more than 2.1 billion people  close to 30% of the global population  today are overweight or obese.1 obesity can therefore be seen as a global epidemic and thus as a major public health problem . both overweight and obesity are characterized by the accumulation of excessive levels of body fat , and this creates an increased risk for cardiovascular diseases , some types of cancer , and overall mortality.2 recent evidence has shown similar adverse effects of obesity on the brain.3 in particular , abdominal obesity is associated with significant metabolic changes that impinge upon the central nervous system in even younger , nonclinical (= general ) populations at midlife.4 furthermore , obesity has been implicated to play a role in cognitive deficits in patients with dementia5 and mental disorders.6 the underlying reasons for becoming obese are not well understood ; however , it is likely that both genes and environmental factors , especially an age - related decrease in physical activity , contribute to this problem.7 obesity is caused by perturbations of the balance between food intake and energy expenditure . enlarged adipocytes and activated macrophages secrete proteins and lipids , known as adipokines , that influence inflammation and overall carbohydrate and lipid metabolism.8 moreover , an upregulation of indoleamine 2,3-dioxygenase-1 ( ido1 ) activity , caused by immune - mediated inflammation , which catalyzes the formation of kynurenine ( kyn ) and limits the availability of tryptophan ( trp ) , could be a key component in the initiation of obesity.9 thereby , the kyn pathway is induced,10 and the elevation of the ratio of kyn to trp concentrations that estimates the trp breakdown rate is often linked with conditions of inflammation11 and neuropsychiatric symptoms.12 the accelerated trp breakdown during inflammation is mainly fed by endogenously formed interferon- and is also influenced by other cytokines , such as tumor necrosis factor- , interleukin ( il)-6 , and lipopolysaccharide.13,14 as a precursor for 5-hydroxytryptamine ( serotonin ) , the essential amino acid trp is also a key player in caloric intake regulation by predominantly inhibiting carbohydrate intake.15 in the epiphysis , the sleep - regulating hormone melatonin is biosynthesized out from serotonin . lifestyle modification , specifically changes in diet , physical activity , and exercise are considered the cornerstone of obesity management.16 however , the adherence of victims to diet and exercise regimens is often limited and they may quit after the first six months before reaching their goals.17 recently , we observed that low - calorie weight loss diet lowered not only levels of leptin but also levels of trp significantly.18 the disturbed metabolism of trp might affect the biosynthesis of serotonin and could thereby increase the susceptibility for mood disturbances and carbohydrate craving , increasing the cessation probability of weight reduction programs ( fig . 1 ) . on the other side , moderate physical exercise  a potent stimulus to modulate proinflammatory cytokines , which may affect trp levels  could be helpful in improving mood status and preventing uncontrolled weight gain.19 the purpose of this review is to provide a brief overview on the effects of diet and exercise on weight control and to describe the important role of trp metabolism during dietetic interventions combined or not with physical exercise . a review of literature was conducted through pubmed database to identify relevant studies , especially published in the past 10 years . the following keywords were used alone or in various combinations : diet , exercise , mood , tryptophan , weight loss , and maintenance . reference lists from original and review articles were also reviewed in order to identify additional relevant studies . mechanistic influences on energy imbalance arise from changes in resting metabolic rate , nonexercise activity thermogenesis , fat - free mass , and energy intake . the impact of these changes on energy imbalance translates to changes in body weight.20 dietary restriction and exercise are useful methods to create a negative energy balance . however , most of the exercise studies suggest that exercise activity alone has only a minor influence on body weight reduction21 and that mainly the combination of both diet and exercise training leads to significant reduction of body mass.22 the classic explanation for the secondary role of exercise is that exercise alone can not generate enough energy expenditure to create a negative energy balance to the extent possible with caloric restriction . the frequency and intensity of exercise at public health recommendation levels may explain why exercise does not improve weight loss above that achieved with caloric restriction . ross et al.23,24 showed that the energy expenditure of exercise has to be between 500 and 700 kcal / d to provide a body mass loss of approximately 6 kg in women and 8 kg in men within 12 weeks . this is true , but it is also true that , independent of the method of weight loss , a negative energy balance of 500700 kcal / d always causes a fat loss of 68 kg within three months . when the energy deficit imposed by diet only and diet plus exercise interventions is similar , weight loss and/or percentage change in body weight are similar.25 nevertheless , to be successful in losing weight , it takes a considerable amount of time and effort . several studies have examined weight compensation after aerobic exercise training and concluded that the major factors limiting the expected weight loss from aerobic exercise were dietary compensation and a low exercise dose.20,26 currently , fewer than 30% of european adults meet exercise guidelines with a lack of time , the major barrier to regular exercise participation . accordingly , in contrast to traditional high - volume endurance training ( at 35.9 metabolic equivalent tasks ) , vigorous intensity aerobic endurance training ( at 6 metabolic equivalent tasks ) or resistance training ( muscular strength exercises using a resistance equivalent to 60%80% of the individual s one repetition maximal effort , including free weights , machines with stacked weights or pneumatic resistance , and resistance bands ) can be a time - efficient strategy to reduce body fat and improve metabolic health.27 it has been shown that in terms of weight change , when different intensities of aerobic exercise are matched for caloric expenditure or dose , both vigorous and moderate intensity aerobic trainings result in similar amounts of weight loss.28 furthermore , vigorous intensity aerobic endurance training can serve as an effective alternate to traditional endurance - based training , inducing even superior health benefits for important risk factors , including visceral fat,29 measures of glucose / insulin metabolism,30 and cardiorespiratory fitness.31 although the addition of resistance training to dietary restriction has been shown to have limited effectiveness in reducing body weight  increases in muscle mass with resistance training may off - set loss of adipose tissue the combination of diet with resistance training seems to be more effective than diet or diet with endurance training in the reduction of body mass and fat mass.32 resistance training significantly increases resting metabolic rate after a training session33 and has the power to reduce low - grade inflammation independently from weight loss in overweight / obese adults.34 muscle contractions lead to the production and release into the circulation of il-6 , which appears to have numerous biological effects , including effects on glucose and fat metabolism . acute elevations in il-6 produced by contracting the skeletal muscle downregulate the production of tumor necrosis factor- by monocytes and stimulate the release of il-1 receptor antagonist from monocytes and macrophages , thus increasing the circulating concentrations of anti - inflammatory cytokines ( such as adiponectin and il-10 ) from adipose tissue.35 in addition to exercise interventions , many studies have manipulated the macronutrient content of energy - restricted diets and have reported favorable changes in body composition with many dietary approaches.36,37 thus , reducing the calorie intake below expenditure results in a predictable initial rate of weight loss that is related to the energy deficit.38 based on recent research and meta - analytic evidence , weight loss is achieved by adherence to any diet that successfully reduces calorie intake , but adherence rates are low with most diets.39 although a low - carbohydrate diet may be associated with greater short - term weight loss , superior weight loss in the long - term has not been established.40 furthermore , very - low  carbohydrate weight loss diets may be associated with more frequent side effects , such as mood swings.41 carbohydrates are responsible for helping drive trp across the blood  brain barrier and having an effect on the brain serotonin levels.42 thus , by eating a low carbohydrate meal , the amount of trp entering the brain will be substantially lower compared with the normal situation , contributing to symptoms of depression , such as mental fatigue and low mood . in addition , trp competes with the other large neutral amino acids ( lnaa ) for transport across the blood  brain barrier . since plasma amino acids change in obese persons on hypocaloric diet , a decrease in trp lnaa ratio may further influence serotonin synthesis.43 previous work reported a decrease in plasma trp and trp / branched - chain amino acids ( bcaas ) ratio in men and women by dieting.44 as a common consequence , caloric restriction weight loss diets , especially if based upon macronutrient content that is low in carbohydrate and high in protein , lead many dieters to revert to their old eating habits , eating more and using carbohydrate - rich foods , to feel better , which is a frequent cause of weight gain , the so - called yo - yo effect.45 in a recent study,18 concentrations of essential amino acid trp decreased significantly with a caloric restriction weight loss diet , and lowest trp concentrations were observed in the group of individuals with the lowest calorie intake . the decline of trp levels can be referred to its reduced intake during caloric restriction diet as it was unrelated to the immune activation status of individuals , which remained unchanged . however , there may be gender differences in the response of plasma trp to dieting . for example , in women , but not in men , dieting significantly lowered the plasma total and free trp , indicating that dieting alters brain serotonin function in women , perhaps as a consequence of reducing the availability of plasma trp.46 calorie restriction decreases resting and total energy expenditure.47 declines in energy expenditure favoring the regain of lost weight persist well beyond the period of dynamic weight loss.48 findings from the pounds lost study confirmed a decrease in total energy expenditure , mainly contributed by a decrease in resting energy expenditure , in both men and women.49 the authors found , further , a decrease in energy expenditure from physical activity as dietary carbohydrate decreases followed by a decrease in lean body mass with a high fat diet . thus , weight loss leads to both physiological and psychological changes that promote subsequent weight regain . the path to overcome this tendency for weight regain may involve exercise and dietary strategies that improve adherence , counter the physiological and behavioral adaptations , and reestablish the balance between intake and expenditure.50 although many individuals have success in losing weight with diet , weight maintenance is a challenge , regardless of the initial modality used for weight loss . long - term adherence to restrictive diets is made difficult because of the reduction in energy expenditure that is induced by weight loss but also because of changes in the peripheral hormone signals that increase appetite.51 hence , most dieters subsequently regain much or all of the lost weight . exercise and behavioral interventions may help individuals maintain weight loss since exercise programs modify responses in a direction expected to enhance satiety and permit weight loss and/or maintenance.50 weight loss leads to compensatory changes in the homeostatic processes , including alterations in energy expenditure , substrate metabolism , and hormone pathways involved in appetite regulation that result in increased hunger and energy storage , favoring weight regain.51 in a recent review , maclean et al.52 summarized the adaptations to energy - restricted weight loss that are thought to promote weight regain . during weight loss , metabolic requirements decline as a function of ( i ) lost body mass , ( ii ) reduced consumption of food , and ( iii ) increased metabolic efficiency of peripheral tissues . neuroendocrine signals from the periphery transfer a message of energy depletion and low nutrient availability  favoring signals of hunger  to the hypothalamus and hindbrain that serve as the primary control centers for energy balance regulation . the response is that appetite increases and the expenditure of energy declines , named as the energy gap.52 to maintain the reduced weight , food intake must be restricted to the level that expended energy is suppressed or adherence to exercise must be enhanced in order to reduce the gap between appetite and expenditure . another reason why it is so hard to maintain weight loss is the evidence that weight loss may be associated with increased depressive symptoms.53 mood improvements often occur early in treatment , prior to achieving significant weight loss ; however , in the long term , personal costs of losing weight exceed the benefits . lowered trp availability during weight loss may limit the production of neurotransmitter serotonin , and this may result in mood disturbances and can , further , diminish serotonin functions ultimately leading to satiety dysregulation and increased food intake.54 it is also apparent that disturbed mood can affect the self - rewarding mechanisms of food consumption , which is likely to involve foods that are high , both in carbohydrates and saturated fats , and which in turn may promote weight gain , which could further depress mood and self - esteem over the long term.55 notably , calorie restriction diet is not only associated with a decline in the amino acid trp , the precursor of the neurotransmitter serotonin , but also with a reduction in phenylalanine , the precursor of the tyrosine dopamine pathway.18 furthermore , in obese individuals , increased activity of the immunomodulatory enzyme ido1 during immune activation results in trp depletion , and this persists in spite of significant weight reduction,56 which may slow - down the production of serotonin in the brain and further lower mood.57 following weight loss , compensatory changes include changes in the levels of circulating appetite - related hormones , such as increases in orexigenic hormones ( eg , ghrelin ) , which stimulate appetite , and decreases in anorexigenic hormones ( eg , leptin ) , which inhibit appetite , with the net result that appetite is increased.51 currently , there is a disparity in the literature concerning the influence of exercise on appetite . some researchers suggest that chronic exercise training does not induce substantial weight loss due to compensatory responses , ie , increase in appetite and food intake,58,59 while some others consistently document that exercise has no influence on appetite or ad libitum food intake.60,61 based on a recent meta - analysis , acute exercise has a trivial effect on subsequent energy intake.62 differences in lean body mass , fat mass loss , volume and duration of exercise interventions , and inclusion of different genders are likely to be factors contributing to the discrepancy . staten noted a gender difference in compensatory food intake after exercise , with an increased caloric intake in men but not in women.63 one possible reason for this is that men exhibit greater amounts of fat - free mass.64 indeed , resting metabolic rate ( largely determined by fat - free mass ) is associated with hunger , self - determined meal size , and daily energy intake and may represent a marker for appetite.65 on the contrary , the appetite and energy intake response to exercise did not differ between men and women in a recent study.66 however , women exhibit compensatory appetite , gut hormone , and food intake responses to acute food restriction - induced energy deficit but not in response to an acute bout of exercise . furthermore , there may be variability in weight - reduced subjects according to individual physiological characteristics , with clear responders and nonresponders.67 since serotonergic mechanisms may reduce body weight by accelerating the onset of satiety68 besides suppressing excessive snacking of carbohydrate - rich foods , nonpharmacological methods of raising brain serotonin during weight loss and/or maintenance could be highly useful in preventing uncontrolled weight gain . it has been shown that physical exercise ( mild stress ) normalizes levels of proinflammatory cytokines and may counteract the activation of inflammation / ido1 pathways , which may decrease the susceptibility for mood disturbances and carbohydrate craving.69 animal and human studies have shown that aerobic exercise can stimulate brain serotonin activity and trigger parallel elevations in plasma - free trp and brain trp.7072 in the primary study by chaouloff et al.70 , nonesterified fatty acid elevation increased free trp , hence its entry in the brain for serotonin synthesis and also in the liver . acute exercise seems to elevate the activity of trp 5-monooxygenase , the enzyme involved in the rate - limiting step in the synthesis of serotonin , and so leads to an increase in the concentration of serotonin in some areas of the brain , ie , the brain stem and hypothalamus.73 chronic exercise ( 30 min / d , six days per week for four weeks ) causes neural adaptations by activating not only the synthesis but also the metabolism of serotonin in the cerebral cortex.74 furthermore , salivary and serum cortisol levels in humans and corticoster - one in rats are increased by exercise,75,76 and this could induce liver trp 2,3-dioxygenase , as demonstrated in rats . the combined effects of increased liver trp and trp 2,3-dioxygenase induction can elevate serum kyn in humans77 and in rats.78 in both of these latter studies , increases in serum kyn after exercise have been shown . although exercise increased the ido1 activity of macrophages in rats,78 until now , there is no direct information on the effect of exercise on ido1 activity in humans . furthermore , the effects of elevations in pro- and anti - inflammatory cytokines by exercise on ido1 activity still remain to be examined . however , a recent study in healthy human volunteers showed a significant induction of trp breakdown as indicated by an increase in kyn / trp , which could be referred to an enhanced activity of ido1 because a significant association of kyn / trp with the immune activation marker neopterin was observed.79 trp is not only a precursor of the serotonin biochemical pathway but also the key element for the formation of nicotinamide adenine dinucleotides , nad and nadh , via the kyn pathway.80 potential signals during exercise include increased nad / nadh . with increasing exercise intensity , the cytosolic nad / nadh ratio declines as lactate accumulates.81 nad regulates the expression of sirtuins ( sirt ) and peroxisome proliferator - activated receptor- coactivator ( pgc-1 ) . enhanced sirt1 and pgc-1 activities are associated with improved mitochondrial function and exercise performance82 and protection against obesogenic feeding.83 recently , a mechanism was discovered by which overexpression of pgc-11 in muscle mimics antidepressant effects of exercise by promoting kyn aminotransferase expression . by this way , crossing of kyn through the blood brain barrier and the disruption of neural plasticity are prevented.84 during exercise , the entry of trp into the brain through the blood brain barrier is favored by increased muscle use of bcaas that inhibit trp transport into the brain and elevated plasma fatty acids , as this elevates the ratio of unbound trp to bcaa . because of the increase in plasma trp and decrease in bcaa , there is a substantial increase in trp availability to the brain , consequently leading to higher serotonin concentrations in some areas of the brain.85 serotonin plays a key role in signal transduction between neurons , and an exercise - induced increase in the concentrations of serotonin has been linked to central fatigue.86,87 although there is some evidence that bcaa supplementation may increase exercise intensity at lactate threshold,88 upper body muscle power,89 and the rate of perceived recovery after strenuous exercise,90 the association between the ergogenic effect of the bcaa intake and the reduction in serotonin production in the brain has not been tested in humans . in a recent study,91 during a half - ironman triathlon , serum amino acids concentrations were reduced by > 20% . however , neither the changes in serum - free amino acids nor the trp / bcaa ratio was related to muscle fatigue or muscle damage during the race . paradoxically , trp supplementation can decrease fatigue perception during an aerobic exercise and likewise may improve physical performance , possibly by acting via endogenous opioids.92,93 taken together , exercise increases trp availability to the brain , and these findings provide support to the hypothesis that increases in serotonin synthesis and activity might be involved in the antidepressant effect of exercise.94 however , too high serotonin levels may impair mood and increase the sensitivity to fatigue , a situation similar to the development of a transient serotonin syndrome.95 given that there is a reciprocal link in mood disorders and obesity,48 physical exercise could mitigate the biological changes that occur with weight loss ( ie , decreased metabolic mass , increased metabolic efficiency , and increased hunger and depression ) via both an increase in energy expenditure ( with the potential to generate an energy deficit ) and the induction of an anti - inflammatory environment ( with a subsequent increased release of serotonin ) . however , one has to distinguish recreational physical activity ( activity that people engage in during their free time , that people enjoy , and that people recognize as having socially redeeming values ) from repeated high level training sessions or intense endurance sports . although the recreational physical activity performed every other day maximum will exert the above - mentioned positive impact on physiological pathways and parameters , repeated high - level activities could easily turn toward a negative net effect . exhaustive physical activity significantly impacts on inflammation cascades that involve several proinflammatory cytokines , such as interferon- and down - stream biochemical pathways.96,97 this includes also alterations of amino acid profiles , eg , during and after a half iron man triathlon.91 such data fit well with the observation that intense training was associated with an accelerated trp breakdown and an increased kyn / trp.79 interestingly , intense versus moderate physical activities might also exert contrasting effects on neuropsychiatric circuits ( fig . acute and moderate physical exercises , such as noncompetitive physical exercise such as jogging , increase the activity of gtp - cyclohydrolase-1 ( gch1 ) and contribute to increased productions of pteridine derivatives.98 by contrast , in our recent study , a drop in phenylalanine and phe / tyr concentrations by exhaustive exercise was noted,79 which indicates a decreased pah activity that could be due to a decline in tetrahydrobiopterin ( bh4).99 in the same population , nitrite levels dropped ( unpublished data ) that could represent another consequence of lowered no production related to diminished bh4 . unfortunately , the direct measurements of bh4 are almost impossible because only very stringent preanalytical measures can limit the oxidative loss of this compound and practically limit its monitoring to laboratory animal or in vitro studies . still , data fit well with existing literature , which shows that acute endurance exercise may increase serotonin availability as was reflected in the periphery by increased concentration of free trp.100 the pathogenesis of negative mood is certainly multifactorial , trp and serotonin metabolism can be of some greater relevance , and implicating reactive oxygen species ( ros ) and bh4 is currently still rather speculative , although successful therapy of depression with bh4 has been reported already several years earlier.101 one may conclude that moderate physical exercise enhances the production of neurotransmitters , such as serotonin , noradrenaline , adrenaline , and dopamine , and can thereby contribute to a heightening of mood.102 however , only sports performed occasionally with two or three days interval when performed as recreational activity seems to exert this beneficial effect on general well - being , whereas intense training will rather adversely affect both mood103 and immune system function.104 other factors such as psychological stress , lack of sleep , and malnutrition can increase the risk of infection when immunity becomes suppressed . this relationship will become especially important when sports activity is performed together with a reduction in food intake as a part of a weight - loss program . after short time , calorie restriction diet was associated with a decline in essential amino acid trp in serum / plasma followed by a significant drop in phenylalanine.18 trp would further decline when the weight loss diet is combined with heavy physical exercise that induces trp degradation . it could result in deficiency of the essential amino acids that  because of their relevance for neurotransmitter biosynthesis may affect the adherence to weight loss programs . to avoid this , one might consider supplementation with trp or 5-hydroxy - trp during such periods to improve weight loss maintenance . indeed , it has been shown that acute trp supplementation while dieting could be helpful in improving mood status and preventing uncontrolled weight gain or neuropsychiatric symptoms.13 however , in healthy subjects , only large increases in brain trp levels are able to improve mood significantly , whereas relatively small increases in brain trp result in an improved mood in vulnerable subjects . so eating foods with relatively high trp content , such as turkey meat , cacao , nuts , and other seeds , is considered to increase trp availability to the brain , albeit slightly , and contributes to some mood enhancement.105 this conclusion is , however , weakened by the fact that such food usually also contains high concentrations of competing lnaa that reduces the trp : lnaa ratio and thus again limits the transport of trp to brain via the leucine - preferring l1 system.106 by contrast , in vitro observations show that antioxidant compounds are able to suppress ido1 , which supports the conclusion that healthy food rich in antioxidants could exert some additional positive effect on trp availability when trp breakdown is slowed down.107 weight loss leads to compensatory changes in the homeostatic processes , including alterations in energy expenditure , substrate metabolism , and hormone pathways involved in appetite regulation that result in increased hunger and energy storage , favoring weight regain.51 in a recent review , maclean et al.52 summarized the adaptations to energy - restricted weight loss that are thought to promote weight regain . during weight loss , metabolic requirements decline as a function of ( i ) lost body mass , ( ii ) reduced consumption of food , and ( iii ) increased metabolic efficiency of peripheral tissues . neuroendocrine signals from the periphery transfer a message of energy depletion and low nutrient availability  favoring signals of hunger  to the hypothalamus and hindbrain that serve as the primary control centers for energy balance regulation . the response is that appetite increases and the expenditure of energy declines , named as the energy gap.52 to maintain the reduced weight , food intake must be restricted to the level that expended energy is suppressed or adherence to exercise must be enhanced in order to reduce the gap between appetite and expenditure . another reason why it is so hard to maintain weight loss is the evidence that weight loss may be associated with increased depressive symptoms.53 mood improvements often occur early in treatment , prior to achieving significant weight loss ; however , in the long term , personal costs of losing weight exceed the benefits . lowered trp availability during weight loss may limit the production of neurotransmitter serotonin , and this may result in mood disturbances and can , further , diminish serotonin functions ultimately leading to satiety dysregulation and increased food intake.54 it is also apparent that disturbed mood can affect the self - rewarding mechanisms of food consumption , which is likely to involve foods that are high , both in carbohydrates and saturated fats , and which in turn may promote weight gain , which could further depress mood and self - esteem over the long term.55 notably , calorie restriction diet is not only associated with a decline in the amino acid trp , the precursor of the neurotransmitter serotonin , but also with a reduction in phenylalanine , the precursor of the tyrosine dopamine pathway.18 furthermore , in obese individuals , increased activity of the immunomodulatory enzyme ido1 during immune activation results in trp depletion , and this persists in spite of significant weight reduction,56 which may slow - down the production of serotonin in the brain and further lower mood.57 following weight loss , compensatory changes include changes in the levels of circulating appetite - related hormones , such as increases in orexigenic hormones ( eg , ghrelin ) , which stimulate appetite , and decreases in anorexigenic hormones ( eg , leptin ) , which inhibit appetite , with the net result that appetite is increased.51 currently , there is a disparity in the literature concerning the influence of exercise on appetite . some researchers suggest that chronic exercise training does not induce substantial weight loss due to compensatory responses , ie , increase in appetite and food intake,58,59 while some others consistently document that exercise has no influence on appetite or ad libitum food intake.60,61 based on a recent meta - analysis , acute exercise has a trivial effect on subsequent energy intake.62 differences in lean body mass , fat mass loss , volume and duration of exercise interventions , and inclusion of different genders are likely to be factors contributing to the discrepancy . staten noted a gender difference in compensatory food intake after exercise , with an increased caloric intake in men but not in women.63 one possible reason for this is that men exhibit greater amounts of fat - free mass.64 indeed , resting metabolic rate ( largely determined by fat - free mass ) is associated with hunger , self - determined meal size , and daily energy intake and may represent a marker for appetite.65 on the contrary , the appetite and energy intake response to exercise did not differ between men and women in a recent study.66 however , women exhibit compensatory appetite , gut hormone , and food intake responses to acute food restriction - induced energy deficit but not in response to an acute bout of exercise . furthermore , there may be variability in weight - reduced subjects according to individual physiological characteristics , with clear responders and nonresponders.67 since serotonergic mechanisms may reduce body weight by accelerating the onset of satiety68 besides suppressing excessive snacking of carbohydrate - rich foods , nonpharmacological methods of raising brain serotonin during weight loss and/or maintenance could be highly useful in preventing uncontrolled weight gain . it has been shown that physical exercise ( mild stress ) normalizes levels of proinflammatory cytokines and may counteract the activation of inflammation / ido1 pathways , which may decrease the susceptibility for mood disturbances and carbohydrate craving.69 animal and human studies have shown that aerobic exercise can stimulate brain serotonin activity and trigger parallel elevations in plasma - free trp and brain trp.7072 in the primary study by chaouloff et al.70 , nonesterified fatty acid elevation increased free trp , hence its entry in the brain for serotonin synthesis and also in the liver . acute exercise seems to elevate the activity of trp 5-monooxygenase , the enzyme involved in the rate - limiting step in the synthesis of serotonin , and so leads to an increase in the concentration of serotonin in some areas of the brain , ie , the brain stem and hypothalamus.73 chronic exercise ( 30 min / d , six days per week for four weeks ) causes neural adaptations by activating not only the synthesis but also the metabolism of serotonin in the cerebral cortex.74 furthermore , salivary and serum cortisol levels in humans and corticoster - one in rats are increased by exercise,75,76 and this could induce liver trp 2,3-dioxygenase , as demonstrated in rats . the combined effects of increased liver trp and trp 2,3-dioxygenase induction can elevate serum kyn in humans77 and in rats.78 in both of these latter studies , increases in serum kyn after exercise have been shown . although exercise increased the ido1 activity of macrophages in rats,78 until now , there is no direct information on the effect of exercise on ido1 activity in humans . furthermore , the effects of elevations in pro- and anti - inflammatory cytokines by exercise on ido1 activity still remain to be examined . however , a recent study in healthy human volunteers showed a significant induction of trp breakdown as indicated by an increase in kyn / trp , which could be referred to an enhanced activity of ido1 because a significant association of kyn / trp with the immune activation marker neopterin was observed.79 trp is not only a precursor of the serotonin biochemical pathway but also the key element for the formation of nicotinamide adenine dinucleotides , nad and nadh , via the kyn pathway.80 potential signals during exercise include increased nad / nadh . with increasing exercise intensity , the cytosolic nad / nadh ratio declines as lactate accumulates.81 nad regulates the expression of sirtuins ( sirt ) and peroxisome proliferator - activated receptor- coactivator ( pgc-1 ) . enhanced sirt1 and pgc-1 activities are associated with improved mitochondrial function and exercise performance82 and protection against obesogenic feeding.83 recently , a mechanism was discovered by which overexpression of pgc-11 in muscle mimics antidepressant effects of exercise by promoting kyn aminotransferase expression . by this way , crossing of kyn through the blood brain barrier and the disruption of neural plasticity are prevented.84 during exercise , the entry of trp into the brain through the blood  brain barrier is favored by increased muscle use of bcaas that inhibit trp transport into the brain and elevated plasma fatty acids , as this elevates the ratio of unbound trp to bcaa . because of the increase in plasma trp and decrease in bcaa , there is a substantial increase in trp availability to the brain , consequently leading to higher serotonin concentrations in some areas of the brain.85 serotonin plays a key role in signal transduction between neurons , and an exercise - induced increase in the concentrations of serotonin has been linked to central fatigue.86,87 although there is some evidence that bcaa supplementation may increase exercise intensity at lactate threshold,88 upper body muscle power,89 and the rate of perceived recovery after strenuous exercise,90 the association between the ergogenic effect of the bcaa intake and the reduction in serotonin production in the brain has not been tested in humans . in a recent study,91 during a half - ironman triathlon , serum amino acids concentrations were reduced by > 20% . however , neither the changes in serum - free amino acids nor the trp / bcaa ratio was related to muscle fatigue or muscle damage during the race . paradoxically , trp supplementation can decrease fatigue perception during an aerobic exercise and likewise may improve physical performance , possibly by acting via endogenous opioids.92,93 taken together , exercise increases trp availability to the brain , and these findings provide support to the hypothesis that increases in serotonin synthesis and activity might be involved in the antidepressant effect of exercise.94 however , too high serotonin levels may impair mood and increase the sensitivity to fatigue , a situation similar to the development of a transient serotonin syndrome.95 given that there is a reciprocal link in mood disorders and obesity,48 physical exercise could mitigate the biological changes that occur with weight loss ( ie , decreased metabolic mass , increased metabolic efficiency , and increased hunger and depression ) via both an increase in energy expenditure ( with the potential to generate an energy deficit ) and the induction of an anti - inflammatory environment ( with a subsequent increased release of serotonin ) . however , one has to distinguish recreational physical activity ( activity that people engage in during their free time , that people enjoy , and that people recognize as having socially redeeming values ) from repeated high level training sessions or intense endurance sports . although the recreational physical activity performed every other day maximum will exert the above - mentioned positive impact on physiological pathways and parameters , repeated high - level activities could easily turn toward a negative net effect . exhaustive physical activity significantly impacts on inflammation cascades that involve several proinflammatory cytokines , such as interferon- and down - stream biochemical pathways.96,97 this includes also alterations of amino acid profiles , eg , during and after a half iron man triathlon.91 such data fit well with the observation that intense training was associated with an accelerated trp breakdown and an increased kyn / trp.79 interestingly , intense versus moderate physical activities might also exert contrasting effects on neuropsychiatric circuits ( fig . acute and moderate physical exercises , such as noncompetitive physical exercise such as jogging , increase the activity of gtp - cyclohydrolase-1 ( gch1 ) and contribute to increased productions of pteridine derivatives.98 by contrast , in our recent study , a drop in phenylalanine and phe / tyr concentrations by exhaustive exercise was noted,79 which indicates a decreased pah activity that could be due to a decline in tetrahydrobiopterin ( bh4).99 in the same population , nitrite levels dropped ( unpublished data ) that could represent another consequence of lowered no production related to diminished bh4 . unfortunately , the direct measurements of bh4 are almost impossible because only very stringent preanalytical measures can limit the oxidative loss of this compound and practically limit its monitoring to laboratory animal or in vitro studies . still , data fit well with existing literature , which shows that acute endurance exercise may increase serotonin availability as was reflected in the periphery by increased concentration of free trp.100 the pathogenesis of negative mood is certainly multifactorial , trp and serotonin metabolism can be of some greater relevance , and implicating reactive oxygen species ( ros ) and bh4 is currently still rather speculative , although successful therapy of depression with bh4 has been reported already several years earlier.101 one may conclude that moderate physical exercise enhances the production of neurotransmitters , such as serotonin , noradrenaline , adrenaline , and dopamine , and can thereby contribute to a heightening of mood.102 however , only sports performed occasionally with two or three days interval when performed as recreational activity seems to exert this beneficial effect on general well - being , whereas intense training will rather adversely affect both mood103 and immune system function.104 other factors such as psychological stress , lack of sleep , and malnutrition can increase the risk of infection when immunity becomes suppressed . this relationship will become especially important when sports activity is performed together with a reduction in food intake as a part of a weight - loss program . after short time , calorie restriction diet was associated with a decline in essential amino acid trp in serum / plasma followed by a significant drop in phenylalanine.18 trp would further decline when the weight loss diet is combined with heavy physical exercise that induces trp degradation . it could result in deficiency of the essential amino acids that  because of their relevance for neurotransmitter biosynthesis , one might consider supplementation with trp or 5-hydroxy - trp during such periods to improve weight loss maintenance . indeed , it has been shown that acute trp supplementation while dieting could be helpful in improving mood status and preventing uncontrolled weight gain or neuropsychiatric symptoms.13 however , in healthy subjects , only large increases in brain trp levels are able to improve mood significantly , whereas relatively small increases in brain trp result in an improved mood in vulnerable subjects . so eating foods with relatively high trp content , such as turkey meat , cacao , nuts , and other seeds , is considered to increase trp availability to the brain , albeit slightly , and contributes to some mood enhancement.105 this conclusion is , however , weakened by the fact that such food usually also contains high concentrations of competing lnaa that reduces the trp : lnaa ratio and thus again limits the transport of trp to brain via the leucine - preferring l1 system.106 by contrast , in vitro observations show that antioxidant compounds are able to suppress ido1 , which supports the conclusion that healthy food rich in antioxidants could exert some additional positive effect on trp availability when trp breakdown is slowed down.107 the obesity epidemic that we are facing today may relate to various aspects , such as increased general availability of energy - rich foods , increased sedentary activity , and decreased occupational physical activity ( traveling to or from work or school by a means involving physical activity , such as walking and riding a bicycle).108 in theory , eating less and increasing physical activity will be able to counteract this development . however , obesity is often associated with disturbed mood , which is closely linked with the inability to lose or to stop gaining weight , and a craving for carbohydrates is manifested by many people who are overweight or are becoming so . the disturbed metabolism of trp in overweight victims might affect the biosynthesis of serotonin and could thereby increase the susceptibility for mood disturbances and carbohydrate craving , increasing the cessation probability of weight reduction programs . however , breakdown of trp triggered by proinflammatory cascades that are elicited during excessive physical exercise may again relate to the development of neuropsychiatric symptoms , such as fatigue and low mood . thus , a balance between eating habits and the optimal dose of physical activity needs to be reached .
an association between mood disturbance , the inability to lose or to stop gaining weight , and a craving for carbohydrates is manifested by many people who are overweight or are becoming so . in a recent study , we observed that low - calorie weight loss diet lowered not only levels of leptin but also levels of essential amino acid tryptophan ( trp ) significantly . 
 the disturbed metabolism of trp might affect biosynthesis of serotonin and could thereby increase the susceptibility for mood disturbances and carbohydrate craving , increasing the cessation probability of weight reduction programs . 
 alternatively , moderate physical exercise  a potent stimulus to modulate ( reduce / normalize ) proinflammatory cytokines , which may affect trp levels  could be helpful in improving mood status and preventing uncontrolled weight gain . 
 in contrast , excessive physical exercise may induce breakdown of trp when proinflammatory cascades together with trp - degrading enzyme indoleamine 2,3-dioxygenase-1 are stimulated , which may lead to neuropsychiatric symptoms such as fatigue and low mood .