moderate traumatic physical challenge has been frequently used as a therapeutic modality in traditional chinese medicine , which leads to an anticipation of beneficial health outcome following muscle - damaging exercise . we have previously reported an increased necrosis and white blood cell infiltration in exercised muscle after downhill running , an exercise regimen containing eccentric muscle contraction . however , decreased insulin sensitivity in glucose transport and impaired glycogen storage of skeletal muscle after eccentric muscle contraction has been reported in animals and humans.4 , 5 skeletal muscle , accounted for 40% of body weight , is the major tissue for post - meal glucose uptake . the whole - body insulin sensitivity , assessed by hyperinsulinemic euglycemic clamp technique , suggests that insulin resistance occurs during the first 48  h after eccentric exercise.6 , 7 decreased muscle insulin sensitivity is expected to produce a negative consequence in the whole - body glycemic regulation . based on these findings , we hypothesized that muscle - damaging exercise training will decrease glucose tolerance in men . blood creatine kinase ( ck ) level has been commonly used to measure the levels of exercise - induced muscle damage . histological analysis suggests that blood level of muscle ck should be regarded as a biomarker for post - exercise muscle regeneration.2 , 8 in this study , we assessed oral glucose tolerance together with muscle ck in plasma , two days following the last bout of exercise training containing eccentric muscle contraction . we tested the hypothesis that 1 ) muscle - damaging exercise will decrease the whole - body glucose tolerance ; 2 ) the degree of ck increase is associated with attenuated glucose tolerance in men . twenty - one healthy junior athletes ( 16.3    0.5 years of age ) with no history of musculoskeletal disorders of the lower limbs were enrolled in this study . participants performed barbell front squat and downhill run for 3 consecutive days then allowed to rest for another 2 days ( fig .  1 ) . for ogtt , blood was collected at day 1 before exercise challenge and again at day 4 , 12  h after the last bout of training . to verify the result of muscle damage , blood sample was collected at day 1 ( 24  h ) and day 2 ( 48  h ) for ck analysis after the last exercise bout . participants were instructed to perform 5 sets of barbell front squat for 20 repetitions at 30 pounds a day for 3 consecutive days and allowed to rest for another 3 consecutive days . the 100-meter downhill sprinting consisted of 5 repetitions a day for 3 consecutive days and allowed to rest for another 3 consecutive days . under a 12-h overnight fasted condition , a 500-ml solution containing 75  g of glucose was orally delivered , and blood was taken from the finger at 0 ( before the solution load ) , 30 , 60 and 90  min afterward . blood glucose level was determined by an automated glucose analyzer ( lifescan , inc . , ck ( ckmb isoform ) in plasma is an indicator of muscle regeneration after exercise - induced muscle damage . plasma was obtained by centrifuging the blood at 4  c for 10  min  at 3000  rpm and was stored at  80  c until analysis . ck activity was measured using a dt-60 automated hematology analyzer ( kodak co. , rochester , ny , usa ) . student 's t - test was used to compare the mean difference in each variable between the two groups ( low ck group vs. high ck group ) . statistical significance for type i error was set at p  <  0.05 for all measures . the current exercise challenge protocol ( fig .  1 ) results in a wide range of muscle ck surge ( table  1 ) . for those participants showing relatively lower ck increases ( fig . 2a ) , glucose levels during ogtt decreased significantly after exercise , indicating an improvement in the glucose tolerance . 2b ) , similar magnitude of improvement on glucose level during ogtt was also reached . data on area under curve ( auc ) in ogtt show no difference in improvement of glycemic control between two groups ( fig . it has been shown that aerobic endurance exercise , as an exercise regimen producing insignificant muscle damage , can improve glucose tolerance . on the other hand , muscle - damaging eccentric muscle contraction results in insulin resistance in glucose transport and glycogen storage of exercised skeletal muscle.4 , 5 based on these early findings , we hypothesized that muscle - damaging exercise will decrease glucose tolerance in humans . our data suggest the benefit of improving glycemic control by a muscle - damaging exercise . therefore , insulin sensitivity of skeletal muscle should significantly influence the whole - body glucose tolerance . the previous report on onset of muscle insulin resistance after eccentric muscle contraction appears to be associated with decreases in glucose transporter glut4 protein in skeletal muscle.4 , 5 , 6 one possibility to explain this discrepancy may be associated with the time after the first bout of exercise challenge . in the current study , our participants conducted 3-day downhill running and measurements were conducted 2 days after the last bout of exercise . glycemic condition may be changing overtime during different stage of the post - exercise recovery . in addition , it has been reported that a single bout of eccentric exercise can result in an increased insulin secretion in response to hyperglycemia . this is based on results from a hyperglycemic clamp assessment , which shows an initial elevation in insulin release at the first 10  min of glucose infusion . our current data show decreased blood glucose during ogtt , suggesting that either insulin sensitivity or increased insulin secretion must take place to lower blood glucose levels . therefore , the result of lower glucose response in ogtt after the muscle damaging exercise is likely associated with concurrent increased response of insulin secretion from pancreatic beta cells and improved insulin sensitivity of exercised muscle . in this study a wide spectrum of muscle damage was produced among different participants . based on our hypothesis , if muscle damage can lower insulin sensitivity , we should observe differences in ogtt response between high ck and low ck group . the most likely explanation is that the exercise challenge damaged relatively weaker muscle fiber with insufficient metabolic function . it is quite easy to perceive that healthy muscle fibers have greater chance of survival after an entropic challenge . there may be a great variation in the proportion of unhealthy versus healthy muscle fibers among participants due to individual 's variation in training status or lifestyle . thus the exercise challenge lowers the unhealthy population of muscle fibers of all participants to a similar level , regardless their pre - existing percentage of metabolically weaker muscle fibers . human body may be considered as a society composed by cells , where the principle of darwinian natural selection should apply . it has been shown that all - cause mortality decreases as exercise intensity increases,11 , 12 suggesting that removing relatively less robust cells in human body by greater challenge can bring more beneficial outcome . we speculate that enhance muscle fiber renewal may be the most important mechanism accounted for the beneficial outcome of the entropic challenge in glycemic control . skeletal muscle cells in human body are relatively short - lived compared to brain and is continuously dying and regenerating overtime . during early stage of muscle development , glut1 protein ( low km transporter ) is predominantly expressed followed by increasing glut4 expression as muscle mature . therefore , the knowledge generalized from the study for older population deserves more experimental confirmation . it is generally known that the individuals having greater needs for better glycemic control are those middle - aged or elderly people . therefore , the current result does not provide direct answer with regard to whether muscle - damaging exercise is able to improve glucose tolerance at higher age level . in conclusion , according to previous finding on decreased insulin sensitivity of skeletal muscle after eccentric muscle damaging exercise , we hypothesized that downhill running with resistance exercise will result in deterioration in ogtt . this finding implicates that metabolically weaker muscle fibers are replaced by newly generated cell population after muscle - damaging eccentric exercise .
tissue damage is regarded as an unwanted medical condition to be avoided . however , introducing tolerable tissue damages has been used as a therapeutic intervention in traditional and complementary medicine to cure discomfort and illness . 
 eccentric exercise is known to cause significant necrosis and insulin resistance of skeletal muscle . 
 the purpose of this study was to determine the magnitude of muscle damage and blood glucose responses during an oral glucose tolerance test ( ogtt ) after eccentric training in 21 young participants . 
 they were challenged by 5 times of 100-meter downhill sprinting and 20 times of squats training at 30 pounds weight load for 3 days , which resulted in a wide spectrum of muscle creatine kinase ( ck ) surges in plasma , 48  h after the last bout of exercise . 
 participants were then divided into two groups according the magnitude of ck increases ( low ck :  + 48%    0.3 ; high ck :  + 137%    0.5 , p  <  0.05 ) . 
 both groups show comparable decreases in blood glucose levels in ogtt , suggesting that this muscle - damaging exercise does not appear to decrease but rather improve glycemic control in men . 
 conclusion : the result of the study rejects the hypothesis that eccentric exercise decreases glucose tolerance . 
 improved glucose tolerance with ck increase implicates a beneficial effect of replacing metabolically weaker muscle fibers by eccentric exercise in darwinian natural selection fashion .