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Patent US6123710 - Process and article for knee reconstruction - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsThe invention comprises a method of reconstructing the anterior cruciate ligament in the right knee of a patient comprising the steps of forming a tendon graft of tendon, other soft tissue, ligament (including bone blocks if required), or artificial tendon; forming a hole into or through the lateral...http://www.google.com/patents/US6123710?utm_source=gb-gplus-sharePatent US6123710 - Process and article for knee reconstructionAdvanced Patent SearchPublication numberUS6123710 APublication typeGrantApplication numberUS 08/630,352Publication dateSep 26, 2000Filing dateApr 11, 1996Priority dateApr 12, 1995Fee statusPaidPublication number08630352, 630352, US 6123710 A, US 6123710A, US-A-6123710, US6123710 A, US6123710AInventorsLeo A. Pinczewski, Gregory J. RogerOriginal AssigneeSmith & Nephew, Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (56), Non-Patent Citations (30), Referenced by (92), Classifications (14), Legal Events (5) External Links: USPTO, USPTO Assignment, EspacenetProcess and article for knee reconstruction
US 6123710 AAbstract
Once the tendon graft 21 has been harvested suitable holes 22 and 23 are drilled in the femur 24 and the tibia 25 to receive the tendon graft 2 in known manner. The femoral hole 22 is drilled from a point 30� from the top of the notch (the 11 o'clock position) and 5 mm from the back of the intercondylar notch 26 on a lateral wall thereof. A guide hole is drilled approximately 20� anteriorly and 30� laterally with respect to the femur. A beath pin is then placed through the hole and a cannulated awl or drill is used to enlarge the hole 22 to a size suitable to receive the tendon graft 21.
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No. 72, Jan./Feb. 1983, pp. 85 89, Vascularized Patella Tendon Graft with Rigid internal Fixation for Anterior Cruciate Ligament Insufficiency.30Lambert K.L., Clinical Orthopedics and Related Research, No. 72, Jan./Feb. 1983, pp. 85-89, "Vascularized Patella Tendon Graft with Rigid internal Fixation for Anterior Cruciate Ligament Insufficiency."* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS6283973 *Oct 27, 1999Sep 4, 2001Depuy Orthopaedics, Inc.Strength fixation deviceUS6752830Jul 19, 2000Jun 22, 2004Ethicon, Inc.Apparatus and method for reconstructing a ligamentUS6808528 *Feb 22, 2001Oct 26, 2004Ethicon, Inc.Apparatus and method for securing a graft ligament in a bone tunnelUS7022124 *Apr 11, 2002Apr 4, 2006Pentax CorporationSurgical instrumentUS7025786 *Jun 22, 2004Apr 11, 2006Ethicon, Inc.Apparatus and method for reconstructing a ligamentUS7494490Oct 13, 2004Feb 24, 2009Depuy Mitek, Inc.Apparatus and method for reconstructing a ligamentUS7594929Nov 21, 2003Sep 29, 2009Michel ColletteAnchoring screw for a relay strip or sutureUS7658751Sep 29, 2006Feb 9, 2010Biomet Sports Medicine, LlcMethod for implanting soft tissueUS7749250Jul 6, 2010Biomet Sports Medicine, LlcSoft tissue repair assembly and associated methodUS7857830Oct 9, 2007Dec 28, 2010Biomet Sports Medicine, LlcSoft tissue repair and conduit deviceUS7905903Nov 6, 2007Mar 15, 2011Biomet Sports Medicine, LlcMethod for tissue fixationUS7905904Jan 15, 2008Mar 15, 2011Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS7909851Mar 22, 2011Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS7914539Dec 5, 2005Mar 29, 2011Biomet Sports Medicine, LlcTissue fixation deviceUS7959650Aug 22, 2008Jun 14, 2011Biomet Sports Medicine, LlcAdjustable knotless loopsUS8034090Mar 21, 2006Oct 11, 2011Biomet Sports Medicine, LlcTissue fixation deviceUS8070750Dec 6, 2011Depuy Mitek, Inc.Tunnel notcher and guidewire delivery deviceUS8088130May 29, 2009Jan 3, 2012Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to a boneUS8110006 *May 6, 2010Feb 7, 2012Inbone Technologies, Inc.Fibular stiffener and bony defect replacerUS8118836Aug 22, 2008Feb 21, 2012Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to a boneUS8128658Aug 22, 2008Mar 6, 2012Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to boneUS8137382Aug 22, 2008Mar 20, 2012Biomet Sports Medicine, LlcMethod and apparatus for coupling anatomical featuresUS8221454Oct 27, 2009Jul 17, 2012Biomet Sports Medicine, LlcApparatus for performing meniscus repairUS8231654May 6, 2011Jul 31, 2012Biomet Sports Medicine, LlcAdjustable knotless loopsUS8251998Feb 12, 2008Aug 28, 2012Biomet Sports Medicine, LlcChondral defect repairUS8273106Dec 22, 2010Sep 25, 2012Biomet Sports Medicine, LlcSoft tissue repair and conduit deviceUS8292921Mar 11, 2011Oct 23, 2012Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS8298262Jun 22, 2009Oct 30, 2012Biomet Sports Medicine, LlcMethod for tissue fixationUS8303604Sep 30, 2009Nov 6, 2012Biomet Sports Medicine, LlcSoft tissue repair device and methodUS8317825Apr 7, 2009Nov 27, 2012Biomet Sports Medicine, LlcSoft tissue conduit device and methodUS8337525Mar 11, 2011Dec 25, 2012Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS8343227May 27, 2010Jan 1, 2013Biomet Manufacturing Corp.Knee prosthesis assembly with ligament linkUS8361113Jun 22, 2009Jan 29, 2013Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to a boneUS8409253Apr 2, 2013Biomet Sports Medicine, LlcSoft tissue repair assembly and associated methodUS8500818May 27, 2010Aug 6, 2013Biomet Manufacturing, LlcKnee prosthesis assembly with ligament linkUS8506597Oct 25, 2011Aug 13, 2013Biomet Sports Medicine, LlcMethod and apparatus for interosseous membrane reconstructionUS8551140Jul 13, 2011Oct 8, 2013Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to boneUS8562645May 2, 2011Oct 22, 2013Biomet Sports Medicine, LlcMethod and apparatus for forming a self-locking adjustable loopUS8562647Oct 29, 2010Oct 22, 2013Biomet Sports Medicine, LlcMethod and apparatus for securing soft tissue to boneUS8574235May 19, 2011Nov 5, 2013Biomet Sports Medicine, LlcMethod for trochanteric reattachmentUS8597327Nov 3, 2010Dec 3, 2013Biomet Manufacturing, LlcMethod and apparatus for sternal closureUS8608777Oct 21, 2011Dec 17, 2013Biomet Sports MedicineMethod and apparatus for coupling soft tissue to a boneUS8617176Aug 24, 2011Dec 31, 2013Depuy Mitek, LlcCross pinning guide devices and methodsUS8632569Dec 20, 2012Jan 21, 2014Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS8636798 *Apr 11, 2006Jan 28, 2014Depuy Mitek, LlcApparatus and method for reconstructing a ligamentUS8652171May 2, 2011Feb 18, 2014Biomet Sports Medicine, LlcMethod and apparatus for soft tissue fixationUS8652172Jul 6, 2011Feb 18, 2014Biomet Sports Medicine, LlcFlexible anchors for tissue fixationUS8672968Feb 8, 2010Mar 18, 2014Biomet Sports Medicine, LlcMethod for implanting soft tissueUS8672969Oct 7, 2011Mar 18, 2014Biomet Sports Medicine, LlcFracture fixation deviceUS8721684Mar 5, 2012May 13, 2014Biomet Sports Medicine, LlcMethod and apparatus for coupling anatomical featuresUS8747439Jul 10, 2006Jun 10, 2014P Tech, LlcMethod of using ultrasonic vibration to secure body tissue with fastening elementUS8771316Mar 5, 2012Jul 8, 2014Biomet Sports Medicine, LlcMethod and apparatus for coupling anatomical featuresUS8771352May 17, 2011Jul 8, 2014Biomet Sports Medicine, LlcMethod and apparatus for tibial fixation of an ACL graftUS8777956Aug 16, 2012Jul 15, 2014Biomet Sports Medicine, LlcChondral defect repairUS8801783May 27, 2010Aug 12, 2014Biomet Sports Medicine, LlcProsthetic ligament system for knee jointUS8808329Apr 3, 2012Aug 19, 2014Bonutti Skeletal Innovations LlcApparatus and method for securing a portion of a bodyUS8814902Jul 31, 2006Aug 26, 2014Bonutti Skeletal Innovations LlcMethod of securing body tissueUS8840645Feb 17, 2012Sep 23, 2014Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to a boneUS8845687Sep 17, 2013Sep 30, 2014Bonutti Skeletal Innovations LlcAnchor for securing a sutureUS8845699Mar 6, 2012Sep 30, 2014Bonutti Skeletal Innovations LlcMethod of securing tissueUS8900314Dec 19, 2012Dec 2, 2014Biomet Manufacturing, LlcMethod of implanting a prosthetic knee joint assemblyUS8932331Mar 5, 2012Jan 13, 2015Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to boneUS8936621Nov 3, 2011Jan 20, 2015Biomet Sports Medicine, LlcMethod and apparatus for forming a self-locking adjustable loopUS8968364May 17, 2011Mar 3, 2015Biomet Sports Medicine, LlcMethod and apparatus for fixation of an ACL graftUS8979847Jun 6, 2011Mar 17, 2015Biomet Manufacturing, LlcMethod and apparatus for implanting a knee prosthesisUS8998949Aug 16, 2006Apr 7, 2015Biomet Sports Medicine, LlcSoft tissue conduit deviceUS9005287Nov 4, 2013Apr 14, 2015Biomet Sports Medicine, LlcMethod for bone reattachmentUS9017381Apr 10, 2007Apr 28, 2015Biomet Sports Medicine, LlcAdjustable knotless loopsUS9078644Mar 8, 2010Jul 14, 2015Biomet Sports Medicine, LlcFracture fixation deviceUS9119712Aug 23, 2013Sep 1, 2015Cook Medical Technologies LlcMedical devices, systems, and kits for the medialization of a vocal cordUS9149267Nov 10, 2011Oct 6, 2015Biomet Sports Medicine, LlcMethod and apparatus for coupling soft tissue to a boneUS9173651Oct 22, 2012Nov 3, 2015Biomet Sports Medicine, LlcSoft tissue repair device and associated methodsUS9216078May 8, 2013Dec 22, 2015Biomet Sports Medicine, LlcMethod and apparatus for tibial fixation of an ACL graftUS20020151903 *Apr 11, 2002Oct 17, 2002Asahi Kogaku Kogyo Kabushiki KaishaSurgical instrumentUS20030125749 *Dec 27, 2001Jul 3, 2003Ethicon, Inc.Cannulated screw and associated driver systemUS20040225358 *Jun 22, 2004Nov 11, 2004Goble E MarloweApparatus and method for reconstructing a ligamentUS20040225360 *Mar 30, 2004Nov 11, 2004Malone David G.Devices and methods for facilitating controlled bone growth or repairUS20050065527 *Oct 13, 2004Mar 24, 2005Justin Daniel F.Apparatus and method for reconstructing a ligamentUS20050203523 *Mar 5, 2004Sep 15, 2005Wenstrom Richard F.Jr.Tunnel notcher and guidewire delivery deviceUS20060106390 *Nov 18, 2004May 18, 2006Jensen David GComposite bone fastenersUS20060142769 *Nov 21, 2003Jun 29, 2006Michel ColletteAnchoring screw for a relay strip or sutureUS20060265063 *Apr 11, 2006Nov 23, 2006Goble E MApparatus and method for reconstructing a ligamentUS20090093880 *Dec 12, 2008Apr 9, 2009Depuy Mitek, Inc.Apparatus and methos for reconstructing a ligamentUS20090105840 *Oct 18, 2007Apr 23, 2009Inbone Technologies, Inc.Fibular stiffener and bony defect replacerUS20100211075 *Aug 19, 2010Biomet Sports Medicine, LlcFracture Fixation DeviceUS20100217402 *May 6, 2010Aug 26, 2010Inbone Technologies, Inc.Fibular stiffener and bony defect replacerUSRE42526 *Jun 12, 2003Jul 5, 2011Arthrex, Inc.Bicortical tibial fixation of ACL graftsEP1941841A1 *Mar 4, 2005Jul 9, 2008DePuy Mitek, Inc.Tunnel notcher and guidewire delivery deviceEP2286744A1 *Mar 4, 2005Feb 23, 2011DePuy Mitek, Inc.Tunnel notcher and guidewire delivery deviceEP2292162A1 *Mar 4, 2005Mar 9, 2011DePuy Mitek, Inc.Tunnel notcher and guidewire delivery deviceEP2700384A2 *Aug 23, 2013Feb 26, 2014Ridgley, PamelaMedical devices, systems, and kits for medialization of a vocal cordWO2004045465A1 *Nov 21, 2003Jun 3, 2004Michel ColletteAnchoring screw for a relay strip or suture* Cited by examinerClassifications U.S. Classification606/304, 606/321International ClassificationA61F2/08, A61B17/86Cooperative ClassificationA61B17/8605, A61F2002/0858, A61F2002/0841, A61B17/8625, A61B17/864, A61F2/0811, A61F2/0805European ClassificationA61F2/08F, A61B17/86B, A61B17/86ALegal EventsDateCodeEventDescriptionJul 22, 1996ASAssignmentOwner name: SMITH & NEPHEW ENDOSCOPY, INC., MASSACHUSETTSFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:PINCZEWSKI, LEO A.;ROGER, GREGORY J.;REEL/FRAME:008057/0414Effective date: 19960715Nov 28, 1997ASAssignmentOwner name: SMITH & NEPHEW, INC., MASSACHUSETTSFree format text: CHANGE OF NAME;ASSIGNOR:SMITH & NEPHEW ENDOSCOPY, INC.;REEL/FRAME:008831/0625Effective date: 19961126Feb 18, 2004FPAYFee paymentYear of fee payment: 4Feb 28, 2008FPAYFee paymentYear of fee payment: 8Feb 22, 2012FPAYFee paymentYear of fee payment: 12RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services

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- Bears mailbag: expectations for Knox ESPN.com:
Bears mailbag: expectations for Knox By Jeff Dickerson
Johnny Knox may be able to make an even bigger impact under Mike Martz.Can tell me why people care so much about these offseason practices? I mean, they don’t wear pads or hit, who cares if certain players fail to show up? I love the NFL, but enough is enough. Thank you for your comments. --Philly T., Racine, Wisc. Coaches definitely care if players fail to show up. Every person on the Bears roster knows Lovie Smith expects full participation during the offseason voluntary workouts, and those who skip the sessions do so at their own risk. While players don’t wear pads, they do work out, polish up on technique, and in the case of the offense, begin to learn a brand new system. This year, in particular, it’s critical for skill position players to show up every day ready to work, because Mike Martz arrives with a reputation of not tolerating mistakes on the practice field. Better to screw up now then during training camp, when the stakes are much higher. Desmond Clark missed one week of the program, but he returned after receiving some clarity about his role on the team. Outside of fellow tight end Brandon Manumaleuna, I haven’t heard of many other offensive players skipping workouts. On defense, Danieal Manning reported a few weeks ago, leaving Charles Tillman as the only member of the secondary MIA. Tillman is likely trying to preserve his body for the upcoming season, but I’d be surprised if he fails to show up for the mandatory mini-camp that kickoffs on May 21. You media people are so annoying. All I read about is how tough [Mike] Martz’s offense is to learn. It’s football, not rocket science! They’ll figure it out. Do something useful with your time and stop wasting ours. -- Michael, Chicago
Since I obviously don’t play in the NFL, I can only relay things heard from current and former players in regards to Martz’s offense. I’m told it’s high volume, with more formations and play calls than anybody is used to learning. People say it’s all thrown at you so fast, that even assistant coaches are sometimes slow to pick it up. If you want to act like the learning curve is no big deal, fine by me, but I think it’s a mistake to underestimate the complexities of this offense, especially compared to what the Bears ran the last five years under Ron Turner. Nobody is saying any of the current players on this roster are incapable of learning the new offense, but I think adding a veteran quarterback and receiver, familiar with the scheme, would be a wise and prudent move. What’s the risk? All it would take is signing two guys for the veteran league minimum. What can we expect from our top rookie last year: Johnny Knox. How much better can Knox be under Martz? -- Alexander Franklin, Champaign, Ill. Good question. If Knox finds his role in Martz's offense, I bet he'll turn into a much more dangerous receiver. I thought at times last season Knox displayed good hands and toughness, in addition to that blazing speed. But I'd like to see a little more consistency from Knox in his sophomore campaign , because 235 of his 527 receiving yards came in just three games (Games 1, 2 and 13). I realize certain people get sensitive when addressing the issue of younger receivers learning the offense, but remember, Knox is only one season removed from playing at a small college. Isn't it fair to wonder if completely digesting a second brand new offense in two years could be a lengthy process for any receiver, especially one of the younger variety? But Knox will figure it out, and when that happens, I'd like to see 12 to 15 of his receptions go for 20 plus yards in 2010. Knox caught 7 passes for 20 plus yards in 2009.
JD, you talked last week about Kevin Shaffer at left guard, how is that going? Is he the front-runner? – Nate H., San Francisco I think a few people -- Shaffer, Josh Beekman, Lance Louis, Johan Asiata -- are going to have a legitimate chance to win the left guard job, but every report about Shaffer this offseason has been positive. Not only is he an experienced veteran, Shaffer is a pretty big guy, which gives him a leg up on Beekman. Personally, I think Beekman is a solid, athletic player, who loves to mix it up on the field. That being said, the Bears made it a point to beef up the interior of the offensive line, and while Frank Omiyale failed to pan out at guard in 2009, it sounds like the overall philosophy remains unchanged. Shaffer is still working at guard during the voluntary workouts, and will be one guy to watch closely during the preseason games. Jeff, do you anticipate any surprises on the final 53 man roster? – Jake Hardy, Dallas It’s way too early to make any final roster predictions, but here’s a battle I’ll be watching closely in camp: Kellen Davis vs. Richard Angulo. Probably not the tight end competition you were expecting, so let me explain myself. There is a strong chance the Bears keep four tight ends this year, even though most teams tend to keep only three, and that final spot could come down to Davis or Angulo. Conventional wisdom suggests Davis has the edge because of draft status, but Angulo is a strong blocker, not to mention a hard worker, who previously played for Martz in St. Louis and Tice in Jacksonville. After displaying a questionable work ethic as a rookie, Davis picked up his game last offseason when the Bears signed Michael Gaines, who was ultimatley released October 17. My understanding is Angulo will be much stiffer competition for Davis, so let's see if the 2008 fifth-round choice is up to the challenge.

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Flying Fear: Hypnosis Downloads Audiobook | Craig Beck | Audible.com
SAMPLE PLAY AUDIO SAMPLE http%3A%2F%2Fsamples.audible.com%2Fbk%2Facx0%2F058103%2Fbk_acx0_058103_sample.mp3+flashcontent023MEV4XTQ099MAYDJXH0
Are you afraid of flying? All self-limiting beliefs, phobias, and bad habits are simply bad programs buried in the subconscious area of the mind. Willpower is entirely ineffective in dealing with these issues because you are prevented from accessing the source of the problem. Craig Beck is a master hypnotist of 20 years standing and is also a respected timeline therapist and NLP master practitioner. He understands what makes people tick and more importantly how to access and remove the erroneous programs that cause us problems in everyday life. Designed to quickly help you overcome fear and be able to fly without worry or distress. Unique speed hypnosis technique for rapid results Instill unshakable inner peace & security Calm your nerves before or during any flight Destroy all traces of the fear of flying Replace your self-doubt with a new constructive habit ©2016 www.CraigBeck.com (P)2016 www.CraigBeck.com

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Drexel Hill, Pennsylvania, (Delaware County) 19026
Monsignor Bonner High School is an all-male Augustinian Catholic High School in the Roman Catholic Archdiocese of Philadelphia. It is located in Drexel Hill, Pennsylvania, United States. Bonner was created in 1953 as Archbishop Prendergast High School for Boys. In 1955, the current building was constructed and subsequently entitled Monsignor Bonner High School. The previously occupied building became the all-female Archbishop Prendergast High School. In 2012, Bonner merged with the all-girls Archbishop Prendergast High School to form Monsignor Bonner and Archbishop Prendergast High School.
Dennis Christopher - Film and TV Actor[3]
Jamie Kennedy - Comedian, Actor, Writer
^ MSA-CSS. "MSA-Commission on Secondary Schools". Retrieved 2009-05-26. ^ See Augustinian web reference http://www.stritahs.com/augustinianschools.htm
Retrieved from "https://en.wikipedia.org/w/index.php?title=Monsignor_Bonner_High_School&oldid=731504242"	Categories: Boys' schools in the United StatesAugustinian schoolsRoman Catholic secondary schools in PennsylvaniaRoman Catholic Archdiocese of PhiladelphiaEducational institutions established in 1953Schools in Delaware County, PennsylvaniaRugby league stadiums in the United States1953 establishments in PennsylvaniaHidden categories: Coordinates on WikidataPages using infobox school with unsupported parameters	Navigation menu
Add links	This page was last modified on 25 July 2016, at 19:50.

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Gill Jepson Books New, Rare & Used Books - Alibris
Gill Jepson is a teacher and Adult Education trainer. Now, as an author, she also freelances in schools, providing literacy and educational workshops. She has had a number of articles published, as well a short school history. She has written two other children's novel, Out of Time: The Secret of the Swan (Matador, 2011) and Guy the Grumpy Gargoyle (Matador, 2012). See less
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West Valley City Face & Skin Care - Deals in West Valley City, UT | Groupon
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Categories Beauty & Spas (4)Face & Skin (4)Facials (1)Microdermabrasion (1)
Specialized estheticians swipe fine sand crystals across the face, removing the dead and damaged skin cells
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\n \n One, Three, or Six Microdermabrasion Treatments at Corrective Skin Care (Up to 58% Off)\n
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Specialized estheticians swipe fine sand crystals across the face, removing the dead and damaged skin cells
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\n \n South Salt Lake City\n \n \n • 6.8 mi\n \n \n
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n cui-c-udc-featured-fallback\n \n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#068ff003-e16d-48ff-bb30-d7834895009d\"\n data-bhd=\"{"cardUUID":"068ff003-e16d-48ff-bb30-d7834895009d","cardSubtype":"LOCAL","title":{"type":"title","content":"One, Three, or Six Microdermabrasion Treatments at Corrective <strong>Skin Care</strong> (Up to 58% Off)"},"slot_1":{"type":"location-and-distance","content":"South Salt Lake City(6.8 mi)"},"slot_2":{"type":"number-bought","content":20},"value":{"type":"price","content":"$100 $45"}}\"\n data-bhw=\"HeroDeal\"\n data-bh-viewport=\"respect\"\n >\n\n \n
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\n \n One, Three, or Six Microdermabrasion Treatments at Corrective Skin Care (Up to 58% Off)\n
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\n \n South Salt Lake City\n \n \n • 6.8 mi\n \n \n
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The cleansing brush uses invigorating rotary action and bristles to clean out pores, exfoliate the skin, and promote collagen production
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#26954644-1b13-41c2-b1c6-b1fbfdf0524a\"\n data-bhd=\"{"cardUUID":"26954644-1b13-41c2-b1c6-b1fbfdf0524a","cardSubtype":"LOCAL","title":{"type":"title","content":"$19.99 for a <strong>Skin</strong>, <strong>Face</strong>, and Body-Cleansing Brush at Spin for Perfect <strong>Skin</strong> ($100 Value)"},"slot_2":{"type":"number-bought","content":250},"value":{"type":"price","content":"$100 $19.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#99ec4068-7a5c-4cac-90c5-f46d729ef0fe\"\n data-bhd=\"{"cardUUID":"99ec4068-7a5c-4cac-90c5-f46d729ef0fe","cardSubtype":"LOCAL","title":{"type":"title","content":"6- or 12-Pack of European Gold <strong>Face</strong> Masks from My Beauty Secrets USA (Up to 80% Off)"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":2},"value":{"type":"price","content":"$149 $29.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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Face masks infuse skin with collagen and 24K gold powder to reduce wrinkles and signs of aging, leaving skin brighter and smoother
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#88b5b832-63e1-46c3-9652-3ae8b1758764\"\n data-bhd=\"{"cardUUID":"88b5b832-63e1-46c3-9652-3ae8b1758764","cardSubtype":"LOCAL","title":{"type":"title","content":"One or Two 1.7oz of Retinol 2.5% High Potency Anti-Aging Cream from My Beauty Secrets USA"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":50},"value":{"type":"price","content":"$129 $21.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n \n One or Two 1.7oz of Retinol 2.5% High Potency Anti-Aging Cream from My Beauty Secrets USA\n
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Anti-aging cream helps improve skin texture, smooths wrinkles with essential oils and extracts that are sourced from organic farms
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#88b5b832-63e1-46c3-9652-3ae8b1758764\"\n data-bhd=\"{"cardUUID":"88b5b832-63e1-46c3-9652-3ae8b1758764","cardSubtype":"LOCAL","title":{"type":"title","content":"One or Two 1.7oz of Retinol 2.5% High Potency Anti-Aging Cream from My Beauty Secrets USA"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":50},"value":{"type":"price","content":"$129 $21.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#17a6814b-ab87-4abe-814f-28352d248ac6\"\n data-bhd=\"{"cardUUID":"17a6814b-ab87-4abe-814f-28352d248ac6","cardSubtype":"LOCAL","title":{"type":"title","content":"Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available."},"slot_2":{"type":"number-bought","content":40},"value":{"type":"price","content":"$40 $20"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n \n Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available.\n
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Extensive line of beauty products inventory features deep cleansing body washes, moisturizing serums, purifying face masks, and cosmetics
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#17a6814b-ab87-4abe-814f-28352d248ac6\"\n data-bhd=\"{"cardUUID":"17a6814b-ab87-4abe-814f-28352d248ac6","cardSubtype":"LOCAL","title":{"type":"title","content":"Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available."},"slot_2":{"type":"number-bought","content":40},"value":{"type":"price","content":"$40 $20"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n \n $19.99 for a Skin, Face, and Body-Cleansing Brush at Spin for Perfect Skin ($100 Value)\n
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The cleansing brush uses invigorating rotary action and bristles to clean out pores, exfoliate the skin, and promote collagen production
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#26954644-1b13-41c2-b1c6-b1fbfdf0524a\"\n data-bhd=\"{"cardUUID":"26954644-1b13-41c2-b1c6-b1fbfdf0524a","cardSubtype":"LOCAL","title":{"type":"title","content":"$19.99 for a <strong>Skin</strong>, <strong>Face</strong>, and Body-Cleansing Brush at Spin for Perfect <strong>Skin</strong> ($100 Value)"},"slot_2":{"type":"number-bought","content":250},"value":{"type":"price","content":"$100 $19.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#99ec4068-7a5c-4cac-90c5-f46d729ef0fe\"\n data-bhd=\"{"cardUUID":"99ec4068-7a5c-4cac-90c5-f46d729ef0fe","cardSubtype":"LOCAL","title":{"type":"title","content":"6- or 12-Pack of European Gold <strong>Face</strong> Masks from My Beauty Secrets USA (Up to 80% Off)"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":2},"value":{"type":"price","content":"$149 $29.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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Face masks infuse skin with collagen and 24K gold powder to reduce wrinkles and signs of aging, leaving skin brighter and smoother
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#99ec4068-7a5c-4cac-90c5-f46d729ef0fe\"\n data-bhd=\"{"cardUUID":"99ec4068-7a5c-4cac-90c5-f46d729ef0fe","cardSubtype":"LOCAL","title":{"type":"title","content":"6- or 12-Pack of European Gold <strong>Face</strong> Masks from My Beauty Secrets USA (Up to 80% Off)"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":2},"value":{"type":"price","content":"$149 $29.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#88b5b832-63e1-46c3-9652-3ae8b1758764\"\n data-bhd=\"{"cardUUID":"88b5b832-63e1-46c3-9652-3ae8b1758764","cardSubtype":"LOCAL","title":{"type":"title","content":"One or Two 1.7oz of Retinol 2.5% High Potency Anti-Aging Cream from My Beauty Secrets USA"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":50},"value":{"type":"price","content":"$129 $21.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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Anti-aging cream helps improve skin texture, smooths wrinkles with essential oils and extracts that are sourced from organic farms
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#88b5b832-63e1-46c3-9652-3ae8b1758764\"\n data-bhd=\"{"cardUUID":"88b5b832-63e1-46c3-9652-3ae8b1758764","cardSubtype":"LOCAL","title":{"type":"title","content":"One or Two 1.7oz of Retinol 2.5% High Potency Anti-Aging Cream from My Beauty Secrets USA"},"slot_1":{"type":"location-and-distance","content":"Salt Lake City"},"slot_2":{"type":"number-bought","content":50},"value":{"type":"price","content":"$129 $21.99"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n <figure class=\"card-ui cui-c-udc cui-c-udc-featured-list\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#17a6814b-ab87-4abe-814f-28352d248ac6\"\n data-bhd=\"{"cardUUID":"17a6814b-ab87-4abe-814f-28352d248ac6","cardSubtype":"LOCAL","title":{"type":"title","content":"Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available."},"slot_2":{"type":"number-bought","content":40},"value":{"type":"price","content":"$40 $20"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n \n Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available.\n
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Extensive line of beauty products inventory features deep cleansing body washes, moisturizing serums, purifying face masks, and cosmetics
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\n\n<figure class=\"card-ui cui-c-udc c-bdr-gray-clr\n \n cui-c-udc-featured-list-fallback\n \n \n \n cui-udc-responsive\"\n data-pingdom-info=\"purchasable-deal\"\n data-bhc=\"card:deal#17a6814b-ab87-4abe-814f-28352d248ac6\"\n data-bhd=\"{"cardUUID":"17a6814b-ab87-4abe-814f-28352d248ac6","cardSubtype":"LOCAL","title":{"type":"title","content":"Makeup, Skincare, and Wellness Products at Your Health Your Beauty (Up to 51% Off). Two Options Available."},"slot_2":{"type":"number-bought","content":40},"value":{"type":"price","content":"$40 $20"}}\"\n \n data-bh-viewport=\"respect\"\n >\n\n \n
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\n","gridCardsHtml":false}},"c2":{"uuid":"e34de00e-f716-4448-abfb-5d911c3859c6","content":{"title":"Face & Skin Care in West Valley City, UT"}},"c12":{"uuid":"e868587f-34c9-4fcb-b665-51b188d9e273","content":{"category":"Face & Skin","locationName":"West Valley City","columnLayout":"two-up","categoryLinks":{"links":[{"anchorText":"Massage in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/massage","indexed":true},{"anchorText":"Tanning in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/tanning","indexed":true},{"anchorText":"Spa in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/spa","indexed":true},{"anchorText":"Massage in Orem","url":"https://www.groupon.com/local/orem-ut/massage","indexed":true},{"anchorText":"Nail Salons in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/nail-salons","indexed":true},{"anchorText":"Hair Removal in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/hair-removal","indexed":true},{"anchorText":"Salons in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/salons","indexed":true},{"anchorText":"Facial in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/facial","indexed":true},{"anchorText":"Massage in Provo","url":"https://www.groupon.com/local/provo/massage","indexed":true},{"anchorText":"Massage in Ogden","url":"https://www.groupon.com/local/ogden-ut/massage","indexed":true}],"listHeading":"Popular Searches","tag":"CrosslinksCategoryLink"},"geoLinks":{"links":[{"anchorText":"Restaurants in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/restaurants","indexed":true},{"anchorText":"Gyms in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/gyms","indexed":true},{"anchorText":"Auto Repair & Maintenance in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/auto-repair","indexed":true},{"anchorText":"Laser Hair Removal in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/laser-hair-removal","indexed":true},{"anchorText":"Things To Do in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/things-to-do","indexed":true},{"anchorText":"Pizza in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/pizza","indexed":true},{"anchorText":"Yoga in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/yoga","indexed":true},{"anchorText":"Dental Implants in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/dental-implants","indexed":true},{"anchorText":"Chiropractor in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/chiropractor","indexed":true},{"anchorText":"Coffee Shops in Salt Lake City","url":"https://www.groupon.com/local/salt-lake-city/coffee-shops","indexed":true}],"listHeading":"Searches Nearby","tag":"CrosslinksGeoLink"}}},"c8":{"uuid":"7ff8003e-e6d5-11e5-9730-9a79f06e9478","content":{"editorial":{"widgetTitle":"Groupon Guide","withSidebar":true,"relatedArticles":[{"guid":"db303abf-37cd-46dc-829a-581dd65534fc","imageUrl":"//img.grouponcdn.com/seocms/y2DdufjojHtuKpPkBEKLDbBqRWG/brunette-woman-with-clear-skin-IPL-facial_jpg-600x390/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"
Learn all about how IPL treatments broad-spectrum light energy works to combat age spots and other skin discolorations.
","url":"/articles/what-is-ipl-treatment","title":"How IPL Treatments Use Light to Fight Sunlight Damage"},{"guid":"f411d2c3-b27f-4470-a22b-b0e87d8a9a6b","imageUrl":"//img.grouponcdn.com/seocms/mCFWPrQwBrDgKRmcHKU6cA/four-things-about-mud-baths-600x390/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"Prepare to sink into the wet and dirty world of mud treatments.","url":"/articles/why-taking-a-mud-bath-might-be-good-for-you","title":"Why Taking a Mud Bath Might Be Good for You"},{"guid":"1e4b87ec-edc1-4961-898c-4dbebc971f4d","imageUrl":"//img.grouponcdn.com/seocms/beNah42H9pdfBp7rHfGkD6/lipbalms_table_png-530x288/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"
We sought lip-balm advice from a dermatologist, then had our beauty team test four different types. The balm at the top of the list? It’s a tie.
","url":"/articles/what-is-the-best-lip-balm","title":"4 Lip Balms, 16 Days, 10 Sets of Lips: The Panic to Find the Best Lip Balm Before Winter Hits"},{"guid":"2696d453-7440-4566-98b1-0dad83f56215","imageUrl":"//img.grouponcdn.com/seocms/v3yqZq1VHqGKD96AZpNjyU/skincare-deals-banner-600x66/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"
The skin of men and women differ in areas like thickness, hydration, and oil production. That’s why facials tailored specifically to men’s skin exist.
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Find out how Botox works, how quickly it works, and what the possible side effects could be.
","url":"/articles/what-is-botox","title":"What Is Botox?"},{"guid":"c4ff7271-4fb5-4e6e-9b82-039dd8d9dce8","imageUrl":"//img.grouponcdn.com/seocms/gNeZgNdLGqic6m3vHxGbjs/Brow_extensions_hero-600x385/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"
Instead, they can fill in the gaps, enhance brow shape, and hide years of overtweezing.
","url":"/articles/eyebrow-extensions-cheat-sheet","title":"No, Eyebrow Extensions Won't Make Your Brows Really Long"}],"featuredArticles":[{"guid":"9f34d3dd-c0d0-4900-8ef9-9e7d059692e2","imageUrl":"//img.grouponcdn.com/seocms/4Ry1zEGN4Rp6gG8utSopv3o1CKSk/00_woman-using-cookware-on-stovetop_jpg-600x390/v1/c140x85.png","bodyColumns":"nine","vhp_desc":"
Our guide breaks down the benefits, materials, and cleaning methods for the different kinds of cookware you'll come across while shopping.
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Four natural beauty experts recommend their favorite natural skincare products for fine lines, acne, hyperpigmentation, and dryness.
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Microneedling isn’t as alarming as its name sounds. In reality, it’s becoming a popular method at med spas for helping smooth and firm skin.
","url":"/articles/what-is-microneedling","title":"Know Before You Go: Microneedling"}]}}}}}

value: This website is an American global e-commerce marketplace connecting subscribers with local merchants by offering activities, travel, goods and services in 15 countries. Based in Chicago, Groupon was launched there in November 2008, launching soon after in Boston, New York City and Toronto.


text:
Reinhold Joest - Wikipedia, the free encyclopedia
Despite never winning as a driver at Le Mans, Joest ended his driving career in style. After winning the 24 Hours of Daytona in a Porsche 935 and on the Nürburgring with his Porsche 908/3 Turbo in 1980 (both with Rolf Stommelen), he went on to win several German DRM races in 1981 with a mighty Po


text:
Microsoft Sharepoint Developer Support Specialist Salary in Raleigh, NC | Indeed.com
Microsoft Sharepoint Developer Support Specialist Salary in Raleigh, NC
Microsoft Sharepoint Developer Support Specialist in Raleigh, NC
$80,000 Average Microsoft Sharepoint Developer Support Specialist salaries for job postings in Raleigh, NC are 4% lower than average Microsoft Sharepoint Developer Support Specialist salaries for job postings nationwide.
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Microsoft Sharepoint Developer Support Specialist in Raleigh, NC $80,000

Microsoft Sharepoint Developer Support Specialist in Raleigh, NC $80,000

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value: This website is an American worldwide employment website for job listings launched in November 2004. It is a subsidiary of Japan's Recruit Co.


text: Re: Mega-List of Movies that are in question. PLEASE check to see if they're PD or Copyrighted.. Video-Cellar, are you around? That's what I was wondering if they were either valid at all. Can someone get a hold of Video-Cellar :D lol.This post was modified by jeffzkrazie on 2011-02-19 23:52:33 Terms of Use (31 Dec 2014)

value: This website is an American digital library with the stated mission of "universal access to all knowledge". It provides free public access to collections of digitized materials, including websites, software applications/games, music, movies/videos, moving images, and millions of books.


text:
Panel Reverses Pitts Suspension - The Washington Post
Panel Reverses Pitts Suspension
The Maryland Racing Commission reversed itself today and withdrew a five-day suspension meted out to State Racing Steward Clinton Pitts Jr. Tuesday.
Pitts was suspended by Commission Chairman Robert Banning for "using inappropriate language" when responding to another commissioner, Robert Furtick, over failure to scratch Dear Rossi from Saturday's third race.
The withdrawal of the suspension was not requested by Pitts or his attorney, Glen Bushell of Baltimore. Bushell said, "It appears that the commission may be having a change of heart and I will not comment on the matter until they make anything they decide official."
Todd Taylor, assistant attorney general representing the commission, said, "All I can say is that the commission withdrew-that is the word they used-the suspension of Pitts. The reasons are their own."
Asked of there were legal technicalities involved in the surprise decision, Taylor replied. "No the suspension was legal and its withdrawal is also legal. The Commission will meet again very shortly-within a few days. I would say-and would make their views on this and other matters know."
Earlier today, Pitts announced he would not be replaced for the term of the suspension even though it was to have begun today.
Furtick was asked before the announcement of the withdrawal if the commission had had a change of heart.
He declined comment and referred all questions to the assistant attorney general.
The suspension of Pitts was the first for a high racing official in Maryland.

value: This website is an American daily newspaper published in Washington, D.C. It is the most-widely circulated newspaper within the Washington metropolitan area, and has a large national audience. Daily broadsheet editions are printed for D.C., Maryland, and Virginia.


text:
Downregulation of ClpR2 leads to reduced accumulation of the ClpPRS protease complex and defects in chloroplast biogenesis in Arabidopsis. - PubMed - NCBI
Format: AbstractFormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListApplySend toChoose DestinationFileClipboardCollectionsE-mailOrderMy BibliographyCitation managerFormatSummary (text)Abstract (text)MEDLINEXMLPMID ListCSVCreate File1 selected item: 16766689FormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListMeSH and Other DataE-mailSubjectAdditional textE-mailDidn't get the message? Find out why...Add to ClipboardAdd to CollectionsOrder articlesAdd to My BibliographyGenerate a file for use with external citation management software.Create File
See comment in PubMed Commons belowPlant Cell. 2006 Jul;18(7):1704-21. Epub 2006 Jun 9.Downregulation of ClpR2 leads to reduced accumulation of the ClpPRS protease complex and defects in chloroplast biogenesis in Arabidopsis.Rudella A1, Friso G, Alonso JM, Ecker JR, van Wijk KJ.Author information1Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.AbstractPlastids contain tetradecameric Clp protease core complexes, with five ClpP Ser-type proteases, four nonproteolytic ClpR, and two associated ClpS proteins. Accumulation of total ClpPRS complex decreased twofold to threefold in an Arabidopsis thaliana T-DNA insertion mutant in CLPR2 designated clpr2-1. Differential stable isotope labeling of the ClpPRS complex with iTRAQ revealed a fivefold reduction in assembled ClpR2 accumulation and twofold to fivefold reductions in the other subunits. A ClpR2:(his)(6) fusion protein that incorporated into the chloroplast ClpPRS complex fully complemented clpr2-1. The reduced accumulation of the ClpPRS protease complex led to a pale-green phenotype with delayed shoot development, smaller chloroplasts, decreased thylakoid accumulation, and increased plastoglobule accumulation. Stromal ClpC1 and 2 were both recruited to the thylakoid surface in clpr2-1. The thylakoid membrane of clpr2-1 showed increased carotenoid content, partial inactivation of photosystem II, and upregulated thylakoid proteases and stromal chaperones, suggesting an imbalance in chloroplast protein homeostasis and a well-coordinated network of proteolysis and chaperone activities. Interestingly, a subpopulation of PsaF and several light-harvesting complex II proteins accumulated in the thylakoid with unprocessed chloroplast transit peptides. We conclude that ClpR2 cannot be functionally replaced by other ClpP/R homologues and that the ClpPRS complex is central to chloroplast biogenesis, thylakoid protein homeostasis, and plant development.PMID: 16766689 PMCID: PMC1488914 DOI: 10.1105/tpc.106.042861 [PubMed - indexed for MEDLINE] Free PMC ArticleShareImages from this publication.See all images (11)Free textFigure 1.Isolation and Complementation of a T-DNA Insertional Mutant for CLPR2.(A) The T-DNA insertion site is 7 bp upstream of the translational start codon of CLPR2. Exons are indicated in black. The fragment used for complementation is shown through primer annealing sites (arrows) as well as the introduced restriction sites. The T-DNA (clpr2-1) and (his)6 (R2:h) tags are not drawn to scale. Bar = 1 kb.(B) Homozygous clpr2-1 shows delayed development and a yellow/pale-green phenotype but can germinate and be maintained on soil. Younger leaves are paler with serrated leaves. The complemented line (clpr2-1/R2:h) shows a wild-type phenotype.(C) Semiquantitative RT-PCR profile for CLPR2 (top panels) and ACTIN2 (bottom panels) in leaves and roots of clpr2-1 and the wild type.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 2.Incorporation of Transgenic ClpR2:(his)6 into the 325-kD ClpPRS Complex.The incorporation of transgenic ClpR2:(his)6 into the 325-kD ClpPRS complex in the complemented clpr2-1 line was analyzed. Native stroma was obtained from purified intact chloroplasts and separated in triplicate first on CN-PAGE gels, followed by SDS-PAGE in the second dimension. Two 2D gels were blotted to PVDF membranes and probed with anti-polyHis serum (A), and the third gel was stained with Sypro Ruby (B).(A) Immunoblot of the bottom part (dotted square) of a CN-PAGE separation of stromal complexes from the complemented line clpr2-1/R2:h. The region where the Clp core complex subunits migrate is enclosed by a dotted ellipse.(B) CN-PAGE as in (A) but stained with Sypro Ruby. Dotted square and ellipse are as in (A); relative position of the anti-polyHis signal is represented by white ovals. The dotted lines indicate the transition from stacking to resolving gel in the first dimension.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 3.Native Stromal Proteome in clpr2-1.CN-PAGE separation and Sypro Ruby staining of 1 mg of stromal proteins from wild-type (A) and clpr2-1 (B) chloroplasts from fully developed rosettes. The identified proteins are indicated with spot numbers. Protein identities were determined by nanoLC-ESI-MS/MS in this study or for the wild type in a parallel and independent study (). For the complete data set, see Supplemental Table 1 online. Spot numbers 1 to 9 correspond to the ClpPRS complex. Spot numbers 10 to 31 correspond to the following proteins: 10, uridylyltransferase-related; 11, glyceraldehyde-3-phosphate dehydrogenase A-1,2 (GAPA-1,2); 12, Cpn60-α,β-1,2; 13, ferredoxin-dependent Glu synthase (Fd-GOGAT 1); 14, ClpC1,C2,D; 15, metalloproteases (M16) (AtPreP1,2); 16, lipoxygenase AtLOX2; 17, HSP90, elongation factor Tu-G (EF-G), and starch branching enzyme class II (SBEII); 18, cpHSP70-1,2 (DnaK homologues); 19, Transketolase-1 (TKL-1); 20, plastid phosphoglucomutase (PGM1); 21, fructose-bisphosphate aldolase-1,2 (SFBA-1,2); 22, fructose-bisphosphatase (FBPA); 23, monodehydroascorbate reductase (MDHAR); 24, phosphoglycerate kinase-2 (PGK-2); 25, phosphoglycerate kinase-1 (PGK-1); 26, annexin (AnnAt1); 27, sedoheptulose-bisphosphatase (SBPase); 28, GrpE-1; 29, thiazole biosynthetic enzyme (THI1); 30, 3-β-hydroxy-δ5-steroid dehydrogenase; 31, triosephosphate isomerase-1 (TPI-1); 32, Cpn21 (also Cpn20), carbonic anhydrase-1 (CA1); 33, peptidylprolyl isomerase ROC4.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 4.Quantitative Comparative Analysis of ClpPRS Complex in the Wild Type and clpr2-1 Using Differential Stable Isotope Labeling with iTRAQ.(A) Schematic representation of the isobaric iTRAQ tags used for the differential labeling. The tag consists of a balance group (28 or 31 D) and a reporter group (114 or 117 D), which is released upon fragmentation in the mass spectrometer.(B) Spots containing Clp subunits and the reference spot THI1 were sliced as four gel pieces as indicated (1 to 4) from 2D gels (CN-PAGE followed by SDS-PAGE) of the stromal proteome of the wild type and clpr2-1. Gel pieces were washed and digested with trypsin. Peptides were extracted with formic acid and differentially labeled with the iTRAQ reagents (i.e., 114 for wild-type and 117 for clpr2-1 samples). After pairwise (pairs of identical spot numbers) mixing of samples from the wild type and clpr2-1, the samples were cleaned up using C18 microcolumns. The eluates for each pair were analyzed by reverse-phase nanoLC-ESI-MS/MS. After mass spectral data processing, sequence information was used for protein identification, and the reporter ions (114 and 117) were used for peptide quantification. The experiment was performed with two independent biological replicates, with a label switch between the replicates to avoid a possible systematic bias due to the label. Within one biological replicate, duplicate gels for the wild type and for clpr2-1 were used to reduce variability. Each sample was analyzed twice by MS/MS.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 5.Quantitative Comparative Analysis of ClpPRS Complex in the Wild Type and clpr2-1 Using Differential Stable Isotope Labeling with iTRAQ.(A) An example of the MS/MS spectrum of a double-charged peptide (m/z = 599.84; IALQSPAGAAR; the y-ion series is indicated) belonging to the ClpR2 protein and the reporter ion pair at 114 and 117 generated in the collision cell of the mass spectrometer. Peptides from clpr2-1 were labeled with the heavy tag (117) and the wild type with the light tag (114). Inset 1 shows a close-up of the 114 to 117 region of this MS/MS spectrum, with a calculated ratio between the areas under the 114 and 117 peaks of 0.2. This shows that accumulation of ClpR2 in the mutant is fivefold lower than in the wild type (normalized to total stromal protein). Inset 2 shows a close-up of the 114 to 117 area for a peptide belonging to the THI1 protein in gel slice 4. In this case, the 114:117 ratio is 1.4, indicating that THI1 is 40% higher in the mutant than in the wild type (normalized on basis of total stromal protein). High-quality MS/MS spectra and 114:117 reporter ratios were obtained for 85 peptides belonging to the ClpP/R/S and THI1 proteins. A complete list of these peptides and reporter ion ratios is listed in Supplemental Table 2 online.(B) Summary of quantification of the different ClpP/R/S proteins accumulating in the ClpPRS complex displayed as ratios between clpr2-1 and the wild type. Standard deviations for the biological replicates are indicated. The black bars show uncorrected average ratios, and the gray bars show average ratios corrected for differences in protein loading, as determine by total spot volume per gel. Details for the peptides used for quantification are listed in Supplemental Table 2 online.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 6.Light, Confocal, and Transmission Electron Microscopy Analysis of Leaves of clpr2-1 and the Wild Type.(A) to (D) Light microscopy of transverse sections from young, expanding leaf blades of the wild type (A) and clpr2-1 (B) and of mature leaves of the wild type (C) and clpr2-1 (D).(E) and (F) Laser scanning confocal microscopy images of mesophyll cells of the wild type (E) and clpr2-1 (F). Only the red channel for chlorophyll autofluorescence is shown, collected at the same excitation intensities.(G) to (L) TEM analysis of the ultrastructure of chloroplasts from young expanding leaves of wild-type (G) and clpr2-1 plants (H) and from mature leaves of wild-type (I) and clpr2-1 (J) leaves. Close-ups of plastoglobules in chloroplasts of mature leaves of the wild type (K) and clpr2-1 (L).Bars = 25 μm in (A) to (D), 5 μm in (E) and (F), 1 μm in (G) to (J), and 0.2 μm in (K) and (L). UE, upper epidermis; LE, lower epidermis; PM, palisade mesophyll; SM, sponge mesophyll; S, stomata; V, vasculature.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 7.Oligomeric Soluble Cellular Proteomes of the Wild Type and clpr2-1.CN-PAGE separation and Sypro Ruby staining of 2 mg of total soluble proteins from fully developed rosettes of wild-type (A) and clpr2-1 (B) plants. The predominant chloroplast stromal components are the Rubisco large and small subunits (RbcL and RbcS, respectively). The extrachloroplastic 26S proteasome complex (ovals) and cytosolic Met synthase 1,2 (MS1, At5g17920; MS2, At3g03780) and vacuolar TGG1 (At5g20980) are indicated.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 8.Starch Accumulation and Starch Breakdown in Wild-Type and clpr2-1 Leaves.To determine the net balance of reduced carbohydrate production (supply) and consumption (demand), leaves of the wild type and clpr2-1were stained (by iodine) for starch accumulation at the end of the light or dark periods. (A) shows starch staining of the wild type and clpr2-1 of 6-week-old plants, and (B) shows starch staining of the wild type and clpr2-1 ∼1 week prior to bolting.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 9.Thylakoid Protein Profiles of Chloroplasts from Wild-Type and clpr2-1 Leaves and Identification by Mass Spectrometry.(A) Thylakoid proteins were purified from leaves of wild-type and clpr2-1 plants and were separated by tricine SDS-PAGE and stained with Sypro Ruby, and 100-μg proteins were loaded in each lane. Molecular markers are indicated. Bands that are numbered were excised and digested with trypsin, and peptides were extracted and analyzed by nanoLC-ESI-MS/MS. A complete listing of all identified proteins is given in Supplemental Table 3 online.(B) Sequence alignment of the N termini of LHCII1.1 and 2 (At1g29910 and At1g29920), LHCII1.3 (At1g29930), LHCII1.4 (At2g34430), LHCII1.5 (At2g34420), and LHCII.3 (At5g54270). Boxed residues indicate the peptides identified by MS/MS in clpr2-1, containing the predicted cTP cleavage site indicated by arrowheads. The most N-terminal peptides identified by MS/MS previously in extensive thylakoid proteome analyses of wild-type plants are underlined (; ). N termini determined by Edman sequencing reported in the literature and in SWISS-PROT are indicated as single boxed residues (see ).(C) MS/MS fragmentation of doubly charged precursor ion with m/z of 764.40. This is a 1526.79-D peptide AVNLSPAASEVLGSGR matching to LHCII-1.1 and 2 (At1g29910 and At1g29920) with a Mowse score of 75.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 10.Immunoblot Analyses of Chloroplast Protein Populations.To evaluate the effect of reduced ClpPRS protease complex accumulation on the major thylakoid protein complexes, as well as proteases and chaperones in stroma and thylakoid, a protein gel blot analysis was performed. Titrations (indicated as 1x, 2x, 3x, and 10x) of thylakoid proteins ([A] and [B]) or total soluble protein extracts (C) from the wild type, clpr2-1, and the complemented line were separated by SDS-PAGE and blotted onto PVDF membranes.(A) Membranes were probed with antibodies generated against different proteins of PSI and II, the cytochrome b6f complex, F-type ATP-synthase (Group I), and different chloroplast chaperones and proteases (Group II). Group I proteins are as follows: D1/2, the D1 and D2 proteins of PSII; PsbS, a unique antenna protein in PSII; Cytf, the 32-kD heme binding cytochrome f protein in the cytochrome b6f complex; PSIA/B, the reaction center A and B proteins of PSI; PSIC/E, PsaC and PsaE of PSI; CF1, the peripheral α- and β-subunits of the thylakoid ATP synthase complex. The protein ratios in clpr2-1/wild type were determined by two or three independent experiments and were consistently close to one. Group II proteins are as follows: FtsH2 and 5, thylakoid members of the FtsH Zn metalloprotease family; SppA, an ATP-independent light-induced Ser-type thylakoid protease; ClpC1,2, Hsp100 chaperones in the Clp family. Protein ratios in clpr2-1/wild type were quantified in two to three independent experiments and were on average 2.7 (FtsH5), 2 (FtsH2), 10 (SppA), and >10 (ClpC1 and C2).(B) Protein gel blot of purified thylakoid membrane proteomes of the wild type, clpr2-1, and the complemented line clpr2-1/R2:h, probed with anti-PsaF. A strong cross-reacting band at ∼25 kD can be seen in clpr2-1 but not in the wild type or clpr2-1/R2:h. This band is the unprocessed PsaF protein (marked as pPsaF and indicated with a solid arrow). The bottom panel shows the response of antiserum directed against thylakoid protease SppA in the wild type, clpr2-1, and clpr2-1/R2:h. Lanes were loaded with 10, 30, and 100 μg of thylakoid protein (1x, 3x, and 10x, respectively).(C) Protein gel blot of total soluble leaf proteomes of the wild type and clpr2-1. Equal protein concentrations (1x and 2x) were loaded for the wild type and clpr2-1 and probed with antisera directed against Cpn60, Hsp70, ClpC1, and ClpC2. Protein ratios in clpr2-1/wild type were quantified in one to three independent experiments and were on average 6 (Cpn60), 2 (HSP70), 2 (ClpC1), and 7 (ClpC2).Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Figure 11.Summary of the Analysis of clpr2-1.Simplified summary of the consequences of reduced CLPR2 expression for chloroplast protein homeostasis. Arrows indicate the differential response, with thickness of the arrow correlating to the fold change. Car., carotenoid/thylakoid protein ratio; PG, plastoglobule; ZnMP, Zn2+ metalloprotease.Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in ArabidopsisPlant Cell. 2006 July;18(7):1704-1721.Publication Types, MeSH Terms, SubstancesPublication TypesResearch Support, U.S. Gov't, Non-P.H.S.MeSH TermsAmino Acid SequenceArabidopsis*/cytologyArabidopsis*/enzymologyArabidopsis*/physiologyArabidopsis Proteins/geneticsArabidopsis Proteins/metabolism*Chloroplasts/metabolism*Chloroplasts/ultrastructureDown-RegulationElectron Transport Chain Complex Proteins/geneticsElectron Transport Chain Complex Proteins/metabolismEndopeptidase Clp/geneticsEndopeptidase Clp/metabolism*Gene Expression Regulation, Enzymologic*Gene Expression Regulation, Plant*HomeostasisMolecular Chaperones/metabolismMolecular Sequence DataMultienzyme ComplexesPhenotypePhotoperiodPhotosystem II Protein Complex/physiologyPigments, Biological/metabolismPlant Leaves/metabolismPlant Leaves/ultrastructurePlants, Genetically ModifiedPlastids/enzymologySequence AlignmentStarch/metabolismThylakoids/chemistryThylakoids/metabolismThylakoids/ultrastructureSubstancesArabidopsis ProteinsElectron Transport Chain Complex ProteinsMolecular ChaperonesMultienzyme ComplexesPhotosystem II Protein ComplexPigments, BiologicalStarchEndopeptidase ClpLinkOut - more resourcesFull Text SourcesHighWirePubMed CentralOther Literature SourcesCOS Scholar UniversePubMed Commons home

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Born in Virginia, Henican was reared in New Orleans, Louisiana, where his father, C. Ellis Henican, Jr. (1933-2015), a Tulane University Law School graduate, practiced law for fifty-seven years until his death at the age of eighty-one. C. Ellis Henican also taught securities regulation at Loyola University New Orleans College of Law. Henican's mother was the former Patricia McGraw. One of his aunts is Peggy Henican Wilson, a Republican former member of the New Orleans City Council.
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DENVER, Aug ... The film features interviews with Waltrip and Dale Earnhardt Jr ... Based on the New York Times bestselling book, "In the Blink of an Eye. Dale, Daytona, and the Day that Changed Everything" by Michael Waltrip and Ellis Henican, "Blink of an Eye" focuses on Waltrip and his star-crossed friendship with iconic driver Earnhardt Sr ... in third ... .......
LOS ANGELES--(BUSINESS WIRE)--Aug 7, 2019--. The latest work of groundbreaking filmmaker and multi Emmy-winning Director, Producer and Writer, Paul Taublieb of TAUBLIEB Films, is the feature documentary Blink of an Eye ... The film is inspired by Waltrip and Ellis Henican’s 2011 New York Times best-selling book, In the Blink of an Eye ... CONTACT ... KEYWORD....
If the book is sold, it will be co-written by former Fox News liberal pundit Ellis Henican, who recently co-wrote former New Jersey Gov ... In a phone call on Wednesday, Henican confirmed that although nothing has been sold yet, the governor has definitely taken “initial” steps toward publishing a book....
Is it a pre-election gift to the Bush campaign from al-Qaida? Or is it the Republicans' long-awaited October surprise?. Ellis Henican. 10/29/04 "Newsday" -- A 75-minute videotape, featuring a scary-looking fellow in sunglasses and a Palestinian headscarf, warning that blood will "run red" in the streets of America. Yikes! ... Azzam ... "Kerry is my man." ... HOME....
Let Me Finish review: Everybody hates Chris Christie - and he hates Jared Kushner
Let Me Finish review. Everybody hates Chris Christie  and he hates Jared Kushner. The former New Jersey governor spares Donald Trump but lashes out at everyone else ... Lloyd Green ... -snip- ... With the assistance of Ellis Henican, a veteran of Newsday and talk radio, Christie paints a portrait of Kushner as callow, smarmy, entitled and way over his skis ... ....
Chris Christie failed to a win a single delegate in his quest for the Republican presidential nomination in 2016, and garnered less than 1% of the votes cast in the GOP primary ... With the assistance of Ellis Henican, a veteran of Newsday and talk radio, Christie paints a portrait of Kushner as callow, smarmy, entitled and way over his skis ... ....
Ellis Henican has been tapped as the “as told to author” for a book by Ballantine on the daunting rescue of the soccer team and coach trapped in a flooded cave in Thailand this summer ... We have exclusive cooperation from all the key players,” said Henican ... “The kids and the coach are super spiritual,” noted Henican....
What do you say when someone dies you never really liked? Very few Americans are wrestling with that quandary today when it comes to John McCain. John McCain was one of the very most beloved people anywhere in public life ... You know, before Donald Trump ... Ellis Henican is the author of a dozen books including TRUMPITUDE ... Join Ellis on Twitter @henican....
Why set the bar high? You’ll only fall short and leave people disappointed ... But the usually boastful American president certainly made no grand promises ... “I’ve been nice to him. He’s a competitor ... Metro columnist Ellis Henican is the author of a dozen books including “TRUMPITUDE. The Secret Confessions of Donald’s Brain.” Join Ellis on Twitter @henican....
Q. What’s the difference between innocent children trapped in a cave in Thailand and innocent children trapped in an immigrant jail in the United States? A. In Thailand, rescuers are hurrying to free the innocents ... Meanwhile, at the U.S ... Metro columnist Ellis Henican is the author of a dozen books including "TRUMPITUDE ... Join Ellis on Twitter @henican....
Spain’s Gerard Pique and Sergio Ramos were both slammed down by a mob of Russian defenders. Yet the referee was never instructed to review the encounter and waved the play on. Russia went on to win the game on penalties ... Metro columnist Ellis Henican is the author of a dozen books including TRUMPITUDE ... Join Ellis on Twitter @henican .......
Democrats are howling. Texans are howling. Many Republicans are howling, too ... Mike Pence had better find a new Bible verse to defend this latest abomination, and it won’t be Matthew 25.40 ... “Mrs ... It certainly won’t get Trump’s wall built ... Metro columnist Ellis Henican is the author of a dozen books including "TRUMPITUDE ... Join Ellis on Twitter @henican. ....
Too bad international diplomacy isn’t as simple as signing a presidential pardon. If that were true, Donald Trump could be hailed in Singapore today as the 21 st century’s Dag Hammarskjöld ... No rules. No limits ... Metro columnist Ellis Henican is the author of a dozen books including "TRUMPITUDE ... Join Ellis on Twitter @henican .......

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Phone: (814) 231-3100
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Many restaurants closed in the Summer. - Review of Negril Cliffs, Negril, Jamaica - TripAdvisor
Many restaurants closed in the Summer. - Negril Cliffs
“Many restaurants closed in the Summer.”
See more tours on Viator Ranked #3 of 56 things to do in Negril 1,359 Reviews
87 helpful votes “Many restaurants closed in the Summer.”
If staying on the cliffs in the summer many of the recommended restaurants on tripadvisor are closed. Even highly rated ones. Serious Chicken and Hungry Lion to name two. But it is peaceful and secluded and just the ticket if you're wanting to get away from it all without being surrounded by hundreds of tourists. If your over 40 and married you probably need quality time together and the cliffs are the spot. It's romantic as you could hope for, go for it.
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Dutch first English first French first German first Italian first Japanese first Portuguese first Russian first Spanish first Swedish first Any JoeFat
23 helpful votes “Cliff divers, Nigril, Jamaica”
It was worth the trip out to see. Just remember one thing, the 3 divers (the day we were there) do not jump until they have a $20.00 in tips in their possession. If you are on a schedule time, you may have to pony up all $20.00 to see them dive. It was the end of the day for us and very few other tourist around so you got to do what you have to do. It's a good hour and half from Montaga Bay and we needed to get back. Pay up and watch the show. It was worth it. They drive so slow in Jamaica.
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This review is the subjective opinion of a TripAdvisor member and not of TripAdvisor LLC. tmhmichael
6 helpful votes “ricks was fun”
crazy jumping of cliffs exciting cab ride to say the least just close your eyes you will make it nice dinner at ivans very romantic at sunset could see all the boats and cruises
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25 helpful votes “Awesome caves to explore & play in crystal clear waters!”
We originally headed out on a catamaran tour to Rick's, but it was packed in that cove with three or four other catamarans waiting to get in. So our crew took us back about a mile towards 7 mi beach to another set of caves that were so much better! They let us off to play & explore the area & we were told that once upon a time, a bar had been set up inside one of the caves we were at due to the water's natural shaping of the cave walls inside. So much more fun than we would've had at Rick's, waiting in lines, having to tip, etc...So grateful we got to do this instead!
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5 helpful votes “Rick's Cafe? Overrated...”
We took a boat down to Rick's Cafe on the cliffs. We swam in from the boat as there is little places for larger boats to dock. We climbed the stairs while being lured into the caves by the locals. (No way.) We jumped from the 30-35' "cliff" and then swam back. We were told by locals we trusted that the food is terrible and overpriced so we steered clear. Don't waste your time unless you really need to tell your friends you jumped.
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