triple - negative breast cancer represents a distinct subset of breast cancer that exhibits a more aggressive course as compared to other biological subtypes of cancer [ 17 ] . the aggressiveness of the disease is best illustrated by the fact that the peak risk of recurrence of the disease is between the first and third year following diagnosis , and that survival after recurrence is significantly shorter than that observed in patients with non - triple - negative controls [ 4 , 6 , 810 ] . surprisingly , patients who did not have a recurrence of the disease within the first 8  years after the diagnosis did not subsequently relapse . however , in other subtypes of breast cancer , the risk of recurrence keeps rising as time progresses [ 4 , 11 ] . the recurrence pattern of triple - negative breast cancer also differs from other biological subtypes of cancer . in addition , the most characteristic sites of metastases include the brain and lungs [ 3 , 6 , 12 ] . from a biological point of view , triple - negative breast cancer remains a heterogeneous group with difficult - to - define subtypes . gene expression studies have shown that er - negative and her2-negative tumors are clustered into at least three distinct molecular classes : basal - like , normal - like and claudin - negative breast cancer [ 1 , 11 , 1319 ] . most of triple - negative tumors show basal - like phenotype which can be subdivided into pure variant and myoepithelial variant . immunohistochemical ( ihc ) signatures  have been described as a surrogate of microarray , but the panel proposed by nielsen et al .   seems to be the best example of the classification to date . in this classification , basal - like cancers are defined as those lacking er and her2 expression and expressing cytokeratin 5/6 ( ck 5/6 ) and/or epidermal growth factor receptor ( egfr or her1 ) . this panel has 100% specificity and 76% sensitivity for identification of basal - like cancers . patients with triple - negative breast cancer and brain metastases have the poorest prognosis out of all biological subtypes of breast cancer after dissemination to the brain . it is not known whether such a short survival depends on clinical features ( performance status , dissemination of the disease to other organs ) , or the affiliation to the specific biological subset , which can be selected by molecular markers , for example basal cytokeratins . the aim of the present study was to analyze clinical features and survival of triple - negative breast cancer patients with brain metastases and to compare basal - like and non - basal - like subtypes in order to establish the clinical value of basal biological markers differentiating these subtypes . between 1 january 2003 and 31 december 2009 , 111 patients with triple - negative ( er - negative , pgr - negative , her2-negative ) breast cancer and brain metastases were treated in the breast cancer and reconstructive surgery department at the maria sklodowska - curie memorial cancer center , warsaw , poland . the observation of the patients started at the time of the detection of brain metastases and all data were collected prospectively in our database . in each case , treatment options were approved by a team of medical oncologists , radiation oncologist , neurologist and neurosurgeon , and were performed after patients had signed written consent . immunohistochemistry ( ihc ) was carried out in order to evaluate levels of er , pgr and her2 expression in primary breast tumors . staining was performed using primary antibodies against er ( clone 6f11 , novocastra , dilution scale 1:100 ) ; against pr ( clone 16 , novocastra , dilution scale 1:200 ) and against her2 ( polyclonal hercep test , dako ) . for the evaluation of er and pr expression , fluorescence in situ hybridization ( fish ) was used for all her2 2 + tumors using the her2 dna probe kit abbott ( vysis ) . her2-positive staining was defined as ihc 3 + or , in the case of ihc 2 + , fish - positive . her2-negativity was defined as ihc 0 , 1 + or 2 + , along with negative fish results . immunohistochemistry for cytokeratin 5/6 ( clone d5/16b4 , dako , dilution scale 1:100 ) , her1 ( egfr pharm dx , clone 2 - 18c9 , dako , rtu ) and c - kit ( cd117 , policlonal , dako , dilution scale 1:500 ) , as a surrogate of cdna microarray , was performed in 75 patients in whom paraffin blocs with primary tumor samples were available in the pathology archives . these assays were performed after the detection of brain metastases . based on the definition by nielsen et al . , tumors were divided into basal - like and non - basal - like biological subsets . in 36 out of 111 patients , the analysis of basal markers was impossible to perform for the following reasons : firstly , there was an insufficient amount of biological material to perform detailed analysis . in some of those patients , only core biopsy or fine needle biopsy was performed to confirm the initial diagnosis of breast cancer and , after detection of brain metastases , we did not have enough biological material to perform ihc analysis . secondly , some patients were treated in different hospitals before the dissemination of the disease , and after the detection of brain metastases , it was impossible to gain access to paraffin blocks from their primary tumors . thirdly , according to information provided by the remaining patients , the ihc was impossible to perform due to technical difficulties that occurred during pathological processing . descriptive statistics were used to determine patient demographics and clinical characteristics . in order to compare categorical tumor features in the basal - like and non - basal - like subsets , in which the chi - square test was inappropriate because of small sample size , the fisher exact test was used . a univariate analysis and cox proportional hazards model were developed to identify factors influencing survival after brain metastasis in patients with triple - negative breast cancer . the following factors were analyzed in a cox model : kps ( < 70 vs. 70 ) , age at detection of brain metastases ( < 50 vs. 50 ) , extracranial disease ( absent vs. present ) , extracranial disease ( controlled vs. uncontrolled ) , lung metastases ( absent vs. present ) , brain metastases as the first or the only metastatic site ( yes vs. no ) , neurosurgery ( yes vs. no ) , systemic treatment after brain metastases ( yes vs. no ) , basal subtype vs. non - basal subtype . disease - free survivals ( dfs ) , overall survivals ( os , from initial diagnosis of breast cancer ) , and survivals from the detection of brain metastases in the entire group and in biological subgroups were estimated using the kaplan  meier method and compared using the log - rank test . descriptive statistics were used to determine patient demographics and clinical characteristics . in order to compare categorical tumor features in the basal - like and non - basal - like subsets , the chi - square test was used . for those categorical variables in which the chi - square test was inappropriate because of small sample size a univariate analysis and cox proportional hazards model were developed to identify factors influencing survival after brain metastasis in patients with triple - negative breast cancer . the following factors were analyzed in a cox model : kps ( < 70 vs. 70 ) , age at detection of brain metastases ( < 50 vs. 50 ) , extracranial disease ( absent vs. present ) , extracranial disease ( controlled vs. uncontrolled ) , lung metastases ( absent vs. present ) , brain metastases as the first or the only metastatic site ( yes vs. no ) , neurosurgery ( yes vs. no ) , systemic treatment after brain metastases ( yes vs. no ) , basal subtype vs. non - basal subtype . disease - free survivals ( dfs ) , overall survivals ( os , from initial diagnosis of breast cancer ) , and survivals from the detection of brain metastases in the entire group and in biological subgroups were estimated using the kaplan  meier method and compared using the log - rank test . clinical characteristics of the entire group are presented in table  1 . in the group of 111 patients , histopathology and metastatic spread that is typical for triple - negative breast cancer was revealed . in most of the patients , ductal cancer with histological grade 3 was detected , although medullary and metaplastic cancers were also found . lungs and brain were the most relevant sites of distant metastases . among 75 patients in whom basal markers were assessed , 48 ( 64% ) tumors expressed ck5/6 , 26 ( 36% ) tumors expressed her1 and 13 ( 17% ) expressed c - kit . in 6 tumors ( 8% ) , expression of three basal biological markers was detected and 13 tumors ( 17% ) did not express any of them . , basal - like tumors were identified based on panel of four antibodies ( er , her2 , her1 and cytokeratin 5/6 ) . there were 57 patients ( 76% ) with basal - like and 18 ( 24% ) with non - basal - like biological subtypes . the comparison between basal - like and non - basal - like subsets is presented in table  2 . there were no significant differences between both groups in relation to patients age at initial diagnosis , clinical stage and tumor histologic grade ; however , medullar and metaplastic cancers were observed only in the basal - like subset . bone metastases , liver metastases and brain metastases as a first site of dissemination were all equally distributed in both groups , but lung metastases were more common in the non - basal subtype.table  1characteristics of 111 patients with triple - negative breast cancer patients with brain metastasescharacteristicno . of patients%initial tnm stage  i1816  ii4541  iii3935  iv98histological type  ductal carcinoma7870  lobular carcinoma21  medullar carcinoma33  papillar carcinoma11  mucinous carcinoma11  metaplastic carcinoma11  neuroendocrine carcinoma11cancer cells or invasive cancer after  chemotherapy2422number of brain metastases  12724  276.5  365.5  multiple7164localization of metastases  brain as the first or only site3632  liver1917  lung5449  bone2724locoregional recurrence3431neurosurgery2018systemic therapy after wbrt5953wbrt whole - brain radiation therapyin most patients , several lines of chemotherapy were usedtable  2differences in clinical features between patients with basal - like ( er / pgr / her2-negative , ck5/6-positive and/or her1-positive ) and non - basal - like ( er / pgr / her2-negative , ck5/6-negative and her1-negative ) biological subtypes of triple - negative breast cancer ( 75 patients)factorbasal - like subtype ( % ) ( er / pgr / her2-negative , ck5/6-positive and/or her1-positive)non - basal - like subtype ( % ) ( er / pgr / her2-negative , ck5/6-negative and her1-negative)p valuenumber of patients57 ( 76%)18 ( 24%)age at initial diagnosis ( years)50470.343age at brain metastases ( years)54500.495kps  < 7028 ( 49%)6 ( 33% )  7029 ( 51%)12 ( 67%)0.184initial tnm stage  i8 ( 14%)0  ii20 ( 35%)10 ( 56% )  iii23 ( 40%)7 ( 39% )  iv6 ( 11%)1 ( 5%)0.062histological type and grade  ductal carcinoma grade 330 ( 55%)10 ( 59% )  other25 ( 45%)7 ( 41%)0.49number of brain metastases  113 ( 23%)4 ( 22% )  23 ( 5%)1 ( 6% )  33 ( 5%)0  multiple38 ( 67%)13 ( 72%)1.0brain as the first / only site20 ( 35%)4 ( 22%)0.308extracranial metastases37 ( 65%)15 ( 83%)0.116lung metastases22 ( 39%)13 ( 72%)0.013liver metastases9 ( 16%)2 ( 11%)0.447bone metastases15 ( 26%)3 ( 17%)0.310loca / locoregional recurrence14 ( 25%)7 ( 39%)0.200neurosurgery of brain metastasis12 ( 21%)1 ( 6%)0.120systemic treatment after wbrt29 ( 51%)11 ( 61%)0.314c - kit9 ( 16%)4 ( 22%)0.575 characteristics of 111 patients with triple - negative breast cancer patients with brain metastases wbrt whole - brain radiation therapy in most patients , several lines of chemotherapy were used differences in clinical features between patients with basal - like ( er / pgr / her2-negative , ck5/6-positive and/or her1-positive ) and non - basal - like ( er / pgr / her2-negative , ck5/6-negative and her1-negative ) biological subtypes of triple - negative breast cancer ( 75 patients ) dfs , os and survival from brain metastases in triple - negative , basal - like and non - basal - like subsets are presented in table  3 . in the entire group of 111 triple - negative breast cancer patients with brain metastases , median dfs , os and survival from brain metastases were 20 , 29 and 4  months , respectively . in 75 patients with known basal markers , median dfs , os and survival from brain metastases were 18 , 26 and 3.2  months , respectively . in the   basal - like subtype , they were 15 , 26 and 3  months , respectively , and in the non - basal - like subtype they were 20 , 30 and 2.8  months , respectively . no statistically significant differences in dfs , os and survival from brain metastases were detected between basal - like and non - basal - like biological subtypes.table  3median survival of patients with triple - negative breast cancer ( 75 patients ) , basal - like subtype ( 57 patients ) and non - basal - like subtype ( 18 patients)no . of patientsmedian survival ( months)95% cip - valuedisease - free survival  triple - negative751813.728 ; 21.600  basal - like571510.764 ; 19.308 non - basal - like18208.892 ; 31.5600.284overall survival  triple - negative752617.172 ; 34.248  basal - like572417.292 ; 31.428  non - basal - like183019.188 ; 40.7640.227survival from brain metastases  triple - negative753.22.532 ; 3.900  basal - like573.22.904 ; 3.528  non - basal - like182.80.000 ; 5.8920.880 median survival of patients with triple - negative breast cancer ( 75 patients ) , basal - like subtype ( 57 patients ) and non - basal - like subtype ( 18 patients ) the analysis of factors influencing survival from brain metastases in triple - negative breast cancer patients with brain metastases , including clinical and biological factors was assessed in univariate and multivariate analysis . the results of univariate analysis of the group of 111 patients are presented in table  4 . the results of cox multivariate analysis ( final model ) is presented in table  5 . multivariate analysis revealed that clinical , and not biological factors influenced survival from brain metastases . age at the detection of brain metastases was a factor of borderline significance ; younger patients lived longer . none of three analyzed basal markers ( ck 5/6 , her1 , c - kit ) had any influence on survival from brain metastases.table  4factors influencing survival from brain metastases in patients with triple - negative breast cancer : univariate analysis ( 111 patients)covariatemedian time ( months)p valuekps  < 702.2  706.3<0.0001age at initial diagnosis  < 503.3  50653.9  > 653.60.692age at the detection of brain metastases  < 506.3  50653.0  > 653.60.024initial tnm stage i  +  ii3.3  iii  +  iv4.80.158extracranial disease  present3.2  absent6.30.063extracranial disease  controlled ( stable / responsive)8.2  uncontrolled ( progressive)2.8<0.0001locoregional recurrence  present4.2  absent2.80.304lung metastases  present3.1  absent6.30.045neurosurgery  yes12  no3.20.002systemic treatment after wbrt  yes4.9  no2.60.148type of chemotherapy after wbrt  with capecitabine3.1  with vinorelbine5.9  with taxanes5.4  with antracyclines9.8  with platinum salts or etoposide3.9  without chemotherapy2.60.496table  5cox multivariate analysis of factors influencing survival from brain metastases , final modelcovariatehrp value95% cikarnofsky performance status kps    700.319<0.00010.181 ; 0.563age at brain metastases > 501.6810.0590.981 ; 2.881controlled extracranial disease0.3830.0010.222 ; 0.660 factors influencing survival from brain metastases in patients with triple - negative breast cancer : univariate analysis ( 111 patients ) cox multivariate analysis of factors influencing survival from brain metastases , final model clinical characteristics of the entire group of 111 triple - negative breast cancer patients with brain metastases confirm the results of our previous studies [ 2 , 3 ] and are similar to the results of other authors [ 6 , 8 , 12 , 21 , 22 ] . due to the fact that we still do not know the biological or molecular factors that are responsible for dissemination to the brain and poor survival after the detection of brain metastases , we tried to find out if basal markers could play a major role in this processes . so we compared clinical features and survival of patients with triple - negative tumors and brain metastases which expressed and did not express basal markers . because it was not possible to perform dna microarray , we used ihc as a surrogate of molecular analysis . in our material , these results are in line with the findings of nielsen et al . , livacy et al .   and kreike et al . . her1 was expressed in 36% of tumors and it is also in agreement with the literature ; her1 was expressed in up to 66% of basal - like and triple - negative breast cancers , but her1 responsible for activating gene mutations were remarkably rare [ 16 , 21 , 2426 ] . the third basal marker , c - kit was expressed in 17% of tumors . it was less than in the study by nielsen et al .   and kreike . in our material , the proportion of patients with basal - like and non - basal - like phenotypes was 76% and 24% , respectively . this result was comparable to that observed by other authors [ 13 , 14 , 18 , 19 , 25 , 27 ] . however , in some papers , the percentage of patients with the basal - like subtype was about 50% . there are few data in the literature concerning differences between basal - like and non - basal - like subtypes of triple - negative breast cancer according to clinical features and survival . to our knowledge , the present study is the first one analyzing such relationships in patients with brain metastases . we did not observe statistically significant differences between basal - like and non - basal - like subtypes apart from the pattern of distant metastases . compared the clinicopathological and immunohistochemical features of triple - negative tumors depending on the expression of specific basal markers . the authors did not show any difference in morphological features between both subtypes , but basal - like breast cancer showed distinct immunophenotypical differences with the expression of several markers of poor prognosis . in addition , the authors observed a unique pattern of distant metastases of the basal - like subtype with more frequent metastasis to brain and lung . in the present study , in 35% of patients with the basal - like phenotype , the brain was the first site of distant metastasis . the high propensity of the basal - like phenotype to metastasize to the brain as the first site of distant recurrence is worth further molecular investigation . it is possible that some molecular markers allow anchor cancer cells into the brain ( theory of  soil and seed  ) . if we knew such a molecular marker , we would be able to select a group of breast cancer patients with high risk of brain metastases in order to take preventive or screening activities . in the present study , median dfs of all patients with triple - negative breast cancer with brain metastases was less than 2  years ( 20  months ) and os was less than 3  years ( 29  months ) . in which median dfs of patients with triple - negative breast cancer was 19.9  months and 75% of recurrences occurred within 3  years of the diagnosis of breast cancer . our previous study concerning 222 patients with breast cancer and brain metastases revealed statistically significant differences in median os of patients with triple - negative , her2-positive and er / pr - positive her2-negative breast cancer . the differences were as follows : 2.8 , 4.3 and 5.4  years , respectively , . in the study by dent et al . , median os of triple - negative breast cancer was 4.2  years compared with 6  years for patients with other subtypes , and dfs was 2.6  years compared with 5  years in the other biological subtypes . in the present study , median survival from brain metastases in triple - negative breast cancer patients was 4  months . the results confirm our previous observations [ 2 , 3 ] and are comparable to the study done by lin et al . , in which median survival time from brain metastases was 4.9  months . the differences in survival between patients with basal - like and non - basal - like breast cancer have been assessed in some studies , but the results were not unequivocal . in some of them , basal - like breast cancer patients had a significantly shorter dfs than those lacking the expression of basal markers [ 24 , 25 ] . in the other studies , patients with basal - like breast cancer had significantly shorter dfs and os times than women with other biological types of breast cancer , but basal - like status was not a significant independent prognostic variable of os in the multivariate analysis [ 28 , 29 ] . in the study by rakha et al . , the difference in survival between basal - like and non - basal - like subtypes was assessed in two independent cohorts of triple - negative breast cancer patients . in the first cohort of 232 patients , after the time of observation of 207  months , the   basal - like subtype was associated with shorter dfs , but not os . in the second cohort of 67 triple - negative breast cancer patients , there was no significant difference in survival between those expressing basal markers and those not expressing them . in the present study , a very homogenous group of triple - negative breast cancer patients with brain metastases was assessed . this group had the worst prognosis out of all breast cancer patients because of the poorest biological subtype and the poorest site of dissemination . we did not show statistically significant differences in dfs , os and survival from brain metastases which could depend on the expression of basal markers . cox multivariate analysis revealed that the risk of death from brain metastases depends on well - known clinical features , but not on biological factors . the role of kps and the status of extracranial disease has been confirmed in our   and other studies . it is possible that , in the group with such poor outcome , molecular markers are less important than strong clinical prognostic factors . however , the small number of patients was a serious limitation of our study and that is why our results require confirmation by other authors . clinical characteristics of the entire group of 111 triple - negative breast cancer patients with brain metastases confirm the results of our previous studies [ 2 , 3 ] and are similar to the results of other authors [ 6 , 8 , 12 , 21 , 22 ] . due to the fact that we still do not know the biological or molecular factors that are responsible for dissemination to the brain and poor survival after the detection of brain metastases , we tried to find out if basal markers could play a major role in this processes . so we compared clinical features and survival of patients with triple - negative tumors and brain metastases which expressed and did not express basal markers . because it was not possible to perform dna microarray , we used ihc as a surrogate of molecular analysis . in our material , these results are in line with the findings of nielsen et al . , livacy et al .   and kreike et al . . her1 was expressed in 36% of tumors and it is also in agreement with the literature ; her1 was expressed in up to 66% of basal - like and triple - negative breast cancers , but her1 responsible for activating gene mutations were remarkably rare [ 16 , 21 , 2426 ] . the third basal marker , c - kit was expressed in 17% of tumors . it was less than in the study by nielsen et al .   and kreike . in our material , the proportion of patients with basal - like and non - basal - like phenotypes was 76% and 24% , respectively . this result was comparable to that observed by other authors [ 13 , 14 , 18 , 19 , 25 , 27 ] . however , in some papers , the percentage of patients with the basal - like subtype was about 50% . there are few data in the literature concerning differences between basal - like and non - basal - like subtypes of triple - negative breast cancer according to clinical features and survival . to our knowledge , the present study is the first one analyzing such relationships in patients with brain metastases . we did not observe statistically significant differences between basal - like and non - basal - like subtypes apart from the pattern of distant metastases . compared the clinicopathological and immunohistochemical features of triple - negative tumors depending on the expression of specific basal markers . the authors did not show any difference in morphological features between both subtypes , but basal - like breast cancer showed distinct immunophenotypical differences with the expression of several markers of poor prognosis . in addition , the authors observed a unique pattern of distant metastases of the basal - like subtype with more frequent metastasis to brain and lung . in the present study , in 35% of patients with the basal - like phenotype , the brain was the first site of distant metastasis . the high propensity of the basal - like phenotype to metastasize to the brain as the first site of distant recurrence is worth further molecular investigation . it is possible that some molecular markers allow anchor cancer cells into the brain ( theory of  soil and seed  ) . if we knew such a molecular marker , we would be able to select a group of breast cancer patients with high risk of brain metastases in order to take preventive or screening activities . in the present study , median dfs of all patients with triple - negative breast cancer with brain metastases was less than 2  years ( 20  months ) and os was less than 3  years ( 29  months ) . in which median dfs of patients with triple - negative breast cancer was 19.9  months and 75% of recurrences occurred within 3  years of the diagnosis of breast cancer . our previous study concerning 222 patients with breast cancer and brain metastases revealed statistically significant differences in median os of patients with triple - negative , her2-positive and er / pr - positive her2-negative breast cancer . the differences were as follows : 2.8 , 4.3 and 5.4  years , respectively , . in the study by dent et al . , median os of triple - negative breast cancer was 4.2  years compared with 6  years for patients with other subtypes , and dfs was 2.6  years compared with 5  years in the other biological subtypes . in the present study , median survival from brain metastases in triple - negative breast cancer patients was 4  months . the results confirm our previous observations [ 2 , 3 ] and are comparable to the study done by lin et al . , in which median survival time from brain metastases was 4.9  months . the differences in survival between patients with basal - like and non - basal - like breast cancer have been assessed in some studies , but the results were not unequivocal . in some of them , basal - like breast cancer patients had a significantly shorter dfs than those lacking the expression of basal markers [ 24 , 25 ] . in the other studies , patients with basal - like breast cancer had significantly shorter dfs and os times than women with other biological types of breast cancer , but basal - like status was not a significant independent prognostic variable of os in the multivariate analysis [ 28 , 29 ] . in the study by rakha et al . , the difference in survival between basal - like and non - basal - like subtypes was assessed in two independent cohorts of triple - negative breast cancer patients . in the first cohort of 232 patients , after the time of observation of 207  months , the   basal - like subtype was associated with shorter dfs , but not os . in the second cohort of 67 triple - negative breast cancer patients , there was no significant difference in survival between those expressing basal markers and those not expressing them . in the present study , a very homogenous group of triple - negative breast cancer patients with brain metastases was assessed . this group had the worst prognosis out of all breast cancer patients because of the poorest biological subtype and the poorest site of dissemination . we did not show statistically significant differences in dfs , os and survival from brain metastases which could depend on the expression of basal markers . cox multivariate analysis revealed that the risk of death from brain metastases depends on well - known clinical features , but not on biological factors . the role of kps and the status of extracranial disease has been confirmed in our   and other studies . it is possible that , in the group with such poor outcome , molecular markers are less important than strong clinical prognostic factors . however , the small number of patients was a serious limitation of our study and that is why our results require confirmation by other authors . triple - negative breast cancer with brain metastases is a highly selected type of cancer with very poor prognosis . the recurrence of the disease occurs within 2  years and overall survival is less than 3  years . basal - like and non - basal - like subgroups of patients with brain metastases did not differ significantly with regards to clinical features and survival except for the pattern of metastatic spread . factors influencing survival from brain metastases were kps and the status of the extracranial disease . searching for other biological markers responsible for metastatic spread to the brain is of special value .
the aim of this study was to divide the group of triple - negative breast cancer patients with brain metastases into basal - like and non - basal - like biological subtypes in order to compare clinical features and survival rates in those two groups . 
 a comprehensive analysis of 111 consecutive triple - negative breast cancer patients with brain metastases treated in the years 20032009 was performed . in 75 patients , 
 immunohistochemistry was used as a surrogate of microarray in order to evaluate the expression of three basal markers : cytokeratin 5/6 ( ck 5/6 ) , egfr / her1 and c - kit . 
 the basal - like ( er / pgr / her2-negative , ck5/6positive and/or her1-positive ) and non - basal - like ( er / pgr / her2-negative , ck5/6-negative , her1-negative ) subsets were selected . 
 clinical features and survivals were compared in both groups . in the group of 111 triple - negative breast cancer patients , median dfs , os and survival from brain metastases were 20 , 29 and 4  months , respectively . in 75 patients who were evaluable for basal markers , median dfs , os and survival from brain metastases were 18 , 26 and 3.2  months , respectively . in the basal - like subtype , 
 the survival rates were 15 , 26 and 3  months , respectively , and in the non - basal - like subtypes , they were 20 , 30 and 2.8  months , respectively . 
 no statistically significant differences in survivals were detected between the basal - like and non - basal - like biological subtypes . 
 factors influencing survival from brain metastases were : karnofsky performance status ( kps ) , the status of extracranial disease and age . 
 biological markers differentiating triple - negative group into basal - like and non - basal - like subtype ( ck 5/6 , her1 , c - kit ) had no influence on survival . in patients with triple - negative breast cancer and brain metastases , 
 well - known clinical , but not molecular , features correlated with survival .