Title: Multimodal Nonlinear Microscope based on a Compact Fiber-format Laser Source,
Abstract: We present a multimodal non-linear optical (NLO) laser-scanning microscope,
based on a compact fiber-format excitation laser and integrating coherent
anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and
two-photon-excitation fluorescence (TPEF) on a single platform. We demonstrate
its capabilities in simultaneously acquiring CARS and SRS images of a blend of
6-{\mu}m poly(methyl methacrylate) beads and 3-{\mu}m polystyrene beads. We
then apply it to visualize cell walls and chloroplast of an unprocessed fresh
leaf of Elodea aquatic plant via SRS and TPEF modalities, respectively. The
presented NLO microscope, developed in house using off-the-shelf components,
offers full accessibility to the optical path and ensures its easy
re-configurability and flexibility.