protein  protein interactions ( ppis ) play a vital role in initiating infection in a number of pathogens . for example , hiv uses host surface proteins to gain entrance to the host cell . hiv protein env attaches to the host human glycoprotein cd4 and subsequently to host chemokine receptors ccr5 and cxcr4 . these binding events cause conformational changes to viral proteins that allow the membrane of the virus to fuse to the host cell membrane and enable the virus to enter the host cell . knowing which ppis allow a pathogen to infect its host provides critical insights into methods of pathogenesis and potential targets for therapeutics . to this end we have created the pathogen interaction gateway ( pig ) , an integrated platform of experimentally verified and manually curated host pathogen ppis ( hp  ppis ) and associated computational tools . currently there are a number of public databases ( 14 ) and other resources ( e.g. http://www.proteomicsresource.org ) that store data for experimentally verified and manually curated host  pathogen ppis . pig is designed to integrate data from these various public resources and primary literature into a single data warehouse . pathogen ppis ; data for other host  pathogen systems will be included as they become available . our goals for pig are : ( i ) to create a centralized location for experimentally verified and manually curated hp  ppis that is integrated with other public resources ; ( ii ) to provide an easy - to - use web interface for accessing and using data that would otherwise require the navigation of several websites ; ( iii ) to provide a platform upon which various tools can be developed for identifying potential targets for therapeutics and ( iv ) to set the stage for developing methods for predicting host  pathogen ppis . we designed a postgresql relation database to store the data in pig . from each interaction database , we gather important information for each hp  ppi including protein ids , organism information , literature references and the experimental method used to identify the interaction . next , we map all protein ids to uniprot ( 5 ) entry ids to allow for easy integration of other external resources and genomic information . we ignore any interaction in which a protein does not have a mapping to the uniprot system . we focus on hp  ppis corresponding to host  pathogen systems of interest ( currently only human pathogens ) . we then create a non - redundant set of hp  ppis and insert them into pig . in addition to hp  ppis we also gather and integrate intra - species ppis from public resources ( 1,3,4,69 ) . we obtain protein sequence information from uniprot ( 5 ) , functional annotations for protein entries from the gene ontology ( 10 ) and functional domain data from interproscan ( 11 ) . users can download the data stored in pig as either a postgresql dump or as tab - delimited files . pathogen ppi data currently stored in piggroupnumber of ppisnumber of strainsnumber of referenceshiv9095491035yersinia411135bacillus3077311francisella138311hepatitis12441695influenza28745papillomavirus22912177epstein bar virus206236adenovirus82961herpesvirus632056sarcoma virus51657clostridium4545t - lymphotrophic virus25212escherichia coli2227chlamydia2023neisseria1613streptococcus1457vaccinia virus1348staphylococcus12317pseudomonas1114measles virus1035polyomavirus8311leukemia virus711shigella617plasmodium622anemia virus448hantaan virus411sars415listeria413salmonella314dengue virus333seoul virus311echovirus322helicobacter325rotavirus333foamy virus211siv221dictyostelium212puumala virus211orf virus221aeromonas211stomatitis virus211mycoplasma211bothrops211campylobacter211vipera111sendal virus111pneumocystis111corynephage111nucleopolyhedrovirus111candida111rabies virus111toxoplasma111poliovirus111nipah virus111klebsiella111enterobacteria111mokola virus111west nile virus111tula virus111ebolavirus111total20 1132061322for each host  pathogen system , we list the number of known ppis , the number of strains and the number of literature references . pathogen ppi data currently stored in pig for each host  pathogen system , we list the number of known ppis , the number of strains and the number of literature references . pig contains a number of tools for identifying data of interest . first , pig has two search tools : a simple text search ( figure 1a ) and a blastp ( 12 ) interface ( figure 1b ) . the text interface allows users to search the database for key terms of interest ( e.g. protein identifier or protein name ) . valid results return links to protein - specific pages that contain information about the protein itself , such as functional annotations and domains , along with a list of interactions in which the protein participates ( figure 1c ) . protein attributes such as i d , functional annotations and domains are hyperlinked to their respective external websites to allow easy navigation among different resources ( figure 1d ) . the blastp interface allows users to search entries in pig that have sequence similarity to a protein of interest . results are displayed in a standard fashion and each significant hit links back to a protein - specific page in pig . users can search data within pig using either ( a ) a simple text search or ( b ) a blast interface . the individual protein pages contain information functional annotations , domains , and known inter - species and intra - species ppis . ( d ) each piece of information contains a direct link to the corresponding external database . users can select a host  pathogen system of interest and ( f ) view the corresponding network . users can use the genomic information in pig to view subsets of the networks using ( g ) domain , ( h ) annotation and ( i ) experimental method data . ( j ) users can follow links from the visualization page to corresponding external databases . users can search data within pig using either ( a ) a simple text search or ( b ) a blast interface . the individual protein pages contain information functional annotations , domains , and known inter - species and intra - species ppis . ( d ) each piece of information contains a direct link to the corresponding external database . users can select a host  pathogen system of interest and ( f ) view the corresponding network . users can use the genomic information in pig to view subsets of the networks using ( g ) domain , ( h ) annotation and ( i ) experimental method data . ( j ) users can follow links from the visualization page to corresponding external databases . in addition to search tools , pig also provides a platform for visualizing hp  ppi networks . the user first selects a host system and a group of pathogens ( figure 1e ) . each group entry expands to list - specific strains , thus allowing a user to compare closely related strains of a particular pathogen or pathogens from two different groups . networks can be visualized directly within a web browser using a custom applet ( java required ) , or downloaded as graphml files for offline use ( figure 1f ) . our custom applet provides full interactivity to the user , including zooming and panning , click - and - drag , and rich contextual information . the visualization of two hp  ppi networks allows users to identify human proteins that interact with both pathogens . these conserved interactors can provide critical insights into general strategies employed by pathogens during pathogenesis . the visualization tool also allows users to quickly identify functional annotations and domains for a selected protein in the network . for each of the protein 's interactors , the supporting literature references are also displayed along with the technology used to identify the interaction . these features can be used to visualize subsets of the hp  ppi network ( e.g. those interactions identified using a yeast two - hybrid approach ) ( figure 1g each of these features also links to the corresponding external website ( figure 1j ) . finally , users can restrict a network using go - slim terms ( 10 ) to focus on specific functional sub - networks . pig is an integrated resource that acts a centralized location for public information on known hp each protein entry in pig is hyperlinked to its corresponding entry in the uniprot database ( 5 ) , functional annotations to the gene ontology ( 10 ) , functional domains to interproscan ( 11 ) and interactions to pubmed entries . pig includes a number of tools for accessing available data including a simple text search , a blast interface and a tool for visualizing and comparing hp  ppi networks . future improvements of pig will include the integration of additional data sources such as known virulence factors and toxins ( 9 ) , tools for identification of therapeutic targets and computational methods to allow users to predict ppis between any host and pathogen system of their choice . we believe that pig will be a valuable resource for researchers of host  pathogen systems and possibly aid in the identification of potential targets for therapeutics . national institute of allergy and infectious diseases grant hhsn26620040035c . funding for open access charge : nih grant .
protein  protein interactions ( ppis ) play a vital role in initiating infection in a number of pathogens . identifying which interactions allow a pathogen to infect its host can help us to understand methods of pathogenesis and provide potential targets for therapeutics . 
 public resources for studying host  pathogen systems , in particular ppis , are scarce . to facilitate the study of host  pathogen ppis , 
 we have collected and integrated host 
 pathogen ppi ( hp  ppi ) data from a number of public resources to create the pathogen interaction gateway ( pig ) . 
 pig provides a text based search and a blast interface for searching the hp  ppi data . 
 each entry in pig includes information such as the functional annotations and the domains present in the interacting proteins . 
 pig provides links to external databases to allow for easy navigation among the various websites . 
 additionally , pig includes a tool for visualizing a single hp  ppi network or two hp  ppi networks . 
 pig can be accessed at http://pig.vbi.vt.edu .