context: Semiconducting polymers swell in typical organic solvents allowing ion penetration and high transconductance. However, for biosensing applications it is essential to operate in aqueous environments. Due to the non-polar nature of polymers, this limits their ability to swell and thus limits performance in aqueous media. A biphasic electrolyte platform developed by Duong et al. incorporates two immiscible electrolytes, an organic layer in direct contact with the polymer and aqueous layer on top. The former is selected because it allows ion penetration into P3HT so it can operate as an OECT, whilst the less dense aqueous layer interfaces with the biological environment of interest. This development opens the potential to utilise a wide range of organic semiconducting polymers that have been previously developed over the past few decades for transistor and solar cell applications.