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Patent US4666565 - Gas sensor and method - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsAn electrochemical cell for use in the detection and measurement of water soluble gases includes a vessel having a first chamber adapted to receive and contain said gas and having located therein a working electrode and a second chamber adapted to contain an electrolyte and having located therein a reference...http://www.google.com/patents/US4666565?utm_source=gb-gplus-sharePatent US4666565 - Gas sensor and methodAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS4666565 APublication typeGrantApplication numberUS 06/884,882Publication dateMay 19, 1987Filing dateJul 10, 1986Priority dateApr 28, 1983Fee statusPaidAlso published asEP0125822A2, EP0125822A3Publication number06884882, 884882, US 4666565 A, US 4666565A, US-A-4666565, US4666565 A, US4666565AInventorsJohn V. DobsonOriginal AssigneeBritish Gas CorporationExport CitationBiBTeX, EndNote, RefManPatent Citations (9), Referenced by (9), Classifications (11), Legal Events (8) External Links: USPTO, USPTO Assignment, EspacenetGas sensor and method
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS3755125 *Jan 14, 1971Aug 28, 1973Envirometrics IncElectrochemical gas analyzerUS4394239 *Aug 24, 1981Jul 19, 1983Bayer AktiengesellschaftElectro-chemical sensor for the detection of reducing gases, in particular carbon monoxide, hydrazine and hydrogen in airUS4406770 *Feb 1, 1982Sep 27, 1983Coal Industry (Patents) LimitedGas sensorGB1372245A * Title not availableGB1433071A * Title not availableGB1473250A * Title not availableGB1484886A * Title not availableGB2075197A * Title not availableGB2094005A * Title not available* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS4769122 *Jul 10, 1987Sep 6, 1988Bacharach, Inc.Compact electrochemical cell for gas detectionUS5527446 *Apr 13, 1995Jun 18, 1996United States Of America As Represented By The Secretary Of The Air ForceGas sensorUS5763763 *Oct 15, 1996Jun 9, 1998Ngk Insulators, Ltd.Method and sensing device for measuring predetermined gas component in measurement gasUS6821340 *May 31, 2001Nov 23, 2004Hoya CorporationMethod of manufacturing silicon carbide, silicon carbide, composite material, and semiconductor elementUS20100300899 *Jun 21, 2010Dec 2, 2010The Trustees Of Columbia University In The City Of New YorkActive CMOS Sensor Array For Electrochemical Biomolecular DetectionEP1959253A3 *Mar 2, 1997Sep 9, 2009Mine Safety Appliances CompanyElectrochemical sensorWO1996025662A1 *Feb 13, 1996Aug 22, 1996M Squared Technology LimitedAqueous liquid analysisWO2009082706A1 *Dec 19, 2008Jul 2, 2009The Trustees Of Columbia University In The City Of New YorkActive cmos sensor array for electrochemical biomolecular detectionWO2015124276A1 *Feb 11, 2015Aug 27, 2015Dräger Safety AG & Co. KGaAElectrochemical gas sensor* Cited by examinerClassifications U.S. Classification205/780.5, 204/412, 204/431, 205/782, 204/432International ClassificationG01N27/416, G01N27/49Cooperative ClassificationG01N27/4045, G01N27/404European ClassificationG01N27/404, G01N27/404BLegal EventsDateCodeEventDescriptionMar 9, 1988ASAssignmentOwner name: BRITISH GAS PLC, RIVERMILL HOUSE 152 GROSVENOR ROAFree format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:BRITISH GAS CORPORATION;REEL/FRAME:004859/0891Effective date: 19870512Owner name: BRITISH GAS PLC, ENGLANDFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BRITISH GAS CORPORATION;REEL/FRAME:004859/0891Effective date: 19870512Dec 18, 1990REMIMaintenance fee reminder mailedMay 19, 1991REINReinstatement after maintenance fee payment confirmedJul 30, 1991FPExpired due to failure to pay maintenance feeEffective date: 19910519Oct 22, 1991FPAYFee paymentYear of fee payment: 4Oct 22, 1991SULPSurcharge for late paymentJan 21, 1992DPNotification of acceptance of delayed payment of maintenance feeSep 26, 1994FPAYFee paymentYear of fee payment: 8RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services


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Jury hears closing arguments in trial of Georgia man charged with killing 8 in mobile home | Fox News
BRUNSWICK, Ga. – Jurors are hearing attorneys' closing arguments in the trial of a Georgia man charged with clubbing his father and seven extended family members to death inside their mobile home four years ago. Defense attorney Newell Hamilton Jr. asked the jury Wednesday to acquit 26-year-old Guy Heinze Jr. of Brunswick. He said waffling by prosecutors on whether they believe Heinze committed all eight killings by himself — something police have said for four years — shows that authorities "still don't know what happened." Hamilton also said the motive given by police that Heinze killed the victims Aug. 29, 2009, over a bottle of prescription painkillers makes little sense. Prosecutors are seeking the death penalty for Heinze. They are scheduled to make their closing argument Wednesday after the defense finishes. Advertisement

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ByDPon October 30, 2015
Roses are Red by James Patterson. Well now, Detective Cross is once again in the middle of a series of murders and bank robberies. There seems to be no clues to be found any where. Why? Cross teams up with FBI agents, Kyle Craig, Betesy Cavalierre and two other agents.Together the three are traveling from Washington D.C. to New York try to put together just how this group of killers knows just what is what in the banking industry. People taken hostage and then released without harm while others are murdered in cold blood. A thrilling read indeed. See if you can come up with your own ideas as to who is the "mastermind!" See if you know just a little bit of what is going to happen to the three main characters; that is Cross, Cavalierre and Craig. I got a gut feeling about one hundred pages before the end of this well written book. Buy it! You'll like it! Rated PG. Some strong language and sexual content. DP, Castro Valley, CA.
2.0 out of 5 starsBoring and Unsettling
ByTUCSON ROBERTon October 26, 2013
ROSES ARE RED by James Patterson features crack Washington DC homicide detective Alex Cross.
I've read almost 40 James Patterson books including at least a half dozen from the Alex Cross series. I must say in comparison, Roses never climbed above the mediocre level. Patterson's characters in this writing lack depth. Even Cross's FBI love interest, Betsey Cavalierre, who could have been a prime contributor to the story, appeared more like a one-night stand than a thoughtful love interest. The writer's constant return to Cross's family with Nana Mama and the boring Cross clan was distracting and more a diversion than a part of the story.
The ending leaves the reader hanging. Appearing as an afterthought, Patterson pulled a villain out of thin air in the last couple pages leaving the reader aghast and cheated. Is there to be a follow on, a sequel? Good question.
I was disappointed in the book. I give Roses no more than a 2 rating out of a possible 5.
Showing 1-10 of 74 reviews(2 star). See all 909 reviews
BySean Murphyon June 18, 2013
This was my first James Patterson novel. I liked it okay in the beginning and middle, but the end turned me off to James Patterson. Roses are Red is an Alex Cross novel with the police psychologist pitted against a "mastermind" criminal. When I thought the climax of the novel would pit Alex against the mastermind, I enjoyed it. But the end had two too many plot twists which threw all the set-up in the middle of the novel out the window. It was very unsatisfying.
The characters were pretty thin, even the main one, Alex Cross. He had personal conflicts (friction with his girlfriend and his child had an illness) but they were not tied well into the main conflict of finding the mastermind, so they were more distraction than addition to the suspense.
Even though it was easy to read and enough of a page-turner to get through it quickly, in the end it was completely forgettable.
2.0 out of 5 starslike violets are blue actual content is ok
Bya.andersonon January 3, 2017
like violets are blue actual content is ok, but the quality of the actual book is poor in the extreme, also the clarity of the print what we have here is a cheap nasty copy of a geat book, bought for an above average price, delivering an absolutely inferior item not really worthy of being linked with amazon!!! you should ditch this outfit immediately
2.0 out of 5 starsforces you to buy another book- not worth the slog
ByRebeccaon November 28, 2014
This was a slog. The story felt very templated. Introduce a character, blow a few sentences of life into them and move on. For me the characters didn't make sense most of the time. I think it's because they were only devices to move the plot. Just so you know- you have to buy the next book to see how the whole thing plays out. I didn't care enough to bother.
2.0 out of 5 starsRoses are Red
Byminimoonon June 9, 2014
Didn't keep my attention as well as some if the others but not a bad read. Sadly, Patterson - ONCE AGAIN - has followed his usual trend in his choice for bad guy. I predicted this 3 books ago and was pleased to be wrong. Now so disappointed to find out I was right all along. Mysteries should NOT be so predictable. I'll try the next book, but at this rate, I might give up on this series if each book keeps sticking to the exact same "shock" at the end. It's never a shock any more. Getting incredibly old!
2.0 out of 5 starsRECEIVED USED MASS PAPERBACK INSTEAD OF NEW PAPERBACK COPY!
ByA. Hillon August 12, 2013
If melissa_fox is going to continue selling books on Amazon.com, she needs to learn the difference between mass paperback and paperback and the difference between new and used. I purchased this book for $3.98 as a new paperback copy. I received a used mass paperback copy. I can see that pages show signes of being dog eared. I think $3.98 is a very high price for a used mass paperback book. Will not use this person again. Very disappointed.
2.0 out of 5 starsStopped in the middle.
ByPatti Phxon April 15, 2016
James Patterson is a brilliant writer & a shameless con man. Writing half a story to sell one more book. I'm not falling for it & I won't buy "Violets are blue"
2.0 out of 5 starsQuick read, but not up to Patterson par
I got this book and finished it in a total of three and a half hours. Although his books are usually a quick read, I became bored with the plot halfway through. Hopefully he will provide criminal motivation and method of thinking in the next book, because the criminal seems totally out of character here. I still haven't decided if I'll read the next one.
2.0 out of 5 starsThis one did it - No more James Patterson for me.....
ByLBon December 9, 2000
I read through the other reviews of this book. Apparently many readers agree with me. We have given James Patterson and Alex Cross many, many chances. I was on the edge of giving up on these books after reading the last Alex Cross. But because of the fine quality of earlier books, I plunged into this last one, Roses are Red. Never again.
It's just the same old thing over and over. A weird serial killer has a personal vendetta against Alex. I could even take another good book about that. But here's the real problem that has displayed itself in so many of the past several books - there is no solution or conclusion to the story lines.
The killer from either 3 or 4 books ago is still around. Alex thought he had gotten him, but it is revealed that he has just killed someone else. So, first of all, we are told that the criminal from a previous case has never really been captured. The story of the relationship between Alex and Christine is also just left hanging. If I was not familiar with the Alex/Christine story from other books, the description of what happens between them would make no sense at all.
Then, in this current book, there are several captures made of people who are thought to be the murderer. Every time a capture is made, the person says "You have got the wrong man". Of course, this being an Alex Cross book, it always is truly the wrong man because the real perpetrator is rarely caught in these stories. He is always free to roam to become the focus of a sequel. The final few sentences of the book supposedly do finally tell who the killer is. But, in my opinion, it was such an unbelievably dumb choice after reading the entire book, it didn't satisfy my curiosity. It just made me mad. And, even this dumb choice of a serial killer is not caught.
After I have invested my time in reading a book, any book, I feel that I deserve a conclusion to most of the plot lines in the story. I do understand about sequels and that sometimes things need to be left hanging to keep the reader interested until the next installment. But these Alex Cross books go too far. The plots are never wrapped up. There is way too much left unsaid. I feel like I have been jerked around by James Patterson. If you lure me into the story, give me some satisfaction by the end of the book.
Maybe in some future book, James Patterson will take all of these loose threads and weave them into one huge and final conclusion. Even if he does, I will not be reading about it. This book was really my last Alex Cross.
2.0 out of 5 starsPart One of the two part Mastermind series, concluding with Violets are Blue.
ByScrapple8on September 24, 2016
Roses are Red by James Patterson is the 6th book in his Alex Cross series, and the first part of the two-part Mastermind episode that concludes with Violets are Blue. In this novel the Mastermind organizes three bank robberies, then a kidnapping on the families of insurance executives that mount to $30 million in ransom. Cross and the FBI team that he is working with capture two men who seem to be behind the crimes, but both tell Cross that he has the wrong guy.
The novel was ruined for me in two ways. I read Violets are Blue before this one, which ruined the big surprise in the final chapter of this novel. Alternatively, one could say that the final chapter of this novel ruined the reveal in Violets are Blue. The other problem was that the author made an error in telling the story. On page 76 of the novel, special agent Betsey Cavalierfe said, “First Union, Chase, First Virginia, and Citibank are all connected to us for the time being.” The novel had opened with the Citibank robbery in Silver Springs, MD. The Mastermind murdered his accomplices for this robbery, the Parkers, and hired new ones for his next job, at the First Union branch in Falls Church. A second pair of robbers handled the Falls Church robbery and a later one at the First Virginia robbery in Rosslyn.
Cavaliere made her statement during the investigation of the First Virginia robbery. At that time, no incident had been described at Chase. The statement was cleared up on page 129, when Chapter 44 began with the statement, “A robbery was in progress at the Chase Manhattan branch near the Omni Shoreham hotel in Washington.” This was the Chase robbery that Betsey Cavaliere mentioned on page 76.
The only problem was that the Chase robbery hadn’t happened when Ms. Cavaliere made that statement.
In Part 3, the Mastermind moved on to kidnapping the family members of an insurance company for ransom, so the error didn’t materially impact the story. Nevertheless, the error conveyed sloppiness and bothered me.
While Cross tried to solve the crimes, he dealt with a pair of personal problems that in themselves would affect anyone. In the previous novel, Pop Goes the Weasel, Geoffrey Shafer kidnapped the fiance of Cross, Christine Johnson. Christine had difficulty trying to put the traumatic incident behind her, especially after a bulletin from London announced that Lucy Rhys-Cousins, the wife of Shafer, was murdered in front of her children. Furthermore, Alex’s daughter Jannie has epileptic seizures caused by a tumor in her brain.
Agent Betsey Cavalierre senses that Alex is trying to juggle a complicated personal life with trying to solve the Mastermind crimes, and offers comfort “without complications.” Yet everyone knows that office romances involve complications, even if both parties come with eyes wide open. Alex also had a relationship with an FBI agent in Along Came a Spider, and that turned out badly. Killing off the character is one way to eliminate the complications.
The story wasn’t bad, and it is not Patterson’s fault that I read Violets are Blue before reading this story. But Patterson did screw up the story on page 76.

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Gra Polowanie 3 | eBay Please enable JavaScript Our new search experience requires JavaScript to be enabled. Please enable JavaScript on your browser, then try again.
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Patent US4368571 - Sleeving method - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsThe sleeving method comprises inserting a metal sleeve within a tube so as to bridge the defective region of the tube. The sleeve is constructed to have teeth on the outside thereof that are capable of tightly contacting the inside surface of the defective tube and creating a labyrinth-type seal therebetween....http://www.google.com/patents/US4368571?utm_source=gb-gplus-sharePatent US4368571 - Sleeving methodAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS4368571 APublication typeGrantApplication numberUS 06/185,656Publication dateJan 18, 1983Filing dateSep 9, 1980Priority dateSep 9, 1980Also published asDE3163395D1, EP0047407A1, EP0047407B1Publication number06185656, 185656, US 4368571 A, US 4368571A, US-A-4368571, US4368571 A, US4368571AInventorsFrank W. Cooper, Jr.Original AssigneeWestinghouse Electric Corp.Export CitationBiBTeX, EndNote, RefManPatent Citations (6), Referenced by (131), Classifications (18), Legal Events (1) External Links: USPTO, USPTO Assignment, EspacenetSleeving method
US 4368571 AAbstract
1. A method of securing a metal sleeve within a metal tube comprising:positioning in said tube a metal sleeve having annular grooves around a portion of the outside surface thereof; internally expanding the portion of said sleeve wherein said grooves are located thereby securing one end of said sleeve to said tube; securing the other end of said sleeve to said tube; and internally expanding the remainder of said sleeve into contact with said tube whereby said sleeve tends to shrink axially causing said sleeve to closely contact said tube establishing a substantially leak tight joint between said sleeve and said tube. 2. The method according to claim 1 wherein said step of securing said other end of said sleeve to said tube comprises internally expanding said other end of said tube wherein said annular grooves are located thereby securing said other end of said sleeve to said tube.
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Gesellschaft für Emissionstechnologie mbHMethod for mounting driving elements on a hollow shaftEP0291938A1 *May 18, 1988Nov 23, 1988Emitec Gesellschaft für Emissionstechnologie mbHApparatus for hydraulic expansionEP0293652A1 *May 11, 1988Dec 7, 1988Emitec Gesellschaft für Emissionstechnologie mbHExpension mandrel having a small diameter and a great lengthEP0780656A3 *Dec 5, 1996Nov 18, 1998Balcke-Dürr GmbHHeat exchangerWO1985004701A1 *Apr 1, 1985Oct 24, 1985Raychem CorportionPipe repair sleeve apparatus and method of repairing a damaged pipeWO1988009233A1 *May 20, 1988Dec 1, 1988Emitec, Gesellschaft Für Emissionstechnologie MbhDevice for hydraulic expansion* Cited by examinerClassifications U.S. Classification29/421.1, 138/98, 29/890.031, 29/523, 138/97International ClassificationB25B27/06, B21D39/20, F16L55/163, F28F11/02Cooperative ClassificationF28F11/02, F16L55/163, Y10T29/49805, Y10T29/4994, B21D39/203, Y10T29/49352European ClassificationB21D39/20B, F28F11/02, F16L55/163Legal EventsDateCodeEventDescriptionJun 24, 1999ASAssignmentOwner name: WESTINGHOUSE ELECTRIC CO. LLC, PENNSYLVANIAFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:CBS CORPORATION (FORMERLY KNOWN AS WESTINGHOUSE ELECTRIC CORPORATION;REEL/FRAME:010070/0819Effective date: 19990322RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services

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text:
Took this photo myself : S10wallpapers
u/yntnbbkv14
Took this photo myself
Shot on a smartphone or something else?
yntnbbkv14
Original Poster34 points · 28 days ago
Shot on my Galaxy S10
Wow that's so good.
Nice work on the positioning
was it a coincidence that the hole part was black?
Actually no. This photo is part of a larger photo. I just zoomed in a little so that the black hole can fit. If you look at the S10+ photo I posted, the photo is different because I zoom to another piece of that same photo to get me a wider lens cut out.
fani1996
Do you have a link to the original photo?
Looks like the hostas I took a picture of last night!
uravgconsum3r
Would you mind msging me how people do this? Meaning tag an image to their text turning it into a link I cannot figure it out.
I did it on mobile with the boost app. If you hit reply then click advanced, there is a button at the bottom that looks like a chain link. Click that, and it'll give you a place to paste the link and a place for what words you want it linked to. OR you could type it out like in the screenshot and add a link to whatever you want!
So download an app called boost?
Where do I get the link tho, do I need imgur post the pic to imgur then copy the link?
Sorry it took so long to respond, but if you press the camera icon it'll let you upload it from the app. And I only know how to do it with Boost, so if you want to get it with another app I can't help ya there lol
It's okay I didn't like the app but really appreciate you responding and trying to help me thank you!
terribletyrunt
Gorgeous! Thank you for posting!
dave-the-brave
Lol no. It's just some plant I saw on one of my job sites
Maybe it's wild garlic 🤯
jjisaman
Ilackcreativity_
Damn good photo. Thanks OP!
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text:
Computer World - Richmond, VA | Groupon
+18043596943
Computer World is surrounded by endless parking options.
4615 W Broad St, Richmond, VA 23230
http://www.computerworldrva.com
Church Hill (5.4 mi)
Groupon has verified that the customer actually visited Computer World.

value: This website is an American global e-commerce marketplace connecting subscribers with local merchants by offering activities, travel, goods and services in 15 countries. Based in Chicago, Groupon was launched there in November 2008, launching soon after in Boston, New York City and Toronto.


text:
Courtesy of the Atlanta Journal Constitution, Malzahn spoke of the season's in which the team travels to both rival stadiums: "That's as big of a challenge as anybody has in college football, in the years you play them on the road. The thing about it is you know it going into it, too. You just have to prepare the best you can from a coach's standpoint to deal with that. Like I said before, this is a place where we're going to have a top-10 schedule every year and you know that before the season. You've got to deal with it."
Fortunately for Malzahn, Auburn gets both their SEC rivals at home this season. They had better make the most of the break in their schedule, as 2016 swings back in favor of Auburn's most heated rivals.


text:
Patent US7024460 - Service-based compression of content within a network communication system - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsA service module incorporated within the network infrastructure intercepts packets communicated between a client and a server to determine whether the connection corresponds to an email service. If so, the service module breaks the connection by terminating the connection with the client at the service...http://www.google.com/patents/US7024460?utm_source=gb-gplus-sharePatent US7024460 - Service-based compression of content within a network communication systemAdvanced Patent SearchPublication numberUS7024460 B2Publication typeGrantApplication numberUS 10/095,551Publication dateApr 4, 2006Filing dateMar 11, 2002Priority dateJul 31, 2001Fee statusPaidAlso published asUS20030028606Publication number095551, 10095551, US 7024460 B2, US 7024460B2, US-B2-7024460, US7024460 B2, US7024460B2InventorsChris Koopmas, Constantine Polychronopoulos, Nicholas StavrakosOriginal AssigneeBytemobile, Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (8), Non-Patent Citations (5), Referenced by (32), Classifications (21), Legal Events (8) External Links: USPTO, USPTO Assignment, EspacenetService-based compression of content within a network communication systemUS 7024460 B2Abstract A service module incorporated within the network infrastructure intercepts packets communicated between a client and a server to determine whether the connection corresponds to an email service. If so, the service module breaks the connection by terminating the connection with the client at the service module and opening a separate connection between the service module and the server. Packets communicated between the client and the server may then be redirected to an email compression application that monitors messages communicated between the client and the server and processes the messages in accordance with the state of the email session. For messages corresponding to connection establishment, user authentication and other protocol-specific messages, for example, the email compression application may be configured to forward the messages to the originally intended destination. Messages corresponding to an email message data, however, are buffered within the email compression application. Once the entire message has been received, the email compression application may strip the message headers and any protocol-specific data, compress the data and attach new message headers corresponding to the compressed email message. The compressed and reformatted email message is then reinserted into the data stream for transmission to the intended destination. Because compression may occur between the server and client, compression may be performed without requiring special processing by the server before email messages are sent. Furthermore, because the email messages may be compressed in a format that can be readily decompressed using decompression libraries incorporated within the operating system of client devices, such as the CAB format or GZIP format, the client may decompress received email messages utilizing software already incorporated within the operating system of the client device, without requiring download or installation of special decompression software and/or coordination of compression/decompression of email messages with the server or sending party.
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2013Qualcomm IncorporatedMethods and apparatus for communicating transmission backlog informationUS8503938Jul 14, 2006Aug 6, 2013Qualcomm IncorporatedMethods and apparatus for determining, communicating and using information including loading factors which can be used for interference control purposesUS8514692Oct 14, 2005Aug 20, 2013Qualcomm IncorporatedMethods and apparatus for determining, communicating and using information which can be used for interference control purposesUS8514771Jan 17, 2006Aug 20, 2013Qualcomm IncorporatedMethods and apparatus for communicating and/or using transmission power informationUS8520511 *Sep 11, 2003Aug 27, 2013Qualcomm IncorporatedAutomatic handling of incoming communications at a wireless deviceUS8539513 *Mar 24, 2009Sep 17, 2013Alacritech, Inc.Accelerating data transfer in a virtual computer system with tightly coupled TCP connectionsUS8621013 *May 25, 2010Dec 31, 2013Hitachi, Ltd.Mail relay serverUS20100306329 *May 25, 2010Dec 2, 2010Masafumi KinoshitaMail relay serverUS20110004690 *Dec 3, 2008Jan 6, 2011ThalesMethod of Forwarding Messages Over a Network and System for Implementing the MethodUS20130133045 *Aug 30, 2012May 23, 2013Blackridge Technology Holdings, Inc.Method for directing requests to trusted resources* Cited by examinerClassifications U.S. Classification709/206, 709/207, 709/247International ClassificationG06F15/16, H04L12/58, H04L29/06, H04L29/08Cooperative ClassificationH04L67/2819, H04L67/2814, H04L67/28, H04L69/329, H04L69/04, H04L12/5895, H04L12/5835, H04L51/066European ClassificationH04L51/06B, H04L29/08N27, H04L29/08A7, H04L12/58C2, H04L29/06C5, H04L29/08N27ELegal EventsDateCodeEventDescriptionDec 6, 2013ASAssignmentOwner name: CITRIX SYSTEMS, INC., FLORIDAFree format text: NUNC PRO TUNC ASSIGNMENT;ASSIGNOR:BYTEMOBILE, INC.;REEL/FRAME:031730/0322Effective date: 20131203Sep 17, 2013ASAssignmentFree format text: THIS SUBMISSION IS TO CORRECT THE SPELLING OF THE RECEIVING PARTY (ASSIGNEE) S STREET NAME IN 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Day 6 Spring: TSD TV Part 1 - Lsu - Scout
Day 6 Spring: TSD TV Part 1Sonny Shipp03/09/2010LSU kicked off the second week of spring practice on Tuesday as the Tigers went through their sixth practice of the spring. Here is part one of a two-part series of video from Tuesday's session brought to you by TSD TV.Note: Due to technical difficulties there is no sound with this video.
Share on Facebook Share on TwitterTrending on ScoutFive Questions LSU must answer stillTiger Blitz Podcast Oct. 26What you need to know about ACC hoopsLSU OL commit honored to be a TigerLSU Football Offensive Bye Week Gradebook5-Star 'Bama Pledge Visits Another SEC School Full StoryTiger Blitz Top StoriesFive Questions LSU must answer stillThe Tigers certainly have already answered a lot of questions this team had following the Auburn loss, but there’s still some questions that the Tigers have to answer to achieve…by Billy EmbodyTiger Blitz1:00 AMLSU Football Offensive Bye Week GradebookTiger Blitz hands out grades for the first half of the season for the LSU Tigers on offense as they enjoy their bye week.by Billy EmbodyTiger BlitzYesterday at 6:45 AMLSU OL commit honored to be a TigerTiger Blitz was in DeSoto, Tex. to check in on 2017 LSU offensive line commit Ed Ingram and to get his thoughts on his senior season and LSU's impressive run.by Billy EmbodyTiger BlitzYesterday at 3:55 AMTiger Blitz Podcast Oct. 26Inside this week's Tiger Blitz Podcast: Will Leonard Fournette make it to New York for the Heisman? Sharing predictions for the rest of the season. What are the worries for LSU and…by Billy EmbodyTiger BlitzYesterday at 3:11 AMNew Tiger Blitz members receive a great perk!Another great partnership from the folks at Scout.com brings a big benefit to new Tiger Blitz/Scout members!by Billy EmbodyTiger BlitzFriday at 11:18 AMLSU Coaching Hot BoardFollowing Les Miles departure, there's sure to be plenty of high-profile candidates wanting to coach the Tigers and Scout keeps you up to date with the latest on LSU football's…by Billy EmbodyTiger Blitz10/14/2016Lane Kiffin's offense drastically differentLSU coach Ed Orgeron addressed the media after practice on Tuesday and then touched on similar things like Lane Kiffin's offense, LSU's offensive line and preparing LSU's offense…by Billy EmbodyTiger BlitzYesterday at 8:12 AMDid LSU release a Heisman hype video?After LSU running back Leonard Fournette's record-setting performance against Ole Miss, did LSU release a Heisman hype video for him? You be the judge.by Billy EmbodyTiger BlitzTuesday at 8:54 AMUpdated Scout 300 Trend Meter For 2017The updated Scout 300 Trend Meter for the 2017 class takes a look at which schools are trending for each uncommitted member in the Scout 300.by StaffScout FootballTuesday at 5:40 AM5-Star 'Bama Pledge Visits Another SEC SchoolJACKSONVILLE, Fla.-- Dylan Moses shocked everyone when he decided to pledge to Alabama earlier this month. The five-star linebacker was not planning to take anymore visits, however…by Jacquie FranciulliScout FootballMonday at 10:26 AMSEC Player of the Week: Leonard FournetteLSU RB Leonard Fournette is once again Scout's SEC Player of the Weekby StaffScout CFBMonday at 8:51 AMLSU turning finesse into power in run gameWhile LSU could have reinvented the wheel when Steve Ensminger took over the Tigers' offense, LSU instead turned finesse into power in their running game.by Murf BaldwinTiger BlitzMonday at 4:00 AMLoad More

value: This website is an integrated sports publishing company that produces Internet content covering hundreds of professional and college teams across America. The company was founded in 2001 and was acquired by Fox Sports in 2005.


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In addition to the original index-tracking E.T.F.’s, there are now products that track commodities — like gold, silver and oil — as well as currencies, sectors and specific industries. Some E.T.F.’s even bet on the direction of the market. A word to the wise: You might as well go to Vegas and bet it all on black.
Some E.T.F.’s have abandoned the index-based model altogether and track stocks chosen by sophisticated computer models. Actively managed E.T.F.’s are also beginning to hit the market, but their strategies are largely unproven. Proponents have said that actively managed E.T.F.’s will be less costly and more tax-efficient than active mutual funds, but it is far too early to tell how they will ultimately fare.When choosing an E.T.F., stick with well-known and time-tested providers such as Barclays Global Investors, Vanguard, and State Street. In most cases, you want to invest in the least expensive funds with recognizable indexes. It’s also wise to invest in larger funds with more assets, which means they will be easier and less expensive to trade. Investing in any fund with less than $50 million in assets is not advisable.Finally, remember the reason investors were attracted to the original batch of E.T.F.’s., and do not stray too far afield. Continue reading the main story

value: This website is an American daily newspaper based in New York City with a worldwide readership. Founded in 1851, the Times has since won 132 Pulitzer Prizes, the most of any newspaper, and has long been regarded within the industry as a national "newspaper of record".


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H. SMITH Obituary - Manassas, Virginia | Legacy.com
HomeObituariesH. SELWYN SMITH Obituary
Get email updates about H. SMITH delivered directly to your inbox.
I worked with this wonderful man from 1986 until he retired. I looked to him for advice a few times for something going on in my life and he was always kind enough to talk to me and give me his opinions. He was like a 2nd father to me in that respect. May his family find assurance in knowing that he is in heaven right now where each and everyone of you will join up with him one day. Rest in Peace "JUDGE".
August 26, 2013 | Calverton, VA

value: This website is a United States-based website founded in 1998, the world's largest commercial provider of online memorials. The Web site hosts obituaries and memorials for more than 70 percent of all U.S. deaths.


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THE Kitchen GOD'S Wife BY AMY TAN 2006 Paperback 0143038109 | eBay
|Listed as The Kitchen God's Wife by Amy Tan (2006, Paperback) in category: Books
The Kitchen God's Wife - Tan, Amy ...
The Kitchen God's Wife 0143038109
NEW The Kitchen God's Wife by Amy T...
THE KITCHEN GOD'S WIFE - AMY TAN...
Classics, Vol. 15 by Styx (CD, Oct-1987,...
Dazed and Confused by Various Artists (C...
Dinsney Children's Sing-Along Book,...
Hand Painted Collector Plate,...
Legacy Mobile Monitor 5.5" Color LCD
The Wizard of Oz by Weekly Reader Boo...
Little Black Dress with baloon hem, crinkle...
Vintage Electronic Sphygmomanometer...
The Premiere Collection Encore,...
Details about The Kitchen God's Wife by Amy Tan 2006 paperback
The Kitchen God's Wife by Amy Tan 2006 paperback
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eBay item number:191251259596
... Read moreabout the condition	ISBN-10: 0143038109
SynopsisWinnie and Helen have kept each other's worst secrets for more than fifty years. Now, because she believes she is dying, Helen wants to expose everything. And Winnie angrily determines that she must be the one to tell her daughter, Pearl, about the past--including the terible truth even Helen does not know. And so begins Winnie's story of her life on a small island outside Shanghai in the 1920s, and other places in China during World War II, and traces the happy and desperate events tha led to Winnie's coming to America in 1949.Product IdentifiersISBN-100143038109ISBN-139780143038108Key DetailsAuthorAmy TanNumber Of Pages416 pagesFormatPaperbackPublication Date2006-09-21LanguageEnglishPublisherPenguin Group (USA) IncorporatedDimensionsWeight12.2 OzHeight0.9 In.Width5.4 In.Length8 In.Target AudienceGroupTradeGrade FromTwelfth GradeGrade ToupAge Range18 - UPClassification MethodDewey Decimal813/.54Dewey Edition20Reviews"A beautiful book...what fascinates in The Kitchen God's Wifeis not only the insistent storytelling but the details of Chinese life and tradition; not only how people lived but how their sensibility shines through, most notably in their speech. Amy Tan has a command with language in which event and concrete perception jump into palpable metaphor, and images from the daily world act like spiritual agents." - Los Angeles Times "Remarkable...mesmerizing...compelling.... An entire world unfolds in Tolstoyan tide of event and detail....Give yourself over to the world Ms. Tan creates for you." - The New York Times Book Review	Certain data records © 2014 Bowker. Rights in cover images reserved by owners.
Item location: Decatur, Alabama, United States	Shipping to: Worldwide
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13021714225
13056224600
12995388235
13024820225
13056337296
Starting at $28.65	The Evolution of Civilizations: An Introduction to Historical Analysis
Starting at $2.04	The Anglo-American establishment : from Rhodes to Cliveden
Starting at $4.00	The Creature from Jekyll Island: A Second Look at the Federal Reserve
Starting at $12.98	The Evolution of Civilizations: An Introduction to Historical Analysis
Starting at $2.00	Tragedy and hope : a history of the world in our time.


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Mini USB 19Keys Numeric 3 Light Color Keyboard Backlit Keypad Wired For Notebook | eBay
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Mini USB 19Keys Numeric 3 Light Color Keyboard Backlit Keypad Wired For Notebook
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Implantable Cardiac Alert System for Early Recognition of ST-Segment Elevation Myocardial Infarction. - PubMed - NCBI
1 selected item: 30842028
Gibson CM1, Holmes D2, Mikdadi G3, Presser D4, Wohns D5, Yee MK6, Kaplan A7, Ciuffo A8, Eberly AL 3rd9, Iteld B10, Krucoff MW11.
Division of Cardiovascular Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts. Electronic address: mgibson@bidmc.harvard.org.
Innovative Medical Research LLC, Covington, Louisiana.
Division of Cardiovascular Medicine, Spectrum Health, Frederick Meijer Heart and Vascular Institute, Grand Rapids, Michigan.
Banner Heart Hospital, Mesa, Arizona.
Sentara Healthcare Norfolk, Norfolk, Virginia.
Division of Cardiology, Department of Internal Medicine, Greenville Health System, Greenville, South Carolina.
Louisiana Heart Center, Hammond, Louisiana.
Symptoms remain a poor prompt for acute coronary syndromes (ACS). Timely restoration of perfusion in ST-segment elevation myocardial infarction is associated with improved left ventricular function and survival.
This report details the results of ALERTS (AngelMed for Early Recognition and Treatment of STEMI), a multicenter, randomized trial of an implantable cardiac monitor that alerts patients with rapidly progressive ST-segment deviation.
High-risk ACS subjects (N = 907) were randomized to a control (alarms deactivated) or treatment group for 6 months, after which alarms were activated in all subjects. The primary safety endpoint was absence of system-related complications (>90%). The composite primary efficacy endpoint was cardiac/unexplained death, new Q-wave myocardial infarction, or detection to presentation time >2 h.
Safety was met with 96.7% freedom from system-related complications (n = 30). The efficacy endpoint for a confirmed occlusive event within 7 days was not significantly reduced in the treatment compared with control group (16 of 423 [3.8%] vs. 21 of 428 [4.9%], posterior probability = 0.786). Within a 90-day window, alarms significantly decreased detection to arrival time at a medical facility (51 min vs. 30.6 h; Pr [pt < pc] >0.999). In an expanded analysis using data after the randomized period, positive predictive value was higher (25.8% vs. 18.2%) and false positive rate significantly lower in the ALARMS ON group (0.164 vs. 0.678 false positives per patient-year; p < 0.001).
The implantable cardiac system detects early ST-segment deviation and alerts patients of a potential occlusive event. Although the trial did not meet its pre-specified primary efficacy endpoint, results suggest that the device may be beneficial among high-risk subjects in potentially identifying asymptomatic events. (AngelMed for Early Recognition and Treatment of STEMI [ALERTS]; NCT00781118).
ST-segment elevation myocardial infarction; acute coronary syndrome; implantable monitoring device; symptom-to-door time
ClinicalTrials.gov/NCT00781118

value: This website is part of the United States National Library of Medicine , a branch of the National Institutes of Health . It is approved and funded by the government of the United States.


text:
100% Reflective Class Diagram Creation Tool - CodeProject 10,885,501 members (116,814 online)
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Nabokov earns 50th career shutout helping Sharks climb to top in West
Sharks clinch playoff berth with win over StarsDallas Stars San Jose Sharks2283dAuston Matthews ready to make his markToronto Maple Leafs7hScott BurnsideBiggest early moves of the draftCalgary Flames5hScott BurnsideLeafs take Matthews No. 1; Laine to Jets at No. 2Toronto Maple Leafs4hFlames acquire goaltender Elliott from BluesSt. Louis Blues6hYzerman not giving up on re-signing StamkosTampa Bay Lightning5hHabs deal Eller to Caps, get Blackhawks' ShawMontreal Canadiens6hPierre LeBrunWings unload Datsyuk cap hit in Arizona tradeDetroit Red Wings4hPierre LeBrunNHL Trade Tracker4dMatthews goes first, but trades define draft night5hESPN Stats & InformationReport: Police again investigating Sabres' KaneBuffalo Sabres5hFlames, Oilers looking for key additions20hKlima twins: Like father, like sons?Detroit Red Wings2dTal PinchevskyFinding rare gold at the annual awards gala2dScott BurnsidePhotos: NHL awards from Las Vegas2dPlayer agency trying something different2dTal PinchevskyVegas counting on NHL to broaden Sin City identity2dArash MarkaziHow the NHL can make it work in Las Vegas2dOfficial rules around expansion, expansion draft format2dMeet the most interesting man in hockey3dScott BurnsideThis is what we'd call the Las Vegas team10dNHL logos redesigned with Vegas flair15dDrive-by interview with a Stanley Cup-carrying Sidney Crosby2dScott BurnsideRumblings: Business as usual for draft host Buffalo3dPierre LeBrunOur Las Vegas expansion franchise roster3dTal PinchevskyHighlights of the 2016-17 NHL scheduleBuffalo Sabres3dJoe McDonaldPoile has words of wisdom for Vegas GMFlorida Panthers4dScott BurnsideVegas vote, Stamkos' status among top 5 NHL storylines8dScott BurnsideStars re-sign Jordie Benn to avoid free agencyDallas Stars15hWild buy out final year of Vanek's contractMinnesota Wild12hPredators part-owner suing team, chairmanNashville Predators1dNabokov earns 50th career shutout helping Sharks climb to top in West
FacebookTwitterPinterestEmailcommentMar 26, 2010Associated Press FacebookTwitterPinterestEmailprintcommentSAN JOSE, Calif. -- Although Evgeni Nabokov gets visibly uncomfortable in the spotlight, the San Jose goalie allowed himself to enjoy it for a moment after his 50th career shutout.Nabokov just hopes his fellow Sharks will keep taking the attention away from him over the next few weeks as they build toward a sixth straight playoff run.Nabokov made 27 saves for his third shutout of the season, and the Sharks clinched a playoff berth while climbing back atop the Western Conference standings with a 3-0 victory over the Dallas Stars on Thursday night."I wanted to get this one out of the [way], but I think it's more important for the way we played," Nabokov said with a shrug. "Maybe the shutout will help, I don't know."Defensemen Dan Boyle and Rob Blake scored power-play goals in the second period for San Jose, which has won two straight after an 0-5-1 skid over the previous 10 days. Nabokov took each of the Sharks' five regulation losses during the skid, giving up 19 goals and worrying fans about his playoff readiness."It's a building process, getting ready for the playoffs," Nabokov said. "I think that's more important, and that's what we wanted to do. ... We can play this way, where we don't have many turnovers and we don't give up too many odd-man rushes, if any."Just as he's done in previous seasons, Nabokov is pulling himself out of a late-season funk while lifting his teammates. He has 39 victories to tie Los Angeles' Jonathan Quick and Phoenix's Ilya Bryzgalov for the second-most in the league behind New Jersey's Martin Brodeur (40).This shutout wasn't the toughest of his career, but it included a handful of stunning saves, including a sprawling breakaway stop in the final minute.With sturdy defense and 41 shots on Dallas goalie Marty Turco, the Sharks formally clinched their another postseason trip while earning their 100th point in the first of three games at the Shark Tank in four days. San Jose, which has four straight 100-point seasons, also jumped one point ahead of Chicago, which has a game in hand on the Sharks."As much as it's about us coming out of the forest, it's great to see Nabby only giving up one goal in the last two games," Sharks coach Todd McLellan said.Logan Couture scored his second NHL goal in the third period for the Sharks, whose power play had managed just one goal during the just-completed six-game trip before connecting twice against Dallas, which has one of the NHL's worst penalty-killing units."Yeah, [penalty-killing] is a concern," Dallas coach Marc Crawford said. "We couldn't win a draw tonight, which was significant. At least we had lots of opportunities to work on it tonight. ... The players have to get a feel for what is being called, and that was very tough tonight, very tough."Turco stopped 38 shots for the 10th-place Stars, who are nine points behind Detroit for the West's final playoff spot with eight games to play -- and the Red Wings have a game in hand. Dallas' near-.500 play over the past two weeks simply hasn't been enough to keep up with the surging Wings."Marty played well," Stars defenseman Nicklas Grossman said. "He made a lot of saves for us. We have to help him out a little bit. ... We had a few penalties called against us and they took advantage and we had to start chasing the game. Their goalie played extremely well."San Jose's skid started with an overtime home loss to lowly Florida and went on to include losses to four more teams likely to miss the playoffs: Anaheim, Dallas, Calgary and Edmonton. The slump ended with a win at Minnesota earlier this week, and McLellan stuck with the same reconfigured lines from that game."Obviously, we learned something there," McLellan said of Minnesota. "We used that same recipe again tonight, and now it's [about] how long can we hold on to that recipe and continue playing this way, because it is the right way to play."Dallas annually is among the Sharks' toughest opponents, winning nine of its previous 12 games in San Jose -- but without Mike Modano, who had an appendectomy 10 days ago, the Stars weren't as intimidating.After Boyle scored his 400th point early in the second period, Blake added another power-play goal less than 3 minutes later when a rebound of Patrick Marleau's shot ricocheted right to him in the slot. The San Jose captain's goal was his first in 16 games since Feb. 6 -- and the 135th power-play goal of his career, tying him with Paul Coffey for the career NHL lead.Game notes

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au sunglass holder | eBay
Skip to main contenteBayShop by categoryShop by categoryEnter your search keywordAll CategoriesAlcohol & FoodAntiquesArtBabyBooks, MagazinesBusinessCamerasCars, Bikes, BoatsClothing, Shoes, AccessoriesCoinsCollectablesComputers/Tablets & NetworkingCraftsDolls, BearsElectronicsGift CardsHealth & BeautyHome & GardenHome AppliancesHome EntertainmentIndustrialJewellery & WatchesMoviesMusicMusical InstrumentsPet SuppliesPhones & AccessoriesPottery, GlassServicesSporting GoodsStampsTickets, TravelToys, HobbiesVehicle Parts & AccessoriesVideo Games & ConsolesLots More...AdvancedG'day (Sign in to bid or buy) eBay Deals Sell Help & Contact My eBayExpand My eBay My eBay Summary Bids/Offers Watch List Wish List All Lists Purchase History Selling/sold My Collections Followed searches My messagesNotification {"url":"http://ir.ebaystatic.com/cr/v/c1/thirtysevens.jpg","maxViews":4,"imgSize":37,"expiry":300000,"timeout":250} Related: xa 1 18 ford au sunglass holder
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Women Men Stud Earrings 925 Sterling Silver Round Cut White Purple CZ Gift Box | eBay
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Daya I. Perkins - Semantic Scholar
Daya I. Perkins
James R. Trudell28
Daryl L. Davies28
S. John Mihic26
Ronald L. Alkana26
Neil L. Harrison25
61James R. Trudell
54Daryl L. Davies
52Ronald L. Alkana
46Liana Asatryan
42Gregg E. Homanics
Liana Asatryan, Maya Popova, Daya I. Perkins, James R. Trudell, Ronald L. Alkana, Daryl L Davies
Roles for loop 2 residues of alpha1 glycine receptors in agonist activation.
Daniel K. Crawford, Daya I. Perkins, James R. Trudell, Edward J. Bertaccini, Daryl L Davies, Ronald L. Alkana
The present study tested the hypothesis that several residues in Loop 2 of alpha1 glycine receptors (GlyRs) play important roles in mediating the transduction of agonist activation to channel gating.… (More)
Daya I. Perkins, James R. Trudell, Daniel K. Crawford, Ronald L. Alkana, Daryl L Davies
The present studies used increased atmospheric pressure in place of a traditional pharmacological antagonist to probe the molecular sites and mechanisms of ethanol action in glycine receptors… (More)
Loop 2 structure in glycine and GABA(A) receptors plays a key role in determining ethanol sensitivity.
Daya I. Perkins, James R. Trudell, Daniel K. Crawford, Liana Asatryan, Ronald L. Alkana, Daryl L Davies
The present study tests the hypothesis that the structure of extracellular domain Loop 2 can markedly affect ethanol sensitivity in glycine receptors (GlyRs) and gamma-aminobutyric acid type A… (More)
Glycine receptors (GlyRs) are recognized as the primary mediators of neuronal inhibition in the spinal cord, brain stem and higher brain regions known to be sensitive to ethanol. Building evidence… (More)
Anna Naito, Karan H. Muchhala, +5 authors Ronald L. Alkana
A critical obstacle to developing effective medications to prevent and/or treat alcohol use disorders is the lack of specific knowledge regarding the plethora of molecular targets and mechanisms… (More)
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A decamer duplication in the 3' region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred. - PubMed - NCBI
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See comment in PubMed Commons belowProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-5.A decamer duplication in the 3' region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred.Vidal R1, Revesz T, Rostagno A, Kim E, Holton JL, Bek T, Bojsen-Møller M, Braendgaard H, Plant G, Ghiso J, Frangione B.Author information1Department of Pathology, New York University School of Medicine, New York 10016, USA. vidalr01@popmail.med.nyu.eduAbstractFamilial Danish dementia (FDD), also known as heredopathia ophthalmo-oto-encephalica, is an autosomal dominant disorder characterized by cataracts, deafness, progressive ataxia, and dementia. Neuropathological findings include severe widespread cerebral amyloid angiopathy, hippocampal plaques, and neurofibrillary tangles, similar to Alzheimer's disease. N-terminal sequence analysis of isolated leptomeningeal amyloid fibrils revealed homology to ABri, the peptide originated by a point mutation at the stop codon of gene BRI in familial British dementia. Molecular genetic analysis of the BRI gene in the Danish kindred showed a different defect, namely the presence of a 10-nt duplication (795-796insTTTAATTTGT) between codons 265 and 266, one codon before the normal stop codon 267. The decamer duplication mutation produces a frame-shift in the BRI sequence generating a larger-than-normal precursor protein, of which the amyloid subunit (designated ADan) comprises the last 34 C-terminal amino acids. This de novo-created amyloidogenic peptide, associated with a genetic defect in the Danish kindred, stresses the importance of amyloid formation as a causative factor in neurodegeneration and dementia.PMID: 10781099 PMCID: PMC18333 DOI: 10.1073/pnas.080076097 [Indexed for MEDLINE] Free PMC ArticleShareImages from this publication.See all images (7)Free textFigure 1Isolation of ADan. Tris⋅Tricine SDS/PAGE (16%) of the amyloid protein isolated from leptomeningeal vessels stained with Coomassie blue. The 4-kDa band (line 2, arrowhead) was subjected to Edman N-terminal amino acid sequence analysis after being transferred onto an Immobilon-P membrane. Line 1: Low molecular-mass markers (Amersham) in kDa.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 2Analysis of PCR-amplified genomic DNA in the Danish kindred. (A) Polyacrylamide gel showing the restriction enzyme analysis performed as described on ref. . Genomic DNA was amplified with oligonucleotides F and R and analyzed with XbaI from a normal control (lane 1), a patient with FDD (lane 2), and a patient with FBD (lane 3). The arrowhead indicates undigested DNA (191 bp), and the arrows indicate digested fragments of 116 and 75 bp. (B) Metaphor agarose gel (4%) of PCR-amplified fragments by using primers F and R2. Lane 1: 123 molecular-mass marker. Lane 2: PCR product of amplification of the BRI gene in a patient with FDD. Lane 3: PCR amplification of a normal control. Amplification in the same conditions by using genomic DNA from patients with FBD gave identical results as seen in normal controls. The extra band observed in the Danish kindred (151 bp) is indicated by an arrow.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 3Nucleotide and amino acid sequence of ADan. Nucleotide and translated amino acid sequence of the wild-type BRI gene between nucleotides 730 to 824 and the corresponding sequence in amyloid ADan. A gap in the wild-type BRI sequence was added to show the insertion of the 10 nucleotides that are duplicated (underlined) in FDD patients. Nucleotides are numbered in the 5′ to 3′ orientation. Termination codons are noted in bold. Amino acid sequences are in one-letter code.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 4MS and Western blot analysis of leptomeningeal ADan. (A) MS analysis of the leptomeningeal amyloid isolated from the Danish case. * indicates formylated species (+28 units of mass), and @ indicates species with pyroglutamate at the amino terminus (−18 units of mass). The sequence of the full-length amyloid (34 amino acids) is indicated in one-letter code. (B) Western blotting analysis of purified amyloid fibrils from leptomeningeal vessels of cases with FDD (lanes 1 and 4) and FBD (lanes 2 and 3) reacted against anti-ADan antibody (5282) (lanes 1 and 2) and anti-ABri antibody (338) (lanes 3 and 4). Molecular masses are in kDa.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 5Immunohistochemical analysis. Parenchymal vessels of temporal sections (A) immunostained with Ab 5282 (×40) and (B) observed under polarized light after Congo red staining (×40). Examples of amyloid angiopathy with perivascular deposits in the subiculum (C) and granular cell layer of the cerebellar cortex (D) (Ab 5282; ×200). All regions of the hippocampus contain numerous Ab 5282-positive plaques (E) giving a good outline of the anatomy of the hippocampus (Ab 5282; ×4). Cotton wool-like plaques are present in the CA4 subregion of the hippocampus (F), whereas confluent looser deposits are characteristic in other subregions including CA1 (G) (Ab 5282; ×125). Cerebellum showed positivity in leptomeninges, leptomeningeal, and parenchymal blood vessels (H), with a perivascular plaque around the blood vessel in the granular cell layer (Ab 5282; ×30). Paraffin sections, hematoxylin counter stain.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 6Analysis of the 10-nt duplication in the BRI precursor protein gene in the Danish kindred. Members of the Danish family pedigree are shown in A and B. Roman numbers indicate different generations. Only affected members (filled symbols) are indicated. Genomic DNA was isolated from blood samples of cases AII1, AII2, and AIII1, and from formalin tissue of samples BII2 and BIII1 and from brain tissue of case IV1. In all cases, the 10-nt duplication was determined after cloning and sequencing of the amplified DNA.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Figure 7Schematic representation of the amyloid precursor proteins and amyloid peptides in patients affected with FBD and FDD. The BRI gene codifies a putative single transmembrane-type II precursor protein of 266 amino acids. Different genetic defects in the BRI gene, namely a point mutation in FBD (Stop-267→Arg) and a 10-nt duplication (795–796ins TTTAATTTGT) in FDD, originate the two precursor molecules ABriPP and ADanPP, respectively. Although originated by different genetic defects in the BRI gene, both mutated precursors have 277 amino acids. The scissors indicate the cleavage site of the precursor molecules between codons 243 (Arg) and 244 (Glu) that releases the ABri and ADan peptides. Both amyloids have identical N-terminal amino acid sequence (first 22 amino acids) and a completely different C-terminal sequence (last 12 amino acids). The diamond indicates the single glycosylation site at position 170.A decamer duplication in the 3′ region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindredProc Natl Acad Sci U S A. 2000 Apr 25;97(9):4920-4925.Publication types, MeSH terms, Substances, Secondary source ID, Grant supportPublication typesResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.MeSH terms3' Untranslated Regions/genetics*Amino Acid SequenceAmyloid/analysisAmyloid/genetics*Base SequenceDementia/genetics*Dementia/pathologyDenmarkEuropean Continental Ancestry GroupFemaleGene DuplicationHumansMaleMembrane GlycoproteinsMembrane ProteinsMolecular Sequence DataPedigreeSequence AlignmentSequence Homology, Amino AcidTemporal Lobe/blood supply*Temporal Lobe/pathologySubstances3' Untranslated RegionsAmyloidITM2B protein, humanMembrane GlycoproteinsMembrane ProteinsSecondary source IDGENBANK/AF246221Grant supportAG10953/AG/NIA NIH HHS/United StatesR01 AG008721/AG/NIA NIH HHS/United StatesAG05891/AG/NIA NIH HHS/United StatesAG08721/AG/NIA NIH HHS/United StatesR37 AG005891/AG/NIA NIH HHS/United StatesLinkOut - more resourcesFull Text SourcesHighWireEurope PubMed CentralPubMed CentralOther Literature SourcesCOS Scholar UniverseCited by Patents in - The LensMedicalDementia - MedlinePlus Health InformationMiscellaneousOMIM: Data: Gene Annotation - SciCrunchClinical Genomic Database: Data: Gene Annotation - SciCrunchPubMed Commons home