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CA2508001A1 - Systems and methods of sample processing and temperature control - Google Patents
Systems and methods of sample processing and temperature control Download PDF
CA2508001A1
CA2508001A1 CA002508001A CA2508001A CA2508001A1 CA 2508001 A1 CA2508001 A1 CA 2508001A1 CA 002508001 A CA002508001 A CA 002508001A CA 2508001 A CA2508001 A CA 2508001A CA 2508001 A1 CA2508001 A1 CA 2508001A1
CA002508001A
CA2508001C (en
2002-12-20 Priority to US43560102P priority Critical
2002-12-20 Priority to US60/435,601 priority
2003-12-22 Application filed by Dakocytomation Denmark A/S, Kristopher Buchanan, Marc Key, John Favuzzi, Rosanne Welcher, Benno Guggenheimer, Robert Clark, Michael Barber, Bob Lathrop, Dako Denmark A/S filed Critical Dakocytomation Denmark A/S
2003-12-22 Priority to PCT/US2003/040880 priority patent/WO2004059287A2/en
2004-07-15 Publication of CA2508001A1 publication Critical patent/CA2508001A1/en
2019-03-19 Publication of CA2508001C publication Critical patent/CA2508001C/en
SYSTEMS AND METHODS OF SAMPLE PROCESSING
The present invention is directed to the field of sample processing.
Embodiments of the invention may especially relate to temperature control. Specifically, the present invention relates to temperature control in relation to sample processing systems and methods of processing samples, and may be directed to sample processing in relation to sample carriers and processing materials such as reagents. Scientific fields to which the present invention may have particular applicability include immunohistochemistry, in-situ hybridization, fluorescent in-situ hybridization, special staining, such as special staining of histological samples, microarray sample processing, and cytology, as well as potentially other chemical and biological applications.
BACI~GROLTND OF THE INVENTION
The sample processed may be any material, but is most likely a biologic material such as a biological sample or a biological specimen, perhaps such as a histological sample, e.g. tissue and cell specimens, cells, collections of cells, or tissue samples, the definition to include cell lines, proteins and synthetic peptides, tissues, cell preps, cell preparations, blood, bodily fluids, bone marrow, cytology specimens, blood smears, thin-layer preparations, and micro arrays. It should also be understood to include slide-based biological samples. In preparation for biologic sample analysis, for example, a biological sample may be acquired by known sample acquisition techniques and may comprise, for example in immunohistochemistry (IHC) applications, tissues generally or even in some applications one or a plurality of isolated cells, such as in microarray samples, and may be presented on a sample Garner such as a microscope slide. Furthermore, the sample may be presented on the carrier variously and potentially in some form of preservation.
As one example, a sample such as a layer or slice of tissue may be preserved in formaldehyde and presented on a carrier with one or more paraffin or other chemical layers infiltrating the sample.
IHC applications, for example, may require processing sequences or protocols that comprise steps such as deparaffinization, target retrieval, and staining, especially for in-situ hybridization (ISH) techniques. Important for many IHC applications, and many sample processing sequences and protocols, generally, are temperature characteristics associated with the sample, sample carrier, and the processing environment. As but one example, stains such as histochemical reagents are typically used to identify various histological features. The reagents may employ antibodies, for example, that bind to specific proteins of the sample. In many processes, a need can exist for adequate control of processing characteristics such as temperature. In regard to staining, it should be understood that the term staining can reference the end product of the process, by which certain parts of the sample may be stained, i.e. have obtain a different color, either in the optic range or in another electromagnetic range, such as ultra violet.
Staining may be detectable, perhaps automatically detectable, through some change in properties, such as fluorescent properties, magnetic properties, electrical properties or radioactive properties.
Staining a sample can involve a series of treatment steps, such as washing, binding of reagents to the specific parts of the sample, activation of the reagents, etc.
Sample processing with the reagents may require the addition and removal of reagents in accordance with a defined protocol that may include,a defined temperature.
Traditional sample processing technology has provided temperature control through heating devices that heat an entire set of sample carriers in the sampling processing system. Other technologies, such as the sample processing system described in U.S. Patent No. 6,183,693, may provide heating devices for individual sample carriers that are individually controlled to heat the slides. However, each of these traditional sample processing systems may lack a desired degree of temperature control or temperature tolerances.
2 Inadequacies in temperature control of traditional technologies may include uncontrolled cooling. Traditional systems may only provide ambient cooling when the heating devices are off. Ambient cooling is not considered active control and may not meet protocol temperature requirements or may not otherwise be optimal.
Although heating and heat control may be features of such systems, controlled cooling of the samples, sample Garners, and processing environments may not always be adequately addressed. Cooling techniques such as hooded fans may be incorporated in some traditional technologies. However, these devices can lack sufftcient capabilities of temperature control to meet certain protocol requirements, especially temperature tolerances for samples, sample Garners, reagents, and ambient system temperature.
Traditional systems may even lack temperature control, perhaps as related to temperature tolerances generally, as such tolerances may not be adequately maintained during ambient or other traditional cooling, or during processing sequences or events, generally. In some protocols, for example, the temperature tolerances during non-heating periods may be such that uncontrolled temperature changes may produce undesirable results during the processing sequence. Other IHC processes of the protocol may be adversely affected by uncontrolled temperature changes, the degree of temperature change, and temperature changes outside of preferred tolerances.
The lack of temperature control may actually dissuade technologists from employing preferred processing sequences or protocols, especially IHC sequences that may be dependent upon a particular temperature tolerance and the amount of temperature change during a processing sequence.
Certain types of temperature control may not have even been addressed in traditional sample processing system technologies. As previously mentioned, reagents can play a vital role in the staining sequence of many processing protocols.
The quality of the reagents, therefore, may be important for adequate sample processing.
Reagents, for example, can have a certain shelf life that may be limited if maintained at undesirable temperatures such as the typical ambient temperatures of traditional processing systems and the laboratories housing such systems. Traditional technologies may lack the temperature control needed to optimally preserve the reagents stored in the processing system that are often subject to inadequate or changing ambient temperatures of such systems and the laboratory environment.
3 Previously, in some traditional processing sequences, protocol steps may have been performed manually, potentially creating a time-intensive protocol and necessitating personnel to be actively involved in the sample processing. Attempts have been previously made to automate sample processing to address the need for expedient sample processing and a less manually burdensome operation. However, such previous efforts may have not fully addressed the needs for an automated sample processing system.
Previous efforts to automate sample processing may be especially deficient in several aspects that prevent more robust automated sample processing, such as: the lack of sufficient temperature control and temperature monitoring associated with sample processing, and the lack of real-time, lack of active, or lack of adaptive temperature control capabilities for multiple sample batch processing. As but one example, the lack of controlled cooling features of traditional systems may require longer wait times for the technologist during processing sequences to allow samples, sample carriers, and ambient temperatures to reach certain protocol temperatures.
Sample processing and temperature control can be accomplished to address the inadequacies of previous sample processing technology. The sample processing and temperature control features of the present invention are addressed in a fashion that may provide the processing of one or more batches of samples and carriers with common protocols or of a plurality of groups of one or more samples and carriers having differing processing protocols. Processing may in a occur sequential or non-sequential,fashion.
Processing of samples and temperature control may be determined by the protocol to be followed for each sample or a protocol for multiple samples. Aspects of the present
4 invention may be especially applicable to sample processing having one or a plurality of processing steps to be performed on one, a portion, or an entirety of samples.
Protocols may include certain temperature tolerances for samples and system components such as samples, Garners, or reagents. There may be temperature tolerances that may be necessary for some sample processing sequences. Aspects of the present invention may be especially applicable to IHC techniques, as well as in-situ hybridization (ISH) and fluorescent in-situ hybridization (FISH), special staining of histological samples, and microarrays; and especially techniques, generally, incorporating deparaffinization and/or target retrieval and/or the staining of samples. Furthermore, embodiments may be especially directed to processing sequences addressing issues of temperature control and data acquisition related thereto.
Embodiments of the invention addressing temperature control may comprise:
sample Garner temperature regulation systems; sample carrier temperature regulation systems configurable with one or a _ plurality of sample carrier supports; and corresponding methods of sample carrier temperature regulation. Embodiments may also include: reagent temperature regulation systems; reagent temperature controls;
5 reagent temperature regulation systems; and corresponding methods of reagent temperature regulation.
In some embodiments, an automated sample processing system is disclosed comprising a plurality of drawers, a plurality of sample Garner retainment assemblies each removably configured with one of the drawers, a temperature regulation system, such as a temperature regulation device, and an adaptive sample processing control system to which the drawers, the sample carrier retainment assemblies, and the temperature regulation system may be responsive. An adaptive sample processing control system may automate the sample processing system such that one or more batches of samples may be adaptively processed according to one or more protocols, especially accordingly to temperature requirements of the protocol(s), potentially indicated by information on the slides that may be automatically identified by the sample processing control system perhaps through a camera or the like. Sample processing may comprise one or more sampling protocols and steps, such as deparaffinization, target retrieval, and staining, and the temperature requirements for each, such as their temperature tolerances.
As mentioned, sample processing temperature may be achieved to adequately maintain or change temperatures within protocol tolerances. Accordingly, in some embodiments, temperatures of the sample, sample Garner, or ambient system temperature, or combinations thereof, can be changed in a controlled fashion to achieve ramping temperature increases and decreases (and thus considered as having a temperature ramp up element or a temperature ramp down element, respectively), can have preferred tolerances, can minimize changes of temperature during processing, can maintain reagent quality through temperature control of the reagents, can provide for adaptive heating or cooling, and can control temperatures below or above ambient system or even the ambient lab environment temperature.
Temperature sensing, such as Infrared (IR) or other temperature sensing, may be accomplished in some embodiments, perhaps even by a camera or perhaps a photodiode device. Temperature information, such as ambient system temperature, slide temperature, sample temperature, and reagent temperature, may be identified, and in some preferred embodiments, instantaneously identified. In some embodiments, protocol information, such as required temperature, and required temperature tolerances may be provided. The
7 PCT/US2003/040880 system may include an adaptive sample processing control system or an adaptive temperature regulation element. An adaptive temperature element may include a system that alters or causes a change in the degree or nature of control due to changes in an another component. But one example may be a system that monitors or adjusts temperature more frequently in certain situations, perhaps such as when ambient and desired temperatures have a large spread -- perhaps greater than 5 or 10 degrees C -- or such as when there is an unusual change in ambient -- here perhaps a change of more than 3 or 5 degrees C. The sample processing system may process one or more slides, or one or more batches of slides, concurrently, sequentially, or in any other temporal fashion, potentially in accordance with temperature protocol information provided by a slide having a sample or provided by the adaptive sample processing control system.
Sample batches or individual slides may be inserted or removed during processing protocol steps by the control and monitoring accomplished by the adaptive sample processing control system.
Another embodiment of the present invention may comprise a method of sample processing, comprising the steps of accessing at least one of a plurality of samples or perhaps drawers, providing at least one sample Garner retainment assembly configured with at least one sample carrier, configuring at least one of the drawers with the at least one sample Garner retainment assemblies, utilizing a carrier temperature regulation element and adaptively or actively controlling temperature during processing of the sample Garners. Any aspect of temperature control disclosed herein may of course be combined with any element or elements of such disclosed sample processing systems, or with any of the disclosed features or steps of sample processing.
Many other embodiments of the invention are disclosed in this application, some of which may comprise independently, dependently, or in combination, sample processing systems, environmental control systems, and any of the various other systems, devices, apparatus, assemblies, steps, and features disclosed herein or in the incorporated references of this application. In addition, the various method steps may be provided for individual samples or multiple batch processing, sample diagnostic features, and real-time or adaptive capabilities for multiple batch processing.
Figure 2. is an elevated view of an embodiment of a portion of a sample carrier retainment assembly of one embodiment of the invention.
Figure 8 is a chart providing description of representative deparaf~nization, target retrieval and other steps of an embodiment of the invention.
Figure 9 is a depiction of a networked embodiment connecting one stainer with one manager and one label printer.
8 Figure 10 is a depiction of an embodiment for processing a collection of samples with a collection of reagents according to the invention.
The following descriptions are provided to describe various embodiments of the present invention to facilitate an understanding of the invention. The variously described embodiments should not be construed to limit the present invention to only explicitly described embodiments. Those embodiments and combinations of features inherent in this disclosure or otherwise known to one skilled in the art are also disclosed as the present invention. This description may further be understood to disclose the variously described embodiments of systems, methods, techniques, and applications, both singularly and in various combinations, consistent with the various features of the present invention. Accordingly, the following -is a detailed description of a number of specific embodiments of the invention.
9 Figures 1 and 10 show embodiments of a sample processing system 1 in accordance with the present invention. Cabinet sections ~ 2 form outer portions of the system and serve to address general structural considerations of the system (a top cabinet section is not shown in Figure 1). The sample processing system may comprise a plurality of drawers 4 used for the handling and processing of samples and sample Garners such as slides, potentially microscope slides. Other sample carriers may be accommodated consistent with the present invention. Each drawer may be configured to accommodate sample Garner retainment assemblies, such as slide retainment assemblies, carrier racks, modules, or magazines.
One embodiment of a sample carrier retainment assembly may comprise a slide retainment assembly 6 as shown in Figure 2. The slide retainment assembly may comprise a slide rack, module, or magazine. Slide retainment assembly 6 is configured to accommodate a plurality of slides (only one is shown) in at least one configuration in corresponding sample carrier retention devices 8. The sample Garner retainment assemblies are utilized in the processing of samples as further described below. It should be further noted that the sample carrier retainment assembly can be removably configured with the drawers 4, and may be stackable or nested within other retainment assemblies.
One or more drawers 10 as shown in Figure 1 may be provided to accommodate processing materials such as reagent containers for sample processing. A
processing material retainment assembly, such as a container rack 11, shown in Figure 1, for example, may be utilized to accommodate reagent containers or other processing materials within each of drawers 10. Bottle inserts may be preferably configured with the retainment assembly to ensure proper processing material positioning within the processing material retainment assembly and the drawer.
Multiple drawers 4 as shown in Figure 1, allow for one or a plurality of sample processing protocols to be performed by the system 1. Past efforts at sample processing may have been limited to processing sequences for an entire batch of Garners within the system. The present invention, however, in part by providing a plurality of drawers and Garner retainment assemblies, may allow for multiple batch processing, including real-time alteration capabilities for multiple batch processing, as further described below.
Embodiments of the present invention may further comprise an arm 20, shown in Figure 3, utilized in sample processing, potentially having robotic movement, and in some embodiments, Cartesian movement. The arm 20 may comprise one or more elements, such as an actuator probe 22, a probe such as a syringe 24, a sensor element, an optical sensor 86 (including but not limited to a camera or a CCD device), and even a non-discrete volume fluid and/or air applicator. The optical sensor may even be configured to sense temperature such as through IR detection or the like.
In accomplishing a processing sequence, and in some preferred embodiments of the present invention, slides may be configurable in both vertical and horizontal positions such as for the pretreatment and/or staining processes, as shown in Figures 4A-4D. This may allow for the automation of the pretreatment and staining of slides in various manners. The slides may be initially loaded onto the carrier retention assemblies, such as slide racks, and drawers in the horizontal position. The slides may be horizontally supported by adjustable carrier supports. If ~ pretreatment is required, such as deparaffmization, the system may rotate the slide into the vertical position and may lower these samples into a processing tank which may be maintained at a desired temperature such as perhaps 95 degrees C and may be filled with the required fluids. In some embodiments, the slide rack may be lowered to affect lowering of the slides.
To perform a staining process on the slides, as described below and in some embodiments, the system may rotate the slide to the horizontal position and a syringe or probe may apply fluid to the sample, providing a horizontal staining of the sample. Each slide can be rotated independently allowing for the independent processing of different samples with different requirements.
The sample processing system may further have the ability to maintain and regulate the internal temperature of the system, including maintaining and regulating the temperature of samples and sample earners, to specified temperatures, and even within temperature tolerances of certain sample protocols. Controlling temperature can avoid a need to alter protocols for seasonal or other non-optimal temperature variations. Thermal control may be needed for several heat sources within the system and for temperature effects from outside the system, as well as ambient temperature control of the internal environment of the system. In some preferred embodiments, the internal ambient temperature may be maintained at a set point, perhaps about 24° C, and perhaps ~2° C or ~1 ° C; in other embodiments the sample or reagent temperature may be maintained at about a set point, perhaps about 24° C, and perhaps ~2° C or ~1° C, at about an incremental range, and in some embodiments a non-integer incremental range.
Reagents used in the sample processing system can be optimized to operate at a thermal set point for a substance such as a reagent or sample or for the system in general, and in some embodiments, may be optimized for temperature maintenance at less than about the ambient temperature of the system.
The other heat sink/fan of the pair 80b may be on the outside of the controlled volume, where it is exposed to the ambient environment of the laboratory. One or more thermoelectric devices (TEDs) 81 perhaps including the electrical junctions themselves may be located on the boundary between the interior and exterior. The TED or TEDs may generate a hot portion and a cold portion and may aid in moving heat into or out of the desired location. The "hot" portion may be configured to distribute heat from the exterior of the controlled interior volume. If the temperature of the "hot"
portion of the TED is controlled to maintain a low temperature, such as with a controlled paired heat sink/fan, the corresponding "cold" portion of the TED, may be configured within the controlled interior volume, may be colder by a corresponding amount, and may act in conjunction with a paired heat sink/fan as a controlled refrigerator, and may even actively reduce the temperature of the interior volume, or may achieve protocol tolerances as further described below. Such an item may serve as a temperature reduction element for various locations or purposes as described below.
As mentioned above, the internal temperature of the system may be controlled by an adaptive sample processing control system. Some applications may provide temperatures at 24°C ~2°C; in other embodiments the internal ambient temperature may be maintained at about 24°C comprises ~ an incremental range, such as a non-integer incremental range. One temperature regulation system of the present invention may comprise one or more heat pumps, and in some preferred embodiments two thermoelectric heat pumps (heat pump 80 shown in Figures 6A and 6C). The temperature regulation system may feature each heat pump module having a heat sink and fan on either side of the TED.
Embodiments of the invention may comprise sample carrier temperature regulation systems, in some embodiments sample Garner temperature regulation systems configurable with one or a plurality of sample carrier supports, and corresponding methods of sample carrier temperature regulation. Some embodiments may comprises a Peltier grid, such as grid 60 shown in Figures 5, that may be used to heat or cool the slides during processing of the samples. Thermal elements 62 may heat the slides, in some embodiments from ambient to about 120° C comprises in about 3 minutes. Sample Garner temperature regulation systems may comprise, in some embodiments, one or more sample Garner supports such as a slide support plate 90 as shown in Figure 11, configured with temperature regulation elements, such as one or more temperature regulation elements, and in some embodiments a laminated thermal element 92 as shown in Figure 11, and a cold plate 94 shown in Figure 11.
The various embodiments of the disclosed temperature regulation system and the sample processing control system feature the capability to control system temperature, and in some embodiments, slide temperature and reagent temperature. The combination of features may allow active heating and cooling of sample carriers, and in some embodiments potentially utilizing a controlled Peltier device or temperature control, a conductive device or temperature control, or a combination of temperature control features. One preferred temperature control sequence may allow a controlled (e.g., adjustment or maintenance within a particular set parameters such as rate of change or the like) or even accelerated increase and/or decrease in slide temperature, perhaps including independently a ramping up and/or down of the temperature. The system may be considered as including a controlled temperature element or a controlled active temperature element, such as a controlled active temperature reduction element or the like. Another example of a controlled temperature sequence is shown in Figures 12 and 13. These figures generally illustrate and compare temperature changes of the present invention and a type of traditional system. They illustrate target temperature tolerance, the time necessary to reach values, and ~ ambient temperature aspects. In some embodiments, energy may be delivered at the same or about the same rate by the temperature regulation system as a traditional system. Energy may also, however, be removed or added, and perhaps even removed or added faster or slower than a traditional system, as traditional systems may dissipate energy to the ambient. A shorter or longer period for temperature effects, such as sample carrier cooling, may result.
Active temperature regulation, in some embodiments heating and cooling, may be provided in some embodiments to provide such results.
The various embodiments of the disclosed temperature regulation system may feature the capability of controlling reagent temperature alone or in addition to sample temperature. One embodiment of a reagent temperature regulation system is shown in Figure 14 and may include a conduction temperature regulation system. A
reagent temperature regulation system may have conductive regulation elements 100 perhaps mounted below the reagent tray. The conductive regulation elements may feature thermoelectric regulation features such as Peltier-type temperature regulation. Naturally, a sensing element may be provided as part of arm 20 or in another sample processing configuration, may be incorporated to sense temperature, perhaps instantaneously. This may assist in maintaining temperature tolerances and in controlling rates of temperature change. Photodiode devices, electric conductivity devices, IR sensors, sensors acting through septa of a container, or other sensors may be included to sense values such as reagent containers or slides collectively or individually.
Temperature control of the temperature regulation system may be provided to take advantage of the active heating and cooling capability of the above described temperature regulation system. Accordingly, in some embodiments temperature control may be provided to at least actively regulate temperature within protocol tolerances.
The temperature regulation system of the present invention previously described may be accordingly configured to increase or reduce temperature, and in some embodiments actively increase or reduce temperature. The adaptive sample processing control system may provide a corresponding controlled increase or reduction of temperature, and in some embodiments actively controlled increase or reduction of temperature. It may also reduce the rate of an increase or decrease in temperature change (as compared to the often-used maximum power type of approach) such as by intermittently powering or lower powering the device or the like and may thus provide a reduced rate of temperature change element.
Corresponding methods of the invention may comprise methods of temperature control of sample processing systems, comprising the step of regulating temperature within protocol tolerances, and in some embodiments, actively regulating temperature. Further methods of temperature control of sample processing systems are disclosed comprising one or more steps of actively increasing temperature, actively reducing temperature, or a combination of such steps. A method of temperature control of sample processing systems is further disclosed comprising the step of controlling increase of temperature, controlling reduction of temperature, or a combination of such steps.
Corresponding methods of the invention relate to temperature control of samples, sample carriers, and reagents.
Embodiments of ISH may require a small volume of agent, such as 15 micro liters, to be placed on the sample. Heat control may be maintained between about 95-100°C
comprises and kept constant for a period of time. Temperature may then be lowered in a controlled manner.
As can be easily understood from the foregoing, the basic concepts of the present invention may be embodied in a variety of ways. It involves both sample processing techniques as well as various systems, assemblies, and devices to accomplish sample processing and other functions. In this application, the sample processing techniques are also disclosed as part of the results shown to be achieved by the various systems, assemblies, and devices described and as steps which are inherent to utilization. They should be understood to be the natural result of utilizing the devices as intended and described. In addition, while some devices are disclosed, it should be understood that these not only accomplish certain methods but also can be varied in a number of ways.
Further, each of the various elements and features of the invention and claim may also be achieved in a variety of manners. This disclosure should be understood to encompass each such variation, be it a variation of an embodiment of any apparatus embodiment, a method or process embodiment, or even merely a variation of any element of these. Particularly, it should be understood that as the disclosure relates to elements of the invention, the words for each element may be expressed by equivalent apparatus terms or method terms -- even if only the function or result is the same. Such equivalent, broader, or even more generic terms should be considered to be encompassed in the description of each element or action. Such terms can be substituted where desired to make explicit the implicitly broad coverage to which this invention is entitled. As but one example, it should be understood that all actions may be expressed as a means for taking that action or as an element which causes that action. Similarly, each physical element disclosed should be understood to encompass a disclosure of the action which that physical element facilitates. Regarding this last aspect, as but one example, the disclosure of a "retention element" should be understood to encompass disclosure of the act of "retaining" -- whether explicitly discussed or not -- and, conversely, were there effectively disclosure of the act of "retaining", such a disclosure should be understood to encompass disclosure of a "retention element" and even a "means for retaining". It should also be understood that in jurisdictions where specific language may be construed as limiting, as but one example in the United States where some interpretations of "means for" elements can be construed narrowly, broader equivalent language (such as "
element" or the like) may be used and should be understood as encompassed by this specification. Such changes and alternative terms are to be understood to be explicitly included in the description.
Any acts of patents, patent applications, publications, or other references mentioned in this application for patent are hereby incorporated by reference.
In addition, as to each term used it should be understood that unless its utilization in this application is inconsistent with such interpretation, common dictionary definitions should be understood as incorporated for each term and all definitions, alternative terms, and synonyms such as contained in the Random House Webster's Unabridged Dictionary, second edition are hereby incorporated by reference. Finally, any priority case for this application is hereby appended and hereby incorporated by reference.
1. A method of automated sample processing, comprising the steps of:
determining a processing sequence for at least one sample;
actively regulating temperature of said sample; and automatically processing said sample.
2. A method of automated sample processing as described in claim 1 wherein said step of actively regulating temperature comprises the step of reducing temperature of said sample.
4. A method of automated sample processing as described in claim 2 wherein said step of actively regulating temperature comprises the step of maintaining sample temperature at less than about ambient temperature of said sample processing system.
5. A method of automated sample processing as described in claim 1 wherein said step of corresponding to at least one temperature tolerance.
16. A method of automated sample processing as described in-claim 13 wherein said step of actively regulating a temperature of a reagent comprises the step of maintaining reagent shelf life.
22. A method of automated sample processing as described in claim 20 wherein said step of regulating temperature with a Peltier grid comprises the step of regulating temperature with a plurality of thermal elements each corresponding to a sample Garner support.
27. A method of automated sample processing as described in claim 26 wherein said step of adaptively regulating temperature of said sample comprises the steps of determining at least one desired sample temperature; and decreasing temperature of said sample to said desired sample temperature.
28. A method of automated sample processing as described in claim 27 wherein said step of adaptively regulating temperature of said sample further comprises the steps of:
determining a sample temperature tolerance; and maintaining said sample temperature within said sample temperature tolerance.
44. A method of automated sample processing as described in claim 1 wherein said step of determining a processing sequence for at least one sample comprises the step of determining a microarray processing sequence.
48. An automated sample processing system comprising:
an automated sample processing system; and a Peltier device to which said sample is responsive.
50. A automated sample processing system as described in claim 48 wherein said Peltier device comprises:
a first heat sink a first fan configured to be interior of an interior volume of a sample processing system;
a second heat sink; and a second fan configured to be exterior of an interior volume of a sample processing system.
51. A automated sample processing system as described in claim 48 wherein said Peltier device comprises at least one thermoelectric device.
56. An automated sample processing system as described in claim 55 and further comprising a carrier support to which said at least one sample is responsive and wherein said Peltier device comprises at least one temperature regulation element configured to said sample Garner support.
59. A method of automated sample processing, comprising the steps of providing at least one sample;
providing at least one reagent;
regulating a temperature of said reagent; and automatically processing said sample.
64. An automated sample processing system comprising:
an automated sample processing system to which said sample is responsive; and a reagent temperature control element to which said reagent is responsive.
73. A method of automated sample processing comprising the steps of:
acquiring at least one temperature tolerance for at least one protocol;
transmitting said at least one temperature tolerance to at least one sample processing system;
adaptively processing at least one sample with said at least one sample processing system in accordance with said at least one temperature tolerance;
and acquiring sample processing information from said at least one sample processing system.
75. An automated sample processing system comprising:
an automated sample processing system to which said sample is responsive; and an active temperature regulation element to which said sample is responsive.
85. An automated sample processing system as described in claim 75, 78, 82, or wherein said active temperature regulation element to which said sample is responsive comprises a Peltier device.
86. An automated sample processing system as described in claim 75, 78, 82, or wherein said active temperature regulation element to which said sample is responsive comprises a Peltier grid.
87. An automated sample processing system as described in claim 75, 78, 82, or wherein said active temperature regulation element to which said sample is responsive comprises a conductive device.
88. An automated sample processing system as described in claim 75, 78, 82, or wherein said active temperature regulation element comprises a plurality of thermal elements each corresponding to a sample carrier support.
89. An automated sample processing system as described in claim 75, 78, 82, or wherein said sample comprises a batch of samples.
90. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated immunohistochemistry processing system.
91. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated in-situ hybridization processing system.
92. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated fluorescent in-situ hybridization processing system.
93. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated microarray processing system.
94. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated target retrieval processing system.
95. An automated sample processing system as described in claim 75, 78, 82, or wherein said automated sample processing system comprises an automated stainer processing system.
CA2508001A 2002-12-20 2003-12-22 Systems and methods of sample processing and temperature control Active CA2508001C (en)
US43560102P true 2002-12-20 2002-12-20
PCT/US2003/040880 WO2004059287A2 (en) 2002-12-20 2003-12-22 Systems and methods of sample processing and temperature control
CA2508001A1 true CA2508001A1 (en) 2004-07-15
CA2508001C CA2508001C (en) 2019-03-19
CA2508323A Active CA2508323C (en) 2002-12-20 2003-12-15 Method and apparatus for pretreatment of biological samples
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CA2974221A Pending CA2974221A1 (en) 2002-12-20 2003-12-19 Information notification sample processing system and methods of biological slide processing
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[Culture] Minahasa Traditional Weaving: Bentenan – 101MonkeyMagic
Traditional weaving in Minahasa tribe exists since the 7th century using threads made from wood fibre (Fuya) from Sawukouw and Lahendong areas, from pineapple fibre (Koffo), much like Filipino’s Piña fibre and from bamboo fibres (Wa’u).
Since the 15th Century though, the threads use cotton fibre. Some area still have the traditional Minahasa motifs Bentenan (name after the village on the Southeast coast of the Minahasa region where it comes from).
The Bentenan Centre in Sonder Village is handled by Karema (Kreasi Masyarakat Sulawesi Utara or People’s Creation of North Sulawesi), with the owner and head of the foundation Mrs. Onny Markadi. The guide for the day is Mrs. Nana.
The process is called Ngeteng or rolling the cotton threads and layout them in a wooden frame (Plangkan). The threads come from Java island.
The double tied (ikat)
Usually the process use 2 to 3 colours for a pattern
The dye basin using chemical colouring for brighter colours instead of tie-dye with natural colours
The threads are layout to create the traditional Bentenan pattern (merambang).
Each treads then spin in a spindle before weaving
An older version of the traditional weaving tool
A newer version traditional weaving machine ATBM (Alat Tenun Bukan Mesin)
The process take about 5 to 6 months from tie-dying to weaving. For each pattern run can made 30 to 60 pieces. Each finished product is around 115 cm to 260 cm long in various length.
There are 7 Bantenan patterns; Tonilama (weaving using only white threads), Kokera (flowers pattern weaving with beads), Pinatikan (hexagon shape in net pattern), Sinoi (stripes pattern), Tinonton Mate (humanoid pattern), Tinompak Kuda (repeating motifs), Kaiwu Patola (India pattern).
Most fabric sold in Karema is printed on silk, polyester, cotton, chiffon etc. They’re affordable for clothes, scarf or just for collection. There are selected weaving cloths, but they are usually expensive.
Manado: Jl. Achmad Yani no.8, Sario-Manado
Phone (+62) 431 862-193
Jakarta (Karema Galery) : Jl. Biduri Blok i 2/24, Permata Hijau-Jakarta Selatan
Phone (+62) 21 548-2765 ; opening hours 09.00 am-16.00pm
Kawanua Aerotel (North Sulawesi Representative)
Jl. Cempaka Putih Raya No. 120 Jakarta Pusat 10510
Ph: +6221 424 87 68 ; opening hours 09.00 am-16.00pm
Manado Trade Center (MTC) : Ground Floor no. H 54,56-53,55. Phone: (+62) 431-881 9355
Workshop (Bentenan Center):
Desa Kolongan Atas Dua, Sonder
Phone (0431) 354-464
CeritaKain, Culture, Fashion, Lifestyle
Indonesia, North Sulawesi, Sulawesi, Tomohon
2 thoughts on “[Culture] Minahasa Traditional Weaving: Bentenan”
[Culture] House on stilts in Woloan, Tomohon – 101MonkeyMagic

type: global

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text:
Leonardo DiCaprio | Orual Undone
Lessons from the Silver Screen: Inception (Mit Spoilers) July 28, 2010
Posted by orualundone in Art, Death, Lies, Lifescripts, Loss, Movies, Truth.
Tags: Christopher Nolan, dreams, Inception, Leonardo DiCaprio
Inception is one of the best movies I’ve seen this year (the very best being Toy Story 3). It is original in plot with superb story-telling, impressive acting and scripting, and stunning visual effects. Not to mention it’s quite a mind-bender. But it made me think about more than just where the story was headed or the implications of the final scene. As I have mentioned, I love to explore spiritual themes in movies and this movie had several that caught my attention. A discussion, rife with spoilers after the jump.
Please note, I am assuming that anyone reading this has seen the movie, so I am not going to provide a super-detailed synopsis. That would be an insult to Mr. Nolan’s creation, and, frankly, exhausting. I will just outline the basic plot elements relevant to what I’m talking about.


text:
homeownership | Vienna Orange Line
There’s A New Law In Town! How it Affects You!
Posted by leilacbrown in Agency Law, Buy A Home Now, Using a Broker
Agency law, homeownership, using a buyers agent
Are you a newbie? (never have purchased a home before), or maybe you’re like many folks who had a short sale a few years ago and are now ready to navigate the buying waters again. Or maybe you’re a renter. Congratulations! Let me be the first to inform you that in Virginia, there are new agency laws that became effective July 1, 2012. Perhaps you remember in the past, your agent may or may not have given you a buyer agency agreement or a renters agency agreement to read and sign. Well, now all real estate agents working with buyers and renters on the purchase or rental of a residential property, MUST have a written brokerage agreement in place from the outset of their relationship. Actually, written agency disclosure has been required for years and written buyer broker and rental broker agreements have been available and in use for over a decade. In the past, these agreements, if even used, may have been signed at the time an agent wrote an offer or when rental paperwork was due in the office. Now, an agreement MUST be in place before an agent shows you any property or begins to work with you. It doesn’t have to be a long term agreement. It might just be in effect for one day.
So when you call your agent to see a property, don’t be surprised when you are asked to formalize the arrangement by signing a disclosure form. Just be sure to read it carefully and make sure that it has a duration period that you have chosen. For more information visit the Northern Virginia Association of Realtors.
Let me know if I can help you or anyone you know with real estate purchasing or investing (residential or commercial) in the Washington DC area.
Would you Pay $1,980,000 to Live Near the Vienna Metro Orange Line?
Posted by leilacbrown in Buy A Home Now, Home Maintenance, Metro Stations, Uncategorized, Vienna-Fairfax Metro
fairfax homes, homeownership, housing upgrades, northern virginia market, Vienna Metro Station
[UPDATE] Owners Changed Realtors and the Price Has Been Reduced to $1,795,000. It is BACK ON THE MARKET!
Well, apparently one investor thought that was the right number!! Yes, indeed. An existing 1960’s brick home was demolished at 2926 Sayre Drive last year in the Poplar Terrace Neighborhood. In its place, in the middle of other remaining 1960’s homes, is a 6,764 square foot “mini mansion” with 6 bedrooms, five full baths and 2 half baths. It has a lot of upgrades: marble floors, hardwood, crystal chandeliers at every turn, a gourmet kitchen with granite counters and stainless steel appliances. The property has a 2 car garage and is gated. And you can walk to the metro station! The property was originally listed at $1,980,000 in December. It was reduced to $1,800,000. It is now temporarily off the market as of March 28 . Now, I’m not saying it won’t sell, but its going to take a special type of person with a special need or vision for this one. If it does sell, think of what it would do for other properties in the area.
What do you think??? Please let me know!
Live Near The Metro!
Posted by leilacbrown in Dunn Loring Metro, Homes Near Metro Stations, Metro Stations, New Townhomes, Real Estate Market, Real Estate Near the Orange Line Metro
Dunn Loring Metro Station, homeownership, investing in property, market update, Metro Stations, neighborhoods, northern virginia market, services of a buyers agent
I was in Mexico last week, but I understand there was a realtor luncheon at the brand new mixed-use neighborhood at the site of the old Merrifield Cinema (I spent many an afternoon and evening there over the years, ah but I digress). EYA is building 112 new townhomes there featuring three and four bedroom plans, garage parking and rooftop terraces. Three models are being sold with delivery as early as the summer of 2012.
If these homes are anything like those they are selling in Washington, DC, I think you will be pleasantly surprised. I’ve visited EYA’s community at Chancellor’s Row a few times and I found the models and layouts well thought out and convenient for younger singles and couples. . . . there are a lot of stairs!
Of course, the Mosaic District (as it is called) will have restaurants, boutique retail stores, a theater and more for all the new residents and those of us who live nearby. If you are interested in seeing these new townhomes, why not give me a call? Remember that the sales representatives are working for the builder. You need your own representation. It costs you nothing and it could save you a bundle! leila@mris.com or 703 679-8741.
Realty Times – My Home For Sale: A Behind-the-Scenes Look at the Home-Selling Process
Posted by leilacbrown in Information for Homeowners, Real Estate Market Update, Remodeling, Renovations
and maintenance issues, fairfax homes, homeownership, housing upgrades, selling your home
Realty Times – My Home For Sale: A Behind-the-Scenes Look at the Home-Selling Process.
Washington DC Housing Market Remains Strong
Posted by leilacbrown in Buy A Home Now, Real Estate Market, Real Estate Market Update, Uncategorized
homeownership, real estate update, washington dc market, washington real estate
Report: D.C. housing still leads nation
Washington Business Journal by Jeff Clabaugh, Broadcast/Web Reporter
Date: Thursday, December 8, 2011, 10:16am EST
Jeff Clabaugh Broadcast/Web Reporter – Washington Business Journal Email Another report on housing prices affirms the Washington, D.C., housing market remains among the healthiest. Truckee, Calif.-based real estate data firm…
Posted by leilacbrown in Buy A Home Now, Buying vs. Renting, Real Estate Market, Wall Street Journal
buying vs. renting, homeownership, real estate rents, real estate update, rental market, wall street journal
<a href=”http://goo.gl/vqWr3/”See complete article here”
Home Prices Headed Higher?
Posted by leilacbrown in First Time Home Buyers, Mortgage Information, Real Estate Market, Real Estate Market Update
first time homebuyers, homeownership, national real estate news, real estate market, real estate update, virginia market update
from Darius Jenkins, McLean Mortgage Corporation, Cell: (703) 628-5513 | djenkins@mcleanlending.com, www.loansbydarius.com
For the entire story >>http://www.mortgageminute.info/
President Obama signs HR 2112, extends FHA and VA loan limits
Posted by leilacbrown in Fannie Mae, FHA, First Time Home Buyers, For Sale By Owner, Information for Homeowners, Mortgage Information, Real Estate Market
FHA Loans, homeownership, market update, mortgage rates, national real estate news, VA Loans, virginia market update
November 21, 2011 | by Anne
Original article from AGBeat.com.
Loan limit increase finalized
We reported last week that bipartisan Congressional efforts passed HR 2112, the Consolidated and Further Continuing Appropriations Act of 2012 to restore higher FHA loan limits which expired on September 30, 2011.
This agreement (that came with riders) increased the maximum dollar amount of mortgage loans that can be insured by the Federal Housing Administration (FHA) back to $729,750 after dropping the cap to $625,500 automatically after a temporary increase was issued for all loans insured by the FHA. The restored higher limit will remain in place through 2013 but does not include Fannie Mae or Freddie Mac backed loans.
Representatives Gary Miller and Brad Sherman (R-CA) successfully got a measure passed that created a conforming loan limit cap of $625,000 in areas with median home prices over $417,000 before it was initially increased to $729,750 which expired and was passed by a 298-121 vote in the U.S. House of Representatives and a 70-30 vote in the Senate.
Obama signs HR 2112 into law
HR 2112 has passed and President Obama signed into law HR 2112 late on Friday. The bill provides $128 billion in discretionary appropriations to provide funding for three 2012 appropriations bills (Agriculture, Transportation-Housing and Urban Development and Commerce-Justice Science).
The Consolidated and Further Continuing Appropriations Act of 2012 provides $1.3 billion to HUD for administration, up $16 million from 2011 but down $18 million from the President’s request.
VA Loan Information (from Veterans Administration Website) (11-29-2011)
[11/22/2011 – Latest Funding fee Update!! On November 21, 2011, President Obama signed HR 674, Vow to Hire Heroes Act of 2011 that includes a provision to revert funding fees to their former higher level through September 30, 2016. We have released circular 26-11-19, which provides more information. Please monitor this site and the Funding Fee Payment System site for information.]
Recent Home Sales Near the Vienna Metro
Posted by leilacbrown in First Time Home Buyers, Information for Homeowners, Real Estate Market, Real Estate Market Update, Townhomes, Vienna-Fairfax Metro
Bus Service to NYC from Vienna Metro, Condos, homeownership, homes around the vienna metro, housing market, market update, monthly real estate update, vienna orange line
These homes were sold between September 1, 2011 and November 18, 2011
Address9480 Virginia Center Blvd #433 Close Price$205,000 Advertised SubdivisionAcadia
9718 Kingsbridge Dr. #303 $181,000 HawthorneVillage
3115 Buccaneer Ct. #201 $199,900 HawthorneVillage
9480 Virginia Center Blvd #333 $210,000 Acadia
2765 Centerboro Dr. #261 $214,000 Marquis atViennaMetro
2791 Centerboro Dr. #187 $300,000 Marquis atViennaMetro
9480 Virginia Center Blvd #335 $335,000 Acadia
9480 Virginia Center Blvd# $335,000 Acadia
9625 Nonquitt Dr. $330,000 Tudor Hall
3074 Sugar Lane $332,500 CyrandallValley
9480 Virginia Center Blvd #439 $300,000 Acadia
9633 Lindenbrook St. $350,000 Linden Square
9648 Lindenbrook St. $341,000 Linden Square
2935 Everleigh Way $355,000 Covington
9502 Poplar Leaf Ct. $365,000 Circle Woods
2931 Deer Hollow Way #307 $390,000 Hunter Branch
3082 SouthernElm Ct. $391,750 Circle Woods
9878 Oakdale Woods Ct. $360,000 Oakton Cloisters
9115 Vosger Ct. $392,000 Hampton Court
9914 Brightlea Dr. $415,000 Edgelea Woods
2932 Waterford Ct. $400,000 Waterford
1112 Pekay St. SW $385,000 ViennaWoods
9657 Masterworks Dr. $462,500 Country Creek
1115 Drake St. $475,000 ViennaWoods
9731 Water Oak Dr. $495,000 Five Oaks Place
2918 Rensselaer Ct. $520,000 Ivy Square
806 Tapawingo Rd. $530,000 Townes of Moorefield
534 Marshall Rd. $548,500 ViennaWoods
9638 Black Maple Dr. $560,000 Beech Grove
3141 Blue Barn Way $570,000 Beech Grove
636 Marshall Rd. $570,000 Moidone Acres
2880 Crossbow Ct. $615,000 Edgelea Woods
2969 Covington SquareWay $612,000 Towns ofCovington
9419 Van Arsdale Dr. $620,000 VirginiaCenter
9951 Leamoore Lane $730,000 Edgemoore
Not Every Real Estate Market Is Struggling – Bloomberg BusinessWeek
Posted by leilacbrown in Metro Stations, Real Estate Market, Real Estate Market Update, Vienna-Fairfax Metro
foreclosures, homeownership, market update, northern virginia market, Vienna Metro Station
In the Obama years, home values lagged throughout the U.S., with a few exceptions
The Obama Administration, the inheritor of a collapsed housing market and financial crisis, has tried to help the hardest-hit housing markets through refinancing and loan modifications. Yet individual markets are influenced heavily by local conditions, such as jobs, school districts, and population growth. Both the best-performing market, Weston, and the worst, Homestead, are in the Miami-Fort Lauderdale metro area, for instance. As housing trends at their core are hyperlocal, it makes it difficult to even craft regional solutions. Developing a catch-all national policy is even more challenging.
Wong is a lifestyle and real estate reporter for Bloomberg Businessweek.


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5 Reasons why TINYS is good for your mental health – Tanya Tamrakar
5 Reasons why TINYS is good for your mental health
Posted on July 23, 2017 by Tanya Tamrakar in Books
Savi Sharma is the India’s fastest rising author in the self-publishing era. A storyteller who flushed youth’s mind with the aura called – SUCCESS. Savi Sharma debuted with ‘Everyone Has A Story’ which became best-seller; which was fore taken by Westland Ltd and second book ‘This Is Not Your Story’. Savi Sharma, a CA aspirant who quit Final examinations to tell stories to the audience. One of them which has been discussed above.
REASONS WHY YOU MUST READ ‘THIS IS NOT YOUR STORY’
1. BRAIN WASHES MIND TO STEP AHEAD
Savi literary flushes your mind and fills with positivity and the ray of hopes. One cannot simply deny the fact that TINYS doesn’t hit your mind. A dope of possibilities, hopes and strength is directly injected to your mind thoroughly. The character, Shaurya quits his CA to chase after his own dream (and not parents) of film-making. Shaurya, who lacked the courage to tell his parents about the dream since he was bullied at the thought of film-making when he first discussed with it them. Of how he pedals his bi-cycle to the tracks, must be read.
2. RESTORES FAITH IN YOURSELF
One cannot simply move ahead if they are lacking in faith in themselves. Unless you are sure about what, how and when to be in the right track. It gives us a shot of how to trigger your gun. To start all over again and move ahead of all odds. Shaurya, Miraya and Anubhav set best examples of the same.
3. DISCUSSED THROUGH EACH ASPECT
Not through the individuals; love, friendship and family plays an important role in one’s life. Thus, every topic is discussed thoroughly and how these three relationships affects an individual’s decision. Apart from fantasies, TINYS focuses on real lives as well. How Shaurya is brought up tactically with his friends and how they play an important role in his life.
4. DIRECTS INSPIRING OF LIFE IS ALL ABOUT SECOND CHANCE
Sometimes individual fells deep and deep that they forget to rise again. But as we say, every story deserves a part two. Savi explained that beautifully, when everything falls off shore, how to take steps at that point of time. Sometimes, everything you need to overcome is to meet; from the one you are hiding from. To make things happen again, you need to be in the right place; of sure.
5. KEEPS YOU FEVI-KWIKED
Story doesn’t let you stop reading, rather it keeps you awake. Book will always keep you hooked up – from morning till the mid-night. The book will surely be your bed partner for several days. You cannot stopover the book, neither skip any part. Every part holds anew story, especially Miraya’s diary keeps you stick with herself. Containing around 200 pages, the book is not something you cannot read it, it’s time friendly and after all, motivating.
Savi Sharma has literary taken world by storm, it can be proved by ‘Everyone One Has A Story’ and ‘This Is Not Your Story’, of which both of her stories were a blockbuster and be read for sure.
Tagged #everyonehasastory, #motivation, #savi, #savisharma, #selfpublishedwriter, #storyteller, #thisisnotyourstory, #youngwriter
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One thought on “5 Reasons why TINYS is good for your mental health”
Dodie Rabena says:
Constantly a pleasure to review your content, seems you really do have a skill for producing great web content!
Remember? I always speak with two meanings in one sentence. My another moon must be reading this! __ #moonquotes #darkness #rayofhope #relatable #tanyatamrakar #innerme #


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Laura's House of Halloween Blog: Top 13 Halloween Party Songs
Well, it's finally time for another top 13 list! I'm pleased to bring you my choices for the Top 13 Halloween Party Songs. These are basically upbeat songs that have either a Halloween theme or some kind of horror theme to them. So, let's get started!
#13 I Put A Spell On You - Bette Midler
I admit, I prefer Screamin' Jay Hawkins version of this song, but Bette Midler's is much more upbeat and would be funner in a party atmosphere. It's a short song but has great energy and is worthy of any Halloween party playlist.
#12 Pet Sematary - The Ramones
Quite possibly the most mismatched theme music to a movie (tempo wise, not lyric wise) in history, this song is catchy as hell and has a great beat. Even if you haven't seen the movie or read the book, the song stands on its own well.
#11 Halloween - Aqua
This song is a little obscure, but is the one with the most traditional "party" sound on this list. I love that it pays homage to old slasher movies.
#10 Ghostbusters - Ray Parker Jr.
As a child of the 80s, I had to include Ghostbusters! This song just makes me smile every time I hear it.
#9 Werewolves of London - Warren Zevon
In my opinion, this is a classic Halloween song. Good, silly fun!
#8 (I Always Feel Like) Somebody's Watching Me - Rockwell
With backing vocals by Michael Jackson, who doesn't love this song? Great 80s nostalgia, creepy yet comical, catchy beat...this is one of my faves!
#7 Bad Moon Rising - Creedence Clearwater Revival
There's a bad moon rising...let's dance!
#6 This Is Halloween (from The Nightmare Before Christmas)
Whether you prefer the original or Marilyn Manson's version, this song is a must-play at any Halloween party!
#5 Time Warp (from The Rocky Horror Picture Show)
Rocky Horror has become a staple of Halloween, and Time Warp is arguably the most popular song from it...with good reason! It's a great song that's easy and fun to dance to. It's just a jump to the left...
#4 Halloween - The Misfits
Although perhaps not the most mainstream of songs (or bands), the Misfits' "Halloween" brings a different flavor to any Halloween party. If you're looking for music that is a little less "kid friendly", the Misfits has a great library of songs. I particularly like "Halloween" because it touches upon ancient rituals of the Druids and more of the "darker side" of the holiday.
#3 Don't Fear The Reaper - Blue Oyster Cult
A Halloween staple, pure and simple. Not much more I can say than that.
Even people who don't care for Michael Jackson, still seem to love Thriller. Thriller is one of the funnest songs to dance to, even if you don't know the official dance moves.
#1 Monster Mash - Bobby Pickett
You probably saw this one coming. Monster Mash might just be the most popular song associated with Halloween. It's fun, it's upbeat, and pays homage to classic horror monsters. What could be better?
So, that wraps it up! Do you agree with this list? What songs would you play at a Halloween party?
Labels: Halloween Songs, Top 13 Lists

value: Blogger is an American online content management system (CMS) which enables multi-user blogs with time-stamped entries. Pyra Labs developed it before being acquired by Google in 2003.


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Then Play Long is No More | songs from so deep
← Holiday Harmonies Part 7: My Old School – Steely Dan Their Back Pages →


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The Square Metre at TQ 78286 18846: April 2011
Birch comes of age
The birch tree that appeared from seed 7 years ago in spring 2004 in the south west corner of the Square Metre itself, has a few green catkins among its delicate leaves.
I estimate the tree is now about 6 metres tall.
These are the first catkins that have been produced and it shows how quickly birches can reach reproductive size from seed.
There is much activity in Emthree in the welcome warm weather. Many of the usual insect friends are making an appearance and there seem to be more solitary bees this year paying particular attention to North Wall which is becoming more earthy as it collapses into the mould.
Cardinal beetles are on the wing and this red-headed (Pyrochroa serraticornis) is resting in the shade under a bramble leaf.
The wild rose that appeared in the square metre itself in spring 2004 and which was later moved to the north west corner of the square is growing well this year, though still a long way from flowering. On one of the leaflets I found a tiny moth caterpillar chomping little holes out from beneath a thin veil of silk.
Artist's conk fungus on the medlar
At the base of the medlar trunk I found a, small, hard bracket fungus. With the help of Martin Allison, the county fungus recorder, this was determined as Ganoderma applanatum, the artist's bracket or artist's conk.
A distinguishing feature is the soft, thin, whitish surface underneath which if scratched leaves a brown mark. This surface can be used for writing or drawing as with white scraperboard (or scratchboard). The example below is on the under side of the fungus above. It looks strangely like a graffito written over a cave painting.
Some artists have produced remarkable effects. Have a look at these drawings by Marie Heerkens which are somewhat better than my script:
http://goo.gl/8imxb
Recent research has shown that chemicals from this fungus could be of value in the treatment of diabetic complications. It is also said to make an excellent, if rather bitter, medicinal tea. However, what it was excellent for (or against) was not specified. It can also be used for dyeing wool, some fabrics, or paper and will yield a rust colour with wool when ammonia is used as a mordant.
Ganoderma applanatum causes white rot within the host tree, which explains why a large chunk of the medlar died back last year. I expect the rest will go fairly quickly, but some suckers may survive. It is rather sad really as it is a wild medlar (with thorns) that came, many years ago, from the bank close to my parents house at top of Whydown Hill at the far south of the parish. Ganoderma, mankind or medlar, we are all headed for the same destination.
Forget-me-not time again
The forget-me-nots (Myosotis sylvestris) are in flower now, little sky mimicking flowers between Midsummer Pond and the western edge of Emthree.
There is a certain vagueness about this plant, well expressed by John Hill who wrote in 1756 "It is said to be an astringent, but its virtues are not certainly known."
People used to give someone forget-me-nots if they were going on a journey on 29 February. Since it is not usually in flower at that season of the year, this could be problematical.
Writers like it. For example Miss Price in Dylan Thomas's Under Milk Wood said "I will knit you a wallet of forget-me-not blue, for the money, to be comfy."
John Neal in Goody Gracious and the forget-me-not wrote "if you get tired of being here, all you have to do will be just to pull it up out of the earth, and wish yourself at home, and you will find yourself there in a moment, in your own little bed." I wonder what he was on.


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Black Women, Stacy English parents push for speedier missing persons reports | Souls of Black Women ~ Souls of Black Women
Black Women, Stacy English parents push for speedier missing persons reports | Souls of Black Women
The parents of the deceased victim, Stacy English are pushing for missing persons reports to no longer be a lengthy amount of time. We do not know if you recall who the Atlanta Woman is, but Stacy English is the black woman who went missing and most people thought it was her ex-boyfriend who had allegedly done harm to her. However, after her car was mysteriously found abandoned with the engine still running in a towing car lot, it took some time for police to find her. Her body was discovered in a woodsy area underneath a fallen tree...
Posted in: atoast2wealth.com, black woman hair, black women stereotypes, buckhead woman's parents new bill for missing persons, soulsofblackwomen stacy english, stacy english push for speedier missing persons reports


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Internet Archive Search: subject:"Psychiatric hospital patients"
montana state library. patients' library
by Wallace, Wendy, 1956-
Outside London behind a stone wall stands Lake House, a private asylum for genteel women of a delicate nature. In the winter of 1859, recently-married Anna Palmer becomes its newest arrival, tricked by her husband into leaving her home, incarcerated against her will and declared hysterical and unhinged. With no doubts as to her sanity, Anna is convinced that she will be released as soon as she can tell her story. But Anna quickly learns that liberty will not come easily. And the longer she...
Topic: Psychiatric hospital patients
Vol 1994: Annual report
by Montana State Library. Patients' Library; Montana State Library Commission; Montana State Hospital
Topics: Psychiatric hospital patients, Hospital libraries
Vol 1991: Annual report
Vol 1992: Annual report
Vol 1: Index of ... post-mortems of the insane, State Hospital for the Insane, Norristown, Pa.
by State Hospital for the Insane for the South-eastern District of Pennsylvania.
Topic: Psychiatric hospital patients Autopsy Pennsylvania.
by Neuman, Fredric
Topics: Psychiatric hospital patients, Psychiatric hospitals
Vol 1993: Annual report
Vol 1989: Annual report
Vol 1990: Annual report
Vol 2: Index of ... post-mortems of the insane, State Hospital for the Insane, Norristown, Pa.
by Turrill, David A
Topics: Psychiatric hospital patients, Abused wives, Widowers, Abused wives, Psychiatric hospital patients,...
Use of seclusion and restraints in mental hospitals : hearing before the Committee on Finance, United States Senate, One Hundred Sixth Congress, first session, October 26, 1999
In folder: Public laws / Hearings / Senate / 106th Congress / 1st session / 1999
Topics: Psychiatric hospital patients, Psychiatric hospital patients, Mental health laws, Psychiatric...
A bill to protect the rights of residents of certain federally funded hospitals
by United States. Congress. Senate; United States. Congress. Senate. Committee on Health, Education, Labor, and Pensions
CMS copy in folder: Public Laws: Bill / Senate / 106th Congress / 1st Session / 1999 / File 3
Topics: Psychiatric hospital patients, Psychiatric hospital patients, Federal aid to hospitals, Sentinel...
by Claremont, Máire, author
"Signet eclipse historical romance"--Spine
Topics: Asylums, Psychiatric hospital patients, Social conditions, Psychiatric hospital patients, Social...
by Gray, T. M
Topics: Psychiatric hospital patients, Haunted houses, Summer resorts
Reprint of the 1964 ed. published by Viking Press, New York, in series: A Viking compass book
Topics: Psychiatric hospital patients, Psychiatric nurses, Mentally ill
by Meganck, Glenn, 1955-
Topics: Psychiatric hospital patients, Auditory hallucinations, Angels
Mother London : a novel
by Moorcock, Michael, 1939-
Topics: Psychiatric hospital patients, World War, 1939-1945
Topics: Psychiatric hospital patients, Mentally ill women, Solitude
by Percy, Walker, 1916-1990
Topics: Men, Depressed persons, Schizophrenics, Psychiatric hospital patients
by Slaughter, Frank G. (Frank Gill), 1908-2001
Topics: Surgeons, Mental illness, Psychiatric hospital patients
by Becker, Tom, 1981-
Topics: Mothers and sons, Psychiatric hospital patients
Ge li dao = Shutter island
Topics: United States marshals -- Fiction, Psychiatric hospital patients -- Fiction, Brainwashing --...
by Roy, Jacqueline, 1954-
Topics: Female friendship -- Fiction, Jamaicans -- England -- Fiction, Psychiatric hospital patients --...
by Walker, Sue, 1960-
Originally published: London : Michael Joseph, 2004
Topics: Psychiatric hospital patients, Ex-mental patients, Problem youth, Sudden death, Ex-mental patients,...
The last Yankee : with a new essay about theatre language
by Miller, Arthur, 1915-2005
Topics: Psychiatric hospital patients -- Drama, Mentally ill women -- Drama, Marriage -- Drama, Marriage,...
Ekaterina : a novel
by Harington, Donald
"Ekaterina you were, and you were not at all. You were from a land far away, once upon a time and upon no time at all..." So begins our friendly narrator, who happens to be dead. Ekaterina has just arrived in an unnamed city at the confluence of the Allegheny and Monongahela with a pasteboard suitcase, a kerchief that covers her lack of hair, and little more than a rudimentary knowledge of English, the language in which she will eventually write Georgie Boy and her other phenomenal...
Topics: Psychiatric hospital patients, Teacher-student relationships, Women authors, Pedophilia
Teatro y comunidad terapéutica
by Castro-Pozo, Tristán
114 p. : 24 cm
Topics: Psychodrama, Psychiatric hospital patients, Psychiatric hospitals -- Sociological aspects
by McCauley, Sue
Topics: Psychiatric hospital patients, Mentally ill women, Married women, Youth, Maori
Tuscaloosa : a novel
by Phillips, W. Glasgow, 1969-
Topics: Psychiatric hospital patients, Psychiatric hospitals, Fathers and sons, Young men
by Ludlum, Robert, 1927-2001
Originally published: New York : St. Martin's, 2005
Topics: Psychiatric hospital patients, Intelligence officers, Identity (Psychology), Escapes
Failure to zigzag : a novel
by Vandenburgh, Jane
Topics: Teenage girls, Grandparents, Mentally ill women, Psychiatric hospital patients
by Lehrer, James
Confined to a Missouri state insane asylum, a boy named Birdie is taken under the wing of Josh, an elderly patient who has learned the ropes at the institution and who eventually helps him escape, only to return himself to the asylum
Topics: Psychiatric hospital patients, Homeless persons, Male friendship, Older men, Boys
The treatment of the insane without mechanical restraints
by Conolly, John, 1794-1866; Middlesex Lunatic Asylum at Hanwell
Includes extracts from the annual reports of Hanwell asylum, 1839-1849: p. 192-273
Topics: Mentally ill, Psychiatric hospital patients, Mentally Ill Persons
by Dudman, Clare
Includes bibliographical references (p. 338-340)
Topics: Psychiatric hospital patients, Mentally ill women, Jewish women
by Moore, Marshall, 1970-
Topics: Gay men, Psychiatric hospital patients, Murder victims' families
by Ludlum, Robert, 1927-
by Plath, Sylvia, 1932-1963
Topics: Psychiatric hospital patients, Women periodical editors, Mentally ill
Bird-eyes
by Arnold, Madelyn
Topics: Lesbian teenagers, Psychiatric hospital patients, Female friendship, Deaf women
Topics: Psychiatric hospital patients -- Fiction., Psychiatric nurses -- Fiction., Mentally ill -- Fiction.
Topics: Psychiatric hospital patients, Ex-mental patients, Problem youth, Sudden death
Topics: Fiction, Plantation owners' spouses, Psychiatric hospital patients, Asylums, History
Bolt risk : a novel
by Wood, Ann, 1970-
Topics: Stripteasers, Drug traffic, Drug addicts, Musicians' spouses, Psychiatric hospital patients
Genius of the sea
by Murr, Naeem
Topics: Merchant mariners, Psychiatric hospital patients, Mothers and sons, Suicide victims
Topics: Psychiatric hospital patients, Brainwashing, Drug testing, Eugenics, Islands
The patient : Wayne County General Hospital and Infirmary, Eloise, Michigan
by Wayne County Consultation Center Outpatient Department
Topics: Wayne County General Hospital, Psychiatric hospital patients, Hospitals, genealogy
Shooting the heart
by Cody, Paul, 1953-
Topics: Psychiatric hospital patients, Missing persons, Married people, Amnesia
by Elbom, Gilad, 1968-
Topics: Psychiatric hospitals, Psychiatric hospital patients, Young men, Nurses

value: An archive is an accumulation of historical records – in any media – or the physical facility in which they are located. Archives contain primary source documents that have accumulated over the course of an individual or organization's lifetime, and are kept to show the function of that person or organization.


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T-J Model Trains and Things (10,928)
Toy Soldiers ho 1/72 1/32 1/35 1/76 (7,240)
Shaffer's Train Shed (4,957)
K4 Supply Company (4,905)
184,504 listings
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Was: C $9,770.62
NEW Tenshodo 12102 EF30 1order Sealed beam Late type No.17 1/80 16.5mm Brass 482
UP/SP/C&NW BRASS FORTY-NINER 8 CAR SET by CHALLENGER made by samhongsa
C $4,950.38
BRASS AMTRAK / CALTRANS CALIFORNIA CARS 5 CAR COMPLETE TRAIN EXCELLENT COND.
C $4,885.25
HO Brass Rock Island "Rocky Mountain Rocket" 8-Car Set PSC #17980.1 MINT
TRAINBUILDER AUSTRALIAN VR S CLASS STREAMLINER WITH SPIRIT OF PROGRESS SET RARE!
Tenshodo Crown Japan JNR D52 468 Umekoji Perserved Version DCC Sound SUPERB
+C $45.60 shipping	Tenshodo Crown Japan JNR C62 25 Royal Trip Steam Engine Special Livery ULTRA RAR
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BRASS PSC 17980-1 CRI&P 1939 ROCKY MOUNTAIN ROCKET WESTBOUND 8-CAR SET F/P NEW
C $4,233.87
Brass MODELS Imon Chinese Railway Type-22 5 Coaches set Rare plus one Bachmann
+C $65.14 shipping	From Hong Kong
Tenshodo Crown Japan JNR C57 1 Yamaguchi Perserved Version Steam Engine SUPERB
+C $45.60 shipping	BRASS W&R NP 4-8-4 CLASS A-2 #2650 F/P PASSENGER GRAY
Was: C $4,559.61
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+C $89.88 shipping	From Italy
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value: eBay Inc. ( EE-bay) is an American multinational e-commerce corporation based in San Jose, California, that facilitates consumer-to-consumer and business-to-consumer sales through its website.


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Awkward press conference for Palin « ||BanglaClub||.your place to be
Two hours before Thursday morning’s press conference at the Republican Governors Association — her first since the Republican presidential ticket lost last week — Alaska Gov. Sarah Palin was still scheduled to appear alone. Instead, she spoke with a row of fellow governors standing silently behind her.
Mississippi Gov. Haley Barbour told CNN producer Evan Glass that they all met at 9 a.m. — an hour-and-a-half before the press conference’s scheduled start time — and by then it had been “decided” that they’d all go out together.
An RGA official told CNN the reason for the change is a “long story.”
He said that when the governors were all at their private morning meeting, someone brought up the desire to get beyond what happened in the McCain campaign and look towards 2009 and 2010.
Then, this source said, Palin piped up and said she agreed that she didn’t want to talk about the past.
This source insists that it was then decided that the other governors in the meeting would go with her to her press conference as a “show of unity.”
The source admitted that it may not have been easy for some “big egos” to go in and stand behind her, but they knew they’d be doing so.
Not present: the conference host, Florida Gov. Charlie Crist. A Florida GOP source tells CNN “he didn’t know about it,” because he wasn’t at the morning meeting.
In another shift, Palin — who had been slated to take questions for 20 minutes or so — took just four press queries.
Why did Texas Gov. Rick Perry cut it off so fast?
“We were running behind schedule,” insisted the GOP official.
Palin may not have wanted to talk about the past, but her speech was almost entirely about the McCain campaign; she included little in the way of detailed ideas about the way forward for Republicans, the theme of the panel.
November 13, 2008 - Posted by	Admin | News, Politics	| palin, sarah


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Wordpress single product työt ja työpaikat | Freelancer
Wordpress single product työt
157,040 wordpress single product työtä löytyi, hinnoittelu EUR
...the uploaded file I want the name underneath the logo to say Crown Jewels instead of Queen Adventures. Also instead of Serving Up Inspiration, Empowerment and Adventure For Single Women, I want that removed and there I want it to say: Inspirational Jewels Of Wisdom For Women. Lastly, where is has the website [kirjaudu nähdäksesi URL:n] .com I want that removed
...[kirjaudu nähdäksesi URL:n] 6)	[kirjaudu nähdäksesi URL:n] in this and all side menu options we need a pagination and load only 20 profile in a single page 7)	[kirjaudu nähdäksesi URL:n] i want a option for contact number (show to all paid members),(show to only members I accept/sent interest) 8)	We need to give
I need a Wordpress MULTISITE Single Sign-on (SSO) Plugin. I do have several domains like [kirjaudu nähdäksesi URL:n] & [kirjaudu nähdäksesi URL:n] etc.. All theses need to have synchronized login cookies. So... The Single Sign-on feature i need, synchronize login cookies on all the domains in whole multisite network. When ever a user login anywhere on ...
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value: Freelance (sometimes spelled free-lance or free lance), freelancer, or freelance worker, are terms commonly used for a person who is self-employed and not necessarily committed to a particular employer long-term. Freelance workers are sometimes represented by a company or a temporary agency that resells freelance labor to clients; others work independently or use professional associations or websites to get work.