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Beyond the quilt!: 09/26/13
Where the brass bands play!
Suffering from a big dose of cabin fever I was delighted when my hubby asked if I would like to join him on a walk along the promenade. I usually turn any invitation down as I am so busy in my sewing room but after nearly three weeks of non stop sewing I needed to GET OUT!
I really do love being home ...and although I don't often do any beachy things I enjoy pausing at the front windows and staring out at the sea. Last week we had much action here when a "micro light" went missing over the sea just in front of our flat! East Coast Radio had a minute to minute update, sirens were going off as the police vans arrived and an ambulance was on stand by and the masses were gathering. Two helicopters searched for hours!
Several hours later the only thing that had been picked up out of the sea was a big piece of plastic. No body had been found, no one was reported missing. Although it was a waste of resources and time it was wonderful to see that if we did have an emergency such as this, our emergency services would cope very well.
What do I do with all this precious free time?...I get into my sewing room and I SEW! I have the radio on so that I can keep abreast with what is happening in the world, and while listening to sweet music , I SEW! Needless to say I am very happy.
To start off I have a couple of U.F.Os that I need to finish and a couple of requests for quilts to make to. Let me begin at the beginning.
A friend, Anita, moved to New Zealand. Before leaving Anita had the task of clearing out her late Mother's things. She came across a quilt top and a quilt backing plus lots of bits and pieces of fabrics. Anita packed them all up and sent them to me with a request to make TWO quilts for her daughters. I looked at Granny's efforts and wasn't sure how I was going to approach this challenge. I asked the opinions of two of my quilting friends and the first one shook her head and said she would never take on the challenge. The second friend was constructive and gave me a little advice and much encouragement.
With all my travels it was a LONG time before I could actually begin on these challenges. Bless Grandma.....the whole time I was working on these quilts I tried to get in her mind and have good positive thoughts.
The back of the quilt consisted of pieces of Sanderson Linen from a book of samples. I pulled the pieces apart, washed them recut the fabrics and put them together with a lovely dusky pink sashing.
I am very pleased with the final result, I hope Anita is too. Now for the bigeye
The second quilt is a greater challenge as Granny had designed the top and had not got it anywhere near completion. I removed all the papers from the hexagonals, did some mending, added more hexies etc and then to cut a long story short, I managed to finish the quilt top and got it quilted to the backing.
After .....finished! I do enjoy the sense of achievement when the last stitch is stitched!
I asked Anita to tell me a little about her Mum and this is the info she sent.
Full names: Thora Sylvia Noome (Meyer)
Born;15August,1922 in Benoni.
She preferred to be called Grandma, loved all arts and her hands were always busy. Grandma used a Bernina treadle machine and loved creating.
I hope I have done Grandma proud and that her Grand daughters will enjoy and treasure these quilts.
I do like to be beside the seaside.....but there are no brass bands playing. There are vendors....
Posted by Penny Boden at 1:06 PM 1 comment:
Labels: ufos, whales


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The Value Of Cheerfulness: November 2010
{ 30 Days of Gratefulness: # 16 }
Our beloved Mama B. (David's Mom) who's birthday is today. I doubt there has ever been a dearer soul on this earth. ( Except for maybe her mother) I cannot even begin to tell you the blessing she is in our lives or how much we adore + admire her. She's makes Nov. 16th a beautiful, beautiful day!
Making headway on cleaning the house.
That I was able to get Annabelle out of the dining room closet quickly, after realizing and bursting into tears that she had locked her self in.
Dryers Limited Edition Peppermint Ice Cream. It's seriously, dangerously good. Thanks a lot Andi. ( it's her favorite and I hold her completely responsible for my addiction to it :)
This really, really good sermon on Fear + Anxiety and Jesus. I did not know until today that Jesus said, " fear not..." more than he said anything else.
Annabelle's precious bedtime prayers to: " Dear Jesus, please fix little baby Ella's body so she can live."
The man of my dreams, who just called down the stairs to me, "Chelle, bedtime."
Posted by Chelle at 10:43 PM 4 comments
Thankfuls ( last weeks )
( find this free Thanksgiving printable here )
I have neglected to post an entire weeks work of daily thankfuls, haven't I? Not because there is a lack of things to be thankful for by any means, but it's been extra busy around here this past week. David is home from work now, as paving season is over for the year. We are all as tickled as can be about that, and love these special days with him and plan to make the most of them while we have with him here at home with us, during the lull between paving and winter work.
So in looking back on this happy little week just gone by, I am grateful for:
How Jack soaks up time with his Daddy's and watches David's every move around the house as he puts finishing touches and touch ups on things around here. All day long Jack carries around his " tool-tools" hitting stuff, and I am thankful for our dear little man in the making, even if it means two cracked + broken windows, two days in a row. We are seriously wondering if he has a arm of steel, as we've puzzled over how a little person can break windows with three swings of his plastic toy hammer?
So very thankful to our sweet Jesus for the miracle of little baby Ella, born earlier this week. For the outpouring of prayers and love for her and her dear Mommy, Daddy and two big brothers. And for the sweet hours spent together with really special + gifted friends this week, praying our hearts out and sewing up a little a baby quilt for precious Ella.
This E.Elliot quote: " Of one thing I am perfectly sure: God’s story never ends with ‘ashes." ( taken from These Strange Ashes )
Getting so close to checking off, " hang pictures on the walls " from the to-do list.
Asking Annabelle what her favorite thing of the day was: " the little pink christmas tree with lights that we saw at the store mommy! "
Jack and Annabelle's melt my heart baby crush on this scrumptious little baby boy that we are all so in love with.
Havarti cheese ( my #1 pregnancy craving ) in my fridge to snack on, a gift from previously mentioned " scrumptious baby boy's" nice + pretty Mommy .
Tea partys and matching hoodies and darling little friends.
Sunday morning fresh out of the oven cinnamon rolls before church.
Steaming cups of Trader Joes Vanilla + Cinnamon Black Tea with honey + cream.
Annabelle's adorable new nick name for Jack: Dinky.
This pretty little spot that's: " a shared collection of ideas + links + inspiration for creative, crafty, party throwing, homemade gift loving, and sometimes homeschooling mama/artists... "
Big pots of Potato and Ham + Ham and Bean Soup. Obviously I am on a ham + soup ( with homeade bread ) kick.
Posted by Chelle at 2:39 PM 3 comments
{ Thankful : Days 5 + 6 }
Friday, Nov. 5th :
Making cookies with Anniebelle's to raise money to for our Operation Christmas Child project...
A impromptu date-night of fajitas + flirting...
Anniebelle's fun sleep-over with Auntie Roo...
How David always says to Jack..." oh, he was a good little monkey...but always very curious..."
The golden kaleidoscope of leaves out our windows, spinning downward in a morning sunburst
A entire two hours spent in the craft room. While Jack napped and Annabelle happily cut and pasted a clown and I worked on BLESSED banners.
David stashing a little bag of chocolate covered gummy bears in our cart and kissing me right in the middle of Winco.
Watching this " Random Act of Culture " with tears streaming down my face and some serious goose-bumps. And the email I got back from Grannie Annie after she'd watched it too..." That was SO beautiful! Oh my, I hope that catches on! Oh, I hope they do this here! I think that is a lovely, lovely thing to do and I hope it catches on! It was delightful, hallelujah! Pass it on! It really was delightful! IT's worth your effort!"
Jacks new love: tilapia. He's on a 30 day elimination diet right now. A really strict one and he can only have turkey and white fish for meat. On top of his already, no gluten, dairy, eggs sugar, soy, nuts, most fruits and some veggies, our try-to -figure-out-what-this-kid-isn't-allergic-to diet.
Posted by Chelle at 8:59 AM 2 comments
Pouring out thanks...
For Jack's appointment with a new doctor that was a total answer to prayer and that gives me hope for getting to the bottom of his allergy/skin issues.
Craft night...which included a bunch of wonderful, creative ladies, busy with a fun Advent project, yummy treats, yards and yards of burlap and laughter + chatting until well past midnight.
A vase full of colorful leaves.
For a little shop update.
Sweet baby flutters.
Nov. 4th :
For a sweet Bible study time this morning. I think our little group is starting to feel a little more brave and safe to take things to a deeper, more honest + real level. For a lot of conviction on my part as we read through the chapter in our book: Profile of the Lukewarm. I wanted to jot down some quotes here but after I'd scoured the house for it, I realized that David must have taken it to work with him, which makes me so happy and thankful to know he's been reading it on his own.
Delighted in these three beautiful little girlies and their matching pig-tail braids...another one of this sweet + darling friend's, 1000 + 1 talents.
The silly telling of a bedtime story by Anniebelle's to her Daddy that started like this..." Once upon a time Daddy there was a rabbit who had a tail made out of cheese. And who walked on his ears. And had two bellybuttons for eyes..."at which point they both dissolve into silly, silly giggles.
Thankful especially for this day because it was on this very day 29 years ago that this most amazing + beautiful lady was born. Struggled all day understanding why God would allow her birthday to be turned upside down by something really scary happening to her just as amazing + beautiful little daughter. All day + evening I worried and ached for them and for other dear friends who are hurting...and then came David's gentle encourage-ment to " not doubt God ...to just trust that He knows and allows what's best for us and those we love so dearly. And by last night the prayers of so many were already being sweetly answered, worries stilled and from the sound of things, it has been a really happy, normal, celebrating + making up for lost time, sort of day for them.
Posted by Chelle at 11:03 AM 6 comments
Labels: 30 Days of Gratefulness
Posted by Chelle at 1:04 AM 4 comments
Posted by Chelle at 12:33 PM 10 comments

type: global

value: Blogger is an American online content management system (CMS) which enables multi-user blogs with time-stamped entries. Pyra Labs developed it before being acquired by Google in 2003.


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23/03/2016 7:01 PM IST
Mukund Padmanabhan will succeed Malini Parthasarathy as the editor-in-chief of The Hindu, becoming only the second editor from outside the founding family of the 127-year-old daily. The decision was made at a board meeting of the Chennai-headquartered publication. Padmanabhan has been working with the group for the past 15 years and headed Businessline since 2012. Business Standard reports: "The decision to appoint him as the new editor was taken at Kasturi & Sons Ltd (KSL's) Board meeting today, according to sources in KSL. Board of Directors and management officials were not available for comment immediately."
In January this year, Malini Parthasarathy, the first woman editor of The Hindu resigned, allegedly over differences with the board regarding the newly-launched edition in Mumbai.
The board of Kasturi and Sons Ltd. (KSL), publishers of The Hindu, appointed Parthasarathy as editor-in-chief of the daily at its meeting on January 20, 2015. She took charge on February 1, 2015.
Following Parthasarathy's exit, Suresh Nambath, the national editor of the paper, was overlooking its daily operations. Padmanabhan, a LSE graduate, had taught in the Delhi University for a brief period before beginning his career in journalism. He had worked with The Indian Express before joining The Hindu.

value: HuffPost, formerly The Huffington Post until 2017 and sometimes abbreviated HuffPo, is an American news aggregator and blog, with localized and international editions. The site offers news, satire, blogs and original content, and covers politics, business, entertainment, environment, technology, popular media, lifestyle, culture, comedy, healthy living, women's interests and local news featuring columnists.


text:
Patent US5919712 - Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsMethods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor...http://www.google.com/patents/US5919712?utm_source=gb-gplus-sharePatent US5919712 - Apparatus and methods for multi-analyte homogeneous fluoro-immunoassaysAdvanced Patent SearchPublication numberUS5919712 APublication typeGrantApplication numberUS 08/748,687Publication dateJul 6, 1999Filing dateNov 13, 1996Priority dateMay 18, 1993Fee statusPaidAlso published asUS6316274, US6979567, US20020160535Publication number08748687, 748687, US 5919712 A, US 5919712A, US-A-5919712, US5919712 A, US5919712AInventorsJames N. Herron, Douglas A. Christensen, Hsu-Kun Wang, Karin D. Caldwell, Vera Janatova, Shao-Chie HuangOriginal AssigneeUniversity Of Utah Research FoundationExport CitationBiBTeX, EndNote, RefManPatent Citations (51), Non-Patent Citations (42), Referenced by (110), Classifications (51), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetApparatus and methods for multi-analyte homogeneous fluoro-immunoassays
The embodiment of FIG. 1 further includes a 45� angle mirror 110 which is positioned for making beam 102 a vertical beam prior to focussing the beam onto the biosensor. It will be understood by those skilled that the number and arrangement of mirrors 104, 106, 108, 110 may be varied as necessary to accommodate various space limitations, with the sole requirement being that a sufficient amount of light be directed to biosensor 120.
Of further interest in FIG. 4A is the orientation of lenses 404, 406 with respect to waveguide 302. It will be seen that the curved edges 404A, 406A face towards waveguide 302, which is 180� from the orientation depicted in FIG. 3C. While the focussing lens can be oriented in either way, the arrangement of FIG. 4A is presently preferred for illumination of the waveguide with the flow-through cell.
In a further and highly preferred embodiment of the biosensor depicted in FIGS. 12A and 12B, the end of the planar waveguide which is distal to the receiving edge, has a portion 650 of serrated or toothed shape. The angles 652A, 652B must be less than the critical angle for total internal reflection at the waveguide-air interface (the critical angle for polystyrene/air is about 51�). Preferably, the sum of angles 652A, 652B should be 90�, so that the light is retro-reflected back along the longitudinal axis of the waveguide; still more preferably, angle 652A=652B=45�. An advantage of this shape is that it increases the level of internal reflection without a reflective coating on the edges, thus reducing manufacturing complexity and costs.
FIG. 19 shows data for the fluorescence intensity of biotin-conjugated Cy5 dye bound to an avidin-coated silica waveguide (ch. A) as a function of beam entry angle, as compared to the "background" level of fluorescence from Cy5 dye free in solution (not conjugated to biotin and therefore not bound to the avidin-coated surface). The critical angle for TIR at the silica-aqueous solution boundary is about 26�. The data of FIG. 19 indicate that the signal-to-background ratio (ch.A�ch.B) for a beam entry angle of 20� to 25� is about 4-fold higher than for 0�. Thus, beam entry angles of one to about five degrees less than the critical angle are presently preferred for silica waveguides. For a polystyrene waveguide with an aqueous adjacent medium, the critical angle for TIR is about 33�, and a useful range of beam entry angles is from about 25� to about 32�, with the higher angles generally being preferable.
Silica chips or glass or quartz microscope slides were cleaned with chromic acid, then treated with 5% APS/95% deionized water (v/v) for about fifteen minutes at room temperature. The APS-treated surfaces were rinsed with deoionized water and absolute ethanol, and incubated in a vacuum oven which had been flushed at least three times with nitrogen, at 120� C. for 1 hour. The resulting silanized surfaces were then soaked in 2.5% glutaraldehyde (E.M. grade from Polysciences) in 0.1M carbonate-bicarbonate buffer, pH 9.2, for two hours at room temperature.
Next, linear PMahy was reacted with the aldehyde groups on the treated chips to create a cross-linked polymer film with many unreacted hydrazido groups in the chains. This was done by dipping the treated chips in solutions of PMahy of between about 5% and 8% (w/v), pH 5.2, at a temperature between about room temperature and about 60� C., for a time sufficient to form a polymer film of thickness about 100 Å or less. The thickness of the hydrogel layer increases with time and temperature of incubation in the solution. It was found that optimal conditions for preparation of the film of 100 Å thickness or less, comprised incubating in 5% (w/v) PMahy for 2 hours at room temperature (about 25� C.).
Following derivatization with SMCC, the hydrogel-coated surfaces were treated with a 1 mg/ml solution of Fab' fragments in phosphate buffer, ph 6.0, with 5 mM EDTA. The waveguide surface so prepared was shown to immobilize Fab' molecules at a surface density of about 1.4�10-12 moles/cm2. Also the surface was able to immobilize Fab' fragments at their C-terminal thiol groups in a site-specific way. The thickness of the resulting polymer film was determined by ellipsometry to be about 100 Å, as was desired. This film thickness is much less than typical previous polymeric films, which have thicknesses of 0.35 to 25 μm (microns). The above-described method of preparing the PMahy polymers is superior to that described by von Kern et al. using polymethacryloylacid esters. Such esters suitable for reaction with hydrazine hydrate often have a molecular weight of 80,000 daltons or more, from which it is difficult to obtain a desirably thin film on the waveguide.
In actual experiments, the procedure was as follows. Chromic acid-cleaned silica surfaces were immersed in a solution of 3�10-6 M (molar) avidin for about 3 hours at room temperature. The surfaces were then washed several times in PBS to remove unadsorbed avidin.
An alternate method was used for biotinylating whole antibodies, in which biotin-LC-hydrazide was coupled to oxidized carbohydrate groups in the Fc region of the antibody. Mab designated 9-40 (a murine monoclonal IgG1 antibody that binds fluorescein), was oxidized by incubation at a concentration of 1-2 mg/ml protein in 10 mM sodium periodate, 0.1 M sodium acetate pH 5.5 for 20 minutes at about 0� C. Glycerol was then added to a final concentration of 15 mM to quench the reaction, and the mixture incubated a further 5 minutes at 0� C. The oxidized Mab 940 was purified by gel filtration chromatography on Sephadex G25 equilibrated with 0.1M sodium acetate buffer pH 5.5, and then reacted with 5 mM biotin-LC-hydrazide for 2 hours at room temperature with agitation. Unreacted biotin-LC-hydrazide was removed using a Sephadex G25 column equilibrated in PBS.
The density of immobilized Fab' fragments obtained using the avidin-biotin coupling chemistry was about 1.4�10-12 moles per cm2 (square centimeter).
The modified PEG was coupled to silanized-glutaraldehyde-treated waveguide surfaces prepared as described in Example I. A solution of 24 milligrams (mg) of PEG-ED powder was dissolved in 1.2 milliliters (ml) of 0.15M PBS pH 7.4 or in the same volume of 11% potassium sulfate-sodium acetate buffer at pH 5.2. The prepared waveguide surfaces were immersed in the PEG-ED solution and incubated at 60� C. for about 24 hours. The procedure using K2 SO4 -acetate buffer yielded a higher density of PEG molecules attached to the surface than that using PBS buffer. Antibodies or other binding proteins were immobilized to the PEG-coated waveguides as follows. A solution of about 3 mg/ml of antibody was dissolved in 0.15 M sodium acetate buffer, pH 5.2. A solution of equivalent weight of 50 mM sodium metaperiodate (NaIO4) was then added, and the reactants were agitated at room temperature for about an hour. Unreacted sodium metaperiodate was removed by passing the reaction mixture through a desalting column (type PD-10 from Pharmacia), which had been pre-equilibrated with the sodium acetate buffer.
Absolute antigen binding was determined by means of radiolabelled tracers or capture molecules. For example, radiolabelled BSA-FL9 was allowed to be coupled with immobilized Fab' fragments for 5 or 60 minutes in phosphate buffer pH 7.3 at room temperature. The tracer concentration was 1.5�10-7 M. Three ml per sample of fluorescein-labelled BSA (BSA-FL9) at concentrations ranging from 10-10 M to 10-7 M was injected into the flow cell. The injection was performed over a five-minute interval. The spectrum at wavelength of 488 nm was taken and the bulk BSA-FL9 was removed by flushing with PBS buffer. Three more spectra were taken, and the fluorescein peak from 513 to 517 nm was integrated. These values were set versus the log of BSA-FL9 concentration in order to obtain the binding isotherm.
TABLE I__________________________________________________________________________Summary of Solid-Phase Immunoassays using Silica Substrates                          Absolute                    Total Non-specific                                Relative                                      Immobilized                    Binding3                          Binding4                                Non-specific                                      Antibody5                                            Specific               Coupling                    (� 10-12)                          (� 10-12)                                Binding                                      (� 10-12)                                            Activity6Surface1       Antibody2               Chemistry                    (moles/cm2)                          (moles/cm2)                                (%)   (moles/cm2)                                            (%)__________________________________________________________________________Hydrophobic Silica (DDS)       Heat Treated IgG               Random                    0.65  0.04  6.46  3.00  21.67Hydrophobic Silica (DDS)       Acid Treated IgG               Random                    0.67  0.07  9.70  2.20  30.45Hydrophobic Silica (DDS)       Fab' Fragment               Random                    0.37  0.25  69.00 1.30  28.46Silica/APS/GLU       Acid Treated IgG               Random                    0.55  0.21  38.18 3.75  14.67Silica/APS/GLU/PEG       Oxidized IgG               Specific                    0.56  0.11  19.64 2.06  27.18Silica/APS/GLU/PEG       Oxidized IgG               Random                    0.41  0.10  24.39 1.44  28.47Silica/Avidin       Biotin-IgG               Specific                    0.72  0.02  2.92  0.94  76.60Silica/Avidin       Biotin-Fab               Specific                    0.84  0.02  2.62  1.10  76.36Silica/Avidin       Biotin-Fab               Specific                    0.80  0.02  1.88  "     72.73(with biotin-PEG)Silica/Hydrogel       Oxidized IgG               Specific                    0.17  0.01  6.88  7.95  2.14(preswollen)Silica/Hydrogel       Fab'    Specific                    1.51  0.03  2.03  2.76  54.71(preswollen)       Fragment__________________________________________________________________________ 1 Abbreviations: DDS  dichlorodimethylsilane; APS  aminopropylsilane GLU  glutaraldehyde; PEG  polyethylene glycol (3400 MW); BSA  bovine seru albumin; IgG  intact immunoglobulin G; Fab' antigen binding fragment with reactive thiol group; ND  not determined 2 All immunoassays were performed with an IgG, monoclonal antibody (9-40) which binds fluorescein. 3 Amount of 125 IFluorescein-BSA which bound to silica substrate. 4 Amount of 125 IBSA which bound to silica substrate. 5 Amount of 125 I9-4O immobilized on silica substrate. 6 Percent of immobilized active sites which bound antigen molecules.
TABLE II__________________________________________________________________________Summary of Solid Phase Immunoassays Using Silica Substrates Coveredwith Hydrogel with Maleimido Reactive Groups            Total hCG                  Total hCG          Relative      Relative      Immobilized            Binding in                  Binding in Absolute Non-                                     Non-specific                                           Absolute                                                   Non-specific Binding      Antibody            5 min 60 min                        Antibody                             specific Binding                                     Binding in                                           specific                                                   Binding in Constant      (�10-12            (�10-12                  (�10-12                        Activity                             in 5 min (BSA)                                     5 min (BSA)                                           in 60 min                                                   60 min (BSA)Antibody pK0      mol/cm2)            mol/cm2)                  mol/cm2)                        (%)  (�10-12 mol/cm2)                                     (%)   (�10-12                                           mol/cm2)                                                   (%)__________________________________________________________________________Fab' from 8.85 1.39 � 0.07            0.62 � 0.03                  0.81 � 0.03                        58.3 � 0.8                             <0.01   <0.97 <0.02   <2.47Anti-hCG-AFab' from 7.89 1.29 � 0.06            0.51 � 0.02                  0.67 � 0.03                        51.9 � 0.1                             0.02 � 0.01                                     3.85 � 1.81                                           0.04 � 0.01                                                   5.91 � 1.22Anti-hCG-BFab' from 8.70 0.74 � 0.04            0.18 � 0.01                  0.41 � 0.02                        55.4 � 0.3                             <0.01   <6.22 0.03 � 0.01                                                   7.21 � 2.09Anti-hCG-CFab' from 8.00 1.45 � 0.06            0.58 � 0.02                  0.75 � 0.03                        51.4 � 0.3                             <0.01   <1.20 <0.02   <3.21Anti-hCG-DFab' from --   2.50 � 0.10            0.04 � 0.01                  0.06 � 0.02                         2.4 � 0.7                             --      --    --      --Mouse IgG__________________________________________________________________________antibody anti-hCG-A was used in the experiments whose results are depicted in FIGS. 7A, 7B, 8, 9A-9F, and 10A-10D.
F(ab')2 fragments were produced by digestion with pepsin using the procedure described by Grey and Kunkel, "H Chain subgroups of myeloma proteins and normal 7S globulin," J. Exp. Med. 120:253-266, 1964. Following digestion, F(ab')2 fragments were reduced to Fab' fragments using dithiothreitol (DTI). Specifically, 33 mg of purified antibody and 1 mg pepsin (Sigma) were dissolved in 0.1 M sodium acetate buffer (pH 4.2) and the digestion was carried out at 37� C. for 16 hours. The digestion was terminated by adjusting the pH of the reaction mixture to 8.0 with 2 M tris base. The F(ab')2 fraction was separated by gel permeation chromatography (Superdex Hiload, Pharmacia) using phosphate-buffered saline (PBS), pH 7.7, as eluent. Fab' fragments were prepared by reducing the F(ab')2 fragments (1 mg/ml) with 1.75 mM DTT and 3.5 mM ethylenediamine tetraacetate (EDTA) in 0.17 M tris buffer (pH 7.4) for 45 minutes at room temperature. After reduction, excess DTT was removed by gel permeation chromatography using a Sephadex G-25 column (Pharmacia) equilibrated in 0.1 M sodium phosphate buffer (pH 6.0) containing 5 mM EDTA.
TABLE III__________________________________________________________________________Summary of Solid Phase Immunoassay Using Silica Substrates withAdsorbed Avidin and Biotinylated Fab` Fragments                            Absolute      Immobilized              Total   Specific                            Non-specific Binding                                      Relative      Antibody              hCG Binding                      Activity                            (BSA)     Non-specific BindingAntibody   (�10-12 mol/cm2)              (�10-12 mol/cm2)                      (%)   (�10-12 mol/cm2)                                      (BSA) (%)__________________________________________________________________________Fab' from Anti-hCG-A      1.19 � 0.02              1.22 � 0.01                      100 � 5                            0.05 � 0.02                                      4.20 � 0.02Fab' from Anti-hCG-B      1.40 � 0.05              1.38 � 0.07                      98 � 9                            0.05 � 0.01                                      3.57 � 0.02Fab' from Anti-hCG-C      2.24 � 0.02              1.10 � 0.03                      49 � 3                            0.05 � 0.01                                      2.32 � 0.01Fab' from Anti-hCG-D      1.59 � 0.02              1.24 � 0.01                      78 � 2                             0.05 � 0.005                                      3.14 � 0.01Fab' from Mouse IgG      1.25 � 0.02               0.03 � 0.003                       2.4 � 0.05                            0.09 � 0.03                                      7.20 � 0.03__________________________________________________________________________
From the data in Tables IV and V, it is evident that the degree of nonspecific binding was significantly lower for the PF108 and PP105 coatings than for PF68 and PF88, and with Fab' concentrations of 1.5�10-6 M. For this reason, PF108 and PP105 are presently preferred BCPs. In general, among TBCP compounds, those exhibiting better resistance to non-specific binding (and thus presently preferred) are those having PPO segments of length about 45-50 residues or more. Desirably, the level of non-specific binding should be no more than about 10%, and preferably below about 1%-2%, of the level of specific binding. Alternately, or in addition, it is desirable that the absolute amount of non-specific binding be in the range below about 5�10-14 and preferably below about 5�10-15. Neither the hydrophilic-to-lipophilic balance of the TBCP, the total MW, or the molecular weight ratio of PEO to PPO in the compound, appear to be as important as the length of the PPO segment in selecting TBCPs with good efficiency in inhibiting non-specific binding.
TABLE V______________________________________Effects of Pluronics Coating on the Non-SpecificBinding of BSA and Fluorescein-BSA to PolystyreneExperi-         Pluronics                    Fluorescein-ment            Coating  BSA       BSANo.   Pluronics Time     (mol. cm-2)                              (mol. cm-2)______________________________________1.    None      --       6.05e-12  8.37e-132.    F108       24 hours                    5.46e-14  8.13e-153.    None      --       1.96 � 0.04e-12                              1.53 � 0.06e-124.    P105       24 hours                    <1e-15    <1e-155.    F68        24 hours                    1.68 � 0.30e-13                              0.91 � 0.32e-136.    F88        24 hours                    1.03 � 0.30e-13                              0.32 � 0.17e-137.    F108       10 min  <1e-15    <1e-158.    F108       30 min  <1e-15    <1e-159.    F108       60 min  <1e-15    <1e-1510.   F108      180 min  <1e-15    <1e-1511.   F108       24 hours                    <1e-15    <1e-15______________________________________
Silica-MSil(5000) surfaces were prepared by incubating silica chips for 40 minutes at 90� C. with a 10% aqueous solution of MSil(5000). Silica-DPS-PF108 surfaces were prepared by immersing the silica chips for 60 minutes at room temperature in a solution of 10% DPS in toluene. The DPS surfaces were washed with ethanol, then with water, and then immersed in a 4% aqueous solution of PF108 for about 24 hours at room temperature.
Silica-APS-Glu-PEG(2000) surfaces were prepared by incubating silica chips with an aqueous solution of 10% APS at room temperature for 30 minutes. The APS-coated chips were then washed with ethanol and with water, and then immersed in 2.5% aq. solution of glutaraldehyde in bicarbonate buffer, pH 8.0, for 2 hours at room temperature. The chips were washed again, and then reacted with methoxy-PEG(2000) hydrazide in acetate buffer containing 11% K2 SO4, pH 5.2 at 60� C. for about 24 hours. Silica-avidin-biotin-PEG(3400) surfaces were prepared by incubating silica chips with avidin (3�10-6 M in PBS) at room temperature for 3 hours, then washed with PBS. The
avidin-coated chips were then reacted with biotin-PEG(3400) (also 3�10-6 M in PBS) for 3 hours at room temperature.
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Archaeolibrarian - I dig good books!: COVER REVEAL - Heart on Fire by Brandy L Rivers
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Patent US6533719 - Diaphragm based spontaneous inflation inhibitor in a pump for an inflatable ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsA pump assembly for a penile implant is provided having a mechanism which prevents spontaneous inflation of the cylinders implanted within the user. The preventative mechanism uses overpressure generated by the reservoir during unintentional compression to effectively seal the pump assembly from unintended...http://www.google.ca/patents/US6533719?utm_source=gb-gplus-sharePatent US6533719 - Diaphragm based spontaneous inflation inhibitor in a pump for an inflatable prosthesisAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS6533719 B2Publication typeGrantApplication numberUS 09/749,256Publication date18 Mar 2003Filing date27 Dec 2000Priority date27 Dec 2000Fee statusPaidAlso published asUS6935847, US7350538, US20020091302, US20030065249, US20050250981Publication number09749256, 749256, US 6533719 B2, US 6533719B2, US-B2-6533719, US6533719 B2, US6533719B2InventorsCharles C. Kuyava, John W. Westrum, Jr., David W. ClarkOriginal AssigneeAms Research CorporationExport CitationBiBTeX, EndNote, RefManPatent Citations (60), Referenced by (80), Classifications (11), Legal Events (15) External Links: USPTO, USPTO Assignment, EspacenetDiaphragm based spontaneous inflation inhibitor in a pump for an inflatable prosthesis
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W.;CLARK, DAVID W.;REEL/FRAME:011711/0551;SIGNING DATES FROM 20010320 TO 2001032221 Dec 2001ASAssignmentOwner name: AMERICAN MEDICAL SYSTEMS, INC., MINNESOTAFree format text: RE-RECORD TO CORRECT THE FIRST INVENTOR S NAME, PREVIOUSLY RECORDED AT REEL 011716, FRAME 0134.;ASSIGNORS:CABAK, JAMES E.;WESTRUM, JR., JOHN W.;CLARK, DAVID W.;REEL/FRAME:012400/0316;SIGNING DATES FROM 20010320 TO 200103266 Dec 2002ASAssignmentOwner name: AMS RESEARCH CORPORATION, MINNESOTAFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:AMERICAN MEDICAL SYSTEMS, INC.;REEL/FRAME:013282/0811Effective date: 2002120623 Jun 2003ASAssignmentOwner name: BANK OF AMERICA, N.A., AS AGENT, NORTH CAROLINAFree format text: NOTICE OF GRANT OF SECURITY INTEREST;ASSIGNOR:AMS RESEARCH CORPORATION;REEL/FRAME:014186/0264Effective date: 2000041729 Jun 2006ASAssignmentOwner name: AMS RESEARCH CORPORATION, MINNESOTAFree format text: RELEASE BY SECURED PARTY;ASSIGNOR:BANK OF AMERICA, N.A., AS 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text: Patent US5298784 - Electrically programmable antifuse using metal penetration of a junction - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Advanced Patent Search | Sign inAdvanced Patent SearchPatentsAn improved antifuse uses metal penetration of either a P-N diode junction or a Schottky diode. The P-N junction, or Schottky diode, is contacted by a diffusion barrier such as TiN, W, Ti-W alloy, or layers of Ti and Cr, with a metal such as Al. Al-CU alloy, Cu, Au, or Ag on top of the diffusion barrier....http://www.google.com/patents/US5298784?utm_source=gb-gplus-sharePatent US5298784 - Electrically programmable antifuse using metal penetration of a junctionPublication numberUS5298784 APublication typeGrantApplication numberUS 07/858,835Publication dateMar 29, 1994Filing dateMar 27, 1992Priority dateMar 27, 1992Fee statusLapsedAlso published asEP0562996A1Publication number07858835, 858835, US 5298784 A, US 5298784A, US-A-5298784, US5298784 A, US5298784AInventorsJeffrey P. Gambino, Dominic J. Schepis, Krishna SeshanOriginal AssigneeInternational Business Machines CorporationPatent Citations (16), Non-Patent Citations (6), Referenced by (70), Classifications (13), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetElectrically programmable antifuse using metal penetration of a junctionUS 5298784 AAbstract An improved antifuse uses metal penetration of either a P-N diode junction or a Schottky diode. The P-N junction, or Schottky diode, is contacted by a diffusion barrier such as TiN, W, Ti-W alloy, or layers of Ti and Cr, with a metal such as Al. Al-CU alloy, Cu, Au, or Ag on top of the diffusion barrier. When this junction is stressed with voltage pulse producing a high current density, severe joule heating occurs resulting in metal penetration of the diffusion barrier and the junction. The voltage drop across the junction decreases by about a factor of ten after the current stress and is stable thereafter. Alternatively, a shallow P-N junction in a silicon substrate is contacted by a layer of metal that forms a silicide, such as Ti, Cr, W, Mo, or Ta. Stressing the junction with a voltage pulse to produce a high current density results in the metal penetrating the junction and reacting with the substrate to form a silicide.


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fashion rochester: Things My Mother Never Told Me: Makeup
Anything creamy or liquid should go on before dry, powdery products. This means, eye liners (creamy) before shadow (powder). Common sense- the shadow SETS the liner and keeps it from traveling. THEN, if need be, go back and add an accent or two atop your shadow. But, take it from my trial and error, it is annoying when liquid liner goes skidding across already powdered skin. 2. Makeup: Curl Lashes BEFORE Mascara Honestly, you are just wasting mascara and giving yourself more cleanup to do if you apply before curling. Avoiding curling altogether by priming the lashed instead.
3. Makeup: Primer, What is It? It is what it sounds like. It's just like balancing and prepping your walls for a new paint job. Skin Primer goes on after your moisturizer and before your foundation to even out skin discoloration and make your makeup last hours and hours longer without constantly touching it up. A 20th century gift to woman.
Foundations with SPF of 20 or higher can create glare ("shine") when reflecting light of a camera. Stick to SPF 15 or lower in your daily lotions and foundations. Better yet, steer yourself in the direction of foundations MADE to be used under lights. Andrea's Top Picks: Foundation and Mascara
Mascara: Lancome Primer and classic Definicils Mascara. Naturally defined lashes... I try to cheat on Definicils, but find myself going back again and again, dissatisfied with so-called competitors. Photo Credits: Photo by Hannah Betts, Hair/Makeup by myself, Andrea Galdieri Bonawitz, Model Anna Dana