to compare direct and indirect bead - based t - cell isolation followed by magnetic bead - based dna isolation . t - cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead - based automated dna isolation in 10 healthy subjects . purity of t - cells , purity of dna ( by a260/a280 ratio measurement ) and dna concentration were assessed . direct and indirect labelling resulted in comparable t - cell purity ( 93.111.47% vs. 94.991.54% , p= 0.125 ) and dna concentration per cell ( 50.9714.15 ng/(mlxcell ) vs. 49.5313.62 ng/(mlxcell ) , p=0.492 ) , while dna purity was significantly higher after direct labelling ( 1.820.05 vs. 1.780.03 , p=0.0488 ) . both direct and indirect magnetic bead - based t - cell selection may be used prior to magnetic bead - based dna isolation procedures .
objectiveto compare direct and indirect bead - based t - cell isolation followed by magnetic bead - based dna isolation.methodst-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead - based automated dna isolation in 10 healthy subjects . 
 purity of t - cells , purity of dna ( by a260/a280 ratio measurement ) and dna concentration were assessed.resultsdirect and indirect labelling resulted in comparable t - cell purity ( 93.111.47% vs. 94.991.54% , p= 0.125 ) and dna concentration per cell ( 50.9714.15 ng/(mlxcell ) vs. 49.5313.62 ng/(mlxcell ) , p=0.492 ) , while dna purity was significantly higher after direct labelling ( 1.820.05 vs. 1.780.03 , p=0.0488).conclusionsboth direct and indirect magnetic bead - based t - cell selection may be used prior to magnetic bead - based dna isolation procedures .