severe sepsis is a systemic inflammatory response secondary to infection and associated with the acute dysfunction of one or more organs.1 the disease is highly aggressive and may involve the progressive loss of function of several organs , but it should be considered reversible , especially if identified and treated early in its course . it is estimated that 750,000 new cases of sepsis occur yearly in the usa , with roughly 215,000 deaths per year . in the last decade , its incidence has increased by about 91.3%.2 according to the bases study,3 27% of the patients referred to intensive care units in brazil have severe sepsis , with a mortality rate of 47% at 28 days . furthermore , sepsis substantially reduces the quality of life of those who survive.4 several factors explain the increased incidence of sepsis , including the aging population , the increasing survival of patients who have cancer or are immunosuppressed , and the increasing numbers of invasive medical interventions.5 the septic patient is a constant challenge to the clinician and an early diagnosis is fundamental6 to its successful treatment . even though a large volume of information has been collected about this topic,711 the lack of knowledge about the diagnostic criteria for sepsis by emergency teams is one of the greatest factors limiting its adequate treatment.12 when evaluating the efficacy of a specific treatment protocol for severe sepsis / septic shock in the emergency department ( ed ) , rivers et al . demonstrated a significant reduction in hospital mortality rate.13 though septic patients admitted to the hospital are first examined in the ed , there are few hospitals that adopt a diagnostic approach with aggressive therapy in the ed . considering the lack of epidemiological studies involving severe sepsis / septic shock patients in the ed , the aim of the study was to determine the occurrence rate , demographic data , clinical characteristics , and outcomes of patients with severe sepsis admitted to the emergency department ( ed ) of a tertiary hospital . after approval by the institution s internal review board , which did not require obtaining patients informed consent , the study was conducted in the hospital s emergency department , a 50-bed unit for severely ill patients . the medical team of the ed is composed of medical residents directed by clinicians and surgeons of several medical specialties . patients with severe sepsis / septic shock were included based on the sccm / accp consensus 1991 criteria ( table 1).14 in an attempt to avoid seasonal differences , patients admitted to the ed during two distinct periods  february 1 to april 30 , and july 1 to september 30 , 2004  were included . the exclusion criteria were : patients younger than 18 years old and patients with terminal disease ( moribund ) who did not survive at least 24 hours after admission to the ed because of their underlying diseases ( where management of their sepsis would not likely have helped ) . fellows specially trained to diagnose severe sepsis went to the ed four times a day to search for patients who fulfilled severe sepsis criteria . these patients were included in the study , and an arterial blood sample was collected in order to evaluate the arterial lactate level as a marker of organ dysfunction resulting from metabolic acidosis . instances where the ed team evaluated a chart referring to septicemia , generalized infection , sepsis , severe sepsis , or septic shock to make a diagnosis were considered diagnoses of sepsis . in addition , whether the ed team had prescribed any antibiotics as well as the amount of fluid administered in the first six hours was noted . if all care with regard to treatment of sepsis was performed by the ed team according to the surviving sepsis campaign guidelines15,16 , this was also considered to be a sepsis diagnosis . in order to standardize data collection , the worst physiologic and laboratory values from the last 24 hours before study inclusion while patients were still in the ed were used to calculate the sofa17 and apache ii18 scores . in patients who were sedated , the glasgow coma score was measured prior to sedation and registered in the chart . moreover , due to the unsatisfactory initial patient mortality results of the study , the institutional ethics committee and researchers decided to offer training to the ed team . the training provided instructions about sepsis diagnosis , according to the ssccm / accp consensus 1991 criteria9 and it also provided instruction about sepsis treatment as recommended by the surviving sepsis campaign guidelines.1011 the training included lectures , folders , and pocket references . after training the ed team , we checked whether sepsis diagnosis and treatment , according to the surviving sepsis campaign guidelines1516 , had improved compared to diagnosis and treatment before training . demographic data were expressed as means  standard deviation , medians ( interquartile interval ) , or percentages ; 95% confidence intervals were estimated to correctly classify outcomes . means of quantitative variables were compared between two independent groups using the student s t - test . variables with nonsymmetrical distributions were evaluated using the mann - whitney test and the friedman test . analysis to identify independent risk factors and to control for confounding effects ( mutually adjusted variables ) . variables considered statistically significant ( p < 0.05 ) in the univariate analysis were considered as candidates using the multiple regression model . every statistical test was two - tailed , and a significance level of 0.05 was used . during the study period , 5332 patients were admitted to the ed and 369 ( 6.9% ) of them fulfilled the criteria for severe sepsis , but of those , only 342 ( 6.4% ) patients met inclusion criteria and 27 ( 0.5% ) patients were excluded because they presented with serious underlying diseases . the occurrence rate of severe sepsis in the ed during the study is presented in figure 1 . patients with severe sepsis admitted to the ed had a mean age of 73.713.5 years , and 52.6% were male . the median apache ii and sofa scores were 19 ( 1525 ) and 5 ( 37 ) , respectively . patients had a median of 2 ( 13 ) dysfunctional organs ( table 1 ) . the median length of hospital stay was 10 ( 4.717 ) days , and the hospital mortality rate was 64% . the main infection sites were : lower respiratory tract , n=194 ( 56.7% ) , followed by urinary tract , n=72 ( 21.1% ) and intra - abdominal infections , n=37 ( 10.8% ) . twenty - seven ( 7.9% ) patients presented with catheter - related bloodstream infections , catheter insertion site infections , or infection in other sites , such as the central nervous system , skin , blood , bones , joints or the heart . the main physiologic and laboratory variables at the inclusion in the study are presented in table 2 . at the time of inclusion , 39.8% of patients showed one dysfunctional organ or system , whereas 27.8% of patients presented two dysfunctions ; three dysfunctional organs or systems were seen in 20.7% ; four or five dysfunctions were present in 9.4% of patients ; and six were present in 2.3% of patients . about 90% of the patients received antibiotics prescribed by the ed physicians , but the amount of administered fluids ( crystalloids ) during the first six hours was only 500 ( 5001006.25 ) ml . no colloids were prescribed during the first six hours of treatment . among the 342 patients with severe sepsis in the ed , only 115 ( 33.5% ) were referred to the icu . the variables considered statistically different between survivors and nonsurvivors were : age , male gender , apache ii and sofa scores , pulmonary and urinary infectious focus , arterial lactate level , number of organ dysfunctions ( metabolic , cardiovascular , neurological , renal , hepatic and respiratory dysfunction ) , as well as diagnosis by the ed team with referral to the icu , and mean , systolic , and diastolic arterial pressures ( table 3 ) . in the logistic regression , only the discriminative variables from the univariate analysis were considered . age , apache ii , male gender , systolic pressure and number of organ dysfunctions were independent variables for the risk of death in the ed ( table 4 ) . the diagnosis of severe sepsis defined by the ed team was made mainly in patients that had a large number of organ dysfunctions  generally , four , five or six ( figure 3 ) . in the first three months of the study , only 15.8% of patients with severe sepsis were diagnosed correctly by the medical team from the ed . considering this low rate , which demonstrated the ed team s difficulty in correctly diagnosing sepsis , we started training the ed team in order to improve their skills in this area . after this training , 38.5% of severe septic patients were diagnosed in the ed ( p<0.001 ; figure 2 ) . the median time between admission to the ed and arrival in the icu was 24 ( 1248 ) hours ; hence , the time to arrive in the icu decreased after training , with a median delay time of 48 hours before training and 12 hours after training ( figure 4 ) . in addition , the mortality rate decreased significantly , from 71.4% before training to 50.9% after training ( p=0.03 ) . our study is the first prospective cohort study in brazil performed exclusively in an emergency department to evaluate the characteristics and prognostic factors of this population . of the 5532 patients admitted to the ed only 34% of those patients were admitted to the icu , indicating a low rate of sepsis treatment in the icu . sepsis is a major reason for admission to the icu and is the leading cause of death in noncoronary icu patients and a common cause of death among hospitalized patients.19 many sepsis cases are belatedly recognized , and patients are treated inadequately by physicians outside the icu who are not familiar with the symptoms and signs of the disease . sepsis acquires a greater epidemiological importance every year , and the vast majority of studies report rates of 10  4 cases per 100 admissions to the icu.20 several epidemiological reports on sepsis have appeared in recent years , after the publication of the definitions of the accp / sccm consensus in 1991.9 however , those studies were exclusively done in the icu setting . our study is different , as it allowed us to analyze patients with severe sepsis prior to icu admission and allowed for inclusion of all non - terminal patients who died in the ed . this is not observed in epidemiological studies that have evaluated the incidence of sepsis in icu patients only . in comparison to our study , a french study21 with 11828 selected patients found 742 severe sepsis cases and an incidence rate of 14.6% . a study in oceania with 5878 patients reported 691 cases of severe sepsis and an incidence rate of 11.8%.22 in addition , there is a united kingdom ( uk ) study involving 56673 selected patients , with an incidence rate of 27.1%.23 in the usa , the rate of sepsis treatment was 82.7 cases per 100,000 in inhabitants in the period between 1979 and 1984 , rising to 240.4 between 1995 and 2000.23 in brazil , epidemiological data are scarce . according to the bases study3 of the southern and southeastern regions of the country , 241 of 1383 patients admitted to icus ( 27.3% ) the severe sepsis mortality rate has ranged from 18 and 52% of the cases in different studies.15 this rate has probably been underestimated because the majority of the patients are not admitted to the icu and are not even diagnosed by the ed team . in this study , we observed that the in - hospital mortality rate was 64% , greater than that detected in previous reports , with a median hospital length of stay very close to the global median of 10 days . in other words , even when the incidence of severe sepsis is not neglected , the mortality rate can be very high . in brazil3 and the uk23 , the admission rates of severely septic patients to icus are proportionally high . these countries have a smaller number of available icu beds than the usa.24 consequently , the admission to the icu of patients that require more attention , such as septic shocked patients with multiple organ dysfunctions , diminishes the number of beds available for patients who have less severe sepsis . by comparison , in the usa , patients are admitted early to icus , with fewer requirements of critical care facilities , such as mechanical ventilation . they have low mortality rates ( 17.9 to 27.8%).25,26 thus , in brazil and the uk , icu beds are spared for sicker patients , with greater resulting mortality rates ( 47% ) . the ed , therefore , can be an important setting in which to treat sepsis . this is particularly important in countries with scarce resources and few available icu beds as there , patients must be treated earlier , often while still in the ed . in the present study , patients with severe sepsis had a mean age of 74 years . in contrast , the majority of studies have only considered patients treated in the icu , who were around 65 years old . angus et al.27 suggested that age is an important independent factor of mortality in patients with sepsis . in the present study , age as a variable the fact that patients in the present study were older than in other studies suggests the possibility that a natural process is occurring : older patients with sepsis admitted to the ed have fewer functional reserves and progress quickly to poor outcomes , without admission to the icu . we also found at the ed that systolic pressure was an important factor in mortality and that patients with lower systolic pressures had a worse prognosis , even though we need to keep in mind that the clinical importance of this factor is low in our study because each increase of 1 mmhg in systolic pressure decreased the risk of death by only 0.02% . interestingly , systolic pressure was better than the mean pressure in predicting mortality . le gall et al . developed a severity score known as lods ( logistic organ dysfunction system),28 in which they used logistic regression to demonstrate that systolic pressure has great importance in evaluating cardiovascular function . moreover , the systolic pressure is the most important determinant of the left cardiac systolic volume and has also been considered as a variable of preload for some researchers.29,30 thus , close monitoring of systolic pressure could be useful in patients with severe sepsis . in addition , several studies have described gender differences in adult mortality related to cardiovascular events , with several studies suggesting that females may have lower mortality.31 in this study , a higher mortality was seen in males than females . this result suggests that sex steroids could modulate the inflammatory response and outcome after injury . furthermore , patients with one or two ( 2.11.1 ) dysfunctional organs and a median sofa score of 5 ( 37 ) were the most prevalent in our study . in the present study , the prognosis worsened as the number of dysfunctional organs increased , as demonstrated by the multivariate analysis ( odds ratio = 1.656 ) . a french study involving 35 icus revealed that patients with severe sepsis and two or more dysfunctional organs had a greater mortality rate.32 in our study , the ability of the ed team to diagnose severe sepsis was poor ( only 31% of the septic patients as possible is fundamental to the quick initiation of treatment , as demonstrated by rivers et al.8 in the present study , the diagnosis of severe sepsis was made more frequently in the most severely ill patients , who had a greater number of organ dysfunctions . in an international study with 1058 physicians , observed that the definitions of sepsis in the 1991 accp / sccm consensus are not well known compared to those of other specialties , resulting in a poor ability to diagnose sepsis.33 the symptoms and signs are easily confounded by other diseases , making diagnosis very difficult . sepsis tends to be detected only when multiple organ dysfunctions are seen , compromising the proper treatment . thus , early markers of the disease are necessary , and several studies have noted the usefulness of tissue perfusion markers such as serum arterial lactate,34,35 central or mixed venous saturation,8 base excess,36 pco2 gap,37 or metabolic variables , such as glycemia as potential early markers.38 vital signs such as arterial pressure or diuresis are later markers and are often unchanged , even in patients in septic shock.39 the incidence of severe sepsis in the studied population was 6.4% , but the ed team diagnosed it in less than one - third of the affected patients . thus , the severe sepsis diagnosis level in the ed was very low ( 15.8% ) . the in - hospital mortality rate in this sample was large , and only one - third of the patients were referred to the icu . furthermore , possible seasonal interferences were avoided in this study , because they already had been reported in a previous research.40 the epidemiological studies that have considered the incidence of severe sepsis treated in icus may have evaluated only a small part of a major problem . this could be explained based on findings observed in our study , that many patients with severe sepsis frequently are admitted to the ed without being referred to the icu . however , tertiary teaching hospitals frequently receive the majority of the most severe sepsis cases , including patients with multiple organ dysfunctions secondary to sepsis . another limitation is that the collection of comorbidities was not foreseen , and therefore not planned for in our protocol . age , apache ii score , male gender , number of organ dysfunctions , and systolic pressure were independent factors associated with a greater risk of mortality in patients with severe sepsis in our study . . these campaigns must include ed training , so that teams responsible for the initial evaluation can adequately recognize and treat patients during the so called golden hour .
backgroundthe aim of this study was to determine the occurrence rate , demographics , clinical characteristics , and outcomes of patients with severe sepsis admitted to the emergency department.methodsa prospective study evaluating all patients admitted to the emergency department unit in a public hospital of tertiary complexity in a six - month period was conducted . during this period , 
 the emergency team was trained to diagnose sepsis . 
 patients who met the diagnostic criteria for severe sepsis were followed until their discharge from the hospital.resultsa total of 5,332 patients were admitted to the emergency department , and 342 met the criteria for severe sepsis / septic shock . 
 the median ( interquartile range ) age of patients was 74 ( 6584 ) years , and 52.1% were male . 
 the median apache ii and sofa scores at diagnosis were 19 ( 1525 ) and 5 ( 37 ) , respectively . 
 the median number of dysfunctional organ systems per patient was 2 ( 13 ) . 
 the median hospital length of stay was 10 ( 4.717 ) days , and the hospital mortality rate was 64% . 
 only 31% of the patients were diagnosed by the emergency department team as septic . about 33.5% of the 342 severe sepsis patients admitted to the emergency department 
 were referred to an icu , with a median time delay of 24 ( 1248 ) hours . 
 training improved diagnosis and decreased the time delay for septic patients in arriving at the icu.conclusionsthe occurrence rate of severe sepsis in the emergency department was 6.4% , and the rate of sepsis diagnosed by the emergency department team as well as the number of patients transferred to the icu was very low . 
 educational campaigns are important to improve diagnosis and , hence , treatment of severe sepsis .