world health organization ( who ) and the national institute on ageing ( nia ) reported that tooth loss could be a risk for alzheimer 's disease.1234 the basal ganglia , limbic system , thalamus , and cerebral cortex control the mastication and are linked with the centers of mastication , deglutition , and respiration.56 inhibition of masticatory movement linked with the mastication center and deglutition center can cause a sudden deterioration of brain function.789 masticatory efficacy could be improved by several ways in edentulous patients , from conventional complete denture to implant supported prosthesis.1011 the effect of complete denture on restoring patients ' mastication has been sufficiently evaluated by different methods , but the role of balanced complete dentures in the functional improvement of brain function has not been elucidated . this pilot study aims to know complete denture 's influence on the brain and cognitive functions in edentulous patients . the protocol of the study was approved by the institutional ethical committee of srm university , chennai , india ( srmu / m & hs / srmdc/2012/mds - pg student /205 ) . the patients of age group between 50 and 60 years were informed of the procedure and their consents were obtained ( fig . the patients selected were edentulous for a year and they were treated with complete denture for the first time . a comprehensive examination excluded patients with symptoms of temporomandibular disorders , xerostomia , orofacial motor disorders , severe oral manifestations of systemic diseases , and psychological or psychiatric conditions that could influence their response to treatment . the electroencephalogram ( eeg ) signals were used to check the brain activity.12 patients were instructed to take adequate sleep the night before and were restricted from consuming caffeine at least eight hours before the eeg analysis . the electrode placement for eeg was done in accordance to the international federation in electroencephalography and clinical neurophysiology as a 10 - 20 electrode placement system.1314 eeg recordings were obtained in two settings : before chewing paraffin gum ( saliva - check kit , gc corporation , japan ) and after three minutes of chewing gum with one - minute resting intervals between each eeg recording . the signals were sampled at 256 samples per second with 16-bit resolution and with fifteen minutes of digitized signal duration . this data was saved to be analyzed and compared with the eeg records two months after denture placement ( fig . after two months of denture usage , the eeg recordings made before and after chewing were similar to pre - treatment eeg results . both the pre - treatment and post - treatment eeg data were analyzed for differences in power spectral density ( psd ) with gum chewing . the data was pre - processed by computing average spectrum with 50% overlap in the epoch signal . eeg signal baseline wanders were viewed in the lab view platform ( national instrument , india ) . the recordings were corrected for artifacts and the signal amplitude was quantified to micro - volts.15 a digital low pass finite element filter ( fir ) filtered the eeg signal by hamming window technique . the order of the filter was 40 and the cut off frequency was 32 hz . flatness without a ripple in the pass band was desirable in the analysis of eeg signals . the filtered eeg segments were chosen for analysis . averaged spectrum and power measure were calculated . the power spectrum of the signal was computed using fast fourier transformation ( fft ) . the equation for fft is given as a formula16 : xk=k=1n-1xnwnkn : k=0, .... ,n-1 where one value of ' k ' has n complex multiplications , since ' k ' = 0 , 1 ,  , n-1 . the multiplication of x(n ) and w was done for n times , since n = 0 to n-1 . a spectral analysis of various signals measured from a patient , such as electrocardiogram ( ecg ) or electroencephalogram ( eeg ) signal , provides useful information for diagnosis . a random signal usually has finite average power and is characterized by an average power spectral density as in equation : where f ( w ) xt represents the fft output and t is the total duration of the input signal ( fig . the power spectral density of alpha waves calculated from the data was obtained ( table 1 ) . the kolmogorov - smirnov test was used to check the normal distribution of the values and the results were statistically analyzed using a paired t - test . the mean values were grouped as edentulous phase before chewing ( eeg p1 - 0.006 ) , edentulous phase after chewing ( eeg p2 - 0.007 ) , post denture insertion adaptive phase before chewing ( eeg p3 - 0.007 ) , and post denture insertion adaptive phase after chewing ( eeg p4 - 0.009 ) . the values were statistically analyzed using a paired t - test , which showed a significant increase in alpha waves psd values after chewing in both edentulous and post denture adaptive phase . the alpha waves psd related to before - chewing values increased in post denture adaptive phase ( p < .05 ) . the sensory information transmitted through the trigeminal nerve from the masticatory muscles , temporomandibular joint , and residual mucous membrane increased when subjects chewed . this transmission was unique and different from the control mechanisms involved in the movements of the arms and legs . narita et al.17 and hirano et al.18 estimated that gum chewing was more effective in stimulating brain activity than other treatments . yamazaki et al.19 demonstrated that , in both mature and old animals , the spatial memory declined as the number of hippocampal neurons decreased from impairment in hippocampal function due to early tooth loss . hippocampal dysfunction caused impairment in episodic memory such as learning new information and retrieving information.20 like hippocampus , other brain areas , such as the prefrontal cortex ( pfc ) , the striatum , and the cerebellum , play an important role in executive abilities like performing multiple tasks simultaneously , set - shifting , and inhibition . these areas are sensitive to ageing.2122 morokuma23 and klimesch24 showed a positive relationship between alpha waves and cognitive performance as well as the speed of processing information . with this concept , brain activity was evaluated by studying power spectral density of alpha waves in edentulous patients before and after denture insertion . psd values were calculated and a comparison was made for the eeg taken in two phases - edentulous phase and post denture insertion adaptive phase - before and after chewing paraffin gum . the power spectral density of alpha waves increased in both edentulous phase and post denture adaptive phase after chewing . this mechanism is believed to involve a wide area of the brain and gum chewing is thought to be effective in stimulating the brain activity.25 the study result did not synchronize with the studies performed by masumoto et al.,26 which stated no significant differences in eeg frequencies between a control and post gum condition . masumoto et al.27 contradicted their earlier study with the findings indicating that chewing flavorless and odorless gum base led to increased alpha and theta activity . comparison of psd values before chewing between edentulous phase and post denture insertion adaption phase showed that post denture insertion adaptive phase psd value increased and influenced the brain function . the limitation of this study is that the brain function can potentially be affected by other factors involving the subject , including his / her living environment , hospital visits , conversations with the attending physician , or the treatment administered . there is also a concern over the potential effect of circadian variability of eeg on the measurement sequence of brain function . within the limitations of the study , it can be concluded that the brain activity of edentulous patients improved with mastication and denture reconstruction .
purposethis pilot study was to find the influence of complete denture on the brain activity and cognitive function of edentulous patients measured through electroencephalogram ( eeg ) signals.materials and methodsthe study recruited 20 patients aged from 50 to 60 years requiring complete dentures with inclusion and exclusion criteria . 
 the brain function and cognitive function were analyzed with a mental state questionnaire and a 15-minute analysis of power spectral density of eeg alpha waves . 
 the analysis included edentulous phase and post denture insertion adaptive phase , each done before and after chewing . 
 the results obtained were statistically evaluated.resultspower spectral density ( psd ) values increased from edentulous phase to post denture insertion adaption phase . 
 the data were grouped as edentulous phase before chewing ( eeg p1 - 0.0064 ) , edentulous phase after chewing ( eeg p2 - 0.0073 ) , post denture insertion adaptive phase before chewing ( eeg p3 - 0.0077 ) , and post denture insertion adaptive phase after chewing ( eeg p4 - 0.0096 ) . 
 the acquired values were statistically analyzed using paired t - test , which showed statistically significant results ( p<.05).conclusionthis pilot study showed functional improvement in brain function of edentulous patients with complete dentures rehabilitation .