Effect of reabsorption of fluorescence on transient absorption measurements.
Elimination of fluorescence reabsorption effects is necessary to obtain reliable kinetic data in fluorescence spectroscopy. This effect must also be considered in transient absorption spectroscopy. We devised two methods to achieve this goal. The first was use of a thin optical cell (<10 μm) rather than a much thicker conventional cuvette in the experimental setup. The second was use of an equation to correct data obtained using a conventional cuvette when there were fluorescence reabsorption effects. These methods were successfully used in sub-nanosecond transient absorption spectroscopy to obtain the kinetics of excimer formation by perylene in toluene.