catheter was used for this study , because it is the only fda - approved balloon brachytherapy device currently on the market that contains a vacuum port specifically designed to aspirate seroma , hematoma and/or air collection . proof of principle of aspiration of the accumulated seroma via the vacuum port is shown in fig . 1 . pre ( upper row ) and post ( lower row ) seroma aspiration images at time of ct - simulation . note improved balloon to tissue adherence two customized optimal treatment plans ( for balloon volumes of 30 and 45 cc ) were first generated using a single patient scan . uniform balloon expansion of 1 mm increments around the balloon surface was then performed to simulate a theoretical and symmetrical  virtual seroma  ( vs ) accumulation up to 9 mm ( fig . the volume ( in cc ) of this expansion was calculated by the eclipse brachyvision ( varian inc . , palo alto , ca ) software to estimate each vs. with the use of the multiple ( up to 5 ) channels of the contura catheter , the plans were generated prescribing 3.4 gy per fraction to a  ptv_eval  ( a 10 mm ptv with appropriate modifications for skin and chest wall per rtog 0413 guidelines ) . model of virtual seroma ( vs ) accumulation used for calculations new individualized plans were then generated for each 1 mm increment , representing additional accumulating vs , resulting in 10 plans for each balloon volume . each potential plan scenario and dose - volume histograms were individually generated to compare changes in dosimetric coverage of the initially defined ptv_eval . ptv_eval dosimetry was then analyzed independently for each plan to assess the effect of the vs on the percent volume of ptv_eval covered by the 100% isodose line ( idl ) ( v100 ) and the percent volume of ptv_eval covered by the 90% idl ( v90 ) . a total of twenty individual and customized 3-dimensional brachytherapy plans were generated for this comparative dosimetric study ( ten each for 30 cc and 45 cc balloon volumes ) , representing vs volumes of 6 - 60 cc . for each simulated plan , the accumulation of vs volume resulted in increased distance separating peri - cavity breast tissue target from the balloon surface . this separation produced a commensurate decrease in the v100 and the v90 of the ptv_eval ( as defined by the original treatment plan ) with increasing vs accumulation . table 1 shows the effect of increasing seroma accumulation on the plans generated for 30 cc and 45 cc balloon , respectively . each increasing uniform expansion of the balloon catheter to simulate vs resulted in a symmetrical volumetric target separation . tabular data is represented as a scatter plot for 30 cc and 45 cc balloon catheters in figs . 3 , 4 and 5 , 6 , respectively . the best - fit - line model for 30 cc balloon showed that v100 coverage of the ptv_eval decreased by 1.4% for every 1 cc accumulation of vs ( range = 7 - 10% for every 5 - 7 cc ) . a similar pattern was noted for the scatter plot analysis for 45 cc catheter , where v100 was shown to decrease by 1.3% for every 1 cc accumulation of vs ( range = 6 - 9% for every 5 - 7 cc ) . for v90 , scatter plot analyses revealed a decreased coverage of 0.9% for every 1 cc of vs for 30 cc balloon plans and 1.15% for 45 cc balloon plans . scatter plot and best fit line of v100 for repeat plans with increasing seroma volumes for 30 cc balloon catheter scatter plot and best fit line of v90 for repeat plans with increasing seroma volumes for 30 cc balloon catheter scatter plot and best fit line of v100 for repeat plans with increasing seroma volumes for 45 cc balloon catheter scatter plot and best fit line of v90 for repeat plans with increasing seroma volumes for 45 cc balloon catheter twenty plans based on 30 and 45 cc balloon catheters note the effect of accumulating seroma causing an increase in balloon to breast tissue distance . this in turn affects the volume and percentage of the initial ptv_eval covered by 100% ( v100 ) and 90% ( v90 ) idl leading to sub - optimal plans . seroma is formed by acute inflammatory exudates in response to surgical trauma and acute phase of wound healing . although the pathophysiology of seroma is incompletely understood , the formation seems to be correlated with certain cytokine levels and body mass index [ 11 , 19 ] . with regards to breast brachytherapy , post - treatment seroma formation has been shown to be more likely to result from  open soran et al .   showed a 20% rate of persistent seroma ( > 6 months ) with open placement versus 0% for post - operative placement . watkins et al .   showed that the only factor identified as statistically significant for the development of any seroma was catheter placement on a day of resection vs.  1 day later ( 59% vs. 33% ) . numerous recent imaging studies have shown that the lumpectomy cavity volume changes significantly in time frames relevant to radiotherapy planning and delivery [ 16 , 17 , 2227 ] . the range of average volume changes reported in these studies was 22.5 - 64% [ 16 , 27 ] . the change in volume of the seroma has been shown to affect the rt boost volumes with ebrt [ 22 , 25 ] . investigated the inter - fractional dose variation that occurs during ebrt when a seroma is drained during treatment . they found that the resultant change in anatomy after seroma drainage led to significant effects on dose homogeneity in the treatment volume , up to 13.9% for conventional treatment and 20.7% for imrt treatment . data suggests that the immediate postoperative course was the time during which the greatest and most rapid change occurs within the tumor bed . the volume of seroma formation is highest in the first 8 weeks following surgery , the usual time frame when breast brachytherapy is delivered . a study by kader et al .   showed that the mean seroma volume decreased from 47 cm3 in weeks 3 - 4 to 30 cm3 during weeks 7 - 8 after surgery . the purpose of our study was to quantify the potential effect of inter - fraction seroma collection around the balloon surface on the dosimetry of the lumpectomy cavity target volume ( ptv_eval ) . our analysis shows a concerning trend for sub - optimal coverage of target tissue with inter - fraction seroma accumulation , which may be under appreciated in routine clinical practice . with the potential for under dosing of tissue at highest risk for recurrence , seroma accumulation is a critical factor that warrants consideration throughout the entire process of brachytherapy treatment planning and delivery . one practical limitation of our study is that in a clinical setting , the seroma collection tends to be asymmetrical around the balloon catheter . this study results may not thus directly apply to current clinical practice , but adds credence to the hypothesis regardless , since the concept utilized is a spherical volume model . the effect on a dose volume histogram would not change if the absolute volume was symmetric or asymmetric . increased seroma formation would most likely lead to increased lubrication of the cavity and separation of the balloon from cavity walls , as well as the rotation of the balloon in the cavity . even if aspirated and the device is fixed in the cavity , it is important to verify that it has not rotated from the planned position , especially if there is an asymmetric loading . this can be easily achieved by marking the balloon catheter and the skin adjacent to it and checking the alignment of the two marks prior to each treatment . aspiration before therapy could eliminate the need for pre - treatment repeat ct - imaging and re - planning in the sub - group of patients with excessive seroma accumulation . however , an ultrasound must be performed to verify balloon integrity prior to each fraction . accumulation of seroma , hematoma or air between hdr fractions can have a significant negative impact on ptv_eval dosimetry ( v100 & v90 ) and worsen balloon catheter - tissue interface . our study suggests that inter - fraction aspiration of accumulated seroma during a course of fractionated high - dose - rate balloon brachytherapy may decrease variability in target dosimetry while also offering better fidelity to the initial ct - based treatment plan . however , not all seroma can be expected to be removed by vacuum - port aspiration . in such instances , dose - shaping with a multi - lumen catheter has the potential to provide superior coverage of the target breast tissue . parts of this work were presented as a  poster discussion  at the annual meeting of the american brachytherapy society ( abs ) held in atlanta , usa .
purposeballoon brachytherapy is a widely accepted modality for delivery of accelerated partial breast irradiation ( apbi ) . 
 our hypothesis was that inter - fraction seroma collection around the balloon surface would have an adverse effect on dosimetry of the target.material and methodsthis is a dosimetric re - planning study using two volumetric models ( 30 cc and 45 cc ) in a contura multi - lumen balloon ( mlb ) catheter . in a previously treated patient , 
 two customized baseline plans were generated using multiple channels of the contura catheter prescribed to the planning target volume evaluation ( ptv_eval ) . 
 symmetric expansions of 1.0 mm ( 0 - 9 mm ) increments around the balloon surface were performed to simulate a  virtual seroma  ( vs ) accumulation for both balloon volumes and plans were obtained for each expansion using eclipse brachyvision. an analysis of these plans was then performed to evaluate the effect of seroma accumulation on dosimetric parameters of v100 and v90.results20 plans were generated and analyzed ( 10 plans for each balloon volume ) , representing vs of 6.0 - 66.0 cc . there was a commensurate decrease in the dose delivered to the ptv_eval v100 and v90 ( as defined by the original treatment plan ) with increasing vs accumulation leading to a sub - optimal coverage of the ptv_eval . for 30 cc mlb catheter , 
 v100 decreased by 1.4% and v90 decreased by 0.9% for every 1 cc of vs. for 45cc mlb catheter , v100 decreased by 1.3% and v90 decreased by 1.15% for every 1.0 cc accumulation of vs.conclusionsballoon catheter - tissue adherence ensures daily dose delivery to the planned ptv_eval . 
 accumulation of seroma , hematoma or air between hdr fractions can significantly impact ptv_eval dosimetry . 
 vacuum - port aspiration prior to delivery of each fraction , if available , should be considered to minimize the risk of geographic under dosing .