lung radiotherapy aims at delivering therapeutic ionizing radiation on lung tumor in the form of external beams from different angles while minimizing exposure to surrounding healthy tissues . errors in the lung tumor localization during therapy may lead to an undertreatment of the tumor and an overexposure of ionizing radiation to the surrounding lung tissues . lung tumor localization errors occur as the lung deforms during breathing , thereby compromising the accuracy of the radiation therapy . clinical approach to address these localization errors typically involves increasing tumor margins in radiation treatment plan and avoiding voluntary breathing variations ( e.g. , sneezing and coughing ) . a precise estimation of lung tumor position can be facilitated by a fluid structure interaction model , where the airflow inside the lungs is modeled using computational fluid dynamics ( cfd ) techniques , and the structure is modeled as a subject - specific anisotropic poroelastic medium . such an estimation of the lung tumor position will not only lead to improved adaptive radiotherapy and treatment outcomes but also lead to an improved image acquisition guidance in the future . cfd of airflow inside lungs during respiration is a challenging task due to the complexity of lung geometry , structural heterogeneity and material anisotropy , and other boundary constraints . specifically , the human lung is heterogeneous and anisotropic , with a wide range of elastic property values . this situation is further exacerbated by the presence of tumors , which significantly increase the local elastic modulus due to stiffness . several methods have been used to simulate flow and deformation in the lung , ranging from fractal theory to macroscopic . some methods allow simulation over several branching levels of the tracheobronchial tree to the alveoli level , but are computationally intensive and impracticable for near real - time application . for instance , yang et al .   documented the computation time for airflow studies in 11 airway branches to be in year 's duration . and radhakrishnan and kassinos   demonstrated the computational complexity of fluid flow solution by using a parallel cfd solver for studying the convective and diffusive particle depositions inside the lung . fluid structure interaction ( fsi ) between the airflow and the lung parenchymal region was first investigated in   by modeling the alveolar region as macro - air - sacs with isotropic elastic properties . coupling of anisotropic elastic properties for lung substructures with cfd studies has not been previously investigated . this paper describes a methodology for coupling the anisotropic elasticity with cfd analysis to effectively predict the volumetric lung displacement at different breathing phases and , by so doing , track tumor motion . the fluid structure interaction depends critically on the anisotropic nature of the subject - specific lung tissue elasticity . to incorporate the anisotropic tissue elasticity , while multizone representations have been previously investigated for arterial blood flows , it has not been used for investigating airflow inside the lungs . the usage of such a geometric representation avoided errors in airflow analyses caused by airway segmentation errors and reduced the computation time as compared to the timings previously reported in . the airflow modeling studies also demonstrated the influence of anisotropic elasticity estimated from 4dct on the resulting airflow inside lungs and the volumetric deformation . the combined usage of anisotropic elasticity and a multizone geometry representation form the key contribution of the paper . the spatially varying young 's modulus ( ym ) data is adopted from those derived based on optical flow registration of human data in a previous study . the mathematical model involves simultaneous solution of the equations governing fluid flow of air through the airway and the structural deformation of the lobe . a gauge pressure acquired from the patient using spirometry is imposed at the trachea , which in turn drives air into the lobes , and the pressure of the air inside the lobe in turn results in lung deformation during the breathing process . this flow - structure interaction ( fsi ) approach enables the prediction of the spatial velocity distribution and lung displacement over several breathing cycles . in order to investigate the impact of nonlinear elastic property , the predicted deformation with and without allowance for spatial variation in the ym anderson cancer research center , orlando , from in vivo experiments on human adult patients at different times of the breathing cycle . 4dct datasets for a human subject at 10% tidal volume intervals were taken using siemens biograph strain - gauge 64 slices ct . the 3d volumetric lung and the airways were segmented using pinnacle mbs and osirix software . the 4dct data registration algorithm was used to estimate the motion of each 3d voxel at the end - expiration 3d volume data by searching for and locating a corresponding voxel in another 3d volume at a different breathing phase . an optical flow - based motion estimation , which is based on local taylor series approximation and is further described in the optical flow literature , was used for the registration . one of the limitations of the optical flow method as applied to estimating the 3d organ motion was the low sensitivity to variations in regional motion . in order to improve the accuracy of optical flow algorithm implementation , we used a multilevel , multiresolution optical flow method , which computed optical flow between two 3d volumes at lower resolution , propagated the result to the higher resolution volume , and subsequently to the original resolution volume data . in this approach , the organ anatomy was separated into four parts : ( 1 ) lung outline , ( 2 ) large capillaries , ( 3 ) small capillaries , and ( 4 ) parenchymal region . at each level of anatomy optical flow , a multilevel , multiresolution optical flow registration was used for computing the 4d organ motion of that anatomy and integrated into the next level . the next step was to estimate the subject - specific deformation model 's kernel for the surface and the volumetric lung representations , which represents the internodal elastic interaction , and the surface lung elasticity in terms of the ym values . the volumetric lung deformation operator took as input the force applied on the voxels inside the lung and computed the subsequent change in shape . we first estimated the volumetric applied force and the displacement , which were the inputs for estimating the operator . the force applied on a lung for a given change in volume was computed using the pressure - volume curve measurement , a key pulmonary function test . this force was then spatially distributed inside the lung using the vertical gradient of pressure . such a distribution estimated the volumetric applied force .   the volumetric lung displacement was estimated using the optical flow , with euclidean distance - based interpolation of surface registration . for both the surface and volumetric lung deformation , a heterogeneous green 's function ( gf ) based formulation was considered . the structural and functional constants estimated for the surface lung dynamics were specifically used for the volumetric lung dynamics . the gf for the volumetric lung was reformulated in the spectral domain using a hyper spherical harmonic ( hsh ) transformation . upon simplification , the hsh coefficients of the displacement were represented as a product of the hsh coefficients of the applied force and the deformation operator . the formulation at this stage was mathematically ill - posed since the dimension of the hsh coefficients was higher than the volumetric displacement and the applied force . local isometricity was assumed at each volumetric point obtained using the structural and functional constants associated with each voxel . the constraints were computed from the values associated with the lung surface points using a surface lung deformation model . this approach reduced the dimensionality of the deformation operator , making the formulation well - posed . thus for known values of the applied force and displacement , the hsh coefficients of the operator were estimated , and the ym value of each volumetric point was computed . the mathematical model involves solution of the coupled poroelastic flow - structure interaction equation with nonhomogeneous and anisotropic tissue properties . this coupled field approach required the solution of the richard 's equation   for the local lung pressure and velocity distributions , given by 
 ( 1)pt=[k(p+g)]t(ux+vy+wz ) , 
 where  and k represent the porosity and permeability of the tissue , respectively ,  and  represent the compressibility and viscosity of air , respectively , p is the local pressure ( pore pressure ) ,  is air density , and u , v , and w are the three components of the deflection ( deformation ) vector for the tissue . note that the above equation for pore pressure has already incorporated the darcy equation for gas flow through the tissue skeleton . this equation was coupled to the lung elastic deformation by the presence of the dilatation ( final term ) in the above equation . this term was supplied by solving the elastic deformation field , u , from the following poroelastic version of the navier 's equation : 
 ( 2)g2u+g12v(u)=pf , 
 where g and v are the tissue shear modulus and poisson ratio , respectively , and f is an external body force term that can include thermal effects as desired . note that in ( 2 ) g represents anisotropic shear modulus . assuming orthogonal anisotropy the value of g is allowed to vary in the xy , yz , and xz planes . the shear modulus is related to the ym through the standard relation ( g = ym/[2(1 + v ) ] ) in each direction . together the previous two equations provide the full description of the coupled lung flow problem . solution of these equations is accomplished for subject - specific patient lung geometries using adina computational code . the ct scans at the end - expiration stage are first segmented and used to generate the three - dimensional ( 3d ) geometry utilizing the mimics computer code . the 3d meshes obtained are then remeshed by means of the 3-matic framework   for numerical computation . the resulting geometry reconstructed for the right lung based on this airway structure of the lung , the permeability ( k = r/8 , in which r is the branch radius ) should decrease significantly from the main central branches to the tip branches as the branch diameter progressively decreases . correspondingly , the airflow velocity in the primary central branches is significantly higher than that in the peripheral branches . a relatively fine volume numerical mesh size will therefore be required in the core region to reflect the relatively higher pressure , velocity , and stress gradient there compared to the outer layers . since proper representation of the grid structure is critical to numerical stability , the multibranching lung structure is approximated within the context of the poroelastic model used here , as a multizone structure shown in figure 3 , that permits application of different grids and , if needed , different property values . it can be seen that the multizone geometry representation is similar to the hyperspherical formulation used for representing the ym values of lung substructures . for instance , a normalized airway branch radius can be converted to permeability values associated with each voxel . this permeability is then allowed to vary from the core ( inner shell ) to the peripheral layers ( outer shell ) using the same hyperspherical parameterization . it should be noted that both figures 3 and 4 represent cut - out views of the lobe in order to visualize the multi zone structure and the numerical grid employed . phasic pressure with a period of 4  s is imposed at the inlet to the lobe as illustrated in figure 5 . the amplitude of the pressure waveform was prescribed based on spirometry studies acquired from m.d . the property data ( beside the ym ) were assumed from those established in previous studies . the poisson ratio v in the poroelastic governing equation was assumed to be 0.4 , which falls within the range ( 0.250.47 ) suggested in previous studies [ 22 , 23 ] . preliminary studies indicated that the deformation is little affected by the permeability k over the range ( 0.010.1 ) as expected . the anisotropic ym adopted from a previous study based on optical flow registration patient data ranged from 10  pa to 500  pa . the high ym values correspond to either the tumor location where the structure is rigid or the main trachea wall where the tissue is thick and rigid . figure 6 shows representative color - coded ym distribution on a 2d slice of lobe obtained from optical flow registration and used for the anisotropic elasticity calculations in this paper . the predicted lung deformation simulated using the flow structure interaction at different breathing phases is plotted in figures 7 and 8 for the linear and anisotropic ym cases , respectively . the upper part of each figure is a cut - off view to illustrate the evolution of selected layers from the initial state over the specified duration . figure 7 shows that with linear elasticity , the layers expanded monotonically in all directions . on the other hand , figure 8 shows that the case with anisotropic elasticity exhibits both directional deformation as well as expansion . three nodes or landmarks on the lung surface , marked a , b , c in figures 7 and 8 , were monitored , and their displacements along the x , y , and z coordinate directions were analyzed . landmark a was at the top surface of the lobe , b was at the outer surface near the rib cage and close to the midpoint along the craniocaudal axis , and c was at the interior surface of the lobe . figure 9 shows the x , y , z displacements of the monitored nodes a , b , and c over the first respiration cycle for both linear and anisotropic elasticity . the predicted displacements with linear and anisotropic ym are quite distinct . the displacement profiles for the linear ym case , which are represented by the dash lines , are generally sinusoidal with a symmetric axis at t = 2  s , corresponding to the sinusoidal pressure waveform imposed at the inlet to the lobe . on the other hand , the displacement trajectories for the anisotropic ym case , which are represented by the solid lines in each figure , a is located at t = 2.4  s , which lags the peak inlet pressure at t = 2.0  s by 0.4  s. the peak z displacement for node a is 0.2  s ahead of the peak inlet pressure . a similar hysteresis phenomenon is observed for the anisotropic ym case at nodes b and c. in addition , the peak displacements in the x direction for nodes a and b in the anisotropic ym model are nearly 3 times the displacements in the linear ym model . these results clearly show that the effect on deformation of anisotropic lung elasticity could be significant . in order to further examine the effect of anisotropicity , the calculations were continued for additional breathing cycles . figure 10 shows the x , y , z displacements of nodes a , b , and c for linear ym over 6 breathing cycles . the result indicates that all the peak displacement values occur at the midpoint of each cycle , that is , at t = 2  s , 6  s and 10  s. the displacements at the end of each cycle are nearly negligible . it is worth noting that in the consensus of the result presented in a previous figure 9 , the displacement profile observed with linear elasticity follows closely the input pressure wave pattern . the corresponding results over 6 breathing cycles utilizing anisotropic elasticity are presented in figure 11 . the observed hysteresis time for the peak wave appears to be a fixed value for each monitored location . for example , the predicted peak wave of the x displacement lags the peak pressure inlet by 0.4  s , 0.3  s , and 0.2  s for nodes a , b , and c , respectively . the hysteresis time is also found to be dependent on the geometric location of the monitored point in the lobe . in order to further examine the peak variation in the anisotropic case , the calculations are extended to 12 respiration cycles , and the results are plotted in figure 12 . the results indicate that the x displacement magnitude profile for the monitored node a reaches local peaks at the 2nd , 6th , and 10th cycles , corresponding to a period of 16  s. the y and z displacement profiles reach their local maxima at the 4th , 8th , and 12th cycles , which also correspond to a period of 16  s. a similar periodic pattern is observed for node c while the trend in node b is not quite so distinct , perhaps because the latter was located near the symmetry point along the craniocaudal axis . figure 13 summarizes the results presented above by tracing the trajectories of monitored point a over 6 breathing cycles with both linear ( figure 13(a ) ) and anisotropic elasticity ( figure 13(b ) ) . hysteresis is clearly evident in the anisotropic result as exemplified by the significant differences in the trajectories of the monitored point over successive breathing cycles . the results for nodes b and c exhibit similar trends and are not presented here for brevity . studies were conducted to verify the accuracy of the lung deformation using the cfd - based flow analysis . numerical accuracy was first tested by repeating the calculations for a set of grid numbers below and above the ones chosen for the above results . the results were found to be essentially independent of grid number ( and , correspondingly , grid size ) beyond the ones chosen for the results . the motion of the landmarks during the cfd simulation was documented and compared with the displacement observed in the 4dct dataset that was used to generate the 3d geometry . table 1 tabulates the mean target deformation error ( tde ) for the displacement obtained using the isotropic and anisotropic ym values . it can be seen that a better accuracy was obtained for the anisotropic case as compared to the isotropic case . the maximum error for both the validations was with 3  mm for the anisotropic case , which is within the clinically acceptable accuracy range . this validation shows that using anisotropic ym values for modeling airflow inside lungs and the subsequent fluid structure interaction can be achieved within clinically acceptable accuracy range using anisotropic elastic values . the effect has been investigated of using subject - specific anisotropic lung elasticity for studying the airflow - induced lung deformation during radiotherapy . the lung geometry at the end - expiration was reconstructed from 4dct dataset of patients with non - small - cell lung cancer ( nsclc ) . the subject - specific tissue elasticity was obtained from the 4dct using an inverse deformation analysis . the airflow - tissue interaction model involved solving the coupled equations governing fluid dynamics of airflow inside the lungs and the associated structural deformation of the lung , subject to appropriate boundary conditions . the major findings of the study may be summarized as follows.the local anisotropy in the elasticity of the lung substructures has a significant impact on the airflow inside lungs.anisotropic ym of lung substructures produces a hysteresis effect on the predicted spatial lung displacement relative to the pressure waveform imposed at the inlet to the lung . the hysteresis time spatially varies from one 3d location to another inside the lungs . the local anisotropy in the elasticity of the lung substructures has a significant impact on the airflow inside lungs . anisotropic ym of lung substructures produces a hysteresis effect on the predicted spatial lung displacement relative to the pressure waveform imposed at the inlet to the lung . the above findings have profound implication in the optimization and targeting of radiation to tumor in radiotherapy . the presence of tumor ( focal or distributed ) in the lung substructures may alter the anisotropism in the lung poroelasticity and , hence , as this study has indicated , significantly affects the resulting spatiotemporal displacement and deformation of the lung . in addition , changes in the tumor regression may lead to changes in the overall anisotropism of the tissue elasticity and subsequently the hysteresis . the net result is an evolving tumor location that is significantly distinct in shape from one breathing signal to another . the model presented here has demonstrated the capacity to fully represent and quantify such detailed motion of any location in the lung utilizing subject - specific tissue elasticity for lung substructures . the proposed method of geometry representation took approximately 24 hours to finish the breathing simulation , which is improvement to previous run time calculations using a full airway geometry . future work would focus on using high performance graphics units to accelerate the calculations so that the process can be finished in much lesser timeframe . results section discusses validation results for the fluid structure interaction , which showed that anisotropic ym values were effective in modeling the airflow inside the lungs . validating the airflow inside the lungs for different breathing patterns and the subsequent interaction studies will be a key part of our future work . such a study would involve the use of tracking landmarks in the lung anatomy by means of 2d cine mri imaging that can acquire the 2d lung snapshots in a given plane in real - time . in addition , using 4d gated mri imaging , we can validate the volumetric lung deformation and the fluid structure interaction studies taking into account physiological factors such as tumor regression and day - to - day breathing changes . such cardiac motion can be acquired using 2d cine mri imaging in addition to 4d imaging acquired for treatment purposes and modeling the lung deformation subject to being constrained by the cardiac motion .
lung radiotherapy is greatly benefitted when the tumor motion caused by breathing can be modeled . 
 the aim of this paper is to present the importance of using anisotropic and subject - specific tissue elasticity for simulating the airflow inside the lungs . 
 a computational - fluid - dynamics ( cfd ) based approach is presented to simulate airflow inside a subject - specific deformable lung for modeling lung tumor motion and the motion of the surrounding tissues during radiotherapy . 
 a flow - structure interaction technique is employed that simultaneously models airflow and lung deformation . 
 the lung is modeled as a poroelastic medium with subject - specific anisotropic poroelastic properties on a geometry , which was reconstructed from four - dimensional computed tomography ( 4dct ) scan datasets of humans with lung cancer . 
 the results include the 3d anisotropic lung deformation for known airflow pattern inside the lungs . 
 the effects of anisotropy are also presented on both the spatiotemporal volumetric lung displacement and the regional lung hysteresis .