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abstractthe crisprcas9 gene editing system requires cas9 endonuclease and guide rnas ( either the natural dual rna consisting of crrna and tracrrna or a chimeric single guide rna ) that direct sitespecific doublestranded dna cleavage . 
 this communication describes a click ligation approach that uses alkyne 
 azide cycloaddition to generate a triazolelinked single guide rna ( sgrna ) . 
 the conjugated sgrna shows efficient and comparable genome editing activity to natural dual rna and unmodified sgrna constructs .