the online version of this article ( doi:10.1007/s00134 - 012 - 2793 - 8 ) contains supplementary material , which is available to authorized users . fluid challenge techniques have replaced static filling pressures as predictors of preload dependency , being used in clinical practice to evaluate the patient s response to fluids and to avoid pulmonary edema . the generally accepted concept of " fluid responsiveness " is defined at the level of the heart , according to the frank  starling principle . a practical limitation of such an approach is that nonresponders also receive fluids , since the test is evaluated after fluid administration . passive leg raising and functional hemodynamic variables , derived from the assessment of the dynamic interactions between circulation and ventilation , may help to predict " fluid responsiveness " , although being hampered by many practical limitations [ 3 , 4 ] . however , such a macrohemodynamic perspective does not address the fundamental issue of  adequate  organ perfusion . organ perfusion other than by clinical or biochemical signs of organ dysfunction ? and does organ perfusion indeed improve in case of macrohemodynamic  fluid responsiveness  ? new microcirculatory imaging techniques , such as orthogonal polarization spectral ( ops ) imaging   and its technical successor sidestream dark - field ( sdf ) imaging , have allowed direct observation of microcirculation at the bedside . conceptually such techniques enable us to arbitrate tissue perfusion directly . in animal models of hemorrhagic shock blood loss and fluid therapy .   showed that both passive leg raising and volume expansion improved sublingual microcirculatory perfusion during the first 24  h of icu stay in preload - responsive severe sepsis and septic shock patients . others observed that fluid challenge improved microvascular perfusion in the early but not late phase of severe sepsis and suggested this effect to be independent from systemic hemodynamics and type of fluids . the aim of this study is to address the following questions : ( 1 ) if the clinician is confronted with presumed  clinical signs of impaired organ perfusion ,  than what is the actual incidence of abnormal organ perfusion when observed with direct in  vivo microscopy ? ( 2 ) how do patients with abnormal organ perfusion at baseline respond to fluid administration in terms of organ perfusion ( in  vivo microscopy ) and organ function ( clinical signs ) ? ( 3 ) is this response different in comparison with patients with normal organ perfusion at baseline ? ( 4 ) is an improvement in organ perfusion restricted to patients with a rise in stroke volume ( sv ) 10  % after fluid administration ? we anticipated that red blood cell velocity , rather than capillary density , would respond directly to fluid administration ; therefore we chose the microvascular flow index ( mfi ) as the primary variable to quantify organ perfusion . the study was performed between january and march 2011 in a closed - format 22-bed mixed icu in a tertiary teaching hospital . it was designed as a prospective , single - center , observational clinical trial and is registered with clinicaltrials.gov with number nct01369524 . all icu patients of at least 18  years of age with an invasive cardiac output device in  situ were eligible for assessment within the first 6  h of icu admittance . the principal reason for inclusion was the clinical need for fluid therapy , according to one or more predefined signs of impaired organ perfusion : tachycardia > 100  beats / min , after exclusion of pain / discomfort ; impaired skin perfusion ( subjective assessment ) ; oliguria ( < 0.5  ml / kg / h ) ; mean arterial blood pressure ( map ) < 65  mmhg in combination with central / mixed venous oxygen saturation [ s(c)vo2 ] < 65  % ; hyperlactatemia ( > 2.2 a local ethical and scientific committee approved the study protocol , and written informed consent was obtained from the patients or their surrogate decision - makers , consistent with applicable laws . systemic hemodynamic assessment was achieved through continuous invasive monitoring of arterial blood pressure and right heart catheterization with continuous cardiac output measurements ( vigilance ; edwards lifesciences , saint - prex , switzerland ) . alternatively , a designated femoral artery thermodilution catheter was used in combination with a central venous line above the diaphragm ( picco ; pulsion medical systems , munich , germany ) . the general aim of the hemodynamic treatment was cessation of the previously defined signs of impaired organ perfusion . in case these endpoints were not met , the first step was to initiate a fluid challenge . this was performed by infusion of 500  ml crystalloid solution ( nacl 0.9  % ) or balanced colloid solution ( 6  % hydroxyethyl starch 130/0.4 in a sodium acetate - based isotonic solution , volulyte ; fresenius - kabi , bad - homburg , germany ) in 30  min ; the choice of fluid type was made by the attending physician . sidestream dark - field imaging is a stroboscopic light - emitting diode ring - based imaging modality that is incorporated in a handheld device . if a wavelength within the hemoglobin absorption spectrum ( e.g. , 530  nm ) is chosen , red blood cells will appear dark . , a minimum of three steady images of at least 10  s in duration were obtained from the sublingual region by a research investigator other than the attending physician . after gentle removal of saliva by an isotonic - saline - drenched gauze and avoiding pressure artifacts , images were acquired and stored on a digital videotape ( sony video walkman gv - d 1000e ; sony , tokyo , japan ) . subsequently , the images were captured in 510  s representative avi format video clips ( sonydvgate ; sony , tokyo , japan ) . video clips were blindly analyzed offline by an investigator who had no involvement in the data collection . sdf images were obtained from three different locations within the sublingual region , and each image was divided into four equal quadrants . quantification of flow ( no flow : 0 ; intermittent flow : 1 ; sluggish flow : 2 ; continuous flow : 3 ) was scored per quadrant , for each vessel diameter cohort ( small : 1020  m ; medium : 2150  m ; large : 51100  m ) . the mfi was calculated as the sum of each quadrant score divided by the number of quadrants in which the vessel type was visible . the final mfi was averaged over a maximum of 12 quadrants ( three regions , four quadrants per region ) derived from the overall flow impressions of all vessels with a particular range of diameter in a given quadrant . the heterogeneity index was calculated , following the method of trzeciak and colleagues , as the difference between the highest and lowest mfi , divided by the mean mfi of all sublingual sites at a single time point . calculation of total ( small ) vessel density ( tvd ) was performed with the ava  3.0 software package ( microvision medical , amsterdam , the netherlands ) , as described and validated recently , using a cutoff diameter for small vessels of < 20  m . after stabilization of the images using the ava  3.0 software , we defined the perfused ( small ) vessel density ( pvd ) and the proportion of perfused ( small ) vessels ( ppv ) in terms of the number and percentage of crossings with perfused ( small ) vessels per total length of three equidistant horizontal and three equidistant vertical lines . the following data were recorded at baseline : general characteristics , routine laboratory results , ventilator settings , and severity of illness according to acute physiology and chronic health evaluation ( apache ) iv and sequential organ failure assessment ( sofa ) scores , calculated over the first 24  h following icu admission [ 15 , 16 ] . systemic hemodynamic variables , sdf images , arterial lactate concentrations , and blood gases were recorded at baseline and directly after the fluid challenge ( 30  min after baseline ) . at baseline , patient groups were defined according to mfi < 2.6 (  low mfi  ) or mfi 2.6 (  high mfi  ) . the cutoff for mfi was set as 2.6 prior to the start of the study ( clinicaltrials.gov , ntc 00493415 ) , since this reflects the minimum reported lower threshold of the 95  % confidence interval in healthy or nonseptic icu controls [ 12 , 1720 ] . secondary endpoints were the changes in mfi ,  clinical signs of impaired organ perfusion ,  and sv after fluid administration , and the differences in response between groups . alternatively , a study period of 3  months was chosen to obtain a sample size of 3050 patients , in analogy to previous publications [ 7 , 9 , 10 ] . the statistical package for social sciences ( spss 15.1 for windows , chicago , il , usa ) comparison between groups of non - normally distributed microvascular parameters ( mfi , tvd , ppv , pvd ) was performed with a mann  whitney u test . comparison against baseline of systemic and microcirculatory hemodynamic parameters after intervention was performed with a paired t test , or with a wilcoxon signed - rank test in case of normally distributed variables . for dichotomous variables a fisher s exact test was performed . a two - sided p value of < 0.05 was considered statistically significant . the study was performed between january and march 2011 in a closed - format 22-bed mixed icu in a tertiary teaching hospital . it was designed as a prospective , single - center , observational clinical trial and is registered with clinicaltrials.gov with number nct01369524 . all icu patients of at least 18  years of age with an invasive cardiac output device in  situ were eligible for assessment within the first 6  h of icu admittance . the principal reason for inclusion was the clinical need for fluid therapy , according to one or more predefined signs of impaired organ perfusion : tachycardia > 100  beats / min , after exclusion of pain / discomfort ; impaired skin perfusion ( subjective assessment ) ; oliguria ( < 0.5  ml / kg / h ) ; mean arterial blood pressure ( map ) < 65  mmhg in combination with central / mixed venous oxygen saturation [ s(c)vo2 ] < 65  % ; hyperlactatemia ( > 2.2 a local ethical and scientific committee approved the study protocol , and written informed consent was obtained from the patients or their surrogate decision - makers , consistent with applicable laws . systemic hemodynamic assessment was achieved through continuous invasive monitoring of arterial blood pressure and right heart catheterization with continuous cardiac output measurements ( vigilance ; edwards lifesciences , saint - prex , switzerland ) . alternatively , a designated femoral artery thermodilution catheter was used in combination with a central venous line above the diaphragm ( picco ; pulsion medical systems , munich , germany ) . the general aim of the hemodynamic treatment was cessation of the previously defined signs of impaired organ perfusion . in case these endpoints were not met , the first step was to initiate a fluid challenge . this was performed by infusion of 500  ml crystalloid solution ( nacl 0.9  % ) or balanced colloid solution ( 6  % hydroxyethyl starch 130/0.4 in a sodium acetate - based isotonic solution , volulyte ; fresenius - kabi , bad - homburg , germany ) in 30  min ; the choice of fluid type was made by the attending physician . sidestream dark - field imaging is a stroboscopic light - emitting diode ring - based imaging modality that is incorporated in a handheld device . if a wavelength within the hemoglobin absorption spectrum ( e.g. , 530  nm ) is chosen , red blood cells will appear dark . , a minimum of three steady images of at least 10  s in duration were obtained from the sublingual region by a research investigator other than the attending physician . after gentle removal of saliva by an isotonic - saline - drenched gauze and avoiding pressure artifacts , images were acquired and stored on a digital videotape ( sony video walkman gv - d 1000e ; sony , tokyo , japan ) . subsequently , the images were captured in 510  s representative avi format video clips ( sonydvgate ; sony , tokyo , japan ) . video clips were blindly analyzed offline by an investigator who had no involvement in the data collection . sdf images were obtained from three different locations within the sublingual region , and each image was divided into four equal quadrants . quantification of flow ( no flow : 0 ; intermittent flow : 1 ; sluggish flow : 2 ; continuous flow : 3 ) was scored per quadrant , for each vessel diameter cohort ( small : 1020  m ; medium : 2150  m ; large : 51100  m ) . the mfi was calculated as the sum of each quadrant score divided by the number of quadrants in which the vessel type was visible . the final mfi was averaged over a maximum of 12 quadrants ( three regions , four quadrants per region ) derived from the overall flow impressions of all vessels with a particular range of diameter in a given quadrant . the heterogeneity index was calculated , following the method of trzeciak and colleagues , as the difference between the highest and lowest mfi , divided by the mean mfi of all sublingual sites at a single time point . calculation of total ( small ) vessel density ( tvd ) was performed with the ava  3.0 software package ( microvision medical , amsterdam , the netherlands ) , as described and validated recently , using a cutoff diameter for small vessels of < 20  m . after stabilization of the images using the ava  3.0 software , we defined the perfused ( small ) vessel density ( pvd ) and the proportion of perfused ( small ) vessels ( ppv ) in terms of the number and percentage of crossings with perfused ( small ) vessels per total length of three equidistant horizontal and three equidistant vertical lines . this method has been described elsewhere by de  backer et  al . and is in accordance with reports of a round - table conference . the following data were recorded at baseline : general characteristics , routine laboratory results , ventilator settings , and severity of illness according to acute physiology and chronic health evaluation ( apache ) iv and sequential organ failure assessment ( sofa ) scores , calculated over the first 24  h following icu admission [ 15 , 16 ] . systemic hemodynamic variables , sdf images , arterial lactate concentrations , and blood gases were recorded at baseline and directly after the fluid challenge ( 30  min after baseline ) . at baseline , patient groups were defined according to mfi < 2.6 (  low mfi  ) or mfi 2.6 (  high mfi  ) . the cutoff for mfi was set as 2.6 prior to the start of the study ( clinicaltrials.gov , ntc 00493415 ) , since this reflects the minimum reported lower threshold of the 95  % confidence interval in healthy or nonseptic icu controls [ 12 , 1720 ] . secondary endpoints were the changes in mfi ,  clinical signs of impaired organ perfusion ,  and sv after fluid administration , and the differences in response between groups . alternatively , a study period of 3  months was chosen to obtain a sample size of 3050 patients , in analogy to previous publications [ 7 , 9 , 10 ] . the statistical package for social sciences ( spss 15.1 for windows , chicago , il , usa ) was used for statistical analyses . for continuous variables , comparison between groups of non - normally distributed microvascular parameters ( mfi , tvd , ppv , pvd ) was performed with a mann  whitney u test . comparison against baseline of systemic and microcirculatory hemodynamic parameters after intervention was performed with a paired t test , or with a wilcoxon signed - rank test in case of normally distributed variables . for dichotomous variables a fisher s exact test was performed . a two - sided p value of < 0.05 was considered statistically significant . out of 57 patients who were eligible for the study , 50 patients were included ; in seven cases informed consent could not be obtained . sixty - six percent ( 33/50 ) of the patients had mfi < 2.6 at baseline . the median number of  clinical signs of impaired organ perfusion  was 1 ( 12 ) . post  hoc , a receiver operating characteristic ( roc ) curve confirmed a mfi of 2.6 as the optimal cutoff value with respect to the clinical response to fluid administration.table  1baseline characteristicsvariableall ( n  =  50)low mfi ( n  =  33)high mfi ( n  =  17)p - valuemen , n ( % ) 24 ( 48)17 ( 50)7 ( 41)0.49age , years68 ( 6176)68 ( 6276)64 ( 5574)0.32apache iv71 ( 53117)68 ( 53125)72 ( 5195)0.48sofa8 ( 511)8 ( 511)8 ( 410)0.36reason for icu admittance , n ( % )  cardiac surgery22 ( 44)12 ( 37)10 ( 59 )  sepsis12 ( 24)10 ( 30)2 ( 12 )  cpr10 ( 20)6 ( 18)4 ( 23 )  other6 ( 12)5 ( 15)1 ( 6)mean arterial pressure , mmhg66 ( 5975)66 ( 5874)64 ( 5979)0.59heart rate , beats / min91 ( 90106)91 ( 88109)94 ( 90104)0.50central venous pressure , mmhg9 ( 712)9 ( 813)8 ( 611)0.19cardiac index , l / min  m2.3 ( 1.83.3)1.9 ( 1.73.3)2.6 ( 1.93.6)0.22s(c)vo2 , % 69 ( 6273)69 ( 6273)67 ( 6073)0.93dopamine dose , n , g / kg min9 , 5.6 ( 2.510)5 , 8.4 ( 2.812.1)4 , 4.2 ( 2.19.7)0.33norepinephrine dose , n , g / kg  min31 , 0.10 ( 0.060.19)22 , 0.09 ( 0.040.14)9 , 0.19 ( 0.080.29)0.12ventilator , use of , n ( % ) 49 ( 98)33 ( 100)16 ( 94)1.00peep , cmh2o10 ( 1012)10 ( 1013)10 ( 912)0.11lactate , baseline , mmol / l2.2 ( 1.64.2)2.8 ( 1.74.2)1.7 ( 1.44.7)0.43ph7.35 ( 7.287.42)7.35 ( 7.297.41)7.27 ( 7.277.44)0.61hematocrit , % 31 ( 2736)31 ( 2736)31 ( 2941)0.50apache acute physiology and chronic health evaluation , sofa sequential organ failure assessment , s(c)vo2 mixed / central venous oxygen saturation , peep positive end - expiratory pressure , low mfi microvascular flow index < 2.6 , high mfi microvascular flow index 2.6 . all data are presented as median ( iqr ) or as number ( % ) baseline characteristics apache acute physiology and chronic health evaluation , sofa sequential organ failure assessment , s(c)vo2 mixed / central venous oxygen saturation , peep positive end - expiratory pressure , low mfi microvascular flow index < 2.6 , high mfi microvascular flow index 2.6 . all data are presented as median ( iqr ) or as number ( % ) low mfi group ( mfi < 2.6 at baseline , n  =  33 ) : after fluid challenge central venous pressure ( cvp ) , sv index ( svi ) , and s(c)vo2 rose significantly , together with a reduction in heart rate ( table  2 ) . median mfi of small vessels , as a marker of capillary blood flow , increased significantly from 2.3 ( 22.5 ) at baseline to 2.5 ( 2.12.8 ) after fluid challenge ( p  =  0.003 ) ( table  3 ; fig .  1 ) . in conjunction with the rise in mfi the median number of  clinical signs of impaired organ perfusion  reduced from 2 ( 12 ) to 1 ( 02 ) ( p  <  0.001 ) ( fig .  2 ) . the number of patients with a reduction in number of  clinical signs of impaired organ perfusion  after fluid challenge was 15 ( 45  % ) . tvd of small vessels , as a marker of capillary density , did not change significantly from 30 ( 2734 )  mm / mm at baseline to 31 ( 2636 )  mm / mm ( p  =  0.48).table  2systemic hemodynamic variables in response to fluid challengevariableall ( n  =  50)low mfi ( n  =  33)high mfi ( n  =  17)baselinefcbaselinefcbaselinefcmap , mmhg66 ( 5975)68 ( 6179)66 ( 5874)68 ( 5979)64 ( 5979)68 ( 6685)hr , beats / min91 ( 90106)90 ( 86100)91 ( 88109)90 ( 84105)94 ( 90104)90 ( 89 - 100)cvp , mmhg9 ( 712)11 ( 914)9 ( 813)11 ( 914)8 ( 611)10 ( 613)svi , ml / m25 ( 1932)29 ( 2338)22 ( 1929)26 ( 2331)28 ( 2129)31 ( 2534)svi > 10  % , n ( % ) 34 ( 68)23 ( 70)11 ( 65)s(c)vo269 ( 6273)72 ( 6578)69 ( 6273)72 ( 63 - 79)67 ( 6073)71 ( 6679)lactate , mmol / l2.2 ( 1.64.2)2.2 ( 1.43.9)2.8 ( 1.74.2)2.7 ( 1.73.9)1.7 ( 1.44.7)1.5 ( 1.24.2)map mean arterial pressure , hr heart rate , cvp central venous pressure , svi stroke volume index , s(c)vo2 mixed / central venous oxygen saturation , low mfimicrovascular flow index < 2.6 , high mfi microvascular flow index 2.6 , fc fluid challenge . all data presented as median ( iqr)p    0.005 , p  <  0.05 , difference in comparison with baselinetable  3microcirculatory variables in response to fluid challengevariableall ( n  =  50)low mfi ( n  =  33)high mfi ( n  =  17)baselinefcbaselinefcbaselinefcmfi s2.5 ( 2.32.8)2.7 ( 2.42.8)2.3 ( 2.02.5)2.5 ( 2.12.8)2.8 ( 2.82.9)2.8 ( 2.73)mfi l3 ( 2.93)3 ( 33)3 ( 2.93)3 ( 33)3 ( 2.93)3 ( 33)het mfi0.3 ( 0.10.3)0.2 ( 0.10.3)0.3 ( 0.20.5)0.3 ( 0.10.4)0.2 ( 0.10.2)0.2 ( 0.10.3)ppv s ( % ) 90 ( 8693)91 ( 8995)88 ( 8491)91 ( 8494)94 ( 9295)94 ( 9196)tvd  s , mm / mm29 ( 2734)31 ( 2634)30 ( 2734)31 ( 2636)27 ( 2532)28 ( 2332)pvd  s , 1/mm14 ( 1217)15 ( 1318)14 ( 1217)16 ( 1318)15 ( 1416)15 ( 1217)mfi s microvascular flow index small vessels ( < 20  m ) , mfi l microvascular flow index large vessels ( 20  m ) , het mfi coefficient of mfi s variation , ppv s percentage of perfused small vessels , tvd s total vessel density small vessels , pvd s perfused vessel density small vessels , low mfi microvascular flow index < 2.6 , high mfi microvascular flow index 2.6 , fc fluid challenge . 1boxplots of the microvascular flow index ( mfi ) in response to fluid administration in patients with mfi < 2.6 ( low mfi ) and 2.6 ( high mfi ) at baseline . * 2change in number of  clinical signs of impaired organ perfusion  for individual patients in response to fluid administration . closed lines low mfi group ( mfi < 2.6 at baseline ) , dotted lines high mfi group ( mfi 2.6 at baseline ) . * p  <  0.001 systemic hemodynamic variables in response to fluid challenge map mean arterial pressure , hr heart rate , cvp central venous pressure , svi stroke volume index , s(c)vo2 mixed / central venous oxygen saturation , low mfimicrovascular flow index < 2.6 , high mfi microvascular flow index 2.6 , fc fluid challenge . all data presented as median ( iqr ) p    0.005 , p  <  0.05 , difference in comparison with baseline microcirculatory variables in response to fluid challenge mfi s microvascular flow index small vessels ( < 20  m ) , mfi l microvascular flow index large vessels ( 20  m ) , het mfi coefficient of mfi s variation , ppv s percentage of perfused small vessels , tvd s total vessel density small vessels , pvd s perfused vessel density small vessels , low mfi microvascular flow index < 2.6 , high mfi microvascular flow index 2.6 , fc fluid challenge . all data presented as median ( iqr ) . p    0.005 in comparison with baseline boxplots of the microvascular flow index ( mfi ) in response to fluid administration in patients with mfi < 2.6 ( low mfi ) and 2.6 ( high mfi ) at baseline . * p  <  0.005 change in number of  clinical signs of impaired organ perfusion  for individual patients in response to fluid administration . closed lines low mfi group ( mfi < 2.6 at baseline ) , dotted lines high mfi group ( mfi 2.6 at baseline ) . * p  <  0.001 high mfi group ( mfi 2.6 at baseline , n  =  17 ) : after fluid challenge svi rose significantly , together with a reduction in heart rate ( table  2 ) . however , median mfi of small vessels remained unaltered : 2.8 ( 2.82.9 ) at baseline versus 2.8 ( 2.73 ) ( p  =  0.45 ) ( table  3 ; fig .  1 ) . also , the number of  clinical signs of impaired organ perfusion  did not change significantly , from 1 ( 12 ) at baseline versus 1 ( 12 ) after fluid challenge ( p  <  0.32 ) ( fig .  2 ) . the number of patient with a reduction in number of  clinical signs of impaired organ perfusion  after fluid challenge was 3 ( 18  % ) . in comparison with the low mfi group this reduction was significantly lower ( 45 vs. 18  % , p  =  0.05 ) . tvd of small vessels did not change significantly : from 27 ( 2532 )  mm / mm at baseline , to 28 ( 2332 )  mm / mm ( p  =  0.72 ) . responders versus nonresponders ( svi 10  % vs. svi < 10  % ): in 68  % of all patients svi increased 10  % after fluid challenge ( table  2 ) . there was no difference in the number of responders between the low mfi and high mfi groups : 23/33 ( 70  % ) versus 11/17 ( 65  % ) ( p  =  0.78 ) . in the responders ( n  =  34 ) , mfi of small vessels rose significantly from 2.5 ( 2.32.8 ) at baseline to 2.7 ( 2.42.8 ) after fluid administration ( p  =  0.02 ) . the median number of  clinical signs of impaired organ perfusion  reduced from 1.5 ( 12 ) to 1 ( 12 ) ( p  =  0.01 ) . in the nonresponders ( n  =  16 ) , mfi of small vessels also rose from 2.5 ( 2.22.9 ) to 2.7 ( 2.42.9 ) , but this was insignificant ( p  =  0.09 ) . the median number of  clinical signs of impaired organ perfusion  reduced from 2 ( 13 ) to 1.5 ( 12 ) ( p  =  0.01 ) . in the present study , sublingual microvascular flow alterations , defined as mfi < 2.6 , were present in two - thirds of mixed icu patients with presumed  clinical signs of impaired organ perfusion . this indicates a rather low specificity of widely used clinical criteria that intensive care doctors assume to be indicators of impaired organ perfusion . in the low mfi group , fluid administration was accompanied by significant attenuation of impaired microvascular blood flow and reduction of  clinical signs of impaired organ perfusion . however , in the high mfi group , fluid administration was associated with neither a change in microvascular blood flow nor mitigation of these clinical signs , suggesting alternative causes for such symptoms in these patients . assessment of microcirculatory flow abnormalities , prior to fluid therapy , may therefore be helpful to increase the pre - test likelihood of such intervention . the data from this study underline the importance of selecting patients eligible for fluid therapy and monitoring the effects . since none of the macrohemodynamic variables at baseline discriminated for mfi < 2.6 , it seems relevant to assess organ perfusion at the level of the microcirculation . the fact that scoring of mfi has been demonstrated to be feasible in real time at the bedside adds to its clinical applicability for selecting patients and monitoring therapeutic interventions . as shown in our data , it is of note to use variables of flow ( mfi or ppv ) rather than variables that represent capillary distance ( pvd , tvd ) . apart from our newly suggested approach to select patients prior to fluid therapy , our data seem to add to the existing literature in other perspectives as well . previous publications linked ( the persistence of ) rather gross microvascular alterations to organ dysfunction , severity of illness , and prognosis [ 12 , 17 , 2224 ] . in these studies , with relatively small sample sizes , strict selection of specific subgroups of patients , with profound abnormalities at baseline , are helpful to detect potential mechanisms . however , such studies do not mimic clinical reality to the full extent . clinical decision - making in the icu is often characterized by uncertainties regarding whether to intervene in relatively mild abnormalities in a heterogeneous population . in our study , median mfi was  only  2.5 and median ppv 90  % , with a lowest 25th percentile at 2.3 and 86  % , respectively . these data add to the understanding that even relatively mild microvascular flow alterations are associated with clinical signs of impaired organ perfusion , and may be mitigated by therapy , before transition to prolonged organ dysfunction [ 20 , 25 ] . furthermore , our data seem to expand the existing knowledge of microvascular abnormalities and response to fluid therapy , from predominantly sepsis patients [ 9 , 10 ] to a more heterogeneous icu population . ( i.e. , svi 10  % in response to a fluid challenge ) and the mitigation of microcirculatory abnormalities seems to be rather complex . at baseline , the static dichotomous variable responder / nonresponder discriminated neither between mfi < 2.6 or 2.6 nor between the presence or absence of  clinical signs of impaired organ perfusion . this highlights the issue that a significant increase in sv after fluid administration is not equal to the need for fluid therapy . in daily practice not only is  fluid responsiveness  difficult to predict , but means (  responsiveness  ) and objective ( improvement of organ perfusion ) are also often mistaken . moreover , the absolute increase in mfi was equal in patients with and without an increase in svi 10  % . our observations suggest that , within the 30-min time window of the fluid challenge , microcirculatory flow alterations may improve , independent from the change in sv . this seems to be in contradiction to the idea that a systemic enhancement of flow ( e.g. , cardiac output ) is a prerequisite for an augmentation of organ perfusion at the level of the microcirculation . these data seem to be in line with the existing literature . in preload - dependent septic patients , with marked microvascular alterations at baseline , the effect of fluid therapy was reflected both at the level of the heart and in the microcirculation . others observed a time - dependent relation between fluid administration and microvascular response in septic patients , irrespective of ( a change in ) blood pressure or cardiac output . however , the fact that , in this study , microvascular variables were considerable higher in the late phase of sepsis than in the early phase also underpins the importance of establishing the magnitude of potentially correctable abnormalities prior to intervention . although the observation period was short ( 30  min ) , spontaneous improvement in microvascular blood flow can not be ruled out . however , as of now , denial of fluids is considered unethical under these circumstances . secondly , the absence of effect on clinical signs of organ perfusion may have been hampered by the chosen timeframe and amount / type of fluid administered . clinical signs may have needed longer time to respond to the intervention , and in individual patients more fluids may have been necessary to detect effects . the fact that both crystalloid and colloid solutions have been used is of potential concern . some authors observed marked differences in microvascular response after 24  h in sepsis with either isotonic saline or he s 6  % 130/0.4 , whereas others could not observe such difference between ringer s lactate and 4  % albumin , or colloids and hypertonic saline [ 7 , 28 ] . in a post hoc analysis we were unable to demonstrate significant differences in sv and mfi in response to different types of fluids ( electronic supplementary material ) . thirdly , the definitions of impaired organ perfusion were chosen in line with a local protocol , rather than a more standardized sofa score . although small but significant changes in sofa score have been described to be associated with microvascular alterations during protocol - directed resuscitation , we considered large parts of the sofa score potentially insensitive to detect changes in organ perfusion . it is conceivable that other organs may express different microvascular abnormalities at baseline [ 18 , 29 ] , or in response to fluid administration . in the present study microvascular flow abnormalities , defined as mfi < 2.6 , were present in two - thirds of hemodynamically unstable icu patients with in response to fluid administration , a significant increase in microvascular blood flow and attenuation of  clinical signs of impaired organ perfusion  were restricted to patients with mfi < 2.6 at baseline . these data add to the understanding that noninvasive assessment of microvascular perfusion may help to identify patients with potential need for fluid therapy , and to evaluate its effect . supplementary figure  1 flowchart of patient inclusion according to baseline microvascular flow index ( mfi ) and type of fluid ( tiff 58  kb ) supplementary figure  2 boxplots of the microvascular flow index ( mfi ) in response to fluid administration in different types of fluids . * p  <  0.05 ( tiff 94  kb ) supplementary figure  3 boxplots of the stroke volume index in response to fluid administration in different types of fluids . * p  <  0.05 ( tiff 92  kb )
purposethe aim of this study is to assess the incidence of sublingual microcirculatory flow alterations , according to a predefined arbitrary cutoff value , in patients with  clinical signs of impaired organ perfusion  . 
 secondary endpoints were the changes in microvascular flow index ( mfi ) ,  clinical signs of impaired organ perfusion  , and stroke volume ( sv ) after fluid administration , and the differences between groups.methodsprospective , single - center , observational study in a 22-bed mixed intensive care unit ( icu ) . 
 patients 18  years with invasive hemodynamic monitoring and  clinical signs of impaired organ perfusion  as the principal reason for fluid administration were included . before and after fluid challenge , systemic hemodynamics and direct in  vivo observation of the sublingual microcirculation with sidestream dark - field imaging were obtained . 
 microvascular flow index ( mfi ) < 
 2.6 was predefined as abnormal.resultsn  =  50 . at baseline , mfi 
 < 2.6 was present in 66  % of the patients . in these patients , 
 mfi increased from 2.3 ( 22.5 ) at baseline to 2.5 ( 2.12.8 ) after fluid challenge ( p  =  0.003 ) . 
 this was accompanied by a reduction in the number of  clinical signs of impaired organ perfusion  from 2 ( 12 ) to 1 ( 02 ) ( p  <  0.001 ) . however , in patients with mfi > 2.6 at baseline , mfi and clinical signs changed insignificantly [ 2.8 ( 2.82.9 ) versus 2.8 ( 2.73 ) , p  =  0.45 , respectively , 1 ( 12 ) versus 1 ( 12 ) , p  <  0.32 ] . 
 these changes were not restricted to patients with a rise in sv 10  % .conclusionsthese data add to the understanding that noninvasive assessment of microvascular blood flow may help to identify patients eligible for fluid therapy , and to evaluate its effect.electronic supplementary materialthe online version of this article ( doi:10.1007/s00134 - 012 - 2793 - 8 ) contains supplementary material , which is available to authorized users .