text:
Patent US5919712 - Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsMethods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor...http://www.google.com/patents/US5919712?utm_source=gb-gplus-sharePatent US5919712 - Apparatus and methods for multi-analyte homogeneous fluoro-immunoassaysAdvanced Patent SearchPublication numberUS5919712 APublication typeGrantApplication numberUS 08/748,687Publication dateJul 6, 1999Filing dateNov 13, 1996Priority dateMay 18, 1993Fee statusPaidAlso published asUS6316274, US6979567, US20020160535Publication number08748687, 748687, US 5919712 A, US 5919712A, US-A-5919712, US5919712 A, US5919712AInventorsJames N. Herron, Douglas A. Christensen, Hsu-Kun Wang, Karin D. Caldwell, Vera Janatova, Shao-Chie HuangOriginal AssigneeUniversity Of Utah Research FoundationExport CitationBiBTeX, EndNote, RefManPatent Citations (51), Non-Patent Citations (42), Referenced by (110), Classifications (51), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetApparatus and methods for multi-analyte homogeneous fluoro-immunoassays
The embodiment of FIG. 1 further includes a 45� angle mirror 110 which is positioned for making beam 102 a vertical beam prior to focussing the beam onto the biosensor. It will be understood by those skilled that the number and arrangement of mirrors 104, 106, 108, 110 may be varied as necessary to accommodate various space limitations, with the sole requirement being that a sufficient amount of light be directed to biosensor 120.
Of further interest in FIG. 4A is the orientation of lenses 404, 406 with respect to waveguide 302. It will be seen that the curved edges 404A, 406A face towards waveguide 302, which is 180� from the orientation depicted in FIG. 3C. While the focussing lens can be oriented in either way, the arrangement of FIG. 4A is presently preferred for illumination of the waveguide with the flow-through cell.
In a further and highly preferred embodiment of the biosensor depicted in FIGS. 12A and 12B, the end of the planar waveguide which is distal to the receiving edge, has a portion 650 of serrated or toothed shape. The angles 652A, 652B must be less than the critical angle for total internal reflection at the waveguide-air interface (the critical angle for polystyrene/air is about 51�). Preferably, the sum of angles 652A, 652B should be 90�, so that the light is retro-reflected back along the longitudinal axis of the waveguide; still more preferably, angle 652A=652B=45�. An advantage of this shape is that it increases the level of internal reflection without a reflective coating on the edges, thus reducing manufacturing complexity and costs.
FIG. 19 shows data for the fluorescence intensity of biotin-conjugated Cy5 dye bound to an avidin-coated silica waveguide (ch. A) as a function of beam entry angle, as compared to the "background" level of fluorescence from Cy5 dye free in solution (not conjugated to biotin and therefore not bound to the avidin-coated surface). The critical angle for TIR at the silica-aqueous solution boundary is about 26�. The data of FIG. 19 indicate that the signal-to-background ratio (ch.A�ch.B) for a beam entry angle of 20� to 25� is about 4-fold higher than for 0�. Thus, beam entry angles of one to about five degrees less than the critical angle are presently preferred for silica waveguides. For a polystyrene waveguide with an aqueous adjacent medium, the critical angle for TIR is about 33�, and a useful range of beam entry angles is from about 25� to about 32�, with the higher angles generally being preferable.
Silica chips or glass or quartz microscope slides were cleaned with chromic acid, then treated with 5% APS/95% deionized water (v/v) for about fifteen minutes at room temperature. The APS-treated surfaces were rinsed with deoionized water and absolute ethanol, and incubated in a vacuum oven which had been flushed at least three times with nitrogen, at 120� C. for 1 hour. The resulting silanized surfaces were then soaked in 2.5% glutaraldehyde (E.M. grade from Polysciences) in 0.1M carbonate-bicarbonate buffer, pH 9.2, for two hours at room temperature.
Next, linear PMahy was reacted with the aldehyde groups on the treated chips to create a cross-linked polymer film with many unreacted hydrazido groups in the chains. This was done by dipping the treated chips in solutions of PMahy of between about 5% and 8% (w/v), pH 5.2, at a temperature between about room temperature and about 60� C., for a time sufficient to form a polymer film of thickness about 100 Å or less. The thickness of the hydrogel layer increases with time and temperature of incubation in the solution. It was found that optimal conditions for preparation of the film of 100 Å thickness or less, comprised incubating in 5% (w/v) PMahy for 2 hours at room temperature (about 25� C.).
Following derivatization with SMCC, the hydrogel-coated surfaces were treated with a 1 mg/ml solution of Fab' fragments in phosphate buffer, ph 6.0, with 5 mM EDTA. The waveguide surface so prepared was shown to immobilize Fab' molecules at a surface density of about 1.4�10-12 moles/cm2. Also the surface was able to immobilize Fab' fragments at their C-terminal thiol groups in a site-specific way. The thickness of the resulting polymer film was determined by ellipsometry to be about 100 Å, as was desired. This film thickness is much less than typical previous polymeric films, which have thicknesses of 0.35 to 25 μm (microns). The above-described method of preparing the PMahy polymers is superior to that described by von Kern et al. using polymethacryloylacid esters. Such esters suitable for reaction with hydrazine hydrate often have a molecular weight of 80,000 daltons or more, from which it is difficult to obtain a desirably thin film on the waveguide.
In actual experiments, the procedure was as follows. Chromic acid-cleaned silica surfaces were immersed in a solution of 3�10-6 M (molar) avidin for about 3 hours at room temperature. The surfaces were then washed several times in PBS to remove unadsorbed avidin.
An alternate method was used for biotinylating whole antibodies, in which biotin-LC-hydrazide was coupled to oxidized carbohydrate groups in the Fc region of the antibody. Mab designated 9-40 (a murine monoclonal IgG1 antibody that binds fluorescein), was oxidized by incubation at a concentration of 1-2 mg/ml protein in 10 mM sodium periodate, 0.1 M sodium acetate pH 5.5 for 20 minutes at about 0� C. Glycerol was then added to a final concentration of 15 mM to quench the reaction, and the mixture incubated a further 5 minutes at 0� C. The oxidized Mab 940 was purified by gel filtration chromatography on Sephadex G25 equilibrated with 0.1M sodium acetate buffer pH 5.5, and then reacted with 5 mM biotin-LC-hydrazide for 2 hours at room temperature with agitation. Unreacted biotin-LC-hydrazide was removed using a Sephadex G25 column equilibrated in PBS.
The density of immobilized Fab' fragments obtained using the avidin-biotin coupling chemistry was about 1.4�10-12 moles per cm2 (square centimeter).
The modified PEG was coupled to silanized-glutaraldehyde-treated waveguide surfaces prepared as described in Example I. A solution of 24 milligrams (mg) of PEG-ED powder was dissolved in 1.2 milliliters (ml) of 0.15M PBS pH 7.4 or in the same volume of 11% potassium sulfate-sodium acetate buffer at pH 5.2. The prepared waveguide surfaces were immersed in the PEG-ED solution and incubated at 60� C. for about 24 hours. The procedure using K2 SO4 -acetate buffer yielded a higher density of PEG molecules attached to the surface than that using PBS buffer. Antibodies or other binding proteins were immobilized to the PEG-coated waveguides as follows. A solution of about 3 mg/ml of antibody was dissolved in 0.15 M sodium acetate buffer, pH 5.2. A solution of equivalent weight of 50 mM sodium metaperiodate (NaIO4) was then added, and the reactants were agitated at room temperature for about an hour. Unreacted sodium metaperiodate was removed by passing the reaction mixture through a desalting column (type PD-10 from Pharmacia), which had been pre-equilibrated with the sodium acetate buffer.
Absolute antigen binding was determined by means of radiolabelled tracers or capture molecules. For example, radiolabelled BSA-FL9 was allowed to be coupled with immobilized Fab' fragments for 5 or 60 minutes in phosphate buffer pH 7.3 at room temperature. The tracer concentration was 1.5�10-7 M. Three ml per sample of fluorescein-labelled BSA (BSA-FL9) at concentrations ranging from 10-10 M to 10-7 M was injected into the flow cell. The injection was performed over a five-minute interval. The spectrum at wavelength of 488 nm was taken and the bulk BSA-FL9 was removed by flushing with PBS buffer. Three more spectra were taken, and the fluorescein peak from 513 to 517 nm was integrated. These values were set versus the log of BSA-FL9 concentration in order to obtain the binding isotherm.
TABLE I__________________________________________________________________________Summary of Solid-Phase Immunoassays using Silica Substrates                          Absolute                    Total Non-specific                                Relative                                      Immobilized                    Binding3                          Binding4                                Non-specific                                      Antibody5                                            Specific               Coupling                    (� 10-12)                          (� 10-12)                                Binding                                      (� 10-12)                                            Activity6Surface1       Antibody2               Chemistry                    (moles/cm2)                          (moles/cm2)                                (%)   (moles/cm2)                                            (%)__________________________________________________________________________Hydrophobic Silica (DDS)       Heat Treated IgG               Random                    0.65  0.04  6.46  3.00  21.67Hydrophobic Silica (DDS)       Acid Treated IgG               Random                    0.67  0.07  9.70  2.20  30.45Hydrophobic Silica (DDS)       Fab' Fragment               Random                    0.37  0.25  69.00 1.30  28.46Silica/APS/GLU       Acid Treated IgG               Random                    0.55  0.21  38.18 3.75  14.67Silica/APS/GLU/PEG       Oxidized IgG               Specific                    0.56  0.11  19.64 2.06  27.18Silica/APS/GLU/PEG       Oxidized IgG               Random                    0.41  0.10  24.39 1.44  28.47Silica/Avidin       Biotin-IgG               Specific                    0.72  0.02  2.92  0.94  76.60Silica/Avidin       Biotin-Fab               Specific                    0.84  0.02  2.62  1.10  76.36Silica/Avidin       Biotin-Fab               Specific                    0.80  0.02  1.88  "     72.73(with biotin-PEG)Silica/Hydrogel       Oxidized IgG               Specific                    0.17  0.01  6.88  7.95  2.14(preswollen)Silica/Hydrogel       Fab'    Specific                    1.51  0.03  2.03  2.76  54.71(preswollen)       Fragment__________________________________________________________________________ 1 Abbreviations: DDS  dichlorodimethylsilane; APS  aminopropylsilane GLU  glutaraldehyde; PEG  polyethylene glycol (3400 MW); BSA  bovine seru albumin; IgG  intact immunoglobulin G; Fab' antigen binding fragment with reactive thiol group; ND  not determined 2 All immunoassays were performed with an IgG, monoclonal antibody (9-40) which binds fluorescein. 3 Amount of 125 IFluorescein-BSA which bound to silica substrate. 4 Amount of 125 IBSA which bound to silica substrate. 5 Amount of 125 I9-4O immobilized on silica substrate. 6 Percent of immobilized active sites which bound antigen molecules.
TABLE II__________________________________________________________________________Summary of Solid Phase Immunoassays Using Silica Substrates Coveredwith Hydrogel with Maleimido Reactive Groups            Total hCG                  Total hCG          Relative      Relative      Immobilized            Binding in                  Binding in Absolute Non-                                     Non-specific                                           Absolute                                                   Non-specific Binding      Antibody            5 min 60 min                        Antibody                             specific Binding                                     Binding in                                           specific                                                   Binding in Constant      (�10-12            (�10-12                  (�10-12                        Activity                             in 5 min (BSA)                                     5 min (BSA)                                           in 60 min                                                   60 min (BSA)Antibody pK0      mol/cm2)            mol/cm2)                  mol/cm2)                        (%)  (�10-12 mol/cm2)                                     (%)   (�10-12                                           mol/cm2)                                                   (%)__________________________________________________________________________Fab' from 8.85 1.39 � 0.07            0.62 � 0.03                  0.81 � 0.03                        58.3 � 0.8                             <0.01   <0.97 <0.02   <2.47Anti-hCG-AFab' from 7.89 1.29 � 0.06            0.51 � 0.02                  0.67 � 0.03                        51.9 � 0.1                             0.02 � 0.01                                     3.85 � 1.81                                           0.04 � 0.01                                                   5.91 � 1.22Anti-hCG-BFab' from 8.70 0.74 � 0.04            0.18 � 0.01                  0.41 � 0.02                        55.4 � 0.3                             <0.01   <6.22 0.03 � 0.01                                                   7.21 � 2.09Anti-hCG-CFab' from 8.00 1.45 � 0.06            0.58 � 0.02                  0.75 � 0.03                        51.4 � 0.3                             <0.01   <1.20 <0.02   <3.21Anti-hCG-DFab' from --   2.50 � 0.10            0.04 � 0.01                  0.06 � 0.02                         2.4 � 0.7                             --      --    --      --Mouse IgG__________________________________________________________________________antibody anti-hCG-A was used in the experiments whose results are depicted in FIGS. 7A, 7B, 8, 9A-9F, and 10A-10D.
F(ab')2 fragments were produced by digestion with pepsin using the procedure described by Grey and Kunkel, "H Chain subgroups of myeloma proteins and normal 7S globulin," J. Exp. Med. 120:253-266, 1964. Following digestion, F(ab')2 fragments were reduced to Fab' fragments using dithiothreitol (DTI). Specifically, 33 mg of purified antibody and 1 mg pepsin (Sigma) were dissolved in 0.1 M sodium acetate buffer (pH 4.2) and the digestion was carried out at 37� C. for 16 hours. The digestion was terminated by adjusting the pH of the reaction mixture to 8.0 with 2 M tris base. The F(ab')2 fraction was separated by gel permeation chromatography (Superdex Hiload, Pharmacia) using phosphate-buffered saline (PBS), pH 7.7, as eluent. Fab' fragments were prepared by reducing the F(ab')2 fragments (1 mg/ml) with 1.75 mM DTT and 3.5 mM ethylenediamine tetraacetate (EDTA) in 0.17 M tris buffer (pH 7.4) for 45 minutes at room temperature. After reduction, excess DTT was removed by gel permeation chromatography using a Sephadex G-25 column (Pharmacia) equilibrated in 0.1 M sodium phosphate buffer (pH 6.0) containing 5 mM EDTA.
TABLE III__________________________________________________________________________Summary of Solid Phase Immunoassay Using Silica Substrates withAdsorbed Avidin and Biotinylated Fab` Fragments                            Absolute      Immobilized              Total   Specific                            Non-specific Binding                                      Relative      Antibody              hCG Binding                      Activity                            (BSA)     Non-specific BindingAntibody   (�10-12 mol/cm2)              (�10-12 mol/cm2)                      (%)   (�10-12 mol/cm2)                                      (BSA) (%)__________________________________________________________________________Fab' from Anti-hCG-A      1.19 � 0.02              1.22 � 0.01                      100 � 5                            0.05 � 0.02                                      4.20 � 0.02Fab' from Anti-hCG-B      1.40 � 0.05              1.38 � 0.07                      98 � 9                            0.05 � 0.01                                      3.57 � 0.02Fab' from Anti-hCG-C      2.24 � 0.02              1.10 � 0.03                      49 � 3                            0.05 � 0.01                                      2.32 � 0.01Fab' from Anti-hCG-D      1.59 � 0.02              1.24 � 0.01                      78 � 2                             0.05 � 0.005                                      3.14 � 0.01Fab' from Mouse IgG      1.25 � 0.02               0.03 � 0.003                       2.4 � 0.05                            0.09 � 0.03                                      7.20 � 0.03__________________________________________________________________________
From the data in Tables IV and V, it is evident that the degree of nonspecific binding was significantly lower for the PF108 and PP105 coatings than for PF68 and PF88, and with Fab' concentrations of 1.5�10-6 M. For this reason, PF108 and PP105 are presently preferred BCPs. In general, among TBCP compounds, those exhibiting better resistance to non-specific binding (and thus presently preferred) are those having PPO segments of length about 45-50 residues or more. Desirably, the level of non-specific binding should be no more than about 10%, and preferably below about 1%-2%, of the level of specific binding. Alternately, or in addition, it is desirable that the absolute amount of non-specific binding be in the range below about 5�10-14 and preferably below about 5�10-15. Neither the hydrophilic-to-lipophilic balance of the TBCP, the total MW, or the molecular weight ratio of PEO to PPO in the compound, appear to be as important as the length of the PPO segment in selecting TBCPs with good efficiency in inhibiting non-specific binding.
TABLE V______________________________________Effects of Pluronics Coating on the Non-SpecificBinding of BSA and Fluorescein-BSA to PolystyreneExperi-         Pluronics                    Fluorescein-ment            Coating  BSA       BSANo.   Pluronics Time     (mol. cm-2)                              (mol. cm-2)______________________________________1.    None      --       6.05e-12  8.37e-132.    F108       24 hours                    5.46e-14  8.13e-153.    None      --       1.96 � 0.04e-12                              1.53 � 0.06e-124.    P105       24 hours                    <1e-15    <1e-155.    F68        24 hours                    1.68 � 0.30e-13                              0.91 � 0.32e-136.    F88        24 hours                    1.03 � 0.30e-13                              0.32 � 0.17e-137.    F108       10 min  <1e-15    <1e-158.    F108       30 min  <1e-15    <1e-159.    F108       60 min  <1e-15    <1e-1510.   F108      180 min  <1e-15    <1e-1511.   F108       24 hours                    <1e-15    <1e-15______________________________________
Silica-MSil(5000) surfaces were prepared by incubating silica chips for 40 minutes at 90� C. with a 10% aqueous solution of MSil(5000). Silica-DPS-PF108 surfaces were prepared by immersing the silica chips for 60 minutes at room temperature in a solution of 10% DPS in toluene. The DPS surfaces were washed with ethanol, then with water, and then immersed in a 4% aqueous solution of PF108 for about 24 hours at room temperature.
Silica-APS-Glu-PEG(2000) surfaces were prepared by incubating silica chips with an aqueous solution of 10% APS at room temperature for 30 minutes. The APS-coated chips were then washed with ethanol and with water, and then immersed in 2.5% aq. solution of glutaraldehyde in bicarbonate buffer, pH 8.0, for 2 hours at room temperature. The chips were washed again, and then reacted with methoxy-PEG(2000) hydrazide in acetate buffer containing 11% K2 SO4, pH 5.2 at 60� C. for about 24 hours. Silica-avidin-biotin-PEG(3400) surfaces were prepared by incubating silica chips with avidin (3�10-6 M in PBS) at room temperature for 3 hours, then washed with PBS. The
avidin-coated chips were then reacted with biotin-PEG(3400) (also 3�10-6 M in PBS) for 3 hours at room temperature.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS34394 *Feb 11, 1862Himself and GeoImproved roller for clothes-wringersUS3939350 *Apr 29, 1974Feb 17, 1976Board Of Trustees Of The Leland Stanford Junior UniversityFluorescent immunoassay employing total reflection for activationUS4050895 *Sep 17, 1976Sep 27, 1977Monsanto Research CorporationOptical analytical device, waveguide and methodUS4166105 *Dec 20, 1974Aug 28, 1979Block Engineering, Inc.Dye tagged reagentUS4235869 *May 16, 1978Nov 25, 1980Syva CompanyAssay employing a labeled Fab-fragment ligand complexUS4264766 *Sep 11, 1978Apr 28, 1981Hoffmann-La Roche Inc.Immunological diagnostic reagentsUS4298685 *May 3, 1979Nov 3, 1981Burroughs Wellcome Co.Diagnostic reagentUS4429008 *Dec 10, 1981May 16, 1995Univ CaliforniaThiol reactive liposomesUS4447546 *Aug 23, 1982May 8, 1984Myron J. BlockFluorescent immunoassay employing optical fiber in capillary tubeUS4450231 *Mar 31, 1982May 22, 1984Biostar Medical Products, Inc.Immunoassay for determination of immune complexes with polymer-coated plastic baseUS4558014 *Jun 13, 1983Dec 10, 1985Myron J. BlockAssay apparatus and methodsUS4562157 *May 25, 1984Dec 31, 1985National Research Development CorporationDiagnostic device incorporating a biochemical ligandUS4596723 *May 2, 1984Jun 24, 1986Daryl Laboratories, Inc.Immunoassay substrateUS4708871 *Mar 8, 1984Nov 24, 1987Commonwealth Serum Laboratories CommissionAntigenically active amino acid sequencesUS4722906 *Sep 29, 1982Feb 2, 1988Bio-Metric Systems, Inc.Binding reagents and methodsUS4775637 *Dec 5, 1985Oct 4, 1988Purtec LimitedAn immunoassay apparatus having at least two waveguides and method for its useUS4820649 *Sep 22, 1987Apr 11, 1989Teijin LimitedMethod and kit having layered device for detecting biological component by interference colorUS4826759 *Oct 4, 1984May 2, 1989Bio-Metric Systems, Inc.Field assay for ligandsUS4852967 *Mar 25, 1986Aug 1, 1989Ciba Corning Diagnostics Corp.Evanescent wave sensorsUS4909990 *Sep 2, 1987Mar 20, 1990Myron J. BlockImmunoassay apparatusUS4978503 *Jun 12, 1985Dec 18, 1990Ares-Serono Research & Development Limited PartnershipDevices for use in chemical test proceduresUS5002582 *Dec 8, 1989Mar 26, 1991Bio-Metric Systems, Inc.Preparation of polymeric surfaces via covalently attaching polymersUS5006333 *Jul 13, 1989Apr 9, 1991Ddi Pharmaceuticals, Inc.Conjugates of superoxide dismutase coupled to high molecular weight polyalkylene glycolsUS5006716 *Feb 22, 1989Apr 9, 1991Research Corporation Technologies, Inc.Method and system for directional, enhanced fluorescence from molecular layersUS5043278 *Jul 6, 1990Aug 27, 1991Toray Industries, Inc.Physiologically-active substance fixed to a thin fiber carrier with an alkylene oxide chainUS5061857 *Nov 9, 1990Oct 29, 1991The United States Of America As Represented By The Secretary Of The NavyWaveguide-binding sensor for use with assaysUS5071216 *Jun 28, 1990Dec 10, 1991Honeywell Inc.Optical interconnect waveguide-beam couplerUS5071217 *Apr 25, 1990Dec 10, 1991Birch Thomas FPrecision focusing and locating collar for a fiber optic connectorUS5073484 *Feb 23, 1983Dec 17, 1991Bio-Metric Systems, Inc.Quantitative analysis apparatus and methodUS5081012 *Jan 22, 1990Jan 14, 1992Applied Research Systems Ars Holding N.V.Waveguide sensor with input and reflecting gratings and its use in immunoassayUS5096807 *Dec 1, 1989Mar 17, 1992Murex CorporationImaging immunoassay detection system with background compensation and its useUS5135876 *Sep 24, 1987Aug 4, 1992University Of UtahMethod and apparatus for the regulation of complex bindingUS5156976 *Jun 7, 1991Oct 20, 1992Ciba Corning Diagnostics Corp.Evanescent wave sensor shell and apparatusUS5166515 *Jun 21, 1990Nov 24, 1992Applied Research Systems Ars Holding, N.V.Method of optical analysis of a fluid including an optical waveguideUS5168537 *Jun 28, 1991Dec 1, 1992Digital Equipment CorporationMethod and apparatus for coupling light between an optoelectronic device and a waveguideUS5182216 *Jan 20, 1988Jan 26, 1993Unilever Patent Holdings B.V.Assays and devices thereforUS5242828 *Nov 9, 1989Sep 7, 1993Pharmacia Biosensor AbSensing surfaces capable of selective biomolecular interactions, to be used in biosensor systemsUS5248620 *Jun 25, 1991Sep 28, 1993Boehringer Mannheim GmbhNon-ionic block copolymers of propylene oxide and ethylene oxideUS5344784 *Nov 28, 1989Sep 6, 1994Applied Research Systems Ars Holding N.V.Fluorescent assay and sensor thereforUS5436161 *Jul 22, 1994Jul 25, 1995Pharmacia Biosensor AbMatrix coating for sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systemsUS5552272 *Jun 10, 1993Sep 3, 1996Biostar, Inc.Detection of an analyte by fluorescence using a thin film optical deviceEP0478137A2 *Aug 19, 1991Apr 1, 1992Gec-Marconi LimitedAn optical sensorGB2220083A * Title not availableWO1981000912A1 *Sep 19, 1980Apr 2, 1981R BucklesMethod and apparatus for analysisWO1989009408A1 *Mar 28, 1989Oct 5, 1989Ares-Serono Research & Development Limited PartnerMethod of assayWO1990001166A1 *Jul 25, 1989Feb 8, 1990Ares-Serono Research & Development Limited PartnershipMethod of assayWO1990006503A2 *Nov 28, 1989Jun 14, 1990Applied Research Systems Ars Holding N.V.Sensor for optical assayWO1991013339A1 *Mar 4, 1991Sep 5, 1991Fisons PlcSample cell for use in chemical or biochemical assaysWO1991015751A1 *Apr 10, 1991Oct 17, 1991Applied Research Systems Ars Holding N.V.Method of improving assay sensitivityWO1992009892A1 *Nov 21, 1991Jun 11, 1992Applied Research Systems Ars Holding N.V.Method of assayWO1992016838A1 *Mar 20, 1992Oct 1, 1992Gec-Marconi LimitedSeparation method* Cited by examinerNon-Patent CitationsReference1Caldwell, Li, Li and Dalgleish, "Adsorption behavior of milk proteins of polystrene ltex", Elsevier Science Publishers, J. Chromatography 604: 63-71, 1992.2 *Caldwell, Li, Li and Dalgleish, Adsorption behavior of milk proteins of polystrene ltex , Elsevier Science Publishers, J. Chromatography 604: 63 71, 1992.3Carlsson, "Protein Thiolation and Reversible Protein-Protein Conjugation", Biochem J., 173, pp. 723-737, 1978.4 *Carlsson, Protein Thiolation and Reversible Protein Protein Conjugation , Biochem J., 173, pp. 723 737, 1978.5Ciana, Leopoldo Della, "Electrochemiluminescence Immunosensors And DNA Probes For Clinical Diagnostics", Abstract, p. 19.6 *Ciana, Leopoldo Della, Electrochemiluminescence Immunosensors And DNA Probes For Clinical Diagnostics , Abstract, p. 19.7Harrick, N.J., and Loeb, George I., "Multiple Internal Reflection Fluorscence Spectrometry", Analytical Chemistry, vol. 45, No. 4, Apr. 1973, pp. 687-691.8 *Harrick, N.J., and Loeb, George I., Multiple Internal Reflection Fluorscence Spectrometry , Analytical Chemistry, vol. 45, No. 4, Apr. 1973, pp. 687 691.9Herron, "Fluorescent immunosensors using planar waveguides", SPIE, vol. 1885, Proceedings of Advances in Fluorescence Sensing Technology, pp. 28-39, 1993.10 *Herron, Fluorescent immunosensors using planar waveguides , SPIE, vol. 1885, Proceedings of Advances in Fluorescence Sensing Technology, pp. 28 39, 1993.11 *Huang, Shao Chie, PEG Derivatives As Tethers For Site Directed Immobilization Of Oxidized Antibody , Master s Thesis, Nov. 1991.12 *Huang, Shao Chie, PEG derivatives as tethers for site directed immobilization of oxidized antibody , title page, abstract (pp. iv v), and pp. 35 39.13Huang, Shao-Chie, "PEG Derivatives As Tethers For Site-Directed Immobilization Of Oxidized Antibody", Master's Thesis, Nov. 1991.14Huang, Shao-Chie, "PEG derivatives as tethers for site-directed immobilization of oxidized antibody", title page, abstract (pp. iv-v), and pp. 35-39.15Ives, Jeffrey T., "Optical waveguide sensors: characterization of evanescent and scatter excitation," Ph.D. dissertation submitted to Dept. of Bioengineering, University of Utah, Jun. 1990.16 *Ives, Jeffrey T., Optical waveguide sensors: characterization of evanescent and scatter excitation, Ph.D. dissertation submitted to Dept. of Bioengineering, University of Utah, Jun. 1990.17Lakowicz, Joseph R., "Fluorescence Lifetime-Based Sensing And Imaging", Abstract, p. 5.18 *Lakowicz, Joseph R., Fluorescence Lifetime Based Sensing And Imaging , Abstract, p. 5.19Lee, "Protein-resistant surfaces prepared by PEO-containing block copolymer surfactants", Journal of Biomedical Materials Research, pp. 351-368, 1989.20 *Lee, Protein resistant surfaces prepared by PEO containing block copolymer surfactants , Journal of Biomedical Materials Research, pp. 351 368, 1989.21Leiner, M.J.P., "Optical Sensors For In Vitro Blood Gas Analysis", Abstract.22 *Leiner, M.J.P., Optical Sensors For In Vitro Blood Gas Analysis , Abstract.23Ligler et al., "Evanescent Wave Optical Fibre Sensors", Abstract, p. 75.24 *Ligler et al., Evanescent Wave Optical Fibre Sensors , Abstract, p. 75.25Liu, Benjamin L., "Clinical Requirements For Optical Immunosensors", Abstract, p. 13.26 *Liu, Benjamin L., Clinical Requirements For Optical Immunosensors , Abstract, p. 13.27Masuda, Kenji, "Study Of The Sensitivity Of Evanescent Wave Immunoassay", Abstract, p. 11.28 *Masuda, Kenji, Study Of The Sensitivity Of Evanescent Wave Immunoassay , Abstract, p. 11.29 *O Keeffe et al., Development Of A LED Based Phase Fluorimetric Oxygen Sensor Using Evanescent Wave Excitation Of A Sol Gel Immobilized Dye , Abstract, p. 149.30 *O Neill et al., Use Of An Optical Biosensor To Measure Prostate Specific Antigen In Whole Blood , Abstract, p. 20.31O'Keeffe et al., "Development Of A LED Based Phase Fluorimetric Oxygen Sensor Using Evanescent Wave Excitation Of A Sol-Gel Immobilized Dye", Abstract, p. 149.32O'Neill et al., "Use Of An Optical Biosensor To Measure Prostate Specific Antigen In Whole Blood", Abstract, p. 20.33Particle Size Distribution II, Li, J., Caldwell, K.D., and Tan, J.S., "Size analysis of a block copolymercoated polystyrene latex", Chapter 16, pub. American Chemical Society, 1991.34 *Particle Size Distribution II, Li, J., Caldwell, K.D., and Tan, J.S., Size analysis of a block copolymercoated polystyrene latex , Chapter 16, pub. American Chemical Society, 1991.35Robinson, Grenville, "The Commercial Development Of Planar Optical Biosensors", Abstract.36 *Robinson, Grenville, The Commercial Development Of Planar Optical Biosensors , Abstract.37Sacco et al., "Fibre-Fluorescence Immunosensors Based On Evanescent Wave Detection", Abstract, p. 52.38 *Sacco et al., Fibre Fluorescence Immunosensors Based On Evanescent Wave Detection , Abstract, p. 52.39Slovacek et al., "Application Of A Plastic Evanescent Wave Sensor To Immunological Measurements Of CKMB", Abstract, p. 16.40 *Slovacek et al., Application Of A Plastic Evanescent Wave Sensor To Immunological Measurements Of CKMB , Abstract, p. 16.41van Erp et al., "Affinity of monoclonal antibodies", J. Immunol. Methods, 140:235-241, 1991.42 *van Erp et al., Affinity of monoclonal antibodies , J. Immunol. Methods, 140:235 241, 1991.* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS6096172 *Oct 19, 1998Aug 1, 2000Motorola, Inc.Method of bonding bio-molecules to a test siteUS6232124Nov 13, 1998May 15, 2001Verification Technologies, Inc.Automated fingerprint methods and chemistry for product authentication and monitoringUS6242267 *Mar 19, 1997Jun 5, 2001University Of Utah Research FoundationOscillation apparatus and methods for multi-analyte homogeneous fluoro-immunoassaysUS6274872 *Jul 11, 1997Aug 14, 2001Icb Institut Fuer Chemo- Und Biosensorik Menster E.V.Process and device for carrying out quantitative, fluorescence affinity testsUS6285807 *Nov 16, 1998Sep 4, 2001Trustees Of Tufts CollegeFiber optic sensor for long-term analyte measurements in fluidsUS6316274Mar 1, 2000Nov 13, 2001University Of Utah Research FoundationApparatus and methods for multi-analyte homogeneous fluoro-immunoassaysUS6338968 *Aug 2, 1999Jan 15, 2002Signature Bioscience, Inc.Method and apparatus for detecting molecular binding eventsUS6346376 *May 11, 1999Feb 12, 2002Centre Suisse D'electronique Et De Mictotechnique SaOptical sensor unit and procedure for the ultrasensitive detection of chemical or biochemical analytesUS6368795 *Feb 1, 1999Apr 9, 2002Signature Bioscience, Inc.Bio-assay device and test system for detecting molecular binding eventsUS6376258 *Jan 10, 2000Apr 23, 2002Signature Bioscience, Inc.Resonant bio-assay device and test system for detecting molecular binding eventsUS6395480Feb 1, 1999May 28, 2002Signature Bioscience, Inc.Computer program and database structure for detecting molecular binding eventsUS6448089Oct 12, 1999Sep 10, 2002Aurora Biosciences CorporationMultiwell scanner and scanning methodUS6458595Jan 24, 2001Oct 1, 2002Verification Technologies, Inc.Automated fingerprint methods and chemistry for product authentication and monitoringUS6465054 *Oct 20, 1998Oct 15, 2002Roche Diagnostics GmbhProcess for coating surfacesUS6485905 *Aug 2, 1999Nov 26, 2002Signature Bioscience, Inc.Bio-assay deviceUS6490030Jan 18, 1999Dec 3, 2002Verification Technologies, Inc.Portable product authentication deviceUS6512580Oct 27, 1999Jan 28, 2003Verification Technologies, Inc.Method and apparatus for portable product authenticationUS6586257Sep 19, 2000Jul 1, 2003Vertex Pharmaceuticals IncorporatedMultiwell scanner and scanning methodUS6589626Mar 29, 2001Jul 8, 2003Verification Technologies, Inc.Copy-protected optical media and method of manufacture thereofUS6597499Jan 25, 2001Jul 22, 2003Olympus Optical Co., Ltd.Total internal reflection fluorescence microscope having a conventional white-light sourceUS6638483Jul 31, 2002Oct 28, 2003Vertex Pharmaceuticals IncorporatedMultiwell scanner and scanning methodUS6638593Jun 12, 2001Oct 28, 2003Verification Technologies, Inc.Copy-protected optical media and method of manufacture thereofUS6707539Oct 18, 2002Mar 16, 2004Verification Technologies, Inc.Portable product authentication deviceUS6814933May 8, 2003Nov 9, 2004Aurora Biosciences CorporationMultiwell scanner and scanning methodUS6887427Jun 17, 2002May 3, 2005Roche Diagnostics GmbhCoated biosensorUS6888636 *Mar 13, 2002May 3, 2005E. I. Du Pont De Nemours And CompanyMethod and apparatus for measuring the color properties of fluidsUS6979567Nov 13, 2001Dec 27, 2005Biocentrex, LlcApparatus and methods for multi-analyte homogeneous fluoro-immunoassaysUS7101945Feb 20, 2002Sep 5, 2006Bayer AktiengesellschaftPhosphorus-containing polymers for optical signal transducersUS7122790 *May 13, 2002Oct 17, 2006The Penn State Research FoundationMatrix-free desorption ionization mass spectrometry using tailored morphology layer devicesUS7175811Apr 30, 2001Feb 13, 2007Edgelight BiosciencesMicro-array evanescent wave fluorescence detection deviceUS7332327Jun 10, 2002Feb 19, 2008Bionavis Ltd.Method and biosensor for analysisUS7391018Sep 14, 2005Jun 24, 2008Nanosys, Inc.Nanostructured thin films and their usesUS7517705Aug 21, 2006Apr 14, 2009Bayer AktiengesellschaftPhosphorus-containing polymers for optical signal transducersUS7544504 *Jun 10, 2002Jun 9, 2009Bionavis Ltd.Diagnostic methodsUS7616844 *Mar 14, 2007Nov 10, 2009Invivosense AsFabrication of fiber optic probesUS7660415Feb 9, 2010Selinfreund Richard HMethod and apparatus for controlling access to storage mediaUS7755038May 15, 2008Jul 13, 2010Nanosys, Inc.Nanostructured thin films and their usesUS7842498Nov 5, 2002Nov 30, 2010Bio-Rad Laboratories, Inc.Hydrophobic surface chipUS7911615 *Mar 22, 2011E. I. Du Pont De Nemours And CompanyMethod and apparatus for measuring the color properties of fluidsUS7951583May 31, 2011Plc Diagnostics, Inc.Optical scanning systemUS8187866May 17, 2011May 29, 2012Plc Diagnostics, Inc.Optical scanning systemUS8288157Oct 16, 2012Plc Diagnostics, Inc.Waveguide-based optical scanning systemsUS8300993 *Nov 12, 2009Oct 30, 2012Mbio Diagnostics, Inc.Waveguide with integrated lensUS8331751 *Nov 9, 2010Dec 11, 2012mBio Diagnositcs, Inc.Planar optical waveguide with core of low-index-of-refraction interrogation mediumUS8465699Jun 18, 2013Pacific Biosciences Of California, Inc.Illumination of integrated analytical systemsUS8467061Jun 18, 2013Pacific Biosciences Of California, Inc.Integrated analytical system and methodUS8501122Dec 8, 2010Aug 6, 2013Affymetrix, Inc.Manufacturing and processing polymer arraysUS8586347Sep 15, 2011Nov 19, 2013Mbio Diagnostics, Inc.System and method for detecting multiple molecules in one assayUS8606066Dec 4, 2012Dec 10, 2013Mbio Diagnostics, Inc.Planar optical waveguide with core of low-index-of-refraction interrogation mediumUS8649011May 16, 2013Feb 11, 2014Pacific Biosciences Of California, Inc.Integrated analytical system and methodUS8675199Apr 28, 2010Mar 18, 2014Plc Diagnostics, Inc.Waveguide-based detection system with scanning light sourceUS8747751Jun 10, 2009Jun 10, 2014Plc Diagnostics, Inc.System and method for nucleic acids sequencing by phased synthesisUS8795727Nov 5, 2010Aug 5, 2014Spotlight Technology Partners LlcFragmented hydrogelsUS8841138Jul 28, 2004Sep 23, 2014Jsr CorporationSurface of base material being inhibited in non-specific adsorptionUS8867038Dec 16, 2013Oct 21, 2014Pacific Biosciences Of California, Inc.Integrated analytical system and methodUS8994946Dec 21, 2012Mar 31, 2015Pacific Biosciences Of California, Inc.Integrated analytical system and methodUS9157864May 16, 2013Oct 13, 2015Pacific Biosciences Of California, Inc.Illumination of integrated analytical systemsUS9212995Mar 15, 2013Dec 15, 2015Mbio Diagnostics, Inc.System and method for detecting multiple molecules in one assayUS9223084Dec 16, 2013Dec 29, 2015Pacific Biosciences Of California, Inc.Illumination of optical analytical devicesUS9289449Jul 23, 2014Mar 22, 2016Spotlight Technology Partners LlcHydrogel compositionsUS9291568Sep 4, 2014Mar 22, 2016Pacific Biosciences Of California, Inc.Integrated analytical system and methodUS9291569Mar 9, 2015Mar 22, 2016Pacific Biosciences Of California, Inc.Optics collection and detection system and methodUS9372308Jun 17, 2013Jun 21, 2016Pacific Biosciences Of California, Inc.Arrays of integrated analytical devices and methods for productionUS20020114604 *Feb 20, 2002Aug 22, 2002Ingmar DornPhosphorus-containing polymers for optical signal transducersUS20020149773 *Mar 13, 2002Oct 17, 2002Martino Anthony JosephMethod and apparatus for measuring the color properties of fluidsUS20020187312 *May 13, 2002Dec 12, 2002The Penn State Research FoundationMatrix-free desorption ionization mass spectrometry using tailored morphology layer devicesUS20030032067 *Sep 10, 2002Feb 13, 2003Signature Bioscience Inc.Test systems and sensors for detecting molecular binding eventsUS20030059954 *Jun 10, 2002Mar 27, 2003Inger VikholmMethod and biosensor for analysisUS20030124371 *Nov 5, 2002Jul 3, 2003Ciphergen Biosystems, Inc.Hydrophobic surface chipUS20030124632 *Jun 10, 2002Jul 3, 2003Vesa KleimolaDiagnostic methodsUS20030132406 *Feb 16, 2001Jul 17, 2003Ralf WaldhauslSensor element for optically detecting chemical or biochemical analytesUS20030203490 *May 8, 2003Oct 30, 2003Vuong T. MinhMultiwell scanner and scanning methodUS20040082058 *Oct 29, 2002Apr 29, 2004Arthur SchleiferArray hybridization apparatus and method for making uniform sample volumesUS20040214310 *Apr 25, 2003Oct 28, 2004Parker Russell A.Apparatus and method for array alignmentUS20050009026 *Aug 24, 2002Jan 13, 2005Abel Andreas PeterSurface for the immobilisation of nucleic acidsUS20050106615 *Nov 17, 2004May 19, 2005Affymetrix, Inc.Bioarray chip reaction apparatus and its manufactureUS20050106617 *Nov 22, 2004May 19, 2005Affymetrix, Inc., A Delaware CorporationBioarray chip reaction apparatus and its manufactureUS20050112757 *Dec 17, 2004May 26, 2005Affymetrix, Inc.Apparatus and methods for constructing array platesUS20050112773 *Nov 5, 2004May 26, 2005Aurora Biosciences CorporationMultiwell scanner and scanning methodUS20050124008 *Dec 3, 2004Jun 9, 2005Kauvar Lawrence M.Homogeneous competition assaysUS20050153320 *Nov 8, 2004Jul 14, 2005Herron James N.Single base extensionUS20050163663 *Mar 22, 2005Jul 28, 2005Martino Anthony J.Method and apparatus for measuring the color properties of fluidsUS20050196761 *Mar 8, 2004Sep 8, 2005Thompson Allen C.Array hybridization apparatus and methodUS20050202445 *Mar 9, 2004Sep 15, 2005Thompson Allen C.Thermoplastic array hybridization apparatus and methodUS20050213914 *Mar 23, 2004Sep 29, 2005Motorola, Inc.High efficiency light guideUS20060240438 *Jul 28, 2004Oct 26, 2006Yukio NagasakiSurface of base material being inhibited in non-specific adsorptionUS20060287453 *Aug 21, 2006Dec 21, 2006Bayer AktiengesellschaftPhosphorus-containing polymers for optical signal transducersUS20070003201 *Aug 31, 2006Jan 4, 2007Motorola, Inc.High efficiency light guideUS20070196863 *Feb 16, 2007Aug 23, 2007Hanson Technologies, Inc.Prion protein detectionUS20070211985 *Mar 8, 2007Sep 13, 2007Plc Diagnostics, Inc.Optical Scanning SystemUS20080073505 *Sep 14, 2005Mar 27, 2008Nanosys, Inc.Nanostructured thin films and their usesUS20090068668 *Sep 12, 2008Mar 12, 2009Plc Diagnostics, Inc.Waveguide-Based Optical Scanning SystemsUS20090074349 *Mar 14, 2007Mar 19, 2009Invivosense AsFabrication of fiber optic probesUS20090124034 *May 15, 2008May 14, 2009Nanosys, Inc.Nanostructured Thin Films and Their UsesUS20090270269 *Apr 28, 2008Oct 29, 2009Ashok KumarNano-scale fluoro-biosensors exhibiting a low false alarm rate for rapid detection of biological contaminantsUS20100220318 *Nov 12, 2009Sep 2, 2010Moll Kevin DWaveguide With Integrated LensUS20110014436 *Jan 20, 2011Nanoink, Inc.Methods for forming hydrogels on surfaces and articles formed therebyUS20110049388 *Nov 9, 2010Mar 3, 2011Mbio Diagnostics, Inc.Planar optical waveguide with core of low-index-of-refraction interrogation mediumUS20150093816 *Dec 11, 2014Apr 2, 2015Imec VzwCompact Fluid Analysis Device and Method to FabricateUSH2223Apr 4, 2003Sep 2, 2008The United States Of America As Represented By The Secretary Of The NavyPatterned, micrometer-sized antibody featuresDE102009035502A1 *Jul 30, 2009Feb 3, 2011Universit�tsklinikum JenaVerfahren und Vorrichtung zur Erfassung der Bewegung und Anlagerung von Zellen und Partikeln an Zell-, Gewebe- und Implantatschichten bei der Simulation von FlussbedingungenEP1650565A1 *Jul 28, 2004Apr 26, 2006Tokyo University of Science, Educational FoundationSurface of base material being inhibited in non-specific adsorptionEP1912740A1 *Jun 8, 2006Apr 23, 2008Amic ABOptical assay systemEP1912740A4 *Jun 8, 2006Jan 11, 2012Amic AbOptical assay systemWO2003020966A2 *Aug 24, 2002Mar 13, 2003Zeptosens AgSurface for the immobilisation of nucleic acidsWO2003020966A3 *Aug 24, 2002Nov 20, 2003Zeptosens AgSurface for the immobilisation of nucleic acidsWO2003040700A1 *Nov 7, 2002May 15, 2003Ciphergen Biosystems, Inc.Hydrophobic surface chipWO2003056338A1 *Sep 24, 2002Jul 10, 2003Biofons LtdDiagnostic methodsWO2005096046A1 *Mar 22, 2005Oct 13, 2005Motorola, Inc.High efficiency light guideWO2014040730A1 *Sep 11, 2013Mar 20, 2014Fraunhofer-Gesellschaft zur F�rderung der angewandten Forschung e.V.Measuring device for luminescence measurement* Cited by examinerClassifications U.S. Classification436/518, 427/337, 427/163.2, 427/2.11, 436/527, 435/288.7, 385/129, 436/165, 436/531, 435/287.1, 427/2.13, 436/805, 435/287.2, 436/164, 436/807, 427/338, 385/12, 427/414, 385/130, 436/512, 436/172, 435/808, 427/162, 435/7.5International ClassificationC07C281/02, G01N21/77, G01N21/25, G01N33/543, G01N21/64, C08G65/329Cooperative ClassificationY10S436/807, Y10S436/805, Y10S435/808, G01N33/54393, G01N21/648, G01N21/6428, C07C281/02, G01N33/54353, G01N33/54373, G01N21/6452, C08G65/329, G01N21/7703European ClassificationG01N21/77B, G01N33/543F, C07C281/02, G01N33/543K2, G01N21/64H, C08G65/329, G01N21/64P8, G01N21/64P2, G01N33/543MLegal EventsDateCodeEventDescriptionJun 20, 2000CCCertificate of correctionJan 22, 2003REMIMaintenance fee reminder mailedMar 25, 2003SULPSurcharge for late paymentMar 25, 2003FPAYFee paymentYear of fee payment: 4Oct 2, 2006FPAYFee paymentYear of fee payment: 8Dec 27, 2010FPAYFee paymentYear of fee payment: 12RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services

value: This website is a search engine provided by Google. Handling over 3.5 billion searches per day, it has a 92% share of the global search engine market.


text:
Amazon.co.uk: Caz's review of
Sleeping aid, 13 Jun. 2013
I bought these after they were recommended to me by a family member. If & when I actually do fall asleep, they do seem to give me a reasonably nice refreshing kip.
B0045XGE9E Welcome Sleeping aid I bought these after they were recommended to me by a family member. If & when I actually do fall asleep, they do seem to give me a reasonably nice refreshing kip. Caz 13 Jun. 2013
Top Reviewer Ranking: 324,907

type: global

value: This website is an American multinational technology company which focuses on e-commerce, cloud computing, digital streaming, and artificial intelligence.


text:
Paper Dolls of the 1930's 1976 Printing Janet Nason and
Dolls of the 1930's, Paper Dolls Including Chuckles, May-Belle, Dottie, Gretchen, Treasure Chest, Paper Clothes with tabs, Paper Furniture, Paper Baby Carriage, and several toys and accessories.
Booklet size: 14-1/4 inches x 11 inches.
Condition: Vintage New Old Stock. Very Good condition, unused, uncut.
Vintage 1967 Dinner Menu from Incres Line Luxury Cruise Ship m/s Victoria Cover has Lithograph of Fort Louis on the Island of Martinique
Vintage 1967 m/s Victoria Cruise Ship Luncheon Menu and Captains Dinner Menu w/ Humorous Neoclassical Print Titled "Disappointed Epicures"
Antique Childs Cabinet Photo Early 1900s Photograph of Young Girl in Victorian or Edwardian Era Attire
Antique Vintage Large Paper Dolls 30's/40's....at least 50 pc ....These ARE NOT reproductions...dolls/clothes/accesories
Vintage 1979 1980 Whitman Ballerina Man John, Ballerina Judy, Strawberry Sue Cut-Out Paper Dolls outfits Collectible paper ephemera
Dolls Of The 1930s Paper Dolls Janet Nason 1976
Vintage 1960s Paper Dolls Japan
Vintage Paper Doll Set with Mother, Child, and Clothing (1940s) - Ephemera, Collectible, Paper Projects
Vintage Antique German Bisque Paper Doll book by Janet Nason
Doris Day(An M-G-M Star) Paper Dolls-Whitman Publishing-1955-2 Statuette Dolls and Clothes(Uncut) in Folder-Vintage Paperdolls
Vintage Triplet Dolls paper dolls Stephens Publishing #176 uncut
Original 1936 Polly Pepper Paper Dolls
Antique Paper Dolls 1915-1920, Dover Reprint, 1975. UNCUT Paper Dolls.
Antique Paper Dolls-The Edwardian Era 1975, Paper Dolls, Vintage Paper Dolls
PaperDolls "BarbaraStanwyck" PaperDollCutouts, Girls'Dolls,VintagePaperDoll, WomenOfTheTheSilverScreen, NostalgiaToys
vintage Disney 7 Dwarfs paper dolls and Bambi pack

value: This website is an American e-commerce company focused on handmade or vintage items and craft supplies.


text:
He came from an Oklahoma farm in April, 1938. Became a migratory farm worker in California, joined the United Agricultural Packing and Allied Workers of America (Congress of Industrial Organization-CIO) at the beginning of the cotton strike of October, 1938 and became the leader of the "Flying Squadron" which attempted to picket the large fields of corporation farms by automobile caravans. He drove the first car. Kern County, California skip navigation
link: "//www.loc.gov/pictures/item/fsa2000002149/PP/",
url :"//cdn.loc.gov/service/pnp/fsa/8b32000/8b32700/8b32703_150px.jpg",
link :"//cdn.loc.gov/service/pnp/fsa/8b32000/8b32700/8b32703_150px.jpg",
link :"//cdn.loc.gov/service/pnp/fsa/8b32000/8b32700/8b32703r.jpg",
link :"//cdn.loc.gov/service/pnp/fsa/8b32000/8b32700/8b32703v.jpg",
link :"//cdn.loc.gov/master/pnp/fsa/8b32000/8b32700/8b32703u.tif",
link :"//cdn.loc.gov/master/pnp/fsa/8b32000/8b32700/8b32703a.tif",
http://hdl.loc.gov/loc.pnp/fsa.8b32703
Title: He came from an Oklahoma farm in April, 1938. Became a migratory farm worker in California, joined the United Agricultural Packing and Allied Workers of America (Congress of Industrial Organization-CIO) at the beginning of the cotton strike of October, 1938 and became the leader of the "Flying Squadron" which attempted to picket the large fields of corporation farms by automobile caravans. He drove the first car. Kern County, California
Reproduction Number: LC-DIG-fsa-8b32703 (digital file from original neg.)
LC-USF34-018616-C (b&w film nitrate neg.)
Call Number: LC-USF34- 018616-C [P&P] LOT 0346 (corresponding photographic print)
Film copy on SIS roll 27, frame 1935.
http://www.loc.gov/pictures/item/fsa2000002149/PP/
Price lists, contact information, and order forms are available on theDuplication Services Web site. Call Number: LC-USF34- 018616-C [P&P] LOT 0346 (corresponding photographic print)

value: This website is the research library that officially serves the United States Congress and is the de facto national library of the United States. It is the oldest federal cultural institution in the United States.


text:
Erin S. - Eastside Gals Swap Meetup Group (Utica, MI)
https://secure.meetupstatic.com/photos/member/1/3/b/2/member_132665042.jpeg
https://www.meetup.com/swappers/members/102416362/
Erin here; I'm married no kids. I don't work due to health issues, but I don't let it keep me down. I'm into so much I wouldn't know where to begin. I like to continue educating myself, all forms of arts, reading, traveling, & so on. I live life.
2,966 Natural Health Seekers
2,302 Do-Gooders

value: This website is a service used to organize online groups that host in-person and virtual events for people with similar interests. It was founded in 2002 by Chairman Scott Heiferman and four co-founders.


text:
128,737 helm ftp server jobs found, pricing in USD
500 Server Error 2 days left
i need server to my app chat 2 days left
Custom HTML Coding & CSS for Website Project (Project: BC-AAME1) 2 days left
Website that shows information from a .CSV file, and also collectes all in a database. 2 days left
Set up Softether VPN Server 2 days left
Optimization of Linux CENTOS 7/ PLESK server with high traffic 2 days left
httpd issue in centos server 1 day left
Create a deployment for my /25 IP Proxy Server 1 day left
Edit fivem server and create some script | Editar servidor de fivem y crear algun script 1 day left
Need help with moving a Ruby on Rails Website to a new server 1 day left
C# SQL Server Messing #1 1 day left
Modify OraSchool v5.0, online school system (script) based on based on PHP, MySql (Software Framework Laravel) 1 day left
...Students username : student password : student123 Parents username : parent password : parent123 we have purchased and hosted the script on our testing server with FTP access to it, we will provide access once we select a freelancer kindly submit ur bids including price, starting and delivery date this script is based on based on PHP
connect google sheet to JIRA server 1 day left
Someone to manage mp4 movie files in a server 1 day left
small php tasks, work must be done on anydesk 1 day left
Server Setup of Linux ( CentOS) 1 day left
WireGuard VPN Server 1 day left
Expert for Re-deploy Server, Net. Setup, Monitor. 1 day left
Hossting server setup - plesk for windows/centos 1 day left
Develop Automated Shopify Dropshipping Store Ready To Sales with Bigbuy. 21 hours left
Install a Gluu Server Cluster for my IDP 21 hours left
Create me a robots.txt file to delay crawlers from pinterest and optimise my server 20 hours left
PLEASE READ BEFORE BIDDING [SERVER ADMIN {RANCHER} ] 20 hours left
Fix SLL certificate issue for all my websites install on godaddy server. -- 19 hours left
Windows Virtual Server Maintenance 19 hours left
smtp server that sends unlimited email and must be inbox to hotmail gmail yahoo 18 hours left
SQL Server Scripting with SQLCMD 18 hours left
Install OpenVPN Server and settings 18 hours left
Bulk Email Server Setup 16 hours left
Need and ios developper with experience in EJABBERD XMPP server 14 hours left
Create sock5 proxy server 14 hours left
Showing 101 to 150 of 128,737 entries

value: This website is an Australian freelance marketplace website, which allows potential employers to post jobs that freelancers can then bid to complete. Founded in 2009, its headquarters is located in Sydney, Australia, though it also has offices in Vancouver, London, Buenos Aires, Manila, and Jakarta.


text:
Latest News for: golf australia
Florida Atlantic at North Texas 6.30 p.m.�CBSSN Golf ... RSM Classic 10.30 a.m.�GOLF ... CME Group Championship 1.30 p.m.�GOLF. PGA Australia. Emirates Australian Open 5 p.m.�GOLF ... DP World Championship midnight�GOLF NBA ... CBSSN Golf ... RSM Classic 10.30 a.m.�GOLF ... CME Group Championship 1.30 p.m.�GOLF. PGA Australia. Emirates Australian Open 5 p.m.�GOLF....
SYDNEY (AP) — Byeong Hun An overcame four-seasons-in-one-day type weather to take a one-stroke lead after the first round of the Australian Open ... An says. "It was weird ... ———. More AP golf. https.//apnews.com/apf-Golf and https.//twitter.com/AP—Sports ... Sports Golf Men's Golf Men's Sports ... Sydney New South Wales Australia Oceania ... ....
Vasiljkovic was extradited to Croatia in 2015 from Australia, where he had been working as a golf instructor under the name Daniel Snedden. He had moved to Australia at the age of 14 but returned to Yugoslavia before the start of the 1990s conflicts and was then sent by the ......
Golf Australia boss Stephen Pitt has been predicting a "handing of the baton" as a wave of exciting youngsters led by defending champion Cameron Davis, Brett Coletta, Anthony Quayle, pro debutant Zach Murray and Jake McLeod look to emerge from the shadows of Day, Scott and Marc Leishman....
Kuchar is staying in Australia next week for the World Cup of Golf ... When the Presidents Cup last was in Australia in 2011, 13 players from the U.S ... "This is great news for the Australian Open and we're excited about what this means for Australian golf fans," Golf Australia chief executive Stephen Pitt said....
It has already been a groundbreaking week; Postiga found himself on the back page of one of Australia’s biggest-selling newspapers and Golf Australia’s initiative in holding the AAAC alongside the Open is a world-first ... Rolls said playing golf had played a significant part in his recovery, notably with his mindset....
BC-GLF--Golf Notes,1226 ... AP Golf Writer ... Kuchar is staying in Australia next week for the World Cup of Golf ... When the Presidents Cup last was in Australia in 2011, 13 players from the U.S ... "This is great news for the Australian Open and we're excited about what this means for Australian golf fans," Golf Australia chief executive Stephen Pitt said....
Sports on TV/radio. TODAY. College basketball. Michigan at Villanova. 6.30 p.m. FS1. E. Michigan at Duke. 7 p.m. Fox Sun. LaSalle at Florida. 7 p.m. 1250-AM. Seton Hall at Nebraska ... FS1 ... Golf. PGA of Australia ... Golf ... ....
But after hours of winner's obligations, not even a private jet to Los Angeles was enough for Kuchar to make his connecting flight to Australia and the 40-year-old was stuck in LA for 24 hours with his wife and children ... It has led to a heavy criticism against Open organisers Golf Australia that the event lacks star power ... ....
Golf Australia concedes it could have handled the Adam Scott affair better, but remains confident the former world No.1 will be back to challenge for the Stonehaven Cup ... If Scott harbours any lingering ill-feelings, Pitt said Golf Australia would do everything it could to repair the relationship....

value: This website is a news aggregator founded in March 1995 and launched online in 1998. In 2003, Search Engine Watch praised the service for its "Special Reports", and called it "an interesting alternative" to other news aggregation services.


text:
Superior Collection Luxurious Egyptian Cotton Unisex Terry Bath Robe | Overstock.com Shopping - The Best Deals on Bath Robes
http://www.overstock.com/Bedding-Bath/Superior-Collection-Luxurious-Egyptian-Cotton-Unisex-Terry-Bath-Robe/6632152/product.html?recommendation_id=f39738ed-6ede-4cc1-b578-2ce2790bd6de&rcmndsrc=6&atcError=3

value: This website is an American internet retailer selling primarily furniture headquartered in Midvale, Utah, near Salt Lake City.


text:
100LED Warm White Fairy Christmas String Lights Wedding Party Garden Decor Light
03 Jan, 2018, 13:19:23 AEDST
beauty-sunshine15 (84877 )
Warm USB 100LED 10M Waterproof Christmas Fairy String Lights Xmas Wedding Garden
USB connector String Fairy Lights 10M 33ft 100 Led Copper Bottle Wedding Decor
Firework 100LED Copper Wire Strip String Fairy Lights Wedding Party Xmas Decor
Last updated on 22 Sep, 2018 14:44:34 AEST View all revisions
Fairies Round Party Balloons & Decorations,
Unbranded Wedding Fairy Tales Party Balloons & Decorations

value: This website is an American multinational e-commerce corporation based in San Jose, California, that facilitates consumer-to-consumer and business-to-consumer sales through its website.


text:
Prime Line U9802 White Sliding Window Lock - Walmart.com Skip To Primary Content Skip To Department Navigation Menu Toggle Search Walmart. Save Money. Live Better. Walmart. Save Money. Live Better. All All Departments Auto & Tires Baby Beauty Books Cell Phones Clothing Electronics Food Gifts & Registry Health Home Home Improvement Household Essentials Jewelry Movies Music Office Party & Occasions Patio & Garden Pets Pharmacy Photo Center Sports & Outdoors Toys Video Games Clear search field Search Hello, Hello. Sign In My Account Track My Order My Account Savings Catcher Compare prices and save Pharmacy Account Manage your prescriptions VUDU Library Unlimited access to thousands of movies and TV shows Sign Out Sign In View my account Create an Account Get more out of Walmart.com Items in cart Hello. Sign In Main Menu All Departments Halloween Costumes Electronics & Office Shop Electronics TV & Video Shop all TVs DVD & Blu-ray Players Home Audio & Theater Cell Phones Shop all No-Contract Phones Unlocked Phones Prepaid Minutes & Data Straight Talk Home Automation Wearable Tech Cut the Cable iPad & Tablets Shop all iPad Windows Tablets Android Tablets Accessories Computers Shop all Laptops Desktops Printers & Supplies Networking Software Monitors Accessories Auto Electronics Shop all GPS & Navigation Video Games Shop all Value Bundles Cameras & Camcorders Portable Audio Shop all iPod & MP3 Players Headphones Speakers & Docks Office Shop all Office Supplies School Supplies Small Business Center Furniture Office Technology Tips & Advice Movies, Music & Books Movies & TV Shop all Action & Adventure Blu-ray Discs Boxed Sets Children & Family Instawatch Movie DVDs New Releases Preorders TV Shows Video on Demand by VUDU Teenage Mutant Ninja Turtles Music Shop all Collections & Boxed Sets Country Music Music DVDs Music Gift Cards Musical Instruments New Releases Pop R&B / Soul Rock Ticketmaster Top 100 CDs Vinyl Books Shop all Adult Coloring Books Audiobooks Children's & Teen Books Classic Books Harry Potter Journals New Releases Romance Books Textbooks Top 200 Sellers Batman v Superman Captain America Star Wars Home, Furniture & Patio Shop All Home Pioneer Woman Better Homes and Gardens Bath Bedding Shop all Bedding Sets Basic Bedding Kids' Bedding Teen Bedding Home Decor Shop all Curtains & Blinds Lighting Rugs Furniture Shop all Bedroom Dining Room Kids' Furniture Living Room Mattresses Office TV Stands Kitchen & Dining Shop all Cookware, Bakeware & Tools Dining & Entertaining Kitchen Appliances Kitchen Storage Vacuums & Floor Care Patio & Garden Shop all Grills & Outdoor Cooking Patio Furniture Patio & Outdoor Decor Storage & Organization Appliances Luggage Featured Shops Shop all Back to College Personalized Gifts Kids' Rooms Teen Rooms Wedding Registry Style Tips & Ideas Home Improvement Shop All Home Improvement Tools Shop all Power Tools Hand Tools All Tool Sets Home Automation Generators Garage Storage Shop all Free Standing Shelves Garage Cabinets Baskets & Bins Garage Door Openers Bathroom Renovations Kitchen Renovations Heating & Cooling Shop all Air Conditioners Heaters Portable Fans Fireplaces Dehumidifiers Water Purification Light Bulbs Paint Shop all Wallpaper Patio & Garden Shop all Patio Furniture Patio Accessories Patio & Outdoor Décor Gardening & Lawn Shop all Outdoor Equipment Sheds & Outdoor Storage Landscaping Grills & Outdoor Cooking Garden & Patio Tips & Ideas Home Safety Hardware Clothing, Shoes & Jewelry Shop All Clothing Fall Fashion Girls' Clothing Boys' Clothing Baby & Kids' Shoes Baby & Toddlers' Clothing School Uniforms Backpacks Bags & Accessories Shoes Character Shop Women's Women's Plus Juniors' Intimates & Sleepwear Maternity Women's Shoes Handbags Jewelry Shop all Personalized Jewelry Wedding Rings Watches Fine Jewelry Fashion Jewelry Activewear Shop Men's Men's Big & Tall Young Men's Men's Shoes Men's Accessories Shop all Underwear Socks Sleepwear Sports Fan Shop Swim Shop Clearance & More Baby & Toddler Shop All Baby Baby Savings Best Sellers Baby Registry Nursery & Decor Shop all Furniture Bedding Decor Nursery Collections Toddler Shop all Furniture Bedding Decor Car Seats Strollers Activities & Gear Shop all Swings Playstations Bouncers Feeding Shop all Baby Formula Baby Food Breast Feeding Bottle Feeding Diapering Shop all Diapers Wipes Toys Shop all Learning Toys Ages 0-12 Months Ages 12-24 Months Health & Safety Shop all Baby Gates Baby Monitors Clothing Shop all Costumes Baby Girls (0-24M) Baby Boys (0-24M) Toddler Girls (2-5T) Toddler Boys (2-5T) Shoes Toys & Video Games Chosen by Kids Shop All Toys Shop all Dolls & Dollhouses LEGO & Building Sets Action Figures Cars, Trains, & Remote Control NERF & Blasters Pretend Play Arts & Crafts Games & Puzzles Musical Instruments Preschool Toys Shop all Learning Toys Star Wars Outdoor Play Shop all Swimming Pools Swing Sets Trampolines Waterslides Adult Bikes Bikes & Ride-Ons Shop all Kids' Bikes Ride-On Toys Scooters Boys' Toys Girls' Toys Toy Tips & Trends Video Games Shop all Accessories Bundles Digital Gaming New Releases Nintendo 3DS / 2DS Nintendo Wii U / Wii PlayStation 3 PlayStation 4 Preorders Used Games Xbox One Xbox 360 Savings Showcase Gaming Tips Food, Household & Pets Shop All Food Shop all Coffee Beverages Snacks & Cookies Breakfast & Cereal Meals & Pasta Candy & Gum Condiments & Spices Food Shops Shop all Organic Gluten Free Emergency Food Food Gifts Halloween Shop all Boo It Forward Baking Center Grocery Pickup & Delivery Shop All Pets Shop all Pet Food Dogs Cats Fish Birds Small Animals Household Essentials Shop all Laundry Room Kitchen Bathroom Paper & Plastic Cleaning Supplies Air Fresheners Batteries Pest Control As Seen On TV Pharmacy, Health & Beauty Pharmacy Shop all Transfer Prescriptions Refill Prescriptions History Medicine Cabinet Care Clinic Health Insurance Vision Center Shop all Contact Lenses Prescription Eyewear Funeral Personal Care Shop all Oral Care Incontinence Feminine Care Deodorants & Antiperspirants Shaving Health Shop all Cold, Cough, Flu Season Medicine Cabinet Diet & Nutrition Home Health Care Vitamins Sports Nutrition Diabetes Shop Beauty Shop all Bath & Body Fall Beauty Trends Cold weather Skin care Fragrances Massage & Spa Men's Grooming Subscribe to Beauty Box Hair Care Makeup Skin Care Sports, Fitness & Outdoors Shop All Sports Camping Shop all Tents Canopies, Gazebo's & Shelters Coolers Sleeping Bags Airbeds Fishing & Marine Shop all Rods Reels Rod & Reel Combo's Marine Accessories Boats & Water Sports Hunting & Shooting Shop all Gun Safes & Storage Game & Trail Cameras Tree Stands & Blinds Optics & Binoculars Archery Hunting Apparel Team Sports Shop all Baseball Softball Basketball Football Golf Bikes Exercise & Fitness Shop all Exercise Machines Treadmills Exercise Bikes Ellipticals Strength & Weight Training Fitness Accessories Recreation Trampolines Game Room Shop all Multi-Game Tables Air Hockey Fanshop Auto & Tires Auto & Tires Auto Parts Shop all Oil Filters Engine Air Filters Cabin Filters Car Lighting Auto Body Tools Shop all Auto Repair Tools Motor Oil & Lubricants Shop all Antifreeze & Coolants Motor Oil RV Motorcycle & ATV Car Safety & Security Shop all Anti-Theft Devices Tires Tire Finder Batteries & Accessories Shop all Car Batteries Car Battery Chargers & Accessories Auto Electronics Shop all In-Dash Stereos Auto Services Auto Tips & Advice Auto Detailing & Car Care Shop all Car Washes & Cleaners Car Wax & Polish Cleaning Tools Detailing Kits Pressure Washers Exterior Car Accessories Shop all Cargo Management Exterior Car Decoration Interior Car Accessories Shop all Floor Mats Seat Covers Photo, Gifts & Personalized Shop Photo Shop all Available Same Day Blankets Calendars Canvas & Wall Art Cards & Invitations Home Décor Mugs Office Phone Cases Photo Books Posters Prints Gift Registry Shop all Baby Registry Wedding Registry Lists Shop Photo Cards By Occasion Shop all Baby Birthday Thank You Wedding See All Cards As Seen On TV Food Gift Baskets 3D Printers Gift Cards Shop all Mail a Gift Card Email a Gift Card Specialty Gift Cards Check Card Balances Corporate Gift Card Program Personalized Gifts Shop all For Him For Her For Baby For Kids For the Couple Personalized Jewelry Halloween, Party & Sewing Halloween Shop all Women's Costumes Men's Costumes Boys' Costumes Girls' Costumes Baby & Toddler Costumes Costume Accessories Halloween Graphic Clothing Candy Shop all Trick or Treat Bags Halloween Decorations Shop all Inflatables Lighting & Effects Party & Occasions Shop all Baking & Pastry Supplies Disposable Tableware Giftwrap & Supplies Party Decorations Party Favors Baby Shower Birthday Supplies Christmas Wedding Shop All Party Themes Party Ideas & Recipes Gift Cards Sewing & Crafts Shop all Sewing Fabric Scrapbooking Supplies Art Supplies Beading & Jewelry Kids' Crafts Craft Storage Artificial Plants & Flowers Patterns Albums Stamping & Embossing Yarn Craft Tips & Ideas See All Departments See moreless Fall Fashion Trends My Local Store Pick It Up TODAY Tips & Ideas FREE Walmart Grocery pickup Learn how to save 10%, up to $25 FREE shipping on $50+ orders FREE store pickup as soon as today Previous Next
Home Improvement Windows Window Hardware Window Hardware Prime Line U9802 White Sliding Window Lock 0.0 stars 0.0 stars Write a review Write a review ratings Q&A By: Prime-Line Products About this item Heavy duty cast aluminum with single thumbscrewSecurely locks window in closed or ventilating positionEasy to install Read more… $4.94 $4.94 FREE shipping on orders $50+ Close If your order totals $50 or more and you select Value shipping, this item and any other qualifying items ship for free! Was: $9.08 Save $4.14 Sold & Shipped by Walmart FREE shipping on orders $50+ Get it by Oct 4, with Rush shipping Shipping options Pickup not available Pickup options Loading options... Sold & Shipped by Walmart Qty: 1 2 3 4 5 6 7 8 9 10 11 12 Add to Cart
Add to List Add to Registry Shipping See delivery options FREE pickup Not available FREE shipping available on $50+ orders Close Shipping Shipping options from Walmart Find We don't recognize that location. Check your information and try again. Type Arrives Cost Value Fri, Oct 7 $5.97 per order Standard Thu, Oct 6 $7.97 per order Expedited Wed, Oct 5 $6.36 per item Rush Tue, Oct 4 $10.21 per item You’ll see exact shipping costs and arrival dates when you check out. Pickup not available Close Pickup Find We can't find any pickup locations near the location you entered. Check your information and try again. Want to pick up your package for free? Walmart offers free pickup for most orders placed online - for many items as soon as today! Tell us where you are and we'll show you which Walmart stores and partner locations near you are available for package pickup. Add to List Add to Registry FREE shipping on orders $50+ Get it by Oct 4, with Rush shipping Shipping options Pickup not available Pickup options 4 other sellers from $5.91 $5.91 + Free shipping Sold & Shipped by SJS Commercial, LLC $2.92 + $5.32 shipping Sold & Shipped by Vanguard Wholesale Compare all 5 sellers Customers also viewed these products $4.77 Prime Line U9800 Aluminum Sliding Window Lock ShippingPass $4.67 Was $5.43 Save $0.76 Ultra Hardware 46103 Aluminum Sliding Door & Window Lock ShippingPass $7.20 Prime Line S4064 Sliding Cam Action Door & Window Lock ShippingPass $4.53 Was $6.44 Save $1.91 Ultra Hardware 48002 Aluminum Sliding Patio Door & Window Lock 2.0 stars 1 ratings ShippingPass Rollback $10.26 Was $12.49 Save $2.23 Ultra 44900-U Satin Finish Sliding Door Bar Locks 3.0 stars 2 ratings ShippingPass $5.23 Was $8.57 Save $3.34 Prime Line S4032 Sash Window Lock ShippingPass $4.94 Prime Line S4001 Black Slide Window Lock ShippingPass $6.86 Was $8.51 Save $1.65 Prime Line S4000 Mill Finish Slide Window Lock ShippingPass $5.08 Prime Line F2590 White Window Sash Lock ShippingPass $7.27 Was $8.91 Save $1.64 Prime Line S4018 Window Flip Lock ShippingPass $22.99 Was $40.89 Save $17.90 GE Wireless Alarm System Kit 4.0 stars 79 ratings ShippingPass $7.12 Ultra 44913 Aluminum Patio Door and Window Lock ShippingPass $9.97 GE 45115 Wireless Window Alarms, 2pk 4.5 stars 42 ratings ShippingPass $13.09 Sliding Door Bar Lock ShippingPass $5.10 Was $7.89 Save $2.79 Prime Line S4065 1-3/8" Sliding Window Pin ShippingPass $6.06 Sliding Window Lock ShippingPass $19.09 Master Lock 265DCCSEN Dual Function Security Bar 4.0 stars 10 ratings ShippingPass $7.20 Was $10.12 Save $2.92 Ultra Hardware 45000 Aluminum Sliding Patio Door Foot Lock ShippingPass $27.62 Trademark U.S. Patrol Alarm Security Bar 2.5 stars 16 ratings ShippingPass $9.36 Prime Line Products E2103 Sliding Glass Door Lock ShippingPass $16.16 Prime Line Products C1139 Sliding Glass Door Locking Handle ShippingPass Check out these related products Clearance $25.28 List price $30.34 Save $5.06 KIT WRENCH WINDOW LOCK ZINC ShippingPass $14.65 SASH LOCK WHITE ShippingPass $7.11 Was $7.50 Save $0.39 Prime Line Products H3553 Window Sash Lock Casement ShippingPass $9.58 Prime Line Products GD52112 Roller ShippingPass Rollback $5.73 Was $9.06 Save $3.33 Prime Line GD52203 Door Roller ShippingPass $4.30 Was $5.16 Save $0.86 Prime Line R7085 Drawer Guide Kit ShippingPass $12.74 Prime Line Products H3514 Left Hand Casement Operator ShippingPass $12.74 Prime Line Products H3520 Right Hand Casement Operator ShippingPass $7.92 Was $9.55 Save $1.63 Prime Line Products GD52113 Track Roller ShippingPass $7.56 Prime Line Products D1525 Roller Assembly ShippingPass $7.40 Was $7.97 Save $0.57 Prime Line Products N6548 Roller Assembly ShippingPass $5.13 Was $8.69 Save $3.56 Prime Line N6775 White Wardrobe Door Guide ShippingPass $5.30 Was $8.92 Save $3.62 Prime Line N6555 Bi-Pass Door Guides ShippingPass $3.90 Prime Line N6559 Wardrobe Bottom Door Guide ShippingPass $5.28 Prime Line N6661 Wardrobe Door Bottom Guide ShippingPass $8.74 Prime Line U10271 15/16" Extruded Aluminum Sliding Window Lock ShippingPass $4.26 Was $4.51 Save $0.25 Prime Line N6565 Bi-Pass Door Guide ShippingPass Reduced Price $4.21 List price $6.03 Save $1.82 Prime Line / Slide Co F2511 Rh Slide Window Cam Lat ShippingPass $13.25 Prime Line Products H3710 White Left Hand Casement Window Operators ShippingPass $8.59 Prime Line Products D1533 Sliding Glass Door Roller Assembly ShippingPass $6.76 Prime Line Products H3536 Aluminum Finish Window Handle Tee-Crank ShippingPass About this item Customer reviews Item recommendations Policies Back to top About this item Important Made in USA Origin Disclaimer: For certain items sold by Walmart on Walmart.com, the displayed country of origin
recommended that you rely on product packaging or manufacturer information. This hardware is durable enough to offer years of functionality! Prime Line U9802 White Sliding Window Lock:
Heavy duty cast aluminum with single thumbscrew Securely locks window in closed or ventilating position Easy to install No tools required Carded White
Specifications Color: White Fabric Content: 100% Multi Material: Multi Manufacturer Part Number: U9802 Model: U9802 Brand: Prime-Line Products Assembled Product Dimensions (L x W x H): 0.88 x 3.81 x 5.44 Inches Show Best seller ranking: #111,423 in Home Improvement #410 in Home Improvement Windows #377 in Home Improvement Windows Window Hardware Explore related products Window Locks Window Sash Lock Prime-line Products Window Hardware Prime Line Prod. Window Hardware First Watch Security Window Hardware National Guard Window Hardware Customer Reviews Write a review Be the first to review this item. Share your rating and review so that other customers can decide if this is the right item for them.
Customer Reviews | reviews | out of Customer Reviews Be the first to review this item Customer Q&A Back Ask a question Back to questions Questions & Answers 1 questions Ask a question Back to questions Sort by: Number of answers Most recent questions Most recent answers 1-1 of 1 questions Ask a question If you would like to share feedback with us about pricing, delivery or other customer service issues, please contact customer service directly. Question Q&A tips and guidelines Q&A Exchange Guidelines When writing your question or answer, please follow these guidelines: Do: Make sure your question is directly related to the product Do: Be specific and provide as many details as possible Do: Include the model number (if applicable) Do: Write your question in English Do Not: Include personal information (such as your full name) All submitted questions and answers are subject to the terms set forth in our Terms and Conditions We reserve the right not to post your question or answer if it contains any of the following: Any information not related to the actual merchandise or topic Obscenities, discriminatory language or other language not suitable for a public forum Advertisements or “spam” content Email addresses, physical addresses or other forms of contact information Critical or spiteful comments on other questions or answers posted on the page or their authors Any imagery that demonstrates incorrect use of the merchandise Anything that Walmart deems to be inappropriate, at Walmart's discretion Additional details (optional) Provide as many details as possible, including the model number (if applicable). Remember: Don’t include personal information (such as your full name). Nickname Nickname Send me an email when my question is answered Email address I agree to the Terms & Conditions Submit Cancel Preview Preview Question answers by an anonymous customer answers answers Last answer: Answer this question Cancel my answer Answer this question Submit Edit Cancel Thank you. Questions will be checked against our question guidelines and posted within five to seven business days. Back Q. how many in a package 0 answers by cjb 6/3/2013 0 answers Report 0 answers Last answer: Answer this question Cancel my answer Answer this question 1 1-1 of 1 questions See all Consider these popular products $12.95 ScreenMend Window Screen Repair Patch 4.0 stars 1 ratings ShippingPass $5.12 Prime Line U9823 1" White Extruded Aluminum Sliding Window Lock, 4 Count 5.0 stars 1 ratings ShippingPass $2.84 List price $7.99 Save $5.15 New York Wire Screen Installation Tool 5.0 stars 4 ratings ShippingPass $10.94 New York Wire Screen Frame Kit, 48" x 48" 3.5 stars 8 ratings ShippingPass $5.01 Prime Line U9819 1" Extruded Aluminum Sliding Window Lock, 4 Count ShippingPass $8.82 ScreenMend Window Screen Repair Roll ShippingPass $40.00 ALEKO 4' x 2' Window Awning Door Canopy, Blue 4.5 stars 3 ratings ShippingPass $8.47 New York Wire 48" x 84" Bright Aluminum Screen 4.5 stars 4 ratings ShippingPass $9.97 Camco RV Awning De-Flapper, 2 Pack 4.5 stars 20 ratings ShippingPass $4.67 Was $5.43 Save $0.76 Ultra Hardware 46103 Aluminum Sliding Door & Window Lock ShippingPass Clearance $4.65 List price $5.44 Save $0.79 Prime Line P8095 3" x 3" Gray Adhesive Backed Screen Repair Patches, 5 Count 4.5 stars 3 ratings ShippingPass $59.00 Aleko 6' x 2' Window Awning Door Canopy (12 sq. ft Coverage) ShippingPass $7.60 New York Wire 36" x 84" Extra Strength Screen ShippingPass $13.72 Prime Line H3709 9" White Right Hand Casement Operator ShippingPass $4.88 Lorell Recycled Plastic Cubicle Nameplate 4.0 stars 1 ratings ShippingPass $4.94 Was $9.08 Save $4.14 Prime Line U9802 White Sliding Window Lock ShippingPass $66.20 ALEKO 4' x 2' Window Awning Door Canopy, Multistripe Green ShippingPass $54.99 ALEKO 4' x 2' Window Awning Door Canopy, Sand Color ShippingPass $50.00 Was $55.00 Save $5.00 ALEKO 6' x 2' Window Awning Door Canopy, Blue ShippingPass $4.81 Was $5.66 Save $0.85 Prime Line B512 Sliding Screen Door Spring Tension Roller 4.0 stars 1 ratings ShippingPass $13.25 Prime Line H3708 9" White Left Hand Casement Operator ShippingPass Policies & Plans See any care plans, options and policies that may be associated with this product. Warranty Plan Walmart Product Care Plans A limited product warranty is included free for most items. With an optional Walmart Care Plan you can enhance the manufacturer's coverage from the date of purchase. Walmart Product Care Plans cover 100% of the cost for repair or replacement, including shipping charges for the exchange. We'll show you Care Plan options and pricing at checkout if applicable, or go to www.walmart.com/careplans for more information. Product warranty: Manufacturer Warranty See details Warranty Information: Manufacturer Warranty Did you buy this in a Walmart store? Get a warranty for it here. Gifting plans
Returns are easy at Walmart Not happy with a purchase? No problem. We've made returning items as easy as possible. And, most purchases can be returned for free. Learn more about our Returns Policy. Compute- app-11031939-23-140021385, DC- prod-dal4, ENV- prod-a, Ver- 48.0.2, SHA- e98469bb55c5959751e37e4211d7253bbbd97537, Log- 5818dec8-2409c-1577e1a0c29000 Site Information Be the first to save! Email Address Sign up Privacy Policy Stay connected Mobile apps Help Feedback MoneyCenter Privacy & Security CA Privacy Rights Terms of Use Walmart services See All Services Walmart MoneyCenter Walmart Credit Card Product Care Plans Walmart Pay Get to know us About Walmart.com Corporate Suppliers Marketplace Careers @WalmartLabs Walmart.com Terms of Use About Our Ads Affiliate Program CA Privacy Rights Privacy & Security Customer service Returns Policy Product Recalls Int’l Customers Tax Exempt Program Contact Us Feedback Store Pickup In the spotlight Expand Baby Cribs Strollers Diapers Baby Feeding Car Seats Expand Electronics Cut the Cable Computers TVs iPads & Tablets Expand Fitness Exercise Machines Vitamins Ellipticals Treadmills Fitbit Expand Keep Cool Air Conditioners Fans Swimming Pools Pool Toys Popular Products Expand International Sites Argentina Brazil Canada Chile China Japan Mexico United Kingdom © Walmart Stores, Inc. To ensure we’re able to help you as best we can, please include your reference number: Ref:

value: This website is an American multinational retail corporation that operates a chain of hypermarkets , discount department stores, and grocery stores from the United States, headquartered in Bentonville, Arkansas.


text:
INSTEN TUFF Hybrid Phone Case Cover for Apple iPhone 5/ 5S
Today: $8.99 - $16.99
INSTEN Leather Wallet/ Swivel Windshield Holder for Apple iPhone 5/ 5S
This is a BasAcc White with Chrome Hole Rear snap-on rubber-coated case for Apple® iPhone 5/ 5S. Protect your cell phone against bumps and scratches with this case.
Easy-to-grip rubber coated exterior
Color: White with Chrome Hole Rear
Model Number 802013
INSTEN TUFF eNUFF Hybrid Zebra Phone Case Cover for Apple iPhone 5/ 5S
Today: $5.99 - $8.49
Draco Durable Snap on Metal Ventare A Arctic Case Cover for Apple iPhone 5/ 5S
Insten Soft Silicone Skin Rubber Case Cover For Apple iPhone 5/ 5S
Insten Green Hard Snap-on Case Cover For Apple iPhone 5/ 5S
lilith97 Jan 17, 2013
happycostumer221 Jan 9, 2013
donz4ski Dec 13, 2012
frontrangers Dec 5, 2012
Yes here is the link: http://www.overstock.com/Electronics/BasAcc-White-Chrome-Hole-Rubber-Coated-Case-for-Apple-iPhone-4-4S/6658335/product.html


text:
The curious case of Justice Karnan and its implications for higher judiciary | india news | Hindustan Times
Calcutta high court judge, justice CS Karnan, has been issued a contempt notice by Supreme Court for sending a letter to the PM with a list of sitting and retired high court and SC judges whom he wants “interrogated” on the grounds of corruption.
india Updated: Mar 09, 2017 14:19 IST
For the first time, a sitting high court judge, Justice CS Karnan, was hauled up by the Supreme Court to answer a contempt charge.(HT file photo)
On February 8, for the first time ever, a sitting high court judge was hauled up before the Supreme Court to answer for a contempt charge. A seven-judge bench of the top court issued contempt notice to Justice CS Karnan of the Calcutta high court.
In response, Justice Karnan invoked his identity as a Dalit and issued a letter stating that “the characteristic of this order clearly shows the upper caste judges are taking law into their hands and misusing their judicial power by operating the same against a SC/ST Judge with mala fide intention of getting rid of him”.
In 2011, Karnan had called a press conference to accuse a fellow high court judge of caste discrimination on the ground that the judge who sat next to him “deliberately” touched him with his foot. In 2015, he interrupted arguments going on in another courtroom in the Madras HC regarding judicial appointments, demanding to be heard. In April 2015, he began suo motu contempt proceedings against the chief justice of the Madras high court, Sanjay Kishan Kaul, accusing the latter of harassing and belittling him because he was a Dalit and by giving him “insignificant and dummy” portfolios. The Supreme Court stayed the same.
Karnan then accused Kaul of corruption in February last year, following which the top court transferred him that month, which Karnan issued a stay order on. When a two-judge Supreme Court bench lifted his stay order, he asked the Chennai police to book a case against the two judges under the SC/ST (atrocities) Act. The top court threatened to haul him up for contempt for some of the statements that he made, but Karnan apologised saying that his “mental balance” was severely affected. He finally took charge at the Calcutta high court after a private meeting with then-Chief Justice of India TS Thakur.
This time around, Karnan has been hauled up for sending a letter to the Prime Minister with a list of sitting and retired high court and Supreme Court judges whom he wants “interrogated” by investigative agencies on the grounds of corruption.
A Pattern of Judicial Indiscipline
There has been a pattern to most of these incidents — he makes an allegation against fellow judges, no proof is offered and he doesn’t push it further, until the next incident and provocation. His judicial work has also stirred controversy — recently, he had a verbal altercation in open court with his fellow judge in the Calcutta high court where they differed in controversial circumstances over granting bail to the accused in the Kolkata flyover collapse case.
The Tumultuous Appointment Process
Karnan was appointed as an additional judge of the Madras high court in 2009, upon the recommendation of the collegium of judges headed by then-Chief Justice of India, KG Balakrishnan. Karnan’s name was, in fact, first recommended by then-chief justice of the Madras high court, AK Ganguly. These are the only undisputed facts about Karnan’s elevation to the Madras high court. As with other judicial appointments, there is no official record as usual of the basis on which he was considered fit for appointment, what criteria were examined and whether any material against his elevation was considered and rejected.
While the Constitution does not prescribe any criteria beyond 10 years of legal practice and citizenship for a lawyer to be appointed as a High Court judge, in practice it’s usually at least 15 years and someone with a reasonably well-established practice in the High Courts. (This is not always followed, as many successful lawyers do not want to give up a lucrative practice for harsh work conditions and worse government pay.)
Integrity Under the Scanner
Fresh trouble for Calcutta high court Justice CS Karnan

value: This website is an Indian English-language daily newspaper introduced and founded in 1924 by Sunder Singh Lyallpuri, founder-father of the Akali movement and the Shiromani Akali Dal in the Punjab Province. It played integral roles in the Indian independence movement as a nationalist daily.


text:
Psychobiological Approaches for Anxiety Disorders: Treatment Combination Strategies by Stefan G. Hofmann | NOOK Book (eBook) | Barnes & Noble®
Psychobiological Approaches for Anxiety Disorders presents a comprehensive overview of the latest empirical evidence and research results on combining pharmacological agents and CBT techniques for the treatment of anxiety disorders. The first book to focus on the issue of enhancing CBT with pharmacological agents Features chapters
Psychobiological Approaches for Anxiety Disorders: Treatment Combination Strategies available in
Paperback - $48.09
9781118342473

value: This website is an American bookseller. It is a Fortune 1000 company and the bookseller with the largest number of retail outlets in the United States.


text:
75,880 Philadelphia Home Improvement Pros
Kitchen & Bath Design / Sales / Installation Read More New Creation Carpentry, LLC
New Creation Carpentry is a fully licensed (NJ & PA) and insured, Christian owned and operated, Burlington County, NJ based company specializing in home remodeling. New Creation... Read More Kountry Kraft
In the past half century, Kountry Kraft has evolved from a small family-owned business into one of the leading custom kitchen and custom cabinet manufacturers in the country. We... Read More Cottage Industries, Inc
12 Reviews	(610) 293-9750	Wayne, PA, 19087
Cottage Industries is a full-service residential design and construction company. Our services include creative architectural design, sound structural engineering, efficient and... Read More Mitchells Woodworking & Design LLC
We are a family run business of 23 years. Specializing but not exclusive in traditional style cabinetry and furniture. From your doors to your drawers we make it all! From design... Read More Lenore Frances Interiors
2 Reviews	(856) 552-0096	Mt. Laurel, NJ, 08054
Lenore Frances Interiors is your professional guide and collaborator to make your traditional home embrace the comfort and convenience of modern living. With a high quality... Read More Design Intervention by Danielle, LLC
5 Reviews	(215) 679-6369	Barto, PA, 19504
5 Reviews	(484) 347-6059	Boyertown, PA, 19512
Home staging, professional organizer, and cleaner for residential and commercial clients. Located in the Boyertown PA area. Read More Denise Vogel Homes - Keller Williams Real Estate
10 Reviews	(215) 200-9989	Langhorne, PA, 19047
I am a Realtor in Beautiful Bucks County PA working with both Buyers and Sellers. I love working with 1st Time Home Buyers, Empy Nesters looking to Right Size as well as those... Read More Becker Home
Here at Becker Home, we cherish the relationships we have with our clients. The smile we see on their faces from seeing their new décor makes us fully vested in every unique,... Read More Custom Renovations
8 Reviews	(856) 437-5055	Haddonfield, NJ, 08033
Starting in Princeton, NJ the Fikaris family started a painting company. Over 40 years later and under the next generation of owners the company may have changed names but the... Read More Myers Constructs, Inc.
2 Reviews	(215) 438-6696	Philadelphia, PA, 19144
Myers Constructs is an award-winning design to build general contracting firm serving the greater Philadelphia metro region. Our specialty is providing custom residential... Read More Peter Danko Design, Inc.
Peter Danko is an artist, artisan, designer, and inventor. His work is in museum collections, including MOMA and the Smithsonian Institute. His work has also been the subject of... Read More TO Property Solutions
5 Reviews	(215) 479-7805	Green Lane, 18054
Fully Insured Kitchen and Bathroom Remodeling Services Read More Sue Giacomucci Interiors
4 Reviews	(610) 574-2399	West Chester, PA, 19380
Full service interior design and decorating. Read More List your business here for free - Learn More 2627282930313233343536Next Page Professionals in Philadelphia Metro Area Philadelphia · Allentown · Reading · Bethlehem · Levittown · Chester · East Norriton · Norristown · Drexel Hill · Easton · Springfield · Pottstown · King Of Prussia · West Chester · Lansdale · Willow Grove · West Norriton · Phoenixville · Horsham · Ardmore	· more...
· Coatesville · Emmaus · Broomall · Lansdowne · Darby · Croydon · Northampton · Bristol · Woodlyn · Ft Washington · Lafayette Hill · Thorndale · Sellersville · Langhorne · Leesport · Kennett Square · Shartlesville · Stockertown · Sumneytown · Catasauqua · Parker Ford · Plumsteadville · Cheyney · Sharon Hill · Morton · Ridley Park · Alburtis · Bally · Souderton · Gladwyne	Professional Categories in Philadelphia Philadelphia Home Contractors · Philadelphia Custom Design-build Firms · Philadelphia Interior Decorators · Philadelphia Design Studios · Philadelphia Residential Kitchen Designers · Philadelphia Kitchen & Bath Remodelers · Philadelphia Builders · Philadelphia Landscapers · Philadelphia Licensed Landscape Contractors · Philadelphia Tile Studios · Philadelphia Cabinets & Cabinetry · Philadelphia Furniture Makers · Philadelphia Wood Flooring Installer · Philadelphia Painters · Philadelphia Decks, Patios & Outdoor Enclosures · Philadelphia Pool Suppliers · Philadelphia Custom Woodworkers · Philadelphia Doors · Philadelphia Garage Doors · Philadelphia Custom Closet Designers	· more...
· Philadelphia Lighting · Philadelphia Custom Curtains · Philadelphia Windows · Philadelphia Residential Photographers · Philadelphia Roofing & Gutters · Philadelphia Siding & Exterior Contractors · Philadelphia Specialty Painting · Philadelphia Appliance Distributors · Philadelphia Bed Products · Philadelphia Hardware Supplies · Philadelphia Carpenters · Philadelphia Driveways & Paving · Philadelphia Electrical Contractors · Philadelphia Environmental Services & Restoration · Philadelphia Fencing & Gates · Philadelphia Custom Hearth · Philadelphia Nursery · Philadelphia HVAC Contractors · Philadelphia Sound System Design & Installation · Philadelphia Staging Companies · Philadelphia Ironwork · Philadelphia Nursery Decor · Philadelphia Custom Cabinets · Philadelphia Lawn & Sprinklers · Philadelphia Design Bloggers · Philadelphia Outdoor Audio/Visual · Philadelphia Outdoor Play Systems · Philadelphia Plumbing Contractors · Philadelphia Rubbish Removal · Philadelphia Septic Tanks and Systems · Philadelphia Solar Energy Contractors · Philadelphia Sport Courts · Philadelphia Staircases · Philadelphia Stone, Pavers & Concrete · Philadelphia Tree Services · Philadelphia Real Estate Agents · Philadelphia Upholstery · Philadelphia Wine Cellars	Professional Metro Areas

value: This website is an American website, online community and software for architecture; interior design and decorating; landscape design and home improvement. It was founded in 2009 and is based in Palo Alto, California.


text:
module - Custom Magento Extension Enable and Disable not working - Stack Overflow
When I click on Enable it does not enable the extension. I know the extension actually works b/c if I manually change my layout block theme it works. However, I don't want to do that. Can you please have a look at my code and let me know what I am doing wrong? I am assuming it has something to do with my block file not being right. P.S. if you see what is wrong and how to fix it could you also tell me how to set a CSS file for the extension as well if it is enabled?
Here are all of my files :)
<Module_Name><version>1.0.0</version></Module_Name>
<class>Module_Name_Block</class>
<file></file>
<module>Module_Name</module>
<frontName>modulename</frontName>
<title>Shipping Extension</title>
<module translate="label">
Custom Extensions
<modulename translate="label">
Shipping
<tab>module</tab>
<settings translate="label">
Settings
<enable translate="label">
<![CDATA[Enable or Disable this extension.]]>
</modulename >
class Module_Name_Helper_Data extends Mage_Core_Helper_Abstract
Block/Cart/Shipping.php
class Module_Name_Block_Cart_Shipping extends Mage_Checkout_Block_Cart_Shipping
if(Mage::getStoreConfig('modulename/settings/enable'))
$this->setTemplate('module/name/shipping.phtml');
magento module block adminhtml share|improve this question edited Jun 13 at 8:26
For checking boolean config data it's more appropriate to use Mage::getStoreConfigFlag() [link]. In this case, there's a hook to do just this purely in layout XML without the need to do a block class rewrite.
Configure a custom layout update file for your module, and in that file simply do the following:
<action method="setTemplate" block="checkout.cart.shipping" ifconfig="dropdownshipping/settings/enable">
<template>beckin/dropdownshipping/drop_down_shipping.phtml</template>
<action method="addCss" block="head" ifconfig="dropdownshipping/settings/enable">
<template>css/beckin/dropdownshipping.css</template>
As long as your module is also configured with <depends /> on the Mage_Checkout this layout XML update will be merged in after the core instruction, thus overriding the core template.
The only reason to take the approach which you have taken is to thoroughly force that the template will be set to your module's template just prior to rendering - thereby overriding any potential conflicting layout XML instruction - assuming that there is no cache hit, a behavior which is ... debatable.
share|improve this answer edited Dec 16 '12 at 18:10
answered Dec 16 '12 at 15:58
16.8k12048
Thank you @benmarks! So should I remove the shipping block that I have made and add this to the etc/config.xml file or to the template layout.xml file like the one I have commented out in the etc/config.xml file?
You are the man!! Thank you so much! I did not know you could control that with the layout by adding the ifconfig="dropdownshipping/settings/enable". You rock! Thanks so much!!

value: This website is a question and answer website for professional and enthusiast programmers. It is the flagship site of the Stack Exchange Network, created in 2008 by Jeff Atwood and Joel Spolsky.


text:
c 1930s BUNGAY SUFFOLK ST MARYS STREET SCENE POSTCARD RPPC | eBay Please enable JavaScript Our new search experience requires JavaScript to be enabled. Please enable JavaScript on your browser, then try again.
View: Gallery view c 1930s BUNGAY SUFFOLK ST MARYS STREET SCENE POST...: Items found similar to "c 1930s BUNGAY SUFFOLK ST MARYS STREET SCENE POSTCARD RPPC"
1930s CRIPPLE CREEK COLORADO RPPC Street Scene autos postcard 12117
Nome Alaska AK Snow Street Scene Milos Cafe Real Photo Postcard RPPC c1930-50
c. 1930 The Main Street View Scene Haworth West Yorkshire England RPPC Postcard
RPPC JUNEAU, Alaska AK FRANKLIN STREET Scene ca 1930s Postcard
Walla Walla Washington WA St Marys Hospital Real Photo Postcard RPPC c1930-50
RPPC BIG FLATS, New York NY CANAL STREET Scene ca 1930s-40s Postcard
RPPC NUEVO LAREDO, MEXICO View of LAREDO, TEXAS Street Scene 1930s Postcard
RPPC POPOCATEPETL , Mexico Dirt Street Scene Street Scene c1940s Postcard
1st Street Scene C-1908 Steamship Company CORDOVA ALAKSA RPPC postcard 11351
RPPC Postcard Patriotic Scene Main Street American Flags Old Cars c. 1900s
VINTAGE RPPC POSTCARD RIVER SCENE ELEPHANTS COLOMBO CEYLON 1930`S (G12)
State Capitol & Bird's Eye View St. Paul 1930s Street Scene Old Cars postcard $7.99
C-1920s Noll Street Scene RUPERT IDAHO RPPC postcard 9798
RPPC, HARBIN HOT SPRINGS, California CA WINTER SCENE SNOW c1930's Postcard
RPPC GEORGIA ST., VALLEGO, CA BUSY STREET VIEW MANY SIGNS 1930-40'S AUTOS $15.00
RPPC LAKE TAHOE, California CA SNOWY SCENE c1930s-40s Postcard
RPPC FAIRBANKS, Alaska AK SECOND AVE. Street Scene Cars 1930s-40s Postcard
"C" Street, Virginia City, NV Photograph Postcard RPPC by Frasher - 1930's
Dock Street KETCHIKAN ALASKA Real Photo Postcard - Street Scene 1930-40s RPPC
c-1910 ROCKFORD ILLINOIS Street Scene Clothing Store RPPC postcard 100894
RPPC – Street Scene in Gila Bend Arizona c1930-40s – Pima Street
1930's Winter Scene on US 40 in SIERRA CALIFORNIA RPPC postcard 6234
RPPC LIVINGSTON, Montana MT CALENDAR STREET Scene c1910s Postcard
C-1910 MAPLE PLAIN MINNESOTA Street Scene RPPC Real Photo postcard 13051
RPPC Chinatown San Francisco California 1930's Street Scene $12.00
RPPC ST. JOHNS, MI STREET SCENE 1940s Cars State Bank of St. Johns Postcard
RPPC, SAN FRANCISCO, CA California CALIFORNIA STREET c1930's Postcard
STREET SCENE Potinger St. Vintage RPPC Real Photo POSTCARD Hong Kong China