text:
Patent US5241081 - Treating dental hard substances - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsThe invention relates to new N-alkyl-N-(meth)acryloyloxyalkylcarboxamides of aromatic carboxylic acids and aromatic carboxylic acid anhydrides, their preparation and formulations of these compounds for use as adhesives for the treatment of dental hard substance....http://www.google.com/patents/US5241081?utm_source=gb-gplus-sharePatent US5241081 - Treating dental hard substancesAdvanced Patent SearchPublication numberUS5241081 APublication typeGrantApplication numberUS 07/743,535Publication dateAug 31, 1993Filing dateAug 9, 1991Priority dateAug 15, 1990Fee statusLapsedAlso published asDE4039440A1, DE59101267D1, EP0471252A1, EP0471252B1, US5294646Publication number07743535, 743535, US 5241081 A, US 5241081A, US-A-5241081, US5241081 A, US5241081AInventorsMichael Muller, Wolfgang Podszun, Werner Finger, Jens WinkelOriginal AssigneeBayer AktiengesellschaftExport CitationBiBTeX, EndNote, RefManPatent Citations (4), Non-Patent Citations (14), Referenced by (5), Classifications (33), Legal Events (5) External Links: USPTO, USPTO Assignment, EspacenetTreating dental hard substances
US 5241081 AAbstract
1. N-Alkyl-N-(meth)acryloyloxyalkylcarboxamides of the formula (I) ##STR24## in which X represents an N-alkyl-N-(meth)acryloyloxyalkylcarboxamide of the formula ##STR25## wherein R1 denotes hydrogen or methyl,R2 denotes a divalent aliphatic radical (C2 -C6) and R3 denotes a monovalent aliphatic radical (C1 -C4), Y represents COOH, it also being possible for adjacent groups Y to be linked to form an anhydride group (--CO--O--CO--) Z represents H, X or, if Y denotes COOH, Y and Ph represents a tri- or tetrasubstituted benzene ring (1,2,3-/1,2,4- or 1,2,4,5-substitution) or a tri- or tetrasubstituted naphthalene ring (1,2,6-/1,4,5-/2,3,6-/1,4,5,8- or 2,3,6,7-substitution). 2. N-Alkyl-N-(meth)acryloyloxyalkylcarboxamides of the formula (II) ##STR26## in which R1 denotes hydrogen or methyl,R2 denotes a divalent aliphatic radical (C2 -C6) and R3 denotes a monovalent aliphatic radical (C1 -C4), Z' denotes hydrogen or COOH and Ph represents a tri- or tetrasubstituted benzene ring (1,2,3-/1,2,4- or 1,2,4,5-substitution) or a tri- or tetrasubstituted naphthalene ring (1,2,6-/1,4,5-/2,3,6-/1,4,5,8- or 2,3,6,7-substitution). 3. N-Alkyl-N-(meth)acryloyloxyalkylcarboxamides of the formula (III) ##STR27## in which R1 denotes hydrogen or methyl,R2 denotes a divalent aliphatic radical (C2 -C6) and R3 denotes a monovalent aliphatic radical (C1 -C4), Z' denotes hydrogen or COOH and Ph represents a benzene ring which is tri- or tetrasubstituted (1,2,3-/1,2,4- or 1,2,4,5-substitution) or a tri- or tetrasubstituted naphthalene ring (1,2,6-/1,4,5-/2,3,6-/1,4,5,8- or 2,3,6,7-substitution). 4. N-Alkyl-N-(meth)acryloyloxyalkylcarboxamides of the formula (IV) ##STR28## in which R1 denotes hydrogen or methyl,R2 denotes a divalent aliphatic radical (C2 -C6) and R3 denotes a monovalent aliphatic radical (C1 -C4), Z' denotes hydrogen or COOH and Ph represents a tri- or tetrasubstituted benzene ring (1,2,3-/1,2,4- or 1,2,4,5-substitution) or a tri- or tetrasubstituted naphthalene ring (1,2,6-/1,4,5-/2,3,6-/1,4,5,8- or 2,3,6,7-substitution). 5. Compositions containing carboxamides according to claim 1, a solvent and if appropriate an initiator.
R3 denotes a monovalent aliphatic radical (C1 -C4), in an organic solvent at between -30� and 110� C., if appropriate in the presence of a base, and if appropriate the resulting product is hydrolysed with water at between 5� and 100� C.
A suitable temperature range for the reaction of the amine VII is between -30� and 110� C. This reaction is preferably carried out at between -10� and 50� C., and particularly preferably at between -5� and 30� C. Inorganic or organic bases can additionally be used in the process according to the invention.
Preferred inorganic bases are the weakly basic carbonates and bicarbonates of sodium and potassium. Preferred organic bases are tertiary amines, triethylamine and pyridine being particularly preferred. The bases are employed in an equimolar to five times the molar amount in respect of the anhydride of the formula (V) or (VI) employed, a 2- to 3-fold molar excess being preferred. The organic bases in this manner additionally act as solubilising agents. Nevertheless, it may also be advantageous to carry out the process according to the invention in a supersaturated solution of the anhydride (V) or (VI), that is to say in a dispersion, since the reaction product is more readily soluble than the anhydride and the anhydride is consequently dissolved as the reaction progresses. To prepare the derivatives with adjacent carboxylic acid groups of the general formula (I), the anhydrides obtained in the above process are hydrolysed at temperatures between 5� and 100� C., and preferably between 20� and 50� C. This can be effected either after isolation of the anhydrides of the general formula (I) or without isolation, by direct hydrolysis of the reaction mixture. To carry out this hydrolysis, an equimolar amount, but preferably more than ten times the molar amount, of water is added. It is a particular feature of the process according to the invention that it may be advantageous that the water added does not have to be miscible with the solvent used in the reaction. Precisely hydrolysis in a two-phase mixture is preferred, since in this case the most reactive anhydride group and not the amide and ester bonds in the compound (I) according to the invention is attacked in a targeted manner.
--B--NH3 D
Preparation of carboxamides according to the invention
A solution of 185.27 g (1.000 mol) of N-tert.-butyl-2-aminoethyl methacrylate in 250 ml of dry methylene chloride was added dropwise to a solution of 210.57 g (1.000 mol) of benzenetricarboxylic acid anhydride chloride in 650 ml of dry methylene chloride and 202.38 g (2.000 mol) of dry triethylamine at -5� C., while stirring. After the mixture had been stirred at room temperature for three hours, the pale-coloured solid which had precipitated was filtered off with suction and the filtrate was extracted with water and dried.
200 ml of water were added to 700 ml of the methylene chloride solution from Example 1 containing 218.03 g (0.607 mol) of the anhydride (VIII) and the mixture was refluxed with vigorous stirring for 22 hours at 41� C. After drying and stabilising the organic phase with 100 mg of 2,6-di-tert.-butylcresol it was able to be concentrated to give 181.00 g (79% of theory) of a pale-coloured highly viscous oil of trimellitic acid N-tert.-butyl-N-methacryloyloxyethylamide (IX). The oil obtained solidified on cooling and was able to be further purified by being extracted from a methylene chloride solution using aqueous sodium carbonate solution, acidifying the aqueous phase with dilute hydrochloric acid and re-extracting the product (IX) in a methylene chloride phase. Melting point: 74� C.
A solution of 370.54 g (2.000 mol) of N-tert.-butyl-2-aminoethyl methacrylate in 370 ml of dry methylene chloride was added dropwise to a suspension of 218.12 g (1.000 mol) of pyromellitic acid dianhydride, 750 ml of dry methylene chloride and 505.95 g (5.000 mol) of dry triethylamine, with vigorous stirring. A brown solution was formed during this procedure, while heating gently, and was stirred at 41� C. for one hour, poured into 1 l of water and acidified with half-concentrated sulphuric acid.
A solution of 185.27 g (1.000 mol) of N-tert.-butyl-2-aminoethyl methacrylate in 250 ml of xylene was added to a mixture of 218.12 g (1.000 mol) of pyromellitic acid dianhydride, 171.50 g (1.200 mol) of triethylamine and 2200 ml of xylene at room temperature, while stirring, and the mixture was heated at 50� C. for one hour. The mixture was filtered, the filtrate was poured into ice-water and, for hydrolysis, the mixture was acidified with half-concentrated sulphuric acid and stirred for 30 minutes. The precipitate which had separated out was filtered off with suction and recrystallised from ethyl acetate to give 232.05 g (about 55% of theory) of a white solid which, in addition to the pyromellitic acid (N-tert.-butyl-N-methacryloyloxyethylamide) (XII), contained a relatively small amount of the double reaction products (X) and (XI) of Example 3.
A solution of 185.27 g (1.000 mol) of N-tert.-butyl-2-aminoethyl methacrylate in 250 ml of methylene chloride was added dropwise to a mixture of 192.13 g (1.000 mol) of benzenetricarboxylic acid anhydride, 500 ml of dry methylene chloride and 303.57 g (3.000 mol) of dry triethylamine at room temperature, while stirring. After the mixture had been stirred at 41� C. for 4 hours, it was poured into 3 l of water, the mixture was acidified with half-concentrated sulphuric acid and the organic phase was separated off. The aqueous phase was extracted with methylene chloride and, after drying and stabilisation with 350 mg of 2,6-di-tert.-butylcresol, the combined organic phases were concentrated to give 284.60 g (78% of theory) of a beige-coloured solid of a mixture of the two isomers (XIII) and (XIV) of benzenetricarboxylic acid mono-(N-tert.-butyl-N-methacryloyloxyethylamide).
Preparation of the formulations for use as an adhesive
Use test, bonding strength
The activity and suitability of the adhesives (Examples 6-10) is checked by determination of the shear bonding strength to dentine. Human teeth which have been kept in 1% strength chloramine solution for a maximum of three months after the extraction are used. Before being used in the test, the teeth are kept in physiological saline solution for at least three and not more than ten days, after thorough washing under running water. On the day before their use in the bonding test, the teeth are embedded individually, lying on an approximal side, with epoxy resin (�LEKUTHERM X 20, hardener T 3) in cylindrical rubber moulds of 25 mm diameter and 12 mm height. The teeth are ground by wet grinding on SiC papers of grains 240, 320, 400 and finally 600 to the extent that an adequately large dentine surface close to the enamel is exposed for binding to a cylinder of plastic of 3.5 mm diameter. After rinsing with deionised water and drying in a stream of air, the conditioning solution �GLUMA 1 Cleanser is applied with a cotton-wool pellet using a rubbing movement for 30 seconds and the teeth are rinsed with water and dried, before the adhesive is applied with a brush, left on the surface for 30 seconds and then dried thoroughly in a stream of compressed air. One drop of �GLUMA 4 Sealer is then applied and blown into a thin layer with compressed air. The sample pretreated in this way is firmly clamped in a clamping device under a divisible Teflon mould having a cylindrical receptacle 3.5 mm wide and 1 mm in height. The cylindrical mould is then filled with the plastic filling material �PEKAFILL (U) using a syringe, and the filling material is covered with a strip which is impermeable to O2 and activated for 60 seconds under the supported light discharge opening of a �TRANSLUX CL (Kulzer) polymerisation lamp. The sample is then immediately removed from the holder. The Teflon mould is removed and the sample is kept in warm water at 23� C. for 15 minutes, until shearing stress is initiated, this being effected with the aid of a pressure piston parallel to and close to the surface of the embedded tooth at an advanced speed of 1 mm/minute until separation occurs. The shear bonding strength to the dentine is the quotient of the pressure and contact area on the tooth, and is in each case determined on 5 samples and stated as the mean value and standard deviation thereof.
Use test, tooth cavity
The extracted teeth are subjected to wet grinding on an undamaged aproximal side on SiC paper of grains 240, 320, 400 and 600 until a sufficiently large area of dentine is exposed for accommodation of a cylindrical cavity about 3 mm wide. The cavity is prepared down to a depth of about 1.5 mm using customary dentistry preparation diamonds of medium grain size with copious cooling with water, and is then rinsed out with water and dried. The cavity is cleaned with an impregnated cotton-wool pellet for 30 seconds as in the preceding example and is then washed out and dried, before the adhesive is brushed on, left for 30 seconds and finally dried. The �GLUMA 4 Sealer is then applied The excess is carefully removed with compressed air before the cavity is filled with the plastic filling material �PEKAFILL (U) using a syringe. The excess is covered with a strip which is impermeable to O2 before activation (60 seconds) using the �TRANSLUX CL (Kulzer) photopolymerisation apparatus. Immediately after the polymerisation, the filled tooth is kept in warm water at 23� C. for 15 minutes. Thereafter, the excess is removed by grinding on moist SiC paper of grains 400 and 600. During this operation, about 0.1 mm of the height of the cavity is worn away. The tooth is rinsed with water, dried in a stream of air and immediately inspected under a reflected light microscope at 500-fold magnification. The maximum width of any gap present at the edge is measured with the aid of a screw eyepiece micrometer. The average maximum gap width of in each case 5 fillings is stated as the measurement value. Microscopic examination of individual teeth was in all cases concluded in less than 10 minutes. It was thus ensured that the gaps measured at the edges had not formed or were not influenced in width by dehydration of the dentine.
Fillings which were exclusively free from gaps at the edges were even obtained by the method described using the formulation according to Example 6. The shear bonding strength was determined formally as 12.1�3.5 N/mm2, the fracture taking place in the dentine or plastic, as mentioned above, that is to say no adhesive failure being achievable.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS4148988 *Jun 19, 1978Apr 10, 1979Mitsui Petrochemical Industries Ltd.Dental adhesive and undervoating for paintsDE3510962A1 *Mar 26, 1985Oct 10, 1985G C Dental Ind CorpMit zahnunterlagen verbindbare massenEP0266220A2 *Oct 30, 1987May 4, 1988Mitsui Petrochemical Industries, Ltd.Curable compositionEP0377072A1 *Apr 13, 1989Jul 11, 1990Den Mat CorporationAdhesive bonding of acrylic resins, especially in dentistry* Cited by examinerNon-Patent CitationsReference1 *Adhesive Bonding to Dentin, vol. 61, No. 9, pp. 1070 1076.2Adhesive Bonding to Dentin, vol. 61, No. 9, pp. 1070-1076.3 *Computer printout of CAS online.4Computer printout of CAS-online.5 *Dental Materials Journal 7(2): 141 150, 1988.6Dental Materials Journal 7(2): 141-150, 1988.7 *Dirass Report, E 9 4, Jul., 1987, Dia Research Institute, Inc. Biocompatible Materials for the Oral Cavity .8Dirass Report, E 9-4, Jul., 1987, Dia Research Institute, Inc. "Biocompatible Materials for the Oral Cavity".9 *Journal of Biomedical Materials Research, vol. 18, 1089 1103 (1984).10Journal of Biomedical Materials Research, vol. 18, 1089-1103 (1984).11 *Kataoka et al, CA 105 200516w (1986).12Kataoka et al, CA 105-200516w (1986).13 *Proc. Natl. Sci. Counc. ROC(B), vol. 8, No. 2, 1984, pp. 187 192.14Proc. Natl. Sci. Counc. ROC(B), vol. 8, No. 2, 1984, pp. 187-192.* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS5338773 *Apr 19, 1993Aug 16, 1994Dentsply Research & Development Corp.Dental composition and methodUS5760101 *Mar 7, 1997Jun 2, 1998Heraeus Kulzer GmbhDerivatives of aromatic carboxylic acids from aromatic carboxylic acid anhydrides and hydroxy(meth) acrylates and formulations thereofUS5808104 *Jul 23, 1997Sep 15, 1998Heraeus Kulzer GmbhPolymerizable aromatic carboxylic acids and carboxylic acid anhydrides with cyclic carbonate groups and formulations thereofUS5962703 *Feb 21, 1997Oct 5, 1999Ivoclar AgDental adhesiveUS6177534Dec 17, 1998Jan 23, 2001The United States Of America As Represented By The Secretary Of CommerceSilylated resins and the synthesis thereof* Cited by examinerClassifications U.S. Classification549/232, 562/443, 560/129, 562/450, 562/442, 526/318, 560/183, 526/936, 526/272, 560/199, 560/196, 560/180, 526/269, 106/35, 526/306, 526/271, 549/557, 562/444, 549/244, 526/215, 549/236International ClassificationC07C233/69, A61C13/23, A61K6/00, C09J4/02, C07D307/89, C07D307/92Cooperative ClassificationY10S526/936, C07D307/89, C07C233/69European ClassificationC07D307/89, A61K6/00B, C07C233/69Legal EventsDateCodeEventDescriptionNov 11, 1997FPExpired due to failure to pay maintenance feeEffective date: 19970903Aug 31, 1997LAPSLapse for failure to pay maintenance feesApr 8, 1997REMIMaintenance fee reminder mailedMar 25, 1996ASAssignmentOwner name: HERAEUS KULZER GMBH & CO. KG, GERMANYFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BAYER AKTIENGESELLSCHAFT;REEL/FRAME:007869/0870Effective date: 19960221Aug 9, 1991ASAssignmentOwner name: BAYER AKTIENGESELLSCHAFT A CORP. OF GERMANY, GERFree format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:MULLER, MICHAEL;PODSZUN, WOLFGANG;FINGER, WERNER;AND OTHERS;REEL/FRAME:005813/0198;SIGNING DATES FROM 19910607 TO 19910612RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services


text:
Booking.com : Oban - hostely. Hostely v destinácii Oban, Veľká Británia.
hostely v meste: Oban
3 hostí zo Slovenska vysoko ohodnotilo destináciu Oban v kategórii príroda! Viac aktivít v destinácii Oban
Hľadáte niečo vynikajúce? Destinácia Oban hostí veľmi zaujala.
Hľadať hostely v meste Oban
v destinácii Oban Zobraziť mapu
Viac ubytovaní v destinácii Oban:
Dobré 620 hodnotení
The Regent Hotel is prominently situated at the head of Oban's north pier and is close to the shopping centre. „Place was extremely nice and clean , will be coming back again !!“
In the picturesque town of Oban, this Victorian guest house has bright, spacious en-suite rooms with free WiFi. Bars and restaurants are just 2 minutes’ walk away, and there is free on-site parking. „Everything was good about this accommodation, very… very comfortable. Will for sure stay here again.“
Priemerné 401 hodnotení
Located on the seafront, Oban Youth Hostel offers rooms with private toilets and showers, and is just 10 minutes’ walk from Oban rail and bus stations. „hostel s príjemnými priestormi a milým personálom … izbe a pomerne vysoká vybavenosť hostela poteší.“
Set in the small fishing town of Oban, Corran House Hostel is part of a Victorian terrace with scenic views across the bay to the Isle of Kerrera and the hills of Mull. „I liked it because i felt like home there, nice, c…. Nice kitchen, fully equipped, defo will go back“
Veľmi dobré 517 hodnotení
Situated less than 10 minutes’ walk from Oban’s famous McCaig’s Tower, The Ranald Hotel is set in the heart of Oban town centre and just off the main esplanade. „Comfortable, quiet, nicely decorated clean rooms. …otel. Definitely would stay again if ever back.“
Všetky hostely v destinácii Oban
Hotely v meste: Oban
157922,155918,158924,159959,155713,158698,158626,159854,154521,158924|1,150336,153097,157017,159490,159067,155568,151022,156018,155980,153695,158966

type: global

value: This website is a Dutch online travel agency for lodging reservations & other travel products, and a subsidiary of Booking Holdings. It is headquartered in Amsterdam.The website has over 28 million listings.


text:
Club Humidor in San Antonio, Texas with Reviews & Ratings - YP.comFind People•Advertise With UsBrowseFind PeopleFeatured CollectionsRestaurantsAutomotiveHome ImprovementHealth & WellnessLegalAdvertise with usWhat do you want to find?Search by business name, or keywordnearWhere? Address, ZIP Code, or NeighborhoodMy current locationSearchWelcome mybookProfileReviewsPhotosSign OutSign In•JoinHomeSan Antonio, TXClub HumidorSan Antonio Club HumidorSort:Most RelevantMost RelevantDistanceRatingName (A - Z)FiltersFiltersMore NeighborhoodsAvenida GuadalupeBabcock NorthCadillac DriveCastle HillsCotton CrossingDowntown San AntonioEast SideFar North CentralFar West SideFrench CreekHarlandaleHelotesHeritageHighland HillsHillcrestHills Of Park NorthHills of Park NorthJudsonKing WilliamLanda ParkLandmarkLeon ValleyMission RidgeNorth CentralNortheast San AntonioNorthwest SideThousand OaksUptown BroadwayUptown LoopWest SideDoneClear AllMore CategoriesAdult BookstoresAdult Novelty StoresAdult Video StoresBeer & AleCigar & Cigarette Accessories-Wholesale & ManufacturersCigar, Cigarette & Tobacco DealersCigar, Cigarette & Tobacco-Wholesale & ManufacturersClothing StoresClothing-Collectible, Period, VintageCollectiblesConsumer ElectronicsDVD Sales & ServiceElectronic InstrumentsGeneral Merchandise-WholesaleGift ShopsGrocery StoresHome Health Care Equipment & SuppliesHookah BarsIncenseKnives-MachineLingerieLiquor StoresMassage TherapistsNovelties-Wholesale & ManufacturersPipes & Smokers ArticlesSkateboards & EquipmentSmokers Information & Treatment CentersTobaccoWineDoneClear AllCategoryCigar, Cigarette & Tobacco DealersPipes & Smokers ArticlesTobaccoMore »FeaturesBook A TableCouponsOrder OnlineNeighborhoodsNorthwest SideEast SideFar North CentralMore »Go to List ViewSearch This Area1. Club Humidor(2)Cigar, Cigarette & Tobacco Dealers7074 San Pedro AveSan Antonio, TX 78216(210) 824-1209WebsiteMore Info2. Club HumidorDirections(1)Cigar, Cigarette & Tobacco Dealers11745 W Interstate 10San Antonio, TX 78230(210) 558-7700WebsiteDirectionsMore Info3. Club HumidorDirections(2)Cigar, Cigarette & Tobacco Dealers2235 Thousand Oaks DrSan Antonio, TX 78232(210) 828-1261WebsiteDirectionsMore Info4. Club HumidorDirections(1)Pipes & Smokers Articles12003 Huebner RdSan Antonio, TX 78230(210) 558-3022WebsiteDirectionsMore Info5. Club HumidorDirections(1)Pipes & Smokers Articles204 Alamo Plz Ste JSan Antonio, TX 78205(210) 472-2875DirectionsMore Info6. The HumidorDirectionsCigar, Cigarette & Tobacco Dealers22806 US Highway 281 NSan Antonio, TX 78258(210) 404-0707WebsiteDirectionsMore Info7. The Republic Cigar Humidor & LoungeDirectionsCigar, Cigarette & Tobacco Dealers2336 Gruene Lake DrNew Braunfels, TX 78130(830) 626-2525WebsiteDirectionsMore Info8. San Antonio Vape ClubDirectionsConsumer ElectronicsGift Shops7870 Culebra Rd. Ste. 24San Antonio, TX 78251(210) 689-0787DirectionsMore Info9. Callaghan Smoke Shop IncDirections(3)Cigar, Cigarette & Tobacco Dealers4310 Callaghan RdSan Antonio, TX 78228(210) 434-2038DirectionsMore Info10. SA VAPORSDirections(3)Pipes & Smokers Articles14910 Nacogdoches Rd, #101San Antonio, TX 78247(210) 646-8273WebsiteDirectionsMore Info11. Your Vaping StoreDirectionsGift Shops8015 Bandera Rd Suite #101San Antonio, TX 78250(210) 409-9101WebsiteDirectionsMore Info12. The Rising Phoenix VaperyDirectionsPipes & Smokers Articles3730 NW Loop 410San Antonio, TX 78229(210) 236-6537WebsiteDirectionsMore Info13. Get Your Vape OnDirectionsCigar, Cigarette & Tobacco Dealers13212 Huebner RdSan Antonio, TX 78230(210) 492-2819WebsiteDirectionsMore Info14. The Vapor RoomDirections(2)Pipes & Smokers Articles5886 De Zavala Rd Ste 108San Antonio, TX 78249(210) 254-9348WebsiteDirectionsMore Info15. Gabriel's Liquor & Wine SuperstoreDirections(2)Liquor StoresBeer & Ale1309 N Loop 1604 WSan Antonio, TX 78258(210) 492-8585WebsiteDirectionsMore Info16. VaporescenceDirections(3)Cigar, Cigarette & Tobacco-Wholesale...8377 Perrin Beitel RdSan Antonio, TX 78218(210) 654-8273DirectionsMore Info17. Hi TimesDirections(2)Cigar, Cigarette & Tobacco Dealers425 SW Military DrSan Antonio, TX 78221(210) 921-1118DirectionsMore Info18. Thanks For Vaping E-CigsDirections(1)Consumer Electronics16350 Blanco Rd Suite 117San Antonio, TX 78232(210) 479-2770WebsiteDirectionsMore Info19. Thanks For Vaping E-Cigs 2(1)Consumer Electronics7303 San Pedro Ave #101San Antonio, TX 78216(210) 979-8273WebsiteMore Info20. Finck Cigar CoDirections(1)Cigar, Cigarette & Tobacco-Wholesale...414 Vera CruzSan Antonio, TX 78207(210) 226-4191WebsiteDirectionsMore Info21. Monster VapeDirections(1)Tobacco502 Embassy OaksSan Antonio, TX 78216(210) 265-3045WebsiteDirectionsMore Info22. Spirit Of San AntonioDirections(1)Cigar, Cigarette & Tobacco Dealers801 E River Walk StSan Antonio, TX 78205(210) 224-8700WebsiteDirectionsMore Info23. Fincks Cigar Factory OutletDirections(1)Cigar, Cigarette & Tobacco-Wholesale...6100 West AveSan Antonio, TX 78213(210) 341-8888WebsiteDirectionsMore Info24. Planet K EastDirections(1)Cigar, Cigarette & Tobacco Dealers2138 Austin HwySan Antonio, TX 78218(210) 654-8536WebsiteDirectionsMore Info25. Smoker'z ParadizeDirections(1)Cigar, Cigarette & Tobacco Dealers2808 Thousand Oaks DrSan Antonio, TX 78232(888) 403-7638WebsiteDirectionsMore Info26. Spec's LiquorDirections(1)Beer & AleLiquor Stores5219 De Zavala RdSan Antonio, TX 78249(210) 561-0900WebsiteDirectionsMore Info27. Spec's Wines Spirits & FinerDirections(1)Liquor StoresBeer & AleWine5219 De Zavala RdSan Antonio, TX 78249(210) 561-0900WebsiteDirectionsMore Info28. Smokerz ParadizeDirections(2)Cigar, Cigarette & Tobacco Dealers6620 Fm 78San Antonio, TX 78244(210) 662-4183WebsiteDirectionsMore Info29. EZ Discount TobaccoDirections(2)Pipes & Smokers Articles3334 E Southcross BlvdSan Antonio, TX 78223(210) 531-9005DirectionsMore Info30. Lazy Daze Counter CultureDirections(2)Clothing Stores502 Embassy OaksSan Antonio, TX 78216(210) 494-4420WebsiteDirectionsMore InfoGet Your Vape OnDirectionsCigar, Cigarette & Tobacco Dealers13212 Huebner RdSan Antonio, TX 78230(210) 492-2819WebsiteDirectionsMore InfoAdYour Vaping StoreDirectionsGift Shops8015 Bandera Rd Suite #101San Antonio, TX 78250(210) 409-9101WebsiteDirectionsMore InfoAdPuff E-cig(1)Cigar & Cigarette Accessories-Wholesale...Serving the San Antonio Area.(888) 287-8192WebsiteMore InfoAdStella Blues Best Priced Vapor Cigarettes & E-Cigarettes(27)Smokers Information & Treatment CentersServing the San Antonio Area.(888) 280-8174WebsiteCouponsShop OnlineMore InfoAdVape DojoDirections(1)Smokers Information & Treatment CentersServing the San Antonio Area.(888) 279-5831WebsiteFind a LocationMore InfoAdLive Well E-CigCigar & Cigarette Accessories-Wholesale...Serving the San Antonio Area.(888) 309-2305WebsiteCouponsMore InfoAdLizard Juice E-Cigarette(8)Cigar, Cigarette & Tobacco DealersServing the San Antonio Area.(888) 280-5116WebsiteCouponsShop OnlineVirtual TourMore InfoAdBIG DADDYS VAPOR TRAIL & MercantileDirections(1)Cigar, Cigarette & Tobacco DealersServing the San Antonio Area.(888) 538-8466WebsiteShop OnlineMore InfoAdSponsored LinksImprove Our ResultsYou can help improve our service by giving us some details.
First, tell us about yourself:AdvertiserBusiness Owner (non-advertiser)ConsumerEmployeeWhy wasn't this listing relevant to your search?Too far awayExpected more popular resultDuplicate listingNot what I meant by my searchOther/CommentsSubmitCancelor Update This ListingThis particular listing needs to be updated, for example:This business has an incorrect address or phone numberThis business is closedThis business is in the wrong categoryThis business does not existPlease use the Improve Business Info Form »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?1.Club Humidor(2)7074 San Pedro AveSan Antonio, TX 78216(210) 824-1209Cigar, Cigarette & Tobacco DealersWebsiteMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?2.Club Humidor(1)11745 W Interstate 10San Antonio, TX 78230(210) 558-7700Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?3.Club Humidor(2)2235 Thousand Oaks DrSan Antonio, TX 78232(210) 828-1261Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?4.Club Humidor(1)12003 Huebner RdSan Antonio, TX 78230(210) 558-3022Pipes & Smokers ArticlesWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?5.Club Humidor(1)204 Alamo Plz Ste JSan Antonio, TX 78205(210) 472-2875Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?6.The Humidor22806 US Highway 281 NSan Antonio, TX 78258(210) 404-0707Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?7.The Republic Cigar Humidor & Lounge2336 Gruene Lake DrNew Braunfels, TX 78130(830) 626-2525Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?8.San Antonio Vape Club7870 Culebra Rd. Ste. 24San Antonio, TX 78251(210) 689-0787Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?9.Callaghan Smoke Shop Inc(3)4310 Callaghan RdSan Antonio, TX 78228(210) 434-2038Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?10.SA VAPORS(3)14910 Nacogdoches Rd, #101San Antonio, TX 78247(210) 646-8273Pipes & Smokers ArticlesWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAd11.Your Vaping Store8015 Bandera Rd Suite #101San Antonio, TX 78250(210) 409-9101Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?12.The Rising Phoenix Vapery3730 NW Loop 410San Antonio, TX 78229(210) 236-6537Pipes & Smokers ArticlesWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAd13.Get Your Vape On13212 Huebner RdSan Antonio, TX 78230(210) 492-2819Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAd14.The Vapor Room(2)5886 De Zavala Rd Ste 108San Antonio, TX 78249(210) 254-9348Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?15.Gabriel's Liquor & Wine Superstore(2)1309 N Loop 1604 WSan Antonio, TX 78258(210) 492-8585Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?16.Vaporescence(3)8377 Perrin Beitel RdSan Antonio, TX 78218(210) 654-8273Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?17.Hi Times(2)425 SW Military DrSan Antonio, TX 78221(210) 921-1118Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?18.Thanks For Vaping E-Cigs(1)16350 Blanco Rd Suite 117San Antonio, TX 78232(210) 479-2770Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?19.Thanks For Vaping E-Cigs 2(1)7303 San Pedro Ave #101San Antonio, TX 78216(210) 979-8273Cigar, Cigarette & Tobacco DealersWebsiteMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?20.Finck Cigar Co(1)414 Vera CruzSan Antonio, TX 78207(210) 226-4191Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?21.Monster Vape(1)502 Embassy OaksSan Antonio, TX 78216(210) 265-3045Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?22.Spirit Of San Antonio(1)801 E River Walk StSan Antonio, TX 78205(210) 224-8700Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?23.Fincks Cigar Factory Outlet(1)6100 West AveSan Antonio, TX 78213(210) 341-8888Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?24.Planet K East(1)2138 Austin HwySan Antonio, TX 78218(210) 654-8536Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?25.Smoker'z Paradize(1)2808 Thousand Oaks DrSan Antonio, TX 78232(888) 403-7638Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?26.Spec's Liquor(1)5219 De Zavala RdSan Antonio, TX 78249(210) 561-0900Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?27.Spec's Wines Spirits & Finer(1)5219 De Zavala RdSan Antonio, TX 78249(210) 561-0900Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?28.Smokerz Paradize(2)6620 Fm 78San Antonio, TX 78244(210) 662-4183Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?29.EZ Discount Tobacco(2)3334 E Southcross BlvdSan Antonio, TX 78223(210) 531-9005Cigar, Cigarette & Tobacco DealersDirectionsMore InfoRooms & Rates »Add to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?30.Lazy Daze Counter Culture(2)502 Embassy OaksSan Antonio, TX 78216(210) 494-4420Cigar, Cigarette & Tobacco DealersWebsiteDirectionsMore InfoRooms & Rates »Sponsored LinksShowing1-30
of 118results1234NextIf we're missing a business and you'd like to make a suggestion, please do! Add a business.Add a New CollectionOops! There was a problem saving the new custom collection.Please try again.Collection Name cannot be emptyUpload a Photo(optional)Remaining Characters: 100Remaining Characters: 500CancelSave234567891011121314151617182021222324252627282930Map ViewFeatured Cigar, Cigarette & Tobacco Dealersin San Antonio, TexasAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Get Your Vape On13212 Huebner RdSan Antonio, TX 78230(210) 492-2819WebsiteDirectionsMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Your Vaping Store8015 Bandera Rd Suite #101San Antonio, TX 78250(210) 409-9101Contact Us For InformationWebsiteDirectionsMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Puff E-cig(1)Serving the San Antonio Area.(888) 287-8192WebsiteMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Stella Blues Best Priced Vapor Cigarettes & E-Cigarettes(27)Serving the San Antonio Area.(888) 280-8174Made in USA*FDA Approved Ingredients*1800+FB LikesWebsiteCouponsShop OnlineMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Vape Dojo(1)Serving the San Antonio Area.(888) 279-5831WebsiteFind a LocationMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Live Well E-CigServing the San Antonio Area.(888) 309-2305Order Online Now, See Current Specials!WebsiteCouponsMore InfoAdd to mybookRemove from mybookAdded to your other collection!Error when adding to other collectionThis business was removed from the other collectionAdInaccurate Result?Lizard Juice E-Cigarette(8)Serving the San Antonio Area.(888) 280-5116Order OnlineWebsiteCouponsShop OnlineVirtual TourMore InfoAdd to mybookRemove from mybookAdded to your shopping collection!Error when adding to shopping collectionThis business was removed from the shopping collectionAdInaccurate Result?BIG DADDYS VAPOR TRAIL & Mercantile(1)Serving the San Antonio Area.(888) 538-8466Contact Us For More Info!WebsiteShop OnlineMore InfoSponsored San Antonio Club humidorAdPhoneWebsiteMoreAboutAbout YPSite FeedbackContact usAdvertise with UsCareers - We're HiringEngineering BlogLegal | Terms of Service and UsePrivacy PolicySmall Business AdvertisingAdvertising ChoicesAbout Call RecordingSite DirectoryNews & ArticlesFind a BusinessMaps & DirectionsWhite PagesMobile AppsReverse Phone LookupSite MapBrowse RestaurantsMobile YPCity GuidesMore Cities »AtlantaDetroitMiamiAustinHoustonMilwaukeeBaltimoreIndianapolisNew YorkBostonKansas CityOklahoma CityCharlotteLas VegasOrlandoChicagoLos AngelesPhiladelphiaDallasLouisvillePhoenixDenverMemphisSaint LouisYP FamilyCorporate SiteAdvertising SolutionAnyWhoAT&T FamilyAT&TAT&T WirelessLike us on FacebookFollow us on Twitter+1 us on Google+© 2014 YP Intellectual Property LLC. All rights reserved.YP, the YP logo and all other YP marks contained herein are trademarks of YP Intellectual Property LLC and/or YP affiliated companies.AT&T, the AT&T Logo and all AT&T related marks are trademarks of AT&T Inc. or AT&T affiliated companies. All other marks contained herein are the property of their respective owners.We just redesigned yp.com! Do you like it?× LikeNot a Fan× Thank You!Tell us more... »Collections(7)Following(0)Businesses(0)Notes(0)Coupons(0)CollectionsFollowingBusinessesNotesCouponsFeedback

value: This website is a United States-based web site operated by YP Holdings that provides listings for local businesses. In 2013 it was re-branded as YP.com or simply "YP".


text:
Bob Evans - Kissimmee, FL | Groupon
7411 West Irlo Bronson Memorial Highway, Kissimmee, FL 34747 7411 West Irlo Bronson Memorial Highway, Kissimmee Directions
+14073968599
7411 West Irlo Bronson Memorial Highway,
Fill up on fries and other comfort food at Bob Evans, a savory spot for American cuisine. Both the young and the young-at-heart will dig the family-oriented menu and ambience at this restaurant. Score quick and easy seating for your large group at Bob Evans. Bob Evans offers a free wifi hot spot — perfect for surfing the web or getting a little work done.
The friendly staff at Bob Evans are ready and waiting to cook and serve your favorite American meal. For a classic American dish, head over to the casual establishment of Bob Evans. For highly-rated American cuisine, look no further than Bob Evans.
Walt Disney World Resort (1.1 mi)
Celebration Village (2.7 mi)
Celebration Village (2.8 mi)

value: This website is an American global e-commerce marketplace connecting subscribers with local merchants by offering activities, travel, goods and services in 15 countries. Based in Chicago, Groupon was launched there in November 2008, launching soon after in Boston, New York City and Toronto.


text:
2015-16 SP AUTHENTIC FUTURE WATCH CONNOR HELLEBUYCK RC #225 /999 BGS 9.5 | eBay
2015-16 SP Authentic #225 Connor Hellebuyck RC /999
19-20 SP Authentic CONNOR HELLEBUYCK SIGN OF THE TIMES AUTO
C $271.7234 bids
2015-16 UPPER DECK YOUNG GUNS 15-16 #214 CONNOR HELLEBUYCK JETS BGS 9.5 GEM RC
Details about 2015-16 SP AUTHENTIC FUTURE WATCH CONNOR HELLEBUYCK RC #225 /999 BGS 9.5
2015-16 SP AUTHENTIC FUTURE WATCH CONNOR HELLEBUYCK RC #225 /999 BGS 9.5
373098700400
Hankook 225/60/16 All Season Tires

value: This website is an American multinational e-commerce corporation based in San Jose, California, that facilitates consumer-to-consumer and business-to-consumer sales through its website.


text:
Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display. - PubMed - NCBI
Search: Selection of recombinant anti-SH3 domain antibodies by high-th...
Search: Selection[Title] AND recombinant[Title] AND anti-SH3[Title] AND domain[Title] AND antibodies[Title] AND high-throughput[Title] AND phage[Title] AND display[Title]
Huang H1,2, Economopoulos NO1,2, Liu BA3,4, Uetrecht A1,4, Gu J1,2, Jarvik N1, Nadeem V1,2, Pawson T3,4, Moffat J1,4, Miersch S1,2, Sidhu SS1,2,4.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada, M5S 3E1.
Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada, M5S 3E1.
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada, M5S 3E1.
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada, M5S 3E1.
Antibodies are indispensable tools in biochemical research and play an expanding role as therapeutics. While hybridoma technology is the dominant method for antibody production, phage display is an emerging technology. Here, we developed and employed a high-throughput pipeline that enables selection of antibodies against hundreds of antigens in parallel. Binding selections using a phage-displayed synthetic antigen-binding fragment (Fab) library against 110 human SH3 domains yielded hundreds of Fabs targeting 58 antigens. Affinity assays demonstrated that representative Fabs bind tightly and specifically to their targets. Furthermore, we developed an efficient affinity maturation strategy adaptable to high-throughput, which increased affinity dramatically but did not compromise specificity. Finally, we tested Fabs in common cell biology applications and confirmed recognition of the full-length antigen in immunoprecipitation, immunoblotting and immunofluorescence assays. In summary, we have established a rapid and robust high-throughput methodology that can be applied to generate highly functional and renewable antibodies targeting protein domains on a proteome-wide scale.
SH3 domain; antibodies; high throughput method; phage display
PMC4622221
10.1002/pro.2799
CDR sequences and binding kinetics of Fabs. Positions randomized within each of the CDRS are shown at the top of each column numbered according to IMGT standards. Each Fab is identified with a unique identifier (Fab ID #) used throughout the manuscript. For each SH3 domain antigen, abbreviated protein codes corresponding to the Uniprot recommended gene codes are provided to unambiguously identify the parent protein, and its position relative to the total number of SH3 domains in the parent protein is denoted numerically (e.g., ITSN2-2/5 represents the second of five SH3 domains in the protein ITSN2). Binding parameters (kon, koff, Kd) were determined from kinetic analysis of Fabs binding to immobilized antigens measured by surface plasmon resonance. Sequences are shown for Fabs raised against 12 antigens discussed in detail in the text and other Fab sequences are shown in Supporting Information Figure S2.
Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display
Protein Sci. 2015 Nov;24(11):1890-1900.
Evaluation of Fab specificities. The binding specificities of Fab-phage clones were evaluated by phage ELISAs. Signals were measured for Fab-phage binding to cognate SH3 domains (white bars) or homologous SH3 domains (black bars). Asterisks denote Fab-phage exhibiting high affinities by competitive phage ELISAs (IC50 < 50 nM).
Evaluation of anti-RASA1 fabs in IP and western blot applications. (A) Fabs F003.1 and F003.2 (but not a negative control Fab) immunoprecipitated full-length RASA1 protein from lysates of both untransfected HEK293T cell lysates and from lysates of HEK293T cells transfected with a vector designed to over-express RASA1. Immunoprecipitated RASA1 (MW ∼116 kDa) was visualized using a commercial anti-RASA1 antibody (B4F8). (B) Fabs F003.1 and F003.2, and the commercial anti-RASA1 antibody B4F8, recognize a common 116 kDa species corresponding to the molecular weight of full-length RASA1 in western blot experiments with SDS-PAGE of cell lysates from untransfected HEK293T cells or HEK293T cells transfected with a vector designed to over-express RASA1.
Evaluation of anti-RASA1 Fab F003.2 in immunofluorescence applications. HEK-293T cells transfected with a vector for expression of a GFP-tagged full length RASA1 protein were stained with Fab F003.2 and an anti-FLAG Alexa Fluor 647 secondary (recognizing the FLAG tag on the C-terminus of the Fab light chain). Fluorochrome emission from Fab F003.2 staining of cells transfected with the RASA1 expression construct is shown in “A” (red), emission from the GFP tag shown in “B” (green) and an overlay of the two images shown in “C.” The resultant yellow staining pattern observed upon merger of the two images serves as evidence of co-localization between the GFP fusion tag on RASA1 and Fab F003.2 bound to RASA1, confirming target specificity. HEK293T cells transfected with a vector for expression of a negative control GFP-tagged protein (PTK7) were stained as above. In contrast to the staining observed in cells expressing RASA1, no red fluorochrome emission was observed in cells incubated with Fab F003.2 (panel D), despite that GFP emission confirms robust expression of the control PTK7 fusion protein as observed in “E.” Further, upon merger of the two images, only green pseudo-coloured GFP emission is observed in “F,” further attesting to the specificity of the anti-RASA1 Fab F003.2 for it's target only. For contrast, cells were counter-stained with DAPI and shown in the overlays (C and F).
Cell Surface Display Techniques/methods*
Selection[Title] AND recombinant[Title] AND anti-SH3[Title] AND domain[Title] AND antibodies[Title] AND high-throughput[Title] AND phage[Title] AND display[Title]

value: This website is part of the United States National Library of Medicine , a branch of the National Institutes of Health . It is approved and funded by the government of the United States.


text:
9780060537982: Death in the Cards: A Stain-busting Mystery (The Stain-Busting Mysteries) - AbeBooks - Short, Sharon: 0060537981
Death in the Cards: A Stain-busting Mystery (The Stain-Busting Mysteries)
ISBN 13: 9780060537982
ISBN 10: 0060537981
Sharon Short is also the author of the novel My One Square Inch of Alaska (Penguin Plume) in which a pair of siblings escape the strictures of the 1950s industrial Ohio town on the adventure of a lifetime. Opening chapters of this novel earned Sharon an Ohio Arts Council individual artist's grant and a Montgomery County (Ohio) Arts & Cultural District Literary Artist Fellowship. Sharon is the Literary Life columnist for the Dayton Daily News and is the Executive Director of the renowned Antioch Writers' Workshop in Yellow Springs, Ohio. Additionally, Sharon's book Sanity Check: A Collection of Columns includes 100 reader-favorites of her weekly humor and lifestyle column that ran in the Dayton Daily News for more than a decade. Sharon has also published two mystery series (Josie Toadfern and Patricia Delaney) as well as short stories and essays. Sharon holds a B.A. in English from Wright State University and an M.A. in English from Bowling Green State University. She makes her home in her native state of Ohio.
ISBN 10: 1597220027
ISBN 13: 9781597220026
1. Death In The Cards: A Stain-Busting Mystery
Book Description Book Condition: New. Depending on your location, this item may ship from the US or UK. Bookseller Inventory # 97800605379820000000
2. Death in the Cards: A Stain-Busting Mystery (Paperback)
Book Description HarperCollins Publishers Inc, United States, 2005. Paperback. Book Condition: New. 170 x 104 mm. Language: English . Brand New Book. Laundromat owner and stain removal expert Josie Toadfern knows her tiny twon s dirty clothes and most of its dirty little secrets. But an upcoming Psychic Fair is bringing a brand-new mess to Paradises, Ohio. The arrival of all manner of mystics and soothsayers has raised the dangerous ire of a local evangelist and his minions, as well as the more conservative citizens of Paradise--so it doesn t take a crystal ball to predict that murder will ultimately foul the fair. But Josie couldn t foresee that she d be the one to stubmle upon the body, or that her impeccable stain-sense would embroil her in the nasty homicidal happenings. And suddenly her attempt to psych out a killer has Josie s own future looking very grim indeed. Bookseller Inventory # AAS9780060537982
3. Death in the Cards: A Stain-Busting Mystery (Paperback)
4. Death In The Cards
Book Description Book Condition: New. Bookseller Inventory # 2606296-n
5. Death In The Cards
Book Description Harpercollins, 2005. PAP. Book Condition: New. New Book. Shipped from US within 10 to 14 business days. Established seller since 2000. Bookseller Inventory # VC-9780060537982
6. Death in the Cards Format: Mass Market PB
Book Description Harper Collins Publishers. Book Condition: New. Brand New. Bookseller Inventory # 0060537981
7. Death in the Cards: A Stain-busting Mystery (The Stain-Busting Mysteries)
Book Description Avon. MASS MARKET PAPERBACK. Book Condition: New. 0060537981 Brand New Book. Ships from the United States. 30 Day Satisfaction Guarantee!. Bookseller Inventory # 4238675
8. Death in the Cards: A Stain-busting Mystery (The Stain-Busting Mysteries)
Book Description Avon, 2005. Book Condition: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: "DEATH OF A DOMESTIC DIVA is an immaculate conception, and more importantly, good clean fun!". Bookseller Inventory # ABE_book_new_0060537981
9. Death in the Cards: A Stain-busting Mystery (Toadfern Mysteries)
Book Description Avon, 2005. Mass Market Paperback. Book Condition: New. Bookseller Inventory # DADAX0060537981
10. Death in the Cards: A Stain-Busting Mystery (Stain-Busting Mysteries)
Book Description Avon Books, 2005. Book Condition: New. Bookseller Inventory # TB9780060537982

value: This website is an e-commerce global online marketplace with seven websites that offer books, fine art, and collectables from sellers in over 50 countries. Launched in 1996, it specialises in used, rare and out-of-print books.


text:
Physiological modulation of intestinal motility by enteric dopamine... - PubMed - NCBI
Display Settings:AbstractFormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListApplySend to:Choose DestinationFileClipboardCollectionsE-mailOrderMy BibliographyCitation managerFormatSummary (text)Abstract (text)MEDLINEXMLPMID ListCSVCreate File1 selected item: 16525059FormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListMeSH and Other DataE-mailSubjectAdditional textE-mailDidn't get the message? Find out why...Add to ClipboardAdd to CollectionsOrder articlesAdd to My BibliographyGenerate a file for use with external citation management software.Create File
See comment in PubMed Commons belowJ Neurosci. 2006 Mar 8;26(10):2798-807.Physiological modulation of intestinal motility by enteric dopaminergic neurons and the D2 receptor: analysis of dopamine receptor expression, location, development, and function in wild-type and knock-out mice.Li ZS1, Schmauss C, Cuenca A, Ratcliffe E, Gershon MD.Author information1Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. ZL64@columbia.eduAbstractDopaminergic neurons are present in both plexuses of the murine bowel and are upregulated after extrinsic denervation but play unknown roles in enteric nervous system (ENS) physiology. Transcripts encoding dopamine (DA) receptors D1-D5 were analyzed by reverse transcription-PCR in stomach approximately duodenum approximately ileum approximately proximal > > distal colon. Dissected muscle and myenteric plexus contained transcripts encoding D1-D3 and D5, whereas mucosa contained D1 and D3-D5. D1-D5 expression began in fetal gut [embryonic day 10 (E10)], before the appearance of neurons (E12), and was sustained without developmental regulation through postnatal day 1. In situ hybridization revealed that subsets of submucosal and myenteric neurons contained mRNA encoding D2 or D3. Immunoblots confirmed that D1, D2, and D5 receptor proteins were present from stomach through distal colon. Subsets of submucosal and myenteric neurons were also D1, D2, or D3 immunoreactive. When double labeled by in situ hybridization, these neurons contained mRNA encoding the respective receptors. Total gastrointestinal transit time (TGTT) and colonic transit time (CTT) were measured in mice lacking D2, D3, or D2 plus D3. Both TGTT and CTT were decreased significantly (motility increased) in D2 and D2 plus D3, but not D3, knock-out animals. Mice lacking D2 and D2 plus D3 but not D3 were smaller than wild-type littermates, yet ate significantly more and had greater stool frequency, water content, and mass. Because motility is abnormal when D2 is absent, the net inhibitory DA effect on motility is physiologically significant. The early expression of DA receptors is also consistent with the possibility that DA affects ENS development.PMID: 16525059 [PubMed - indexed for MEDLINE] Free full textSharePublication Types, MeSH Terms, Substances, Grant SupportPublication TypesComparative StudyResearch Support, N.I.H., ExtramuralMeSH TermsAnalysis of VarianceAnimalsAnimals, NewbornBlotting, Western/methodsDopamine/metabolism*Dopamine Plasma Membrane Transport Proteins/metabolismDrinking/geneticsEating/geneticsEmbryo, MammalianGastrointestinal Motility/geneticsGastrointestinal Motility/physiology*Gastrointestinal Tract/cytology*Gastrointestinal Tract/growth & developmentGene Expression/physiology*Gene Expression Regulation, Developmental/geneticsImmunoprecipitation/methodsIn Situ Hybridization/methodsMiceMice, Inbred C57BLMice, KnockoutNeurons/metabolism*RNA, Messenger/biosynthesisReceptors, Dopamine D2/deficiencyReceptors, Dopamine D2/geneticsReceptors, Dopamine D2/metabolism*Receptors, Dopamine D3/deficiencyReceptors, Dopamine D3/geneticsReceptors, Dopamine D3/metabolismReverse Transcriptase Polymerase Chain Reaction/methodsTime FactorsSubstancesDopamine Plasma Membrane Transport ProteinsRNA, MessengerReceptors, Dopamine D2Receptors, Dopamine D3DopamineGrant SupportNS12969/NS/NINDS NIH HHS/United StatesNS15547/NS/NINDS NIH HHS/United StatesLinkOut - more resourcesFull Text SourcesHighWireOther Literature SourcesCOS Scholar UniverseLabome Researcher Resource - ExactAntigen/LabomeAccess more work from the authors - ResearchGateMolecular Biology DatabasesDOPAMINE - HSDBKOMP RepositoryMiscellaneousMouse Genome Informatics (MGI)PubMed Commons home


text: . paul ryan gushed over president obama's skills on the debate stage. >> president obama is a very -- he's a very gifted speaker. the man's been on the national stage for many years. he's an experienced debater. >> shockingly, senior white house adviser david plouffe had the same feelings about governor mitt romney. >> we'd expect it all along that governor romney will have a good night. he's prepared more than any candidate in history and shown himself to be a good debater through the years. >> even the candidates were tamping down expectations yesterday. >> i don't think anyone would suggest the debates aren't important. i can't tell you how important it will be. i don't know what will happen at the debates. >> governor romney is a good debater. i'm just okay. >> the soft performance has gotten so soft it's almost evaporated. but while almost everyone seems to be muting enthusiasm in trying to undersell new jersey governor chris christie is taking a different tack. overselling with gusto. >> come thursday morning, the entire narrative of this race is going to change. we have a candidat Search Results 0 to 49 of about 95 (some duplicates have been removed)First<12 >Last

value: This website is an American digital library with the stated mission of "universal access to all knowledge". It provides free public access to collections of digitized materials, including websites, software applications/games, music, movies/videos, moving images, and millions of books.


text: Ron Kellogg III PicturesLINCOLN, NE - NOVEMBER 2: Quarterback Ron Kellogg III #12 of the Nebraska Cornhuskers leaves the field after defeating Northwestern Wildcats with a hail mary touchdown as time ran out at Memorial Stadium on November 2, 2013 in Lincoln, Nebraska. (Photo by Eric Francis/Getty Images)Ron Kellogg III Photos »9 of 15

value: This website is the official website of ESPN. It is owned by ESPN Internet Ventures, a division of ESPN Inc.


text:
9783893381210 - Alibris
ISBN: 9783893381210
389338121X
9783893381210
1993, Rebel Publishing House GmbH
ISBN-13: 9783893381210
$21.98 $99.05
ISBN: 389338121X
Publisher: Rebel Publishing House GmbH
Alibris ID: 14773819923
by Osho, and Sagar, Ma D. (Editor), and Rimmer, Robert H.
1993, Rebel Pub. House Gmbh
$40.00 $99.05
Publisher: Rebel Pub. House Gmbh
Alibris ID: 13696926452
$44.00 $99.05
Alibris ID: 13693550206
New in new dust jacket. In unopened shrink wrap.
1993, Rebel Publishing House
$48.00 $99.05
Alibris ID: 14815674435
Good. 389338121X.
$87.21 $99.05
Alibris ID: 14676919056
Alibris ID: 13250370448
Very Good. 389338121X Light foxing to leaf ends, otherwise dust jacket and pages are Clean and bright! Overall in Great Condition!
Alibris ID: 14676919839
Alibris ID: 14678914213
1993, Rebel Pub. House
Alibris ID: 14490333457

value: This website is is an online store that sells new books, used books, out-of-print books, rare books, and other media through an online network of independent booksellers.


text: Translations English (3) Chinese (Simplified) (2) License OSI-Approved Open Source (5) Apache License V2.0 (2) Artistic License (1) GNU General Public License version 2.0 (2) GNU General Public License version 3.0 (1) GNU Library or Lesser General Public License version 2.0 (2) Public Domain (1) Programming Language Java (4) C# (2) JSP (1) JavaScript (1) Status Planning (2) Beta (1) Production/Stable (1) OS Windows (5) Grouping and Descriptive Categories (4) 32-bit MS Windows (NT/2000/XP) (1) 64-bit MS Windows (1) All 32-bit MS Windows (1) OS Portable (2) Linux (4) Android (3) Mac (3) Modern (1) Linux (1) Freshness Recently updated (1) Top Apps

value: This website is a web service that offers software consumers a centralized online location to control and manage open-source software projects and research business software. It provides source code repository hosting, bug tracking, mirroring of downloads for load balancing, a wiki for documentation, developer and user mailing lists, user-support forums, user-written reviews and ratings, a news bulletin, micro-blog for publishing project updates, and other features.


text:
Resort Village Domaine Annie Sur Mer, Metis Beach, Canada - Booking.com
Great location - show map Excellent location – rated 9.1/10! (score from 207 reviews)
Rated by guests after their stay at Domaine Annie Sur Mer.
Lock in a great price for Domaine Annie Sur Mer – rated 8.4 by recent guests!
The Reford Gardens are 6.2 miles away. Just 20 minutes’ drive away, guests can shop at Ateliers Plein Soleil handicrafts boutique. Club de Golf Boule Rock is 4 minutes’ drive from this resort.
Domaine Annie Sur Mer has been welcoming Booking.com guests since May 7, 2012
The most recent booking for this property was made on Sept 25 at 2:36 PM from the United States.
Domaine Annie Sur Mer was just booked. Reserve your spot now.
6 Reasons to Choose Domaine Annie Sur Mer
2 properties in Metis Beach
Épicerie André Ratté (4.3 miles)
Gite entre chien et loup has a review score of 9.7
See the 1 best hotels in Metis Beach, based on 207 verified hotel reviews on Booking.com.
Customers who viewed Domaine Annie Sur Mer also viewed:
Located in Sainte-Flavie, Gite entre chien et loup offers a seafront location with a view of the Saint-Lawrence River. It provides an outdoor terrace and barbecue. Free WiFi access available. Score from 144 reviews
Featuring free WiFi, Marée Val (à l'Orée de Mer) offers accommodations in Sainte-Flavie. Free private parking is available on site. Certain accommodations include a sitting area for your convenience. Score from 59 reviews
Featuring antiques from the early 1900s, this former church building in Padoue, Quebec, includes a daily breakfast and is 13 km from Jardins de Métis Gardens. Score from 64 reviews
» View more resort villages in Metis Beach
See all guest reviews for Domaine Annie Sur Mer
Newest Reviews for Domaine Annie Sur Mer
눉A coffe maker would be good.
“Great place to stay on the Gaspésie.”
눉They could have had some sort of sign to show where you check in. That was a little confusing.눇The location was beautiful. Waking up next to the water with the sunrise was perfect. The value for money was off the charts.
눉The bathroom was a bit smelly눇The breakfast was delicious, the view from the balcony is very nice
“Wonderful place, cosy and clean”
눉Waitress to be more polite and understanding.눇it was in the country,
눇beautiful place lots of art
“Really great breakfast! Beautiful motel with cute balcony and & cabins in the woods with a view.”
눉WIFI no Access with strong signal
“Lovely, charming place!”
눇Everything was great. Friendly, comfortable and cozy. The pictures on line do not do it justice, that is why I rated accuracy of information as good, not excellent.
눉Bed was old, small and extremely squeaky.눇Location is great, business is locally owned. Breakfast was good and included in the proce.
“Nice getaway next to the Redford Gardens.”
눇Very nice getaway in the Métis-sur-mer area. Very close to the Redford Gardens (Jardins de Métis). Nice friendly staff and very nice amenities.
“Could be do better”
눉The staff at breakfast were surly and unwelcoming in sharp contrast to reception the previous evening. The showers were uncontrollable and could easily have scalded you.눇The location
눉The mattress was a little too firm IMO.눇Beautiful location, nice vines around the front. The staff were very friendly. We booked a couples massage for the morning of our departure and we were able to keep our things in our room past checkout (it was raining and we were traveling by motorcycle). The inn was very cosy and we enjoyed a couple drinks in the bar. We will be sure to return for a longer stay.
“confortable close to jardins de metis”
눉limited restaurant in the evening눇room and bed were great
The Best of Metis Beach
Click here to see more properties near popular landmarks in Metis Beach
Save up to 50% in Metis Beach!
153921,153392,159002,155448,157289|1,157552,152554,154624,158380,154642,159112,158966,158072,159067,155678,156299,153887|6,151729,157682,157168,157255,153887|8,157029,153097,157100,158924|1,157695,159103,158806,154521,155638,154836,158247,158698,156665|3,157240,158263,156372,156540,156084,157289,153887|2,158904,154030,148662,158353,150716,158898,156018,158770,156665,154679,158571,158626,158185,158904|4,153887,155980,153277,155820,157513,153097|1,154971,157616,156777,152522,158350,157348,155918,158924,158432,153451,153311,153459,153726,156430


text:
Revision as of 19:51, 10 October 2011 (view source) HawkBlade124 (talk | contribs) Newer edit →
−[https://wiki.familysearch.org/en/Massachusetts Massachusetts]+[https://wiki.familysearch.org/en/Massachusetts Massachusetts] −[https://wiki.familysearch.org/en/Massachusetts_Census Massachusetts Census] +[[Massachusetts Census]]
Retrieved from "https://familysearch.org/wiki/en/index.php?title=Massachusetts_State_Census,_1865_(FamilySearch_Historical_Records)&oldid=769249"	Categories: FamilySearch Historical Records Published CollectionsUnited States FamilySearch Historical RecordsFamilySearch Historical RecordsMassachusetts	Navigation menu

value: This website is a nonprofit organization and website offering genealogical records, education, and software. It is operated by The Church of Jesus Christ of Latter-day Saints , and is closely connected with the church's Family History Department.


text:
A residue in MutY important for catalysis ident... [Biochemistry. 2004] - PubMed - NCBI
Display Settings:AbstractFormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListApplySend to:Choose DestinationFileClipboardCollectionsE-mailOrderMy BibliographyCitation managerFormatSummary (text)Abstract (text)MEDLINEXMLPMID ListCSVCreate File1 selected item: 14730969FormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListMeSH and Other DataE-mailSubjectAdditional textE-mail"SPAM" filtering software noticeAdd to ClipboardAdd to CollectionsOrder articlesAdd to My BibliographyGenerate a file for use with external citation management software.Create File
See comment in PubMed Commons belowBiochemistry. 2004 Jan 27;43(3):651-62.A residue in MutY important for catalysis identified by photocross-linking and mass spectrometry.Chepanoske CL1, Lukianova OA, Lombard M, Golinelli-Cohen MP, David SS.Author information 1Department of Chemistry, University of Utah, 315 S 1400 E, Salt Lake City, Utah 84112-0850, USA.AbstractMutY is an adenine glycosylase in the base excision repair (BER) superfamily that is involved in the repair of 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG):A and G:A mispairs in DNA. MutY contains a [4Fe-4S]2+ cluster that is part of a novel DNA binding motif, referred to as the iron-sulfur cluster loop (FCL) motif. This motif is found in a subset of members of the BER glycosylase superfamily, defining the endonuclease III-like subfamily. Site-specific cross-linking was successfully employed to investigate the DNA-protein interface of MutY. The photoreactive nucleotide 4-thiothymidine (4ST) incorporated adjacent to the OG:A mismatch formed a specific cross-link between the substrate DNA and MutY. The amino acid participating in the cross-linking reaction was characterized by positive ion electrospray ionization (ESI) tandem mass spectrometry. This analysis revealed Arg 143 as the site of modification in MutY. Arg 143 and nearby Arg 147 are conserved throughout the endo III-like subfamily. Replacement of Arg 143 and Arg 147 with alanine by site-directed mutagenesis reduces adenine glycosylase activity of MutY toward OG:A and G:A mispairs. In addition, the R143A and R147A enzymes exhibit a reduced affinity for duplexes containing the substrate analogue 2'-deoxy-2'-fluoroadenosine opposite OG and G. Modeling of MutY bound to DNA using an endonuclease III-DNA complex structure shows that these two conserved arginines are located within close proximity to the DNA backbone. The insight from mass spectrometry experiments combined with functional mutagenesis results indicate that these two amino acids in the [4Fe-4S]2+ cluster-containing subfamily play an important role in recognition of the damaged DNA substrate.PMID: 14730969 [PubMed - indexed for MEDLINE] SharePublication Types, MeSH Terms, Substances, Grant SupportPublication TypesResearch Support, U.S. Gov't, P.H.S.MeSH TermsAlanine/geneticsAmino Acid SequenceAmino Acid Substitution/geneticsArginine/geneticsBase Pair MismatchCatalysisChromatography, Liquid/methodsCross-Linking Reagents/chemistry*DNA Glycosylases/chemistry*DNA Glycosylases/geneticsDNA, Bacterial/chemistryEscherichia coli Proteins/chemistry*Escherichia coli Proteins/geneticsMolecular Sequence DataMutagenesis, Site-DirectedNucleic Acid Heteroduplexes/chemistrySpectrometry, Mass, Electrospray Ionization/methodsSubstrate Specificity/geneticsThymidine/analogs & derivatives*Thymidine/chemistryUltraviolet RaysSubstances4-thiothymidineCross-Linking ReagentsDNA, BacterialEscherichia coli ProteinsNucleic Acid HeteroduplexesArginineDNA GlycosylasesmutY adenine glycosylaseAlanineThymidineGrant SupportCA67983/CA/NCI NIH HHS/United StatesP30 CA42014/CA/NCI NIH HHS/United StatesLinkOut - more resourcesFull Text SourcesAmerican Chemical SocietyGale DatabasesOther Literature SourcesCOS Scholar UniverseAccess more work from the authors - ResearchGateMolecular Biology Databases(L)-ARGININE - HSDB(L)-ALANINE - HSDBPubMed Commons home


text:
http://www.booking.com/hotel/at/haus-martina-ehrwald.html
Nice and clean and reasonably close to village centre (8 minutes walk). Hosts were very friendly.
We were welcomed by the owners of the property who were very friendly, helpful and they thanked us for choosing their property for our stay and then gave us a "toast" with schnaps. The rooms were warm and clean and basic - but that was all we wanted. There was a TV and a cooker in the main room, and a double bed and single bed in the other. There was Wi-Fi, the signal was strong, sometimes weakening but it was enough to get us by. The view from our balcony was amazing.
Mousey1960,
Situation idéale au centre du village.
Logement parfait et très bien équipé avec tout le confort nécessaire.
Sehr schöne Wohnung sehr gross sauber es hat alles gepasst Danke
Het appartement (het kelderappartement) was groter dan we dachten. Met een volwaardige keuken i.p.v. de verwachtte kitchenette. Er was ook een houtkachel waar we gebruik van mochten maken, dus dat was heerlijk warm 's avonds. Het is 2 minuten rijden naar de Ehrwalder Almbahn. De eigenaar was alleraardigst. We komen graag nog een keer terug!
Dowine,
Superbe accueil de la part des propriétaires. Equipements et espaces de l'appartement excellents. Situation idéale dans le village.
schöner Blick auf die umliegenden Berge und den Daniel
großes, geräumiges Badezimmer
Gute Küchenausstattung mit Kühlschrank, Backofen und Herd
Der Vermieter ist super nett und gewillt seinen Gästen den bestmöglichen Service zu bieten.
De ruimte van het huis was perfect!
De locatie super, de bus en de bakker om de hoek en schitterend uitzicht.
Ontzettend gastvrije eigenaren, je hoeft maar te bellen en ze staan voor je klaar.
Mvaneekelen,
Lock in a great price for Haus Martina – rated 8.1 by recent guests!
One of our bestsellers in Ehrwald! Haus Martina is a 5-minute walk away from the centre of Ehrwald and has a stop of the free ski bus right in front. All apartments feature a fully equipped kitchen, a TV and mountain views.
Various restaurants and shops can be found in the centre of Ehrwald and a bakery is just 350 feet away from Haus Martina.
The Ehrwalder Alm, Sonnenhang and Wetterstein ski areas are 1,650 feet away. The Grubig Ski Area is 1.9 miles away. The free ski bus leaves every 15 minutes and cross-country skiing trails start right from the house.
Haus Martina has been welcoming Booking.com guests since Jun 19, 2012
Located in the top-rated area in Ehrwald, this property has an excellent location score of 8.4!
The most recent booking for this property was made on Jan 21 at 10:09 AM from Austria.
Family Apartment with Garden (2 Adults + 2 Children) Show prices
6 apartments like Haus Martina were just booked
5 Reasons to Choose Haus Martina
Appartements Sonnblick has a location score of 9.6
See the 20 best vacation rentals in Ehrwald, based on 1,246 verified vacation rental reviews on Booking.com.
“Big appartment. Beautiful view over the mouintans. Close to Zugspitze.Quiet.”
“We were welcomed by the owners of the property who were very friendly, helpful and they thanked us for choosing their property for our stay and then gave us a "toast" with schnaps. The rooms were warm and clean and basic - but that was all we wanted. There was a TV and a cooker in the main room, and a double bed and single bed in the other. There was Wi-Fi, the signal was strong, sometimes weakening but it was enough to get us by. The view from our balcony was amazing.”
Mousey1960United Kingdom
Quietly located at the edge of the forest, Ferienhaus Antonia offers direct access to the Sonnenhang Ski Area. Most recent booking for this hotel was today at 11:07
With views to Zugspitze, Germany's highest mountain, Haus Austria offers apartments with satellite TV, balconies and fully equipped kitchens with coffee machines, toasters and microwaves. Most recent booking for this hotel was yesterday at 04:07
눉The 40 euro cleaning fee for only 2 days stay. I think it should be adjusted according to the number of days spent. No breakfast option.눇Big appartment. Beautiful view over the mouintans. Close to Zugspitze.Quiet.
“Good value place to stay in a nice village.”
눉It could do with some modernising but everything worked well and was clean and warm.눇Nice and clean and reasonably close to village centre (8 minutes walk). Hosts were very friendly.
“Haus Martina, Ehrwald”
눉A microwave in the kitchen area would have been useful.눇We were welcomed by the owners of the property who were very friendly, helpful and they thanked us for choosing their property for our stay and then gave us a "toast" with schnaps. The rooms were warm and clean and basic - but that was all we wanted. There was a TV and a cooker in the main room, and a double bed and single bed in the other. There was Wi-Fi, the signal was strong, sometimes weakening but it was enough to get us by. The view from our balcony was amazing.
193683,175976,191645,193924,194413,195575,184105,192182,195647,189326,196599,190542,195954,190078,195907,185647,195596,195907|6,192528,193868,195662,195564,194824,189695|1,189695|4,193105,191944,195969,194457,188328,191030,191655,194125,194643|2,196355,192119,193126,191773,190781,189326|1,192271,195662|2,189485,195323,196114,194611,195645,193805,174933,184105|2,195548,163545,191443,196289,189311,196058,196699,192743,184430,193675,178210,194394,189316,194746,182830,195864,189695|7,192604,195515,186879,192505,189944,194643,184105|5,194439,192869,195898,190946,196472,189695,189593,185503,195650,194416,191747,196662,195754,196476,196314,191955,194153,194771


text:
C++, how client send list<int> values to server one by one? - Stack Overflow
C++, how client send list<int> values to server one by one? [closed]
Basically, I have a list which contains many different integers, I want to send these data from client to server one value by another?
c++ list tcp int share|improve this question asked Apr 16 '12 at 3:02
closed as not a real question by littleadv, Michael Petrotta, Bill the Lizard♦ Apr 17 '12 at 11:53
You have to be more specific. How do you want to send it? Do you have an established connection with the server? What protocol? Can it be socet or has to webservice.
I want to send the list<int> through TCP, and I've already get the server and client connected. Now, I need send the list<int> data from client to server. I know the method send() is based on char* or char[]. I don't know how to deal with list<int>. Thank you very much
you can turn the list of integers into a single string and parse it out on the other side.
How many integers do you have? It could be good enough to convert int to string (snprintf() f.e) and send each one with send().
I have 25 different values. I have to send the value one by one to the server. Which method should I use to convert it to string?
Apr 16 '12 at 3:36
First of all, unless it's absolutely crucial to send them one by one, you'll nearly always be better off sending an entire list at a time instead.
As to how to send them, you normally want to put the ints together into a buffer, convert each to network order, and then send the entire buffer to the receiver. Though I haven't tested this code, something on this general order should work:
void send_ints(socket dest, std::list<int> const &in) {
std::vector<int> netdata;
std::transform(in.begin(), in.end(), std::back_inserter(netdata), ::ntohl);
send(dest, (char *)&netdata[0], net_data.size() * sizeof(netdata[0]), 0);
Depending one your preferences, you might prefer to use static_cast instead of the C-style case I've used above.
Apr 16 '12 at 3:54
add a comment | Not the answer you're looking for?	Browse other questions tagged c++ list tcp int or ask your own question.	asked
asio :how does server actively send information to client while listening to client at the same time?

value: This website is a question and answer website for professional and enthusiast programmers. It is the flagship site of the Stack Exchange Network, created in 2008 by Jeff Atwood and Joel Spolsky.


text:
9781408128169: Left For Dead: 30 Years On - The Race is Finally Over - AbeBooks - WARD NICK: 1408128160
WARD NICK Left For Dead: 30 Years On - The Race is Finally Over
ISBN 13: 9781408128169
ISBN 10: 1408128160 ISBN 13: 9781408128169
In August of 1979, Nick Ward began the 600-mile course of the UK’s Fastnet Race with perfect weather. Within 48 hours, the deadliest storm in the history of modern sailing had blasted through the Irish Sea. By the time it had passed, it had thrown one of the world’s most prestigious races into bedlam and taken the lives of fifteen sailors. Ward’s boat, Grimalkin, was capsized again and again, and the skipper lost overboard; after hours of struggle, three of the crew fled the boat for the liferaft. Nick and his crewmate Gerry, both injured, unconscious, and presumed dead, were abandoned on the beleaguered yacht. Gerry died a few hours later, and Nick was left alone to face down a storm that has become legendary among sailors and racing fans alike. "Left for Dead" is Nick Ward’s harrowing and inspirational memoir of how he survived that dreadful night. After his dramatic rescue, Ward was overwhelmed by media and decided in 1980 not to speak of the incident again. It wasn’t until this book’s coauthor, Sinead O’Brien, approached him about the story that he began the personal writings that became this book. Here at last is the untold true story of an accident that has intrigued lovers of the sea for almost thirty years.
Nick Ward is the survivor of one of the worst sailing accidents of modern history. He lives in Hamble, England, with his wife. They have one son. Sinead O’Brien is an author and documentary filmmaker living in Dublin.
1. Left For Dead: 30 Years On - The Race is Finally Over (Paperback)
Book Description Bloomsbury Publishing PLC, United Kingdom, 2011. Paperback. Book Condition: New. 2nd Revised edition. Language: English . Brand New Book. The second edition of the Sunday Times Sports Book of the Year is updated with a new chapter describing Nick s eventual completion of the Fastnet Race thirty years after his first, ill-fated attempt. The world-famous Fastnet Race takes yachts from the Isle of Wight to the Fastnet Rock off the southwest coast of Ireland and back. The 1979 race began in perfect conditions, but was soon engulfed by the deadliest storm in the history of modern sailing. By the time it passed, the havoc caused was immeasurable, and fifteen sailors had lost their lives. It had been Nick Ward s childhood ambition to sail in the Fastnet Race, and being asked to join the crew of the 30-foot yacht Grimalkin was a dream come true. But then the storm hit. Grimalkin was capsized again and again. With the skipper lost overboard, after hours of struggle three of the crew decided to abandon the boat for the liferaft. Nick and another crewmember, both unconscious, were left on the beleaguered yacht in the middle of the Irish Sea. Both were presumed dead. Gerry died a few hours later, and Nick was left to face the storm alone. Bookseller Inventory # AA79781408128169
2. Left For Dead: 30 Years On - The Race is Finally Over: The Untold Story of the Tragic 1979 Fastnet Race
Book Description A & C Black Publishers Ltd. Paperback. Book Condition: New. New copy - Usually dispatched within 2 working days. Bookseller Inventory # B9781408128169
3. Left For Dead: 30 Years On - The Race is Finally Over (Paperback)
Book Description Bloomsbury Publishing PLC, United Kingdom, 2011. Paperback. Book Condition: New. 2 Revised edition. Language: English . Brand New Book. The second edition of the Sunday Times Sports Book of the Year is updated with a new chapter describing Nick s eventual completion of the Fastnet Race thirty years after his first, ill-fated attempt. The world-famous Fastnet Race takes yachts from the Isle of Wight to the Fastnet Rock off the southwest coast of Ireland and back. The 1979 race began in perfect conditions, but was soon engulfed by the deadliest storm in the history of modern sailing. By the time it passed, the havoc caused was immeasurable, and fifteen sailors had lost their lives. It had been Nick Ward s childhood ambition to sail in the Fastnet Race, and being asked to join the crew of the 30-foot yacht Grimalkin was a dream come true. But then the storm hit. Grimalkin was capsized again and again. With the skipper lost overboard, after hours of struggle three of the crew decided to abandon the boat for the liferaft. Nick and another crewmember, both unconscious, were left on the beleaguered yacht in the middle of the Irish Sea. Both were presumed dead. Gerry died a few hours later, and Nick was left to face the storm alone. Bookseller Inventory # AA79781408128169
4. Left For Dead: 30 Years On - The Race is Finally Over: The Untold Story of the Tragic 1979 Fastnet Race: The 1979 Fastnet Race - One Man`s Epic Story of Survival
Book Description A & C Black Publishers Ltd. Book Condition: New. 2010. 2nd Revised edition. Paperback. Now available as a paperback, Left for Dead is the tragic and inspirational account of Nick Ward's survival against all the odds. It is the experience of an ordinary man who survived an extraordinary event. It affected him so deeply that for over 25 years his story has remained untold - until now. Num Pages: 320 pages, Colour photo sections. BIC Classification: 3JJPL; BGSA; WSSN3. Category: (G) General (US: Trade). Dimension: 198 x 130 x 23. Weight in Grams: 250. . . . . . Books ship from the US and Ireland. Bookseller Inventory # V9781408128169
5. Left For Dead: 30 Years On - The Race is Finally Over: The Untold Story of the Tragic 1979 Fastnet Race: The 1979 Fastnet Race - One Man`s Epic Story of Survival
Book Description A & C Black Publishers Ltd, 2010. Book Condition: New. 2010. 2nd Revised edition. Paperback. Now available as a paperback, Left for Dead is the tragic and inspirational account of Nick Ward's survival against all the odds. It is the experience of an ordinary man who survived an extraordinary event. It affected him so deeply that for over 25 years his story has remained untold - until now. Num Pages: 320 pages, Colour photo sections. BIC Classification: 3JJPL; BGSA; WSSN3. Category: (G) General (US: Trade). Dimension: 198 x 130 x 23. Weight in Grams: 250. . . . . . . Bookseller Inventory # V9781408128169
Ward Nick O'Brien Sinead
Book Description A & C Black Publishers Ltd. Book Condition: New. pp. 320. Bookseller Inventory # 3310108
7. Left For Dead: 30 Years On - The Race is Finally Over
Book Description A & C Black Publishers Ltd, 2010. Paperback. Book Condition: New. book. Bookseller Inventory # M1408128160
8. Left For Dead: 30 Years On - The Race is Finally Over
Book Description A & C Black, 2010. Paperback. Book Condition: Brand New. In Stock. Bookseller Inventory # __1408128160
Book Description Bloomsbury Publishing PLC, 2010. PAP. Book Condition: New. New Book. Shipped from UK in 4 to 14 days. Established seller since 2000. Bookseller Inventory # FV-9781408128169
Nick Ward, Sinead O&#39;Brien
Book Description A&amp;C Black 2010-09-01, London, 2010. paperback. Book Condition: New. Bookseller Inventory # 9781408128169


value: This website is an American internet retailer selling primarily furniture headquartered in Midvale, Utah, near Salt Lake City.


text:
Magnificat: Cambridge St. John's College Choir: Amazon.co.uk: MP3 Downloads
From the Album Tavener: Song for Athene / Svyati
The Whale + Celtic Requiem › John Tavener
B00461HS8Q,B001TO77NE,B00GLSS8R2,B0028JTUQ6,B001IOUD8Q,B001LZZL3O,B00BKQ029M,B0027SCXEO,B00270C4UK,B001IJW5RS,B004UJ93L4,B004UJJEX6,B0084A5LBG,B004UJGFKG,B004TS74VW,B0084A5JSQ,B0082VCSWW,B004TS9SB6,B0082QC1ZQ
Duration: 7:35 minutesGenres: Classical ASIN: B001LYOGH2
Amazon Bestsellers Rank: 326,719 in MP3 Songs (See Top 100 in MP3 Songs)

value: This website is an American multinational technology company which focuses on e-commerce, cloud computing, digital streaming, and artificial intelligence.


text:
This is a real special place. - Review of Chez Elena, Puerto Vallarta, Mexico - TripAdvisor
This is a real special place. - Chez Elena
“This is a real special place.”
Review of Chez Elena
Chez Elena Matamoros 520, Puerto Vallarta, Mexico +52 322 222 0161
Website Improve this listing Ranked #180 of 999 Restaurants in Puerto Vallarta
Restaurant details Good for: Special occasions, Local cuisine, Scenic view, Romantic Dining options: Dinner, Drinks, Full Bar, Outdoor Seating, Reservations, Seating, Serves Alcohol, Waitstaff There are newer reviews for this restaurant
50 helpful votes “This is a real special place.”
This is a special restaurant. On the top floor-there is a open air place which is great for sunsets. It's called El Nido(the Nest). The view is a bit more cluttered than it was -due to one relatively new condo to the southeast(?). The drinks were very good.As for restaurant-one floor down(from El Nido)-the food is excellent. I would recommend the Chicken Mole.There are lovely stained glass windows by the washrooms.
Helpful? Thank pnicol Report Ask pnicol about Chez Elena
This review is the subjective opinion of a TripAdvisor member and not of TripAdvisor LLC. pnicol has 1 more review of Chez Elena “Superb...”
English first French first Spanish first Any ViajeroPerdido
48 helpful votes “Best margaritas”
After the ordeal that is airport immigration on a Saturday afternoon, I was more than happy to walk up the steps, check into my room, drop my bags, and go back downstairs for my first real Mexican meal in, well, far too long.I had the special, a meat dish of some sort (sorry, the details are fading from my memory, but I do remember it was quite good), along with a margarita to celebrate my arrival. OMG, that margarita... Huge (use two hands to lift), incredibly tasty, and quite strong. I had another margarita for dessert, then (uh-oh) found it surprisingly hard to crawl up to my room and find something to hang onto. This from two margaritas?The following couple of nights I went up to El Nido, the rooftop bar, to watch the sunset with the other guests. Both times I again had a margarita (just one each time; I still needed to function), and both times, they were superb. (BTW, El Nido is open to the public, not just hotel guests.)I've had lots of margaritas in other places around PV, but these may have been the best I've ever had - anywhere.
Helpful? Thank ViajeroPerdido Report Ask ViajeroPerdido about Chez Elena
2 helpful votes “great for the memories”
We took advantage of the wonderful view from the rooftoop bar and Pina Coladas. It's a tough walk up the hill from the Church. Dining was excellent in years past but we decided to pass because the restaurant looked old and tired.
Helpful? Thank Mike S Report Ask Mike S about Chez Elena
This review is the subjective opinion of a TripAdvisor member and not of TripAdvisor LLC. chouia
This spot has much to offer. A beautiful view from the roof top bar "El Nido" which serves the best margaritas in Puerto Vallarta. Perfect way to view the famous PV sunsets. Then down on level to the intimate garden restaurant that offers a varied menu, good service & excellent food. Have never been disappointed in 15 years.Andover, MN
Thank chouia Report Ask chouia about Chez Elena
This review is the subjective opinion of a TripAdvisor member and not of TripAdvisor LLC. Jeanne07
The food is always excellent. Have eaten here many times and never been disappointed. It is quiet and the service is great.
Thank Jeanne07 Report Ask Jeanne07 about Chez Elena

value: This website is an American online travel company that operates a website and mobile app with user-generated content and a comparison shopping website.


text:
Close to wonderful restaurants and the river... - Review of Siena Hotel - TripAdvisor
Close to wonderful restaurants and the river... - Review of Siena Hotel
“The only restaurant we ate at was the River View...”
“Near a table or slot b/c they were so busy due to...”
“The Siena Hotel, Spa Casino offers a very good...”
“River view room was awesome, and possibly the...”
“I ate at the sushi restaurant and was really...”
See the most recent reviews Gdogg2013
39 helpful votes “Local Spot”
Helpful? Thank Gdogg2013 Report Ask Gdogg2013 about Siena Hotel
Dutch first English first French first German first Italian first Swedish first Any PrincessUrain
Level Contributor 7 reviews 12 helpful votes “Treated Like Royalty”
Thank PrincessUrain Report Ask PrincessUrain about Siena Hotel
1 review 1 helpful vote “One stop fun!”
Thank Alan K Report Ask Alan K about Siena Hotel
Helpful? Thank Thomasbighan Report Ask Thomasbighan about Siena Hotel
2 reviews “The best downtown Reno hotel”
Helpful? Thank Jay G Report Ask Jay G about Siena Hotel
#8 of 65 in Reno 9,471 reviews
#2 of 65 in Reno 469 reviews
TripAdvisor is proud to partner with Booking.com, Expedia, Hotwire, Hotels.com, Odigeo, Travelocity, Priceline, Orbitz and Jetsetter so you can book your Siena Hotel reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
Explore similar hotels Circus Circus Hotel and Casino-Reno 4,174 ReviewsShow Prices 0.5 miles Grand Sierra Resort and Casino 9,471 ReviewsShow Prices 1.7 miles Harrah's Reno 2,630 ReviewsShow Prices 0.2 miles See all Reno hotels Browse nearby


text:
Genealogical Record of the Compiler's Branch of the Gerrish Family by John J. Ge | eBay
Details about Genealogical Record of the Compiler's Branch of the Gerrish Family by John J. Ge
Genealogical Record of the Compiler's Branch of the Gerrish Family by John J. Ge
13 Aug, 2017 15:42:16 AEST
182690963912
ISBN: 9781342152459 Language: English
Brand: Palala Press EAN: 9781342152459
MPN: N/A UPC: 9781342152459
Author(s) John J Gerrish
ISBN-10 134215245X
ISBN-13 9781342152459


text:
TransOrangeChainsaw comments on 2014 Star Wars prelim picsjump to contentmy subredditsannouncementsArtAskRedditaskscienceawwblogbookscreepydataisbeautifulDIYDocumentariesEarthPornexplainlikeimfiveFitnessfoodfunnyFuturologygadgetsgamingGetMotivatedgifshistoryIAmAInternetIsBeautifulJokesLifeProTipslistentothismildlyinterestingmoviesMusicnewsnosleepnottheonionOldSchoolCoolpersonalfinancephilosophyphotoshopbattlespicsscienceShowerthoughtsspacesportstelevisiontifutodayilearnedTwoXChromosomesUpliftingNewsvideosworldnewsWritingPromptsedit subscriptionsfront-all-random | Documentaries-IAmA-Jokes-news-todayilearned-worldnews-pics-Futurology-gadgets-explainlikeimfive-Fitness-books-Art-AskReddit-tifu-TwoXChromosomes-Showerthoughts-OldSchoolCool-GetMotivated-food-DIY-sports-mildlyinteresting-nottheonion-askscience-InternetIsBeautiful-LifeProTips-philosophy-UpliftingNews-listentothis-videos-movies-nosleep-science-creepy-dataisbeautiful-WritingPrompts-photoshopbattles-funny-space-television-history-personalfinance-gifs-gaming-EarthPorn-Music-awwmore » legocommentsrelatedwant to join? login or register in seconds|Englishlimit my search to /r/legouse the following search parameters to narrow your results:subreddit:subredditfind submissions in "subreddit"author:usernamefind submissions by "username"site:example.comfind submissions from "example.com"url:textsearch for "text" in urlselftext:textsearch for "text" in self post contentsself:yes (or self:no)include (or exclude) self postsnsfw:yes (or nsfw:no)include (or exclude) results marked as NSFWe.g. subreddit:aww site:imgur.com dogsee the search faq for details.advanced search: by author, subreddit...this post was submitted on 15 Aug 201362 points (90% upvoted)shortlink: remember mereset passwordloginSubmit a new linkSubmit a new text postlegosubscribeunsubscribe83,306 readers168 users here now
a community for 6 yearsmessage the moderatorsMODERATORSkey95Technic FanArsenalOwlOoerAutomateAllTheThingsCode MonkeyDacvak6162632014 Star Wars prelim pics (mintinbox.net)submitted 1 year ago by platy31455 commentssharecancelsorry, this has been archived and can no longer be voted onloading...sorted by: bestbesttopnewhotcontroversialoldrandomyou are viewing a single comment's thread.view the rest of the comments →[–]tdbbode 15 points16 points17 points 1 year ago (3 children)sorry, this has been archived and can no longer be voted onThe small vehicles are really cute.. Like bumpercars.. Gonna get a few of those.. And def the Intercepter.. Love it
[–]TransOrangeChainsaw 1 point2 points3 points 1 year ago (1 child)sorry, this has been archived and can no longer be voted onYeah those missiles are pretty pathetic, but the technic piece on them is one of my favorites. Its a solid detail piece and pretty functional too.
permalinkaboutblogaboutteamsource codeadvertisejobshelpwikiFAQreddiquetterulescontact ustoolsmobilefirefox extensionchrome extensionbuttonswidget<3reddit goldstoreredditgiftsreddit AMA appreddit.tvradio redditUse of this site constitutes acceptance of our User Agreement and Privacy Policy. © 2014 reddit inc. All rights reserved.REDDIT and the ALIEN Logo are registered trademarks of reddit inc.close this windowyou'll need to login or register to do thatcreate a new accountusername:password:verify password:email: (optional)remember mecreate accountsign inusername:password:remember mereset passwordloginwe are super-serious about privacy and never spam. learn more.close this windowinterface languageEnglish [en]العربية [ar]Беларуская мова [be]български език [bg]Bosanski [bs]català [ca]česky [cs]dansk [da]Deutsch [de]Ελληνικά [el]English (Australia) [en-au]English (Canadian) [en-ca]English (Great Britain) [en-gb]English [en-us]Esperanto [eo]español [es]español [es-ar]eesti keel [et]Euskara [eu]فارسی [fa]suomi [fi]français [fr]Gàidhlig [gd]עברית [he]मानक हिन्दी [hi]hrvatski [hr]Magyar [hu]Հայերեն լեզու [hy]Bahasa Indonesia [id]íslenska [is] (*)italiano (Italy) [it]日本語 [ja]ಕನ್ನಡ [kn_IN]한국어 [ko]Latin [la]1337 [leet]LOL [lol]lietuvių kalba [lt]latviešu valoda [lv]Nederlands [nl]Nynorsk [nn]Norsk [no]Arrrrrrrr! [pir]polski [pl]português [pt] (*)português [pt-pt]português brasileiro [pt_BR]română [ro]русский [ru]slovenčina [sk]slovenščina [sl]српски језик [sr]Srpski [sr-la]Svenska [sv]தமிழ் [ta]ภาษาไทย [th]Türkçe [tr]українська мова [uk]Tiếng Việt [vi]中文 [zh] (*) incomplete volunteer to translateπ Rendered by PID 31052 on app-11 at 2014-11-21 16:28:20.042854+00:00 running 33d702b country code: US.

value: This website is an American social news aggregation, web content rating, and discussion website. Registered members submit content to the site such as links, text posts, images, and videos, which are then voted up or down by other members.