the database from pathlab in cairo , egypt was searched for cases of amyloidosis between january 2012-may 2015 . a total of 161 formalin fixed paraffin embedded kidney biopsies with amyloidosis were identified during this time period . all cases were processed at pathlab by light and immunofluorescence microscopy using routine techniques , previously described . briefly , kidney biopsies were fixed in buffered formalin , dehydrated in graded alcohols , and embedded in paraffin using standard techniques . serial 3  m - thick sections were cut and treated with hematoxylin and eosin , jones methenamine silver , masson trichrome , and periodic acid - schiff reagent . a congo red stain was performed in all cases in addition to serial sections for staining . immunoperoxidase stains for aa ( dako , carpenteria , ca , usa ) and lect2 ( r&d systems , minneapolis , mn , usa ) as well as paraffin immunofluorescence for  and  ( both from dako , carpenteria , ca , usa ) were performed in all cases . detailed methods for the aa and lect2 staining procedures are provided in supplementary tables 1 and 2 , respectively . cases that were not able to be typed using these stains were sent to mayo clinic for typing of the amyloidosis by mass spectroscopy as previously described ( figure 1 ) . the extent of amyloid deposition in different compartments of the kidney was evaluated by reviewing the congo red stained slides . glomerular amyloid deposits were scored with a semi - quantitative scale as follows : 0 , absent ; 1 , amyloid deposits affecting ~25% of the glomerulus ; 2 , 2550% of the glomerulus , and 3 , > 50% of the glomerulus . the extent of amyloid deposition in the cortical and medullary interstitium was scored as : 0 , absent ; 1 , 25% of parenchyma involved ; 2 , 2550% , and 3 , > 50% . the extent of amyloid deposition in arteries was scored as : 0 , absent ; 1 ; mild , 2 ; moderate ; and 3 , severe . the results of the amyloid type in this case series are shown in table 1 . aa patients had a mean age of 44.9 years and al patients had a mean age of 56.1 years . the etiology of the aa amyloid was unknown in the majority of cases though , among the 56 cases , there were 9 cases with familial mediterranean fever and 2 cases associated with rheumatoid arthritis . the medullary interstitium was the compartment with the least involvement and , when present , the deposits were often sparse . the amyloid deposits showed bright red congophilia in all cases with green birefringence upon polarization . among the alect2 cases ten of the cases had additional histologic diagnoses including five cases with arterionephrosclerosis ( 14% ) , two cases with diabetic glomerulosclerosis ( 6% ) , and one case each of membranous glomerulopathy ( 3% ) , acute tubular injury ( 3% ) and interstitial nephritis ( 3% ) . most cases were typed by immunohistochemical staining without mass spectroscopy as there were no cases with more than one antibody positive in the amyloid deposits . mass spectroscopy was utilized for amyloid typing in 14 cases due to either weak positive staining in the amyloid deposits by the lect2 stain ( nine cases ) or no staining by any of the antibodies ( five cases ) . all nine cases with weak lect2 staining had an amyloid type that matched by mass spectroscopy . the five cases with negative staining included two cases of alect2 , one case of fibrinogen a -chain amyloidosis , and 2 cases of al  amyloidosis . alect2 was found to be significantly more common in this population than has been described in other case series of renal amyloidosis . the largest renal amyloid case series reported in the united states ( 474 cases ) showed al to be the most common amyloid subtype identified , representing 85.9% of amyloidosis cases followed by aa at 7% and alect2 at 2.7% . a second large renal amyloid study in the us ( 414 total cases ) showed similar findings with al representing 83.3% of amyloid cases . however , focusing on the amyloidosis type in renal biopsies that came from the southwest us , a very different picture emerged with 54% of cases showing alect2 and 41% al . this is likely due to the fact that there is a much higher population of patients with mexican ethnicity in this region of the country . renal amyloid case series from germany and switzerland have also shown al to be the most common type of amyloidosis at 53.2% but , similar to the egyptian data , had a much higher burden of aa at 40.3% . no cases of alect2 were reported in these european series ; however , it was not specifically tested for . by comparison , our current egyptian series shows aa amyloidosis to be the most common at 48% with alect2 representing the second most common at 31% followed by al at 20% . it is likely that other populations with a high disease burden of alect2 will emerge as awareness of the disease increases . the clinicopathologic findings in egyptian alect2 patients are similar to those previously described in the literature . the egyptian cohort was slightly younger at 59 years than the previously described us cohorts , which were primarily comprised of hispanics and had a mean age of 69 years . this egyptian cohort also includes the youngest person reported with alect2 in the kidney to date at the age of 30 years . aa and al patients in the egyptian cohort were also younger than the previously described us cohorts . this could be in part due to a shorter life expectancy in egypt compared with the us ( 71 vs 79 years ) resulting in underrepresentation of elderly patients with amyloidosis in the egyptian cohort . the morphologic pattern of deposition in the kidney was similar to that previously described in other large case series with amyloid predominantly affecting the cortical interstitium ( table 2 ) . the strong predilection for certain ethnic groups would suggest a genetic component but , thus far , no disease causing variants have been identified . it is hypothesized that overproduction of alect2 by the liver leads to deposition in the parenchyma of various organs . all alect2 patients with sequencing of the lect2 gene have been found to be homozygous for the g nucleotide in a non - synonymous snp at position 172 ( snp rs31517 ) . this polymorphism is common with an allele frequency of 0.51 in patients of mexican ancestry . the overrepresentation of this common polymorphism in patients with alect2 indicates that it is necessary for disease to develop but not the sole etiology of the disease . an invasive procedure such as renal biopsy is the only way to make the diagnosis of alect2 . deposition has previously been reported in the multiple organs including the spleen , liver , prostate , and pancreas . however , there have been no reports of deposition in the skin or fibroadipose tissue to suggest that a less invasive diagnostic procedure such as fat pad aspirate or skin biopsy would be useful . alect2 most commonly presents with slowly progressive renal failure and we have shown that they often have comorbid illnesses such as diabetes mellitus and hypertension . since slowly progressive renal failure with bland urine sediment is not typically an indication for renal biopsy , particularly when the patient has comorbid illnesses that are known to lead to renal disease , we believe alect2 is likely an underrecognized etiology of chronic kidney disease in certain ethnicities . currently , there is no specific treatment known to be efficacious for the treatment of alect2 . however , it is important to identify this type of amyloidosis so to prevent mistreatment of the disease with the chemotherapeutic agents that are commonly used for treatment of al amyloidosis . most patients have deterioration of renal function after diagnosis with one study showing a mean drop in egfr of 0.5  ml / min/1.73  m per month . another study showed independent predictors of renal survival were serum creatinine at diagnosis ( with a value of 2.0  mg / dl being the best cutoff for predicting esrd ) , percentage global glomerulosclerosis , and presence of diabetes . we present the first large case series detailing the subtypes of renal amyloidosis among egyptians and show that this population has a unique profile of amyloidosis compared with other large renal amyloid case series . unlike case series from the europe and the united states , where al predominates , aa is the most common type of amyloid identified among egyptians . even more surprising is that the newly described lect2 amyloidosis is the second most common and represents nearly one - third of renal amyloid cases in this population . this emerging disease appears to represent an important and likely underrecognized etiology of chronic kidney disease in various ethnic groups around the world .
large case series of renal amyloidosis subtypes have recently been published in the united states and europe showing al amyloidosis to be the predominant subtype in this part of the world . however , the most common subtypes of renal amyloidosis throughout the rest of the world are unknown . 
 we present here the first large case series detailing the subtypes of renal amyloidosis among egyptians . in this population , 
 aa amyloidosis was the most common type of amyloidosis on renal biopsy at 48% . 
 the newly described leukocyte chemotactic factor 2 amyloidosis ( alect2 ) was the second most common type and represented nearly one - third of renal amyloid cases at 31% . 
 al accounted for only 20% of cases . 
 the pathologic findings in alect2 cases were similar to those previously described in other case series . 
 thus alect2 , which was initially thought to affect mainly hispanics in the united states , appears to represent an important and likely underrecognized etiology of chronic kidney disease among egyptians and probably in other ethnic groups around the world .