strains of s. mutans were collected regularly from subjects of different ages from 4 years up to adulthood as part of one of a number of research projects aimed at assessing caries risk ( 15 , 16 , 18 , 20 ) . ethical permission for each study was obtained from the relevant authority in iceland as appropriate . following the completion of these individual studies a collection of 38 strains of s. mutans was established ( 24 from subjects with active caries and 14 from individuals that were caries - free according to the examination criteria used in each particular study ) . all strains were stored frozen at 80c in a preservative medium ( nunc cryotubes , fisher scientific loughborough , uk ) all personal identifiers were removed and the strain collection was merely grouped into the identification of the species as s. mutans or s. sobrinus and the disease category as either caries - free or considerable active caries above average for the age group . test strains were removed from the freezer , cultured for 48 h in todd - hewitt broth ( difco ) and subsequently incubated overnight in todd - hewitt broth containing tritiated thymidine and hydroxyapatite powder ( sigma - aldrich ) ( 24 ) . the cultures were shaken gently on a gyro rocker ( stuart scientific , keison international ltd , uk ) . following this treatment , the apatite powder was dissolved in 1n hcl and 0.25 ml aliquots transferred to a scintillation tube to which were added 1 ml hionic fluor scintillation fluid and the radioactive count recorded in a scintillation counter ( packard instruments , rockville , md , usa ) . the ability of strains of different s. mutans to decalcify hydroxyapatite was assessed using the method described by chestnutt et al . test strains from caries - active individuals ( 24 strains ) and caries - free individuals ( 14 strains ) aged 720 years were obtained from the strain collection and cultured for 24 h in todd - hewitt broth ( difco ) . cultures were centrifuged and 40 mg of bacterial deposit was resuspended in 400 l glucose to which 40 mg hydroxyapatite slurry ( sigma ) was added . this culture was incubated for 5 h at 37c on a gyro rocker ( stuart scientific , keison international ltd , uk ) . cultures were then centrifuged for 20 min at 15,000 rpm ( microspin 12s , sorvall instruments , uk ) . then the ph of the supernatant fluid was determined and the calcium concentration of the supernatant was determined using 10 l aliquots tested in a clinical chemistry autoanalyser ( kodak , rochester , usa ) at 680 nm . the bacteriocin - like inhibitory effect was tested , using the methods of rogers ( 22 ) and parrot et al . all eight test strains of s. mutans from caries - active individuals with open caries and the eight test strains from caries - free individuals were investigated against each other . thus each strain was tested for bacteriocin - like activity against 15 strains of s. mutans . in addition , 14 s. mutans strains ( seven from caries active and seven from caries - free individuals ) were tested using the same methodology against a laboratory collection of 25 oral microorganisms as listed in table 3 . all test strains were grown overnight in todd - hewitt broth , yielding approximately 10/cfu / ml , and 1 ml of each culture was used to inoculate pour - plates of semi - solid tryptic - soy - yeast - extract agar . in this role the strains were indicator strains of bacteriocin - like production . after the pour plates had set all of the test strains these pour plates were then incubated at 37c in a candle jar for 48 h and zones of inhibition of the indicator strain around the stab inoculations were viewed in a bifocal microscope and the diameter of the zone of inhibition measured . zones of inhibition of the indicator strains around the stab inoculated test strain ranged from 7 to 12 mm and all zones of diameter 7 mm or greater were recorded as positive for bacteriocin activity . test strains were removed from the freezer , cultured for 48 h in todd - hewitt broth ( difco ) and subsequently incubated overnight in todd - hewitt broth containing tritiated thymidine and hydroxyapatite powder ( sigma - aldrich ) ( 24 ) . the cultures were shaken gently on a gyro rocker ( stuart scientific , keison international ltd , uk ) . following this treatment , the apatite powder was dissolved in 1n hcl and 0.25 ml aliquots transferred to a scintillation tube to which were added 1 ml hionic fluor scintillation fluid and the radioactive count recorded in a scintillation counter ( packard instruments , rockville , md , usa ) . the ability of strains of different s. mutans to decalcify hydroxyapatite was assessed using the method described by chestnutt et al . test strains from caries - active individuals ( 24 strains ) and caries - free individuals ( 14 strains ) aged 720 years were obtained from the strain collection and cultured for 24 h in todd - hewitt broth ( difco ) . cultures were centrifuged and 40 mg of bacterial deposit was resuspended in 400 l glucose to which 40 mg hydroxyapatite slurry ( sigma ) was added . this culture was incubated for 5 h at 37c on a gyro rocker ( stuart scientific , keison international ltd , uk ) . cultures were then centrifuged for 20 min at 15,000 rpm ( microspin 12s , sorvall instruments , uk ) . then the ph of the supernatant fluid was determined and the calcium concentration of the supernatant was determined using 10 l aliquots tested in a clinical chemistry autoanalyser ( kodak , rochester , usa ) at 680 nm . the bacteriocin - like inhibitory effect was tested , using the methods of rogers ( 22 ) and parrot et al . all eight test strains of s. mutans from caries - active individuals with open caries and the eight test strains from caries - free individuals were investigated against each other . thus each strain was tested for bacteriocin - like activity against 15 strains of s. mutans . in addition , 14 s. mutans strains ( seven from caries active and seven from caries - free individuals ) were tested using the same methodology against a laboratory collection of 25 oral microorganisms as listed in table 3 . all test strains were grown overnight in todd - hewitt broth , yielding approximately 10/cfu / ml , and 1 ml of each culture was used to inoculate pour - plates of semi - solid tryptic - soy - yeast - extract agar . in this role after the pour plates had set all of the test strains were stab - inoculated into the agar surface of each pour plate . these pour plates were then incubated at 37c in a candle jar for 48 h and zones of inhibition of the indicator strain around the stab inoculations were viewed in a bifocal microscope and the diameter of the zone of inhibition measured . zones of inhibition of the indicator strains around the stab inoculated test strain ranged from 7 to 12 mm and all zones of diameter 7 mm or greater were recorded as positive for bacteriocin activity . strains of s. mutans isolated from caries - active individuals were found to adhere significantly better to apatite than did those strains isolated from caries - free subjects ( students t - test ; p<0.05 ) , see table 1 . scintillation counts for strains of s. mutans from caries - active and caries free subjects and adhering to hydroxyapatite strains of s. mutans from individuals with active caries were found to release significantly more calcium from hydroxyapatite than strains isolated from caries - free individuals ( students t - test ; p<0.02 ) , see table 2 . calcium released from hydroxyapatite by strains of s. mutans from caries - active and caries free subjects the ph of the individual cultures was measured at the end of the incubation period . considerable strain variation in the final ph was observed with ph values from caries - active individuals ranging from 4.1 to 4.9 ( mean 4.4 ; sd 0.23 ) and from caries - free individuals 4.25.1 ( mean 4.6 ; sd 0.19 ) but the difference in final ph between strains was significantly lower in the cultures containing strains from caries - active individuals ( students t - test ; p<0.01 ) . strains isolated from caries - active subjects showed greater bacteriocin - like activity against other isolates of s. mutans ( average 3.4 strains ) than did strains isolated from subjects that were caries free ( average 1.0 strains ; p<0.01(chi ) ; see fig . 1 ) . furthermore 7/8 strains from caries active individuals inhibited 2 other strains of s. mutans whereas only 1/8 strains from a caries - free individual inhibited 2 other strains of s. mutans . pattern of bacteriocin - like activity within strains of s. mutans ( strains 18 isolated from caries - free subjects and strains 916 from caries - active subjects ) . note that the strains from caries - active subjects produce bacteriocins that inhibit growth of more indicator strains than do strains isolated from caries - free subjects . when bacteriocin - like inhibition of 25 oral commensal organisms by s. mutans producer strains was tested ( seven strains from caries active individuals and seven strains from caries - free individuals ) the strains of s. mutans from caries - free individuals were found to be significantly more inhibitory to other oral commensals than the strains from caries active individuals ( p<0.001 ; see table 3 ) . bacteriocin - like activity against oral commensals by 14 strains of s. mutans , seven from caries - active subjects and seven from caries - free subjects thus the seven s. mutans strains from caries - free individuals inhibited other oral commensals in a total of 88/126 tests whereas the seven s. mutans strains inhibited from caries - active individuals inhibited oral commensals in only 58/126 tests ( t - test ; p<0.05 ) strains of s. mutans isolated from caries - active individuals were found to adhere significantly better to apatite than did those strains isolated from caries - free subjects ( students t - test ; p<0.05 ) , see table 1 . scintillation counts for strains of s. mutans from caries - active and caries free subjects and adhering to hydroxyapatite strains of s. mutans from individuals with active caries were found to release significantly more calcium from hydroxyapatite than strains isolated from caries - free individuals ( students t - test ; p<0.02 ) , see table 2 . calcium released from hydroxyapatite by strains of s. mutans from caries - active and caries free subjects the ph of the individual cultures was measured at the end of the incubation period . considerable strain variation in the final ph was observed with ph values from caries - active individuals ranging from 4.1 to 4.9 ( mean 4.4 ; sd 0.23 ) and from caries - free individuals 4.25.1 ( mean 4.6 ; sd 0.19 ) but the difference in final ph between strains was significantly lower in the cultures containing strains from caries - active individuals ( students t - test ; p<0.01 ) . strains isolated from caries - active subjects showed greater bacteriocin - like activity against other isolates of s. mutans ( average 3.4 strains ) than did strains isolated from subjects that were caries free ( average 1.0 strains ; p<0.01(chi ) ; see fig . 1 ) . furthermore 7/8 strains from caries active individuals inhibited 2 other strains of s. mutans whereas only 1/8 strains from a caries - free individual inhibited 2 other strains of s. mutans . pattern of bacteriocin - like activity within strains of s. mutans ( strains 18 isolated from caries - free subjects and strains 916 from caries - active subjects ) . note that the strains from caries - active subjects produce bacteriocins that inhibit growth of more indicator strains than do strains isolated from caries - free subjects . when bacteriocin - like inhibition of 25 oral commensal organisms by s. mutans producer strains was tested ( seven strains from caries active individuals and seven strains from caries - free individuals ) the strains of s. mutans from caries - free individuals were found to be significantly more inhibitory to other oral commensals than the strains from caries active individuals ( p<0.001 ; see table 3 ) . bacteriocin - like activity against oral commensals by 14 strains of s. mutans , seven from caries - active subjects and seven from caries - free subjects thus the seven s. mutans strains from caries - free individuals inhibited other oral commensals in a total of 88/126 tests whereas the seven s. mutans strains inhibited from caries - active individuals inhibited oral commensals in only 58/126 tests ( t - test ; p<0.05 ) intitially studies of the role of s. mutans in caries in iceland was expected to confirm the pathogenic role of this organism and indeed it was hoped to add to our knowledge of the pathogenic capacity of this bacterium in the light of the high levels of caries that were being investigated . the findings of khler and krasse ( 21 ) that an icelandic strain of s. mutans was particularly cariogenic in an animal model reinforced this line of thought . laboratory studies then demonstrated that icelandic strains of s. mutans from subjects with active caries indeed adhered significantly better to hydroxyapatite and decalcified hydroxyapatite significantly more than strains isolated from subjects that were caries free . furthermore the prevalence of s. sobrinus had been found in several studies ( 1820 ) to be relatively high in the icelandic population , reinforcing the view that the teeth of icelanders were in a rather more cariogenic environment than was found in many other studies being carried out at this time . with the development of the ecological plaque hypothesis ( 27 , 28 ) the emphasis on the differing cariogenic potentials of strains of mutans streptococci began to wane . studies of bacteriocin - like activity among the icelandic test strains suggested indeed that the strains of s. mutans from caries active subjects were more aggressive at inhibiting other strains of s. mutans than those strains isolated from caries - free individuals . thus s. mutans from caries - active individuals may dominate the less cariogenic strains of the same species and succeed in finding space to adhere and multiply within the cariogenic dental plaque biofilm . in caries - free individuals , however , the apparently less cariogenic strains of s. mutans appear to compete more successfully against other oral commensals than do the more cariogenic strains of s. mutans . these less cariogenic strains then occupy the niche for s. mutans - like organism within the dental plaque biofilm and thus help maintain its low cariogenicity . several studies of bacteriocin - like properties have suggested a significant role for this activity in the ecology of dental plaque ( 7 , 2931 ) . it is quite possible that the differing degrees of cariogenicity seen in s. mutans strains from caries - active and caries - free subjects could be a reflection of the oral/ dental environment from which the organisms were originally isolated rather than a genetically determined pathogenic potential . thus s. mutans bacteria in a dental biofilm that has ready access to sucrose could have several metabolic pathways working that contribute to the cariogenicity of the organism whereas s. mutans strains from a biofilm that is less exposed to sucrose , for example , may have to compete with other organisms to retain a place in the biofilm but this possibly results in less expression of the cariogenic potential of the s. mutans strain . in developing this hypothesis further the authors were fortunate in being asked to submit strains of s. mutans from this collection to an international study of possible genetic differences between strains isolated from caries - active and caries - free individuals . ( 32 ) and showed no genetic differences between strains and thus reinforces somewhat the ecological plaque hypothesis and the idea that the increased cariogenic properties found in strains of s. mutans from caries - active subjects are manifestations of phenotypic characteristics related to the environment from which the strains were sampled . it may also serve to illustrate the considerable potential for adaptability of strains of s. mutans in the oral cavity . there is no conflict of interest in the present study for either of the authors .
backgrounddental caries has been strongly associated with mutans streptococci , particularly streptococcus mutans and s. sobrinus . 
 many studies have linked these organisms to the carious process and counts of mutans streptococci have been used to monitor caries risk . 
 the high levels of caries generally found in iceland have enabled several studies to be performed on the variation within strains of s. mutans.methodsthis paper reports some studies showing phenotypic differences between strains of s. mutans that were related to whether the strain was isolated from an individual with active caries or from a caries - free subject.resultsstrains from individuals with active caries generally adhered better to apatite , were more vigorous in decalcifying apatite and had bacteriocin - like activity that was likely to help the strain compete successfully with other strains , for example in the dental plaque biofilm.conclusionsphenotypic differences exist between strains of s. mutans depending on the caries activity of the individual from whom the strain was isolated .