several animal models have been used as an experimental basis to determine molecular and cellular mechanisms underlying and controlling an undisturbed healing process . poffenbarger and haberal reported that surgical or burn trauma produced an increase in serum nonresponsible insulinlike activity ( nsila ) in an experimental model , and they showed its role in the tissue repair process . some of the findings show that low - frequency pulsed electromagnetic fields accelerate skin wound healing and prevent necrosis . however , other studies have demonstrated that pulsed electromagnetic fields did not have a significant beneficial effect on wound healing . use of static magnetic fields on different kinds of tissue defects can produce satisfying therapeutic effects.[58 ] research has shown that low - power static magnetic fields increased the rate of wound healing , but no study to date has examined high - static magnetic fields effect on primary wound healing . in this study , we examined the effects of an externally applied electromagnetic field , a static magnetic field generated by a permanent nefeb magnet , bidirectionally to skin wound healing and strength in rats . animals were acclimatized to laboratory conditions for 1 week before the experiments , and were fed standard rat chow , and given free access to water throughout the study . eighty rats were divided into 2 groups : those containing magnetic bar and those containing a nonmagnetic bar . each group was further divided into 2 subgroups , depending on the route the bars took ( parallel or perpendicular ) . each subgroup was then further divided into 2 more subgroups according to the postoperative day on which it was examined ( day 7 or day 14 ) . forty of these 80 animals ( m group ) had nefeb magnets ( the product has been described in detail previously ) placed near the incisions ( 0.5 cm away ) , either parallel ( pa ; the north seeking pole was positioned cranially ) or perpendicular ( pr ; the north seeking pole was positioned close to the midline ) to the incision . ( the magnetic field strength at the site was 390 to 420 gauss . ) to prevent inadvertent displacement , the bars were fixed to the skin by placing a 2 - 0 silk suture in a groove that the manufacturer had made at each pole of the bar . the other 40 animals ( the sham [ s ] group ) had nonmagnetized nefeb bars going in the same directions , dimensions , and weights implanted . half of the animals in each group were killed by anesthetic overdose and assessed for healing parameters on postoperative day 7 ( groups mpa7 , mpr7 , pa7 , and spr7 ) , and the other half was killed on postoperative day 14 ( groups mpa14 , mpr14 , spa14 , and spr14 ) . animals were anesthetized with a combination of intraperitoneally administered 5 mg / kg xylazine ( rompun , bayer , istanbul , turkey ) and 30 mg / kg ketamine hydrochloride ( ketalar , parke - davis , istanbul , turkey ) . the incision was made 3 cm away from the dorsal midline and parallel to the midline . the wounds were then sutured with continuous 4 - 0 nylon ( ethicon , inc , somerville , nj , usa ) . for postoperative analgesia , beginning on the day after the surgery , 0.02 mg / kg fentanyl citrate ( fentanyl ; abbott , chicago , il , usa ) after each rat in the magnetized and nonmagnetized groups was killed , 4 types of evaluations were done . macroscopic examination included wound dehiscence , suture reaction , scar formation , infection , and abscess formation . ten animals in each group ( mpa7 , mpr7 , spa7 , and spr7 ) were killed on day 7 , and the other groups ( mpa14 , mpr14 , spa14 , and spr14 ) were killed on day 14 after wounding . the dorsal pelt containing the healing scar was removed and cut at a right angle to the long axis of the wound into four 10-mm wide strips . the strips were placed in a buffered ringer 's solution ( ph 7.4 ) and used within 30 minutes of recovering the pelt to assess breaking strength . biomechanical tests were performed using an instron model 8874 ( buckinghamshire , uk ) universal material testing system . grip length was selected as 10 mm on both sides and fine - grade sandpapers were placed inside the grips to prevent slipping . tests were performed at a constant speed of 1 mm / minute until breakage at the healing scar was observed . force was measured with a 250-n load - cell attached to the testing frame , and test results were stored on a personal computer for further analysis . incisional skin biopsies of wounded mice were formalin - fixed , paraffin - embedded , routinely processed , and stained with hematoxylin and eosin . hematoxylin and eosin - stained sections were analyzed for the following histologic features ( as defined by muehlberger and associates ) : epidermal alterations ( including acanthosis and surface integrity ) , and dermal alterations ( including granulation tissue and inflammatory response ) . epidermal thickness was given by the number of layers , and surface integrity was decided whether focal erosions were present or absent . in addition , dermal alterations were scored semiquantitatively from 0 to 4 ( 0 , absent ; 1 , minimal ; 2 , mild ; 3 , moderate ; 4 , severe ) . pairwise differences were compared using the mann - whitney u or t test as appropriate . eighty rats were divided into 2 groups : those containing magnetic bar and those containing a nonmagnetic bar . each group was further divided into 2 subgroups , depending on the route the bars took ( parallel or perpendicular ) . each subgroup was then further divided into 2 more subgroups according to the postoperative day on which it was examined ( day 7 or day 14 ) . forty of these 80 animals ( m group ) had nefeb magnets ( the product has been described in detail previously ) placed near the incisions ( 0.5 cm away ) , either parallel ( pa ; the north seeking pole was positioned cranially ) or perpendicular ( pr ; the north seeking pole was positioned close to the midline ) to the incision . ( the magnetic field strength at the site was 390 to 420 gauss . ) to prevent inadvertent displacement , the bars were fixed to the skin by placing a 2 - 0 silk suture in a groove that the manufacturer had made at each pole of the bar . the other 40 animals ( the sham [ s ] group ) had nonmagnetized nefeb bars going in the same directions , dimensions , and weights implanted . half of the animals in each group were killed by anesthetic overdose and assessed for healing parameters on postoperative day 7 ( groups mpa7 , mpr7 , pa7 , and spr7 ) , and the other half was killed on postoperative day 14 ( groups mpa14 , mpr14 , spa14 , and spr14 ) . animals were anesthetized with a combination of intraperitoneally administered 5 mg / kg xylazine ( rompun , bayer , istanbul , turkey ) and 30 mg / kg ketamine hydrochloride ( ketalar , parke - davis , istanbul , turkey ) . the incision was made 3 cm away from the dorsal midline and parallel to the midline . the wounds were then sutured with continuous 4 - 0 nylon ( ethicon , inc , somerville , nj , usa ) . for postoperative analgesia , beginning on the day after the surgery , 0.02 mg / kg fentanyl citrate ( fentanyl ; abbott , chicago , il , usa ) was administered subcutaneously , 2 times daily , for 3 days . after each rat in the magnetized and nonmagnetized groups was killed , 4 types of evaluations were done . macroscopic examination included wound dehiscence , suture reaction , scar formation , infection , and abscess formation . ten animals in each group ( mpa7 , mpr7 , spa7 , and spr7 ) were killed on day 7 , and the other groups ( mpa14 , mpr14 , spa14 , and spr14 ) were killed on day 14 after wounding . the dorsal pelt containing the healing scar was removed and cut at a right angle to the long axis of the wound into four 10-mm wide strips . the strips were placed in a buffered ringer 's solution ( ph 7.4 ) and used within 30 minutes of recovering the pelt to assess breaking strength . biomechanical tests were performed using an instron model 8874 ( buckinghamshire , uk ) universal material testing system . grip length was selected as 10 mm on both sides and fine - grade sandpapers were placed inside the grips to prevent slipping . tests were performed at a constant speed of 1 mm / minute until breakage at the healing scar was observed . force was measured with a 250-n load - cell attached to the testing frame , and test results were stored on a personal computer for further analysis . incisional skin biopsies of wounded mice were formalin - fixed , paraffin - embedded , routinely processed , and stained with hematoxylin and eosin . hematoxylin and eosin - stained sections were analyzed for the following histologic features ( as defined by muehlberger and associates ) : epidermal alterations ( including acanthosis and surface integrity ) , and dermal alterations ( including granulation tissue and inflammatory response ) . epidermal thickness was given by the number of layers , and surface integrity was decided whether focal erosions were present or absent . in addition , dermal alterations were scored semiquantitatively from 0 to 4 ( 0 , absent ; 1 , minimal ; 2 , mild ; 3 , moderate ; 4 , severe ) . pairwise differences were compared using the mann - whitney u or t test as appropriate . they were killed at a designated time , and there were no deaths during the experiment . there was no wound dehiscence , superficial or subcutaneous infections , or hypertrophic scar formation determined in the groups at gross examination . the meanstandard deviation tensile strength values for groups mpa7 , mpr7 , spa7 , and spr7 were 1.50.3 , 2.81.5 , 1.40.5 , and 1.20.6 ( p=.001 ) . after a pairwise comparison using the mann - whitney u test , the mean skin wound tensile strength was found to be significantly higher in the mpr 7 group than in the other groups ( mpa7 vs spr7 ; p=.04 ; mpa7 vs mpr7 ; p=.02 ; mpa7 vsspa7 ; p=.1 ; spr7 vs mpr7 ; p=.01 ; spr7 vs spa7 ; p=.5 ; and mpr7 vs spa7 ; p=.007 ) [ figure 1 ] . on the 14 day , tensile strength values for groups mpa14 , mpr14 , spa14 , and spr14 were 3.10.8 , 74.4 , 2.20.5 , and 2.21.3 ( p>.001 ; figure 1 ) . the mpr14 group had significantly higher tensile strength than the other groups ( mpa14 vs spr14 ; p=.1 ; mpa14 vs mpr14 ; p=.001 ; mpa14 vsspa14 ; p=.004 ; spr14 vs mpr14 ; p=.006 ; spr14 vs spa14 ; p=.9 ; and mpr14 vs spa14 ; p=.003 ) . specifically , the tensile strength was statistically higher in group mpa14 than it was in groups spr14 and spa14 [ figure 1 ] . breaking strength measurements of the groups in the first and second week there was no wound dehiscence , hypertrophic scar , or abscess formation and superficial infection when gross healing was evaluated . epidermal thickness was nearly same as that seen in both magnetically treated and untreated wounds . the mpr7 and mpr14 groups had higher scores for epidermal thickness , but this difference was not statistically significant . breaking strength ( n ) values in groups granulation tissue scores are insignificantly higher in the mpr14 group when compared with the other groups . the mpr7 group had a lower inflammatory reaction than did the other groups , but were statistically insignificant . in the second week , all groups had the same values for inflammatory reaction we used a bidirectional static magnetic field to the dermal incision , and found that exposure of a static magnetic field placed perpendicular to the incision increases the strength of cutaneous wounds that are closed primarily . two methods have been intensely investigated , and both positive and negative results have been reported . pulsed electromagnetic field studies were differentiated according to intensity , vector , and exposure times of the magnetic field . evidence has shown us that pulsed electromagnetic fields affect tissues as they undergo metabolic changes at the cellular level , especially during the healing process . basically , there are general problems when applying pulsed electromagnetic fields on dermal and other tissues . while experimentally used pulsed electromagnetic fields affect the whole body , it is difficult to investigate the net local effect of the magnetic field . animals placed in cages have stable positions according to the vector of a pulsed electromagnetic field . to lessen the stress of test animals , application of static magnetic fields is simple , and it achieves a permanent magnetic effect and a permanent vectorial effect . static magnetic fields are not related to electric energy , as no heat and electricity harms the tissues . at the same time , the magnetic force applied locally , but not to the whole body or surrounding tissues , have minimal exposure . medical applications of a magnetic field are generally reported as successful in musculoskeletal disease , but there is a lack of explanation about a specific molecular mechanism of magnetic field effect . rosen and associates reported the magnetic field effect depends on diamagnetic anisotropic properties of membrane phospholipids in moderate - intensity magnetic field . according to miyakoshi the magnetic field of the earth has a strength of 0.5 g , which is equivalent to about 510 t. in this study , we used the magnetic bars that have permanent 4 mt field at the poles and 0.4 mt at the incision site . murayama and associates first reported exposure to 0.35 t placed sickled erythrocytes perpendicular to a magnetic field . there are varying results about the orientation of cells depending on the cell type , intensity , and application time of a static magnetic field . thus , in this study , we placed the magnets either parallel or perpendicular ( according to the incision ) . hoping that at least 1 of the vectorial effects of the static magnetic fields could enhance wound healing by aligning the cells perpendicular to incision . in vivo studies high static magnetic field intensity decreased resting finger skin microcirculation in humans , moderate intensity influenced arteriolar diameters , and reduces edema formation in rats . more detailed investigations about the effect of electrical fields and pulsed electromagnetic fields have been done . when an electrical field is applied to wound edges locally we do not know if there is magnetotaxis or magneto - rotational instability related to the magnetic field . in this study , we did not investigate the magneto - rotational instability or histologically cellular alignment in the wound . static magnetic fields have some effects on moving biological features , like blood or active cellular organelles . magnetic force affects in a perpendicular angle to change the momentum of something by processing the direction to make a magnetic moment . there have been few studies on the effects of static magnetic fields at the cellular level . buemi and associates showed that a 0.5-mt static magnetic field exposure increases necrotic morphology , but this effect varies at the cell type . cell cycle analysis did not differ when 1.5- to 7-t was exposed for 24 hours . static 0.2-t magnetic field exposed to gingival fibroblasts for 6 or 8 months did not show increased proliferation . five - hour exposure of 6-mt static magnetic field had no apoptotic or necrotic effect on hl-60 cells . in this study , we used the magnetic bars that had permanent 4 mt fields at the poles and 0.4 mt at the incision site . in rats with magnets , the epidermal thickness was insignificantly high in the first week , but not in the second week . we observed no statistical difference in granulation tissue and inflammation in the groups in both weeks . dermal strength is an important measure because it best describes the mechanical property of skin . we found that perpendicular static magnetic treatment of the wound increased the wound breaking strength when compared to the other groups in the first or second week ; especially , when the parallel magnetic effect did not affect wound - breaking strength , as it did in the perpendicular group . the reason for this may be that the classic direction of cell migration in the wound is between the wounded edges or the reparative cells arranged perpendicularly to the static magnetic field . maybe the direction of the static effect is the same direction of cell migration when the magnet is perpendicular to the wound , so rotation or alignment of the cells can be affected from the geomagnetic field . in conclusion , the static magnetic field we tested in this study increased the mechanical strength of an experimental rat in a dermal wound model .
context : an animal model.aim:we sought to evaluate the effect of static magnetic fields on cutaneous wound healing.materials and methods : male wistar rats were used . 
 wounds were created on the backs of all rats . 
 forty of these animals ( m group ) had nefeb magnets placed in contact with the incisions , either parallel ( pa ) and perpendicular ( pr ) to the incision . 
 the other 40 animals ( sham [ s ] group ) had nonmagnetized nefeb bars placed in the same directions as the implanted animals . 
 half of the animals in each group were killed and assessed for healing on postoperative day 7 and the other half on postoperative day 14 . 
 the following assessments were done : gross healing , mechanical strength , and histopathology.statistical analysis used : intergroup differences were compared by using the mann - whitney u or t test . values for p less than 0.05 were accepted as significant.results and conclusions : there were no differences between the magnetic and sham animals with respect to gross healing parameters . 
 the mechanical strength was different between groups . 
 on postoperative day 14 , the mpr14 had significantly higher scores than the other groups . 
 when static , high - power , magnetic fields are placed perpendicular to the wound , increased wound healing occurs in the skin of the experimental model .