tokens: PBMC from normal donors incubated for 48 h in the presence of combination IL-2 + IL-4 had nuclear GR with significantly reduced binding affinity ( GR Kd = 36.1 +/- 1.63 nM , mean +/- SEM ; p = 0.0001 ) as compared with PBMC incubated with medium alone ( GR Kd = 6.74 +/- 0.46 nM ) .