prostate cancer is the second leading cause of cancer death and the most common cancer detected in men in the united states . an estimated 217,730 new cases of prostate cancer were diagnosed in the united states , and approximately 32,050 men died of prostate cancer during 2010 . radical prostatectomy ( rp ) aims for complete cancer resection and has been shown to improve cancer survival . robotic - assisted laparoscopic prostatectomy ( ralp ) has recently emerged as an alternative to open and laparoscopic procedures . the davinci surgical system ( intuitive surgical , sunnyvale , ca ) provides 3-d visualization , higher magnification , hand tremor elimination and refined dexterity by incorporating wristed instrumentation . from 250 robotic cases in the beginning ( 2001 ) , the number has reached 73,000 in 2009 ( 86% of the 85,000 american men who had prostate cancer surgery ) . initial experiences with the davinci surgical system have been positive : short learning curve , limited blood loss , less post - operative pain , favorable complication rates , and short hospital stay [ 310 ] . despite fewer perioperative complications and shorter hospital stay , a recent paper found patients were three times more likely to require salvage therapy . one theoretical disadvantage with regards to robotic surgery is the lack of tactile feedback . in open rps , the surgeon uses his fingers to feel the periphery of the prostate gland . without tactile feedback , a robotic surgeon is unable to appreciate differences in tissue texture or firmness and therefore may not be able to tailor precisely the extent of tissue excision around the prostate gland in efforts to eradicate all cancerous tissue . inadvertently leaving residual cancer cells behind , called a positive surgical margin ( psm ) , is highly associated with cancer recurrence . psm rates were initially higher in ralp than in the open procedure , but they have been shown to decrease with surgeon s experience and improved technique . as manual palpation helps guide the surgeon in the open procedure , an equivalent real - time guiding tool is needed for robotic prostatectomy . imaging modalities like mri or ct are not feasible intraoperatively , nor do they possess the sensitivity or specificity for accurate detection and localization of prostate cancer . transrectal ultrasound ( trus ) is routinely used in diagnosis , in conjunction with digital rectal examination ( dre ) and biopsies . one center used trus for real - time monitoring and guidance during laparoscopic rp and reported technical feasibility and enhanced precision by decreased psm rates . the authors recognized however , the limitations of trus guidance ; it requires considerable prior expertise and tends to identify primarily hypoechoic lesions , which were just 47% of the cancer nodules studied . today s prostate cancer patients are more likely to present with echogenic or isoechoic lesions because aggressive screening techniques lead to a shift toward smaller , early - stage cancers ; classic b - mode gray - scale ultrasound alone can not identify these lesions . ultrasound ( us ) elastography ( use ) is emerging as a valuable tool in the field of imaging . elastography is a qualitative technique based on the principle that tissue compression produces strain ( displacement ) within that tissue ; strain is smaller in harder , stiffer tissue than in softer , more compliant tissue . analyzing the ultrasound raw radio frequency signal results in a strain map , commonly called elastogram , where harder tissue is darker than surrounding soft tissue . echogenicity and stiffness of tissue are generally uncorrelated ; use can identify hypoechoic lesions , but also echogenic or isoechoic cancers that classic gray - scale ultrasonography can not . elastography through the transrectal approach has already been proven feasible in guiding biopsies of the prostate [ 1922 ] . integrating use technology with a laparoscopic ultrasound probe will give robotic and laparoscopic surgeons an important image - guidance tool , which until this point does not exist [ 2325 ] . this paper describes two experiments and results of an ongoing study evaluating the diagnostic accuracy and efficacy of using use to identify the cancerous nodules in the prostate gland . the aim of the first study was to compare the ability of subjects to detect hard tissue ( tumor surrogates ) in synthetic and ex - vivo phantoms . we attempted to mimic an or setting of open vs. robotic procedures , by asking the subjects to identify properties of the tissue using manual palpation in one arm , versus using ultrasound elastograms in the other arm . in the second study , human ex - vivo prostatectomy specimens where used to assess the accuracy of use in the identification and characterization of hard cancerous nodules . we compared the elastogram results with histopathology maps ( the gold standard ) and also to pre- and post - operative mr scans of the prostate gland in order to assess and co - localize anatomically use with the reference histopathology and mr scans . we recruited n=25 local students to asses human ability to feel hard lesions through palpation , versus elastography s ability to distinguish the same lesions . our decision to use local students instead of seasoned surgeons stemmed from the rationale that all humans are born with the sense of touch and thus have the innate sensory ability to palpate ; we were also able to recruit more subjects in order to assess inter - observer variability . participants were asked to identify lesions present in both synthetic and ex - vivo phantoms . the hypothesis of the study was that subjects could identify lesions easier on the elastograms versus using manual palpation . the phantoms mimicked the mechanical properties of prostate tissue and the acoustic scattering properties of human tissue . synthetic phantoms exhibited deeper spherical hard lesions , consistent with deeper prostatic cancerous nodules , while ex - vivo phantoms had superficial , free - form ablated lesions , more consistent with extra - capsular cancer extension . synthetic phantoms ( 322 inches ) were made from liquid plastic ( m - f manufacturing co. , inc . they were added to the plastic mix in an 1% concentration to mimic the acoustic scattering properties of human tissue ; both the lesions and the rest of the phantom appeared isoechoic under b - mode ultrasound . lesions were created by varying the mixing ratios of liquid plastic types ; the ratio between 4116s plastic softener or 7116 plastic hardener and 8116 super - soft plastic determined the final density and the elastic modulus of the lesions and the background . each phantom contained 03 harder spherical lesions ( 12 cm diameter ) colored pink for ground truth identification ( figure 1a ) . the exposed top surface was colored opaque blue , to prevent the subjects from visually identifying the lesions ( figure 1a ) . the synthetic phantoms were sliced and sectioned at study end , following axial planes parallel with the ultrasound scanning plane . the depth of each lesion was measured as the distance between the surface of the phantom and the top of the lesion itself . the final depth of lesions for these 7 ( seven ) phantoms was measured to be between 7 and 25 mm . hard lesions of various diameters were created using radio frequency ( rf ) ablation , at an average temperature of 95 degrees fahrenheit for 20 minutes . this formed a hard spherical lesion at a depth of 16 mm below the surface , which allowed for possible palpation but not the visual localization of lesions . before ablation , each tissue was placed in a small plastic container and surrounded by 150 bloom porcine gelatin ( bloom represents an unit of measure for rigidity of gelatin ) . x - ray axial scans ( projection plane parallel with the ultrasound scanning plane ) were used to localize and measure the lesions ( figure 1d ) . the tissue phantoms were sliced on the same axial plane at study end to determine the depth and extent of the ablated areas . the depth of each lesion was measured as the distance between the surface of the phantom and the top of the lesion itself . for ex - vivo lesions , the final depth of lesions , a laparoscopic ultrasound probe was used , fitted with a transducer ( gore tetrad , englewood , co ) with a center frequency of 7.5 mhz , and 128 elements ( figure 2 ) [ 2325 ] . ultrasound raw radio - frequency data was acquired using an ultrasonix us scanner ( ultrasonix medical corporation , richmond , bc , canada ) . due to the relative inexperience of our subjects , it was not possible to have the subjects perform real - time elastography . thus , to maintain consistent image quality and to minimize user dependence , elastography images were obtained in a standardized manner by one of our researchers , prior to the evaluation . elastograms were generated using the corresponding radio frequency data and our dynamic programming ( dp ) elastography algorithm . during the study , each subject reviewed 34 phantoms , each placed in a self - designed calibration container , which consisted of a 5-by-5 grid , 0.5 inches apart , labeled with numbers and letters along the two axis ( figure 1b , c ) . subjects were asked to identify by manual palpation the location based on the provided grid ( i.e. b4 or a3 ) , and also the diameter and depth of each lesion using 0.5 inches as the unit of reference . for the use arm of the study , the subjects first underwent an use training session , where they were explained the concept and shown sample elastograms . they were then presented with 34 elastograms of the phantoms and they were asked to provide the presence , size and depth of lesions given the scale of the images . the order in which the subjects completed the use and manual palpation tasks was randomized . accuracy was determined descriptively using box and whiskers plots ( figure 3a , b ) , sensitivity and specificity calculations , and root mean squared error of estimation ( rmse ) obtained from subtracting the estimated value of the measured parameter ( diameter , depth ) from the ground truth value determined from direct measurement . stata 9 ( statacorp lp , college station , tx ) was used to perform the statistical analysis , which consisted of student s t - test for comparison between the means of the rmse of both diameter and depth as estimated via manual palpation versus use . the p - values reported were generated by t - test calculations assuming unequal variances for a two - tailed test where p=0.05 . prostate cancer patients , candidates for prostatectomy , were prospectively enrolled in our study , following an informed consent approved by the institutional review board . the objective of the study was to evaluate the efficacy of using elastography to identify and precisely localize hard nodules such as seen with prostate cancer just beneath the surface of the prostate gland in the peripheral zone . in this area , cancerous lesions are at most risk of invasion beyond the confines of the prostate gland and also more likely to be cut across by a well meaning surgeon . we recruited patients who underwent both open and assisted prostatectomies given that the process of removing the gland was not a focus of our study . 1 ) pre - operative 3 tesla mri of the pelvis was performed right before the surgery procedure . 2 ) post - operative ultra high - resolution mri at 9.4 tesla was performed on the excised prostate specimen to correlate the results to in - vivo pre - operative imaging . 3 ) use was then performed on the prostate specimen by an experienced radiologist blinded to the surgeon s findings and to the pre - operative pathology report . the collected radio - frequency ( rf ) data was used offline to recreate classic b - mode grey - scale images , and also to compute elastograms showing the stiffness of the tissue scanned . pre - operative and post - operative mr scans were used for anatomical correlation with the computed elastograms . for use use scans were performed in a systematic sextant approach , similar to that used for image guided biopsies . rf data was acquired in axial planes ( from gland s base , through mid gland , to apex ) on the left and right side of the gland ( figure 4 ) . the sextant approach was necessary to ensure that the scans were in the same plane with the histopathology diagrams ( axial ) which constituted the gold standard for comparison . use coronal scans from the left to the right of the gland were also performed ; these scans were in alignment with the mr coronal scans . for the second study , use acquisition was conducted using a siemens antares us scanner ( siemens medical solutions usa , inc . ultrasound division , issaquah , wa ) with an ultrasound research interface to access raw rf data . data was acquired by manual handling using a siemens vf 10 - 5 linear array for prostate specimens . after rf data collection , elastograms were obtained using the dynamic programming ( dp ) elastography algorithm developed in our lab . each prostate specimen underwent routine pathologic processing and analysis . due to the high volume of prostatectomies performed at our institution , the routine pathological process does not result in a whole mount mapping . instead , for histopathological evaluation the prostatectomy specimens were initially sliced at every 34 mm from apex to base , according to the stanford protocol . each slice ( 6 to 10 master slices ) the slices were stained with hematoxylin - eosin and were then analyzed under a microscope by a pathologist blinded to the surgeon s findings and also to the elastography results . the localization and size of each tumor focus were documented for all step master slices on axial diagrams , with gleason score . n=10 target areas were analyzed from n=6 patients enrolled so far into the elastography analysis . histological findings served as the gold standard in determining the presence , location and size of any prostatic nodules , malignant and benign . the objective of our study was then to compare axial elastograms findings with the histological findings recorded by the pathologist ( mapping diagrams , measurements and nodule characteristics such as malignant vs. benign ) . since histopathology diagrams often specified just the maximum diameter of a lesion , coronal elastograms were used to better establish the location and extent of the identified lesions . mri images ( both axial and coronal planes ) were aligned to the elastograms and provided help with their anatomical co - registration using anatomical details such as urethra or boundaries of peripheral zone vs. central gland . use showed higher accuracy in tumor detection with a sensitivity of 84% and specificity of 71% , compared to a sensitivity of 66% and a specificity of 67% for manual palpation . at depths greater than 20 mm , diameter estimation for synthetic phantoms using manual palpation was less accurate than use ( p=0.001 ) with a root mean squared error of estimation ( rmse ) of 4.81 for manual palpation ( 95% ci between 3.83 and 5.78 ) , versus mean rmse=3.02 for use ( 95% ci between 2.57 and 3.47 ) . for ex - vivo phantoms , estimations were comparable in both manual palpation and use , at a rmse of about 11.0 mm . depth estimation for synthetic phantoms was statistically higher using manual palpation than use ( p=0.0001 ) with a mean value of the rmse of 8.81 for manual palpation ( 95% ci between 6.24 and 11.39 ) versus a mean value of the rmse=3.02 for use ( 95% ci between 2.42 and 3.63 )  figure 3 . for ex - vivo phantoms , estimations were comparable again for both manual palpation and use , at a rmse of about 1.0 mm . elastography identified n=10 lesions , 8 hard nodules in the peripheral zone , 1 hard and 1 soft nodule in the central gland ( table 2 ) . diameter measurements correlation proved difficult because of the inability to perfectly register the three investigative modalities . use and mri measurements were within on average 2.05 mm vs. 2.25 mm of the diameters measured by pathology ( standard deviation of 1.9 mm for use and 2.9 mm for mri ) . specimen # 1 presented multiple hard and soft lesions , located in the central gland of the prostate ( figure 6 ) . the ex - vivo t2-weighted coronal image from specimen mri obtained at 9.4 tesla ( figure 6c )  here in counter clock wise orientation for better visualization of the correlation between use and mri of the specimen ) shows detailed anatomy of the heterogeneous central gland with a solid benign prostatic hypertrophy nodule ( bph ) confirmed by pathology . elastography was able to detect this solid nodule despite the heterogeneity of the prostate ( figure 6b )  solid arrow , whereas the lesion was not clearly identified by gray scale ultrasound . ex - vivo t2-weighted 9.4 tesla coronal image from specimen mri also shows an additional soft cystic bph nodule ( dashed arrow ) . urethra is also visible on the elastogram , as well as mri exam ( labeled urethra ) . the prostate , submitted for histological processing in four quadrant sections per slice , was digitally realigned to reconstruct a full histological cross - section ( figure 5c ) . specimen # 1 was found with a tumor with gleason score of 8 at the prostate base , left side ( outlined ) . figure 5a shows an ultrasound b - mode image and an elastogram obtained through an axial plane at the prostate s base on the left side . the same tumor was identified by elastography ( figure 5b  dashed contour ) but is not visible on grey - scale ultrasound . for an anatomical correlation , a soft - cystic nodule anterior to cancer can be seen on b - mode image and use ( figure 5a , b the remaining 5 ( five ) prostate specimens presented with superficial lesions in the peripheral zone of the gland . use identified multiple hard malignant lesions in various locations , from the base to the apex of the prostate gland . one can notice the clear delimitations of these lesions on use ( figure 7 ) as well as the close estimations of size versus pathology and mri . we recruited n=25 local students to asses human ability to feel hard lesions through palpation , versus elastography s ability to distinguish the same lesions . our decision to use local students instead of seasoned surgeons stemmed from the rationale that all humans are born with the sense of touch and thus have the innate sensory ability to palpate ; we were also able to recruit more subjects in order to assess inter - observer variability . participants were asked to identify lesions present in both synthetic and ex - vivo phantoms . the hypothesis of the study was that subjects could identify lesions easier on the elastograms versus using manual palpation . the phantoms mimicked the mechanical properties of prostate tissue and the acoustic scattering properties of human tissue . synthetic phantoms exhibited deeper spherical hard lesions , consistent with deeper prostatic cancerous nodules , while ex - vivo phantoms had superficial , free - form ablated lesions , more consistent with extra - capsular cancer extension . synthetic phantoms ( 322 inches ) were made from liquid plastic ( m - f manufacturing co. , inc . they were added to the plastic mix in an 1% concentration to mimic the acoustic scattering properties of human tissue ; both the lesions and the rest of the phantom appeared isoechoic under b - mode ultrasound . lesions were created by varying the mixing ratios of liquid plastic types ; the ratio between 4116s plastic softener or 7116 plastic hardener and 8116 super - soft plastic determined the final density and the elastic modulus of the lesions and the background . each phantom contained 03 harder spherical lesions ( 12 cm diameter ) colored pink for ground truth identification ( figure 1a ) . the exposed top surface was colored opaque blue , to prevent the subjects from visually identifying the lesions ( figure 1a ) . the synthetic phantoms were sliced and sectioned at study end , following axial planes parallel with the ultrasound scanning plane . the depth of each lesion was measured as the distance between the surface of the phantom and the top of the lesion itself . the final depth of lesions for these 7 ( seven ) phantoms was measured to be between 7 and 25 mm . hard lesions of various diameters were created using radio frequency ( rf ) ablation , at an average temperature of 95 degrees fahrenheit for 20 minutes . this formed a hard spherical lesion at a depth of 16 mm below the surface , which allowed for possible palpation but not the visual localization of lesions . before ablation , each tissue was placed in a small plastic container and surrounded by 150 bloom porcine gelatin ( bloom represents an unit of measure for rigidity of gelatin ) . x - ray axial scans ( projection plane parallel with the ultrasound scanning plane ) were used to localize and measure the lesions ( figure 1d ) . the tissue phantoms were sliced on the same axial plane at study end to determine the depth and extent of the ablated areas . the depth of each lesion was measured as the distance between the surface of the phantom and the top of the lesion itself . for ex - vivo lesions , the final depth of lesions for the comparative study , a laparoscopic ultrasound probe was used , fitted with a transducer ( gore tetrad , englewood , co ) with a center frequency of 7.5 mhz , and 128 elements ( figure 2 ) [ 2325 ] . ultrasound raw radio - frequency data was acquired using an ultrasonix us scanner ( ultrasonix medical corporation , richmond , bc , canada ) . due to the relative inexperience of our subjects , it was not possible to have the subjects perform real - time elastography . thus , to maintain consistent image quality and to minimize user dependence , elastography images were obtained in a standardized manner by one of our researchers , prior to the evaluation . elastograms were generated using the corresponding radio frequency data and our dynamic programming ( dp ) elastography algorithm . during the study , each subject reviewed 34 phantoms , each placed in a self - designed calibration container , which consisted of a 5-by-5 grid , 0.5 inches apart , labeled with numbers and letters along the two axis ( figure 1b , c ) . subjects were asked to identify by manual palpation the location based on the provided grid ( i.e. b4 or a3 ) , and also the diameter and depth of each lesion using 0.5 inches as the unit of reference . for the use arm of the study , the subjects first underwent an use training session , where they were explained the concept and shown sample elastograms . they were then presented with 34 elastograms of the phantoms and they were asked to provide the presence , size and depth of lesions given the scale of the images . the order in which the subjects completed accuracy was determined descriptively using box and whiskers plots ( figure 3a , b ) , sensitivity and specificity calculations , and root mean squared error of estimation ( rmse ) obtained from subtracting the estimated value of the measured parameter ( diameter , depth ) from the ground truth value determined from direct measurement . stata 9 ( statacorp lp , college station , tx ) was used to perform the statistical analysis , which consisted of student s t - test for comparison between the means of the rmse of both diameter and depth as estimated via manual palpation versus use . the p - values reported were generated by t - test calculations assuming unequal variances for a two - tailed test where p=0.05 . prostate cancer patients , candidates for prostatectomy , were prospectively enrolled in our study , following an informed consent approved by the institutional review board . the objective of the study was to evaluate the efficacy of using elastography to identify and precisely localize hard nodules such as seen with prostate cancer just beneath the surface of the prostate gland in the peripheral zone . in this area , cancerous lesions are at most risk of invasion beyond the confines of the prostate gland and also more likely to be cut across by a well meaning surgeon . we recruited patients who underwent both open and assisted prostatectomies given that the process of removing the gland was not a focus of our study . 1 ) pre - operative 3 tesla mri of the pelvis was performed right before the surgery procedure . 2 ) post - operative ultra high - resolution mri at 9.4 tesla was performed on the excised prostate specimen to correlate the results to in - vivo pre - operative imaging . 3 ) use was then performed on the prostate specimen by an experienced radiologist blinded to the surgeon s findings and to the pre - operative pathology report . the collected radio - frequency ( rf ) data was used offline to recreate classic b - mode grey - scale images , and also to compute elastograms showing the stiffness of the tissue scanned . pre - operative and post - operative mr scans were used for anatomical correlation with the computed elastograms . for use use scans were performed in a systematic sextant approach , similar to that used for image guided biopsies . rf data was acquired in axial planes ( from gland s base , through mid gland , to apex ) on the left and right side of the gland ( figure 4 ) . the sextant approach was necessary to ensure that the scans were in the same plane with the histopathology diagrams ( axial ) which constituted the gold standard for comparison . use coronal scans from the left to the right of the gland were also performed ; these scans were in alignment with the mr coronal scans . for the second study , use acquisition was conducted using a siemens antares us scanner ( siemens medical solutions usa , inc . ultrasound division , issaquah , wa ) with an ultrasound research interface to access raw rf data . data was acquired by manual handling using a siemens vf 10 - 5 linear array for prostate specimens . after rf data collection , elastograms were obtained using the dynamic programming ( dp ) elastography algorithm developed in our lab . each prostate specimen underwent routine pathologic processing and analysis . due to the high volume of prostatectomies performed at our institution , the routine pathological process does not result in a whole mount mapping . instead , for histopathological evaluation the prostatectomy specimens were initially sliced at every 34 mm from apex to base , according to the stanford protocol . each slice ( 6 to 10 master slices ) were then incorporated in a paraffin block and sliced at 5  meter thickness . the slices were stained with hematoxylin - eosin and were then analyzed under a microscope by a pathologist blinded to the surgeon s findings and also to the elastography results . the localization and size of each tumor focus n=10 target areas were analyzed from n=6 patients enrolled so far into the elastography analysis . histological findings served as the gold standard in determining the presence , location and size of any prostatic nodules , malignant and benign . the objective of our study was then to compare axial elastograms findings with the histological findings recorded by the pathologist ( mapping diagrams , measurements and nodule characteristics such as malignant vs. benign ) . since histopathology diagrams often specified just the maximum diameter of a lesion , coronal elastograms were used to better establish the location and extent of the identified lesions . mri images ( both axial and coronal planes ) were aligned to the elastograms and provided help with their anatomical co - registration using anatomical details such as urethra or boundaries of peripheral zone vs. central gland . use showed higher accuracy in tumor detection with a sensitivity of 84% and specificity of 71% , compared to a sensitivity of 66% and a specificity of 67% for manual palpation . at depths greater than 20 mm , diameter estimation for synthetic phantoms using manual palpation was less accurate than use ( p=0.001 ) with a root mean squared error of estimation ( rmse ) of 4.81 for manual palpation ( 95% ci between 3.83 and 5.78 ) , versus mean rmse=3.02 for use ( 95% ci between 2.57 and 3.47 ) . for ex - vivo phantoms , estimations were comparable in both manual palpation and use , at a rmse of about 11.0 mm . depth estimation for synthetic phantoms was statistically higher using manual palpation than use ( p=0.0001 ) with a mean value of the rmse of 8.81 for manual palpation ( 95% ci between 6.24 and 11.39 ) versus a mean value of the rmse=3.02 for use ( 95% ci between 2.42 and 3.63 )  figure 3 . for ex - vivo phantoms , estimations were comparable again for both manual palpation and use , at a rmse of about 1.0 mm . elastography identified n=10 lesions , 8 hard nodules in the peripheral zone , 1 hard and 1 soft nodule in the central gland ( table 2 ) . diameter measurements correlation proved difficult because of the inability to perfectly register the three investigative modalities . use and mri measurements were within on average 2.05 mm vs. 2.25 mm of the diameters measured by pathology ( standard deviation of 1.9 mm for use and 2.9 mm for mri ) . specimen # 1 presented multiple hard and soft lesions , located in the central gland of the prostate ( figure 6 ) . the ex - vivo t2-weighted coronal image from specimen mri obtained at 9.4 tesla ( figure 6c )  here in counter clock wise orientation for better visualization of the correlation between use and mri of the specimen ) shows detailed anatomy of the heterogeneous central gland with a solid benign prostatic hypertrophy nodule ( bph ) confirmed by pathology . elastography was able to detect this solid nodule despite the heterogeneity of the prostate ( figure 6b )  solid arrow , whereas the lesion was not clearly identified by gray scale ultrasound . ex - vivo t2-weighted 9.4 tesla coronal image from specimen mri also shows an additional soft cystic bph nodule ( dashed arrow ) . urethra is also visible on the elastogram , as well as mri exam ( labeled urethra ) . the prostate , submitted for histological processing in four quadrant sections per slice , was digitally realigned to reconstruct a full histological cross - section ( figure 5c ) . specimen # 1 was found with a tumor with gleason score of 8 at the prostate base , left side ( outlined ) . figure 5a shows an ultrasound b - mode image and an elastogram obtained through an axial plane at the prostate s base on the left side . the same tumor was identified by elastography ( figure 5b  dashed contour ) but is not visible on grey - scale ultrasound . for an anatomical correlation , a soft - cystic nodule anterior to cancer can be seen on b - mode image and use ( figure 5a , b  arrows ) and on histopathology ( figure 5c  arrows ) . the remaining 5 ( five ) prostate specimens presented with superficial lesions in the peripheral zone of the gland . use identified multiple hard malignant lesions in various locations , from the base to the apex of the prostate gland . one can notice the clear delimitations of these lesions on use ( figure 7 ) as well as the close estimations of size versus pathology and mri . use showed higher accuracy in tumor detection with a sensitivity of 84% and specificity of 71% , compared to a sensitivity of 66% and a specificity of 67% for manual palpation . at depths greater than 20 mm , diameter estimation for synthetic phantoms using manual palpation was less accurate than use ( p=0.001 ) with a root mean squared error of estimation ( rmse ) of 4.81 for manual palpation ( 95% ci between 3.83 and 5.78 ) , versus mean rmse=3.02 for use ( 95% ci between 2.57 and 3.47 ) . for ex - vivo phantoms , estimations were comparable in both manual palpation and use , at a rmse of about 11.0 mm . depth estimation for synthetic phantoms was statistically higher using manual palpation than use ( p=0.0001 ) with a mean value of the rmse of 8.81 for manual palpation ( 95% ci between 6.24 and 11.39 ) versus a mean value of the rmse=3.02 for use ( 95% ci between 2.42 and 3.63 )  figure 3 . for ex - vivo phantoms , estimations were comparable again for both manual palpation and use , at a rmse of about 1.0 mm . elastography identified n=10 lesions , 8 hard nodules in the peripheral zone , 1 hard and 1 soft nodule in the central gland ( table 2 ) . diameter measurements correlation proved difficult because of the inability to perfectly register the three investigative modalities . use and mri measurements were within on average 2.05 mm vs. 2.25 mm of the diameters measured by pathology ( standard deviation of 1.9 mm for use and 2.9 mm for mri ) . specimen # 1 presented multiple hard and soft lesions , located in the central gland of the prostate ( figure 6 ) . the ex - vivo t2-weighted coronal image from specimen mri obtained at 9.4 tesla ( figure 6c )  here in counter clock wise orientation for better visualization of the correlation between use and mri of the specimen ) shows detailed anatomy of the heterogeneous central gland with a solid benign prostatic hypertrophy nodule ( bph ) confirmed by pathology . elastography was able to detect this solid nodule despite the heterogeneity of the prostate ( figure 6b )  solid arrow , whereas the lesion was not clearly identified by gray scale ultrasound . ex - vivo t2-weighted 9.4 tesla coronal image from specimen mri also shows an additional soft cystic bph nodule ( dashed arrow ) . urethra is also visible on the elastogram , as well as mri exam ( labeled urethra ) . the prostate , submitted for histological processing in four quadrant sections per slice , was digitally realigned to reconstruct a full histological cross - section ( figure 5c ) . specimen # 1 was found with a tumor with gleason score of 8 at the prostate base , left side ( outlined ) . figure 5a shows an ultrasound b - mode image and an elastogram obtained through an axial plane at the prostate s base on the left side . the same tumor was identified by elastography ( figure 5b  dashed contour ) but is not visible on grey - scale ultrasound . for an anatomical correlation , a soft - cystic nodule anterior to cancer can be seen on b - mode image and use ( figure 5a , b the remaining 5 ( five ) prostate specimens presented with superficial lesions in the peripheral zone of the gland . use identified multiple hard malignant lesions in various locations , from the base to the apex of the prostate gland . one can notice the clear delimitations of these lesions on use ( figure 7 ) as well as the close estimations of size versus pathology and mri . in surgical procedures where manual palpation would be helpful but not possible to perform , e. g. laparoscopic robotic surgery , use can offer added value if proven to be accurate in detecting pathologic lesions . our comparative study showed use to be superior to manual palpation in general sensitivity and specificity , and also in identifying deeper lesions . despite of the inexperience of our subjects with use and elastogram evaluation , use demonstrated good performance in detection of hard lesions . our feasibility study showed that use was able to identify both hard and soft lesions in the ex - vivo prostate specimens , located in the deep prostatic central gland and in the peripheral zone . histopathologic findings validated use , and results compared favorably with the in - vivo pre - surgical and ex - vivo post - surgical mri scans . in the central gland of the prostate , elastography showed excellent detection of hard and soft areas , despite the complexity of the central gland . elastography was able to identify both hard and soft bph nodules and anatomical landmarks like the urethra ( note excellent anatomical correlation to mr scan findings ) . in the peripheral zone use identified multiple hard malignant lesions , from the base to the apex of the prostate gland . these preliminary results demonstrate the ability of use to detect hard nodules in the prostate and are encouraging in the pursuit of this technology as a palpation equivalent imaging tool for prostatectomy . use maps tissue elasticity which makes it an ideal imaging modality to serve as a surrogate and possible equivalence to manual palpation in identifying hard cancerous tissue in the prostate gland , especially in the peripheral zone but also in the central gland . real - time intra - operative imaging guidance is needed for identifying the presence of cancer within the prostate , especially near the capsule where tumor can invade and spread outside of the gland , and also for studying surrounding structures . if diseased hard lymph nodes could be detected , then lymphadenectomy may provide a more accurate cancer staging , help tailor future therapy , and potentially prevent recurrence . a better delineation of the bladder neck and apex during dissection , especially when prostate cancer is located at the apex could perhaps improve patients outcome . if deemed possible , imaging cavernous nerves ( cns ) located along the immediate surface of the prostate gland may lead to their preservation , and thus improved preservation of potency and urinary continence . further more , the development of the elastography technology as an imaging guiding tool during prostatectomy could potentially be useful in the open procedures as well , where the manual palpation would not be enough to identify deeper lesions . it has been documented in the literature that prostate carcinoma originates in the central gland and transitional zone in up to 30% of cases . our initial experience showed use was able to reliably identify hard nodules in the peripheral zone of the prostate that were prostate cancers . additionally , use showed its ability to define tissue hardness of bph nodules despite the underlying tissue complexity in the central gland . our comparative study demonstrated use can approach the efficacy of manual palpation for superficial lesions and has the potential to surpass it for smaller , deeper lesions . we employed a laparoscopic ultrasound probe which was successfully used and tested in conjunction with elastography algorithms . our initial experience with use encourages us to pursue further the evaluation of this technique . we can conclude that there is promise is integrating laparoscopic ultrasound elastography as a real - time , in - vivo imaging tool to guide surgeons during robotic - assisted prostatectomies .
summarybackgroundduring laparoscopic or robotic assisted laparoscopic prostatectomy , the surgeon lacks tactile feedback which can help him tailor the size of the excision . 
 ultrasound elastography ( use ) is an emerging imaging technology which maps the stiffness of tissue . in the paper we are evaluating use as 
 a palpation equivalent tool for intraoperative image guided robotic assisted laparoscopic prostatectomy.material/methodstwo studies were performed : 1 ) a laparoscopic ultrasound probe was used in a comparative study of manual palpation versus use in detecting tumor surrogates in synthetic and ex - vivo tissue phantoms ; n=25 participants ( students ) were asked to provide the presence , size and depth of these simulated lesions , and 2 ) a standard ultrasound probe was used for the evaluation of use on ex - vivo human prostate specimens ( n=10 lesions in n=6 specimens ) to differentiate hard versus soft lesions with pathology correlation . 
 results were validated by pathology findings , and also by in - vivo and ex - vivo mr imaging correlation.resultsin the comparative study , use displayed higher accuracy and specificity in tumor detection ( sensitivity=84% , specificity=74% ) . 
 tumor diameters and depths were better estimated using use versus with manual palpation . 
 use also proved consistent in identification of lesions in ex - vivo prostate specimens ; hard and soft , malignant and benign , central and peripheral.conclusionsuse is a strong candidate for assisting surgeons by providing palpation equivalent evaluation of the tumor location , boundaries and extra - capsular extension . 
 the results encourage us to pursue further testing in the robotic laparoscopic environment .