Serologies for HIV ( using parallel rapid tests ) , herpes simplex virus type @ ( HSV-@ ; using Focus HerpeSelect IgG@ ELISA -LSB- Focus Technologies , Cypress Hill , CA -RSB- ) , and syphilis ( rapid plasma reagin confirmed by Treponema pallidum hemagglutination ) were determined , with plasma HIV-@ RNA and CD@ count in HIV-positive patients .