Assessment included lesion counts in two @-cm regions of interest ( ROIs ) , one on each side of the scalp ( ROI-@ : tUVB weeks @-@ , ROI-@ : tUVB weeks @-@ ) , patient self-assessment and analysis of MMP@ , MMP@ , TGFB@ and COL@A@ mRNA expression by quantitative reverse-transcription polymerase chain reaction .