Monocytes were isolated from randomly selected healthy male volunteers according to each Lp-PLA@ genotype ( wild-type Lp-PLA@ -LSB- Lp-PLA@ ( V/V ) -RSB- , the heterozygous V@F mutation -LSB- LpPLA@ ( V/F ) -RSB- and the homozygous V@F mutation -LSB- Lp-PLA@ ( F/F ) -RSB- ) and differentiated into macrophages .