pigmentary glaucoma ( pg ) and pigment dispersion syndrome ( pds ) are subtypes of secondary open - angle glaucoma ( oag ) . both represent a spectrum of the same disease characterized by excessive pigment liberation throughout the anterior segment of the eye and account for 1.0%1.5% of glaucoma cases in the western world.1 although rare , pg and pds tend to occur at a younger age than primary oag . average age at onset is between the third and fifth decades and advanced glaucomatous optic - nerve damage at time of presentation is not uncommon . the underlying mechanism responsible for pg and pds is friction of the posterior iris surface against the anterior lens zonular bundles during physiological pupil movement caused by a concave iris contour ( posterior bowing ) . this leads to a disruption of the iris pigment epithelial cell membrane and release of pigment granules . clinical features include the presence of krukenberg s spindle , iris transillumination defects , and trabecular meshwork pigmentation.1,2 posterior bowing of the mid peripheral iris is of particular importance in the etiology of pds , as it results in a close irido - zonular contact that can be confirmed by ultrasound biomicroscopy ( ubm ) and anterior - segment optical coherence tomography ( as - oct ) . in pg , for example , it has been demonstrated that the insertion of the iris into the ciliary body is more posterior.3 this has been suggested to increase the irido - zonular contact and induce a more significant clinical picture.3 histologic studies revealed that areas of disrupted iris pigment epithelium had a radial distribution corresponding to the peripheral iris transillumination defects,4,5 supporting the concepts originally proposed by campbell.4  as - oct  is a noninvasive technique allowing the visualization and measurement of the anterior ocular structures . anterior - segment parameters may be quantitatively assessed using a noncontact method with the patient in a sitting position.6 therefore , it has proven a valuable tool for visualizing and monitoring anterior - chamber ( ac ) structures in several diseases including glaucoma . peripheral laser iridotomy ( pi ) relieves the reverse - pupillary block by preventing posterior concavity of the iris , which probably induces pigment dispersion.4,7 specific changes in the anterior - segment morphology after pi can be quantified by as - oct.8 the ocular response analyzer ( ora ) has been developed to measure the in vivo biomechanical properties of the cornea . it indirectly assesses corneal hysteresis ( ch ) , which may be a measure of ocular viscous properties reflecting the tissue capacity to absorb and dissipate energy , and corneal resistance factor ( crf ) , which may be a measure of overall ocular rigidity . a number of studies found that low ch is associated with certain forms of glaucoma and that progressive visual - field loss in glaucoma may be associated with low ch.9 as cornea and sclera are continuous collagenous sheaths of the eye , they are built from similar extracellular matrix constituents,10 suggesting that biomechanical properties of the lamina cribrosa also correlate with the corneal characteristics evaluated.11 corneal properties may be related to the resistance of the optic - nerve head12 and are therefore of interest in glaucoma patients . to date , as far as we are aware , the biomechanical properties of pg eyes have not been studied in detail . the objective of this study was therefore to evaluate biomechanical properties of pg eyes and the predictive value of these properties with regard to changes after pi . this study was a prospective investigation conducted in a german university - affiliated glaucoma center . inclusion criteria were pg due to pds with documented backward bowing of the iris and glaucomatous alteration of the optic - nerve head with an elevated cup  disc ratio ( median = 0.8 ) . in one of the nine patients included , only one eye fulfilled the inclusion criteria , as pg was unilateral . the study was approved by the institutional review board of the department of ophthalmology of ludwig - maximilians university , munich , germany , and was in accordance with the declaration of helsinki . all study participants were caucasian phakic patients and underwent a full ophthalmic examination , including objective and subjective refraction , slit - lamp biomicroscopy , intraocular pressure ( iop ) measurement with goldmann applanation tonometry , gonioscopy , dilated fundus examination by indirect ophthalmoscopy , and central corneal thickness measurement prior to and after pi ( for patient details see table 1 ) . a 30 - 2 humphrey field analyzer ( hfa ii - i ; carl zeiss meditec , munich , germany ) with peridata software for windows , version 2.3 ( peridata software gmbh , huerth , germany ) was employed for visual - field testing . perimetric status was assessed with a mean deviation of 7.08.0 and a pattern standard deviation of 5.34.3 . laser pi was performed between 11 and 1 oclock using a neodymium - doped yttrium aluminum garnet laser ( single dose of 3.211.2 mj , 217 shots ) . patency of pi was ensured by pigment flowing into the ac directly after laser application . at follow - up , the iridotomy was confirmed to be patent and adequate in size via positive transillumination and change of anterior - segment configuration on as - oct . baseline and follow - up visits were all scheduled in the morning to avoid bias due to diurnal variation upon iop . in our study , as - oct ( sl - oct ; heidelberg engineering gmbh , heidelberg , germany ) was performed under standard illumination conditions ( approximately 10 lux ) in a non - accommodative state before pi and 3 months afterward . three images of each quadrant ( first superiorly , then inferiorly , temporally , and nasally ) were taken and analyzed using heidelberg eye explorer software version 5.32 ( heidelberg engineering gmbh ) . whenever necessary , the upper lid was lifted up for complete imaging of the chamber angle . optical coherence tomography scans were acquired with the protocol anterior segment single 0 to 180 ( 8  16 mm [ depth  width ] , with 256a - scans per line ) and the scan line was manually adjusted to bisect the pupil . the scleral spur was marked manually and the angle opening was determined by a single examiner ( ak ) blinded to the status of the eye ( figure 1 ) . parameters assessed by as - oct included angle width in all four quadrants ( 3 , 6 , 9 , and 12 oclock meridians ) and ac volume . in our study , as - oct ( sl - oct ; heidelberg engineering gmbh , heidelberg , germany ) was performed under standard illumination conditions ( approximately 10 lux ) in a non - accommodative state before pi and 3 months afterward . three images of each quadrant ( first superiorly , then inferiorly , temporally , and nasally ) were taken and analyzed using heidelberg eye explorer software version 5.32 ( heidelberg engineering gmbh ) . whenever necessary , the upper lid was lifted up for complete imaging of the chamber angle . optical coherence tomography scans were acquired with the protocol anterior segment single 0 to 180 ( 8  16 mm [ depth  width ] , with 256a - scans per line ) and the scan line was manually adjusted to bisect the pupil . the scleral spur was marked manually and the angle opening was determined by a single examiner ( ak ) blinded to the status of the eye ( figure 1 ) . parameters assessed by as - oct included angle width in all four quadrants ( 3 , 6 , 9 , and 12 oclock meridians ) and ac volume . corneal biomechanical properties were measured before and 3 months after pi by the ora ( reichert ophthalmic instruments , depew , ny , usa ) . the details have been described previously9,1318 briefly , the difference between the inward and outward motion applanation pressures is the ch and is an indicator of viscous damping in the cornea . the  crf  is a measure of the cumulative effects of both the viscous and elastic overall resistance of the cornea . biomechanical and pressure values recorded included ch , crf , corneal compensated intraocular pressure ( iopcc ) , and goldmann - equivalent intraocular pressure ( iopg ) calculations of iop - corrected ch and crf were conducted according to sprl et al11 with references of iop = 18 mmhg and central corneal thickness = 540 m , taking into account that the biomechanical properties of the eye are pressure dependent.19 the parameters used for analysis were then ch corrected ( corr ) and crfcorr . out of a minimum of four consecutive ora measurements taken , mean values were calculated . statistical analysis was performed using spss ( v 19.0 ; ibm corporation , armonk , ny , usa ) , samplepower for spss ( v 3.0 ; ibm corporation ) , and microsoft excel for windows 2007 ( microsoft corporation , redmond , wa , usa ) . smirnov testing for normal distribution and calculation of correlation coefficients ( spearman s rho ) . data were also fitted to multiple linear regression analyses ; t - based 95% confidence intervals for the regression coefficients were used . for all tests , p<0.05 was considered to be statistically significant . for the number of eyes included into this study , power analysis evaluating changes of chamber angles and chcorr resulted in a statistical power of > 85% . a significant flattening of the ac angle in all eyes was imaged on as - oct ( figure 1 displays a representative example ) . the decrease in the chamber angle after pi was highly significant in all four quadrants ( p<0.001 ) . the mean angles before pi were 57.09.6 , 56.18.1 , 55.89.3 , and 55.89.8 , and 44.15.2 , 46.86.4 , 44.25.5 , 44.75.8 after pi for the temporal , nasal , superior , and inferior quadrants , respectively ( figure 2 ) . the effect on chamber - angle reduction after pi was highest in the temporal quadrant , followed by the inferior , superior , and nasal quadrants (  = 12.9 , 10.9 , 10.6 , and 9.3 , respectively ) . the mean volume of the ac decreased significantly from 213.136.4 mm before to 187.023.4 mm after pi ( p<0.001 ; figures 3 and 4 ) . the corneal biomechanical properties did not change after pi ( table 2 , student s t - test p=039 and p=0.44 for chcorr and crfcorr , respectively ) . chcorr crfcorr was 0.761.77 mmhg before and 0.540.58 mmhg after pi ( p=0.97 ) . in correlation analysis , there was a strong and significant correlation of the superior and inferior angle width with pre - pi chcorr ( spearman s rho=0.553 and 0.615 , p<0.05 , respectively , figure 5a and b ) . for the temporal and nasal angles , correlation with pre - pi chcorr was not significant ( r=0.460 and r=0.474 , p=0.06 , respectively ) . multiple regression analyses were performed to analyze the impact of corneal biomechanics on as changes . in the modeling , however , when modeling for the preoperative inferior angle as the dependent variable , the parameters chcorr , crfcorr , age , and the spherical equivalent could explain 56.4% of the variance , with chcorr being the only significant predictor ( p=0.04 ; table 3 ) . the mean iop decreased , yet not significantly , from 15.04.2 mmhg to 13.22.3 mmhg after pi ( p=0.13 ) . in eyes with pds / pg , pi equalizes the pressure between the anterior and posterior chamber , flattens the iris , and thus reverses posterior iris bowing . ac angle parameters imaged by as - oct are proven significantly greater in eyes with pg20 and an effect after pi is noted.21 our results show a highly significant reduction of the ac width after pi visualized by as - oct in all meridians studied . hereby , the largest effect was observed in the temporal quadrant and therefore not in the region where the pi was performed . the ac volume also decreased significantly after pi in pg eyes , which is in accordance with previously published results.22 as - oct is an emerging method for detailed imaging of as structures in glaucoma . previous studies including patients with primary angle closure have been able to illustrate significant widening of the ac angle after pi using as - oct.8 it is a promising technology with a high reproducibility rate.23,24 image resolution and results obtained are comparable to those obtained with ubm.8 the visualization of the scleral spur is even sharper25 and precise evaluation of internal horizontal ac dimension can be considered superior to ubm analysis.20 a major finding of our study is that corrected ch is positively correlated with ac morphology in pg eyes , in particular with the superior and inferior ac angles . pds eyes are reported to have a flatter22 and larger cornea.3,26 the iris tissue in pds eyes may be weaker or more flaccid and therefore more prone to stretching than in non - pds eyes,27 and it can be assumed that with increasing hysteresis of the cornea these flaccid characteristics of the iris might be more pronounced . in eyes with long - lasting oag , ch has been repeatedly reported to be lower than in healthy eyes , resulting in decreased damping properties and increased stiffness of the continuous collagenous sheaths of the eye , setting the focus of glaucoma pathogenesis in the region of the lamina cribrosa and optic - nerve head.10,12 the values of ch and crf in our study population are within the range of healthy controls.11 one explanation for this finding might be the younger age of patients with pg ( mean 42 years in our sample ) , as oag patients are generally older and ch decreases with age . further , the different ch values in oag and pg could be due to the different glaucomatous pathogenesis . while lower ch in oag indicates more advanced glaucoma and eyes with low ch are more sensitive to iop elevations,28,29 in pg the opposite might be true : a higher ch compared with oag patients is associated with larger ac angles and more severe bowing of the iris , and these eyes might have a higher risk of severe glaucomatous optic - nerve damage . a difference between ch and crf ( chcorr  crfcorr ) of zero or a slightly negative value stands for equilibration of viscous ( ch ) and total elastic ( crf ) components.11 in this series , the therapeutic effect of pi remains controversial : while preliminary success has been reported by campbell and schertzer30 and gandolfi and vecchi,31 who reported a decreased incidence of ocular hypertension , other groups such as reistad et al32 or , most recently , scott et al could not find a benefit of laser pi in preventing progression from pds to pg.33 scott et al estimated iris concavity subjectively and did not measure changes of the anterior segment objectively . accommodation , globe deformation caused by blinking , and physical exercise have been shown to increase iris concavity.1,3436 these risk factors for an increase of iop in pds eyes decrease with age due to anatomical changes of the eye.1 inclusion of older patients might therefore bias success of pi . although the therapeutic effect of laser pi has not been yet validated in a prospective , representative clinical setting , it could be assumed that patients with pg , large ac angle , and higher ch could especially benefit from laser pi . in our study , patients with these characteristics showed a highly significant change in iris concavity . aptel et al observed a significant decrease in iop after pi.22 the iop in our sample decreased only marginally . local and systemic therapy differed in some patients before and after the procedure , which might have effected the change . the small number of eyes included has resulted in high standard deviations compared with those of studies with larger patient collectives . we do acknowledge the possibility of statistical influence when including both eyes of a subject . yet , statistical power remained > 85% when including only one eye per subject compared with all 17 eyes , indicating no relevant influence for the purposes of this study . as - oct is helpful for the diagnosis of anterior - segment changes in pg as well as for the follow - up of patients with pg during medical treatment and after pi or surgical interventions.20 we observed highly significant changes of the anterior - segment via as - oct . , biomechanical properties in these eyes seem to be higher than in other glaucoma subgroups . due to embryogenesis , anatomical characteristics in corneal tissue measured in vivo via ora might lead to conclusions of biomechanical features of the iris . further studies including a larger number of treated eyes and possibly differentiating between pds and pg are warranted . longitudinal data concerning the influence of ch on the progression of pg would be of great interest . pi in eyes with pg results in a highly significant reduction in the ac angles and volume as visualized by as - oct , with the largest effect seen in the temporal quadrant . ch is strongly positively correlated with the superior and inferior preoperative ac angles , emphasizing the importance of the biomechanical properties of the cornea for glaucoma pathogenesis in pg , but corneal biomechanical properties can not predict pi - related ac changes .
purposethe aim of the study reported here was to evaluate characteristics of the anterior - segment via anterior - segment optical coherence tomography ( as - oct ) and corneal biomechanical properties using an ocular response analyzer and their changes by peripheral laser iridotomy ( pi ) in patients with pigmentary glaucoma ( pg).materials and methodsseventeen eyes with pg were included consecutively . 
 as - oct and ocular response analyzer measurements were taken before and 3 months after pi . 
 baseline morphology and change in morphology were analyzed by correlation and multiple linear regression analysis . 
 the main parameters assessed were anterior - chamber ( ac ) angles and volume as well as corneal hysteresis ( ch ) and corneal resistance factor.resultsac angles were found to have decreased significantly in each quadrant after pi ( p<0.001 ) , with the highest effect seen in the temporal quadrant , which decreased from 57.09.6 to 44.15.2 (  standard deviation ) . 
 mean ac volume decreased significantly from 213.136.4 to 187.023.4 mm3 ( p<0.001 ) . 
 ch and corneal resistance factor did not change after pi . 
 ch was found to correlate with the preoperative superior and inferior angle width ( spearman s rho 0.553 and 0.615 , respectively , p<0.05 ) . 
 biomechanical parameters showed no predictive value on the change of ac angles or volume.conclusionpi in eyes with pg results in a highly significant reduction in the ac angles and volume as visualized by as - oct , with the largest effect seen in the temporal quadrant . 
 ch is strongly positively correlated with the superior and inferior preoperative ac angles , emphasizing the importance of the biomechanical properties of the cornea for glaucoma pathogenesis in pg , but corneal biomechanical properties can not predict pi - related ac changes .