polyphenols are natural aromatic compounds containing two or more phenolic hydroxyl groups that occur in remarkable amounts in tissues seeds and fruits of several angiosperms [ 13 ] . they are present in particular as defense agents facilitating protection against microbial infection and uv radiation . several of their representatives have antioxidant effects , making their biological role even more important [ 47 ] . they decrease thrombocyte aggregation   and affect apoptosis and cell proliferation through modulation of signal transduction pathways . these properties are remarkable facts in light of the who mortality statistics : nowadays cardiovascular diseases are the leading cause of death in the developed countries , causing one third of the total mortality . plant phenolics , however , are not only present in fruits and plants but they are also traceable in derived fruit products like white and red wines [ 1315 ] , determining their taste , colour and bitterness . these compounds in wines thought to account in large part for the so - called french - paradox [ 1617 ] . this expression denominates the finding that the rate of coronary heart disease mortality in residents living in certain parts of france is lower than it would be expected , despite a diet prone to such events . this strong antioxidant , neuroprotective   and cardioprotective effect of polyphenols found in wines appears in even one thousand times diluted solutions as well . phenolics occur as glycosides or as polymers , which results in equally limited digestibility and imbibitions . during the fermentation of red wine 10% alcoholic solution stabilizes the free ring compounds , so their imbibitions and digestibility increases . several authors have shown that principal component analysis of the polyphenolic fraction can separate wines according to variety . mainly hplc is used for this purpose , sometimes connected to an ms , but other techniques , such as nir and uv / vis have also been successfully used [ 1921 ] . other authors have shown that polyphenolic fingerprints can not only be used to identify the geographic origin of wines , but even their winery origin and winemaking technology used . in one of our earlier studies on hungarian red wines rather , our results showed a strong dependence of the polyphenolic composition on vintage year , but we could not distinguish among varieties based on the polyphenolic fingerprint . furthermore , it is a well known fact , that premium wine production is limited to regions climatically conducive to growing grapes with balanced composition and varietal features . some preliminary results suggest that a positive correlation exits between location of the vineyard and stilbene concentrations of wines . a similar coherence can be observed between the high levels of stilbenes and quality - oriented grape producing practices . ninety - two wines from the vintages 19962006 from three different wineries from  villny  , the southern - most wine - producing region in hungary , were analyzed for their polyphenols contents by hplc using uv - vis detection ( figure 1 ) . mean polyphenol content was highest in kkfrankos ( 463 mg / l ) and lowest in kadarka ( 258 mg / l ) . mean anthocyanin content was highest in shiraz ( 2.309 mg / l ) and lowest in cabernet sauvignon / franc cuvees ( 183 mg / l ) . the most striking fact is that kkfrankos contains relatively high mean concentrations on colourless phenolics . grp is formed under oxidative conditions during winemaking from caftaric acid and glutathione . obviously , the generally high polyphenol content of this variety prevented the formation of grp . ( 2002 ) found values between 3.1 and 107 mg / l for caftaric acid , 3.2 to 223 mg / l ( + ) -catechin , 2.4 to 143 mg / l ( )-epicatechin , 1.1 to 21 mg / l caffeic acid , and 1.2 to 13 mg / l quercetin in wines from new zealand and australia . rodrguez - delgado et al . ( 2001 ) and orbn et al . ( 2006 ) found similar concentrations in red wines from the canary islands and from the hungarian wine region our data is also in accordance with the results of californian and wines from the spanish ( 2002 ) . using canonical discriminant analysis wines could be separated by origin ( winery ) based on their polyphenol content ( figure 2 ) . these results show that different applied techniques during winemaking seem to have a much more decisive influence on the final polyphenolic composition of red wines than such things as variety and micro - climate , often also referred to as  terroir  [ 3134 ] . trans - resveratrol content of hungarian wines by grape varieties is strongly dependent on vintage and variety , which is consistent with our earlier studies . as can be seen from figure 3 , the 2002 vintage led to wines with high resveratrol content . according to winegrowing records in hungary , 2002 has been an exceptionally good year with little to no fungus pressure in the vineyard . it is well known that high fungus pressure leads to destruction of resveratrol through the stilbene oxidase of the fungus botrytis cinerea . the highest concentrations on resveratrol were found in merlot , pinot noir , kkfrankos , and cabernet sauvignon ( ref . figure 4 ) . other studies have already shown that especially wines made from grapes of the pinot family show especially high contents on resveratrol . the concentration of trans - resveratrol seems also to be dependent on winery origin as already published by bavaresco ( 2003 ) . in our studies especially high concentrations were found in the wines from the polgr winery . according to statistics there is a highly significant dependency of trans - resveratrol content on winery origin ( p = 0.004 ) , which supports the results obtained from the canonical discriminant analysis that polyphenolic content is dependent on winery origin . all polyphenol standards were from sigma - aldrich co. all reagents used were of analytical grade unless otherwise stated , acetic acid ( gradient grade ) and methanol from brenntag . all of our samples were kept between 2 and 8 c , but measured at a tempered workplace after a warming up period . elmer ( wellesley , usa ) 200 series hplc system , consisting of degasser , autosampler , pump , column oven and pda detector , was used . a chromsep ( lichrospher ) rp-18 end - capped 250  4.6 mm , 5 m ( varian , budapest , hungary ) , column was used for separation and kept at 30c . the gradient consisted of two eluents : ( a ) water / phosphoric acid ( 99.5/0.5 ; v / v ) ; ( b ) acetonitrile / water / phosphoric acid ( 50/49.5/0.5 ; v / v / v ) . separation of components was achieved as follows : the concentration of a was kept constant for 2 min , then the concentration of b was increased over 5 min to 20% , then further increased to 40% over 18 min , followed by a hold of 6 min . b was increased to 80% over 4 min and then to 100% over 5 min , followed by a hold of 2 min . some polyphenols were calculated as their respective free acids : caftaric acid , coutaric acid , fertaric acid , and grp ( grape reaction product , 2-s - glutathionyl caffeoyl tartaric acid ) . the hplc system for trans - resveratrol analysis consisted of a gynkotek ( germering , germany ) m 480 gt pump , a rheodyne 8125 ( 20 l loop ) injector and a gynkotek m 340 s uv diode array detector . a 250  4.6 mm column , packed with 6 m particle size c material was used for the separations . a chromeleon ( softron gmbh , germering , germany ) data management software system was used for the control of the equipment and for data evaluation . a multi - step gradient method was applied , using methanol  water  acetic acid ( 10/90/1 ; v / v / v ) mixture as solvent a and methanol  water  acetic acid ( 90/10/1 ; v / v / v ) mixture as solvent b at a flow rate of 1.5 ml / min . the gradient profile was : 0.018.0 min , from 0% to 40% b ; 18.025.0 min , from 40% to 100% b ; 25.027.0 min , 100% b. chromatographic separations were monitored at 306 nm . redmond , usa ) and spss ( spss inc . , chicago , usa ) . all polyphenol standards were from sigma - aldrich co. all reagents used were of analytical grade unless otherwise stated , acetic acid ( gradient grade ) and methanol from brenntag . all of our samples were kept between 2 and 8 c , but measured at a tempered workplace after a warming up period . briefly , a perkin  elmer ( wellesley , usa ) 200 series hplc system , consisting of degasser , autosampler , pump , column oven and pda detector , was used . a chromsep ( lichrospher ) rp-18 end - capped 250  4.6 mm , 5 m ( varian , budapest , hungary ) , column was used for separation and kept at 30c . the gradient consisted of two eluents : ( a ) water / phosphoric acid ( 99.5/0.5 ; v / v ) ; ( b ) acetonitrile / water / phosphoric acid ( 50/49.5/0.5 ; v separation of components was achieved as follows : the concentration of a was kept constant for 2 min , then the concentration of b was increased over 5 min to 20% , then further increased to 40% over 18 min , followed by a hold of 6 min . b was increased to 80% over 4 min and then to 100% over 5 min , followed by a hold of 2 min . some polyphenols were calculated as their respective free acids : caftaric acid , coutaric acid , fertaric acid , and grp ( grape reaction product , 2-s - glutathionyl caffeoyl tartaric acid ) . the hplc system for trans - resveratrol analysis consisted of a gynkotek ( germering , germany ) m 480 gt pump , a rheodyne 8125 ( 20 l loop ) injector and a gynkotek m 340 s uv diode array detector . a 250  4.6 mm column , packed with 6 m particle size c material was used for the separations . a chromeleon ( softron gmbh , germering , germany ) data management software system was used for the control of the equipment and for data evaluation . v / v / v ) mixture as solvent a and methanol  water  acetic acid ( 90/10/1 ; v / v / v ) mixture as solvent b at a flow rate of 1.5 ml / min . the gradient profile was : 0.018.0 min , from 0% to 40% b ; 18.025.0 min , from 40% to 100% b ; 25.027.0 min , 100% b. chromatographic separations were monitored at 306 nm . redmond , usa ) and spss ( spss inc . , chicago , usa ) .
ninety - two wines from the southernmost wine - producing region in hungary ( villny ) were analyzed for their polyphenolic content by high performance liquid chromatography ( hplc ) . 
 our results show that wine variety or vintage year could not be distinguished based on polyphenol content , but winery origin could be . 
 resveratrol concentration is mainly dependent on variety and vintage year . 
 the  human factor 
 ( i.e. , winemaking style and technology ) seems to be more decisive for the polyphenolic composition of red wines than other factors , such as variety and vintage year .