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The Sector Most at Risk If U.K. Secedes? Finance, UBS Says - Bloomberg
Bloomberg Anywhere Remote LoginDownload SoftwareService Center MENU Homepage Markets Stocks Currencies Commodities Rates + Bonds Economics Magazine Benchmark Watchlist Economic Calendar Tech U.S. Global Startups Cybersecurity Digital Media Bloomberg Technology TV Hello World Studio 1.0 Pursuits Cars & Bikes Style & Grooming Spend Watches & Gadgets Food & Drinks Travel Real Estate Art & Design Politics Global Risk Briefing How We’ll Know if Trump Is Making America Great Again Tracking Trump’s Web of Conflicts The Inauguration of Donald J. Trump On the March in Trump’s Capital Opinion View Gadfly Businessweek Subscribe Cover Stories Opening Remarks Etc Features 85th Anniversary Issue Behind The Cover More Science + Energy Graphics Game Plan Small Business Personal Finance Inspire GO The David Rubenstein Show Sponsored Content Bloomberg About The Company Sign In Sign In Subscribe The Sector Most at Risk If U.K. Secedes? Finance, UBS Says
April 18, 2016, 5:56 AM EDT
Retail companies could also suffer if consumer sentiment sours
Energy, materials, beverages and tobacco stocks may benefit
Financial firms in the FTSE 100 have seen a stellar rebound since the U.K. market’s low two months ago. But to UBS Group AG’s wealth-management unit, they’re the ones that stand to lose the most if the nation leaves the European Union.Secession by Britain would hurt both national and international operations of financial institutions: those most tied to the domestic economy would suffer because leaving the EU could hamper the economy, while any change in the U.K.’s credit rating would push up funding expenses. Larger banks would be hit by an increase in costs arising from the greater risk attached to their British assets, and potentially more onerous rules for providing services in the EU, according to UBS.“The domestic banks are very linked to the U.K. economy as it is all about consumer lending and business lending,” said Caroline Simmons, the deputy head of U.K. Investment at UBS Wealth Management in London, without mentioning any specific stocks. The unit has about 31.7 billion pounds ($45 billion) of assets under management. “They would be more heavily impacted than international banks due to their larger exposure to the U.K. property market and to business capex spending. But if we see widening credit spreads in the U.K., it would be bad for all banks.”
Even as polls signal the June 23 referendum is too close to call, U.K. stocks have maintained the best performance among major western-European markets in 2016, and last week entered positive territory for the year thanks to a weakening pound and the rebound in energy and commodity producers. British lenders, in contrast, are still down about 15 percent this year, amid volatility almost twice that of the broader market, on concern that central-bank stimulus will hurt profits and low interest rates will squeeze margins.UBS Global Research is not the only one warning of the risks associated with financial stocks -- Morgan Stanley is recommending an underweight position due to the potential fallout of Britain leaving the EU. In a report dated April 6, it warned that Brexit concerns will “likely” intensify through the second quarter.On the flip side, some companies could prosper from a U.K. exit. Energy and commodity producers stand to extend gains, along with beverage and tobacco companies, according to Simmons, who cited the benefits of a weaker pound and their lower exposure to the domestic economy. Companies that generate the most revenue in the U.S. would suffer the least as they would remain relatively insulated from any economic slowdown in the U.K., Simmons said.If U.K. voters choose to stay in the EU, the a research report by UBS advises buying small caps and domestically focused companies including housebuilders, retail, and real estate, as well as those stocks that declined on concerns of an exit. In the event of a vote in favor of leaving, it recommends large-cap internationals.
Losers in case of Brexit (according to UBS Global Research): * Banks: Lloyds Banking Group Plc, Royal Bank of Scotland Group Plc and Barclays Plc may face higher costs and tighter credit conditions. * Retail-related companies: J Sainsbury Plc, Debenhams Plc and Restaurant Group Plc could lose as economic uncertainty damages consumer sentiment. Winners in case of Brexit (according to UBS Global Research): * Tobacco and beverage companies: Imperial Brands Plc and Diageo Plc could benefit from a weaker pound and their limited exposure to the U.K. economy. *Telecom companies: Vodafone Group Plc and Inmarsat Plc may gain from a weaker currency as much of their earnings are denominated in euros and dollars, respectively. Before it's here, it's on the Bloomberg Terminal.
Pound Whipsawed by Oil, Stocks; Osborne Warns on Brexit Cost


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ANCIENT GREEK HELLENISTIC POTTERY KANTHAROS 3rd CENT | eBay Skip to main content eBay Shop by category Enter your search keyword All Categories Advanced or register':' style="margin-right:3px">Hello -User-.'.replace(/-User-/,escape(a)))+"")}catch(b){document.write("")}})();Hello (Sign in to bid or buy)Daily DealsCollections My eBayExpand My eBay Summary Bids/Offers Watch List Wish List All Lists Purchase History Selling My Collections Followed Searches Messages Collect & Spend Learn more SellExpand Sell Sell an item Seller centre International selling Seller protection Seller Fees Sell for Charity CommunityExpand Community Answer Centre Discussion Boards Groups News Help & ContactExpand Help & Contact Contact Us (Phone / Chat / E-mail) FAQs Delivery & Returns Ask the eBay Community Resolution Centre Back to home page
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Greek Kantharos dated to the mid 3rd century.
The 10 cm high Kantharos has two high loop handles, with the body decorated in original brown pigments sme of which haqs rubbed
one restored handle Sold genuine as described and comes complete with a certificate of authenticity.

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Lives of the saints : from Mary and St. Francis of Assisi to John XXIII and Mother Teresa (Book, 2001) [WorldCat.org]
http://www.worldcat.org/oclc/45248363
Title: Lives of the saints : from Mary and St. Francis of Assisi to John XXIII and Mother Teresa
Author: Richard P McBrien
Publisher: [San Francisco] : HarperSanFrancisco, ©2001.
ISBN/ISSN: 006065340X 9780060653408 0060653418 9780060653415
OCLC:45248363
Presents a modern perspective on the lives of the saints, chronicling the histories and significance of individuals representing the best in Christian discipleship, and examines the meaning of sanctity, spirituality, and canonization.
BiographyReference sources
006065340X 9780060653408 0060653418 9780060653415
45248363
xxiii, 646 pages : illustrations ; 25 cm
Part I. Who is a saint? --
Part II. Saints and spiritualities --
Part III. Canonization: Process and politics --
Part IV. Lives of the saints --
Part V. Epilogue: Saints and the church --
Part VI. Tables.
Richard P. McBrien.
Add tags for "Lives of the saints : from Mary and St. Francis of Assisi to John XXIII and Mother Teresa".
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Patent US6706479 - Bio-chip, photoluminescent methods for identifying biological material, and ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsA method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for...http://www.google.com/patents/US6706479?utm_source=gb-gplus-sharePatent US6706479 - Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chipsAdvanced Patent SearchPublication numberUS6706479 B2Publication typeGrantApplication numberUS 09/870,986Publication dateMar 16, 2004Filing dateJun 1, 2001Priority dateOct 5, 2000Fee statusLapsedAlso published asCA2424722A1, CN1484710A, EP1322787A1, EP1322787A4, US20020042070, WO2002029102A1Publication number09870986, 870986, US 6706479 B2, US 6706479B2, US-B2-6706479, US6706479 B2, US6706479B2InventorsRavi F. Saraf, Sanjun NiuOriginal AssigneeVirginia Tech Intellectual Properties, Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (22), Non-Patent Citations (15), Referenced by (4), Classifications (47), Legal Events (4) External Links: USPTO, USPTO Assignment, EspacenetBio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chipsUS 6706479 B2Abstract A method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for its single stranded nucleic acid sequence to bind to a material of interest, photoluminescence from the sensor can be measured. An apparatus for tagging-free detection of binding of molecules also is provided. Methods of making tagging-free sensors are provided. Also, tagging-free methods to detect binding of antigens and related devices are disclosed.
We claim: 1. A tagging-free method to detect the binding of untagged single stranded nucleic acid to an untagged material of interest, comprising the steps of:
(A) providing a sensor comprised of a first layer and a second layer wherein said first layer comprises an untagged single stranded nucleic acid and wherein said second layer comprises a photoluminescent material, and wherein said first layer and said second layer are separate layers, and wherein said second layer comprises material selected from the group consisting of aromatic polymers, doped or undoped metal oxides, sulfides, arsenides, tellurides, and nitride and phosphide nanocomposites; (B) exposing said sensor to a biological sample for sufficient time for said untagged single stranded nucleic acid to bind to an untagged material of interest in said biological sample; (C) applying light to said sensor; and (D) measuring photoluminescence from said sensor, wherein photoluminescence measured in said step of exposing is indicative of binding of said untagged single stranded nucleic acid to said untagged material of interest. 2. The tagging-free method of claim 1, wherein the single stranded nucleic acid is selected from the group consisting of DNA, RNA and PNA.
FIELD OF THE INVENTION The invention generally relates to nucleic acid, and more particularly, to binding of single stranded nucleic acid with biological material of interest in a sample to identify the material.
BACKGROUND OF THE INVENTION Nucleic acids such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and protein nucleic acid (PNA) are fundamental components of matter of living organisms. Nucleic acids generally speaking consist of certain constituent parts, or base pairs. The permutations in which these base pairs may be arranged is vast. Nucleic acid sequence analysis, i.e., determining the identity and sequence of the base pairs of a nucleic acid sample, is an important technology. Deciphering nucleic acid sequences is important for disease diagnosis, drug design and understanding of various biological mechanisms. Before 1996, traditional methods laboriously �read� the gene sequencing one base pair at a time.
Around 1996, Affymetrix developed a massively parallel sequencing approach, using a DNA chip with which several base pairs can be read simultaneously. A monolayer of specific single stranded DNA (ssDNA) fragments is assembled on an array of pixels (�1-100 μm2). The type of ssDNA may change from pixel to pixel. These ssDNA fragments act as �chemical tweezers� to pick the specific complementary tagged ssDNA from the sample to form double stranded DNA (dsDNA), i.e., a hybridization process occurs. The hybridized region is observed by a fluorescent label applied to the DNA in the sample before exposure to the ssDNA probe fragments. The process is speedier than single-base-pair methods, is specific, can analyze multiple nucleotide sequences, simultaneously can process hundreds of gene sequences and their alterations, and uses advantageously small chips. Practical applications of the parallel sequencing technology include: Affymetrix' study of p53 gene malfunction (i.e., mutation) responsible for cancer (especially breast cancer); Merck's study of changes in DNA sequencing as the cell beings to rapidly proliferate (to understand tumor formation); Incyte Pharmaceutical's disease-specific chips for drug design. Also, massively parallel, quick, sensitive and accurate bio-chip methods may boost the Human Genome Project.
However, Affymetrix' approach tags unknown to-be-sequenced DNA with a fluorescent dye�altering the sample so that it generally cannot be reused for other tests. Also, because the Affymetrix chip at each pixel has about at least a 5% error margin, the chip includes many repeated pixels, to manage the error margin. The fabrication process for Affymetrix' chip is expensive, requiring lithographic technique.
SUMMARY OF THE INVENTION It therefore is an object of this invention to provide methods and products for detecting the hybridization state of a nucleic acid molecule, without needing to tag the sample. The invention can be used to perform sequence analysis of unknown nucleic acids, such as DNA sequences. Several genes can be probed simultaneously. Sequencing according to the invention is relatively simple and quick, while providing precise and accurate sequencing information. The invention provides a bio-chip and other products for simultaneously analyzing one or more specific nucleic acid fragments (such as genes) in a solution.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS OF THE INVENTION In a first preferred embodiment, of which FIG. 1 is exemplary, the invention accomplishes nucleic acid (such as DNA, RNA or PNA) or protein sequence analysis without tagging (such as fluorescent dye tagging), and provides products and apparatuses useable for such tagging-free sequencing. Binding of molecules to a single stranded nucleic acid sequence can be detected without using conventional tagging. The invention uses a material that is photoluminescent, which broadly includes a material that glows at any wavelength or that luminesces because of photons. The invention uses application of light, and the light input may be ultraviolet, visible, infrared or other light, without limitation.
In a preferred embodiment, the single stranded nucleic acid of the present invention is attached to a photoluminescent material. A preferred example of the attachment is by grafting. �Grafting� may include covalently bonding a portion of the single stranded nucleic acid to the photoluminescent material (e.g., by an amine linkage from lysine or histidine), or it may include physical adsorption on the photoluminescent material, or other joining mechanism that associates the single stranded nucleic acid and the photoluminescent material in a manner which leaves a portion of the single stranded nucleic acid free to bond to other biological materials of interest. Examples of the photoluminescent material include, without limitation, an optically active thin film, aromatic polymers, matrix materials that include metal oxides or sulfides such as ZnS doped with Mn, etc.
It will be appreciated that sequencing according to the invention may be accomplished without any tagging of the DNA to be tested. This no-tagging feature will allow probing �before� and �after� structure of DNA when subjected to a stimulus. For example, mutation of a gene due to UV radiation can be analyzed by the disclosed DNA chip. A probe according to the invention can probe kinetics of the mutation processes, location of the mutation and, importantly (in the presence of a drug) evaluate the role of the drug-action on inhibition or acceleration of certain mutation processes.
The invention also in a further embodiment may be used to probe DNA �before� and �after� physical or chemical treatment, which may be particularly useful for drug design applications.
The self-alignment property is important in lowering the cost of device fabrication. For example, if 100�100 μm features at 1 mm period are made on a 25�25 mm substrate, over 600 combinatorial can be simultaneously performed. Such deposition geometry can be performed by existing dispensing systems common in microelectronics manufacturing or by micro-pipetting. The former is especially preferred because the process can be expedited to the order of 600 depositions in less than 100 seconds, with reliability. FIG. 4(d) shows the resulting surface with portions of two different types of ssDNA labeled ssDNA-1 and ssDNA-2.
Preferably the functional groups are highly polar to achieve selectivity. By functionalizing discrete regions of the polymer surface, the ssDNA can be grafted �locally� confined to these regions. Preferably these regions are about 100 by 100 microns so that it will be convenient to micro-pipette different ssDNA sequences in different surface modified regions. Thus an array of known ssDNA of various sequences can be deposited on the substrate 1. Because the functionalized surface is highly polar and the polymer (such as polystyrene) is non-polar the DNA solution will self align on the functionalized regions due to surface tension.
In an alternative embodiment of the invention, the optically active layer 2 of the device of FIG. 1 instead of being a nanocomposite is a photoluminescent polymer. Such a device may comprise a two-layer structure of a monolayer of ssDNA grafted onto a polymer thin film. The polymer thin film may be one that is optically active such that it has fluorescence when excited by radiation in the UV region, preferably in the vicinity of 265 nm wavelength. For example, the film may comprise polystyrene. The film may be deposited on an appropriate substrate depending on the intended end-use of the probe application. When the device is exposed to UV light (�265 nm wavelength), the photo-active polymer film will emit light and the device may be used as set forth above for a device in which the optically active layer 2 is a nanocomposite.
Next, the block copolymer is subjected to a surface treatment that etches the surface to expose the discrete phase and in the same process activates the surface of the discrete phase. However, the etched �matrix� polymer is not activated. Subsequently, the structure is exposed to precursor solution of inorganic and/or organo-metallic salts in a solvent system that does not attack the block copolymer. The salt(s) react with the activated surface to form a selective deposition on the exposed surface of the discrete phase.
FIG. 14 shows a schematic of an exemplary process flow to make a chip according to the invention. Polymeric �blobs� 6 ranging in size from about 10-1000 nm are produced on a substrate 1. The blobs may be on a random or periodic array (see FIG. 14(a)). Subsequently a single stranded nucleic acid molecule 3 a is attached (such as by grafting) to each blob 6. Depending on the size of the blob and the number of bases in the nucleic acid sequence 3 a, the number of nucleic acid sequences 3 a per blob 6 may range from about 1 to 10. For blob diameters below 50 nm and nucleic acid molecules having more than 80 bases, the number of nucleic acid molecules is likely to be one per blob.
In FIG. 15(b), the block copolymer thin film 8 is spin cast and annealed to form a monolayer of the microphase separated spheres. Then the matrix is etched by ozone or plasma to form isolated A-spheres 8 a. Subsequently the sphere 8 a is annealed above the glass transition temperature of the polymer to form polymer �blob� 88 (see FIG. 15(c)). In the next step, the surface 1 a of the substrate 1 is modified by anionic groups (see FIG. 15(d)) to provide modified blobs 88 a. The group used for such modification may be a strong anion that forms a salt when exposed to an aqueous solution of Zn, Cd, Pb or Hg salts. Upon annealing (see FIG. 15(e)) to form annealed blobs 88 b and exposure to H2S, II-VI calcogenid nanoparticles 9 will be formed (see FIG. 15(f)) in the blobs 88 c. To provide the highest photoluminescence, a typical nanoparticle may be doped, such as, ZnS:Mn or ZnS:Ag. The former has highest photoluminescence intensity around 265 nm which is close to the maximum absorption of nucleic acids in the UV-region.
A �digital� approach may be used in fabricating a bio-chip according to the invention, in which the probe sites are arranged on a periodic array with 10-50 nm scale periodicity. A discrete, periodic arrangement will allow for absolute nucleic acid count with high sensitivity and accuracy, and low noise/error that otherwise arises due to overlap of signals from adjacent fragments in an �analog� method. Compared to conventional bio-chip technologies, the inventive methods and products are useable with sample sizes 103-104 smaller. Also, the array fabrication may be based on a relatively inexpensive and highly parallel self-assembly approach instead of expensive lithographic techniques.
EXAMPLE 1 A block copolymer film of polystyrene (PS)/polybutadiene (PB) was deposited on a silicon (Si) surface to produce a highly ordered discrete phase of PS spheres. See FIG. 8. The structure was then exposed to plasma containing water vapor to etch the butadiene and modify polystyrene. FIG. 9 shows a typical etch curve (for polybutadiene) indicating the amounts of etch as a function of exposure time. To confirm that the surface modification is indeed selective, pure polymer PS and PB were exposed to similar treatment. The wetting angle for water, in FIG. 10, as a function of plasma exposure clearly indicates that PS is modified and becomes highly polar (i.e., wetting) however, PB is not modified in any significant manner. Particularly, FIG. 10 shows that the contact angle of water decreases on PS but remains high for PB as the treatment time increases. The activated surface is immersed in Zn-salt solution and exposed to H2S to form ZnS. FIG. 11 shows a typical field-emission scanning electron micrograph (SEM) where the PS spheres are bright compared to the PB matrix. The brightness of PB is attributed to presence of high atomic number Zn compared to low atomic weight materials (i.e., C and H) in the matrix. In FIG. 11, ZnS nanoparticles on a block copolymer are shown, and notably the PS discrete islands are spherical and bright compared to the matrix indicating that the ZnS is confined only to PS. The random arrangement of PS spheres is due to over-etching of PB. Photoluminescence (PL) spectra of the resultant sample shown in FIG. 12, which shows photoluminescence from ZnS particles, indicate that the typical emission peak at �420 nm is strongly blue shifted by over 100 nm indicating the presence of nanoparticles of ZnS. The strong blue shift is an indication of quantum confinement effects indicating a nominal size of <3 nm ZnS particles.
EXAMPLE 2 Using self-assembled block copolymer monolayers and high anisotropic etching, a structure is made by grafting ssDNA fragments (x) on a nanoparticle coated polymer blob. The blobs (made of polystyrene (PS)) are arranged periodically on a (silicon) substrate. The periodicity and size of the blobs is in the 10-20 nm range (see FIG. 14(a)). The direct band gap semiconductor nanoparticles are embedded in-situ in the polymer to ensure nominally monodispersed size distribution and to simplify the process. The luminescence excitation will be tailored to the maximum at �260 nm (close to the absorption maxima of DNA). As the ssDNA is grafted, the luminescent light in the visible range will be attenuated from I0 to Ii. Thus by measuring the difference ΔI=If−Ii as the probe sites with x grafts are exposed to a ssDNA mixture the amount of y fragments can be quantitatively calculated. The calibration constant relating ΔI and number of y attached to the substrate is determined exactly since the number of x sites are known. To achieve measurable contrast, i.e., ΔI�10% change, the minimum ssDNA size is a 50 base pair sequence. For 50 base sequence ssDNA on 20 nm pitch array, only one chain per blob is possible due to lateral size of the gaussian chain (see FIG. 14(a)). A total array size of 104 μm2 is sufficient to measure I0, Ii and If for nanoparticle monolayers with a conservative quantum efficiency of 25% and detector acceptance angle of 30�. This way, a sensitivity to measure 2.5�107 ssDNA (or �10 femotgram) per array is possible. By making an ensemble with several arrays of different ssDNA grafts and connecting each array to a 100 μm diameter optical fiber, a parallel analysis of several fragments can be achieved.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS4820649Sep 22, 1987Apr 11, 1989Teijin LimitedMethod and kit having layered device for detecting biological component by interference colorUS5194393Nov 8, 1990Mar 16, 1993Bayar AktiengesellschaftOptical biosensor and method of useUS5552272Jun 10, 1993Sep 3, 1996Biostar, Inc.Medical diagnosisUS5593867Sep 23, 1994Jan 14, 1997Becton, Dickinson And CompanyFluorerscence polarization detection of nucleic acid amplicationUS5629214May 31, 1995May 13, 1997Biostar, Inc.Methods for forming an optical device for detecting the presence or amount of an analyteUS5707799Sep 30, 1994Jan 13, 1998Abbott LaboratoriesDevices and methods utilizing arrays of structures for analyte captureUS5711915Oct 24, 1995Jan 27, 1998Bayer AktiengesellschaftOptical solid-phase biosensor based on polyionic layers labelled with fluorescent dyesUS5786139Dec 9, 1994Jul 28, 1998Panvera CorporationMethod and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tagUS5831259 *Jun 27, 1995Nov 3, 1998Commissariat A L'energie AtomiqueElectrooptical transducer utilizing photoluminescent conjugate oligomersUS5837196Jan 26, 1996Nov 17, 1998The Regents Of The University Of CaliforniaHigh density array fabrication and readout method for a fiber optic biosensorUS5849547Jul 26, 1994Dec 15, 1998Bio MerieuxMethod for nucleic acid amplification by transcription using displacement, and reagents and kit thereforUS5858653Sep 30, 1997Jan 12, 1999Surmodics, Inc.Reagent and method for attaching target molecules to a surfaceUS5955377Apr 17, 1995Sep 21, 1999Biostar, Inc.Screening for compounds in a sample using transmitted or reflective light which measures mass change on surface of deviceUS5962218 *Nov 24, 1993Oct 5, 1999Igen International Inc.Incubating sample, electrochemiluminescent label, means for binding, and particles which bind analyte or product to form complex; collecting on surface; inducing label to luminesce by surface selective excitation; detecting or quantitatingUS5981185May 28, 1997Nov 9, 1999Beckman Coulter, Inc.Determining size of duplicated nucleotide repeats in sample; bringing sample into contact with an array of oligonucleotides attached to a support, hybridization, eluting hybrids from support, sizing nucleotide repeatsUS6045996May 16, 1996Apr 4, 2000Affymetrix, Inc.Hybridization assays on oligonucleotide arraysUS6060237Jan 17, 1995May 9, 2000Biostar, Inc.Devices and methods for optical detection of nucleic acid hybridizationUS6117529 *Dec 18, 1996Sep 12, 2000Gunther LeisingOrganic electroluminescence devices and displaysUS6241819 *Apr 20, 1993Jun 5, 2001North American Philips Corp.Method of manufacturing quantum sized doped semiconductor particlesUS6284503 *Jan 15, 1997Sep 4, 2001University Of Utah Research FoundationComposition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfacesUS6297018Jan 28, 2000Oct 2, 2001Ljl Biosystems, Inc.Locating sample at examination site, illuminating with polarized light, detecting light emitted only from volume located away from sample boundary interfaces, then analyzing dataWO2002029102A1Oct 1, 2001Apr 11, 2002Virginia Tech Intell PropBio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips* Cited by examinerNon-Patent CitationsReference1A Steptavidin Surface on Planar Glass Substrates for the Detection of Biomolecular Interaction Analytical Biochemistry, vol. 282, No. 2, (Jul. 1, 2002).2Absorption of Avidin on Microfabricated Surfaces for Protein Biochip Applications, Biotechnology and Bioengineering, vol. 73, No. 4 (May 20, 2001).3Chemiluminescent Immunoenzyme Biosensor with a thin-layer Flow-through Cell: Application for Study of a Real-Time Bimolecular Antigen-Antibody Interaction, Biosensors & Bioelectronics, vol. 11, No. 9 (1996).4Considerations for the Quantitative Transductioni of Hybridization of Immobilized DNA, Analytica Chimica Acta, vol. 400 No. 1-3, (Nov. 22, 1999).5Detection of Conformation Changes in a Immobilized Protein Using Surface Plasmon Resonance, Anaylitcal Chemistry, vol. 70, No. 10 (May 15, 2998).6Direct Bioelectrochemical Monitoring of Choline Oxidase Kinetic Behavior in Langmuir-Blodgett Nanostructure, Bioelectrochemistry and Bioenergetics, vol. 46, No. 1 (Aurg. 1998).7Fixed Polarizer ellipsometry for simple and sensitive detection of thin films generated by specific molecular interactions: applications in immunmoassays and DNA sequence detection, Clinical Chemistry 2031-2035 (1998) R. M. Ostroff, D. Maul, G. R. Bogart, S. Yang, J. Christian, D. Hopkins, D. Clark, B. Ttotter, and G. Moddel.8Genetic Engineering of a Single-Chain Antibody Fragment for Surface Immobilization in an Optical Biosensor, Biosensors & Bioelectronics, vol. 13, No. 3-4 (Mar. 1, 1998).9High-Throughput Assays on the chip based on metal Nano-Cluster Resonance, Journal of Pharmaceutical and Biomedical Analysis, vol. 24 No. 5-6 (Mar. 2001).10Immobilization of Nucleic Acids at Solid Surfaces: Effect of Oligonucleotide Length on Layer Assembly, Biophysical Journal, vol. 79, No. 2 (Aug. 2000).11Immobilization of Ultra-Thin Layer of Monoclonal Antibody on Glass Surface, Journal of Chromatography, vol. 566, No. 2 (May 31, 1991).12Immunosensors Technology and Opportunities in Laboratory Medicine, Clinical Chemistry, vol. 42, No. 2 (1996).13Montioring of the Refolding Process for Immobilized Firefly Luciferase with a Biosensor Based on Surface Plasmon Resonance, Journal of Biochemistry vol. 129, No. 1 (Jan. 2001).14Optical Biosensors for Real-Time Measurement of Analytesin Blood Plasma, Biosensors and Bioelectronics, vol. 17 No. 8 (Aug. 2002).15Processing of cDNA and Genomic Kilobase-Size Clones for Massive Screening, Mapping and Sequencing by Hybridizatiom, Research Report, Snezana Drmanac and Radoje Drmanac; Argomme National Laboratory, Argonne, Il, BioTechniques vol. 17 No. 2 (1994).Referenced byCiting PatentFiling datePublication dateApplicantTitleUS7075428Aug 18, 2005Jul 11, 2006Biowarn LlcMethodology and apparatus for the detection of biological substancesUS7200254 *Sep 5, 2002Apr 3, 2007Ngk Insulators, Ltd.Probe reactive chip, sample analysis apparatus, and method thereofUS7989229Sep 10, 2007Aug 2, 2011International Business Machines CorporationTactile surface inspection during device fabrication or assemblyUS8263502 *Aug 11, 2009Sep 11, 2012Synos Technology, Inc.Forming substrate structure by filling recesses with deposition material* Cited by examinerClassifications U.S. Classification435/6.11, 117/68, 435/91.1, 250/214.1, 428/209, 435/6.12International ClassificationG01N33/566, C12Q1/68, C12M1/00, C12N15/09, C40B40/10, G01N33/543, G01N33/53, G01N21/78, G01N21/64, G01N37/00, G01N21/76, C40B40/06, C40B60/14Cooperative ClassificationB01J2219/00274, B01J2219/00529, B01J2219/00635, B01J2219/00725, B01J2219/00729, B01J2219/00497, B01J2219/00576, B01J2219/00637, B01J2219/00432, C12Q1/6825, B01J2219/00619, C40B40/06, B82Y30/00, B01J2219/00585, B01J2219/00722, B01J2219/00612, C40B40/10, C12Q1/6837, C40B60/14, B01J2219/00707, B01J2219/00677, B01J2219/00596, B01J2219/00626, B01J2219/00578, B01J2219/00659, B01J2219/00608European ClassificationB82Y30/00, C12Q1/68B2HLegal EventsDateCodeEventDescriptionMay 6, 2008FPExpired due to failure to pay maintenance feeEffective date: 20080316Mar 16, 2008LAPSLapse for failure to pay maintenance feesSep 24, 2007REMIMaintenance fee reminder mailedJun 18, 2002ASAssignmentOwner name: VIRGINIA POLYTECHNIC INSTITUTE & STATE UNIV., VIRGFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SARAF, RAVI F.;NIU, SANJUN;REEL/FRAME:013004/0953;SIGNING DATES FROM 20011005 TO 20011006Owner name: VIRGINIA TECH INTELLECTUAL PROPERTIES, INC., VIRGIFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:VIRGINIA POLYTECHNIC INSTITUTE & STATE UNIVERSITY;REEL/FRAME:013004/0965Effective date: 20011026Owner name: VIRGINIA POLYTECHNIC INSTITUTE & STATE UNIV. 146 HFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SARAF, RAVI F. /AR;REEL/FRAME:013004/0953;SIGNING DATES FROM 20011005 TO 20011006Owner name: VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. 1872 PFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:VIRGINIA POLYTECHNIC INSTITUTE & STATE UNIVERSITY /AR;REEL/FRAME:013004/0965RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services©2012 Google

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Simple Snap-to-Grid cursor to your graphics application - CodeProject 11,331,883 members (35,297 online)
my email:franklinwa@163.com Last Visit: 31-Dec-99 19:00 Last Update: 27-Mar-15 12:01Refresh1

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Cougs gear up with o-line push & sackmasters - Washington State - Scout
Cougs gear up with o-line push & sackmastersBRAULIO PEREZ09/02/2009PULLMAN -- The fire was there for the Cougars from the beginning with countless reminders of the season being only a few days away. Unfortunately, those reminders also included the injury bug. The offensive line had a good day in the run game, and Kevin Lopina and Marshall Lobbestael were on target. Meanwhile, Travis Long and Jason Stripling went and got themselves a little somethin'-somethin'.Sports Information's Joe Nickell said that Tyree Toomer is having surgery on his torn pectoral muscle and will be out for the season. The safety had played well and was expected to be a contributor behind starters Chima Nwachukwu and Xavier Hicks.
Toomer's absence likely means a larger reserve role for LeAndre Daniels, who has also come in for praise from the coaches in the latter half of fall camp.
And with Daniels back and a few players out with the flu, including Aire Justin, who is listed as an either/or with starter Daniel Simmons at cornerback, Nwachukwu took a number of snaps at the right corner spot opposite Brandon Jones.
THE DAY BEGAN with warm-ups and everyone was into it -- the coaches saying; "Martin Stadium!" to the players, "Game Week!" coming back from players, along with an occasional, "It's a great day to be a Coug!" from senior RB Dwight Tardy.
Kevin Lopina saw most of the action running with the No. 1 offense on Tuesday, while redshirt sophomore Marshall Lobbestael spent most of his day directing the second team. Paul Wulff has said he would alternate both of his quarterbacks throughout Saturday's matchup against Stanford but also said if someone gets a hot hand, he'll stay in.
Neither Lopina nor Lobbestael threw an interception in the practice.
WHILE THE OFFENSIVE line did a good job with the run game in the first practice of the game week, the pass protection towards the end of practice was not their best.
Freshman DE Travis Long "sacked" Lopina and during the same series, LB Jason Stripling got in through the middle untouched and forced Lopina to abruptly check down to RB Logwone Mitz, who couldn't haul the ball down in front of S Xavier Hicks. The offensive line did do better in the run game, however, and the Cougs had a solid day overall executing their assignments.
"We had an above average Tuesday practice," said co-defensive Coordinator Jody Sears. "I liked the..execution of it. As a coach you want to consistently drive them and coach them to keep that intensity -- which I think might be the one thing that we've most improved on."
WITH MIDDLE 'BACKER Alex Hoffman-Ellis, strong-side LB Andy Mattingly and weak-side LB Jason Stripling slated to start on Saturday, freshman MLB Darren Markle and sophomore weakside LB Louis Bland continued to get a lot of reps in with the 1's.
And Sears said you never know how the starting unit can change from Tuesday to Saturday because of injury and other factors.
"Oh absolutely, there's always a chance for the guys to work their way in," said Sears. "It's what's best for the team and I think the kids understand that, there doesn't seem to be much selfishness along those lines."
WR Kevin Norrell, CB Aire Justin, and CB Terrance Hayward were among those off the practice field with the flu. ON THE RETURN side, C Andrew Roxas was back with his teammates in a practice jersey. He looked much, much thinner than his usual 300-plus pound self. Roxas, who has been ill over the summer, was diagnosed with a form of Viral Hepatitis while back home in California, said head trainer Bill Drake. The virus appears to be gone and he's on the mend, but he's lost over 30 pounds and will likely redshirt this season.
ROXAS FELLOW O-LINEMEN showed continued progress with the run game, something that did not go overlooked by the running backs.
"Our offense is doing well, our o-line is getting stronger, getting smarter. Guys are finally getting technique down and we know what we're supposed to be doing out there," said Tardy.
The offense focused a majority of the plays towards the end of practice's 11-on-11 in the run game. Tardy, James Montgomery, and Logwone Mitz all saw time running with the 1's. Tardy was named the starter this week, but all could get plenty of turns.
"It's great for me, the offense is going to have fresh legs in at all times," said Tardy. "You have to have guys competing out there and when you have guys competing it brings the best out of you and we have three great backs, so I think it's great for the offense."
That doesn't mean Tardy's goals have changed.
"I have to get over 1,000 yards. I've been trying to get it every year but I've had some setbacks and shortcomings but I feel like it's a good year for me. And I'm going to go out there every day and try my hardest." said Tardy.
Share on Facebook Share on TwitterTrending on ScoutLeach on CFC TV: I’ll take John Wayne any dayWily vet Ivana Kmetovska keeping dream aliveA first at linebacker this spring at WSUIsaac Dotson: WSU's Swiss Army knifeCougar O a bit rusty on Day TwoShare & EmbedDaugherty emotional as Cougs win again Full StoryCougfan Top StoriesFeel the love, says June D. Cougs win again!THE ICE-WATER-in-their veins road warriors otherwise known as the Washington State women's basketball team did it again today, defeating Iowa's Hawkeyes in front of 5,000 Iowa City…by the staff of Cougfan.comCougfan2:27 PMIsaac Dotson: WSU's Swiss Army knifePULLMAN -- Isaac Dotson, the Cougs' senior WILL linebacker, has seen just about every corner of the defense under Washington State defensive coordinator Alex Grinch.by Zach AndersCougfan12:30 PMLeach on CFC TV: I’ll take John Wayne any dayPULLMAN – Mike Leach tends to be at his most loquacious when discussing subjects other than football. Take movies, for example. In a brief post-practice press confab, Leach talked…by Zach AndersCougfanYesterday at 6:02 PMCougar O a bit rusty on Day TwoPULLMAN -- After an unseasonably balmy first session, the Cougars’ second spring practice was met with rain and even a little hail. And it seemed that poor weather threw off the…by James LindsayCougfanYesterday at 3:59 PMWily vet Ivana Kmetovska keeping dream aliveJUNE DAUGHERTY'S scrappy guards have earned a ton of attention in the Cougars' deep post-season run but come Sunday in a noon Pacific time tip off at Iowa in the WNIT Elite Eight,…by the staff of Cougfan.comCougfanYesterday at 11:06 AMA first at linebacker this spring at WSUCOUGAR LINEBACKERS COACH Ken Wilson has something for the first time in his five years at Washington State. And he’s smiling ear to ear about it this spring.by Barry BoltonCougfanFriday at 2:55 PMWhy fans should chill if a kid decommitsA CASUAL GLANCE at community message boards, both here in Cougarville and around the college football world, leads one to an inescapable conclusion: Fans take it hard when a…by Barry BoltonCougfanFriday at 12:16 PMCFC TV: Cody O'Connell's new rolePULLMAN – A year ago Cody O'Connell was a guy who had never started a game. So what was it like for the fifth-year senior All-American to take on a leadership role and work with…by Zach AndersCougfanFriday at 9:30 AMDaugherty emotional as Cougs win againPULLMAN -- When the game was done, and Washington State's women's basketball team had earned itself a spot in the WNIT Elite Eight, the Beasley Coliseum crowd of more than 1,400…by Zach AndersCougfanThursday at 10:59 PMWSU Spring Ball: Day OnePULLMAN -- In WSU’s first practice since December’s Holiday Bowl, the young Cougar receivers tried to show that the impact losing of veterans River Cracraft and Gabe Marks will be…by James LindsayCougfanThursday at 9:12 PMCFC TV: Leach says Cougs already aheadPULLMAN – Spring ball on the Palouse is only one day old but already, Mike Leach said it feels like the Cougs are ahead of the pace from last year. One big reason?by Zach AndersCougfanThursday at 8:50 PMWSU looking in-state for Pelluer 2.0BACK WHEN Peyton Pelluer signed with WSU in 2013, few knew he would lead the Cougs in tackling two of the last three years. Now, though, the Cougar coaches are eyeing his potential…by Braulio PerezCougfanThursday at 3:59 PMLoad More

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Terry: Things can only get better | Daily Mail Online
Last updated at 13:11 18 June 2006
John Terry insisted England were in a good position after winning their opening two fixtures and was buoyed by the fact they were top of the group despite not having performed anywhere near their potential.
The 25-year-old has been one of England's most impressive performers at the centre of defence alongside Rio Ferdinand with the team coming in for criticism following their less than convincing performances.
Terry rebuffed the censure, telling Sky Sports News: "Two out of two and six points - it can't be much better and let's be encouraged that things can only get better from a performance point of view."
The Chelsea defender also sang the praises of strikers Wayne Rooney and Michael Owen, the latter having been the subject of some criticism following his below-par performances which have seen him substituted in both games.
"Marking him (Rooney) is a nightmare and he is looking sharp and ready to go.
"Owen would tell you he had 360 minutes in him as long as he playing, his touch is good and he looks sharp."
Eriksson is expected to change his midfield with both Steven Gerrard and Frank Lampard on a yellow card but Terry insists they will be going all out to win Group B.
"When you got Lamps and Stevie G - two of the best midfielders in the world - in front of you it doesn't get much better than that.
"We want to top the group. It is important to go in top and win the game.
"There is a record to be set straight as we've not beaten them for a while.
"We are in a very good position so it's up to manager to go into it and rest players. But we want to keep the winning mentality."

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See Map Get an overview of this hotelBrooklyn hotel in Red Hook with free breakfast and fitness center Location
High-speed wired and wireless Internet access is complimentary. Business-related amenities consist of a 24-hour business center and limo/town car service. A complimentary breakfast is offered. This Brooklyn hotel also offers a fitness center, multilingual staff, and coffee/tea in a common area. Limited onsite parking is available on a first-come, first-served basis (surcharge). Comfort Inn Red Hook is a smoke-free property. Free wired high-speed Internet 24-hour front desk Number of floors - 4 Coffee/tea in common areas Free newspapers in lobby Free breakfast Elevator/lift Fitness facilities Free WiFi Total number of rooms - 81 Limo or Town Car service available Multilingual staff Parking (limited spaces) Luggage storage 24-hour business center Self parking (surcharge) Smoke-free property Television in common areas Show all hotel amenities

value: This website is an online travel agency owned by Expedia Group, an American online travel shopping company based in Seattle.


text:
Down Under Street Team on Etsy
› Down Under Street Team's profile
Down Under Street Team's Profile
If you're interested in joining our team, please visit our team page - http://www.etsy.com/teams/5156/dust - read up, we do like our members to participate, and chat in the team threads. We used to charge $2 per year membership, to maintain our own website. At the moment we're not, but we may again in the future.
team :: http://www.etsy.com/teams/5156/dust
website:: http://www.dustteam.com/
blog :: http://www.dustteam.com/blog/
Follow us on Twitter:: https://twitter.com/AusEtsyTeamDUST
If you use Firefox you can get an addon -…
If you use Firefox you can get an addon - https://addons.mozilla.org/en-US/firefox/addon/190 that turns text links into real links!

value: This website is an American e-commerce company focused on handmade or vintage items and craft supplies.


text:
Code Project Rating and Reputation FAQ - CodeProject 11,790,021 members (38,713 online)
Last Visit: 31-Dec-99 18:00 Last Update: 3-Oct-15 23:32Refresh1


text:
Abahana Villa Ciervo, Fanadix, Spain - Booking.com
http://www.booking.com/hotel/es/ciervo.html
Calle Cabo Finisterre, 03724 Fanadix, Spain
Featuring a private swimming pool, Ciervo is located in the residential area of Cap Blanc, between Calpe and Moraira. The villa offers free Wi-Fi and is 650 feet from the sea.
Next to the pool, guests can enjoy a terrace with sun loungers a covered dining table and BBQ facilities. There is also a garden and private parking for several vehicles.
A small shopping centre, including a supermarket and some restaurants, can be found within a 5-minute walk. L’Ampolla Beach is 1.2 miles from the property. Alicante Airport is 50 minutes’ drive away. We speak your language!
Abahana Villa Ciervo has been welcoming Booking.com guests since Dec 13, 2013
When would you like to stay at Abahana Villa Ciervo?
5 Reasons to Choose Abahana Villa Ciervo
Facilities of Abahana Villa Ciervo
Please note that check-in and key collection take place at: Centro Comercial Moravit, Carretera Calpe-Moraira, 306 , Moraira 03724.
Please inform Abahana Villa Ciervo of your expected arrival time in advance. You can use the Special Requests box when booking, or contact the property directly using the contact details provided in your confirmation.
A damage deposit of EUR 700 is required upon arrival – that's about 743.82USD. This deposit is fully refundable at check-out as long as there's been no damage to the property.
License number: VT434970A
We need at least 5 reviews before we can calculate a review score. If you book and review your stay, you can help Abahana Villa Ciervo meet this goal.
213287,212666|1,204587,211631,210858,209825,200322,209175,212545,210963,209640,213287|4,212500,210858|4,209865,212911,194153,207483,207205,210817,209477,193924,210898,208943,207473,203218,211007,203220,208334,212913,213116,211461,204809,212371,206882,210718,210941,201691,211506,212498,212765,213287|1,210648,203056,209853,213398,205677,212841,202145,212912,203454,204742,201380|3,208823,204534,208414,209618,211158,210424,203206,206930,209104,208689,210785,205512,202398,204377,210113,197382,210890,207723,203225,208782,212976,211643,201380,210890|1,212683,213052,207184,209049,210994,211095,213406,212019,201964,208044,212765|1,213203,207092,208493,203396,212666,202717,209923,204807,200404,211090,213285,212877,203454|1,208579,202643,210784,207884,206793,204321,213104,211589,205164

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text:
Kiddies on Pinterest | Kid Crafts, Turkey Hat and Thanksgiving Crafts
Jessie Bailey Bell
for da lil ones
Inspiration Bloggers, Crafts Ideas, Creative Ideas, Easy Crafts, Kids Ideas, 5Th Birthday, Craft Ideas, Kids Fun, Bloggers Network
DIY Christmas Gifts for Kids | Best Kids Crafts and DIY Kid Craft Ideas diyready.com/...
Crafts For Kids, Kids Diy, Kids Stuff, Kids Activities, Kids Crafts, Crafts Kids, Creative Kids, Things Kids, Kids Kraft
Best Kids Craft Ideas http://diyready.com/crafts-for-kids-toilet-paper-roll-craft-projects/
Crafts for Kids Using Toilet Paper Rolls http://diyready.com/crafts-for-kids-toilet-paper-roll-craft-projects/
FREEBIE - BME Turkey Hats
The Razzle Dazzle Classroom: Thanksgiving FUN!
Kids Thanksgiving, Diy Crafts, Handprint Turkey, Easy Thanksgiving Crafts, Preschool Thanksgivingcrafts, Soooooo Coool, Fall Crafts For Little Kids, Toddlers Crafts, 30 Easy
Footprint Turkey, Toilet Paper Roll Turkey, Handprint Turkey, Fall Leaves Turkey
Over 30 Easy Thanksgiving Crafts for Toddles and Preschoolers #thanksgivingcrafts
Easy toddler crafts for the baby!
More than 30 easy Thanksgiving crafts that are perfect for toddlers, preschoolers and little kids! #thanksgiving #crafts
from etsy.com Iron on Thanksgiving Shirt PDF - Gobble Iron on Transfer / Kids Boys Turkey…
Sewing, Baby Ideas, Fall Thanksgiving, Boys Turkey, Baby Girls, Kids Thanksgiving Shirts, Crafty Ideas, Baby Stuff, Wall Ideas
Girlie Ideas, Christmas Time, Elf On Shelf For Girls, Countdown To Christmas Ideas, Elf On The Shelf Girl Ideas, Girl Elf On The Shelf, Elf On The Shelf For Girls, 25 Elf On The Shelf Ideas, Army Men
Elf on The Shelf for Girls.
Elf on The Shelf is a fun way to countdown to Christmas. I have a daughter who doesnt have army men or legos, so I needed Elf on The Shelf Ideas for Girls.
25 Elf On The Shelf Ideas For Girls {totallythebomb.com}
Christmas Ideas Kids Diy, Fun Holiday Crafts For Kids, Christmas Kids Craft, Christmas Fun Kids, Christmas Ideas With Kids, Christmas Ideas For Preschool, Christmas Crafts Kids To Make, Christmas Activities For Kids, Christmas Fun Ideas
Winter Snowflake Homemade Slime - Little Bins for Little Hands
A-mazing! Cheerios decorated to be left out for Santa's elves! he he
Gingerbread Sc Playdough, Dough Recipe
Homemade Gingerbread Play Dough Recipe - very close to Rachelle's trusted recipe, just adding gingerbread spices
Hands Prints, Daycares Crafts, Crafts Ideas, Christmas Crafts, Kids Crafts, Hands Crafts, Holiday Crafts, Art Activities, Reindeer Crafts
Reindeer art activity.
A Cupcake for the Teacher: reindeer

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Prescott Line#42 RBSophomoreSMU MustangsHometownOxford, MIHeight6-0Weight228 lbs.2014 SeasonATTYDSTD782564Career1685887Go toPrescott Line
Player ProfileStatsSplitsGame LogPhotosNEXT GAMEESPNGerald J. Ford StadiumFri, Sept 47:00 PM ETBaylor(0-0)@SMU(0-0)Conversation »Tickets » RUSHINGRECEIVING2014 SPLITSATTYDSAVGLNGTDRECYDSAVGLNGTDSeason782563.317410414.1100Home411092.71217304.3100September12242.040122.020Complete Splits »
CAREER STATSRUSHINGRECEIVINGSEASONATTYDSAVGLNGTDRECYDSAVGLNGTD2014782563.317410414.11002013903323.721311565.11302014 Game LogRushingReceiving
Traylon Shead arrived at Texas back in 2010 with great expectations already attached to him as one of the most highly touted recruits in the country.But Shead never really got his career going with the Longhorns.
about 795 days ago | Andrea Adelson | ESPN.com

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Papaya Women's Cotton Jumpers without Fastening | eBay
Papaya Women's Cotton Jumpers without Fastening
Ending Sunday at 8:30PM BST4d 11hBrand: Papaya
New listingBNWT Ladies 16-18 Brown Multi Longer Length Jumper
New listingLADIES JUMPER SWEATSHIRT Size M 46” PAPAYA LEISURE Maroon + FREE GIFT
Ending 30 Apr at 7:55AM BST4d 23hBrand: Papaya
New listingPapaya Weekend Ladies Size 18 Beige Boat Neck Jumper Spring Wear Curve Fashion +
Ending 4 May at 9:10PM BST9d 12hBrand: Papaya
*PAPAYA* MATALAN-LONG SLEEVE HOODED JUMPER-16-PINK/IVORY STRIPE-NEW
NEW COTTON PAPAYA GREY MARL LACE FRONT SWEATSHIRT JUMPER SIZE 16
LACE DOWN THE FRONT. SIZE 16.
Papaya Jumper Grey/White/Black Marl Netted/Crochet/Sheer 3/4 Sleeves UK Size 14
Lovely Netted Jumper with Stretch & 3/4 Sleeves.
Ribbed, knitted jumper. Crew neck. Auctiva's FREE Counter. 100% Cotton.
Ribbed, knitted jumper. Crew neck. Ideal for work and casual. Size 12/14. Used - in very good condition.
Papaya blue 3/4 ribbed stretchy sleeve soft crew neck sweater jumper OnSize (L)
Underarm to cuff: 12.5“ / Underarm to underarm across bust: 27“ / Back of neck to hem: 29.5“. 86% cotton / 14% Nylon.
PAPAYA Womens Jumper Size 10 UK V Neck Pullover Long Sleeve Cardigan Beige Ombre
Style: Ombre. Long Sleeves, V Neck Ladies Jumper. Size: 10 UK. Material: 60% Acrylic & 40% Cotton.
Papaya Rib Jumper In Airforce Blue Size 16 Chest 38” - 40” Stretchy
Crew Neck Nice Jumper. Chest 38” - 40”. Back Neck to length 23.5”. Good clean condition had been used ideal for dog walking jobs about the house Not for best. Shoulder to length 23.5”. Underarm to side seam length 15”.
Papaya Womens Black 100% Cotton Jumper Size 10 (69)
papaya size 14 grey medium knit ribbed jumper vgc
G9. Very good condition ribbed with stretch 18/19 inc from arm pit to arm pit and 36 inc waist and 23 inc long
PAPAYA WEEKEND MATALAN 12 14 MEDIUM M BLUSH PINK 100% COTTON JUMPER 3/4 SLEEVES
BLUSH PINK. REALLY BEAUTIFUL JUMPER.
AQUA STRIPED JUMPER. NICE AQUA GREENS AND WHITE STRIPES ON JUMPER. DEEP V AT THE NECKLINE. TURN UP CUFFS. LONG SLEEVES. BY PAPAYA. SIZE 10. 100 % COTTON. OFFERS WELCOME. MORE ITEMS FOR SALE. PLEASE TAKE A LOOK. I ALWAYS OBTAIN PROOF OF POSTAGE. PLEASE NOTE P&P COVERS PACKAGING AND OTHER COSTS RELATING TO THIS SALE. THANK-YOU FOR LOOKING. HAPPY BIDDING. GOOD LUCK.
PAPAYA - LADIES JUMPER - SIZE 8 - NEW WITH TAG
LADIES JUMPER. COLOUR - SEA GREEN, SEE PICTURE. SIZE 8. NEW CONDITION. LONG SLEEVED.
Papaya Women's Beige/Neutral/Stone Lightweight Jumper. Size 18
Ending Friday at 8:47PM BST2d 11hBrand: Papaya
pappaya size 14 black/ white 100% cotton thin knit long jumper vgc
K5 18inc from arm pit to arm pit 36inc wide 33inc long floral front with sequins.
charcoal grey ribbed 3/4 sleeve waist length jumper size 18 with 3 button detail
Charcoal grey ribbed cotton jumper with 3/4 sleeve and 3 button detail by Papaya. Rarely worn
Papaya Black Vest Jumper - Size 8
Papaya Vest Jumper Black Size 8 V neck Used item but in excellent condition Comes from a smoke free and pet free home. Please check out my other items as happy to combine postage
Ending Sunday at 5:05PM BST4d 8hBrand: Papaya
Classic cream & blue nautical stripe look. Shoulder to hem 25". Long sleeve. Shoulder to cuff 26.5". Waist 42". Pure cotton.
Papaya Womens Grey 100% Cotton Jumper Size 18 (033)
Very nice ladies jumper. Auctiva's FREE Counter. Armpit to armpit 21". Length 25".
Ladies Black & White Round Neck Jumper Pullover Sz 12 M Papaya Essentials
BNWT Ladies 20-22 Brown Multi Longer Length Jumper
BNWT Ladies Size 20-22 XL) brown multi Longer Length jumper Perfect smart casual wear From a smoke free home Thanks for looking
Next Women's None Cotton Jumpers & Cardigans
Topshop Cotton Jumpers without Fastening for Women
Warehouse Women's None Cotton Jumpers & Cardigans


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BBC - Radio 1 Movies Blog: Edith meets George Clooney and Renee Zellweger
Edith meets George Clooney and Renee Zellweger
Edith Bowman | 15:41 UK time, Tuesday, 10 February 2009
(Originally published on 10 April 2008)
Here's the full video of Edith chatting to George Clooney and Renee Zellweger, starting with the bits before the interview started, and including some extra bits we didn't have time for on the radio, and the much talked about Clooney Cuddle!

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Dairy Queen, Omaha - 404 N 114th St - Restaurant Reviews & Phone Number - TripAdvisor
#39 of 42 Dessert in Omaha#1,052 of 1,199 Places to Eat in Omaha}
404 N 114th St, Omaha, NE 68154-2519
+1 402-333-4623
Disorganized and extremely slow. Gross eating area. Never again. How can other restaurants get food out in a timely manner, and this place can't. Terrible terrible terrible
I went to DQ on 114 andDodge with my daughter to have a special date night . The front help was friendly . However the restaurant it self was absolutely disgusting ..I could see all the mess behind the counters and the excess drippings on...More
phoffman2017
In the last month we have gone to this DQ several times trying to give it another chance and every time it's a disaster. The wait is extremely long, the order girl/cashier is unhappy and down right nasty. At 3 in the afternoon, there's no...More
Thank phoffman2017
Ice Cream with the Grandson
My grandson and I stopped at this DQ for ice cream -- not a novel idea. It wasn't busy -- we were one of two in store customers. It was hot outside; hot and muggy inside. It looked like the drink stand sprung a leak...More
Nice place for a quick meal or treat
They have not only pretty good treats but also decent hot food too. I had a half pound double cheeseburger meal. The burger was better then most other fast food places and my fries were great. Then, to top it off, if you are not...More
The store is a little older, but the food was good and the staff was super efficient. They were friendly and got our food out quickly. It was pretty crowded after two softball teams came in during our meal, but the staff handled it all...More
Nothing Beats DQ for Soft Serve
Each day, traveling to and from work, I pass the DQ at 72nd and Blondo, and the voice of temptation begins to work on me, for there is nothing better than DQ soft serve, especially when it's chocolate and blended into a malt. I have...More
This establishment is also just around the corner from my house, and I decided to stop & eat on the way home. Now, I've eaten there before, and the food is always good, and the service is fast. It brings back memories of when I...More
Address: 404 N 114th St, Omaha, NE 68154-2519
Phone Number: +1 402-333-4623

value: This website is an American online travel company that operates a website and mobile app with user-generated content and a comparison shopping website.


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Friend, I Always Thank My God For You Mug
$16.92 Retail: $36.99 Save 54% ($20.07)
Stock No: WW113408
Friend, A Sweet Friendship Refreshes Your Soul Word Figure
I Wouldn't Be Me If I Didn't Have You As A Friend Angel Holding Flowers
A True Friend is a Treasure of the Heart, Yellow Jar with Flowers
Friends Are A Gift from God Plaque
A Prayer For My Friend, Framed Art

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This website is a Christian catalog and internet retailer.
Christian Book Distributors was started in 1978 by Stephen Hendrickson, 19 years old and a sophomore at Central Bible College in Springfield, Missouri, out of his parents’ home in Lynn, Massachusetts.