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Patent US4001140 - Capsule manufacture - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsA process is disclosed for performing encapsulation, en masse, by an in situ polymerization reaction to yield capsule wall material. The polymerization includes a reaction between urea and formaldehyde in an aqueous vehicle and the reaction is conducted in the presence of negatively-charged, carboxyl-substituted,...http://www.google.com/patents/US4001140?utm_source=gb-gplus-sharePatent US4001140 - Capsule manufactureAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS4001140 APublication typeGrantApplication numberUS 05/487,322Publication dateJan 4, 1977Filing dateJul 10, 1974Priority dateJul 10, 1974Also published asCA1048867A, CA1048867A1, DE2529427A1, DE2529427B2, DE2529427C3, US4087376, US4089802Publication number05487322, 487322, US 4001140 A, US 4001140A, US-A-4001140, US4001140 A, US4001140AInventorsPeter L. Foris, Robert W. Brown, Paul S. Phillips, Jr.Original AssigneeNcr CorporationExport CitationBiBTeX, EndNote, RefManPatent Citations (4), Referenced by (341), Classifications (17), Legal Events (2) External Links: USPTO, USPTO Assignment, EspacenetCapsule manufacture
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2013 St. George Illawarra Dragons season - Wikipedia
2013 St. George Illawarra Dragons season
Wins: 7; Losses: 17
For: 379; Against: 530
WIN Jubilee Oval WIN Stadium
9 Daniel Vidot, Jason Nightingale & Brett Morris
21 Jamie Soward
58 Jamie Soward
The 2013 St. George Illawarra Dragons season was the 15th in the joint venture club's history. Coached by Steve Price and captained by Ben Creagh, they competed in the National Rugby League's 2013 Telstra Premiership. This season was the worst year performance wise since the joint venture was created. They finished only over the top of Wests Tigers & the Parramatta Eels.
The Dragons started the season losing their first 3 games to Melbourne Storm, Brisbane Broncos & Canberra Raiders only to come back and win their next 3 over Cronulla Sharks, Newcastle Knights & Wests Tigers. The Knights win coming at a cost, with the injury of New Zealand international Gerard Beale for the season. Unfortunately for the Dragons those were the only wins they could string together in 2013 losing another 3 to the Sydney Roosters, the Manly-Warringah Sea Eagles and the Gold Coast Titans.
Round 10 saw a glimmering light at the end of the tunnel for the Dragons when they signed former Canberra Raiders superstar Josh Dugan. Dugan was seen as the answer to the fullback spot left by the injured Beale. He kicked off his career at the joint venture scoring 2 tries in the team's romp of the Parramatta Eels 32-12 at WIN Stadium.
The next week the Dragons were sent back to earth with a thud as they became the first St. George Dragons / St. George Illawarra Dragons team to be kept to nil at Jubilee Oval as the Penrith Panthers ran in 3 unanswered tries to win 19-0. This was followed by a loss to the Canterbury-Bankstown Bulldogs by 2 points. This was the last game for the club's all time leading point scorer Jamie Soward for the Dragons as he was dumped out of the side and two weeks later released to join the London Broncos of the Super League.
Over the month of June the Dragons 3-1 record with only a solitary win of the Newcastle Knights in Newcastle, Josh Dugan again scoring 2 tries.
July started disastrously for the Red V again being kept to nil by the Sydney Roosters, losing 36-0. After this night the Dragons had to win almost every remaining game to stay in the final hunt. Round 17 saw first v 2nd last as the Dragons played the South Sydney Rabbitohs away at ANZ Stadium with the Dragons showing true grit to score twice in 10 mins to bring the game into extra time. Brett Morris getting the winning try in golden point extra time.
However the Dragons then went on a streak that was etched in the club's history books, losing their next 6 games, including the 2 teams below them on the points table, an embarrassing loss to Wests Tigers and the Parramatta Eels. Four of those six games were lost by 4 points or less, including a heart-stopping loss to the Cronulla-Sutherland Sharks where Sharks player Andrew Fifita scored on the buzzer to win the local derby after trailing the whole game.
In the last game of the year the Red V said goodbye to some legends of the game including Matt Cooper (rugby league), Nathan Fien and Michael Weyman. The 17 on the field, scoring an impressive 19-10 win over the New Zealand Warriors who needed a win to keep their finals hopes alive.
Leeson Ah Mau - PR, SR
Matt Cooper - CE
Ben Creagh (c) - SR
Evander Cummins - FB
Jack De Belin - PR, SR
Nathan Fien (vc) - HK
Bronx Goodwin - FB
Gerard Beale - FB, CE
Nathan Green - CE
Damian Cook - SR
Tyson Frizell - SR, LK
Josh Drinkwater - HB
Jake Marketo - SR, LK
Will Matthews - SR
Trent Merrin - PR
Adam Quinlan - FB, FE
Brett Morris - FB, WG
Jason Nightingale - FB, WG
Matt Prior - PR, SR
Charly Runciman - CE
Jamie Soward (gk) - FE
Chase Stanley - CE
Kyle Stanley - FB, FE
Atelea Vea - SR
Daniel Vidot - CE, WG
Michael Weyman - CE
Craig Garvey - HK
Josh Drinkwater from Manly-Warringah Sea Eagles
Gerard Beale from Brisbane Broncos
Bronson Harrison from Canberra Raiders
Taioalo Vaivai from Parramatta Eels
Tyson Frizell from Cronulla-Sutherland Sharks
Harry Siejka from New Zealand Warriors
Josh Dugan Free Agent from Round 10
Jack Buchanan to Wests Tigers
David Gower to Manly-Warringah Sea Eagles
Ben Hornby Retired
Denan Kemp to Brisbane Broncos
Jeremy Latimore to Penrith Panthers
Jake Marketo to Redcliffe Dolphins
Josh Miller Retired
Dean Young Retired
Beau Scott to Newcastle Knights
Jamie Soward Released from Round 12
Retrieved from "https://en.wikipedia.org/w/index.php?title=2013_St._George_Illawarra_Dragons_season&oldid=906738191"
St. George Illawarra Dragons seasons
This page was last edited on 17 July 2019, at 21:54 (UTC).

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The rooms were more than adequate, it was like... - Review of 3rd Coast Inn - TripAdvisor
The rooms were more than adequate, it was like... - Review of 3rd Coast Inn
“Family friendly envelopment”
“This place is great! It is so clean and...”
“We reserved a room based on that rating”
“Once the sun set, the pool and hot tub area came...”
“Having in the past had my own amazing hot tub, I...”
“The 3rd Coast Inn is Pet friendly and that was a...”
“We stayed in Room No 5 and we had such a great...”
“Close to the beach and next door to a Stripes, it...”
“Literally less than half a mile from the beach...”
“The pool and hot tub were wonderful and very clean”
“This was our 3rd time staying at 3rd Coast Inn...”
See the most recent reviews E6Plumbing
1 review 1 helpful vote “Best place on the island!”
I first want to say thank you for the most amazing experience I have had in port aransas in 15 years. My most favorite thing were the bicycles. Me and my 5 year old son rode bikes all day for two days. They had bikes for all ages and we tried every single one to find our perfect one. The rooms were more than adequate, it was like having all the amenities of home. We cooked a wonderful breakfast and went out to the pool. We then cooked a wonderful burger and hot dog lunch for the kids on their BBQ pit by the pool. The kids swam all day day and the adults hung in the hot tub. The staff was amazing, so sweet and helpful. They had movies in the office for the kids and games for the adults. Just he most amazing trip ever. I would recommend it to anyone anytime We also met some dear friends that were our neighbors and I believe we will be friends for life ! Thanks again for our wonderful time I can't wait to come back!
Thank E6Plumbing Report Ask E6Plumbing about 3rd Coast Inn
English first Any Flowers2by2
Level Contributor 5 reviews 2 helpful votes “1st Anniversary”
When we got married we couldn't think of a better place to stay than the 3rd Coast Inn so we took our chances and drove down. Much to our surprise we had the whole place to ourselves. What an awesome week that was 4/2/12-4/8/12. So when our 1 yr anniversary rolled around we thought maybe Vegas or some exotic island getaway. But one evening while brainstorming with my husband we looked at each other and our eyes lit up and together we said 3rd Coast Inn! Once again we had to time of our lives. We just can't say enough about the cleanliness of the rooms, the hospitality of Darcy, Deanna and the family or the new mattress toppers in the rooms. We slept like babies, partied like college kids and fished to our hearts content (my husband's heart that is!) This is and will always be one of my most favorite places in Texas and it is a big state. So till next time 3rd Coast Inn...Thanks and we miss you!
Room Tip: If you have a family with a couple of little ones, room 5 has a bunk bed and a queen size bed with a...
Helpful? Thank Flowers2by2 Report Ask Flowers2by2 about 3rd Coast Inn
67 helpful votes “Perfect place for a perfect weekend”
This is exactly what we needed. Clean friendly and close to everything!!! You can walk or bike to anywhere in Port A from here (oh and use of the bikes are free). We loved our little rooms. The decoration is be cute and the accommodation is very clean. Our unit had two beds and a full kitchen, which we used to make breakfast every morning. This is a perfect place to wind down and chill out. It is not high end, nor does it pretend to be but what it lacks in thread count it more than makes up in charm and hospitality. Every night after dinner we went to the hot tub to relax or sat in the covered area with a fire to keep us warm. One night we were on our own and another we sat with other residents. There are BBQ if you want to grill your dinner and a lovely big hammock for a nap during the day. We are already talking about when we are going to head down to Port A again, and when we do we will stay at 3rd Coast Inn.
Thank sgeddie Report Ask sgeddie about 3rd Coast Inn
We stayed one night after doing the boat trip out of Fulton Harbor to see the whooping cranes. (We loved the boat trip!) The staff was friendly, but the room was not really what we expected. The door opened onto what was the grassy parking lot and every time we walked in the room, we brought the dust and grass with us, despite the fact that we moved a little rug from in front of the bed and used it to wipe our feet. It was noisy from the people next door - we could hear all their talking and their TV. The room was quite small, you could barely walk around it without bumping into the little table that the micro and coffee maker were on. The mattress was the kind where you kept rolling into the middle. They tried to brighten it up by painting the concrete a bright blue and red. The bedspread was black. There were newspaper pictures of pirates taped to the walls (I think it was the 'theme' of the pirate room). It was clean. It was adequate. But if we ever go back, and we might because the birds here are fantastic!, we would try to find another place to stay.
Thank TravelinGal1029 Report Ask TravelinGal1029 about 3rd Coast Inn
1 review 3 helpful votes “Comfortable, quiet, and immaculately clean - love this place!”
We spent a couple of days in Port Aransas and stayed here, we loved it and are planning a return stay soon! Small and not showy from the street view, this is a hidden gem. Staff are super friendly, helpful and kind - full of tips on where to eat, shop, sightsee, etc. Our room was immaculately clean - (as a nurse, I really care about this!) - comfortable, and super cute with seashore themed decor and bright colors - love it! They have a spacious pool and hottub also, we were pretty tired and didn't try that out, but maybe next time. Looking forward to our next trip down there - very soon!
Thank SkyRN Report Ask SkyRN about 3rd Coast Inn
31 helpful votes “Wonderful place to relax”
We spent two relaxingly nice nights here on our weeklong Texas tour trip. Small, quiet place with the friendliest folks you could ever want to get to know. Our room (#7) was adjacent to office and had it's very own porch area, that others came over and used with us just to sit and visit. It was windy as all get out while we were in Port A, so we did not go to beach (would have been sandblasted!) so we spent time just exploring and relaxing at the Inn. Highly recommended get away. Forget goint to national chain hotels and take time to relax and enjoy the simple life here!
Room Tip: Room #7 was small with huge bathroom. And had an area to sit out in the morning to have coffee, very...
Thank councilman05 Report Ask councilman05 about 3rd Coast Inn
Thanks for the wonderful review! We are so happy that you enjoyed your time spent with us, and hope you are able to visit us again in the future!
What really makes this place stand out is what you experience while staying here. From outside appearances at the street, it's hard to tell that this place is a gem in disguise. Once you're on the grounds and can see the pool and the colorful flowers, you begin to realize that it's not another big-box hotel or a sleazy motel. Then as you pass by the owners and exchange small talk, you realize that the owners genuinely enjoy what they do. They work hard, bring a nice presence with them, and do the best they can. The room my wife and I stayed at was connected to the office. To a person with high expectations for a fancy room, you would find yourself being unsatisfied. But to someone who just wants to get away from the world and relax, the room and the nearby sitting area outside is perfect. Glad that we found this place.Tip - If you're looking to be directly on the beach, this isn't the right place. But believe me, the pool and this location in general is a fine getaway from the beach and in the night, I'd say the pool is far better than being out on the beach.
Room Tip: The room connected to the office is nice because of the adjoining outdoor sitting area with laid bac...
Thank Joe30196 Report Ask Joe30196 about 3rd Coast Inn
We are so glad that you enjoyed your time spent with us, and glad that you enjoyed our plants! We hope that you will be able to come see us again soon.
Explore similar hotels BEST WESTERN Port Aransas 772 ReviewsShow Prices 0.0 miles Island Hotel Port Aransas 428 ReviewsShow Prices 1.2 miles Plantation Suites 333 ReviewsShow Prices 0.8 miles See all Port Aransas hotels Browse nearby

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Patent US5278345 - Use of ECR-1, mazmorite and ECR-18 in the conversion of oxygenates to ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsA process for converting an oxygenate feed to light olefins using a microporous zeolite catalysts such as ECR-1, mazmorites, and/or ECR-18 is provided. In a preferred embodiment, the process is conducted at approximately 440� C. (824� F.) to about 460� C. (860� F.) using a methanol:water (approximately...http://www.google.com/patents/US5278345?utm_source=gb-gplus-sharePatent US5278345 - Use of ECR-1, mazmorite and ECR-18 in the conversion of oxygenates to hydrocarbonsAdvanced Patent SearchPublication numberUS5278345 APublication typeGrantApplication numberUS 07/888,668Publication dateJan 11, 1994Filing dateMay 27, 1992Priority dateMay 27, 1992Fee statusPaidAlso published asCA2136627A1, CA2136627C, DE69308305D1, DE69308305T2, EP0649394A1, EP0649394B1, WO1993024429A1Publication number07888668, 888668, US 5278345 A, US 5278345A, US-A-5278345, US5278345 A, US5278345AInventorsMarcel J. G. Janssen, David E. W. VaughanOriginal AssigneeExxon Chemical Patents Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (18), Non-Patent Citations (4), Referenced by (21), Classifications (12), Legal Events (4) External Links: USPTO, USPTO Assignment, EspacenetUse of ECR-1, mazmorite and ECR-18 in the conversion of oxygenates to hydrocarbons
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS3928483 *Sep 23, 1974Dec 23, 1975Mobil Oil CorpProduction of gasoline hydrocarbonsUS4025575 *Apr 8, 1975May 24, 1977Mobil Oil CorporationProcess for manufacturing olefinsUS4401637 *Feb 17, 1981Aug 30, 1983Basf AktiengesellschaftCrystalline isotactic zeolites and their preparationUS4423273 *Jul 22, 1982Dec 27, 1983Basf AktiengesellschaftPreparation of olefins from methaol and/or dimethyl etherUS4447669 *Dec 17, 1982May 8, 1984Societe Chimique De La Grande Paroisse, Azote Et Produits ChimiquesProduction of hydrocarbons from methanol in the presence of zeolite catalystsUS4467133 *Jun 21, 1982Aug 21, 1984Mobil Oil CorporationConversion of alcohols and ethers to distillate range hydrocarbonsUS4496786 *Sep 30, 1983Jan 29, 1985Chevron Research CompanySelective conversion of methanol to low molecular weight olefins over high silica SSZ-13 zeoliteUS4499314 *Apr 30, 1984Feb 12, 1985Imperial Chemical Industries PlcMethanol conversion to hydrocarbons with zeolites and cocatalystsUS4544793 *Nov 18, 1983Oct 1, 1985Director-General Of Agency Of Industrial Science And TechnologyCrystalline zeolite, method of preparing same and process for the production of olefins using same as catalystUS4547616 *Dec 28, 1984Oct 15, 1985Mobil Oil CorporationConversion of oxygenates to lower olefins in a turbulent fluidized catalyst bedUS4579994 *Feb 28, 1985Apr 1, 1986Itaru TodorikiProcess for preparing lower olefin using calcium phosphate modified zeolite type catalystUS4657748 *Mar 18, 1985Apr 14, 1987Exxon Research And Engineering CompanyCrystalline zeolite (ECR-1) and process for preparing itUS4661332 *Jul 29, 1985Apr 28, 1987Exxon Research And Engineering CompanyZeolite (ECR-18) isostructural with paulingite and a method for its preparationUS4677243 *Feb 11, 1985Jun 30, 1987Union Carbide CorporationProduction of light olefins from aliphatic hetero compoundsUS4698449 *Jul 31, 1986Oct 6, 1987Research Association For Petroleum Alternatives DevelopmentCrystalline silicates, and processes for the production or use thereofUS4843183 *Jul 20, 1987Jun 27, 1989Showa Shell Sekiyu Kabushiki KaishaProcess for producing lower olefinic hydrocarbons from methanolUS4892721 *Apr 2, 1987Jan 9, 1990Exxon Research And Engineering CompanyCrystalline zeolite materials constituting maz-morite intermediatesUS5013536 *Aug 22, 1988May 7, 1991Exxon Research And Engineering CompanyECR-18, method of its preparation, and uses for sorption and separation* Cited by examinerNon-Patent CitationsReference1Vaughan, D. E. W. et al., "Characterization of the New Zeolite ECR-1," in Zeolite Synthesis, (Ocelli, M. L. et al. eds, ACS Press, 1989).2Vaughan, D. E. W. et al., "Synthesis of the New Large-Pore Zeolite ECR-1," in Zeolite Synthesis (Ocelli, M. L. et al. eds, ACS Press, 1989).3 *Vaughan, D. E. W. et al., Characterization of the New Zeolite ECR 1, in Zeolite Synthesis, (Ocelli, M. L. et al. eds, ACS Press, 1989).4 *Vaughan, D. E. W. et al., Synthesis of the New Large Pore Zeolite ECR 1, in Zeolite Synthesis (Ocelli, M. L. et al. eds, ACS Press, 1989).* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS6046372 *Apr 6, 1998Apr 4, 2000Mobil Oil CorporationProcess for producing light olefinsUS6436869Mar 2, 2000Aug 20, 2002Exxonmobil Chemical Patents Inc.Iron, cobalt and/or nickel containing ALPO bound SAPO molecular sieve catalyst for producing olefinsUS6441261Jul 28, 2000Aug 27, 2002Exxonmobil Chemical Patents Inc.High pressure oxygenate conversion process via diluent co-feedUS6444868Mar 8, 2000Sep 3, 2002Exxon Mobil Chemical Patents Inc.Process to control conversion of C4+ and heavier stream to lighter products in oxygenate conversion reactionsUS6486219Sep 27, 2000Nov 26, 2002Exxonmobil Chemical Patents, Inc.Methanol, olefin, and hydrocarbon synthesis processUS6506954Oct 13, 2000Jan 14, 2003Exxon Mobil Chemical Patents, Inc.Process for producing chemicals from oxygenateUS6514899Oct 2, 2000Feb 4, 2003Exxonmobil Chemical Patents, Inc.Process for the synthesis of silicoaluminophosphate molecular sievesUS6531639Feb 18, 2000Mar 11, 2003Exxonmobil Chemical Patents, Inc.Catalytic production of olefins at high methanol partial pressuresUS6613950Jun 6, 2000Sep 2, 2003Exxonmobil Chemical Patents Inc.Stripping hydrocarbon in an oxygenate conversion processUS6613951Sep 23, 1999Sep 2, 2003Mobil Oil CorporationProcess for converting methanol to olefinsUS6743747Feb 24, 2000Jun 1, 2004Exxonmobil Chemical Patents Inc.Catalyst pretreatment in an oxgenate to olefins reaction systemUS6768034Jan 9, 2003Jul 27, 2004Exxonmobil Chemical Patents Inc.Catalytic production of olefins at high methanol partial pressuresUS7083762Jan 8, 2003Aug 1, 2006Exxonmobil Chemical Patents Inc.Multiple riser reactor with centralized catalyst returnUS7102050May 4, 2000Sep 5, 2006Exxonmobil Chemical Patents Inc.Multiple riser reactorUS7102051Aug 3, 2004Sep 5, 2006Exxonmobil Chemical Patents Inc.Treatment of molecular sieves with silicon containing compoundsUS7122160Sep 24, 2002Oct 17, 2006Exxonmobil Chemical Patents Inc.Reactor with multiple risers and consolidated transportUS7195741Mar 9, 2005Mar 27, 2007Exxonmobil Chemical Patents Inc.Multiple riser reactorUS7199277Jul 1, 2004Apr 3, 2007Exxonmobil Chemical Patents Inc.Pretreating a catalyst containing molecular sieve and active metal oxideUS7385099Jun 14, 2006Jun 10, 2008Exxonmobil Chemical Patents Inc.Multiple riser reactor with centralized catalyst returnUS8053070Mar 5, 2008Nov 8, 2011Samsung Electronics Co., Ltd.Optical film having graded refractive index and method of manufacturing the sameWO1999051548A1 *Apr 1, 1999Oct 14, 1999Mobil Oil CorpProcess for producing light olefins* Cited by examinerClassifications U.S. Classification585/640, 585/639, 585/638International ClassificationC07C11/02, C07B61/00, C07C1/20, C07C1/32, B01J29/80, C07C1/26Cooperative ClassificationC07C2529/70, C07C1/20European ClassificationC07C1/20Legal EventsDateCodeEventDescriptionJun 30, 2005FPAYFee paymentYear of fee payment: 12Jun 29, 2001FPAYFee paymentYear of fee payment: 8Jun 30, 1997FPAYFee paymentYear of fee payment: 4Nov 9, 1992ASAssignmentOwner name: EXXON CHEMICAL PATENTS INC., DELAWAREFree format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:JANSSEN, MARCEL J. G.;VAUGHAN, DAVID E. W.;REEL/FRAME:006290/0689Effective date: 19921103RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services


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Effect of acute exercise on AMPK signaling in skeletal muscle of su... - PubMed - NCBI
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See comment in PubMed Commons belowDiabetes. 2007 Mar;56(3):836-48.Effect of acute exercise on AMPK signaling in skeletal muscle of subjects with type 2 diabetes: a time-course and dose-response study.Sriwijitkamol A1, Coletta DK, Wajcberg E, Balbontin GB, Reyna SM, Barrientes J, Eagan PA, Jenkinson CP, Cersosimo E, DeFronzo RA, Sakamoto K, Musi N.Author information1Texas Diabetes Institute, and Diabetes Division, University of Texas Health Science Center at San Antonio, TX 78207, USA.AbstractActivation of AMP-activated protein kinase (AMPK) by exercise induces several cellular processes in muscle. Exercise activation of AMPK is unaffected in lean (BMI approximately 25 kg/m(2)) subjects with type 2 diabetes. However, most type 2 diabetic subjects are obese (BMI >30 kg/m(2)), and exercise stimulation of AMPK is blunted in obese rodents. We examined whether obese type 2 diabetic subjects have impaired exercise stimulation of AMPK, at different signaling levels, spanning from the upstream kinase, LKB1, to the putative AMPK targets, AS160 and peroxisome proliferator-activated receptor coactivator (PGC)-1alpha, involved in glucose transport regulation and mitochondrial biogenesis, respectively. Twelve type 2 diabetic, eight obese, and eight lean subjects exercised on a cycle ergometer for 40 min. Muscle biopsies were done before, during, and after exercise. Subjects underwent this protocol on two occasions, at low (50% Vo(2max)) and moderate (70% Vo(2max)) intensities, with a 4-6 week interval. Exercise had no effect on LKB1 activity. Exercise had a time- and intensity-dependent effect to increase AMPK activity and AS160 phosphorylation. Obese and type 2 diabetic subjects had attenuated exercise-stimulated AMPK activity and AS160 phosphorylation. Type 2 diabetic subjects had reduced basal PGC-1 gene expression but normal exercise-induced increases in PGC-1 expression. Our findings suggest that obese type 2 diabetic subjects may need to exercise at higher intensity to stimulate the AMPK-AS160 axis to the same level as lean subjects.PMID: 17327455 [PubMed - indexed for MEDLINE] PMCID: PMC2844111 Free PMC ArticleShareImages from this publication.See all images (7)Free textFIG. 1Basal AMPK, ACC, AS160, and Akt. AMPK subunit, ACC, AS160, and Akt protein content (A) and phosphorylation (B) were measured in 8 lean (□), 8 obese (▥), and 12 type 2 diabetic (T2DM) (■) subjects. Data are means ± SE. Blots are shown for three subjects/group.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 2Effect of exercise on AMPK and ACC phosphorylation. Biopsies were done at basal (□), after 10 (▥) and 40 min (■) of exercise, and 150 min postexercise (▨). Data are means ± SE in 8 lean, 8 obese, and 12 type 2 diabetic (T2DM) subjects. Data are expressed as arbitrary units (A and C) and as fold change (B and D). *P < 0.05 vs. basal of respective group; †P < 0.05 vs. lean group at 40 min. Blots are shown for one subject/group. B, basal; R, rest postexercise.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 3AMPK activity. AMPKα1, AMPKα2, and total AMPK activities were measured before (□), after 40 min of exercise (■), and 150 min postexercise (▨). Data are means ± SE. Data are expressed as kinase activity (A, C, and E) and as fold change (B, D, and F). n = 6–12 in each time point (samples were not available for all the assays). *P < 0.05 vs. basal of respective group; †P < 0.05 vs. lean group in respective time point. T2DM, type 2 diabetes.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 4LKB1 expression and activity. Equal amounts of protein (40 μg) were used for blotting of LKB1 and MO25α (A). Blots are shown for two subjects per group. LKB1 activity was measured as described in research design and methods (B). B, basal; T2DM, type 2 diabetes.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 5AS160 phosphorylation. Immunoblots are shown for two subjects in the basal state and after 40 min of exercise, after immunoprecipitation with AS160 and probing with PAS antibody (A). Biopsies were done at basal (□), after 10 (▥) and 40 min (■) of exercise, and 150 min postexercise (▨). Data are means ± SE in 8 lean, 8 obese, and 12 type 2 diabetic (T2DM) subjects. Data are expressed as arbitrary units (B) and as fold change (C). *P < 0.05 vs. basal of respective group; †P < 0.05 vs. lean at 150 min postexercise. Blots are shown for one subject/group. B, basal; R, rest postexercise.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 6Akt phosphorylation. Biopsies were done at basal (□), after 10 (▥) and 40 min (■) of exercise, and 150 min postexercise (▨). Akt-Ser473 (A) and Thr308 (B) were measured as described in research design and methods. Data are means ± SE in 8 lean, 8 obese, and 12 type 2 diabetic (T2DM) subjects. *P < 0.05 vs. basal of respective group. Blots are shown for one subject/group. B, basal; R, rest postexercise.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.FIG. 7PGC-1 and NRF-1 gene expression. Basal PGC-1α (A) and NRF-1 (C) gene expression. Effect of exercise on PGC-1 (B) and NRF-1 (D) gene expression. Gene expression was measured at baseline, after 40 min of exercise, and 150 min postexercise. Data are means ± SE in 8 lean, 8 obese, and 12 type 2 diabetic (T2DM) subjects. *P < 0.05 vs. type 2 diabetes group; †P < 0.05 vs. basal of respective group; ‡P = 0.05 vs. basal in obese group.Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 DiabetesDiabetes. ;56(3):836-848.Publication Types, MeSH Terms, Substances, Grant SupportPublication TypesClinical TrialResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.MeSH TermsAMP-Activated Protein KinasesAdultAmino Acid Oxidoreductases/metabolismBlood Glucose/metabolismDiabetes Mellitus, Type 2/metabolism*Diabetes Mellitus, Type 2/physiopathologyExercise/physiology*FemaleGTPase-Activating Proteins/metabolismGlycogen/metabolismHumansMaleMiddle AgedMultienzyme Complexes/metabolism*Muscle, Skeletal/metabolism*Nuclear Respiratory Factor 1/metabolismNucleotides/metabolismObesity/metabolismObesity/physiopathologyPhosphorylationProtein-Serine-Threonine Kinases/metabolism*Proto-Oncogene Proteins c-akt/metabolismSignal Transduction*Time FactorsTranscription Factors/metabolismSubstancesBlood GlucoseGTPase-Activating ProteinsMultienzyme ComplexesNRF1 protein, humanNuclear Respiratory Factor 1NucleotidesTBC1D4 protein, humanTranscription Factorsperoxisome-proliferator-activated receptor-gamma coactivator-1GlycogenAmino Acid Oxidoreductases1-aminocyclopropane-1-carboxylic acid oxidaseSTK11 protein, humanAMP-Activated Protein KinasesProtein-Serine-Threonine KinasesProto-Oncogene Proteins c-aktGrant SupportDK 067690/DK/NIDDK NIH HHS/United StatesDK 24092/DK/NIDDK NIH HHS/United StatesF32 HL086089/HL/NHLBI NIH HHS/United StatesF32 HL086089-03/HL/NHLBI NIH HHS/United StatesL60 MD000986/MD/NIMHD NIH HHS/United StatesL60 MD000986-03A2/MD/NIMHD NIH HHS/United StatesMC_U127088492/Medical Research Council/United KingdomR01 DK024092/DK/NIDDK NIH HHS/United StatesR01 DK024092-27/DK/NIDDK NIH HHS/United StatesR01 DK067690/DK/NIDDK NIH HHS/United StatesR01 DK067690-05/DK/NIDDK NIH HHS/United StatesR01 DK079195/DK/NIDDK NIH HHS/United StatesLinkOut - more resourcesFull Text SourcesHighWireEBSCOEurope PubMed Central - Author ManuscriptOvid Technologies, Inc.ProQuestPubMed Central - Author ManuscriptPubMed Central Canada - Author ManuscriptOther Literature SourcesCOS Scholar UniverseLabome Researcher Resource - ExactAntigen/LabomeAccess more work from the authors - ResearchGateMedicalDiabetes - Genetic AllianceDiabetes, Type 2 - Genetic AllianceDiabetes Type 2 - MedlinePlus Health InformationExercise and Physical Fitness - MedlinePlus Health InformationPubMed Commons home

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